|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | The antiviral and antimicrobial activities of licorice, a widely-used Chinese herb显示文摘Licorice is a common herb which has been used in traditional Chinese medicine for centuries.More than 20 triterpenoids and nearly 300 flavonoids have been isolated from licorice. Recent studies have shown that these metabolites possess many pharmacological activities, such as antiviral,antimicrobial, anti-inflammatory, antitumor and other activities. This paper provides a summary of the antiviral and antimicrobial activities of licorice. The active components and the possible mechanisms for these activities are summarized in detail. This review will be helpful for the further studies of licorice for its potential therapeutic effects as an antiviral or an antimicrobial agent. | Liqiang Wang Rui Yang Bochuan Yuan Ying Liu Chunsheng Liu | 2015 | Acta Pharmaceutica Sinica B2015，5，4： | 64 |
| 2 | Antisense MMP-9 RNA inhibits malignant glioma cell growth in vitro and in vivo显示文摘The matrix-degrading metalloproteinases (MMPs), particularly MMP-9, play important roles in the pathogenesis and development of malignant gliomas. In the present study, the oncogenic role of MMP-9 in malignant glioma cells was investigated via antisense RNA blockade in vitro and in vivo. TJ905 malignant glioma cells were transfected with pcDNA3.0 vector expressing antisense MMP-9 RNA (pcDNA-AS-MMP9), which significantly decreased MMP-9 expression, and cell proliferation was assessed. For in vivo studies, U251 cells, a human malignant glioma cell line, were implanted subcutaneously into 4-to 6-week-old BALB/c nude mice. The mice bearing well-established U251 gliomas were treated with intratumoral pcDNA-AS-MMP9-Lipofectamine complex (AS-MMP-9-treated group), subcutaneous injection of endostatin (endostatin-treated group), or both (combined therapy group). Mice treated with pcDNA (empty vector)-Lipofectamine served as the control group. Four or eight weeks later, the volume and weight of tumor, MMP-9 expression, microvessel density and proliferative activity were assayed. We demonstrate that pcDNA-AS-MMP9 significantly decreased MMP-9 expression and inhibited glioma cell proliferation. Volume and weight of tumor, MMP-9 expression, microvessel density and proliferative activity in the antisense-MMP-9-treated and therapeutic alliance groups were significantly lower than those in the control group. The results suggest that MMP-9 not only promotes malignant glioma cell invasiveness, but also affects tumor cell proliferation. Blocking the expression of MMP-9 with antisense RNA substantially suppresses the malignant phenotype of glioma cells, and thus can be used as an effective therapeutic strategy for malignant gliomas. | Cuiyun Sun Qian Wang Hongxu Zhou Shizhu Yu Alain R. Simard Chunsheng Kang Yanyan Li Yanling Kong Tongling An Yanjun Wen Fudong Shi Junwei Hao | 2013 | Neuroscience Bulletin2013，29，1： | 14 |
| 3 | Growth and nutrient dynamics of Betula alnoides seedlings under exponential fertilization显示文摘Betula alnoides is a fast-growing hardwood species grown in large plantations in Southeast Asia and South China.Nitrogen requirements for producing robus seedlings,growth and nutrient dynamics were investigated using exponential fertilization treatments.Root collar diameter,height,dry mass and nutrient contents of seedlings increased exponentially in all fertilization treatments as time progressed.Moreover,with water soluble fertilizer(Plant Products plus microelements N–P_2O_5–K_2O:20–20–20),300 mg N seedling^(-1)was adequate.Vector analysis revealed that P was the most responsive nutrient element followed by N and K.Dilutions of N and K were evident in the plants without N addition,which induced initial P sufficiency and then luxury consumption probably due to the antagonistic interaction between N and P.However deficiencies of N,P and K were mostly observed in al exponential regimes during the experiment because seedling growth rate exceeded nutrient uptake rate,inferring that further study on improving the nutrient uptake efficiency is needed.Analysis of relationships among nutrient supply,dry mass,N content and N concentration demonstrated that 100–400 mg N seedling^(-1)induced sufficiency to luxury consumption of nitrogen without significant change in dry mass,and 400 mg N seedling^(-1)is recommended to apply for nutrient loading of seedlings before outplanting.The findings will help improve seedling quality and enhance the production of robust seedlings for plantation forestry of this species. | Lin Chen Chunsheng Wang Bernard Dell Zhigang Zhao Junjie Guo Daping Xu Jie Zeng | 2018 | Journal of Forestry Research2018，29，1： | 14 |
