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| 1 | BYSL contributes to tumor growth by cooperating with the mTORC2 complex in gliomas显示文摘Objective:BYSL,which encodes the Bystin protein in humans,is upregulated in reactive astrocytes following brain damage and/or inflammation.We aimed to determine the role and mechanism of BYSL in glioma cell growth and survival.Methods:BYSL expression in glioma tissues was measured by quantitative real-time PCR,Western blot,and immunohistochemistry.In vitro assays were performed to assess the role of BYSL in cell proliferation and apoptosis.Protein interactions and co-localization were determined by co-immunoprecipitation and double immunofluorescence.The expression and activity of the AKT/m TOR signaling molecules were determined by Western blot analysis,and the role of BYSL in glioma growth was confirmed in an orthotopic xenograft model.Results:The BYSL m RNA and protein levels were elevated in glioma tissues.Silencing BYSL inhibited glioma cell proliferation,impeded cell cycle progression,and induced apoptosis,whereas overexpressing BYSL protein led to the opposite effects.We identified a complex consisting of BYSL,RIOK2,and m TOR,and observed co-localization and positive correlations between BYSL and RIOK2 in glioma cells and tissues.Overexpressing BYSL or RIOK2 increased the expression and activity of AKT/m TOR signaling molecules,whereas downregulation of BYSL or RIOK2 decreased the activity of AKT/m TOR signaling molecules.Silencing BYSL or RIOK2 decreased the growth of the tumors and prolonged the lifespan of the animals in an orthotopic xenograft model.Conclusions:High expression of BYSL in gliomas promoted tumor cell growth and survival both in vitro and in vivo.These effects could be attributed to the association of BYSL with RIOK2 and m TOR,and the subsequent activation of AKT signaling. | Shangfeng Gao Zhuang Sha Junbo Zhou Yihao Wu Yunnong Song Cheng Li Xuejiao Liu Tong Zhang Rutong Yu | 2021 | Cancer Biology & Medicine2021，18，1： | 3 |
| 2 | Anisotropic Total Variation Regularization Based NAS-RIF Blind Restoration Method for OCT Image显示文摘Based on anisotropic total variation regularization(ATVR), a nonnegativity and support constraints recursive inverse filtering(NAS-RIF) blind restoration method is proposed to enhance the quality of optical coherence tomography(OCT) image. First, ATVR is introduced into the cost function of NAS-RIF to improve the noise robustness and retain the details in the image.Since the split Bregman iterative is used to optimize the ATVR based cost function, the ATVR based NAS-RIF blind restoration method is then constructed. Furthermore, combined with the geometric nonlinear diffusion filter and the Poisson-distribution-based minimum error thresholding, the ATVR based NAS-RIF blind restoration method is used to realize the blind OCT image restoration. The experimental results demonstrate that the ATVR based NAS-RIF blind restoration method can successfully retain the details in the OCT images. In addition, the signal-to-noise ratio of the blind restored OCT images can be improved, along with the noise robustness. | Xuesong Fu Jianlin Wang Zhixiong Hu Yongqi Guo Kepeng Qiu Rutong Wang | 2020 | Journal of Beijing Institute of Technology2020，29，2： | 2 |
| 3 | Bex2 regulates cell proliferation and apoptosis in malignant glioma cells via the c-Jun NH2-terminal kinase pathway显示文摘 | Xiuping Zhou Qingming Meng Xuebin Xu Tongle Zhi Qiong Shi Yong Wang Rutong Yu | 2012 | Biochemical and Biophysical Research Communications2012，，3： | 1 |
| 4 | Deciphering the pharmacological mechanism of Guan-Jie-Kang in treating rat adjuvant-induced arthritis using omics analysis显示文摘Traditional Chinese medicine (TCM) formulas have attracted increasing attention worldwide in the past few years for treating complex disease including rheumatoid arthritis. However, their mechanisms are complex and remain unclear. Guan-Jie-Kang (GJK), a prescription modified from “Wu Tou Decoction,” was found to significantly relieve arthritis symptoms in rats with adjuvant-induced arthritis after 30-day treatment, especially in the 24 g/kg/day group. By analyzing 1749 targets related to 358 compounds in the five herbs of GJK, we identified the possible anti-arthritis pathways of GJK, including the calcium signaling and metabolic pathways. Bone damage levels were assessed by micro-computed tomography, and greater bone protective effect was observed with GJK treatment than with methotrexate. Receptor activator of nuclear factor kB ligand (RANKL)-RANK signaling, which is related to calcium signaling, was significantly regulated by GJK. Moreover, a target metabolomics assay of serum was conducted;17 metabolic biomarkers showed significant correlations with treatment. An integrated pathway analysis revealed that pyruvate metabolism, purine metabolism, and glycolysis metabolism were significantly associated with the effects of GJK in arthritis treatment. Thus, this study establishes a new omics analytical method integrated with bioinformatics analysis for elucidating the multi-pathway mechanisms of TCM. | Hudan Pan Yanfang Zheng Zhongqiu Liu Zhongwen Yuan Rutong Ren Hua Zhou Ying Xie Liang Liu | 2019 | Frontiers of Medicine2019，13，5： | 1 |
