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| 1 | Transplantation of UC-MSCs on collagen scaffold activates follicles in dormant ovaries of POF patients with long history of infertility显示文摘Premature ovarian failure(POF) is a refractory disease for clinical treatment with the goal of restoring fertility. In this study,umbilical cord mesenchymal stem cells on a collagen scaffold(collagen/UC-MSCs) can activate primordial follicles in vitro via phosphorylation of FOXO3 a and FOXO1. Transplantation of collagen/UC-MSCs to the ovaries of POF patients rescued overall ovarian function, evidenced by elevated estradiol concentrations, improved follicular development, and increased number of antral follicles. Successful clinical pregnancy was achieved in women with POF after transplantation of collagen/UC-MSCs or UC-MSCs. In summary, collagen/UC-MSC transplantation may provide an effective treatment for POF. | Lijun Ding Guijun Yan Bin Wang Lu Xu Yan Gu Tong Ru Xiaoying Cui Lei Lei Jingyu Liu Xiaoqiang Sheng Bin Wang Chunxue Zhang Yanjun Yang Ruiwei Jiang Jianjun Zhou Na Kong Feifei Lu Huaijun Zhou Yannan Zhao Bing Chen Yali Hu Jianwu Dai Haixiang Sun | 2018 | Science China(Life Sciences)2018，61，12： | 43 |
| 2 | Transplantation of collagen scaffold with autologous bone marrow mononuclear cells promotes functional endometrium reconstruction via downregulating ΔNp63 expression in Asherman's syndrome显示文摘Asherman's syndrome(AS) is a common disease that presents endometrial regeneration disorder. However, little is known about its molecular features of this aregenerative endometrium in AS and how to reconstruct the functioning endometrium for the patients with AS. Here, we report that ΔNp63 is significantly upregulated in residual epithelial cells of the impaired endometrium in AS; the upregulated-ΔNp63 induces endometrial quiescence and alteration of stemness. Importantly, we demonstrate that engrafting high density of autologous bone marrow mononuclear cells(BMNCs) loaded in collagen scaffold onto the uterine lining of patients with AS downregulates ΔNp63 expression, reverses ΔNp63-induced pathological changes, normalizes the stemness alterations and restores endometrial regeneration. Finally, five patients achieved successful pregnancies and live births. Therefore, we conclude that ΔNp63 is a crucial therapeutic target for AS. This novel treatment significantly improves the outcome for the patients with severe AS. | Guangfeng Zhao Yun Cao Xianghong Zhu Xiaoqiu Tang Lijun Ding Haixiang Sun Juan Li Xinan Li Chenyan Dai Tong Ru Hui Zhu Jingjie Lu Caimei Lin Jingmei Wang Guijun Yan Huiyan Wang Lei Wang Yimin Dai Bin Wang Ruotian Li Jianwu Dai Yan Zhou Yali Hu | 2017 | Science China(Life Sciences)2017，60，4： | 39 |
| 3 | Collagen scaffolds modified with collagen-binding bFGF promotes the neural regeneration in a rat hemisected spinal cord injury model显示文摘Nerve conduit is one of strategies for spine cord injury(SCI)treatment.Recently,studies showed that biomaterials could guide the neurite growth and promote axon regeneration at the injury site.However,the scaffold by itself was difficult to meet the need of SCI functional recovery.The basic fibroblast growth factor(bFGF)administration significantly promotes functional recovery after organ injuries.Here,using a rat model of T9 hemisected SCI,we aimed at assessing the repair capacity of implantation of collagen scaffold(CS)modified by collagen binding bFGF(CBD-bFGF).The results showed that CS combined with CBD-bFGF treatment improved survival rates after the lateral hemisection SCI.The CS/CBD-bFGF group showed more significant improvements in motor than the simply CS-implanted and untreated control group,when evaluated by the 21-point Basso-Beattie-Bresnahan(BBB)score and footprint analysis.Both hematoxylin and eosin(H&E)and immunohistochemical staining of neurofilament(NF)and glial fibrillary acidic protein(GFAP)demonstrated that fibers were guided to grow through the implants.These findings indicated that administration of CS modified with CBD-bFGF could promote spinal cord regeneration and functional recovery. | SHI Qin GAO Wei HAN XingLong ZHU XueSong SUN Jie XIE Fang HOU XiangLin YANG HuiLin DAI JianWu CHEN Liang | 2014 | Science China(Life Sciences)2014，57，2： | 23 |
