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| 1 | Screening Helicobacter pylori genes induced during infection of mouse stomachs显示文摘AIM:To investigate the effect of in vivo environment on gene expression in Helicobacter pylori(H.pylori) as it relates to its survival in the host.METHODS:In vivo expression technology(IVET) systems are used to identify microbial virulence genes.We modified the IVET-transcriptional fusion vector,pIVET8,which uses antibiotic resistance as the basis for selection of candidate genes in host tissues to develop two unique IVET-promoter-screening vectors,pIVET11 and pIVET12.Our novel IVET systems were developed by the fusion of random Sau3A DNA fragments of H.pylori and a tandem-reporter system of chloramphenicol acetyltransferase and beta-galactosidase.Additionally,each vector contains a kanamycin resistance gene.We used a mouse macrophage cell line,RAW 264.7 and mice,as selective media to identify specific genes that H.pylori expresses in vivo.Gene expression studies were conducted by infecting RAW 264.7 cells with H.pylori.This was followed by real time polymerase chain reaction(PCR) analysis to determine the relative expression levels of in vivo induced genes.RESULTS:In this study,we have identified 31 in vivo induced(ivi) genes in the initial screens.These 31 genes belong to several functional gene families,including several well-known virulence factors that are expressed by the bacterium in infected mouse stomachs.Virulence factors,vacA and cagA,were found in this screen and are known to play important roles in H.pylori infection,colonization and pathogenesis.Their detection validates the efficacy of these screening systems.Some of the identified ivi genes have already been implicated to play an important role in the pathogenesis of H.pylori and other bacterial pathogens such as Escherichia coli and Vibrio cholerae.Transcription profiles of allivi genes were confirmed by real time PCR analysis of H.pylori RNA isolated from H.pylori infected RAW 264.7 macrophages.We compared the expression profile of H.pylori and RAW 264.7 coculture with that of H.pylori only.Some genes such as cag A,vac A,lpx C,mur I,tlp C,trx B,sod B,tnp B,pgi,rbf A and inf B showed a 2-20 fold upregulation.Statistically significant upregulation was obtained for all the above mentioned genes(P < 0.05).tlp C,cag A,vac A,sod B,rbf A,inf B,tnp B,lpx C and mur I were also significantly upregulated(P < 0.01).These data suggest a strong correlation between results obtained in vitro in the macrophage cell line and in the intact animal.CONCLUSION:The positive identification of these genes demonstrates that our IVET systems are powerful tools for studying H.pylori gene expression in the host environment. | Aparna Singh Nathaniel Hodgson Ming Yan Jungsoo Joo Lei Gu Hong Sang Emmalena Gregory-Bryson William G Wood Yisheng Ni Kimberly Smith Sharon H Jackson William G Coleman | 2012 | World Journal of Gastroenterology2012，18，32： | 5 |
| 2 | Intrarectal Amifostine During External Beam Radiation Therapy for Prostate Cancer Produces Significant Improvements in Quality of Life Measured by EPIC Score显示文摘 | Nicole L. Simone Cynthia Ménard Benjamin P. Soule Paul S. Albert Peter Guion Sharon Smith Denise Godette Nancy S. Crouse Linda C. Sciuto Theresa Cooley-Zgela Kevin Camphausen C. Norman Coleman Anurag K. Singh | 2008 | International Journal of Radiation Oncology, Biology, Physics2008，，1： | 2 |
| 3 | The IL-2 receptor β chain(p70):role in mediating signals for LAK,NK,and proliferative activities显示文摘 | Siegel JP Sharon M Smith PL | 1987 | Science1987，238，： | 1 |
| 4 | The ecology of algal biodiesel production显示文摘 | Val H. Smith Belinda S.M. Sturm Frank J. deNoyelles Sharon A. Billings | 2009 | Trends in Ecology & Evolution2009，，5： | 1 |
| 5 | Essential oils with insecticidal activity against larvae of Aedes aegypti (Diptera: Culicidae)显示文摘 | Sharon Smith Vera Diego Fernando Zambrano Stelia Carolina Méndez-Sanchez Fernando Rodríguez-Sanabria Elena E. Stashenko Jonny E. Duque Luna | 2014 | Parasitology Research2014，，7： | 1 |
