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| 1 | The HuangZaoSi Maize Genome Provides Insights into Genomic Variation and Improvement History of Maize显示文摘Maize is a globally important crop that was a classic model plant for genetic studies. Here, we report a 2.2 Gb draft genome sequence of an elite maize line, HuangZaoSi (HZS). Hybrids bred from HZS-improved lines (HILs) are planted in more than 60% of maize fields in China. Proteome clustering of six completed sequeneed maize genomes show that 638 proteins fall into 264 HZS-specific gene families with the majority of contributions from tandem duplication events. Resequencing and comparative analysis of 40 HZSrelated lines reveals the breeding history of HILs. More than 60% of identified selective sweeps were clustered in identity.by.descent conserved regions, and yield-related genes/QTLs were enriched in HZS characteristic selected regions. Furthermore, we dem on strated that HZS-specific family genes were not uniformly distributed in the genome but enriched in improvement/function.related genomic regions. This study provides an important and novel resource for maize genome research and expands our knowledge on the breadth of genomic variation and improvement history of maize. | Chunhui Li Wei Song Yingfeng Luo Shenghan Gao Ruyang Zhang Zi Shi Xiaqing Wang Ronghuan Wang Fengge Wang Jidong Wang Yanxin Zhao Aiguo Su Shuai Wang Xin Li Meijie Luo Shuaishuai Wang Yunxia Zhang Jianrong Ge Xinyu Tan Ye Yuan Xiaochun Bi Hang He Jianbing Yan Yuandong Wang Songnian Hu Jiuran Zhao | 2019 | Molecular Plant2019，12，3： | 15 |
| 2 | Effect of molecular weight of dissolved organic matter on toxicity and bioavailability of copper to lettuce显示文摘To clarify the effects of molecular weight of dissolved organic matter (DOM) on the toxicity and bioavailability of copper (Cu) to plants,DOM extracted from chicken manure was ultra-filtered into four fractions according to their molecular weights by means of sequential-stage ultrafiltration technique.Lettuce seeds were germinated by being exposed to the solutions containing Cu 2+ with or without different fractions of DOM.The concentration of copper in roots,leaves,sprouts and the length of roots were investigated.The results showed that not all fractions of DOM could improve copper availability or toxicity.The fraction of DOM with larger molecular weight more than 1 kDa had higher complexation stability with Cu 2+ and caused lower concentration of free Cu 2+ ion in the solution of copper plus the fraction,resulting in lower availability and toxicity of copper to lettuce,but the fraction with molecular weight less than 1 kDa had the opposite function.Therefore,the molecular weight of 1 kDa may be the division point to determine DOM to increase or decrease copper availability and toxicity. | Xudong Wang,Xianni Chen,Shuai Liu,Xizu Ge College of Resource and Environment,Northwest Agriculture and Forestry University,Shaanxi 712100,China | 2010 | Journal of Environmental Sciences2010，22，12： | 8 |
| 3 | Endocrine Disruption Activity of 30-day Dietary Exposure to Decabromodiphenyl Ethane in Balb/C Mouse显示文摘Objective This study aimed to evaluate the hepatotoxicity,metabolic disturbance activity and endocrine disrupting activity of mice treated by Decabromodiphenyl ethane(DBDPE).Methods In this study,Balb/C mice were treated orally by gavage with various doses of DBDPE.After 30 days of treatment,mice were sacrificed; blood,livers and thyroid glands were obtained,and hepatic microsomes were isolated.Biochemical parameters including 8 clinical chemistry parameters,blood glucose and hormone levels including insulin and thyroid hormone were assayed.The effects of DBDPE on hepatic cytochrome P450(CYP) levels and activities and uridinediphosphate-glucuronosyltransferase(UDPGT) activities were investigated.Liver and thyroid glands were observed.Results There were no obvious signs of toxicity and no significant treatment effect on body weight,or liver-to-body weight ratios between treatment groups.The levels of ALT and AST of higher dose treatment