|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Transdermal permeation of Zanthoxylum bungeanum essential oil on TCM components with different lipophilicity显示文摘Objective:To investigate the percutaneous penetration enhancement effect of essential oil from Zanthoxylum bungeanum Maxim.(Z.bungeanum oil)on active components in externally-applied traditional Chinese medicines.Methods:Five model drugs,geniposide,puerarin,ferulic acid,tetramethylpyrazine,and osthole,were chosen based on their lipophilicity and tested using in vitro transdermal permeation studies consisting of Franz diffusion cells and full thickness rat abdominal skin.Scanning electron microscopy was employed to observe the morphological changes of rat skin tissue after treatment with Z.bungeanum oil.The molecular interactions between the oil and the polar head groups in stratum corneum(SC)lipids were monitored using molecular dynamic simulation,and the SC/vehicle partition coefficients and saturation solubilities of the selected model drugs treated with and without the oil were also determined to ascertain its mechanisms of action.Results:As oil concentration increased,the log ERflow trended toward a negative linear relationship with the lipophilicity of drugs.After treatment with Z.bungeanum oil,a mild lifting up and wrinkle on the SC surface were observed,and appeared to become more pronounced as oil concentration increased.There was no significant difference between the control and the Z.bungeanum oil at different concentrations in terms of saturation solubility of GP,while saturation solubilities of the 4 other drugs gradually increased as oil concentration increased.The oxygen-containing constituents in Z.bungeanum oil,such as terpinen-4-ol and 1,8-cineole,which accounted for 57.95%of total oil,could form stable hydrogen bonds with the polar head group of ceramide 3.Conclusion:Z.bungeanum oil facilitated transdermal permeation of drugs with different lipophilicity,including the extremely hydrophilic and lipophilic drugs,whereas it exhibited greater enhancement activity for strongly hydrophilic drugs.The mechanisms of transdermal permeation enhancement by the oil could be explained with SC/vehicle partition coefficient,saturation solubility,and the interactions with SC lipids. | Yi Lan Jingyan Wang Shuwang He Shuying Li Peng Tan Hongmei Lin Qing Wu | 2016 | Journal of Traditional Chinese Medical Sciences2016，3，3： | 6 |
| 2 | Quantitative prediction of the bitterness of atomoxetine hydrochloride and taste-masked using hydroxypropyl-β-cyclodextrin:A biosensor evaluation and interaction study显示文摘The bitterness of a drug is a major challenge for patient acceptability and compliance,especially for children.Due to the toxicity of medication,a human taste panel test has certain limitations.Atomoxetine hydrochloride(HCl),which is used for the treatment of attention deficit/hyperactivity disorder(ADHD),has an extremely bitter taste.The aim of this work is to quantitatively predict the bitterness of atomoxetine HCl by a biosensor system.Based on the mechanism of detection of the electronic tongue(Etongue),the bitterness of atomoxetine HCl was evaluated,and it was found that its bitterness was similar to that of quinine HCl.The bitterness threshold of atomoxetine HCl was 8.61μg/ml based on the Change of membrane Potential caused by Adsorption(CPA)value of the BT0 sensor.In this study,the taste-masking efficiency of 2-hydroxypropyl-β-cyclodextrin(HP-β-CyD)was assessed by Euclidean distances on a principle component analysis(PCA)map with the SA402B Taste Sensing System,and the host–guest interactions were investigated by differential scanning calorimetry(DSC),powder X-ray diffraction(XRD),nuclear magnetic resonance(NMR)spectroscopy and scanning electron microscopy(SEM).Biosensor evaluation and characterization of the inclusion complex indicated that atomoxetine HCl could actively react with 2-hydroxypropyl-β-cyclodextrin. | Shuying Li Ying Zhang Abdur Rauf Khan Shuwang He Yingxin Wang Jiangkang Xu Guangxi Zhai | 2020 | Asian Journal of Pharmaceutical Sciences2020，15，4： | 2 |
| 3 | Study of 'Wave Type' uneven settlement and reinforcing method of soft clay foundation 显示文摘 | Sun Liqiang Yan Shuwang He Hongjuan | 2011 | Advanced Materials Re- search2011，，： | 1 |
| 4 | Renin--angiotensin system inhibitor is associated with the reduced risk of all-cause mortality in COVID-19 among patients with/without hypertension显示文摘Consecutively hospitalized patients with confirmed coronavirus disease 2019(COVID-19)in Wuhan,China were retrospectively enrolled from January 2020 to March 2020 to investigate the association between the use of renin–angiotensin system inhibitor(RAS-I)and the outcome of this disease.Associations between the use of RAS-I(angiotensin-converting enzyme inhibitor(ACEI)or angiotensin receptor blocker(ARB)),ACEI,and ARB and in-hospital mortality were analyzed using multivariate Cox proportional hazards regression models in overall and subgroup of hypertension status.A total of 2771 patients with COVID-19 were included,with moderate and severe cases accounting for 45.0%and 36.5%,respectively.A total of 195(7.0%)patients died.RAS-I(hazard ratio(HR)=0.499,95%confidence interval(CI)0.325–0.767)and ARB(HR=0.410,95%CI 0.240–0.700)use was associated with a reduced risk of all-cause mortality among patients with COVID-19.For patients with hypertension,RAS-I and ARB applications were also associated with a reduced risk of mortality with HR of 0.352(95%CI 0.162–0.764)and 0.279(95%CI 0.115–0.677),respectively.RAS-I exhibited protective effects on the survival outcome of COVID-19.ARB use was associated with a reduced risk of all-cause mortality among patients with COVID-19. | Huai-yu Wang Suyuan Peng Zhanghui Ye Pengfei Li Qing Li Xuanyu Shi Rui Zeng Ying Yao Fan He Junhua Li Liu Liu Shuwang Ge Xianjun Ke Zhibin Zhou Gang Xu Ming-hui Zhao Haibo Wang Luxia Zhang Erdan Dong | 2022 | Frontiers of Medicine2022，16，1： | 0 |
