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| 1 | Erianin,a novel dibenzyl compound in Dendrobium extract,inhibits lung cancer cell growth and migration via calcium/calmodulin-dependent ferroptosis显示文摘Ferroptosis,a novel form of programmed cell death,is characterized by iron-dependent lipid peroxidation and has been shown to be involved in multiple diseases,including cancer.Stimulating ferroptosis in cancer cells may be a potential strategy for cancer therapy.Therefore,ferroptosis-inducing drugs are attracting more attention for cancer treatment.Here,we showed that erianin,a natural product isolated from Dendrobium chrysotoxum Lindl,exerted its anticancer activity by inducing cell death and inhibiting cell migration in lung cancer cells.Subsequently,we demonstrated for the first time that erianin induced ferroptotic cell death in lung cancer cells,which was accompanied by ROS accumulation,lipid peroxidation,and GSH depletion.The ferroptosis inhibitors Fer-1 and Lip-1 but not Z-VAD-FMK,CQ,or necrostatin-1 rescued erianin-induced cell death,indicating that ferroptosis contributed to erianin-induced cell death.Furthermore,we demonstrated that Ca^(2+)/CaM signaling was a critical mediator of erianin-induced ferroptosis and that blockade of this signaling significantly rescued cell death induced by erianin treatment by suppressing ferroptosis.Taken together,our data suggest that the natural product erianin exerts its anticancer effects by inducing Ca^(2+)/CaMdependent ferroptosis and inhibiting cell migration,and erianin will hopefully serve as a prospective compound for lung cancer treatment. | Peng Chen Qibiao Wu Jiao Feng Lili Yan Yitian Sun Shuiping Liu Yu Xiang Mingming Zhang Ting Pan Xiaying Chen Ting Duan Lijuan Zhai Bingtao Zhai Wengang Wang Ruonan Zhang Bi Chen Xuemeng Han Yicong Li Liuxi Chen Ying Liu Xingxing Huang Ting Jin Wenzheng Zhang Hong Luo Xiaohui Chen Yongqiang Li Qiujie Li Guohua Li Qin Zhang Lvjia Zhuo Zuyi Yang Huifen Tang Tian Xie Xiaoping Ouyang Xinbing Sui | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 42 |
| 2 | Prevalence of irritable bowel syndrome in Chinese college and university students assessed using Rome Ⅲ criteria显示文摘AIM:To estimate the prevalence of irritable bowel syndrome(IBS)in college and university students of North China and certain related factors for IBS.METHODS:A total of 2500 students from Shandong University in North China were asked in February-March 2009 to complete questionnaires,including the Rome Ⅲquestionnaire,hospital anxiety and depression scale,and IBS-quality of life questionnaire(IBS-QOL).RESULTS:Among the 2126 students with complete data,the prevalence of IBS was 7.85%according to the RomeⅢcriteria,with a female/male ratio of 1.78:1.Most students had the IBS-constipation subtype (36.5%),followed by IBS-diarrhea subtype(31.1%)and IBS-mixed subtype(23.9%).The students with IBS had a higher anxiety and depression score than those without IBS.Low exercise level and anxiety indicated a high risk for IBS.The mean score of IBS patients was 74.2± 4.242 on the IBS-QOL.CONCLUSION:The prevalence of IBS is 7.85%in Chinese college and university students according to the RomeⅢcriteria.Low exercise level and anxiety may be the risk factors for IBS. | Yan-Yan Dong Xiu-Li Zuo Chang-Qing Li Yan-Bo Yu QiuJie Zhao Yan-Qing Li | 2010 | World Journal of Gastroenterology2010，16，33： | 23 |