| 4 | 2016 Chinese expert consensus and guidelines for the diagnosis and treatment of papillary thyroid microcarcinoma显示文摘The incidence of papillary thyroid carcinoma(PTC)has exponentially increased in recent years.Papillary thyroid microcarcinoma(PTMC)accounts for the majority of the reported cases of PTC.The debates and crucial issues in PTMC management have received researchers'attention.To further improve the clinical management of PTMC in China, | Ming Gao Minghua Ge Qinghai Ji Ruochuan Cheng Hankui Lu Haixia Guan Li Gao Zhuming Guo Tao Huang Xiaoming Huang Xiaoming Li Yansong Lin Qinjiang Liu Xin Ni Yi Pan Jianwu Qin Zhongyan Shan Hui Sun Xudong Wang Zhengang Xu Yang Yu Daiwei Zhao Naisong Zhang ShengZhang Ying Zheng Jingqiang Zhu Dapeng Li Xiangqian Zheng 无 | 2017 | Cancer Biology & Medicine2017，14，3： | 23 |
| 5 | Eddy covariance measurements of water vapor and CO_2 fluxes above the Erhai Lake显示文摘Measurement of turbulence fluxes were performed over the Erhai Lake using eddy covariance(EC) method.Basic physical parameters in the lake-air interaction processes,such as surface albedo of the lake,aerodynamic roughness length,bulk transfer coefficients,etc.,were investigated using the EC data in 2012.The characteristics of turbulence fluxes over the lake including momentum flux,sensible heat flux,latent heat flux,and CO2 flux,and their controlling factors were analyzed.The total annual evaporation of the lake was also estimated based on the artificial neural network(ANN) gap-filling technique.Results showed that the total annual evaporation in 2012 was 1165 ± 15 mm,which was larger than the annual precipitation(818 mm).Local circulation between the lake and the surrounding land was found to be significant throughout the year due to the land-lake breeze or the mountain-valley breeze in this area.The prevailing winds of southeasterly and northwesterly were observed throughout the year.The sensible heat flux over this plateau lake usually had a few tens of W m-2,and generally became negative in the afternoon,indicating that heat was transferred from the lake to the atmosphere.The sensible heat flux was governed by the lake-air temperature difference and had its maximum in the early morning.The diurnal variation of the latent heat flux was controlled by vapor pressure deficit with a peak in the afternoon.The latent heat flux was dominant in the partition of available energy in daytime over this lake.The lake acted as a weak CO2 source to the atmosphere except for the midday of summer.Seasonal variations of surface albedo over the lake were related to the solar elevation angle and opacity of the water.Furthermore,compared with the observation data,the surface albedo estimated by CLM4-LISSS model was underestimated in winter and overestimated in summer. | LIU HuiZhi FENG JianWu SUN JiHua WANG Lei XU AnLun | 2015 | Science China Earth Sciences2015，58，3： | 15 |
| 6 | OPTIMAL CONTROL APPLIED IN AUTOMATIC CLUTCH ENGAGEMENTS OF VEHICLES显示文摘Start-up working condition is the key to the research of optimal engagement of automatic clutch for AMT.In order to guarantee an ideal dynamic performance of the clutch engagement,an optimal controller is designed by considering throttle angle,engine speed,gear ratio,vehicle acceleration and road condition.The minimum value principle is also introduced to achieve an optimal dynamic performance of the nonlinear system compromised in friction plate wear and vehicle drive quality.The optimal trajectory of the clutch engagement can be described in the form of explicit and analytical expressions and characterized by the deterministic and accurate control strategy in stead of indeterministic and soft control techniques which need thousands of experiments.For validation of the controller,test work is carried out for the automated clutch engagements in a commercial car with an traditional mechanical transmission,a hydraulic actuator,a group of sensors and a portable computer system.It is shown through experiments that dynamic behaviors of the clutch engagement operated by the optimal control are more effective and efficient than those by fuzzy control. | Sun Chengshun Zhang Jianwu School of Mechanical and Power Engineering,Shanghai Jiaotong University,Shanghai 200030, China | 2004 | Chinese Journal of Mechanical Engineering2004，17，2： | 13 |
