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| 1 | Human endogenous retrovirus W env increases nitric oxide production and enhances the migration ability of microglia by regulating the expression of inducible nitric oxide synthase显示文摘Human endogenous retrovirus W env(HERV-W env) plays a critical role in many neuropsychological diseases such as schizophrenia and multiple sclerosis(MS). These diseases are accompanied by immunological reactions in the central nervous system(CNS). Microglia are important immunocytes in brain inflammation that can produce a gasotransmitter – nitric oxide(NO). NO not only plays a role in the function of neuronal cells but also participates in the pathogenesis of various neuropsychological diseases. In this study, we reported increased NO production in CHME-5 microglia cells after they were transfected with HERV-W env. Moreover, HERV-W env increased the expression and function of human inducible nitric oxide synthase(hi NOS) and enhanced the promoter activity of hi NOS. Microglial migration was also enhanced. These data revealed that HERV-W env might contribute to increase NO production and microglial migration ability in neuropsychological disorders by regulating the expression of inducible NOS. Results from this study might lead to the identification of novel targets for the treatment of neuropsychological diseases, including neuroinflammatory diseases, stroke, and neurodegenerative diseases. | Ran Xiao Shan Li Qian Cao Xiuling Wang Qiujin Yan Xiaoning Tu Ying Zhu Fan Zhu | 2017 | Virologica Sinica2017，32，3： | 3 |
| 2 | Human leukemia antigen-A0201-restricted epitopes of humanendogenous retrovirus W family envelope(HERV-W env)inducestrong cytotoxic T lymphocyte responses显示文摘Human endogenous retrovirus W family(HERV-W) envelope(env) has been reported to be related to several human diseases, including autoimmune disorders, and it could activate innate immunity.However, there are no reports investigating whether human leukemia antigen(HLA)-A~*0201^+restriction is involved in the immune response caused by HERV-W env in neuropsychiatric diseases. In the present study, HERV-W env-derived epitopes presented by HLA-A~*0201 are described with the potential for use in adoptive immunotherapy. Five peptides displaying HLAA~*0201-binding motifs were predicted using SYFEPITHI and BIMAS, and synthesized. A CCK-8 assay showed peptides W, Q and T promoted lymphocyte proliferation. Stimulation of peripheral blood mononuclear cells from HLA-A~*0201^+ donors with each of these peptides induced peptidespecific CD8^+ T cells. High numbers of IFN-γ-secreting T cells were also detectable after several weekly stimulations with W, Q and T. Besides lysis of HERV-W env-loaded target cells, specific apoptosis was also observed. These data demonstrate that human T cells can be sensitized toward HERV-W env peptides(W, Q and T) and, moreover, pose a high killing potential toward HERV-W env-expressing U251 cells. In conclusion, peptides W Q and T, which are HERV-W env antigenic epitopes, have both antigenicity and immunogenicity, and can cause strong T cell immune responses. Our data strengthen the view that HERV-W env should be considered as an autoantigen that can induce autoimmunity in neuropsychiatric diseases, such as multiple sclerosis and schizophrenia. These data might provide an experimental foundation for a HERV-W env peptide vaccine and new insight into the treatment of neuropsychiatric diseases. | Xiaoning Tu Shan Li Lijuan Zhao Ran Xiao Xiuling Wang Fan Zhu | 2017 | Virologica Sinica2017，32，4： | 3 |
| 3 | Glucose metabolism controls humanγδ-cell-mediated tumor immunosurveillance in diabetes显示文摘Patients with type 2 diabetes mellitus(T2DM)have an increased risk of cancer.The effect of glucose metabolism onγδT cells and their impact on tumor surveillance remain unknown.Here,we showed that high glucose induced Warburg effect type of bioenergetic profle in Vy9vδ2 T cells,leading to excessive lactate accumulation,which further inhibited lytic granule secretion by impairing the traffcking of cytolytic machinery to the Vy9vδ2 T-cell-tumor synapse by suppressing AMPK activation and resulted in the loss of antitumor activity in vitro,in vivo and in patients.Strikingly,activating the AMPK pathway through glucose control or metformin treatment reversed the metabolic abnormalities and restored the antitumor activity of Vy9vδ2 T cells.These results suggest that the impaired antitumor activity of Vy9vδ2 T cells induced by dysregulated glucose metabolism may contribute to the increased cancer risk in T2DM patients and that metabolic reprogramming by targeting the AMPK pathway with metformin may improve tumor immunosurveillance. | Xiaofeng Mu Zheng Xiang Yan Xu Jing He Jianwen Lu Yuyuan Chen Xiwei Wang Chloe Ran Tu Yanme Wenyue Zhang Zhinan Yin Wing-hang Leung Yu-Lung Lau Yinping Liu Wenwei Tu | 2022 | Cellular & Molecular Immunology2022，19，8： | 2 |