| 3 | Preparation, characterization, pharmacokinetics and anticancer effects of PEGylated β-elemene liposomes显示文摘Objective:This study aimed to develop a new polyethylene glycol(PEG)ylatedβ-elemene liposome(PEG-Lipo-β-E)and evaluate its characterization,pharmacokinetics,antitumor effects and safety in vitro and in vivo.Methods:The liposomes were prepared by ethanol injection and high-pressure micro-jet homogenization.Characterization of the liposomes was conducted,and drug content,entrapment efficiency(EE),in vitro release and stability were studied by ultra-fast liquid chromatography(UFLC)and a liquid surface method.Blood was drawn from rats to establish the pharmacokinetic parameters.The anticancer effect was evaluated in a KU-19-19 bladder cancer xenograft model.Histological analyses were performed to evaluate safety.Results:The PEG-Lipo-β-E showed good stability and was characterized as 83.31±0.181 nm in size,0.279±0.004 in polydispersity index(PDI),-21.4±1.06 mV in zeta potential,6.65±0.02 in pH,5.024±0.107 mg/mL inβ-elemene(β-E)content,and 95.53±1.712%in average EE.The Fourier transform infrared spectroscopy(FTIR)and differential scanning calorimetry(DSC)indicated the formation of PEG-Lipo-β-E.Compared to elemene injection,PEG-Lipo-β-E demonstrated a 1.75-fold decrease in clearance,a 1.62-fold increase in half-life,and a 1.76-fold increase in area under the concentration-time curves(AUCs)from 0 hour to 1.5 hours(P<0.05).PEG-Lipo-β-E also showed an enhanced anticancer effect in vivo.Histological analyses showed that there was no evidence of toxicity to the heart,kidney,liver,lung or spleen.Conclusions:The present study demonstrates PEG-Lipo-β-E as a new formulation with ease of preparation,high EE,good stability,improved bioavailability and antitumor effects. | Bingtao Zhai Qibiao Wu Wengang Wang Mingming Zhang Xuemeng Han Qiujie Li Peng Chen Xiaying Chen Xingxing Huang Guohua Li Qin Zhang Ruonan Zhang Yu Xiang Shuiping Liu Ting Duan Jianshu Lou Tian Xie Xinbing Sui | 2020 | Cancer Biology & Medicine2020，17，1： | 6 |
| 4 | An ATF24 peptide-functionalized β-elemene-nanostructured lipid carrier combined with cisplatin for bladder cancer treatment显示文摘Objective:In this study,we aimed to develop an amino-terminal fragment(ATF)peptide-targeted liposome carryingβ-elemene(ATF24-PEG-Lipo-β-E)for targeted delivery into urokinase plasminogen activator receptor-overexpressing bladder cancer cells combined with cisplatin(DDP)for bladder cancer treatment.Methods:The liposomes were prepared by ethanol injection and high-pressure microjet homogenization.The liposomes were characterized,and the drug content,entrapment efficiency,andin vitro release were studied.The targeting efficiency was investigated using confocal microscopy,ultra-fast liquid chromatography,and an orthotopic bladder cancer model.The effects of ATF24-PEG-Lipo-β-E combined with DDP on cell viability and proliferation were evaluated by a Cell Counting Kit-8(CCK-8)assay,a colony formation assay,and cell apoptosis and cell cycle analyses.The anticancer effects were evaluated in a KU-19-19 bladder cancer xenograft model.Results:ATF24-PEG-Lipo-β-E had small and uniform sizes(~79 nm),high drug loading capacity(~5.24 mg/mL),high entrapment efficiency(98.37±0.95%),and exhibited sustained drug release behavior.ATF24-PEG-Lipo-β-E had better targeting efficiency and higher cytotoxicity than polyethylene glycol(PEG)ylatedβ-elemene liposomes(PEG-Lipo-β-E).DDP,combined with ATF24-PEG-Lipo-β-E,exerted a synergistic effect on cellular apoptosis and cell arrest at the G2/M phase,and these effects were dependent on the caspase-dependent pathway and Cdc25C/Cdc2/cyclin B1 pathways.Furthermore,thein vivo antitumor activity showed that the targeted liposomes effectively inhibited the growth of tumors,using the combined strategy.Conclusions:The present study provided an effective strategy for the targeted delivery ofβ-elemene(β-E)to bladder cancer,and a combined strategy for bladder cancer treatment. | Bingtao Zhai Peng Chen Wengang Wang Shuiping Liu Jiao Feng Ting Duan Yu Xiang Ruonan Zhang Mingming Zhang Xuemeng Han Xiaying Chen Qiujie Li Guohua Li Ying Liu Xingxing Huang Wenzheng Zhang Ting Pan Lili Yan Ting Jin Tian Xie Xinbing Sui | 2020 | Cancer Biology & Medicine2020，17，3： | 5 |
