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| 1 | Antisense MMP-9 RNA inhibits malignant glioma cell growth in vitro and in vivo显示文摘The matrix-degrading metalloproteinases (MMPs), particularly MMP-9, play important roles in the pathogenesis and development of malignant gliomas. In the present study, the oncogenic role of MMP-9 in malignant glioma cells was investigated via antisense RNA blockade in vitro and in vivo. TJ905 malignant glioma cells were transfected with pcDNA3.0 vector expressing antisense MMP-9 RNA (pcDNA-AS-MMP9), which significantly decreased MMP-9 expression, and cell proliferation was assessed. For in vivo studies, U251 cells, a human malignant glioma cell line, were implanted subcutaneously into 4-to 6-week-old BALB/c nude mice. The mice bearing well-established U251 gliomas were treated with intratumoral pcDNA-AS-MMP9-Lipofectamine complex (AS-MMP-9-treated group), subcutaneous injection of endostatin (endostatin-treated group), or both (combined therapy group). Mice treated with pcDNA (empty vector)-Lipofectamine served as the control group. Four or eight weeks later, the volume and weight of tumor, MMP-9 expression, microvessel density and proliferative activity were assayed. We demonstrate that pcDNA-AS-MMP9 significantly decreased MMP-9 expression and inhibited glioma cell proliferation. Volume and weight of tumor, MMP-9 expression, microvessel density and proliferative activity in the antisense-MMP-9-treated and therapeutic alliance groups were significantly lower than those in the control group. The results suggest that MMP-9 not only promotes malignant glioma cell invasiveness, but also affects tumor cell proliferation. Blocking the expression of MMP-9 with antisense RNA substantially suppresses the malignant phenotype of glioma cells, and thus can be used as an effective therapeutic strategy for malignant gliomas. | Cuiyun Sun Qian Wang Hongxu Zhou Shizhu Yu Alain R. Simard Chunsheng Kang Yanyan Li Yanling Kong Tongling An Yanjun Wen Fudong Shi Junwei Hao | 2013 | Neuroscience Bulletin2013，29，1： | 14 |
| 2 | A Classification–Detection Approach of COVID-19 Based on Chest X-ray and CT by Using Keras Pre-Trained Deep Learning Models显示文摘The Coronavirus Disease 2019(COVID-19)is wreaking havoc around the world,bring out that the enormous pressure on national health and medical staff systems.One of the most effective and critical steps in the fight against COVID-19,is to examine the patient’s lungs based on the Chest X-ray and CT generated by radiation imaging.In this paper,five keras-related deep learning models:ResNet50,InceptionResNetV2,Xception,transfer learning and pre-trained VGGNet16 is applied to formulate an classification-detection approaches of COVID-19.Two benchmark methods SVM(Support Vector Machine),CNN(Conventional Neural Networks)are provided to compare with the classification-detection approaches based on the performance indicators,i.e.,precision,recall,F1 scores,confusion matrix,classification accuracy and three types of AUC(Area Under Curve).The highest classification accuracy derived by classification-detection based on 5857 Chest X-rays and 767 Chest CTs are respectively 84%and 75%,which shows that the keras-related deep learning approaches facilitate accurate and effective COVID-19-assisted detection. | Xing Deng Haijian Shao Liang Shi Xia Wang Tongling Xie | 2020 | Computer Modeling in Engineering & Sciences2020，，11： | 4 |
