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| 1 | The Hardy Rubber Tree Genome Provides Insights into the Evolution of Polyisoprene Biosynthesis显示文摘Eucommia ulmoides,也叫的强壮的橡胶树,是一棵经济地重要的树;然而,它的染色体顺序的缺乏 ?限制基本生物研究和这植物种的应用研究。这里,我们在场它的 1.2-Gb 染色体的一个高质量的集会(支架 N50 ?=? 1.88 Mb ) 与至少 26 ? 为 E 的 723 预言的基因。ulmoides,顺序 Garryales 的首先定序的染色体,它用联合定序的 Illumina 的综合策略被获得,定序的 PacBio,和印射的 BioNano。作为到 lamiids 和 campanulids 的姐妹 taxon, E。ulmoides 经历了三倍由核心 eudicots 而是没有进一步整个染色体的复制分享了的一个古老的染色体 ? 在最后 1.25 亿年里。E。ulmoides 为涉及压力回答和第二等的代谢物的生合成的多重基因展出高表达式层次或基因数字扩大,它可以说明它的可观的环境适应性。与橡胶树(Hevea brasiliensis ) 相对照,它生产 cis 聚异式戊二我烯, E。ulmoides 演变综合经由 farnesyl diphosphate synthases (FPS ) 的长链的 trans 聚异式戊二我烯。而且, FPS 和橡胶延伸 factor/small 橡胶粒子蛋白质基因家庭从 H 独立地被扩展。brasiliensis 系。这些结果提供新卓见进 E. 的生物学 ? ulmoides 和聚异式戊二我烯生合成的起源。 | Ta-na Wuyun Lin Wang Huimin Liu Xuewen Wang Liangsheng Zhang Jeffrey L. Bennetzen Tiezhu Li Lirong Yang Panfeng Liu Lanying Du Lu Wang Mengzhen Huang Jun Qing Iili Zhu Wenquan Bao Hongguo Li Qingxin Du Jingle Zhu Hong Yang Shuguang Yang Hui Liu Hui Yue Jiang Hu Suoliang Yu Yu Tian Fan Liang Jingjing Hu Depeng Wang Ruiwen Gao Dejun Li Hongyan Du | 2018 | Molecular Plant2018，11，3： | 24 |
| 2 | Synthesis of hydrophobic carbon nanotubes/reduced graphene oxide composite films by flash light irradiation显示文摘 | Kai Wang Jinbo Pang Liwei Li Shengzhe Zhou Yuhao Li Tiezhu Zhang | 2018 | Frontiers of Chemical Science and Engineering2018，12，3： | 10 |
| 3 | SNX11 Identified as an Essential Host Factor for SFTS Virus Infection by CRISPR Knockout Screening显示文摘Severe fever with thrombocytopenia syndrome virus(SFTSV)is a highly pathogenic tick-borne bunyavirus that causes lethal infectious disease and severe fever with thrombocytopenia syndrome(SFTS)in humans.The molecular mechanisms and host cellular factors required for SFTSV infection remain uncharacterized.Using a genome-wide CRISPR-based screening strategy,we identified a host cellular protein,sorting nexin 11(SNX11)which is involved in the intracellular endosomal trafficking pathway,as an essential cell factor for SFTSV infection.An SNX11-KO HeLa cell line was established,and SFTSV replication was significantly reduced.The glycoproteins of SFTSV were detected and remained in later endosomal compartments but were not detectable in the endoplasmic reticulum(ER)or Golgi apparatus.pH values in the endosomal compartments of the SNX11-KO cells increased compared with the pH of normal HeLa cells,and lysosomal-associated membrane protein 1(LAMP1)expression was significantly elevated in the SNX11-KO cells.Overall,these results indicated that penetration of SFTSV from the endolysosomes into the cytoplasm of host cells was blocked in the cells lacking SNX11.Our study for the first time provides insight into the important role of the SNX11 as an essential host factor in the intracellular trafficking and penetrating process of SFTSV infection via potential regulation of viral protein sorting,membrane fusion,and other endocytic machinery. | Tiezhu Liu Jiajia Li Yang Liu Yuanyuan Qu Aqian Li Chuan Li Quanfu Zhang Wei Wu Jiandong Li Yan Liu Dexin Li Shiwen Wang Mifang Liang | 2019 | Virologica Sinica2019，34，5： | 3 |
| 4 | Rapid Urbanization Induced Extensive Forest Loss to Urban Land in the Guangdong-Hong Kong-Macao Greater Bay Area, China显示文摘China has experienced rapid urbanizations with dramatic land cover changes since 1978. Forest loss is one of land cover changes, and it induces various eco-environmental degradation issues. As one of China’s hotspot regions, the Guangdong-Hong KongMacao Greater Bay Area(GBA) has undergone a dramatic urban expansion. To better understand forest dynamics and protect forest ecosystem, revealing the processes, patterns and underlying drivers of forest loss is essential. This study focused on the spatiotemporal evolution and potential driving factors of forest loss in the GBA at regional and city level. The Landsat