| 4 | Study on the Control of Tobacco Black Shank by Using Dry Mycelium of Penicillium Chrysogenum显示文摘 | Changliang Xu Rong Zeng Chunsheng Ruan Xiaoxia Wang Kaibao Xia George McBride Jianpeng Luo Suiyun Chen | 2010 | Journal of Life Sciences2010，4，1： | 12 |
| 5 | Micro-sprinkling irrigation simultaneously improves grain yield and protein concentration of winter wheat in the North China Plain显示文摘Increased grain yield(GY) and grain protein concentration(GPC) are the two main targets of efforts to improve wheat(Triticum aestivum L.) production in the North China Plain(NCP). We conducted a three-year field experiment in the 2014–2017 winter wheat growing seasons to compare the effects of conventional irrigation practice(CI) and micro-sprinkling irrigation combined with nitrogen(N) fertilizer(MSI) on GY, GPC, and protein yield(PY). Across the three years, GY, GPC, and PY increased by 10.5%–16.7%, 5.4%–8.0%, and 18.8%–24.6%, respectively, under MSI relative to CI. The higher GY under MSI was due primarily to increased thousand-kernel weight(TKW). The chlorophyll content of leaves was higher under MSI during the mid–late grain filling period, increasing the contribution of post-anthesis dry matter accumulation to GY, with consequent increases in total dry matter accumulation and harvest index compared to CI. During the mid–late grain filling period, the canopy temperature was markedly lower and the relative humidity was higher under MSI than under CI. The duration and rate of filling during the mid–late grain filling period were also higher under MSI than CI, resulting in higher TKW. MSI increased the contribution of post-anthesis N accumulation to grain N but reduced the pre-anthesis remobilization of N in leaves, the primary site of photosynthetic activity, possibly helping maintain photosynthate production in leaves during grain filling. Total N at maturity was higher under MSI than CI,although there was little difference in N harvest index. The higher GPC under MSI than under CI was due to a larger increase in grain N accumulation than in GY. Overall, MSI simultaneously increased both GY and GPC in winter wheat grown in the NCP. | Jinpeng Li Zhimin Wang Chunsheng Yao Zhen Zhang Yang Liu Yinghua Zhang | 2021 | The Crop Journal2021，9，6： | 11 |
| 6 | In-plane corrugated cosine honeycomb for 1D morphing skin and its application on variable camber wing显示文摘A novel 0-Poisson's ratio cosine honeycomb support structure of flexible skin is proposed. Mechanical model of the structure is analyzed with the energy method, finite element method (FEM) and experiments have been performed to validate the theoretical model. The in-plane characteristics of the cosine honeycomb are compared with accordion honeycomb through analytical models and experiments. Finally, the application of the cosine honeycomb on a variable camber wing is studied. Studies show that mechanical model agrees well with results of FEM and experiments. The transverse non-dimensional elastic modulus of the cosine honeycomb increases (decreases) when the wavelength or the wall width increases (decreases), or when the amplitude decreases (increases). Compared with accordion honeycomb, the transverse non-dimensional elastic modulus of the cosine honeycomb is smaller, which means the driving force is smaller and the power consumption is less during deformation. In addition, the cosine honeycomb can satisfy the deform- ing requirements of the variable camber wing. | Liu Weidong Zhu Hua Zhou Shengqiang Bai Yalei Wang Yuan Zhao Chunsheng | 2013 | Chinese Journal of Aeronautics2013，26，4： | 10 |
| 7 | Endogenously produced FGF2 is essential for the survival and proliferation of cultured mouse spermatogonial stem cells显示文摘 | Yan Zhang Si Wang Xiuxia Wang Shangying Liao Yujian Wu Chunsheng Han | 2012 | Cell Research2012，22，4： | 10 |
