|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Direct differentiation of atrial and ventricular myocytes from human embryonic stem cells by alternating retinoid signals显示文摘尽管 myocyte 房间移植研究为心肌的梗塞建议了一个有希望的治疗学的潜力,到为心肌的修理的临床的治疗的发展的一个主要障碍是与为移植获得相对同类的室的 myocytes 联系的困难。人的胚胎的干细胞(hESCs ) 是 cardiomyocytes 的有希望的来源。这里,我们报导发信号的 retinoid 在 hESCs 的心脏的区别期间调整 atrial 对室的 myocytes 的命运说明。我们发现少量和 pan-retinoic 酸受体对手 BMS-189453 (RAi ) 显著地增加了 hESCs 的心脏的区别效率。为了调查 retinoid,工作,我们把对待 Noggin+RAi 的文化与对待 Noggin+RA 的文化作比较。我们的结果证明室特定的基因 IRX-4 的表示层次极端地在对待 Noggin+RAi 的文化被提高。MLC-2V,另一个室特定的标记,在对待 Noggin+RAi 的文化,然而并非在对待 Noggin+RA 的文化的 cardiomyocytes 在 cardiomyocytes 的多数被表示。流动 cytometry 分析和 electrophysiological 研究与 64.7 | Qiangzhe Zhang Junjie Jiang Pengcheng Han Qi Yuan Jing Zhang Xiaoqian Zhang Yanyan Xu Henghua Cao Qingzhang Meng Li Chen Tian Tian Xin Wang Pu Li Jurgen Hescheler Guangju Ji Yue Ma | 2011 | Cell Research2011，21，4： | 10 |
| 2 | Morphological responses to water-level fluctuations of two submerged macrophytes, Myriophyllum spicatum and Hydrilla verticillata显示文摘Aims Myriophyllum spicatum and Hydrilla verticillata are common submerged macrophytes in the Yangtze River basin.To investigate their tolerances and adaptations to water-level fluctuations,an experiment was conducted in a pond.Methods We designed five different amplitudes of water-level fluctuations as static,615,630,660 and 690 cm during the 74 days of the experiment.In each amplitude treatment,two cultivation methods were examined as monoculture and mixed culture.Important Findings The results showed that M.spicatum had greater morphological responses to water-level fluctuations than H.verticillata.Fluctuating amplitude had significant effects on branch number,shoot length and root dry weight(DW)of M.spicatum,whereas it only had significant effect on branch number of H.verticillata.Both fluctuating amplitude and cultivation method had significant effects on total DW of M.spicatum,which was higher in monoculture than mixed culture.The total DW of H.verticillata was affected by fluctuating amplitude only,and the largest biomass was in the amplitude of 630 cm.Fruit DW of M.spicatum was largest in the amplitude of 630 cm,high amplitude of water-level fluctuations would inhibit flowering and seed production.Root DW proportion was significantly affected by fluctuating amplitude and cultivation method in both species,and the root DW proportion of M.spicatum was significantly higher in the amplitude-of 690 cm.We conclude that moderate amplitude of water-level fluctuations can promote the distribution and growth of both species,and in order to accelerate the restoration of both species in natural habitats,the optimum amplitude should be keep at 615 to 630 cm. | Xiaoke Zhang Xueqin Liu Qingzhang Ding | 2013 | Journal of Plant Ecology2013，6，1： | 10 |
| 3 | Expression and function of leptin and its receptor in mouse mammary gland显示文摘Leptin is an autocrine and paracrine factor which affects the development of duct, formation of gland alveolus, expression of milk protein gene and onset involution of mammary gland. In order to know the function and mechanism of leptin in mammary gland, the protein expression and localization of leptin and its long form receptor (OB-Rb) were detected by a confocal laser scanning microscope. To study the impacts of leptin on mammary gland and leptin signal transduction pathway in pregnancy-, lacta-tion-and involution-stage mammary gland, explants were cultured and Western blotting was used. The results showed that in the whole development cycle of mammary gland, the expression of leptin and OB-Rb was in positive correlation. In virgin the leptin expression was the highest and then decreased in pregnancy. In lactation the expression of leptin was low and upgraded in involution, and recovered to the original level about virgin on involution 13 d. The localization of leptin and OB-Rb revealed that leptin induced the expression of OB-Rb specifically and controlled the development and physiological function of the mammary gland by binding to OB-Rb. In pregnancy stage, leptin stimulated proliferation and differentiation of ductal epithelial cells by JAK-MAPK signal pathway. In lactation, leptin induced gene expression of β-casein by JAK-STAT5 signal pathway, and in involution leptin induced mammary epithelial cell apoptosis and mammary gland restitution by JAK-STAT3 signal pathway. | LIN Ye LI QingZhang | 2007 | Science China(Life Sciences)2007，50，5： | 7 |