| 3 | Non-equilibrium alloy phase formation and transformation driven by ion beam mixing in the Fe-Hf-Nb multilayers显示文摘In the ion beam mixing experiments,eight Fe-Hf-Nb multilayered films,with overall compositions of Fe67Hf22Nb11,Fe67Hf11Nb22,Fe54Hf38Nb8,Fe54Hf30Nb16,Fe54Hf11Nb35,Fe25Hf67Nb8,Fe25Hf50Nb25 and Fe25Hf11Nb64,were irradiated by 200 keV xenon ions to doses ranging from 3×1014 Xe+/cm2 to 7×1015 Xe+/cm2.The results showed that unique amorphous phases were obtained at designed alloy compositions,falling in the favored glass-forming region deduced from three binary metal sub-systems.Interestingly,at some alloy compositions,the crystal-amorphous-crystal transformations were observed back and forth while varying the irradiation doses.In addition,at the alloy composition of Fe25Hf67Nb8,a metastable FCC phase was formed through an HCP-FCC structural phase transformation and it had a large lattice constant identified to be a=4.51 .Besides,the formation mechanism of non-equilibrium alloy phases was also discussed in terms of thermodynamics of solids and atomic collision theory. | LI Nan WANG TongLe DING Ning DAI Ye BAI Xue LIU BaiXin | 2012 | Science China(Technological Sciences)2012，55，3： | 2 |
| 4 | Comparison of Viromes in Ticks from Different Domestic Animals in China显示文摘Ticks are involved in the transmission of various arboviruses and some tick-borne viruses pose significant threats to the health of humans or livestock.This study aimed to investigate the geographical distribution of tick species and tickassociated viruses in central and eastern China.Total 573 ticks from domestic animals including dogs,sheep and cattle were collected in 2017.Two genera of ticks were identified including Rhipicephalus and Haemaphysalis.Sequencing was performed on Miseq Illumina platform to characterize the tick viromes from the four different sampling locations.Following trimming,13,640 reads were obtained and annotated to 19 virus families.From these sequences,above 37.74%of the viral reads were related to the RNA viruses.Virome comparison study revealed that the tick viral diversity was considerably different in the two identified tick genera.The viral diversity of R.microplus was significantly different from that of other Rhipicephalus species.On the other hand,substantial overlap in viral species was observed between the same genera.In addition,we found no evidence that the natural host played a major role in shaping virus diversity based on the comparison of their viromes.Rather,the geographic location seems to significantly influence the viral families.Phylogenetic study indicated that the novel negative-sense RNA viruses identified in this study was closely related to Bole tick virus 1 and 3 viruses.In conclusion,the present study provides a baseline for comparing viruses detected in ticks,according to species,natural hosts,and geographic locations. | Tingting Zhao Haiyan Gong Xiaojuan Shen Wen Zhang Tongling Shan Xiangqian Yu Seong Jin Wang Li Cui | 2020 | Virologica Sinica2020，35，4： | 2 |
| 5 | CHOICE OF FILLING MATERIALS AND TECHNIQUES IN XINQIAO PYRITE MINE显示文摘CHOICEOFFILLINGMATERIALSANDTECHNIQUESINXINQIAOPYRITEMINE①ZhangQinli,WangXinminColegeofResource,EnvironmentandCivilEnginering,... | Zhang Qinli, Wang Xinmin College of Resource, Environment and Civil Engineering, Central South University of Technology, Changsha 410083 Chen Jiasheng Xinqiao Pyrite Mine, Tongling 244131 | 1997 | 中国有色金属学会会刊:英文版1997，7，4： | 2 |
| 6 | Metallic glass formation in the ternary Ni-Nb-Mo system by ion beam mixing显示文摘Glass forming ability of the ternary Ni-Nb-Mo system was studied by ion beam mixing of the Ni-Nb-Mo multilayered films.In the experiment,metallic glasses i.e.amorphous alloys were formed in the Ni51Nb19Mo30,Ni52Nb35Mo13,Ni61Nb15Mo24 and Ni72Nb20Mo8 multilayered films,while only solid solutions were obtained in the Ni24Nb29Mo47 and Ni26Nb53Mo21 multilayered films.It turned out that the Ni concentration played a dominating role in affecting the glass-forming ability of the system.Besides,thermodynamic calculations predicted a favored composition region for metallic glass formation,matching well with the observations from ion beam mixing. | LI Yang WANG TongLe DING Ning LIU JianBo LIU BaiXin | 2012 | Science China(Technological Sciences)2012，55，8： | 2 |