| 8 | ^(40)Ar/^(39)Ar ages of seamount trachytes from the South China Sea and implications for the evolution of the northwestern sub-basin显示文摘A chronological study of seamount rocks in the South China Sea basin provides a great opportunity to understand the expansion and evolution history of the sea basin.In this paper,we analyzed the40Ar/39Ar age of trachytic samples collected from the Shuangfeng seamounts in the northwestern sub-basin of the South China Sea.The two samples yielded plateau ages of 23.80 0.18 and 23.29 0.22 Ma,respectively,which indicate magmatic activity in late Oligocene which helpful constraints the expansion time of the northwest sub-basin.Previous studies suggested that the northwestern sub-basin and southwestern sub-basin have experienced a relatively consistent expansion in the NWeSE direction followed by a late expansion of the eastern sub-basin.We concluded that the expansion of the northwestern sub-basin began prior to ca.24 Ma,which also implicated magmatic events of a late or stop expansion of the northwestern sub-basin combined with our results of40Ar/39Ar age data and previous geophysical data. | Xiaohu Li Jiabiao Li Xing Yu Chunsheng Wang Fred Jourdan | 2015 | Geoscience Frontiers2015，6，4： | 9 |
| 9 | Chronic intermittent hypoxia accelerates liver fibrosis in rats with combined hypoxia and nonalcoholic steatohepatitis via angiogenesis rather than endoplasmic reticulum stress显示文摘In the present study,we aimed to investigate the role of endoplasmic reticulum stress(ERS)and its related inflammation and angiogenesis in liver fibrosis in a rat model of combined hypoxia and nonalcoholic steatohepatitis(NASH)and to confirm whether the intervention of hypoxia-inducible factor 1α(HIF1α)can improve fibrosis.Liver histological changes and biochemical indices,HIF1α,inflammatory factors,ERS-related parameters(GRP78,CHOP,caspase-3,and caspase-12),and angiogenesis indices(VEGFA,VEGFR2,and CD34)were evaluated.Compared with the control rats,the liver tissue of rats with hypoxia and NASH had obvious NASH characteristics and hepatic fibrosis was significantly aggravated,including bridging fibrosis in some rats.The mRNA expression levels of HIF1α,VEGFA,and VEGFR2 and total immunohistochemical staining scores of VEGFR2 and CD34 were significantly increased.In addition,HIF1α silencing significantly decreased HIF1α,biochemical indices(ALT,AST,and TG),inflammatory factors(TNFα,IL6,and IL1β),and angiogenesis indices(CD34 and VEGFR2),consequently,improved the hepatic fibrosis score in the rat model of combined hypoxia and NASH.Taken together,chronic intermittent hypoxia accelerates liver fibrosis in rats with combined hypoxia and NASH via angiogenesis rather than ERS and HIF1α intervention can improve liver fibrosis,angiogenesis,inflammatory factors,and biochemical indices.Therefore,HIF1α is a key regulatory factor of liver fibrosis in rats with combined hypoxia and NASH. | Wei Wu Weiping Li Jiaojiao Wei Chunsheng Wang Yunliang Yao Weihua Zhu Weimei He Weimei Zhou Jiang Liu | 2019 | Acta Biochimica et Biophysica Sinica2019，51，2： | 8 |
| 10 | Impending HRT wave precursors to the Wenchuan M_s8.0 earthquake and methods of earthquake impending prediction by using HRT wave显示文摘We deployed four geo-electric monitoring stations in Sichuan and Yunnan provinces from 2004, using the new generation of equipment (PS-100) and technologies to capture the HRT wave earthquake precursor. Before the Wenchuan Ms8.0 earthquake, we recorded the HRT wave precursor at the only operating station in Hongge (HG, Δ=465 km) and found that significant impending signal had been recorded at the station in the early morning ( 0―5 am) of 12th of May, 2008. The precursor for this earthquake is consistent with precursors recorded for other strong earthquakes. The measured physical properties (geo-resistivity and telluric-current) show tidal wave period oscillations from several days to several months before the earthquakes and the amplitude of such HT oscillation increases significantly towards the occurrence of an earthquake. These HT and RT waves from the epicenter have a causal relationship with the earthquakes that happened several days later. The arrival time of two RT waves is