time-series images from 1987 to2017 were used to derive forest, and landscape metrics and geographic information system(GIS) were applied to implement further spatial analysis. The results showed that: 1) 14.86% of the total urban growth area of the GBA was obtained from the forest loss in1987–2017;meanwhile, the forest loss area of the GBA reached 4040.6 km2, of which 25.60%(1034.42 km2) was converted to urban land;2) the percentages of forest loss to urban land in Dongguan(19.14%), Guangzhou(18.35%) and Shenzhen(15.81%) were higher than those in other cities;3) the forest became increasingly fragmented from 1987–2007, and then the fragmentation decreased from2007 to 2017);4) the landscape responses to forest changes varied with the scale;and 5) some forest loss to urban regions moved from low-elevation and gentle-slope terrains to higher-elevation and steep-slope terrains over time, especially in Shenzhen and Hong Kong.Urbanization and industrialization greatly drove forest loss and fragmentation, and, notably, hillside urban land expansion may have contributed to hillside forest loss. The findings will help policy makers in maintaining the stability of forest ecosystems, and provide some new insights into forest management and conservation. | YANG Chao LIU Huizeng LI Qingquan CUI Aihong XIA Rongling SHI Tiezhu ZHANG Jie GAO Wenxiu ZHOU Xiang WU Guofeng | 2021 | Chinese Geographical Science2021，31，1： | 3 |
| 5 | Amplicon-Based Illumina Sequencing and Quantitative PCR Reveals Nanoplankton Diversity and Biomass in Surface Water of Qinhuangdao Coastal Area, China显示文摘Aureococcus anophagefferens caused brown tides for three consecutive years from 2009 to 2011 in the coastal waters of Qinhuangdao, China, with numerous, widespread ecological and economic impact on ecosystems. To understand the population dy- namics of nanoplankton during the brown tides, sequences of the V9 region of the 18S rDNA gene, used as a marker, were analyzed by Illumina sequencing to assess nanoplankton biomass, and real-time fluorescence quantitative PCR was performed to analyze spa- tial variation in the 18S rDNA copy concentrations of nanoplankton off the Qinhuangdao coast in July, 2011. The results showed that A. anophagefferens and Minutocellus polymorphus were the dominant species in the local phytoplankton community during the brown tide in July 2011. The highest 18S rDNA copy concentrations of A. anophagefferens and M. polymorphus were detected at stations SHG and FN, respectively. The central area most strongly affected by the brown tide migrated southward from 2011 to 2013. Redundancy analysis (RDA) showed that the decreasing NOx concentration might provide suitable nutrient conditions for the A. anophagefferens outbreak. During the brown tide caused by A. anophagefferens, other phytoplankton, such as diatoms, cryptophytes, chlorophytes, dinoflagellates and other flagellates, could co-occur with it. For zooplankton, due to less selective feeding behavior, Amoebozoa was the most abundant zooplankton at station SHG, while Ciliophora was the most abundant zooplankton at other sta- tions for its more selective feeding. | QIAO Ling YU Jie LI Ying GUO Haobing ZHEN Yu ZHANG Lingling MI Tiezhu BAO Zhenmin XU Xiaochun | 2019 | Journal of Ocean University of China2019，18，4： | 2 |
| 6 | Microbial Community Composition and Function in Sediments from the Pearl River Mouth Basin显示文摘This study was conducted to characterize the diversity and function of microbial communities in marine sediments of the Pearl River Mouth Basin(PRMB)in the South China Sea.The results showed that the bacterial and archaeal communities varied greatly with depth.Proteobacteria in bacterial communities and Nitrososphaeria and Woesearchaeota in archaeal communities were dominant in the shallow sediments(1-40 cm),while Chloroflexi in bacterial communities and Bathyarchaeia in archaeal communities were dominant in the deep