| 5 | A Polysaccharide-Degrading Marine Bacterium Flammeovirga sp.MY04 and Its Extracellular Agarase System显示文摘Bacteria of the genus Flammeovirga can digest complex polysaccharides (CPs), but no details have been reported regarding the CP depolymerases of these bacteria. MY04, an agarolytic marine bacterium isolated from coastal sediments, has been identified as a new member of the genus Flammeovirga. The MY04 strain is able to utilize multiple CPs as a sole carbon source and grows well on agarose, mannan, or xylan. This strain produces high concentrations of extracellular proteins (490 mg L-1 ± 18.2 mg L-1 liquid culture) that exhibit efficient and extensive degradation activities on various polysaccharides, especially agarose. These proteins have an activity of 310 U mg-1 ± 9.6 U mg-1 proteins. The extracellular agarase system (EAS) in the crude extracellular enzymes contains at least four agarose depolymerases, which are with molecular masses of approximately 30-70 kDa. The EAS is stable at a wide range of pH values (6.0-11.0), temperatures (0-50℃), and sodium chloride (NaCl) concentrations (0- 0.9 mol L-1). Two major degradation products generated from agarose by the EAS are identified to be neoagarotetraose and neoagarohexaose, suggesting that β-agarases are the major constituents of the MY04 EAS. These results suggest that the Flammeovirga strain MY04 and its polysac-charide-degradation system hold great promise in industrial applications. | HAN Wenjun GU Jingyan YAN Qiujie LI Jungang WU Zhihong GU Qianqun LI Yuezhong | 2012 | Journal of Ocean University of China2012，11，3： | 5 |
| 6 | PCDH17 increases the sensitivity of colorectal cancer to 5-fluorouracil treatment by inducing apoptosis and autophagic cell death显示文摘5-Fluorouracil(5-FU)is known as a first-line chemotherapeutic agent against colorectal cancer(CRC),but drug resistance occurs frequently and significantly limits its clinical success.Our previous study showed that the protocadherin 17(PCDH17)gene was frequently methylated and functioned as a tumor suppressor in CRC.However,the relationship between PCDH17 and 5-FU resistance in CRC remains unclear.Here,we revealed that PCDH17 was more highly expressed in 5-FU-sensitive CRC tissues than in 5-FU-resistant CRC tissues,and high expression of PCDH17 was correlated with high BECN1 expression.Moreover,this expression profile contributed to superior prognosis and increased survival in CRC patients.Restoring PCDH17 expression augmented the 5-FU sensitivity of CRC in vitro and in vivo by promoting apoptosis and autophagic cell death.Furthermore,autophagy played a dominant role in PCDH17-induced cell death,as an autophagy inhibitor blocked cell death to a greater extent than the pancaspase inhibitor Z-VAD-FMK.PCDH17 inhibition by siRNA decreased the autophagy response and 5-FU sensitivity.Mechanistically,we showed that c-Jun NH2-terminal kinase(JNK)activation was a key determinant in PCDH17-induced autophagy.The compound SP600125,an inhibitor of JNK,suppressed autophagy and 5-FU-induced cell death in PCDH17-reexpressing CRC cells.Taken together,our findings suggest for the first time that PCDH17 increases the sensitivity of CRC to 5-FU treatment by inducing apoptosis and JNK-dependent autophagic cell death.PCDH17 may be a potential prognostic marker for predicting 5-FU sensitivity in CRC patients. | Shuiping Liu Haoming Lin Da Wang Qiang Li Hong Luo Guoxiong Li Xiaohui Chen Yongqiang Li Peng Chen Bingtao Zhai Wengang Wang Ruonan Zhang Bi Chen Mingming Zhang Xuemeng Han Qiujie Li Liuxi Chen Ying Liu Xiaying Chen Guohua Li Yu Xiang Ting Duan Jiao Feng Jianshu Lou Xingxing Huang Qin Zhang Ting Pan Lili Yan Ting Jin Wenzheng Zhang Lvjia Zhuo Yitian Sun Tian Xie Xinbing Sui | 2019 | Signal Transduction and Targeted Therapy2019，4，1： | 4 |