proportional to the distance from the station to the epicenter. The estimated natural decay of the amplitude is correlated with the natural period (T0) of the earthquake fault, which is proportional to the fault length. From this relationship, we can predict the earthquake magnitude. For magnitude 6―9 earthquakes, the natural period is about 1―6 hours. Such oscillation comes from the epicenter area and they can propagate several thousand kilometers in the Earth's crust. Before a strong earthquake in the shallow crust, the conductive pore fluid will experience major changes before the fault rapture. Such fluid change will emit an oscillation in the pore fluid pressure. This is the mechanism for the HRT wave generation. Since the China Earthquake Administration funded the HRT wave short-term earth-quake prediction project in 2003, the first record of HRT precursor wave has been recorded from the 2004-12-26 Sumatra Mw9.0 earthquake with the largest epicentre distance Δ=2900 km. Thereafter, we have captured HRT waves from more than twenty strong earthquakes, which are well-matched and show repeatability, consistency and regularity. All our observation with the HRT waves demonstrate that HRT wave precursors to earthquakes indeed exist. Strong earthquakes can be predicted and short-term and impending earthquake prediction is achievable in the very near future. From all the observations, including the ones at HG station from Wenchuan Ms8.0 earthquake, we conclude that using HRT wave to predict earthquakes is feasible. | QIAN FuYe ZHAO BiRu QIAN Wei ZHAO Jian HE ShiGen ZHANG HongKui LI ShiYu LI ShaoKun YAN GuLiang WANG ChengMin SUN ZhenKai ZHANG DongNing LU Jun ZHANG Ping YANG GuoJun SUN JiaLin GUO ChunSheng TANG YuXiong XU JianMing XIA KunTao JU Hang YIN BangHong LI Ming YANG DongSheng QI WeiLuo HE TaiMing GUAN HuaPing ZHAO YuLin | 2009 | Science China Earth Sciences2009，52，10： | 8 |
| 11 | Investigation of Myocardial Stunning after Cardiopulmonary Resuscitation in Pigs显示文摘Objective To investigate cardiac function and myocardial perfusion during 48 h after cardiopulmonary resuscitation (CPR), further to test myocardial stunning and seek indicators for long‐term survival after CPR. Methods After 4 min of untreated ventricular fibrillation, fifteen anesthetized pigs were studied at baseline and 2 h, 4 h, 24 h, and 48 h after restoration of spontaneous circulation (ROSC). Hemodynamic data, echocardiography and gated‐single photon emission computed tomography myocardial perfusion images were carried out. Results Mean arterial pressure (MAP), coronary perfusion pressure (CPP) and cardiac troponin I (CTNI) showed significant differences between eventual survival animals and non‐survival animals at 4 h after ROSC (109.2±10.7 mmHg vs. 94.8±12.3 mmHg, P=0.048; 100.8±6.9 mmHg vs. 84.4±12.6 mmHg, P=0.011; 1.60±0.13 ug/L vs. 1.75±0.10 ug/L, P=0.046). Mitral valve early‐to‐late diastolic peak velocity ratio, mitral valve deceleration time recovered 24 h; ejection faction and the summed rest score recovered 48 h after ROSC. Conclusion Cardiac systolic and early active relaxation dysfunctions were reversible within survival animals; cardiac stunning might be potentially adaptive and protective after CPR. The recovery of MAP, CPP, and CTNI could be the indicators for long‐term survival after CPR. | YANGLin LI ChunSheng GAO ChunJin WANG Shuo JI XianFei SU ZhiYu | 2011 | Biomedical and Environmental Sciences2011，24，2： | 8 |
| 12 | Theoretical Analysis and Experimental Verification on Valve-less Piezoelectric Pump with Hemisphere-segment Bluff-body显示文摘Existing researches on no-moving part valves in valve-less piezoelectric pumps mainly concentrate on pipeline valves and chamber bottom valves,which leads to the complex structure and manufacturing process of pump channel and chamber bottom.Furthermore,position fixed valves with respect to the inlet and outlet also makes the adjustability and controllability of flow rate worse.In order to overcome these shortcomings,this paper puts forward a novel implantable structure of valve-less piezoelectric pump with hemisphere-segments in the pump chamber.Based on the theory of flow around bluff-body,the flow resistance on the spherical and round surface of hemisphere-segment