sediments(50-200 cm).Regarding ecological functions based on the metatranscriptomic data,genes involved in various pathways of nitrogen metabolism and sulfur metabolism were observed in the tested sediment samples.Metagenomic analysis revealed that Proteobacteria contribute the most to nearly all genes involved in nitrogen and sulfur metabolism.Moreover,Thaumarchaeota contribute the most to certain genes involved in nitrification,denitrification and assimilatory sulfate reduction pathways.The most abundant bacterial genus,Candidatus Scalindua,is crucial for nitrification,dissimilatory nitrate reduction,denitrification and assimilatory sulfate reduction pathways. | CHEN Ye MI Tiezhu LIU Yueteng LI Siqi ZHEN Yu | 2020 | Journal of Ocean University of China2020，19，4： | 2 |
| 7 | Wearable and flexible electrochemical sensors for sweat analysis:a review显示文摘Flexible wearable sweat sensors allow continuous,real-time,noninvasive detection of sweat analytes,provide insight into human physiology at the molecular level,and have received significant attention for their promising applications in personalized health monitoring.Electrochemical sensors are the best choice for wearable sweat sensors due to their high performance,low cost,miniaturization,and wide applicability.Recent developments in soft microfluidics,multiplexed biosensing,energy harvesting devices,and materials have advanced the compatibility of wearable electrochemical sweat-sensing platforms.In this review,we summarize the potential of sweat for medical detection and methods for sweat stimulation and collection.This paper provides an overview of the components of wearable sweat sensors and recent developments in materials and power supply technologies and highlights some typical sensing platforms for different types of analytes.Finally,the paper ends with a discussion of the challenges and a view of the prospective development of this exciting field. | Fupeng Gao Chunxiu Liu Lichao Zhang Tiezhu Liu Zheng Wang Zixuan Song Haoyuan Cai Zhen Fang Jiamin Chen Junbo Wang Mengdi Han Jun Wang Kai Lin Ruoyong Wang Mingxiao Li Qian Mei Xibo Ma Shuli Liang Guangyang Gou Ning Xue | 2023 | Microsystems & Nanoengineering2023，9，1： | 2 |
| 8 | Characterization of microbial communities in sediments of the South Yellow Sea显示文摘Illumina sequencing and quantitative PCR(qPCR)based on the 16 S ribosomal RNA(rRNA)gene were conducted to characterize the vertical distribution of bacterial and archaeal communities in the sediments of two sites from the South Yellow Sea.Both bacterial and archaeal communities showed a clear stratified distribution with sediment depth.The microbial communities in the upper layers were distinct from those in the deeper layers;the relative abundances of sequences of Thaumarchaeota,Gammaproteobacteria,and Actinobacteria were higher in the upper than in the deeper sediments,whereas the sequences of Bathyarchaeia,Lokiarchaeota,Euryarchaeota,Chloroflexi,and Deltaproteobacteria were relatively more abundant in the deeper sediments.Sediment depth and total organic carbon(TOC)can significantly influence both the bacterial and archaeal communities.Furthermore,bacterial and archaeal groups potentially involved in nitrogen,sulfur,and methane metabolism were detected in both sites.In our study,both ammonia-oxidizing bacteria(Nitrospira)and ammonia-oxidizing archaea(Candidatus Nitrosopumilus)were responsible for ammonia oxidization.Additionally,sulfur-reducing bacteria SEEP-SRB1 forming consortia with anaerobic methane-oxidizing archaea ANME-2 a-2 b were capable of anaerobic methane oxidation(AOM)in the 3400-02 sediment samples. | Ye CHEN Siqi LI Xiaoqing XU Manman MA Tiezhu MI Yu ZHEN Zhigang YU | 2021 | Journal of Oceanology and Limnology2021，39，3： | 2 |
| 9 | Manufacture of Cheddar cheese using probiotic Lactobacillus plantarum K25 and its cholesterol-lowering effects in a mice model显示文摘 | Li Zhang Xue Zhang Chunhong Liu Changying Li Shengyu Li Tiezhu Li Da Li Yujuan Zhao Zhennai Yang | 2013 | World Journal of Microbiology and Biotechnology2013，，1： | 1 |