| 7 | Coastal blue carbon: Concept, study method, and the application to ecological restoration显示文摘Coastal blue carbon refers to the carbon taken from atmospheric CO2; fixed by advanced plants(including salt marsh,mangrove, and seagrass), phytoplankton, macroalgae, and marine calcifiers via the interaction of plants and microbes; and stored in nearshore sediments and soils; as well as the carbon transported from the coast to the ocean and ocean floor. The carbon sequestration capacity per unit area of coastal blue carbon is far greater than that of the terrestrial carbon pool. The mechanisms and controls of the carbon sink from salt marshes, mangroves, seagrasses, the aquaculture of shellfish and macroalgae, and the microbial carbon pump need to be further studied. The methods to quantify coastal blue carbon include carbon flux measurements, carbon pool measurements, manipulative experiments, and modeling. Restoring, conserving, and enhancing blue carbon will increase carbon sinks and produce carbon credits, which could be traded on the carbon market. The need to tackle climate change and implement China's commitment to cut carbon emissions requires us to improve studies on coastal blue carbon science and policy. The knowledge learned from coastal blue carbon improves the conservation and restoration of salt marshes,mangroves, and seagrasses; enhances the function of the microbial carbon pump; and promotes sustainable aquaculture, such as ocean ranching. | Jianwu TANG Shufeng YE Xuechu CHEN Hualei YANG Xiaohong SUN Faming WANG Quan WEN Shaobo CHEN | 2018 | Science China Earth Sciences2018，61，6： | 8 |
| 8 | 2017 Chinese expert consensus on the clinical application ofserum marker for thyroid cancer显示文摘In recent years, the clinical incidence of thyroid cancer has been increasing year by year, and its risk assessment and clinical management methods have also been accordingly modified and constantly improved. There are great differences between the clinical diagnostic and therapeutic modes and disease management of thyroid cancer employed by various medical institutions in China, particularly with regard to the clinical application of serum marker of thyroid cancer. To this end, the China Anti-Cancer Association Thyroid Cancer Specialized Committee Chinese Association of Thyroid Oncology organized this compilation of ExpertConsensus on Clinical Application of Serum Marker of Thyroid Cancer to help and impel relevant clinical institutions and professionals to standardize clinical diagnosis, treatment, and long-term management of thyroid cancer, and to properly utilize the serum marker for scientific auxiliary clinical diagnosis and assessment of thyroid cancer before and after operation. | Ming Gao Minghua Ge Qinghai Ji Ruochuan Cheng Hankui Lu Haixia Guan Wei Cui Li Gao Zairong Gao Lin Guo Zhuming Guo Tao Huang Xiaoming Huang Yansong Lin Qinjiang Liu Xin Ni Jianwu Qin Li Ren Zhongyan Shan Hui Sun Xudong Wang Zhengang Xu Yang Yu Bin Zhang DaiweiZhao Ying Zheng Jingqiang Zhu Xiangqian Zheng 无 | 2018 | Cancer Biology & Medicine2018，15，4： | 3 |
| 9 | Single-cell RNA sequencing reveals Nestin+active neural stem cells outside the central canal after spinal cord injury显示文摘Neural stem cells(NSCs)in the spinal cord hold great potential for repair after spinal cord injury(SCI).The ependyma in the central canal(CC)region has been considered as the NSCs source in the spinal cord.However,the ependyma function as NSCs after SCI is still under debate.We used Nestin as a marker to isolate potential NSCs and their immediate progeny,and characterized the cells before and after SCI by single-cell RNA-sequencing(scRNA-seq).We identified two subgroups of NSCs:the subgroup located within the CC cannot prime to active NSCs after SCI,while the subgroup located outside the CC were activated and exhibited the active NSCs properties after SCI.We demonstrated the comprehensive dynamic transcriptome of NSCs from quiescent to active NSCs after SCI.This study reveals that Nestin+cells outside CC were NSCs that activated upon SCI and may thus serve as endogenous NSCs for regenerative treatment of SCI in the future. | Muya Shu Xiaoyu Xue Hu Nie Xianming Wu Minghan Sun Lianyong Qiao Xing Li Bai Xu Zhifeng Xiao Yannan Zhao Yongheng Fan Bing Chen Jixiang Zhang Ya Shi Yaming Yang Falong Lu Jianwu Dai | 2022 | Science China(Life Sciences)2022，65，2： | 2 |