| 7 | Hypoxia response element-directed expression of bFGF in dental pulp stem cells improve the hypoxic environment by targeting pericytes in SCI rats显示文摘Cell-based transplantation strategies possess great potential for spinal cord injury(SCI)repair.Basic fibroblast growth factor(bFGF)has been reported to have multiple neuro-promoting effects on developing and adult nervous system of mammals and considered a promising therapy for nerve injury following SCI.Human dental pulp stem cells(DPSCs)are abundant stem cells with low immune rejection,which can be considered for cell replacement therapy.The purpose of this study was to investigate the roles of DPSCs which express bFGF under the regulation of five hypoxia-responsive elements(5HRE)using an adeno-associated virus(AAV-5HRE-bFGF-DPSCs)in SCI repairing model.In this study,DPSCs were revealed to differentiate into CD13^(+)pericytes and up-regulate N-cadherin expression to promote the re-attachment of CD13^(+)pericytes to vascular endothelial cells.The re-attachment of CD13^(+)pericytes to vascular endothelial cells subsequently increased the flow rate of blood in microvessels via the contraction of protuberance.As a result,increased numbers of red blood cells carried more oxygen to the damaged area and the local hypoxia microenvironment in SCI was improved.Thus,this study represents a step forward towards the potential use of AAV-5HRE-bFGF-DPSCs in SCI treatment in clinic. | Sipin Zhu Yibo Ying Yan He Xingxing Zhong Jiahui Ye Zhiyang Huang Min Chen Qiuji Wu Yifan Zhang Ziyue Xiang Yurong Tu Weiyang Ying Jian Xiao Xiaokun Li Qingsong Ye Zhouguang Wang | 2021 | Bioactive Materials2021，6，8： | 2 |
| 8 | Behavioural study of the D-galactose induced aging model in C57BL/6J mice显示文摘 | Haifeng Wei Lin Li Qiujie Song Houxi Ai Jin Chu Wei Li | 2006 | 中国生物学文摘2006，20，3： | 2 |
| 9 | A shear-thinning,ROS-scavenging hydrogel combined with dental pulp stem cells promotes spinal cord repair by inhibiting ferroptosis显示文摘Spinal cord injury(SCI)is a serious clinical disease.Due to the deformability and fragility of the spinal cord,overly rigid hydrogels cannot be used to treat SCI.Hence,we used TPA and Laponite to develop a hydrogel with shear-thinning ability.This hydrogel exhibits good deformation,allowing it to match the physical properties of the spinal cord;additionally,this hydrogel scavenges ROS well,allowing it to inhibit the lipid peroxidation caused by ferroptosis.According to the in vivo studies,the TPA@Laponite hydrogel could synergistically inhibit ferroptosis by improving vascular function and regulating iron metabolism.In addition,dental pulp stem cells(DPSCs)were introduced into the TPA@Laponite hydrogel to regulate the ratios of excitatory and inhibitory synapses.It was shown that this combination biomaterial effectively reduced muscle spasms and promoted recovery from SCI. | Yibo Ying Zhiyang Huang Yurong Tu Qiuji Wu Zhaoyu Li Yifan Zhang Huilei Yu Annian Zeng Hanzhi Huang Jiahui Ye Weiyang Ying Min Chen Zhiyi Feng Ziyue Xiang Qingsong Ye Sipin Zhu Zhouguang Wang | 2023 | Bioactive Materials2023，，4： | 2 |
| 10 | Behavioural study of the d -galactose induced aging model in C57BL/6J mice显示文摘 | Haifeng Wei Lin Li Qiujie Song Houxi Ai Jin Chu Wei Li | 2004 | Behavioural Brain Research2004，，2： | 2 |
