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| 1 | Cancer metabolism and tumor microenvironment:fostering each other?显示文摘The changes associated with malignancy are not only in cancer cells but also in environment in which cancer cells live.Metabolic reprogramming supports tumor cells’high demand of biogenesis for their rapid proliferation,and helps tumor cells to survive under certain genetic or environmental stresses.Emerging evidence suggests that metabolic alteration is ultimately and tightly associated with genetic changes,in particular the dysregulation of key oncogenic and tumor suppressive signaling pathways.Cancer cells activate HIF signaling even in the presence of oxygen and in the absence of growth factor stimulation.This cancer metabolic phenotype,described firstly by German physiologist Otto Warburg,ensures enhanced glycolytic metabolism for the biosynthesis of macromolecules.The conception of metabolite signaling,i.e.,metabolites are regulators of cell signaling,provides novel insights into how reactive oxygen species(ROS)and other metabolites deregulation may regulate redox homeostasis,epigenetics,and proliferation of cancer cells.Moreover,the unveiling of noncanonical functions of metabolic enzymes,such as the moonlighting functions of phosphoglycerate kinase 1(PGK1),reassures the importance of metabolism in cancer development.The metabolic,microRNAs,and ncRNAs alterations in cancer cells can be sorted and delivered either to intercellular matrix or to cancer adjacent cells to shape cancer microenvironment via media such as exosome.Among them,cancer microenvironmental cells are immune cells which exert profound effects on cancer cells.Understanding of all these processes is a prerequisite for the development of a more effective strategy to contain cancers. | Yiyuan Yuan Huimin Li Wang Pu Leilei Chen Dong Guo Hongfei Jiang Bo He Siyuan Qin Kui Wang Na Li Jingwei Feng Jing Wen Shipeng Cheng Yaguang Zhang Weiwei Yang Dan Ye Zhimin Lu Canhua Huang Jun Mei Hua-Feng Zhang Ping Gao Peng Jiang Shicheng Su Bing Sun Shi-Min Zhao | 2022 | Science China(Life Sciences)2022，65，2： | 5 |
| 2 | Homo-PROTAC mediated suicide of MDM2 to treat non-small cell lung cancer显示文摘The dose-related adverse effects of MDM2-P5 3 inhibitors have caused significant concern in the development of clinical safe anticancer agents.Herein we report an unprecedented homo-PROTAC strategy for more effective disruption of MDM2-P53 interaction.The design concept is inspired by the capacity of sub-stoichiometric catalytic PROTACs enabling to degrade an unwanted protein and the dual functions of MDM2 as an E3 ubiquitin ligase and a binding protein with tumor suppressor P53.The new homo-PROTACs are designed to induce self-degradation of MDM2.The results of the investigation have shown that PROTAC 11 a efficiently dimerizes MDM2 with highly competitive binding activity and induces proteasome-dependent self-degradation of MDM2 in A549 non-small cell lung cancer cells.Furthermore,markedly,enantiomer 11 a-1 exhibits potent in vivo antitumor activity in A549 xenograft nude mouse model,which is the first example of homo-PROTAC with in vivo therapeutic potency.This study demonstrates the potential of the homo-PROTAC as an alternative chemical tool for tumorigenic MDM2 knockdown,which could be developed into a safe therapy for cancer treatment. | Shipeng He Junhui Ma Yuxin Fang Ying Liu Shanchao Wu Guoqiang Dong Wei Wang Chunquan Sheng | 2021 | Acta Pharmaceutica Sinica B2021，11，6： | 4 |
| 3 | Ginsenoside Rh2 suppresses colon cancer growth by targeting the miR-150-3p/SRCIN1/Wnt axis显示文摘Ginsenoside Rh2,which is extracted from ginseng,exerts antitumor activity.Recent studies suggest that Rh2 may suppress the growth of colon cancer(CC)in vitro.However,the underlying mechanism remains unclear.In this study,we identified the relative levels of miR-150-3p in CC tissues and cells by a comprehensive strategy of data mining,computational biology,and real-time reverse transcription PCR(qRT-PCR)experiments.The regulatory effects of miR-150-3p/SRCIN1 on the proliferative and invasive abilities of CC cells are evaluated by CCK-8,EdU,wound healing,and transwell assays.Cell cycle-and apoptosis-related protein levels are assessed by western blot analysis.An in vivo tumor formation