| 5 | Isolation,cultivation and identification of brain glioma stem cells by magnetic bead sorting显示文摘This study describes a detailed process for obtaining brain glioma stem cells from freshly dissected human brain glioma samples using an immunomagnetic bead technique combined with serum-free media pressure screening.Furthermore,the proliferation,differentiation and self-renewal biological features of brain glioma stem cells were identified.Results showed that a small number of CD133 positive tumor cells isolated from brain glioma samples survived as a cell suspension in serum-free media and proliferated.Subcultured CD133 positive cells maintained a potent self-renewal and proliferative ability,and expressed the stem cell-specific markers CD133 and nestin.After incubation with fetal bovine serum,the number of glial fibrillary acidic protein and microtubule associated protein 2 positive cells increased significantly,indicating that the cultured brain glioma stem cells can differentiate into astrocytes and neurons.Western blot analysis showed that tumor suppressor phosphatase and tensin homolog was highly expressed in tumor spheres compared with the differentiated tumor cells.These experimental findings indicate that the immunomagnetic beads technique is a useful method to obtain brain glioma stem cells from human brain tumors. | Xiuping Zhou Chao Zheng Qiong Shi Xiang Li Zhigang Shen Rutong Yu | 2012 | Neural Regeneration Research2012，7，13： | 1 |
| 6 | Transcriptome analysis of Clematis lanuginosa: Novel features of the molecular events occurring under heat-shock stress显示文摘The weak heat tolerance of Clematis ornamental varieties negatively affects their ornamental qualities in the summer.To elucidate heat resistance mechanisms,Clematis lanuginosa,which is an important original parent of the Clematis large-flowered group of ornamental varieties,was selected for use in this study.Here,six libraries,including three biological replicates each of control and heat-shock stress samples,were determined using RNA-sequencing technology.In total,62,050 unigenes were obtained,and 6,439 unigenes exceeded 1 kb in length.A total of 42,377 unigenes were annotated using six databases.Between the two treatments,2,165 differentially expressed genes were identified,with 1,565 being up-regulated and 600 down-regulated.In addition,51 heat-shock protein-encoding genes were identified,among which the small heat-shock proteins accounted for 68.63%.In total,two heat-shock factors and 12 ribosomal proteins were significantly up-regulated under heatstress conditions.The differential expressions of ethylene-responsive transcription factor,chalcone synthase,cysteine-rich receptor-like kinase and aspartic protease unigenes in guard cells were induced by heat-shock.The data obtained will assist the elucidation of the molecular events underlying heat-stress responses in C.lanuginosa. | Linfang Li Shuan Wang Qiao Li Linghe Sun Xin Han Lulu Gao Peng Wang Fenni Lv Rutong Yang Ya Li | 2022 | Ornamental Plant Research2022，2，1： | 0 |
| 7 | Polaron-assisted nonadiabatic dynamics in protonated TiO_(2) with surface water molecule显示文摘We investigated the polaron-assisted nonadiabatic dynamics in protonated TiO_(2),as well as the polaron-H_(2)O coupling and its effects on the relaxation of photogenerated electrons.We observed that different polaron hopping regimes result in varied nonadiabatic couplings and relaxations of excited electrons from the conduction band minimum to the gap states of protonated TiO_(2),with a weak dependence on the actual trapping site of the polaron.Surface-adsorbed H_(2)O molecules can attract polarons toward the adsorbed Ti sites,with the coupling between H_(2)O and the polaron being inversely proportional to their distance.Our findings suggest that the lifetime of the photogenerated charge carriers can be extended by reducing the polaron-H_(2)O distances,with expected benefits to the efficiency of the reduced TiO 2 samples for photocatalytic applications. | Zhongfei Xu Chuanjia Tong Rutong Si Gilberto Teobaldi Limin Liu | 2023 | ChemPhysMater2023，2，4： | 0 |