| 6 | Theory of edges of leaves显示文摘 | Marder M Sharon E Smith S | 2003 | Europhys Lett2003，62，： | 1 |
| 7 | Atypical and Malignant Glomus Tumors: Analysis of 52 Cases, With a Proposal for the Reclassification of Glomus Tumors显示文摘 | Andrew L. Folpe Julie C. Fanburg–Smith Markku Miettinen Sharon W. Weiss | 2001 | The American Journal of Surgical Pathology2001，，1： | 1 |
| 8 | The phylogeny of echinoderm classes based on mitochondrial gene arrangements显示文摘 | Michael J. Smith Allan Arndt Sharon Gorski Elizabeth Fajber | 1993 | Journal of Molecular Evolution1993，，6： | 1 |
| 9 | Intrarectal amifostine suspension may protect against acute proctitis during radiation therapy for prostate cancer: A pilot study显示文摘 | Anurag K. Singh Cynthia Ménard Peter Guion Nicole L. Simone Sharon Smith Nancy Sears Crouse Denise J. Godette Theresa Cooley-Zgela Linda C. Sciuto Jonathan Coleman Peter Pinto Paul S. Albert Kevin Camphausen C. Norman Coleman | 2006 | International Journal of Radiation Oncology Biology Physics2006，，4： | 1 |
| 10 | Inhibition of chlorophyll synthesis inHordeum vulgare by 3-amino 2,3-dihydrobenzoic acid (gabaculin)显示文摘 | Corinne M. Hill Sharon A. Pearson Arnold J. Smith Lyndon J. Rogers | 1985 | Bioscience Reports1985，，9： | 1 |
| 11 | Organizing Web Site Information: Principles and Pratical Experience显示文摘 | Paul McInerney Sharon Smith | 2000 | SIGCHI Bulletin2000，32，： | 1 |
| 12 | Effect of Preoperative Nutritional Deficiency on Mortality After Radical Cystectomy for Bladder Cancer显示文摘 | Justin R. Gregg Michael S. Cookson Sharon Phillips Shady Salem Sam S. Chang Peter E. Clark Rodney Davis C.J. Stimson Monty Aghazadeh Joseph A. Smith Daniel A. Barocas | 2011 | The Journal of Urology2011，，1： | 1 |
| 13 | A Single Institution Review of New Breast Malignancies Identified Solely by Sonography显示文摘 | Tobi Greene Costanza Cocilovo Alison Estabrook Lynn Chinitz Christina Giuliano Sharon Rosenbaum Smith Paul I. Tartter | 2006 | Journal of the American College of Surgeons2006，，6： | 1 |
| 14 | Women and Plant Closings : Unemployment, Re - Employment, and Job Training Enrollment Following Dislocation 显示文摘 | Suzanna D Smith Sharon J Price | 1992 | Journal of Family and Economic Issues1992，，： | 1 |
| 15 | Pediatric Obesity and Gallstone Disease显示文摘 | Corinna Koebnick Ning Smith Mary Helen Black Amy H. Porter Bradley A. Richie Sharon Hudson Deborah Gililland Steven J. Jacobsen George F. Longstreth | 2012 | Journal of Pediatric Gastroenterology and Nutrition2012，，3： | 1 |
| 16 | Pay differentials between federal government and private-sector workers 显示文摘 | Smith Sharon P | 1976 | Industrial and Labor Relations Review1976，，29： | 1 |
| 17 | Intrarectal amifostine suspension may protect against acute proctitis during radiation therapy for prostate cancer: A pilot study显示文摘 | Anurag K. Singh Cynthia Ménard Peter Guion Nicole L. Simone Sharon Smith Nancy Sears Crouse Denise J. Godette Theresa Cooley-Zgela Linda C. Sciuto Jonathan Coleman Peter Pinto Paul S. Albert Kevin Camphausen C. Norman Coleman | 2006 | International Journal of Radiation Oncology Biology Physics2006，，4： | 1 |
| 18 | Intrarectal amifostine suspension may protect against acute proctitis during radiation therapy for prostate cancer: A pilot study显示文摘 | Anurag K. Singh Cynthia Ménard Peter Guion Nicole L. Simone Sharon Smith Nancy Sears Crouse Denise J. Godette Theresa Cooley-Zgela Linda C. Sciuto Jonathan Coleman Peter Pinto Paul S. Albert Kevin Camphausen C. Norman Coleman | 2006 | International Journal of Radiation Oncology, Biology, Physics2006，，4： | 1 |
| 19 | Acute Asthma in the pediatric emergency department显示文摘 | Sharon R Smith Robert C Strank | 1999 | Pediatric Clinics of North America1999，46，： | 1 |
| 20 | Study of Nonoutbreak Giardiasis: Novel Findings and Implications for Research显示文摘 | Paul T. Cantey Sharon Roy Brian Lee Alicia Cronquist Kirk Smith Jennifer Liang Michael J. Beach | 2011 | The American Journal of Medicine2011，，12： | 1 |
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