assay was conducted to explore the effects of miR-150-3p on tumor growth.Furthermore,bioinformatics and dual luciferase reporter assays are applied to determine the functional binding of miRNA to mRNA of the target gene.Finally,the relationship between Rh2 and miR-150-3p was further verified in SW620 and HCT-116 cells.miR-150-3p is downregulated in CC tissues and cell lines.Functional assays indicate that the upregulation of miR-150-3p inhibits tumor growth both in vivo and in vitro.In addition,SRCIN1 is upregulated in CC and predicts a poor prognosis,and it is the direct target for miR-150-3p.Moreover,the miR-150-3p mimic decreases Topflash/Fopflash-dependent luciferase activity,resulting in the inhibition of Wnt pathway activity.Rh2 can suppress the growth of CC by increasing miR-150-3p expression.Rh2 alleviates the accelerating effect on Wnt pathway activity,cell proliferation/migration,and colony formation caused by miR-150-3p inhibition.Rh2 inhibits the miR-150-3p/SRCIN1/Wnt axis to suppress colon cancer growth. | Shipeng Li Wenfeng Han Qichen He Yang Wang Gang Jin Youcheng Zhang | 2023 | Acta Biochimica et Biophysica Sinica2023，55，4： | 2 |
| 4 | Effect of Gd content on microstructure and mechanical properties of Mg–Gd–Y–Zr alloys under peak-aged condition显示文摘 | H.R. Jafari Nodooshan Wencai Liu Guohua Wu You Rao Chenxu Zhou Shipeng He Wenjiang Ding R. Mahmudi | 2014 | Materials Science & Engineering A2014，，： | 1 |
| 5 | A Mesenchymal-to-Epithelial Transition Initiates and Is Required for the Nuclear Reprogramming of Mouse Fibroblasts显示文摘 | Ronghui Li Jialiang Liang Su Ni Ting Zhou Xiaobing Qing Huapeng Li Wenzhi He Jiekai Chen Feng Li Qiang Zhuang Baoming Qin Jianyong Xu Wen Li Jiayin Yang Yi Gan Dajiang Qin Shipeng Feng Hong Song Dongshan Yang Biliang Zhang Lingwen Zeng Liangxue Lai Miguel | 2010 | Cell Stem Cell2010，，1： | 1 |
| 6 | Autocatalytic reduction-assisted synthesis of segmented porous PtTe nanochains for enhancing methanol oxidation reaction显示文摘Morphology engineering has been developed as one of the most widely used strategies for improving the performance of electrocatalysts.However,the harsh reaction conditions and cumbersome reaction steps during the nanomaterials synthesis still limit their industrial applications.Herein,one-dimensional(1D)novel-segmented PtTe porous nanochains(PNCs)were successfully synthesized by the template methods assisted by Pt autocatalytic reduction.The PtTe PNCs consist of consecutive mesoporous architectures that provide a large electrochemical surface area(ECSA)and abundant active sites to enhance methanol oxidation reaction(MOR).Furthermore,1D nanostructure as a robust sustaining frame can maintain a high mass/charge transfer rate in a long-term durability test.After 2,000 cyclic voltammetry(CV)cycles,the ECSA value of PtTe PNCs remained as high as 44.47 m^(2)·gPt^(-1),which was much larger than that of commercial Pt/C(3.95 m^(2)·gPt^(-1)).The high catalytic activity and durability of PtTe PNCs are also supported by CO stripping test and density functional theory calculation.This autocatalytic reduction-assisted synthesis provides new insights for designing efficient low-dimensional nanocatalysts. | Qiqi Zhang Tianyu Xia He Huang Jialong Liu Mengyuan Zhu Hao Yu Weifeng Xu Yuping Huo Congli He Shipeng Shen Cong Lu Rongming Wang Shouguo Wang | 2023 | Nano Research Energy2023，2，1： | 1 |
| 7 | Inheritance of seeds per silique in Brassica napus L. using joint segregation analysis显示文摘 | Liwu Zhang Pingwu Liu Dengfeng Hong Anqun Huang Shipeng Li Qingbiao He Guangsheng Yang | 2009 | Field Crops Research2009，，1： | 1 |
| 8 | A Mesenchymal-to-Epithelial Transition Initiates and Is Required for the Nuclear Reprogramming of Mouse Fibroblasts显示文摘 | Ronghui Li Jialiang Liang Su Ni Ting Zhou Xiaobing Qing Huapeng Li Wenzhi He Jiekai Chen Feng Li Qiang Zhuang Baoming Qin Jianyong Xu Wen Li Jiayin Yang Yi Gan Dajiang Qin Shipeng Feng Hong Song Dongshan Yang Biliang Zhang Lingwen Zeng Liangxue Lai Miguel | 2010 | Cell Stem Cell2010，，1： | 1 |