| 11 | The clustered regularly interspaced short palindromic repeats/associated proteins system for the induction of gene mutations and phenotypic changes in Bombyx mori显示文摘探查基因变化的一般现象, Bombyx mori,鳞翅类的模型有机体,被选择为试验性的模型。容易检测 phenotypic 变化, piggyBac 系统被利用介绍二标记基因进效率在容易为全部实验引起一个新种类帮助了的蚕,和 23.4% 调换。然后,定期聚类短 palindromic repeats/an 联系了的 interspaced 蛋白质(Cas9 ) 系统被利用。结果证明 Cas9 系统能导致有效基因变化,自从 G 0 个人,基础变化能在 B 被检测。mori;并且变化在不同目标地点上评价,这是多样的。下次,产生了更高的变化率的指向 gRNA2 的地点被选择,并且试验性的结果被枚举。首先,在 G 1 产生的变化比例是 30.1% ,并且一些基因变化没从 G 0 产生被继承;偶尔,第二,基础替换没在氨基酸的顺序导致变化,它减少了 phenotypic 的效率与 genotypic 变化的相比变化。这些结果在蚕基因编辑为 Cas9 系统的更好的使用打了基础。 | Jia Song Jiaqian Che Zhengying You Xiaogang Ye Jisheng Li Lupeng Ye Yuyu Zhang Qiujie Qian Boxiong Zhong | 2016 | Acta Biochimica et Biophysica Sinica2016，48，12： | 2 |
| 12 | Benzene hydrogenation over amorphous NiB/bentonite catalyst and promoting effect of Nd 显示文摘 | ZHANG Rongbin LI Fengyi SHI Qiujie | 2003 | Applied Catalysis A2003，239，： | 1 |
| 13 | The effects of rare earths on supported amorphous Ni-B/Al2O3catalysts显示文摘 | Rongbin Zhang Fengyi Li Qiujie Shi | 2001 | Applied Catalysis A:General2001，205，： | 1 |
| 14 | Behavioural study of the d - galactose induced aging model in C57BL,/6J mice 显示文摘 | Wei Haifeng Li Lin Song Qiujie | 2005 | Behavioural Brain Research2005，157，2： | 1 |
| 15 | al, The effects of rare earths on supported amorphous NiB/Al2O3 catalysts显示文摘 | Li Fengyi Shi Qiujie et | 2001 | Appl Catal A:General2001，205，1： | 1 |
| 16 | Behavioural study of the d -galactose induced aging model in C57BL/6J mice显示文摘 | Haifeng Wei Lin Li Qiujie Song Houxi Ai Jin Chu Wei Li | 2004 | Behavioural Brain Research2004，，2： | 1 |
| 17 | Behavioural study of the D-galactose induced aging model in C57BL/6J mice显示文摘 | Wei Haifeng Li Lin Song Qiujie | | 0，，02： | 1 |
| 18 | Behavioural study of the D-galactose induced aging model in C57BL/6J mice 显示文摘 | Wei Haifeng Li Lin Song Qiujie | 2005 | Behavioural Brain Research2005，157，2： | 1 |
| 19 | Behavioural study of thedgalactose induced aging model in C57BL/6J mice显示文摘 | WEI Haifeng LI Lin SONG Qiujie | 2005 | Behav BrainRes2005，157，2： | 1 |
| 20 | Application of 3D T1-SPACE combined with 3D-TOF sequence for follow-up evaluation of stent-assisted coil embolization for intracranial aneurysm显示文摘Objectives:To assess 3D T1-SPACE combined with 3D-TOF sequence for follow-up evaluation of stent-assisted coil embolization for intracranial aneurysm.Materials and methods:Between Oct 2018 and May 2019,we enrolled 25 patients with intracranial aneurysm who underwent stent-assisted coil embolization.All patients were followed up for 6 to10 months after endovascular treatment(EVT)using 3D-TOF MRA,3D T1-SPACE and DSA to evaluate aneurysm occlusion and parent artery patency.Results:With regards to aneurysm occlusion,the specificity of 3D-TOF MRA was 86.9%(20/23)and the accuracy was 84%(21/25).There was no statistical significance(P=0.409)compared with the DSA.The parent artery by3D-TOF MRA showed that there were 14 patients with grade 3,8 patients with grade 2 and 3 patients with grade1.However,3D T1-SPACE showed that all 25 patients were grade 4,and were clearly displayed without metal artifacts.The comparison of the two MR techniques demonstrated that 3D T1-SPACE was superior to 3D-TOF MRA in the evaluation of parent artery(P<0.001).Conclusions:3D T1-SPACE sequence provides better image quality and higher accuracy for evaluating stented parent arteries compared to TOF-MRA.This study also shows that 3D-TOF MRA has a merit to evaluate aneurysm occlusion.The combination of these two modalities can be used as an optional follow-up evaluation after EVT of intracranial aneurysms. | Qiuji Shao Qiang Li Qiaowei Wu Tianxiao Lin Li Li Kaitao Chang Yingkun He | 2021 | Journal of Interventional Medicine2021，4，2： | 1 |
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