groups were markedly increased.Blood glucose levels of treatment groups were higher than those of control group.There was also an induction in TSH,T3,and f T3.UDPGT,PROD,and EROD activities were found to have been increased significantly in the high dose group.Histopathologic liver changes were characterized by hepatocyte hypertrophy and cytoplasmic vacuolization.Our findings suggest that DBDPE can cause a certain degree of mouse liver damage and insufficiency.Conclusion DBDPE has the activity of endocrine disruptors in Bal/C mice,which may induce drug-metabolizing enzymes including CYPs and UDPGT,and interfere with thyroid hormone levels mediated by Ah R and CAR signaling pathways.Endocrine disrupting activity of DBDPE could also affect the glucose metabolism homeostasis. | SUN Ru Bao SHANG Shuai ZHANG Wei LIN Ben Cheng WANG Qiang SHI Yun XI Zhu Ge | 2018 | Biomedical and Environmental Sciences2018，31，1： | 7 |
| 4 | Experiment and simulation on the pyrite removal from the recirculating load of pulverizer with a dilute phase gas-solid fluidized bed显示文摘In order to reduce the energy consumption and subsequent air pollution of coal-fired power station, based on the analysis to size and density distribution of particles from the recirculating load of the classifier of pulverizer, the separation experiment on sampling material from power plant with a dilute phase fluidized bed to remove pyrite and other minerals and numerical simulation on the separation process were done. The results show that the minimum fluidization velocity is 1.62 cm/s. Pyrite and other minerals in the material are separated. Ash of the upper and bottom layer material account for 33.34% and 73.42% respectively and sulfur content occupy 1.12% and 8.96% respectively. Scanning electron microscopy and spectroscopy tests show that sulfur in the bottom material exist in the form of pyrite. Numerical simulation on the flow field form of the dilute phase separation bed with gas-solid two phase and particle motion verifies the experimental results. | Wang Shuai He Yaqun He Jingfeng Ge Linhan Liu Qing | 2013 | International Journal of Mining Science and Technology2013，23，2： | 6 |
| 5 | Breakage and separation mechanism of ZGM coal mill based on parameters optimization model显示文摘Sampling ports were firstly drilled on a ZGM95 coal mill in the power plant in China, and the coal samples from various points in the pulverizer were collected under the different operation conditions. The property of the sampling material from the mill was analyzed, applying the float–sink test, size distribution analysis, proximate analysis and so on. It was indicated that the +250 lm fraction in the pulverized fuel accounted for only 0.02%, while it was 83.2% in the new feed. The circulating ratio and coal flow in the separator and the cone zone were calculated using the mass balance of the circulating load. So, the circulating ratio in the separator of the pulverizer was between 8 and 13, and the circulating ratio, the feed flow of separator and cone zone all raised with the increase of the air volume. Furthermore, the parameters of the separation functions were obtained based on the fitting method. It was shown that the mean value of the shape factor B was 0.7617, and the parameter D which is the particle size at 50% cumulative yield in the separator almost kept unchanged. | Wei Hua He Yaqun Shi Fengnian Zhou Nianxing Wang Shuai Ge Linhan | 2014 | International Journal of Mining Science and Technology2014，24，2： | 5 |
| 6 | Nanocluster PtNiP supported on graphene as an efficient electrocatalyst for methanol oxidation reaction显示文摘In this study,phosphorus doped graphene supported PtNiP nanocluster electrocatalyst(PtNiP/P-graphene)was successfully prepared via a simple hypophosphite-assisted co-reduction method.The improved anchoring force and increased anchoring sites of graphene support result from phosphorus doping as well as size-confined growth effect of NaH_(2)PO_(2) leads to uniform dispersion of ultrafine PtNiP nanoclusters.Doped P also promotes the removal of CO-like intermediate by adjusting Pt electronic structure combining with alloyed Ni via electronic effects.As a result,the as-prepared PtNiP/P-graphene catalyst with more exposed active sites and optimized electronic structure of Pt alloy shows excellent electrocatalytic performances for methanol oxidation reaction(MOR)both in activity and durability in an acidic medium. | Long Yang Guoqiang Li Rongpeng Ma Shuai Hou Jinfa Chang Mingbo Ruan Wenbin Cai Zhao Jin Weilin Xu Guiling Wang Junjie Ge Changpeng Liu Wei Xing | 2021 | Nano Research2021，14，8： | 4 |