is different when fluid flows through,and the macroscopic flow resistance differences thus formed are also different.A novel valve-less piezoelectric pump with hemisphere-segment bluff-body(HSBB)is presented and designed.HSBB is the no-moving part valve.By the method of volume and momentum comparison,the stress on the bluff-body in the pump chamber is analyzed.The essential reason of unidirectional fluid pumping is expounded,and the flow rate formula is obtained.To verify the theory,a prototype is produced.By using the prototype,experimental research on the relationship between flow rate,pressure difference,voltage,and frequency has been carried out,which proves the correctness of the above theory.This prototype has six hemisphere-segments in the chamber filled with water,and the effective diameter of the piezoelectric bimorph is 30mm.The experiment result shows that the flow rate can reach 0.50 mL/s at the frequency of 6 Hz and the voltage of 110 V.Besides,the pressure difference can reach 26.2 mm H2O at the frequency of 6 Hz and the voltage of 160 V.This research proposes a valve-less piezoelectric pump with hemisphere-segment bluff-body,and its validity and feasibility is verified through theoretical analysis and experiment. | JI Jing ZHANG Jianhui XIA Qixiao WANG Shouyin HUANG Jun ZHAO Chunsheng | 2014 | Chinese Journal of Mechanical Engineering2014，27，3： | 8 |
| 13 | Topology optimization of support structure of telescope skin based on bit-matrix representation NSGA-II显示文摘Non-dominated sorting genetic algorithm II(NSGA-II)with multiple constraints handling is employed for multi-objective optimization of the topological structure of telescope skin,in which a bit-matrix is used as the representation of a chromosome,and genetic algorithm(GA)operators are introduced based on the matrix.Objectives including mass,in-plane performance,and out-of-plane load-bearing ability of the individuals are obtained by fnite element analysis(FEA)using ANSYS,and the matrix-based optimization algorithm is realized in MATLAB by handling multiple constraints such as structural connectivity and in-plane strain requirements.Feasible confgurations of the support structure are achieved.The results confrm that the matrix-based NSGA-II with multiple constraints handling provides an effective method for two-dimensional multi-objective topology optimization. | Liu Weidong Zhu Hua Wang Yiping Zhou Shengqiang Bai Yalei Zhao Chunsheng | 2013 | Chinese Journal of Aeronautics2013，26，6： | 7 |
| 14 | 3D-QSAR study on atmospheric half-lives of POPs using CoMFA and CoMSIA显示文摘3D-QSAR studies of persistent organic pollutants(POPs)screening for atmosphere persistence were performed by comparative molecular field analysis(CoMFA)and comparative molecular similarity index analysis(CoMSIA)methods.The mean and maximum half-life estimations for degradation in air of 49 UNEP POPs and possible POPs were modeled.Both groups’data have been modeled to obtain an average estimate and a predictive value for ranking and screening purposes.CoMFA and CoMSIA models have given cross-validation regression coeffcient(q2)values of more than 0.69 and correlation coeffcient(r2)value of more than 0.84,which validated for their prediction,could be applied to predict unavailable data. | Yuyin LV Chunsheng YIN Hongyan LIU Zhongsheng YI Yang WANG | 2008 | Journal of Environmental Sciences2008，20，12： | 7 |
| 15 | Numerical simulation of gas-solid flow with two fluid model in a spouted-fluid bed显示文摘The flow characteristics in a spouted-fluid bed differ from those in spouted or fluidized beds because of the injection of the spouting gas and the introduction of a fluidizing gas.The flow behavior of gas-solid phases was predicted using the Eulerian-Eulerian two-fluid model(TFM) approach with kinetic theory for granular flow to obtain the flow patterns in spouted-fluid beds.The gas flux and gas incident angle have a significant influence on the porosity and particle concentration in gas-solid spouted-fluid beds.The fluidizing gas flux affects the flow behavior of particles in the fountain.In the spouted-fluid bed,the solids volume fraction is low in the spout and high in the annulus.However,the solids volume fraction is reduced near the wall. | Shuyan Wang Liqian Zhao Chunsheng Wang Yinsong Liu Jinsen Gao Yang Liu Qinglin Cheng | 2014 | Particuology2014，12，3： | 7 |