| 10 | Microvascular endothelial cells derived from spinal cord promote spinal cord injury repair显示文摘Neural regeneration after spinal cord injury (SCI) closely relates to the microvascular endothelial cell (MEC)- mediated neurovascular unit formation. However, the effects of central nerve system-derived MECs on neovascularization and neurogenesis, and potential signaling involved therein, are unclear. Here, we established a primary spinal cord-derived MECs (SCMECs) isolation with high cell yield and purity to describe the differences with brain-derived MECs (BMECs) and their therapeutic effects on SCI. Transcriptomics and proteomics revealed differentially expressed genes and proteins in SCMECs were involved in angiogenesis, immunity, metabolism, and cell adhesion molecular signaling was the only signaling pathway enriched of top 10 in differentially expressed genes and proteins KEGG analysis. SCMECs and BMECs could be induced angiogenesis by different stiffness stimulation of PEG hydrogels with elastic modulus 50-1650 Pa for SCMECs and 50-300 Pa for BMECs, respectively. Moreover, SCMECs and BMECs promoted spinal cord or brain-derived NSC (SNSC/BNSC) proliferation, migration, and differentiation at different levels. At certain dose, SCMECs in combination with the NeuroRegen scaffold, showed higher effectiveness in the promotion of vascular reconstruction. The potential underlying mechanism of this phenomenon may through VEGF/AKT/eNOS- signaling pathway, and consequently accelerated neuronal regeneration and functional recovery of SCI rats compared to BMECs. Our findings suggested a promising role of SCMECs in restoring vascularization and neural regeneration. | Zhifeng You Xu Gao Xinyi Kang Wen Yang Tiandi Xiong Yue Li Feng Wei Yan Zhuang Ting Zhang Yifu Sun He Shen Jianwu Dai | 2023 | Bioactive Materials2023，，11： | 2 |
| 11 | Recent developments in regenerative ophthalmology显示文摘Regenerative medicine(RM)is one of the most promising disciplines for advancements in modern medicine,and regenerative ophthalmology(RO)is one of the most active fields of regenerative medicine.This review aims to provide an overview of regenerative ophthalmology,including the range of tools and materials being used,and to describe its application in ophthalmologic subspecialties,with the exception of surgical implantation of artificial tissues or organs(e.g.,contact lens,artificial cornea,intraocular lens,artificial retina,and bionic eyes)due to space limitations.In addition,current challenges and limitations of regenerative ophthalmology are discussed and future directions are highlighted. | Ye Shen He Shen Dongyu Guo Xinghuai Sun Yuan Sun Nan Hong Xiawei Wang Chen Xie Yuan Zhao Qin He Le Jin Yingying Wen Bo Jiang Chenying Yu Miaomiao Zhu Feng Cai Jianwu Dai | 2020 | Science China(Life Sciences)2020，63，10： | 2 |
| 12 | Influence of improper installation on measurement performance of pitot tube显示文摘 | Sun Zhiqiang Li Zhiyong Zheng Jianwu | 2009 | International Conference on Industrial Mechatronics and Automation2009，，： | 1 |
| 13 | Hole transport in p-type ZnO films grown by plasma-assisted molecular beam epitaxy显示文摘 | Sun Jianwu Lu Youming Liu Yichun | 2006 | Applied Physics Letters2006，89，23： | 1 |
| 14 | Targeting Annexin A7 in Hepatocarcinoma Lymphatic Metastasis显示文摘Background and objective The rst aim was to measure the expressions of Annexin A7 (Anxa7) at mRNA level and protein level in two mice hepatocarcinoma ascites syngeneic cell | Mmgzhong SUN Shuqing LIU Jianwu TANG | 2009 | 中国肺癌杂志2009，12，6： | 1 |
| 15 | Expression of Bacillus thuringiensis full-length and N terminally truncated vip184 gene in an acrystalliferous strain of subspecies kurstaki 显示文摘 | Chen Jianwu Sun Fan Tang Lixia | 2003 | World Journal of Microbiology & Biotechnology2003，19，： | 1 |
| 16 | Further properties on the composition growth of a class of meromorphic functions 显示文摘 | Jianwu Sun | 2003 | Complex Variables Theory and Application an International Journal2003，48，2： | 1 |