| 7 | Influences of agricultural phenology dynamic on land surface biophysical process and climate feedback显示文摘到气候变化的土地表面过程的反应和反馈是地球科学的领域里的研究优先级之一。学习在陆地表面过程上对全球变化的影响给予了更多的注意的水流,而是到气候变化的陆地表面过程的反馈是糟糕理解。系统地理解在农业物候学之间的关系在地球系统科学的框架下面正在变得越来越有意义气候上的动态、生物物理的过程,以及反馈。在这篇论文,我们在这个领域里总结了研究进步,包括农业物候学变化,在土地表面进步模型动态的物候学的 parameterization,对气候的在生物物理的过程上动态的农业物候学的影响,以及它的反馈的事实。结果证明农业 phenophase,由象播种, flowering 和成熟那样的关键 phenological 阶段代表了,由于气候变化和农学的管理的影响显著地变了。土地的数字表情出现动态过程和大气的过程,被联合在土地动态的物候学改进表面模型。农业物候学动态影响了网放射,潜伏的热,理智的热,反照率,温度,降水,循环,在划分的表面精力和气候反馈起一个重要作用。认为在陆地表面动态的农业物候学的重要性是生物物理的过程和气候反馈,下列研究优先级应该被强调:(1 ) 在气候之间的相互作用变化并且登陆表面物候学动态;(2 ) 在动态的农业物候学和土地之间的关系出现在不同系列的反射率;(3 ) 土地的庄稼生理学特征变化的贡献出现生物物理的过程;(4 ) 从在不同气候地区动态的物候学的气候反馈的地区性的差别。这评论是有用的加速在陆地表面过程和气候反馈动态的农业物候学的角色的理解。 | LIU Fengshan CHEN Ying SHI Wenjiao ZHANG Shuai TAO Fulu GE Quansheng | 2017 | Journal of Geographical Sciences2017，27，9： | 3 |
| 8 | Electrostatic-lenses position-sensitive TOF MCP detector for beam diagnostics and new scheme for mass measurements at HIAF显示文摘A foil–microchannel plate(MCP)detector,which uses electrostatic lenses and possesses both good position and timing resolutions,has been designed and simulated for beam diagnostics and mass measurements at the next-generation heavy-ion-beam facility HIAF in China.Characterized by low energy loss and good performances of timing and position measurements,it would be located at focal planes in fragment separator HFRS for position monitoring,beam turning,Bq measurement,and trajectory reconstruction.Moreover,it will benefit the building-up of a magnetic-rigidity–energy-loss–time-offlight(BqDETOF)method at HFRS for high-precision in-flight particle identification of radioactive isotope beams on an event-by-event basis.Most importantly,the detector can be utilized for in-ring TOF and position measurements,beam-line TOF measurements at two achromatic foci,and position measurements at a dispersive focus of HFRS,thus making it possible to use two complementary mass measurement methods[isochronous mass spectrometry at the storage ring SRing and magnetic-rigidity–time-of-flight(BqTOF)at the beam-line HFRS]in one single experimental run. | Jun-Hao Liu Zhuang Ge Qian Wang Geng Wang Li-Na Sheng Wen-Wen Ge Xing Xu Peng Shuai Qi Zeng Bo Wu | 2019 | Nuclear Science and Techniques2019，30，10： | 3 |
| 9 | Investigation of soil and groundwater environment in urban area during post-industrial era: A case study of brownfield in Zhenjiang, Jiangsu Province, China显示文摘With the adjustment of industrial structure,many high pollution enterprises will gradually shut down.This process will produce a large number of high-risk pollution plots and cause a series of environmental problems.In this study,geophysical detection and direct push technology were applied to investigate soil and groundwater pollution in a typical brownfield in Zhenjiang,Jiangsu province,China.The results showed that Chlorotoluene and its ramification were the main pollutants for the brownfield.Pollution decreased with depth and was quite uneven in the brownfield.Production and wastewater treatment areas were heavily polluted,where volatile organic compounds contaminated and semi-volatile organic compounds were found 12 m and 15 m below the land surface,respectively.About 18880 m3 groundwater was contaminated.This study is significant to develop a geological survey work mode for the postindustrial era. | Shuai Yang Wei-ya Ge Hong-han Chen Wen-li Xu | 2019 | China Geology2019，2，4： | 3 |