| 7 | Bex2 regulates cell proliferation and apoptosis in malignant glioma cells via the c-Jun NH2-terminal kinase pathway显示文摘 | Xiuping Zhou Qingming Meng Xuebin Xu Tongle Zhi Qiong Shi Yong Wang Rutong Yu | 2012 | Biochemical and Biophysical Research Communications2012，，3： | 1 |
| 8 | Screening for plant extracts to control potato late blight显示文摘 | WANG Shutong HU Tongle ZHANG Fengqiao | 2007 | Frontiers of Agriculture in China2007，1，1： | 1 |
| 9 | Fusing Carbocycles of Inequivalent Ring Size to a Bis(imino)pyridine-Iron Ethylene Polymerization Catalyst: Distinctive Effects on Activity, PE Molecular Weight, and Dispersity显示文摘The 4,6-bis(arylimino)-1,2,3,7,8,9,10-heptahydrocyclohepta[b]quinoline-iron(II)chlorides(aryl=2,6-Me_(2)C_(6)H_(3) Fe1;2,6-Et_(2)C_(6)H_(3) Fe_(2);2,6-i-Pr_(2)C_(6)H_(3) Fe_(3);2,4,6-Me3C_(6)H_(2) Fe4;and 2,6-Et_(2)-4-Me_(2)C_(6)H_(2) Fe_(5))have been prepared in good yield by a straightforward one-pot reaction of 2,3,7,8,9,10-hexahydro-1H-cyclohepta[b]quinoline-4,6-dione,FeCl_(2)·4H_(2)O,and the appropriate aniline in acetic acid.All ferrous complexes have been characterized by elemental analysis and FT-IR spectroscopy.In addition,the structure of Fe_(3) has been determined by single crystal X-ray diffraction,which showed the iron center to adopt a distorted square pyramidal geometry with the saturated sections of the fused six-and sevenmembered carbocycles to be cis-configured.In combination with either MAO or MMAO,Fe1–Fe5 exhibited exceptionally high activities for ethylene polymerization(up to 15:86×10^(6) g(PE)mol^(−1)(Fe)h^(−1) at 40℃(MMAO)and 9:60×10^(6) g(PE)mol^(−1)(Fe)h^(−1) at 60℃(MAO))and produced highly linear polyethylene(HLPE,Tm≥128℃)with a wide range in molecular weights;in general,the MMAO-promoted polymerizations were more active.Irrespective of the cocatalyst employed,the 2,6-Me_(2)-substituted Fe1 and Fe4 proved the most active while the more sterically hindered 2,6-i-Pr_(2) Fe_(3) the least but afforded the highest molecular weight polyethylene(Mw:65.6–72.6 kg mol^(-1)).Multinuclear NMR spectroscopic analysis of the polymer formed using Fe4/MMAO at 40℃ showed a preference for fully saturated chain ends with a broad bimodal distribution a feature of the GPC trace(Mw/Mn=13:4).By contrast,using Fe4/MAO at 60℃ a vinyl-terminated polymer of lower molecular weight(Mw=14:2 kg mol^(−1))was identified that exhibited a unimodal distribution(Mw/Mn=3:8).Moreover,the amount of aluminoxane cocatalyst employed,temperature,and run time were also found to be influential on the modality of the polymer. | Zheng Wang Gregory A.Solan Yanping Ma Qingbin Liu Tongling Liang Wen-Hua Sun | 2019 | Research2019，，1： | 1 |
| 10 | Synthesis of metallic glasses and metallic glass based composites in the Cu-Mo-Hf system by ion beam mixing显示文摘Single-phase and dual-phase metallic glasses as well as metallic glass based composites were synthesized in the Cu-Mo-Hf ternary metal system by 200 keV xenon ion beam mixing of far-from-equilibrium. It was found that Mo-Hf-based and Cu-Mo-based single-phase metallic glasses could be obtained at compositions around Cu17Mo20Hf63 and Cu34Mo57Hf9, respectively. Interestingly, at the nearly equal-atomic stoichiometry of Cu38Mo31Hf31, a dual-phase Cu-Mo-Hf metallic glass, consisting both of the Mo-Hf-based and Cu-Mo-based phases, was first obtained at relatively low irradiation doses ranging from (1-5)×1015 Xe+/cm2, and a single-phase metallic glass was eventually obtained at a dose of 7×1015 Xe+/cm2. In addition, two glass-based composites were obtained at the compositions of Cu14Mo62Hf24 and Cu77Mo14Hf9, and they consisted of the Mo-Hf based and Cu-Mo based metallic glasses, dissolved with some uniformly distributed BCC Mo-based and FCC Cu-based crystalline solid solutions, respectively. The formation mechanism of the above described non-equilibrium alloy phases was also discussed in terms of the atomic collision theory. | BAI Xue WANG TongLe CUI YuanYuan DING Ning LI JiaHao LIU BaiXin | 2012 | Science China(Technological Sciences)2012，55，5： | 1 |