| 10 | Manufacture of Cheddar cheese using probiotic Lactobacillus plantarum K25 and its cholesterol-lowering effects in a mice model显示文摘 | Li Zhang Xue Zhang Chunhong Liu Changying Li Shengyu Li Tiezhu Li Da Li Yujuan Zhao Zhennai Yang | 2013 | World Journal of Microbiology and Biotechnology2013，，1： | 1 |
| 11 | Electrochemical Proton Storage:From Fundamental Understanding to Materials to Devices显示文摘Simultaneously improving the energy density and power density of electrochemical energy storage systems is the ultimate goal of electrochemical energy storage technology.An effective strategy to achieve this goal is to take advantage of the high capacity and rapid kinetics of electrochemical proton storage to break through the power limit of batteries and the energy limit of capacitors.This article aims to review the research progress on the physicochemical properties,electrochemical performance,and reaction mechanisms of electrode materials for electrochemical proton storage.According to the different charge storage mechanisms,the surface redox,intercalation,and conversion materials are classified and introduced in detail,where the influence of crystal water and other nanostructures on the migration kinetics of protons is clarified.Several reported advanced full cell devices are summarized to promote the commercialization of electrochemical proton storage.Finally,this review provides a framework for research directions of charge storage mechanism,basic principles of material structure design,construction strategies of full cell device,and goals of practical application for electrochemical proton storage. | Tiezhu Xu Di Wang Zhiwei Li Ziyang Chen Jinhui Zhang Tingsong Hu Xiaogang Zhang Laifa Shen | 2022 | Nano-Micro Letters2022，14，8： | 1 |
| 12 | Comparison of fine particles emissions of light duty gasoline vehicles from chassis dynamometer tests and on-road measurements显示文摘 | LI Tiezhu CHEN Xudong YAN Zhenxing | 2013 | At- mospherie Environment2013，68，2： | 1 |
| 13 | Composite membranes based on highly sulfonated PEEK and PBI:Morphology characteristics and performance显示文摘 | Haiqiu Zhang Xianfeng Li Chengji Zhao Tiezhu Fu Yuhua Shi Hui Na | 2008 | Journal of Membrane Science2008，308，： | 1 |
| 14 | Four novel frameworks built by imidazole-based dicarboxylate ligands: Hydro(solvo)thermal synthesis, crystal structures, and properties显示文摘 | ZHANG FUWEI LI ZIFENG GE TIEZHU | 2010 | Inorg Chem2010，49，： | 1 |
| 15 | Robust Topology Optimization of Periodic Multi-Material Functionally Graded Structures under Loading Uncertainties显示文摘This paper presents a robust topology optimization design approach for multi-material functional graded structures under periodic constraint with load uncertainties.To characterize the random-field uncertainties with a reduced set of random variables,the Karhunen-Lo`eve(K-L)expansion is adopted.The sparse grid numerical integration method is employed to transform the robust topology optimization into a weighted summation of series of deterministic topology optimization.Under dividing the design domain,the volume fraction of each preset gradient layer is extracted.Based on the ordered solid isotropic microstructure with penalization(Ordered-SIMP),a functionally graded multi-material interpolation model is formulated by individually optimizing each preset gradient layer.The periodic constraint setting of the gradient layer is achieved by redistributing the average element compliance in sub-regions.Then,the method of moving asymptotes(MMA)is introduced to iteratively update the design variables.Several numerical examples are presented to verify the validity and applicability of the proposed method.The results demonstrate that the periodic functionally graded multi-material topology can be obtained under different numbers of sub-regions,and robust design structures are more stable than that indicated by the deterministic results. | Xinqing Li Qinghai Zhao Hongxin Zhang Tiezhu Zhang Jianliang Chen | 2021 | Computer Modeling in Engineering & Sciences2021，，5： | 1 |