| 16 | COR27 and COR28 encode nighttime repressors integrating Arabidopsis circadian clock and cold response显示文摘It was noted that circadian components function in plant adaptation to diurnal temperature cycles and freezing tolerance.Our genome-wide transcriptome analysis revealed that evening-phased COR27 and COR28 mainly repress the transcription of clockassociated evening genes PRR5,ELF4 and cold-responsive genes.Chromatin immunoprecipitation indicated that CCA1 is recruited to the site containing EE elements of COR27 and COR28 promoters in a temperaturedependent way.Further genetic analysis shows COR28 is essential for the circadian function of PRR9 and PRR7.Together,our results support a role of COR27 and COR28 as nighttime repressors integrating circadian clock and plant cold stress responses. | peng wang xuan cui chunsheng zhao liyan shi guowei zhang fenglong sun xiaofeng cao li yuan qiguang xie xiaodong xu | 2017 | Journal of Integrative Plant Biology2017，59，2： | 6 |
| 17 | Laparoscopic treatment for suspected gallbladder cancer confined to the wall: a 10-year study from a single institution显示文摘Objective: Although laparoscopic treatment of gallbladder cancer(GBC) has been explored in the last decade,long-term results are still rare. This study evaluates long-term results of intended laparoscopic treatment for suspected GBC confined to the gallbladder wall, based on our experience over 10 years.Methods: Between August 2006 and December 2015, 164 patients with suspected GBC confined to the wall were enrolled in the protocol for laparoscopic surgery. The process for GBC treatment was analyzed to evaluate the feasibility of computed tomography(CT) and/or magnetic resonance imaging(MRI) combined with frozen-section examination in identifying GBC confined to the wall. Of 159 patients who underwent the intended laparoscopic radical treatment, 47 with pathologically proven GBC were investigated to determine the safety and oncologic outcomes of a laparoscopic approach to GBC.Results: Among the 164 patients, 5 patients avoided further radical surgery because of unresectable disease and12 were converted to open surgery; in the remaining 147 patients, totally laparoscopic treatment was successfully accomplished. Extended cholecystectomy was performed in 37 patients and simple cholecystectomy in 10. The T stages based on final pathology were Tis(n=6), T1 a(n=2), T1 b(n=9), T2(n=26), and T3(n=4). Recurrence was detected in 11 patients over a median follow-up of 51 months. The disease-specific 5-year survival rate of these 47 patients was 68.8%, and rose to 85% for patients with a normal cancer antigen 19-9(CA19-9) level.Conclusions: The favorable long-term outcomes demonstrate the feasibility of combined CT/MRI and frozensection examination in the selection of patients with GBC confined to the gallbladder wall, confirm the oncologic safety of laparoscopic treatment in selected GBC patients, and favor measurement of preoperative CA19-9 in the selection of GBCs suitable for laparoscopic treatment. | Lingfu Zhang Chunsheng Hou Zhi Xu Lixin Wang Xiaofeng Ling Dianrong Xiu | 2018 | Chinese Journal of Cancer Research2018，30，1： | 6 |
| 18 | Dynamic Simulation and Disturbance Torque Analyzing of Motional Cable Harness Based on Kirchhoff Rod Model显示文摘The motional payloads on stabilized platform must be linked by some cable harnesses with other immobile apparatus.During the operation of stabilized platform,these cable harnesses can create spring disturbance torque which is exerted on the stabilized platform and then reduce the stabilizing precision.None of current studies can deal with the spring disturbance torque problem.To analyze the spring disturbance toque,a dynamic thin rod model is presented for simulating the motional cable harness which is based on the Kirchhoff rod theorem and can consider the geometrically non-linear effects.The internal bending and torsion restoring torques are simulated and then a predictive analysis of the disturbance torque can be performed in motional cable routing design.This model is solved with differential quadrature method(DQM).By using zeros of the