| 11 | Numerical analysis of consolidation settlement and creep deformation of artificial island revetment structure in a large-scale marine reclamation land project 显示文摘 | ZHAO J ZHOU Tongling WANG Gui-Xuan | 2015 | Polish Maritime Research2015，22，1： | 1 |
| 12 | Virome diversity of ticks feeding on domestic mammals in China显示文摘Ticks are considered the second most common pathogen vectors transmitting a broad range of vital human and veterinary viruses.From 2017 to 2018,640 ticks were collected in eight different provinces in central and western China.Six species were detected,including H.longicornis,De.everestianus,Rh.microplus,Rh.turanicus,Rh.sanguineous,and Hy.asiaticum.Sixty-four viral metagenomic libraries were constructed on the MiSeq Illumina platform,resulting in 13.44 G(5.88×10^(7))of 250-bp-end reads,in which 2,437,941 are viral reads.We found 27 nearly complete genome sequences,including 16 genome sequences encoding entire protein-coding regions(lack of 30 or 50 end non-coding regions)and complete viral genomes,distributed in the arboviral family(Chuviridae,Rhabdoviridae,Nairoviridae,Phenuiviridae,Flaviviridae,Iflaviridae)as well as Parvoviridae and Polyomaviridae that cause disease in mammals and even humans.In addition,13 virus sequences found in Chuviridae,Nairoviridae,Flaviviridae,Iflaviridae,Hepeviridae,Parvoviridae,and Polyomaviridae were identified as belonging to a new virus species in the identified viral genera.Besides,an epidemiological survey shows a high prevalence(9.38%and 15.63%)of two viruses(Ovine Copiparvovirus and Bovine parvovirus 2)in the tick cohort. | Zijun Yang Hao Wang Shixing Yang Xiaochun Wang Quan Shen Likai Ji Jian Zeng Wen Zhang Haiyan Gong Tongling Shan | 2023 | Virologica Sinica2023，38，2： | 0 |
| 13 | High-sensitivity modulation of electromagnetically induced transparency analog in a THz asymmetric metasurface integrating perovskite and graphene显示文摘Active control of the electromagnetically induced transparency(EIT)analog is desirable in photonics development.Here,we theoretically and experimentally proposed a novel terahertz(THz)asymmetric metasurface structure that can possess high-sensitivity modulation under extremely low power density by integrating perovskite or graphene.Using the novel metasurface structure with the perovskite coating,the maximum amplitude modulation depth(AMD)of this perovskite-based device reached 490.53%at a low power density of 12.8037 mW/cm^(2).In addition,after the novel THz metasurface structure was combined with graphene,this graphene-based device also achieved high AMD with the maximum AMD being 180.56%at 16.312 mW/cm^(2),and its transmission amplitude could be electrically driven at a low bias voltage.The physical origin of this modulation was explained using a two-oscillator EIT model.This work provides a promising platform for developing high-sensitivity THz sensors,light modulators,and switches. | TONGLING WANG TENGTENG LI HAIYUN YAO YUYING LU XIN YAN MAOYONG CAO LANJU LIANG MAOSHENG YANG JIANQUAN YAO | 2022 | Photonics Research2022，10，10： | 0 |
| 14 | Path probability for a Brownian motion显示文摘This work reports on numerical simulations of Brownian motion in the non-dissipative limit. The objective was to prove the existence of path probability and to compute probability values for some sample paths. By simulating a large number of particles moving from point to point under Gaussian noise and conservative forces, we numerically determine that the path probability decreases exponentially with increasing Lagrangian action of the paths. | LIN TongLing PUJOS Cyril OU CongJie BI WenPing CALVAYRAC Florent WANG Qiuping Alexandre | 2011 | Chinese Science Bulletin2011，56，34： | 0 |