| 9 | Ionic Liquid-Stabilized Single-Atom Rh Catalyst Against Leaching显示文摘Single-atom catalysts(SACs)are promising in some reactions typically promoted by homogeneous catalysts.However,the leaching of active species from the support in liquid-phase reactions hinders their potential applications.Herein,taking the hydroformylation reaction as an example,we report ionic liquid(IL)-induced stabilization of single Rh atoms against leaching.Among the ILs examined. | Shipeng Ding Max JHülsey Hua An Qian He Hiroyuki Asakura Min Gao Jun-ya Hasegawa Tsunehiro Tanaka Ning Yan | 2021 | CCS Chemistry2021，3，10： | 1 |
| 10 | High-spin Level Structure of (92)~Nb显示文摘The experiment was performed at the HI-13tandem accelerator in the China Institute of Atomic Energy(CIAE).The high-spin states of(92)^Nb were populated via the heavy-ion fusionevaporation reaction(82)^Se((14)^N,4n)(92)^Nb with beam energy of 54MeV.The target is a 0.99mg/cm2 thick isotopically enriched 82Se metallic foil with 8.27mg/cm2 Yb backing to stop the recoiling nuclei. | ZHENG Yun WU Yiheng WU Xiaoguang LI Congbo ZHU Lihua LI Tianxiao LUO Pengwei LI Guangsheng HE Chuangye LI Hongwei HU Shipeng LIU Jiajian WANG Jinlong YAO Shunhe CHEN Qiming ZHONG Jian | 2018 | 中国原子能科学研究院年报2018，，1： | 0 |
| 11 | Magnetoresistance anomaly in Fe_(5)GeTe_(2)homo-junctions induced by its intrinsic transition显示文摘Two-dimensional van der Waals(2D vdW)magnets have attracted great attention recently and possess the unprecedented advantages of incorporating high-quality vdW heterostructures and homostructures into spintronic devices,and exploring various physical phenomena or technologies.Among them,Fe_(5)GeTe_(2)(F5GT)has ferromagnetic order close to room temperature,however the magnetic properties near its intrinsic transitions and F5GT-based 2D devices remain mostly unexplored.Here,we systematically demonstrate the peculiar magnetic properties of Fe_(5)GeTe_(2)nanoflakes near its intrinsic transition temperature(Tp)which is far lower than its Curie temperature(TC)of~265 K,and firstly discover anomalous magnetoresistance effect in F5GT homo-junctions by magneto-transport measurements.The strongest anomalous Hall effect occurs around Tp which is located in a temperature range from 130 to 160 K for the F5GT nanoflakes with different thicknesses.Furthermore,negative magnetoresistance(N-MR)and butterfly-shaped magnetoresistance(B-MR)are observed in F5GT homo-junction devices,and they appeared only in an intermediate temperature range from 110 to 160 K,noticeably showing the maxima near the T_(p)rather than the lowest temperature.Our experimental results clearly reveal the significant influence of intrinsic transitions on magnetic properties of F5GT and magnetoresistance effect in F5GT homo-junction devices,which imply a new strategy to achieve highperformance 2D spintronic devices by tuning intrinsic magnetic or structural transitions in 2D vdW magnets. | Ruijie Zhao Yanfei Wu Shaohua Yan Xinjie Liu He Huang Yang Gao Mengyuan Zhu Jianxin Shen Shipeng Shen Weifeng Xu Zeyu Zhang Liyuan Zhang Jingyan Zhang Xinqi Zheng Hechang Lei Ying Zhang Shouguo Wang | 2023 | Nano Research2023，16，7： | 0 |
| 12 | Molecular cytogenetic map visualizes the heterozygotic genome and identifies translocation chromosomes in Citrus sinensis显示文摘Citrus sinensis is the most cultivated and economically valuable Citrus species in the world,whose genome has been assembled by three generation sequencings.However,chromosome recognition remains a problem due to the small size of chromosomes,and difficulty in differentiating between pseudo and real chromosomes because of a highly heterozygous genome.Here,we employ fluorescence in situ hybridization(FISH)with 9 chromosome painting probes,30 oligo pools,and 8 repetitive sequences to visualize 18 chromosomes.Then,we develop an approach to identify each chromosome in one cell through single experiment of oligo-FISH and Chromoycin A3(CMA)staining.By this approach,we construct a high-resolution molecular cytogenetic map containing the physical positions of CMA banding and 38 sequences of FISH including centromere regions,which enables us to visualize significant differences between homologous chromosomes.Based on the map,we locate several highly repetitive sequences on chromosomes and estimate sizes and copy numbers of each site.In particular,we discover the translocation regions of chromosomes 4 and 9 in C.sinensis“Valencia.”The high-resolution molecular cytogenetic map will help improve understanding of sweet orange genome assembly and also provide a fundamental reference for investigating chromosome evolution and chromosome engineering for genetic improvement in Citrus. | Shipeng Song Hui Liu Luke Miao Li He Wenzhao Xie Hong Lan Changxiu Yu Wenkai Yan Yufeng Wu Xiao-peng Wen Qiang Xu Xiuxin Deng Chunli Chen | 2023 | Journal of Genetics and Genomics2023，50，6： | 0 |
