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| 1 | Pro-apoptotic and anti-proliferative effects of Physalis angulata leaf extract on retinoblastoma cells显示文摘AIM: To investigate the effect of Physalis angulata leaf extract on apoptotic and proliferation of retinoblastoma cells. Despite several previous studies evidencing the anticancer potential of Physalis angulata;however, certain study that proves its benefits in retinoblastoma cancer cells has been limited.METHODS: This study utilizes an in-vitro experimental study by applying Y79 human retinoblastoma cell line culture obtained from the American Type Culture Collection(ATCC;10801 University Boulevard Manassas, VA 20110, USA). The cell was divided into 4 groups. Group I was the control group without the administration of Physalis angulata leaf extract. Whereas, group II, II and IV are engaged with 25, 50, and 100 μg/mL of Physalis angulata leaf extract respectively. After a 24 h incubation, an examination with microtetrazolium(MTT) cell proliferation assay and Annexin V apoptosis detection was conducted. Statistical analysis was performed with the Tukey test.RESULTS: Physalis angulata leaf extract improved apoptosis and significantly reduced the number of living cells in retinoblastoma cells, along with the increase in the given dose. Based on the Tukey test, a significant difference was found in the treatment group at 50 μg/mL(P=0.025) and 100 μg/mL(P=0.001) in the measurement of apoptosis. Proliferation measurements also indicated a significant decrease in the number of living cells in the 50μg/m L treatment group(P=0.004), and in the 100 μg/mL treatment group(P=0.000). Meanwhile, a dose of 25 μg/mL indicated insignificant difference in the two measurements. Improved apoptosis and decreased number of living cells occured at a dose of 100 μg/mL. Decreased number of living cells(in the measurement of proliferation) was due to the inhibited proliferation or improved apoptosis.CONCLUSION: Physalis angulata leaf extract improve apoptosis in retinoblastoma cell culture, requiring further research to inhibit proliferation. | Marsha Dechastra Chairissy Lely Retno Wulandari Hidayat Sujuti | 2019 | International Journal of Ophthalmology(English edition)2019，12，9： | 3 |
| 2 | Raptor, a Binding Partner of Target of Rapamycin (TOR), Mediates TOR Action显示文摘 | Kenta Hara Yoshiko Maruki Xiaomeng Long Ken-ichi Yoshino Noriko Oshiro Sujuti Hidayat Chiharu Tokunaga Joseph Avruch Kazuyoshi Yonezawa | 2002 | Cell2002，，2： | 1 |
| 3 | 谷胱甘肽和烟酸对高糖状态下人晶状体上皮细胞的影响(英文)显示文摘目的:评价还原性谷胱甘肽(GSH)和烟酸联合应用对蛋白质氧化应激、内质网应激(ER)、糖基化和聚合αβ晶状体蛋白的人晶状体上皮(HLE)细胞治疗高葡萄糖水平的影响。方法:培养HLE细胞并暴露于25 mmol/L葡萄糖中以促进高糖环境。各组细胞用三种不同的剂量组合进行联合治疗:10μmol/L GSH+2μmol/L烟酸,30μmol/L GSH+25μmol/L烟酸,100μmol/L GSH+25μmol/L烟酸。培养72h后,用ELISA法测定蛋白羰基(PCC)和葡萄糖反应蛋白(GRP78)的含量。培养2wk后,对糖基化终末产物(AGEs)进行检测,用Western Blot检测αβ晶状体蛋白的表达。所有数据分析均采用SPSS 18.0统计软件包。结果:与对照组相比,联合治疗组的PCC和GRP78水平没有显著降低(P>0.05)。相反,与对照组相比,GSH和烟酸联合治疗组的AGEs水平明显下降(P<0.05)。此外,高剂量葡萄糖给药后αβ晶状体蛋白表达增加,但GSH联合治疗组与GSH和烟酸联合治疗组αβ晶状体蛋白表达都有所降低。结论:结果表明,GSH和烟酸联合应用可抑制高血糖人HLE细胞蛋白质的聚合,防止糖基化,并且通过AGEs途径在预防糖尿病性白内障中发挥积极作用。 | Nina Handayani Nur Permatasari Hidayat Sujuti Achmad Rudijanto | 2020 | 国际眼科杂志2020，20，1： | 1 |
| 4 | Mechanism of retinal pericyte migration through Angiopoietin/Tie-2 signaling pathway on diabetic rats显示文摘AIM: To investigate the mechanism of pericyte migration through Angiopoietin-2(Ang-2)/Tie-2 signaling pathway.METHODS: We divided the rats into 5 groups. Each diabetic rat model groups injected with Tie-2 inhibitor, ERK1/2 inhibitor, Akt/PKB inhibitor, and DMSO intravitreal. Retinal digest preparation was done to examine the retinal vasculature including pericyte: endothelial ratio, and morphology of pericyte migration. Tie-2, ERK1/2 and Akt/PKB phosporylation were analyzed by confocal laser scanning microscopy.RESULTS: There was a correlation between pericyte migration with increasing Ang-2(P<0.05). Pericyte number reduced by 40%(1:2.4) after 5 wk diabetes on diabetic rats. The pericyte: endothelial ratio on group with Tie-2 inhibitor were 1:1.8. The same result shows on group with Akt/PKB inhibition. ERK1/2 inhibitor group shows the best results of pericyte: endothelial ratio(1:1.7). Inhibition on Tie-2 receptor decreased the phosphorylation activity of Tie-2, ERK1/2 and Akt/PKB pathway. ERK1/2 inhibition also decreasing the phosphorylation of Tie-2 and Akt/PKB. But on Akt/PKB inhibition, the phosphorylation of Tie-2 and ERK1/2 were relative the same.CONCLUSION: Ang-2 has a role for pericyte migration on diabetic rats through Tie-2 receptor, ERK1/2 and Akt/PKB pathways. ERK1/2 is a dominant pathway based on theability to supress another pathway activity and decreasing pericyte migration on diabetic rats. | Nadia Artha Dewi Aulanni'am Aulanni 'am Hidayat Sujuti Muhammad Aris Widodo Ojoko Wahono Soeatmadji | 2018 | International Journal of Ophthalmology(English edition)2018，11，3： | 1 |