| 8 | CACA guidelines for holistic integrative management of glioma显示文摘Glioma of the brain is a kind of tumor originating from neuroglial cells.It is the most common primary intracranial tumor,accounting for~30%of all central nervous system tumors and 80% of malignant brain tumors.Glioma is characterized by high disability and recurrence rates.The disease seriously threatens the life of patients,afects their quality of life,and brings a heavy economic and psychological burden to patients,families,and society.With the progression of molecular genetic testing technology and the completion of various clinical trials,the classifcation scheme for glioma is increasingly well established.Diagnosis and treatment regimens,including traditional and new regimens,are becoming increasingly specialized and standardized.The purpose is to develop a clinical diagnosis and treatment guideline for glioma in the Chinese population suitable for Chinese doctors and the general population based on domestic and international glioma research progress.Thus,domestic practitioners in the feld can obtain current information and provide better service to patients with glioma,promoting the development of domestic clinical medicine and basic research on glioma. | Daiming Fan Tao Jiang Wenbin Ma Chuanlu Jiang Yongping You Ying Mao Xiaoguang Qiu Chunsheng Kang Gang Li Qing Mao Xuejun Yang Zhiyong Qin Zhixiong Liu Weimin Wang Xinting Wei Wenbin Li Yunhui Liu Rutong Yu Xinguang Yu Dezhi Kang Yonggao Mou Lei Wang Wei Zhang Zhaoshi Bao Ruichao Chai Baoshi Chen Xing Fan Shengyu Fang Guanzhang Li Lianwang Li Shouwei Li Xing Liu Yanwei Liu Xia Shan Liang Wang Yinyan Wang Yu Wang Zheng Wang Zhiliang Wang Chenxing Wu Wei Yan Pei Yang Gan You Chuanbao Zhang Zhong Zhang Zheng Zhao | 2022 | Holistic Integrative Oncology2022，1，1： | 0 |
| 9 | A multicenter, prospective, randomized clinical study to evaluate the efficacy and safety of fibrin sealant as an adjunct to sutured dural repair显示文摘To the Editor:Cerebrospinal fluid(CSF)leakage increases the risk of post-operative complications following cranial surgery,and its progression is not always predictable.Currently,surgeons use various methods to achieve watertight closure of CSF,including synthetic sealants,collagen or gelatin based sponges,[1]and fibrin sealants(FS).[2,3]Bioseal(Porcine Fibrin Sealant Kit,Guangzhou Bioseal Biotech Co.,Ltd.,Guangzhou,China)is a virus-inactivated porcine plasma-derived FS that has been approved as a supportive treatment to control surgical bleeding. | Rutong Yu Wei Zhu Richard Kocharian Bogdan Ilie Zhong Wang Dezhi Kang Gang Zhao Hua Yang Kai Shu Xianzhi Liu Yiquan Ke Shun Qu Ying Mao | 2022 | Chinese Medical Journal2022，135，20： | 0 |
| 10 | Diversity Assessment of an Endemic Carpinus oblongifolia(Betulaceae)Using Specific-Locus Amplified Fragment Sequencing and Implications for Conservation显示文摘Carpinus oblongifolia is an endemic species and the extant wild populations show a fragmentation distribution in the Baohua Mountain of Jiangsu Province in eastern China.Understanding of genetic diversity plays an important role in C.oblongifolia survival and sustainable development.The wild C.oblongifolia population was artificially divided into four subpopulations according to the microhabitats,and another two subpopulations were constructed by progeny seedlings cultivated with the mature seeds.Then,the leaf buds of 80 individuals from six subpopulations were sampled to develop single nucleotide polymorphisms(SNPs)using specific-locus amplified fragment sequencing(SLAF-seq).Based on these SNPs,we aimed to characterize the genetic diversity and population structure of C.oblongifolia and provide an illumination and reference for effective management of such a small endemic population.The level of genetic diversity was low at the species level,and the progeny subpopulations had a relatively higher genetic diversity than the wild subpopulations.This may be attributed to a high gene flow and an excess heterozygosity to reduce the threat of genetic drift-based hazards.Moreover,the progeny subpopulations had the ability to form new clusters and a great contribution to the genetic structure variation of C.oblongifolia.These results will assist with the development of conservation and management strategies,such as properly evacuating competitive trees to provide more chance for pollen and seed flow in situ conservation,and establishing sufficient seedling plantlets under laboratory conditions for reintroduction to enlarge the effective population size. | Sumei Li Qing Wang Peng Wang Shuan Wang Rutong Yang Ya Li | 2022 | Phyton-International Journal of Experimental Botany2022，91，3： | 0 |
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