Chebyshev polynomial as the grid points,the arc-coordinate is discretized to obtain a set of ordinary differential equations in time domain which is solved by implied method to obtain the profile and internal force of cable harness.The accuracy of this model is validated by comparing the simulation results and the experiment results(both the spring force and the deformed profile of the motional cable harness).In the experiment,a special optical measuring instrument based on binocular vision is developed.The comparison of experimental and simulated results shows that the simulation model can represent the real motional cable harness well,and the spring disturbance force simulation results are precise enough for spring disturbance torque analysis.This study will be helpful to obtain an optimized motional cable harness layout design with small spring disturbance torque. | WANG Chunsheng NING Ruxin LIU Jianhua ZHAO Tao | 2012 | Chinese Journal of Mechanical Engineering2012，25，2： | 6 |
| 19 | Multiple locus genealogies and phenotypic characters reappraise the causal agents of apple ring rot in China显示文摘Apple ring rot inflicts severe economic losses in the main apple producing areas of East Asia.The causal agent of the disease has been variously identified as Macrophoma kuwatsukai,Physalospora piricola and Botryosphaeria berengeriana f.sp.piricola,although B.dothidea is currently the most widely accepted pathogen name.The taxonomic uncertainty has delayed research that is needed to manage effectively this destructive disease.In the present study,genealogical concordance phylogenetic species recognition(GCPSR)was applied to pathogenic fungal isolates from apple and pear from several locations in China,along with several reference isolates.Phylogenetic results based on sequences of four nuclear loci(ITS,EF-1α,HIS and HSP)revealed the existence of two species within the examined isolates.One includes an ex-epitype isolate of B.dothidea and the other includes an isolate that was previously designated as B.berengeriana f.sp.piricola.Morphologically,the latter taxon presented an appressed mycelial mat on PDA whereas B.dothidea displayed columns of aerial mycelia reaching the lids,and conidia of the latter species were longer than B.dothidea.Botryosphaeria dothidea had a faster growth rate than the latter taxon under relatively high temperatures.Pathogenicity tests showed that on pear stems the latter taxon caused large-scale cankers along with blisters whereas B.dothidea was non-pathogenic,but on apple shoots the two fungi induced large and small wart-like prominences,respectively.Overall,this cryptic species demonstrated sufficient genetic variations and biological differences from B.dothidea.As a result of taxonomic study,we described here the latter taxon in a new combination,Botryosphaeria kuwatsukai and designate an epitype.Both B.kuwatsukai and B.dothidea are considered to be the main causal agents for apple ring rot in China and Japan. | Chao Xu Chunsheng Wang Liangliang Ju Rong Zhang Alan R.Biggs Eiji Tanaka Bingzhi Li Guangyu Sun | 2015 | Fungal Diversity2015，，2： | 6 |
| 20 | Tension leg platform project execution显示文摘The first floating platform concept design work for South China Sea is undergoing in DMAR's office now. This tension leg platform has potential to become the first advanced floating production platform project. Project execution is always a challenge for floating system. This paper focuses on the critical elements of project execution for tension leg platform,and studies potential implications to future oil and gas exploration in South China Sea. There are many factors affecting successful execution of floating system project,including technical issues, engineering management,interface management,etc. There are also failure examples of project execution in the industry. The author has participated 28 large detailed projects and has gained extensive experience on floating projects,with ample hands-on project experiences. A detailed tension leg platform project study example and discussions in depth are presented for future project execution in China deepwater development. | Zhang Dagang Wang Chunsheng Zhang Tianyu | 2013 | Engineering Sciences2013，11，4： | 5 |
您所在的机构暂未开通此部分的功能，请联系管理员