| 5 | Neuropathy optic glaucomatosa induced by systemic hypertension through activation endothelin-1 signaling pathway in central retinal artery in rats显示文摘· AIM: To evaluate effect of hypertension on retinal ganglion cell(RGC) apoptosis, intraocular pressure(IOP),and the activation of endothelin-1(ET-1) signaling pathway in central retinal artery(CRA) in rats.·METHODS: The experimental study was performed on20 male Sprague Dawley rats that were divided into control group, and hypertension groups. The hypertension was induced by subcutaneous deoxycorticoacetate(DOCA)10 mg/kg twice a week and administered 0.9% Na Cl solution daily for 2, 6, and 10 wk. Blood pressure(BP) was measured using animal BP analyzer. IOP was measured by handheld tonometry. Retinal tissue preparations by paraffin blocks were made after enucleation. The expression of ET-1, e NOS, ET-1 receptor A(ETRA), ET-1receptor B(ETRB), and phosphorylated myosin light chain kinase(MLCK), and caldesmon(Ca D) in CRA and RGC apoptosis were evaluated through immunofluorescent staining method then observed using laser scanning confocal microscopy.· RESULTS: BP significantly increased in all of the hypertension groups compared to control(P =0.001).Peak IOP elevation(7.78±4.14 mm Hg) and RGC apoptosis(576.15±33.28 Au) occurred on 2wk of hypertension. ET-1expression(1238.6±55.1 Au) and e NOS expression(2814.2±70.7 Au) were found highest in 2wk of hypertension,although the ratio of ET-1/e NOS decreased since 2wk.ETRAreached peak expression in 10 wk of hypertension(1219.4 ±6.3 Au), while ETRBsignificantly increased only in 2 weeks group(1069.2 ±9.6 Au). The highest MLCK expression(1190.09±58.32 Au), Ca D(1670.28±18.36 Au)were also found in 2wk of hypertension.·CONCLUSION: Hypertension effects to activation of ET-1 signaling pathway significantly in CRA, elevation of IOP, and RGC apoptosis. The highest value was achieved at 2wk, which is the development phase of hypertension. | Seskoati Prayitnaningsih Hidayat Sujuti Maksum Effendi Aulia Abdullah Nanda Wahyu Anandita Febriani Yohana Nur Permatasari Mohamad Aris Widodo | 2016 | International Journal of Ophthalmology(English edition)2016，9，11： | 1 |
| 6 | Raptor, a Binding Partner of Target of Rapamycin (TOR), Mediates TOR Action显示文摘 | Kenta Hara Yoshiko Maruki Xiaomeng Long Ken-ichi Yoshino Noriko Oshiro Sujuti Hidayat Chiharu Tokunaga Joseph Avruch Kazuyoshi Yonezawa | 2002 | Cell2002，，2： | 1 |
| 7 | Different levels of IL-1 α,IL-6,TNF-α,NF-κB and PPAR-γin monocyte cultures exposed by plasma preeclampsia and normotensive pregnancy显示文摘 | Rahardjo B Widjajanto E Sujuti H | 2014 | Pregnancy Hypertens2014，4，3： | 1 |
| 8 | Effect of epigallocatechin gallate in green tea on preventing lens opacity and αB-crystallin aggregation in rat model of diabetes显示文摘AIM:To evaluate the effect of epigallocatechin gallate(EGCG) in preventing lens opacity and the aggregation of lens αB-crystallin in model rats of diabetes mellitus(DM).METHODS:This experimental study included Wistar rats for DM as in vivo models and divided into 5 groups.The treatment groups were administered EGCG by orally for 20d and were then assessed for their degree of lens opacity with binocular microscope and lens αB-crystallin expression from Western blot analyze.RESULTS:Pearson correlation test and regression analysis on EGCG exposure and final random blood sugar(RBS) obtained a significance level of P<0.05.EGCG exposure can significantly lower RBS with an R~2 of 0.5634(56.34%).The same analysis on EGCG exposure and the degree of lens opacity obtained a significance level of P<0.05 and increased exposure to EGCG can significantly lower the degree of lens opacity with an R~2 of 0.8577(85.77%).Correlation analysis between EGCG and the expression of lens αB-crystallin can be concluded that the higher the EGCG exposure administered,the higher the native lens αB-crystallin expression and the lower the aggregate lens αB-crystallin expression.There was also significant effect in which every 1 mg/kg body weight dose of EGCG can increase the native lens αB-crystallin expression by 0.0063 and decrease the aggregate lens αB-crystallin expression by 0.0076.CONCLUSION:The administration of EGCG at a dose of 300,600,and 1200 mg shows a significant effect on preventing lens opacity and aggregation of αB-crystallin in diabetic rat models and this research could be a biomolecular prevention of cataract. | Andita Gustria Caesary Nina Handayani Hidayat Sujuti | 2023 | International Journal of Ophthalmology(English edition)2023，16，3： | 0 |
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