| 13 | Nuclear peripheral chromatin-lamin B1 interaction is required for global integrity of chromatin architecture and dynamics in human cells显示文摘The eukaryotic genome is folded into higher-order conformation accompanied with constrained dynamics for coordinated genome functions.However,the molecular machinery underlying these hierarchically organized three-dimensional(3D)chromatin architecture and dynamics remains poorly understood.Here by combining imaging and sequencing,we studied the role of lamin B1 in chromatin architecture and dynamics.We found that lamin B1 depletion leads to detachment of lamina-associated domains(LADs)from the nuclear periphery accompanied with global chromatin redistribution and decompaction.Consequently,the interchromosomal as well as inter-compartment interactions are increased,but the structure of topologically associating domains(TADs)is not affected.Using live-cell genomic loci tracking,we further proved that depletion of lamin B1 leads to increased chromatin dynamics,owing to chromatin decompaction and redistribution toward nucleoplasm.Taken together,our data suggest that lamin B1 and chromatin interactions at the nuclear periphery promote LAD maintenance,chromatin compaction,genomic compartmentalization into chromosome territories and A/B compartments and confine chromatin dynamics,supporting their crucial roles in chromatin higher-order structure and chromatin dynamics. | Lei Chang Mengfan Li Shipeng Shao Chen Li Shanshan Ai Boxin Xue Yingping Hou Yiwen Zhang Ruifeng Li Xiaoying Fan Aibin He Cheng Li Yujie Sun | 2022 | Protein & Cell2022，13，4： | 0 |
| 14 | PN-ImTLSM facilitates high-throughput low background single-molecule localization microscopy deep in the cell显示文摘When imaging the nucleus structure of a cell,the out-of-focus fluorescence acts as background and hinders the detection of weak signals.Light-sheet fluorescence microscopy(LSFM)is a wide-field imaging approach which has the best of both background removal and imaging speed.However,the commonly adopted orthogonal excitation/detection scheme is hard to be applied to single-cell imaging due to steric hindrance.For LSFMs capable of high spatiotemporal single-cell imaging,the complex instrument design and operation largely limit their throughput of data collection.Here,we propose an approach for high-throughput background-free fluorescence imaging of single cells facilitated by the Im-mersion Tilted Light Sheet Microscopy(ImTLSM).ImTLSM is based on a light-sheet projected off the optical axis of a water immersion objective.With the illumination objective and the detection objective placed opposingly,ImTLSM can rapidly patrol and optically section multiple individual cells while maintaining single-molecule detection sensitivity and resolution.Further,the simplicity and robustness of ImTLSM in operation and maintenance enables high-throughput image collection to establish background removal datasets for deep learning.Using a deep learning model to train the mapping from epi-illumination images to ImTLSM illumination images,namely PN-ImTLSM,we demonstrated crossmodality fluorescence imaging,transforming the epi-illumination image to approach the background removal performance obtained with ImTLSM.We demonstrated that PN-ImTLSM can be generalized to large-field homogeneous illumination imaging,thereby further improving the imaging throughput.In addition,compared to commonly used background removal methods,PN-ImTLSM showed much better performance for areas where the background intensity changes sharply in space,facilitating high-density single-molecule localization microscopy.In summary,PN-ImTLSM paves the way for background-free fluorescence imaging on ordinary inverted microscopes. | Boxin Xue Caiwei Zhou YizhiQin Yongzheng Li Yuao Sun Lei Chang Shipeng Shao Yongliang Li Mengling Zhang Chaoying Sun Renxi He Qian Peter Su Yujie Sun | 2021 | Biophysics Reports2021，7，4： | 0 |
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