| 5 | Spreading fully at the ice-water interface is required for high ice recrystallization inhibition activity显示文摘Although materials for ice recrystallization inhibition(IRI) are essential for the cryopreservation of cells and tissues, there exists no guiding mechanism for the design of such materials. Therefore, the construction of materials for IRI relies on the try-and-error strategy. Herein, through changing the tacticity of hydroxyl groups on poly(vinyl alcohol)(PVA) backbones with the affinities of PVAs to ice unchanged, we experimentally find IRI activity decreases significantly for isotactic PVA in comparison to that of atactic PVA. Molecular dynamics simulation shows atactic PVA spreads fully at the ice-water interface due to its much stronger interaction with water. This indicates atactic PVA can cover more ice surface and possess a higher IRI activity when the same amount of PVAs are used, which is consistent with the results that PVA can cover the same amount of ice surface more efficiently through experimentally measuring the adsorption of PVAs on the ice surface. A guiding mechanism of high active IRI materials can be obtained: only having affinity to ice is not enough to obtain high IRI activity(i.e., only small amount of materials is required to reduce the size of ice crystals to ca.(35±10)μm), IRI agents must also have high affinity to water, i.e., low interfacial energies, to both ice and water. The former is to guarantee the adsorption of the IRI agent on the ice surface, and the latter is required for the IRI agent to spread sufficiently at the ice-water interface. Therefore, each IRI molecule can effectively block the diffusion of water onto the ice surface, and consequently inhibits the growth of ice. A spreading coefficient of IRI agents is therefore introduced to quantitatively assess the capability of IRI agents to spread at the ice-water interface. | Shenglin Jin Lingkang Yin Bin Kong Shuwang Wu Zhiyuan He Han Xue Zhang Liu Qi Cheng Xin Zhou Jianjun Wang | 2019 | Science China Chemistry2019，62，7： | 0 |
| 6 | Differential protein expression in rat cortical astrocytes following fluid percussion injury Two-dimensional gel electrophoresis and mass-spectrum detection显示文摘BACKGROUND: The Glasgow Coma Scale,computer tomography,and nuclear magnetic reso-nance imaging have been frequently used to diagnose brain injury.However,these methods do not accurately and quantitatively evaluate injury degree.However,proteomics displays some advan-tages.To date,there are few proteomics studies based on primary astrocyte cultures from a fluid percussion injury model.OBJECTIVE: To detect differential protein expression in rat cerebral cortical astrocytes following fluid percussion injury using two-dimensional gel electrophoresis and mass spectrum and to deter-mine specific biological markers of brain injury.DESIGN,TIME AND SETTING: Complete,randomized grouping and proteomics experiments were performed at the Molecular Pathological Laboratory,Central Laboratory and Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard of Medical College of Chinese People's Armed Police Force from October 2007 to May 2008.MATERIALS: Inverted phase-contrast microscope was purchased from Olympus,Japan.PRO-TEAN IEF Cell isoelectric focusing electrophoresis system and PROTEAN II Xi-Cell vertical elec-trophoresis system were purchased from Bio-Rad,USA.Autoflex MALDI-TOF mass spectrometer was purchased from Bruker,Germany.METHODS: A total of 90 culture dishes,fully coated with Sprague Dawley rat cortical astrocytes,were randomly divided into control (n = 30) and injury (n = 60) groups.Astrocytes in the injury group were subjected to fluid percussion and subdivided into 4-hour (n = 30) and 48-hour injury (n = 30) groups.MAIN OUTCOME MEASURES: Cell morphology was observed using inverted phase-contrast mi-croscopy.Cell total protein was extracted from each group,followed by two-dimensional gel elec-trophoresis and silver staining,and the differential protein expression was analyzed using PDQuest 7.0 software.Protein peptide mass fingerprinting of differential protein spots was obtained by matrix assisted laser desorption/ionization-time of flight mass spectrometry.The National Center for Bio-technology Information (NCBI) protein database was retrieved by Mascot to primarily identify protein type.Finally,differential protein expression was detected by Western blot analysis.RESULTS: Following fluid percussion injury,astrocytes displayed obvious swelling and increased intercellular space,with some cell detachment;the number of dead cells was significantly greater than the control group (P < 0.05).Expression intensity of 114 protein spots was significantly greater in the injury group compared with the control group (P < 0.05);9 of the 114 protein spots were iden-tified and peptide matching scores of 8 spots were > 61 (P < 0.05).Protein types were identified and included cellular retinol binding protein,brain fatty acid binding protein 7,S100 calcium binding protein A11,60S acidic ribosomal protein P2,calponin 3,breast carcinoma amplified sequence 2 homolog,eukaryotic translation initiation factor 1A,and hypothetical protein LOC685814.Western blot detection revealed brain fatty acid binding protein 7 expression in cortical astrocytes,which in-creased with injury time compared with the control group (P < 0.05).CONCLUSION: Results from this study showed morphological and proteomic changes in cortical astrocytes following fluid percussion injury.Brain fatty acid binding protein 7 was expressed in as-trocytes and possibly played an important role in injury repair.Mass-spectrum identified differentially expressed proteins that correlated with cell metabolism regulation,signal transduction,and transla-tion initiation,and could serve as specific biological markers of brain injury. | Yongliang Zhang Lingzhi Li Baoguo Yu Xiaojing Qin Shuwang Yang Tao He | 2010 | Neural Regeneration Research2010，5，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员