| 17 | Controllable synthesis of monodispersed silver nanoparticles as standards for quantitative assessment of their cytotoxicity显示文摘 | Lun Li Jie Sun Xiaoran Li Yan Zhang Zhaoxu Wang Chunren Wang Jianwu Dai Qiangbin Wang | 2011 | Biomaterials2011，，6： | 1 |
| 18 | Mannose selection system used for cucumber transformation显示文摘 | Zhengquan He ZhenZhen Duan Wei Liang Faju Chen Wei Yao Hongwei Liang Chaoyin Yue Zongxiu Sun Fan Chen Jianwu Dai | 2006 | Plant Cell Reports2006，，9： | 1 |
| 19 | Effect of autologous bone marrow stem cells-scaffold transplantation on the ongoing pregnancy rate in intrauterine adhesion women:a randomized,controlled trial显示文摘Intrauterine adhesion is a major cause of female reproductive disorders.Although we and others uncontrolled pilot studies showed that treatment with autologous bone marrow stem cells made a few patients with severe intrauterine adhesion obtain live birth,no large sample randomized controlled studies on this therapeutic strategy in such patients have been reported so far.To verify if the therapy of autologous bone marrow stem cells-scaffold is superior to traditional treatment in moderate to severe intrauterine adhesion patients in increasing their ongoing pregnancy rate,we conducted this randomized controlled clinical trial.Totally 195 participants with moderate to severe intrauterine adhesion were screened and 152 of them were randomly assigned in a 1:1 ratio to either group with autologous bone marrow stem cells-scaffold plus Foley balloon catheter or group with only Foley balloon catheter(control group)from February 2016 to January 2020.The per-protocol analysis included 140 participants:72 in bone marrow stem cells-scaffold group and 68 in control group.The ongoing pregnancy occurred in 45/72(62.5%)participants in the bone marrow stem cells-scaffold group which was significantly higher than that in the control group(28/68,41.2%)(RR=1.52,95%CI 1.08–2.12,P=0.012).The situation was similar in live birth rate(bone marrow stem cells-scaffold group 56.9%(41/72)vs.control group 38.2%(26/68),RR=1.49,95%CI 1.04–2.14,P=0.027).Compared with control group,participants in bone marrow stem cells-scaffold group showed more menstrual blood volume in the 3rd and 6th cycles and maximal endometrial thickness in the 6th cycle after hysteroscopic adhesiolysis.The incidence of mild placenta accrete was increased in bone marrow stem cells-scaffold group and no severe adverse effects were observed.In conclusion,transplantation of bone marrow stem cells-scaffold into uterine cavities of the participants with moderate to severe intrauterine adhesion increased their ongoing pregnancy and live birth rates,and this therapy was relatively safe. | Hui Zhu Taishun Li Peizhen Xu Lijun Ding Xianghong Zhu Bin Wang Xiaoqiu Tang Juan Li Pengfeng Zhu Huiyan Wang Chenyan Dai Haixiang Sun Jianwu Dai Yali Hu | 2024 | Science China(Life Sciences)2024，67，1： | 0 |
| 20 | An improved simple snow-atmosphere-soil transfer model显示文摘On the basis of a simple snow-atmosphere-soil transfer (SAST) model previously developed,this paper presents an improved snow-atmosphere-soil transfer (ISAST) model that has a new numerical scheme and an improved method of layering the snowpack.The new model takes the snow cover temperature and ice content in the snow cover as prognostic variables.This approach,which effectively solves the snow cover temperature distribution when the snow cover is melting or freezing,lessens the iteration time and computation time,which is important for GCM simulation.In this model,the snow cover is divided into three layers (ISAST3) or seven layers (ISAST7).The simulation results obtained using the ISAST7 model agree well with observations in terms of snow depth,snow equivalent water and snow cover lifetime at five Russian sites.The new ISAST model has better simulation capacity for snow cover than the previous SAST model.When the snow cover is deep,the simulation of the ISAST7 model is better than that of the ISAST3 model.Testing shows that our ISAST model is approximately 20% faster than the SAST model. | LIU HuiZhi ZHAI XiaoDong SUN ShuFen FENG JianWu WANG Lei | 2012 | Science China Earth Sciences2012，55，7： | 0 |
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