| 10 | Direct observation of chaotic resonances in optical microcavities显示文摘Optical microcavities play a significant role in the study of classical and quantum chaos.To date,most experimental explorations of their internal wave dynamics have focused on the properties of their inputs and outputs,without directly interrogating the dynamics and the associated mode patterns inside.As a result,this key information is rarely retrieved with certainty,which significantly restricts the verification and understanding of the actual chaotic motion.Here we demonstrate a simple and robust approach to directly and rapidly map the internal mode patterns in chaotic microcavities.By introducing a local index perturbation through a pump laser,we report a spectral response of optical microcavities that is proportional to the internal field distribution.With this technique,chaotic modes with staggered mode spacings can be distinguished.Consequently,a complete chaos assisted tunneling(CAT)and its time-reversed process are experimentally verified in the optical domain with unprecedented certainty. | Shuai Wang Shuai Liu Yilin Liu Shumin Xiao Zi Wang Yubin Fan Jiecai Han Li Ge Qinghai Song | 2021 | Light(Science & Applications)2021，10，8： | 3 |
| 11 | Molecular detection of SARS-CoV-2 being challenged by virus variation and asymptomatic infection显示文摘Coronavirus disease 2019(COVID-19)has been a pandemic for more than a year.With the expanding second wave of the pandemic in winter,the continuous evolution of SARS-CoV-2 has brought new issues,including the significance of virus mutations in infection and the detection of asymptomatic infection.In this review,we first introduced several major SARS-CoV-2 mutations since the COVID-19 outbreak and then mentioned the widely used molecular detection techniques to diagnose COVID-19,primarily focusing on their strengths and limitations.We further discussed the effects of viral genetic variation and asymptomatic infection on the molecular detection of SARS-CoV-2 infection.The review finally summarized useful insights into the molecular diagnosis of COVID-19 under the special situation being challenged by virus mutation and asymptomatic infection. | Congshan Jiang Xiaowei Li Changrong Ge Yuanyuan Ding Tao Zhang Shuai Cao Liesu Meng Shemin Lu | 2021 | Journal of Pharmaceutical Analysis2021，11，3： | 2 |
| 12 | Inversion of the three-dimensional temperature structure of mesoscale eddies in the Northwest Pacific based on deep learning显示文摘Mesoscale eddies,which are mainly caused by baroclinic effects in the ocean,are common oceanic phenomena in the Northwest Pacific Ocean and play very important roles in ocean circulation,ocean dynamics and material energy transport.The temperature structure of mesoscale eddies will lead to variations in oceanic baroclinity,which can be reflected in the sea level anomaly(SLA).Deep learning can automatically extract different features of data at multiple levels without human intervention,and find the hidden relations of data.Therefore,combining satellite SLA data with deep learning is a good way to invert the temperature structure inside eddies.This paper proposes a deep learning algorithm,eddy convolution neural network(ECN),which can train the relationship between mesoscale eddy temperature anomalies and sea level anomalies(SLAs),relying on the powerful feature extraction and learning abilities of convolutional neural networks.After obtaining the temperature structure model through ECN,according to climatic temperature data,the temperature structure of mesoscale eddies in the Northwest Pacific is retrieved with a spatial resolution of 0.25°at depths of 0–1000 m.The overall accuracy of the ECN temperature structure is verified using Argo profiles at the locations of cyclonic and anticyclonic eddies during 2015–2016.Taking 10%error as the acceptable threshold of accuracy,89.64%and 87.25%of the cyclonic and anticyclonic eddy temperature structures obtained by ECN met the threshold,respectively. | Fangjie Yu Zeyuan Wang Shuai Liu Ge Chen | 2021 | Acta Oceanologica Sinica2021，40，10： | 2 |
