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| 1 | The interaction of CpEBF1 with CpMADSs is involved in cell wall degradation during papaya fruit ripening显示文摘Ethylene plays a pivotal role in climacteric fruit ripening;whereas 1-MCP,a non-toxic antagonist of ethylene,prevents ethylene-dependent responses and fruit ripening.In this study,a short-term treatment(1 h)with 400 nL L^(−1)1-MCP delayed the ripening of harvested papaya.However,long-term application of 1-MCP(400 nL L^(−1),16 h)resulted in abnormal fruit ripening,with the fruits exhibiting normal yellowing without softening,significantly higher cellulose and lignin contents,and intact cell walls(CW).Furthermore,we found that long-term treatment with 1-MCP significantly inhibited the expression of CpEBF1,an EIN3-binding F-box-1 gene.A protein interaction analysis using yeast two-hybrid,BiFC and GST pull-down assays showed that CpEBF1 interacts with the CpMADS1/3 and CpEIL1 proteins.The interaction of CpEBF1 with CpMADS1/3 further activated the activities of CW-degradation gene promoters.Subcellular localization showed that these proteins were localized in the nucleus.Additionally,the expression levels of CpMADS1/3,CpEIL1,and several CW-degradation-related genes were significantly downregulated by long-term 1-MCP treatment.Therefore,we propose that the inhibited expression of CpEBF1 and CpMADS1/3 resulted in the repressed activation of CW-degradation-related genes via their interaction,thereby resulting in fruit softening disorders. | Xiaochun Ding Xiaoyang Zhu Lanlan Ye Shuangling Xiao Zhenxian Wu Weixin Chen Xueping Li | 2019 | Horticulture Research2019，6，1： | 7 |
| 2 | 对比经肛全直肠系膜切除术与腹腔镜全直肠系膜切除术:一项多中心III期随机临床试验(TaLaR试验)方案显示文摘背景:全直肠系膜切除术是直肠癌治疗的标准手术方法。经肛全直肠系膜切除术(taTME)是治疗低位直肠癌的新术式。既往研究表明,与腹腔镜全直肠系膜切除术(lapTME)相比,taTME应用于低位直肠癌患者可提供更高质量的手术标本,但其长期肿瘤学结果仍需进一步观察。因此,我们设计了这个非劣效性临床试验(TaLaR试验),比较taTME与lapTME治疗直肠癌的短期和长期结果。方法/设计:TaLaR试验是一项多中心III期随机对照试验。研究对象为经磁共振成像、直肠指检或结肠镜检查被诊断为位于腹膜反折以下的直肠癌,若肿瘤分期≤cT3N2直接手术,若>cT3N2则先行新辅助治疗。通过计算,本研究需纳入1,114例患者(每组557例)。纳入病例随机分配到taTME或lapTME组。本研究的主要终点是3年无病生存率(DFS)和5年总生存率(OS)。次要终点包括手术标本质量、围手术期结果、骨盆和肛门功能以及生活质量。讨论:我们认为,TaLaR试验将阐明taTME是否可以达到与lapTME相似的肿瘤学结果,以及是否可改善直肠癌患者的手术标本质量及恢复情况。 | Liang Kang Ziwei Zeng Shuangling Luo Hong Zhang Quan Wang Mingyang Ren Miao Wu Weidong Tong Qing Xu Yi Xiao Aiwen Wu Yuan-Guang Chen Bo Feng Zhanlong Shen Liang Huang Xingwei Zhang Minhua Zheng Jian-Ping Wang | 2021 | Gastroenterology Report2021，9，1： | 2 |
| 3 | Studies on adsorp-tion of phenol and 4-nitrophenol on Mg-Al-mixed oxide derivedfrom Mg-Al-layered double hydroxide 显示文摘 | Shuangling Chen Zhi Pingxu Qian Zhang | 2009 | Separation and Purifica-tion Technology2009，67，： | 1 |
| 4 | Lipid Evidence for Oil Depletion by Sulfate-Reducing Bacteria during U Mineralization in the Dongsheng Deposit显示文摘Fatty acids were extracted from fluid inclusions, and analyzed for distribution and individual δ13 C values to determine if there exists sulfate-reducing bacteria(SRB) and what substrates SRB depleted during U mineralization in the Dongsheng deposit. The 10-methylhexadecanoic acid(10 Me16:0) has been detected from most of the samples using GC-MS based on relative retention time and co-injection of standard 10 Me16:0 compound. Samples with higher 10 Me16:0 concentrations, show higher U contents. The presence of 10 Me16:0 along with iso-ω7-cis-heptadecenoic acid(i17:1ω7 c) and ω7-cisoctadecenoic acid(18:1ω7 c) in the fluid inclusions may indicate the occurrence of SRB during the U mineralization. Saturated fatty-acids and unsaturated fatty-acids detected in this study have individual δ13 C values from-30.3‰ to-28.5‰ and-30.5‰ to-27.9‰, respectively. These values are close to those of n-alkanes of the associated oils and the bulk oils, but significantly distinct from methane, thus the oils are concluded to have been used as substrate for the microorganisms to survive on. This proposal is supported by oil biodegradation and ore-stage calcite cement with δ13 C values from-1.4‰ to-17.2‰. | Tiankai Wang Lei Jiang Chunfang Cai Yuyang Yuan Yongdong Zhang Lianqi Jia Shuangling Chen | 2018 | Journal of Earth Science2018，29，3： | 1 |