| 4 | The influence of low atmospheric pressure on carbon monoxide of nheptane pool fires 显示文摘 | Fang Jun Yu Chunyu Tu Ran | 2008 | Journal of Hazardous Materials2008，154，123： | 1 |
| 5 | The influence of low atmospheric pressure on carbon monoxide of n-heptane pool fires 显示文摘 | Fang Jun Yu Chunyu Tu Ran | 2008 | Journal of Hazardous Materials2008，154，123： | 1 |
| 6 | Influence of low air pressure on combustion characteristics and flame pul- sation frequency of pool fires 显示文摘 | Fang Jun Tu Ran Guan Jinfu | 2011 | Fuel2011，90，8： | 1 |
| 7 | The influence of low atmospheric pressure on carbon monoxide of nheptane pool fires 显示文摘 | Fang Jun Chun Yuyu Tu Ran | 2008 | Journal of Hazardous Materials2008，154，123： | 1 |
| 8 | The prognostic value of circulating plasma DNA level and its allelic imbalance on chromosome 8p in patients with hepatocellutar carcinoma显示文摘 | Ran N Qin LX Tu H | 2006 | J Cancer Rcs Clin On- col2006，132，6： | 1 |
| 9 | PAS domain of the deduced Org35 protein mediates the interaction with NifA显示文摘NifA in Azospirillum brasilense plays a key role in regulating the synthesis of nitrogenase in response to ammonia and oxygen available. Recently, our laboratory has identified four clones, whose gene prodcuts interact with NifA, from A. brasilense Sp7 genomic libraries by using the yeast two-hybrid sys- tem with NifA as bait. We are interested in clone S35, one of the four clones, because it contains a PAS-domain coding region. The entire open reading frame (ORF) for the PAS domain-containing protein was isolated and designated as org35 here. org35 gene is 2211-bp long and encodes a protein of 736 aa with a predicted molecular weight of about 78.4 kD. The predicted amino acid sequence of org35 has similarity to some two-component sensor kinase/response regulator hybrids of bacteria. Struc- tural analyses showed that Org35 comprises at least three discrete conserved domains: the N-terminal PAS, the central histidine protein kinase (HPK) and the C-terminal response regulator (RR). The PAS domain of the deduced Org35 protein was found to interact directly with NifA, but the central HPK and the C-terminal RR domains of Org35 were not. These results indicated that interaction between NifA and Org35 was mediated by PAS domain. | TU Ran CUI Yanhua CHEN Sanfeng LI Jilun | 2006 | Chinese Science Bulletin2006，51，9： | 1 |
| 10 | Succession of bacterial community during composting:dissimilarity between compost mixture and biochar additive显示文摘Previous research showed that biochar addition facilitated composting and elevated nutrient retention.However,few of these studies explored bacterial structure and abundance in the compost mixture and biochar additive.Thus,this study aims to distinguish bacterial communities in both compost and bamboo biochar(BB)additive.Results indicated that the dynamics of nutrient contents in compost and BB samples were in a similar pattern,although there were lower levels of nutrients and metals(i.e.,Cu and Zn)in BB additives.The total number of operational taxonomic units(OTUs)in both compost and BB additives peaked on day 7 and then gradually reduced during composting.There was more abundance of bacteria in compost,whereas the diversity of bacteria was more in BB additives.Furthermore,the dominant bacteria in compost and BB samples were distinct at the different stages of composting.The Firmicutes steadily decreased in compost samples(from 34.78%to 7.65%),while it was the dominant phylum in BB additives during the whole composting period.Furthermore,Ruminofilibacter,Pseudoxanthomonas,and Actinomadura were the most abundant genera in compost samples than Pseudoxanthomonas,Azoarcus,and Paenibacillus in BB additives at the final stage of composting.Results from this study could provide a theoretical reference for the sound performance of biochar-added composting. | Muyuan Zhang Weng Liang Zhineng Tu Ronghua Li Zengqiang Zhang Amjad Ali Ran Xiao | 2021 | Biochar2021，3，2： | 1 |
| 11 | The influence of low atmospheric pressure on carb on monoxide of n-heptan epool fires显示文摘 | Fang Jun Yu Chun-Yu Tu Ran | 2008 | Journal of Hazardous Materials2008，154，： | 1 |
| 12 | The influence of low atmospheric pressure on carbon monoxide of n-heptane pool fires显示文摘 | Fang Jun Yu Chunyu Tu Ran | 2008 | Journal of Hazardous Materials2008，154，123： | 1 |
| 13 | The Influence of Low Atmospheric Pressure on Carbon Monoxide of n - heptane Pool Fires 显示文摘 | Fang Jun Chun yu Yu Tu Ran | 2008 | Journal of Hazardous Materials2008，，154： | 1 |
| 14 | The leakage behavior of supercritical CO2 flow in an experimental pipeline system 显示文摘 | Xie Qiyuan Tu Ran Jiang Xi Li Kang Zhou Xuejin | 2014 | Applied Energy2014，130，： | 1 |
| 15 | Effect of aminophylline on bispectral index 显示文摘 | TU RAN A MEMIS D KARAMANLYODTHLU B | 2004 | Acta Anaesthesiol Scand2004，48，： | 1 |