| 13 | Divergence in flowering time is a major component contributing to reproductive isolation between two wild rice species(Oryza rufipogon and O. nivara)显示文摘It is of critical importance for our understanding of speciation process to determine the forms of reproductive isolation and their relative importance in species divergence. Oryza nivara and O. rufipogon are direct ancestors of Asian cultivated rice and a progenitor-daughter species pair. Investigating the reproductive isolation between them provides insights into plant speciation and helps understanding of the rice domestication. Here, we quantitatively measured the major components of reproductive isolation between the two species based on common garden and crossing experiments for three pairs of sympatric populations in Nepal, Cambodia and Laos.We revealed significant differences in the flowering times between species pairs, with O. nivara flowering much earlier than O. rufipogon. A very weak reduction in seed set but no reduction in F1 viability and fertility were detected for the crosses between species relative to those within species. Moreover, we detected asymmetrical compatibility between species and found that emasculation significantly decreased pollination success in O. nivara but not in O. rufipogon.Our study demonstrates that the divergence between O. nivara and O. rufipogon is maintained almost entirely by the difference in flowering times and suggests that differential flowering times contribute to both habitat preferences and reproductive isolation between species. | Xun Xu Qing-Lin Meng Mu-Fan Geng Ning-Ning Ren Lian Zhou Yu-Su Du Zhe Cai Mei-Xia Wang Xin Wang Xiu-Hua Wang Jing-Dan Han Shuai Jiang Chun-Yan Jing Rong Liu Xiao-Ming Zheng Qing-Wen Yang Fu-Min Zhang Song Ge | 2020 | Science China(Life Sciences)2020，63，11： | 2 |
| 14 | Structural and functional basis for pan-CoV fusion inhibitors against SARS-CoV-2 and its variants with preclinical evaluation显示文摘The COVID-19 pandemic poses a global threat to public health and economy.The continuously emerging SARS-CoV-2 variants present a major challenge to the development of antiviral agents and vaccines.In this study,we identified that EK1 and cholesterol-coupled derivative of EK1,EK1C4,as pan-CoV fusion inhibitors,exhibit potent antiviral activity against SARS-CoV-2 infection in both lung-and intestine-derived cell lines(Calu-3 and Caco2,respectively).They are also effective against infection of pseudotyped SARS-CoV-2 variants B.1.1.7(Alpha)and B.l.1.248(Gamma)as well as those with mutations in S protein,including N417T,E484K,N501Y,and D614G,which are common in South African and Brazilian variants.Crystal structure revealed that EK1 targets the HR1 domain in the SARS-CoV-2 S protein to block virus-cell fusion and provide mechanistic insights into its broad and effective antiviral activity.Nasal administration of EK1 peptides to hACE2 transgenic mice significantly reduced viral titers in lung and intestinal tissues.EK1 showed good safety profiles in various animal models,supporting further clinical development of EK1-based pan-CoV fusion inhibitors against SARS-CoV-2 and its variants. | Shuai Xia Qiaoshuai Lan Yun Zhu Chao Wang Wei Xu Yutang Li Lijue Wang Fanke Jiao Jie Zhou Chen Hua Qian Wang Xia Cai Yang Wu Jie Gao Huan Liu Ge Sun Jan Munch Frank Kirchhoff Zhenghong Yuan Youhua Xie Fei Sun Shibo Jiang Lu Lu | 2021 | Signal Transduction and Targeted Therapy2021，6，8： | 2 |
| 15 | Development of fluorine-substituted NH_(2)-biphenyl-diarylpyrimidines as highly potent non-nucleoside reverse transcriptase inhibitors:Boosting the safety and metabolic stability显示文摘Our recent studies for nonnucleoside reverse transcriptase inhibitors identified a highly potent compound JK-4b against WT HIV-1(EC_(50)=1.0 nmol/L),but the poor metabolic stability in human liver microsomes(t_(1/2)=14.6 min)and insufficient selectivity(SI=2059)with high cytotoxicity(CC_(50)=2.08μmol/L)remained major issues associated with JK-4b.The present efforts were devoted to the introduction of fluorine into the biphenyl ring of JK-4b,leading to the discovery of a novel series