| 5 | Amylose Content and Starch Granule Size in Rice Grains are Affected By Growing Season显示文摘Amylose content and starch granule size in grains influence rice quality,which differs between the early(ES)and late season(LS).The objective of this study was to determine the variation of amylose content and starch granule size between seasons and find the main reasons(e.g.,temperature and solar radiation)for the observed variation.Field experiments with six rice varieties(three high and three low amylose content rice)planted in the ES and LS were conducted in 2016 and 2017,respectively.The mean temperatures during the filling stage were higher in ES,however,the daily temperatures at 7-10 days after flowering(DAF)in 2016,and at 5-10,13-14 DAF in 2017 were higher in LS.The results showed that amylose content in LS was lower than in ES with high amylose content rice varieties(HACV);the opposite trend occurred with low amylose content rice varieties(LACV).The mean starch granule diameter was higher in LS than ES in 2016,but the opposite result occurred in 2017 with all rice.Our results suggest that higher temperatures increased and decreased the amylose content in HACV and LACV,respectively.Temperatures at 5-15 DAF were important for the formation of starch granules:lower temperatures during 10-14 DAF increased the proportion of larger starch granules(d>6.21μm,some with d>13.3μm),and higher temperatures at 5-6 DAF increased the proportion of starch granules with diameter 4.24-6.21μm. | Hengdong Zhang Min Huang Yingjuan Wei Jiana Chen ShuanglüShan Fangbo Cao Guanghui Chen Yingbin Zou | 2019 | Phyton-International Journal of Experimental Botany2019，88，4： | 0 |
| 6 | The amino acid residue E96 of Ghd8 is crucial for the formation of the flowering repression complex Ghd7-Ghd8-OsHAP5C in rice显示文摘Ghd7 is an important gene involved in the photoperiod flowering pathway in rice.A Ghd7-involved transcriptional regulatory network has been established,but its translational regulatory pathway is poorly understood.The mutant suppressor of overexpression of Ghd7(sog7)was identified from EMS-induced mutagenesis on the background of ZH11 overexpressing Ghd7.Mut Map analysis revealed that SOG7 is allelic to Ghd8 and delayed flowering under long-day(LD)conditions.Biochemical assays showed that Ghd8 interacts with OsHAP5C and Ghd7 both in vivo and in vitro.Surprisingly,a point mutation E96K in theα2 helix of the Ghd8 histone fold domain(HFD)destroyed its ability to interact with Ghd7.The prediction of the structure shows that mutated amino acid is located in the interaction region of CCT/NF-YB/YC complexes,which alter the structure ofα4 of Ghd8.This structural difference prevents the formation of complex NF-YB/YC.The triple complex of Ghd8-OsHAP5C-Ghd7 directly bound to the promotor of Hd3a and downregulated the expression of Ehd1,Hd3a and RFT1,and finally resulted in a delayed heading.These findings are helpful in deeply understanding the Ghd7-involved photoperiod flowering pathway and promote the elucidation of rice heading. | Shuangle Li Yong Hu Chen An Qingli Wen Xiaowei Fan Zhanyi Zhang Ahmed Sherif Haiyang Liu Yongzhong Xing | 2023 | Journal of Integrative Plant Biology2023，65，4： | 0 |
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