| 4 | A novel xeno-free and feeder-cell-free system for human pluripotent stem cell culture显示文摘While human induced pluripotent stem cells(hiPSCs)have promising applications in regenerative medicine,most of the hiPSC lines available today are not suitable for clinical applications due to contamination with nonhuman materials,such as sialic acid,and potential pathogens from animal-product-containing cell culture systems.Although several xeno-free cell culture systems have been established recently,their use of human fibroblasts as feeders reduces the clinical potential of hiPSCs due to batch-to-batch variation in the feeders and time-consuming preparation processes.In this study,we have developed a xeno-free and feeder-cell-free human embryonic stem cell(hESC)/hiPSC culture system using human plasma and human placenta extracts.The system maintains the self-renewing capacity and pluripotency of hESCs for more than 40 passages.Human iPSCs were also derived from human dermal fibroblasts using this culture system by overexpressing three transcription factors—Oct4,Sox2 and Nanog.The culture system developed here is inexpensive and suitable for large scale production. | Qihui Wang Xiaoning Mou Henghua Cao Qingzhang Meng Yanni Ma Pengcheng Han Junjie Jiang Hao Zhang Yue Ma | 2012 | Protein & Cell2012，3，1： | 6 |
| 5 | Microarray analysis of gene expression profiles in the bovine mammary gland during lactation显示文摘Mammary glands undergo functional and metabolic changes during virgin,lactation and dry periods.A total of 122 genes were identified as differentially expressed,including 79 up-regulated and 43 down-regulated genes during lactation compared with virgin and dry periods.Gene ontology analysis showed the functional classification of the up-regulated genes in lactation,including transport,biosynthetic process,signal transduction,catalytic activity,immune system process,cell death,and positive regulation of the developmental process.Microarray data clarified molecular events in bovine mammary gland lactation. | HOU XiaoMing,LI QingZhang & HUANG TianYing Key Laboratory of Dairy Science of Education Ministry,Northeast Agricultural University,Harbin 150030,China | 2010 | Science China(Life Sciences)2010，53，2： | 6 |
| 6 | Current status and trends in forest genomics显示文摘Forests are not only the most predominant of the Earth's terrestrial ecosystems,but are also the core supply for essential products for human use.However,global climate change and ongoing population explosion severely threatens the health of the forest ecosystem and aggravtes the deforestation and forest degradation.Forest genomics has great potential of increasing forest productivity and adaptation to the changing climate.In the last two decades,the field of forest genomics has advanced quickly owing to the advent of multiple high-throughput sequencing technologies,single cell RNA-seq,clustered regularly interspaced short palindromic repeats(CRISPR)-mediated genome editing,and spatial transcriptomes,as well as bioinformatics analysis technologies,which have led to the generation of multidimensional,multilayered,and spatiotemporal gene expression data.These technologies,together with basic technologies routinely used in plant biotechnology,enable us to tackle many important or unique issues in forest biology,and provide a panoramic view and an integrative elucidation of molecular regulatory mechanisms underlying phenotypic changes and variations.In this review,we recapitulated the advancement and current status of 12 research branches of forest genomics,and then provided future research directions and focuses for each area.Evidently,a shift from simple biotechnology-based research to advanced and integrative genomics research,and a setup for investigation and interpretation of many spatiotemporal development and differentiation issues in forest genomics have just begun to emerge. | Dulal Borthakur Victor Busov Xuan Hieu Cao Qingzhang Du Oliver Gailing Fikret Isik Jae-Heung Ko Chenghao Li Quanzi Li Shihui Niu Guanzheng Qu Thi Ha Giang Vu Xiao-Ru Wang Zhigang Wei Lin Zhang Hairong Wei | 2022 | Forestry Research2022，2，1： | 2 |