| 15 | Ultra-sensitive Dirac-point-based biosensing on terahertz metasurfaces comprising patterned graphene and perovskites显示文摘Biosensors are a focus of research on terahertz metasurfaces. However, reports of ultra-sensitive biosensors based on Dirac points are rare. Here, a new terahertz metasurface is proposed that consists of patterned graphene and perovskites. This serves as an ultra-sensitive Dirac-point-based biosensor for qualitative detection of sericin.Theoretically, sericin may make graphene n-doped and drive the Fermi level to shift from the valence band to the Dirac point, causing a dramatic decrease in conductivity. Correspondingly, the dielectric environment on the metasurface undergoes significant change, which is suited for ultra-sensitive biosensing. In addition, metal halide perovskites, which are up-to-date optoelectronic materials, have a positive effect on the phase during terahertz wave transmission. Thus, this sensor was used to successfully detect sericin with a detection limit of 780 pg/m L, achieved by changing the amplitude and phase. The detection limit of this sensor is as much as one order of magnitude lower than that of sensors in published works. These results show that the Dirac-pointbased biosensor is a promising platform for a wide range of ultra-sensitive and qualitative detection in biosensing and biological sciences. | Xin Yan Tengteng Li Guohong Ma Ju Gao Tongling Wang Haiyun Yao Maosheng Yang Lanju Liang Jing Li Jie Li Dequan Wei Meng Wang Yunxia Ye Xiaoxian Song Haiting Zhang Chao Ma Yunpeng Ren Xudong Ren Jianquan Yao | 2022 | Photonics Research2022，10，2： | 0 |
| 16 | Genetic characterization and pathogenicity of a reassortant Eurasian avian-like H1N1 swine influenza virus containing an internal gene cassette from 2009 pandemic H1N1 virus显示文摘Dear editor,Swine influenza virus(SIV)is a member of the Orthomyxoviridae family,influenza A virus genus,which can cause the swine influenza—an acute and highly contagious respiratory disease in pigs(Brown,2000;Kothalawala et al.,2006).The SIV was first observed in 1918 in the United States and the progenitor of the SIV was the H1N1 influenza virus which caused the Spanish influenza pandemic of 1918(Shope,1931). | Shuaiyong Wang Manzhu Wang Lingxue Yu Juan Wang Jiecong Yan Xinli Rong Yanjun Zhou Tongling Shan Wu Tong Guoxin Li Hao Zheng Guangzhi Tong Hai Yu | 2022 | Virologica Sinica2022，37，4： | 0 |
| 17 | Screening of Host Proteins Interacting with PorcineEpidemic Diarrhea Virus (PEDV) N Protein by YeastTwo-hybrid System显示文摘[Objective] The paper was to obtain host proteins interacting with porcine epidemic diarrhea virus(PEDV) N protein. [Method] The recombinant vector pGBKT7-N of PEDV N gene was constructed and used as the bait plasmid to screen the proteins interacting with N protein of PEDV from the cDNA library of porcine alveolar macrophage(PAM) by yeast two-hybrid method. [Result] There was no toxicity and self activation of bait protein in yeast hybridization system, and six proteins(FTH1, LGALS3, CORO1C, SNRPG, KRTAP5-3, ZNF598) interacting with N protein were indentified. It was confirmed that LGALS3 and SNRPG had specific interaction with N protein by return experiment and co-immunoprecipitation(CoIP) test. [Conclusion] The study lays a foundation for further studying the function of PEDV N protein and the pathogenic mechanism of PEDV. | Wang Zhongze Qin Cuili Kong Ning Zuo Yewen Wang Meng Zheng Hao Tong Wu Li Liwei Yu Hai Li Zhili Shan Tongling Tong Guangzhi Li Xue | 2018 | Animal Husbandry and Feed Science2018，10，4： | 0 |
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