| 16 | Experimental demonstration of suppressing residual geometric dephasing显示文摘The geometric phase is regarded as a promising strategy in fault tolerance quantum information processing(QIP) domain due to its phase only depending on the geometry of the path executed. However, decoherence caused by environmental noise will destroy the geometric phase. Traditional dynamic decoupling sequences can eliminate dynamic dephasing but can not reduce residual geometric dephasing, which is still vital for high-precision quantum manipulation. In this work, we experimentally demonstrate effective suppression of residual geometric dephasing with modified dynamic decoupling schemes,using a single trapped171 Ybtion. The experimental results show that the modified schemes can reduce dephasing rate up to more than one order of magnitude compared with traditional dynamic decoupling schemes, where residual geometric dephasing dominates. Besides, we also investigate the impact of intensity and correlation time of the low-frequency noise on coherence of the quantum system. And we confirm these methods can be used in many cases. | Jin-Ming Cui Ming-Zhong Ai Ran He Zhong-Hua Qian Xiao-Ke Qin Yun-Feng Huang Zheng-Wei Zhou Chuan-Feng Li Tao Tu Guang-Can Guo | 2019 | Science Bulletin2019，64，23： | 1 |
| 17 | The influence of lowatmospheric pressure on carbon monoxide of n-heptanepool fires显示文摘 | Fang Jun Yu Chunyu Tu Ran | 2008 | Journal of Hazardous Materials2008，154，： | 1 |
| 18 | Designer cell-self-implemented labeling of microvesicles in situ with the intracellular-synthesized quantum dots显示文摘Cell-derived microvesicles(MVs) are secreted from almost all kinds of mammalian cells into the extracellular space, and play crucial roles in intercellular communication and transporting biomolecules between cells. However, there is a great challenge for visualizing and monitoring of MVs’ bio-behaviors due to the limitations of existing labeling methods. Herein, we report the first paradigm of designer cell-self-implemented labeling of MVs secreted from living mammalian MCF-7 cells in situ using the intracellular-synthesized fluorescent quantum dots(QDs) during the formation of MVs. By elaborately coupling intracellular biochemical reactions and metabolism pathways, the MCF-7 cells can be illuminated brightly by intracellular-biosynthesized fluorescent CdSe QDs. Simultaneously, intracellular-synthesized QDs can be in situ encapsulated by the secreted MVs budding from the plasma membrane of the fluorescing cells to label the MVs with an efficiency of up to 89.9%. The whole labeling process skillfully combines designer precise cell-tuned intricate synthesis of CdSe QDs with mild in-situ labeling via cell-selfimplementation just after feeding the cell with suitable chemicals, which is structure-or function-nondestructive and much more straightforward and milder than those by chemical conjugation or indirect encapsulation with conventional fluorogenic labels. | Ling-Hong Xiong Jia-Wei Tu Ya-Nan Zhang Ling-Ling Yang Ran Cui Zhi-Ling Zhang Dai-Wen Pang | 2020 | Science China Chemistry2020，63，4： | 1 |
| 19 | Selenium al ters t he lipid content and protein profile or rat hear t : An FTIR microspectroscopic study 显示文摘 | TO YRAN N TU RAN B SEV ERCAN F | 2007 | Arch Biochem Biophys2007，458，2： | 1 |
| 20 | Chloroquine enhances the efficacy of chemotherapy drugs against acute myeloid leukemia by inactivating the autophagy pathway显示文摘Current therapy for acute myeloid leukemia (AML) is largely hindered by the development of drug resistance of commonly used chemotherapy drugs, including cytarabine, daunorubicin, and idarubicin. In this study, we investigated the molecular mechanisms underlying the chemotherapy drug resistance and potential strategy to improve the efficacy of these drugs against AML. By analyzing data from ex vivo drug-response and multi-omics profiling public data for AML, we identified autophagy activation as a potential target in chemotherapy-resistant patients. In THP-1 and MV-4-11 cell lines, knockdown of autophagy-regulated genes ATG5 or MAP1LC3B significantly enhanced AML cell sensitivity to the chemotherapy drugs cytarabine, daunorubicin, and idarubicin. In silico screening, we found that chloroquine phosphate mimicked autophagy inactivation. We showed that chloroquine phosphate dose-dependently down-regulated the autophagy pathway in MV-4-11 cells. Furthermore, chloroquine phosphate exerted a synergistic antitumor effect with the chemotherapy drugs in vitro and in vivo. These results highlight autophagy activation as a drug resistance mechanism and the combination therapy of chloroquine phosphate and chemotherapy drugs can enhance anti-AML efficacy. | Han-lin Wang Jia-nan Li Wei-juan Kan Gao-ya Xu Guang-hao Luo Ning Song Wen-biao Wu Bo Feng Jing-feng Fu Yu-tong Tu Min-min Liu Ran Xu Yu-bo Zhou Gang Wei Jia Li | 2023 | Acta Pharmacologica Sinica2023，44，11： | 0 |
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