of fluorine-substituted NH_(2)-biphenyl-diarylpyrimidines with noticeable inhibitory activity toward WT HIV-1 strain(EC_(50)=1.8–349 nmol/L).The best compound 5t in this collection(EC_(50)=1.8 nmol/L,CC_(50)=117μmol/L)was 32-fold in selectivity(SI=66,443)compared to JK-4b and showed remarkable potency toward clinically multiple mutant strains,such as L100I,K103N,E138K,and Y181C.The metabolic stability of 5t was also significantly improved(t_(1/2)=74.52 min),approximately 5-fold higher than JK-4b in human liver microsomes(t_(1/2)=14.6 min).Also,5t possessed good stability in both human and monkey plasma.No significant in vitro inhibition effect toward CYP enzyme and hERG was observed.The single-dose acute toxicity test did not induce mice death or obvious pathological damage.These findings pave the way for further development of 5t as a drug candidate. | Xin Jin Shuai Wang Limin Zhao Wenjuan Huang Yinxiang Zhang Christophe Pannecouque Erik De Clercq Ge Meng Huri Piao Fener Chen | 2023 | Acta Pharmaceutica Sinica B2023，13，3： | 1 |
| 16 | A biologically inspired solution to simultaneous localization and consistent mapping in dynamic environments显示文摘 | Yangming Li Shuai Li Yunjian Ge | 2012 | Neurocomputing2012，，： | 1 |
| 17 | Influence of the low-frequency source parameters on the plasma characteristics in a dual frequency capacitively coupled plasma reactor:Two dimensional simulations显示文摘A two-dimensional (2D) fluid model is presented to study the discharge of argon in a dual frequency capacitively coupled plasma (CCP) reactor. We are interested in the influence of low-frequency (LF) source parameters such as applied voltage amplitudes and low frequencies on the plasma characteristics. In this paper, the high frequency is set to 60 MHz with voltage 50 V. The simulations were carried out for low frequencies of 1, 2 and 6 MHz with LF voltage 100 V, and for LF voltages of 60, 90 and 120 V with low frequency 2 MHz. The results of 2D distributions of electric field and ion density, the ion flux impinging on the substrate and the ion energy on the powered electrode are shown. As the low frequency increases, two sources become from uncoupling to coupling. When two sources are uncoupling, the increase in LF has little impact on the plasma characteristics, but when two sources are coupling, the increase in LF decreases the uniformities of ion density and ion flux noticeably. It is also found that with the increase in LF voltage, the uniformities in the radial direction of ion density distribution and ion flux at the powered electrode decreases significantly, and the energy of ions bombarding on the powered electrode increases significantly. | Xiang Xu, Hao Ge, Shuai Wang, Zhongling Dai, Younian Wang , Aimin Zhu State Key Lab of Materials Modification by Electron, Ion and Laser Beams, School of Physics and Optoelectronic Technology, Dalian University of Technology, Dalian 116023, China | 2009 | Progress in Natural Science:Materials International2009，19，6： | 1 |
| 18 | Production of 5-Hydroxymethylfurfural from Fructose in Ionic Liquid Efficiently Catalyzed by Cr(III)-Al_(2)O_(3)Catalyst显示文摘A series of metal-Al_(2)O_(3)catalysts were prepared simply by the conventional impregnation with Al_(2)O_(3)and metal chlorides,which were applied to the dehydration of fructose to 5-hydroxymethylfurfural(HMF).An agreeable HMF yield of 93.1%was achieved from fructose at mild conditions(100℃and 40 min)when employing Cr(III)-Al_(2)O_(3)as catalyst in 1-butyl-3-methylimidazolium chloride([Bmim]Cl).The Cr(III)-Al_(2)O_(3)catalyst was characterized via XRD,DRS and Raman spectra and the results clarified the interaction between the Cr(III)and the alumina support.Meanwhile,the reaction solvents([Bmim]Cl)collected after 1st reaction run and 5th reaction run were analyzed by ICP-OES and LC-ITMS and the results confirmed that no Cr(III)ion was dropped off from the alumina support dur-ing the fructose dehydration.Notably,Cr(III)-Al_(2)O_(3)catalyst had an excellent catalytic performance for glucose and sucrose and the HMF yields were reached to 73.7%and 84.1%at 120℃for 60 min,respectively.Furthermore,the system of Cr(III)-Al_(2)O_(3)and[Bmim]Cl exhibited a constant stability and activity at 100℃for 40 min and a fa-vorable HMF yield was maintained after ten recycles. | Changhua Song Hui Liu Yuan Li Shuai Ge Hongwei Wang Wenshuai Zhu Yonghui Chang Changri Han Huaming Li | 2014 | Chinese Journal of Chemistry2014，32，5： | 1 |