| 7 | Research on Model Construction and Technique Support for Integrated Rehabilitation of Rock Desertification in Chaoying Small Watershed of Bijie Test Area of Guizhou Province显示文摘Selecting Chaoying small watershed with representative karst rocky desertification in the Bijie test area of Guizhou Province as an example,according to karst rocky desertification degree and the existing eco-environmental problems in the small watershed,we study developmental model and technique support system for integrated rehabilitation of rocky desertification in the small watershed,and bring forward the development model and technique integration for integrated rehabilitation of rocky desertification,which adapt to the karst areas of Bijie test area,Guizhou Province and even South China. | Wei Qingzhang Gao Shourong Jiao Li Wu Yilan | 2014 | Meteorological and Environmental Research2014，5，8： | 1 |
| 8 | Identification of Differentially Expressed MicroRNAs Duringthe Development of Chinese Murine Mammary Gland显示文摘 | Chunmei W Qingzhang L | 2007 | J Genet Genomics2007，34，11： | 1 |
| 9 | Study on effect of soaking time on the transmission of chloride ion in concrete under drying-wetting cycles显示文摘 | ZHANG Qingzhang GU Xianglin ZHANG Weiping | 2011 | Advanced Ma- terials Research2011，，261263： | 1 |
| 10 | Rational Thoughts on Reconstructing Rural Resident's Living Style显示文摘The construction of rural residents' living style is the fundamental signifi cance of building the new socialist countryside.With the deep development of the urban and rural,rural residents' living style has changed profoundly on the labour,consumption,entertainment,marriage and family,etc.,and the differences of living style between the urban and rural are becoming smaller and smaller.Therefore,this paper suggested to promote the rural residents to build the scientif ic,cultural and healthy modern life style,and put forward the rational development thoughts. | SUN Yujuan XU Jingbo YANG Xue SUN Zhanfeng LI Qingzhang | 2009 | Journal of Northeast Agricultural University(English Edition)2009，16，4： | 1 |
| 11 | The de- sign and implement of intelligent TM card water-meter and its managemenl system 显示文摘 | Chen Qingzhang Wang Peng Chen Rongshu | 2010 | Key Engineering Mate- rials2010，，： | 1 |
| 12 | Numerical modelling for offshore platform cathodic protection-local system显示文摘 | Cao Shengshan Wang Qingzhang Zhang Mangping | 1995 | ChinJ Oceanol Limnol1995，13，1： | 1 |
| 13 | Gene therapy for hemophilia B mice with scAAV8-LPI-hFIX显示文摘 | Wei Lu Qingzhang Zhou Hao Yang Hao Wang Yexing Gu Qi Shen Jinglun Xue Xiaoyan Dong Jinzhong Chen | 2016 | Frontiers of Medicine2016，10，2： | 1 |
| 14 | A multiplex nested PCR assay for the simultaneous detection of genetically modified soybean, maize and rice in highly processed products显示文摘 | Ao Jinxia Li Qingzhang Gao Xuejun Yu Yanbo Li Lu Zhang Minghui | 2011 | Food Control2011，，10： | 1 |
| 15 | Development AMS Device for 14C Measurement and Its Applications显示文摘Carbon is the main component in the aerosol.In order to study the source of carbon,it isnecessary to analyze the 14C in the particles.Accelerator mass spectrometry(AMS)is the mosteffective analytical instrument for measuring 14C.Therefore,the purpose of this work is to develop an AMS device,so as to analyze the source | PANG Yijun ZHAO Qingzhang ZHANG Yuxuan WANG Fangfang MENG Qi YU Bo WU Shaoyong HE Ming | 2017 | Annual Report of China Institute of Atomic Energy2017，，1： | 1 |
| 16 | Detection of Roundup Ready soy in highly processed products by triplex nested PCR显示文摘 | Minghui Zhang Xuejun Gao Yanbo Yu Jinxia Ao Jun Qin Yonghao Yao Qingzhang Li | 2006 | Food Control2006，，10： | 1 |
| 17 | Proprotein convertase subtilisin/kexin type 9 (PCSK9) affects gene expression pathways beyond cholesterol metabolism in liver cells显示文摘 | HongLan LingPang Marsha M.Smith DianeLevitan WeiDing LiLiu LixinShan Vidhi V.Shah MaureenLaverty GladysArreaza QingZhang Nicholas J.Murgolo MarcoHernandez Jonathan R.Greene Eric L.Gustafson Marvin L.Bayne Harry R.Davis Joseph A.Hedrick | 2010 | J. Cell. Physiol2010，，1： | 1 |
| 18 | 显示文摘 | Yang Shuting Zhang Yanfeng Lu Qingzhang | 2000 | Journal of Inorganic Materials2000，15，： | 1 |
| 19 | Soil temperatures controlling creeping bentgrass growth 显示文摘 | Bingru Huang John Pore Qingzhang Xu | 2004 | Turfgrass and Environmental Research Online2004，3，18： | 1 |
| 20 | Expression and localisation of glucose transporter 1 (GLUT1) in Chinese Holstein dairy cow mammary gland at different physiological stages显示文摘 | ZHANG Na LI Qingzhang GAO Xuejun | 2009 | Can J Anim Sci2009，89，4： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员