| 16 | Manufacture of Cheddar cheese using probiotic Lactobacillus plantarum K25 and its cholesterol-lowering effects in a mice model显示文摘 | Li Zhang Xue Zhang Chunhong Liu Changying Li Shengyu Li Tiezhu Li Da Li Yujuan Zhao Zhennai Yang | 2013 | World Journal of Microbiology and Biotechnology2013，，1： | 1 |
| 17 | Degradation potential and diversity of oil-degrading bacteria isolated from the sediments of the Jiaozhou Bay, China显示文摘A great deal of oil contaminated the shoreline by the Qingdao oil pipeline explosion in 2013. The four oildegrading consortia were enriched from sediment samples with crude oil as sole carbon and energy sources. The biodiversity and community analysis showed that the Luteibacter, Parvibaculum and a genus belonging to Alcanivoracaceae were found predominant bacteria in the four consortia, which belonged to Proteobacteria. Nine strains exhibiting distinct 16S rRNA gene sequences were isolated from the consortia. These strains were identified to eight genera based on 16S rRNA gene sequences. Five of the nine strains degraded more than 30% of the crude oil in two weeks by gravimetric method. From the analysis of GC-MS, most of the isolated strains tended to degrade n-alkanes rather than PAHs. Five strains showed high degrading ability of the total n-alkanes. Only Strain D2 showed great PAHs degrading ability and the degrading rates ranged from 34.9% to 77.5%. The sequencing analysis of the oil-degrading consortia confirmed that the genus of Alcanivorax was one of the dominant bacteria in Consortia A and E and Strain E4 might be one of the dominant bacteria. The strains obtained in this study demonstrated the potential for oil bioremediation in oil-contaminated beach ecosystems. | Wei Gao Xiangxing Gao Tiezhu Mi Bin Han Yiran Zhang Xinzi Li Xiaofei Yin Chengjun Sun Qian Li Zhisong Cui Xiao Luan Zhigang Yu Li Zheng | 2019 | Acta Oceanologica Sinica2019，38，6： | 1 |
| 18 | Transformation and source of nutrients in the Changjiang Estuary显示文摘The concentrations of phosphate(PO43-),ammonium,nitrite,nitrate,silicate,dissolved organic nitrogen(DON),dissolved organic phosphorus(DOP),particulate phosphorus(PP)and particulate nitrogen(PN)along the salinity gradient were measured in the Changjiang Estuary in April 2007.The behavior of nutrient species along the continuum from the freshwater to the coastal zone is discussed.In the mixing zone between the riverine and marine waters,nitrate and phosphate behave non-conservatively,while silicate behaves conservatively.Nutrient import was quantified from the river load.Nutrient export to the sea was quantified from river discharge and from the salinity-nutrient gradient in the outer estuary.Using these data,a nitrogen and phosphorus budget was made.The internal estuarine fluxes played an important role in the nutrient estuarine fluxes,which accounted for approximately 41%of the nitrogen flux and 45%of the phosphorus flux.The mixing experiments in the laboratory generally reproduced well the inorganic process affecting nutrient dynamics in the Changjiang Estuary,indicating that the primary P and N transformation processes were phosphate and nitrate desorption along the salinity gradient. | YAO QingZhen YU ZhiGang LI LingLing CHEN HongTao MI TieZhu | 2014 | Science China Chemistry2014，57，5： | 1 |
| 19 | Traffic condition for bus lanesetting on urban arteries 显示文摘 | LI Tiezhu DING Jianyou SUN Yunfeng | 2010 | Journal of Kunming University of Science and Technology: Science and Technology2010，35，1： | 1 |
| 20 | 显示文摘 | Ding Tiezhu Li Jian Qi Qige | 2003 | Journal of Rare Earths2003，21，4： | 1 |
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