| 19 | Determination of Residual Amount of 15 Plant Growth Regulators in Bean Sprouts by HPLC-MS/MS显示文摘[Objectives]This study was conducted to effectively monitor PGR residues in bean sprouts to provide guarantee for the food safety of agricultural products.[Methods]A high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method for the determination of residues of 15 plant growth regulators(PGRs)in bean sprouts was established using bean sprouts as an experimental material.Samples were extracted with a solution containing 5%acetic acid-acetonitrile(1∶99,V/V),purified with anhydrous magnesium sulfate,and diluted with methanol solvent to constant volume.The solutions were filtered through 0.22μm filtering membrane and the target analytes were separated on a Phenomenex H18 column.The identification of each compound was established by retention time matching along with the accurate mass measurement of the precursor ions and their main fragment ions.The quantification was carried out using matrix-matched external standard method.[Results]The retention time of the 15 PGRs were found in the range from 5.8-11.7 min under the optimized conditions.The linear relation was good in the concentration range of 0.005-0.050μg/ml,and the correlation coefficients of the 15 PGRs were≥0.9990.The limits of detection were in the range of 0.03-0.92 g/kg,and the limits of quantification were in the range of 0.50-2.10μg/kg.The average recovery in the recovery test at 3 concentration levels was 80%-110%,and the relative standard deviations were in the range of 2.8%-7.5%.[Conclusions]This method is simple and accurate,and can quickly qualitatively and quantitatively analyze the residues of 15 PGRs in bean sprouts.The proposed procedure was simple,quick and accurate for the simultaneous determination of the 15 PGRs in bean sprout. | Lei WANG Yi ZHANG Baiqin ZHENG Shuai WANG Kai GE Sining TANG Shuhong ZHANG Liwu HAO | 2021 | Agricultural Biotechnology2021，10，6： | 1 |
| 20 | Generation of recombinant rabies virus ERA strain applied to virus tracking in cell infection显示文摘The mechanism of rabies virus (RABV) infection still needs to be further characterized. RABV particle with self-fluorescent is a powerful viral model to visualize the viral infection process in cells. Herein, based on a reverse genetic system of the Evelyn-Rokitnicki-Abelseth (rERA) strain, we generated a recombinant RABV rERA-N/mCherry strain that stably expresses an additional ERA nucleoprotein that fuses with the red fluorescent protein mCherry (N/mCherry). The rERA-N/mCherry strain retained growth property similar to the parent strain rERA in vitro. The N/mCherry expression showed genetic stability during passage into mouse neuroblastoma (NA) cells and did not change the virulence of the vector. The rERA-N/mCherry strain was then utilized as a visual viral model to study the RABV-cell binding and internalization. We directly observed the red self-fluorescence of rERA-N/mCherry particles binding to the cell surface, and further co-localizing with clathrin in the early stage of infection in NA cells by fluorescence microscopy. Our results showed that the rERA-N/mCherry strain uses clathrin-dependent endocytosis to enter cells, which is consistent with the well-known mechanism of RABV invasion. The recombinant RABV rERA-N/mCherry thus appears to have the potential to be an effective viral model to further explore the fundamental molecular mechanism of rabies neuropathogenesis. | ZHAO Dan-dan SHUAI Lei GE Jin-ying WANG Jin-liang WEN Zhi-yuan LIU Ren-qiang WANG Chong WANG Xi-jun BU Zhi-gao | 2019 | Journal of Integrative Agriculture2019，18，10： | 1 |
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