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| 1 | Identification of HBsAg-specific antibodies from a mammalian cell displayed full-length human antibody library of healthy immunized donor显示文摘肝炎 B 免疫球蛋白(HBIG ) 在肝炎 B 的管理是重要的病毒(HBV ) 感染。试图作为 HBIG 的一种选择开发 recombinant monoclonal 抗体,我们报导 HBV 表面抗原(HBsAg ) 的成功的鉴定从在哺乳动物的房间上显示的一个全身的人的抗体图书馆的特定的抗体出现。把一个生来地使免疫的施主的外部血 mononuclear 房间的全部的 RNA 用作模板,抗体全部剧目被放大。联合四方法的结扎和 Flp 调停 recombinase 的集成(Flp 在里面) 系统,我们构造了每个房间在显示了一个种仅仅抗体分子的一个哺乳动物的基于房间的、充分人的、全身的抗体显示图书馆。由屏蔽使用激活荧光的房间排序的房间图书馆(FACS ) ,八个房间克隆显示那房间表面上的 HBsAg 特定的抗体被识别。抗体基因的 DNA 顺序分析揭示了三唯一的抗体。FACS 数据显示了那荧光灯表示(FSE ) 的力量,荧光灯绑定(FSB ) 的力量和相对有约束力的能力(RBA ) 都在他们之中是不同的。这些结果由使用我们的抗体表明了那哺乳动物的显示和屏蔽站台,我们能成功地从一个使免疫的全身的抗体图书馆识别抗原特定的抗体。因此,这个平台为治疗学的抗体的开发是很有用的。 | Chang-Zheng Li Zhong-Kun Liang Zhen-Rui Chen Hai-Bo Lou Ye Zhou Zhe-Huan Zhang Fei Yu Shuwen Liu Yuanping Zhou Shuguang Wu Wenling Zheng Wanlong Tan Shibo Jiang Chen Zhou | 2012 | Cellular & Molecular Immunology2012，9，2： | 7 |
| 2 | Clinicopathogenic studies of acute diarrhea in children显示文摘ClinicopathogenicstudiesofacutediarheainchildrenCAILiMin,ZHANGChang,CHENHe,JIANGWeiPingandMAOWenXiangDepartmentofInfectiou... | CAI Li Min, ZHANG Chang, CHEN He, JIANG Wei Ping and MAO Wen Xiang Department of Infectious Diseases, The First People′s Municipal Hospital of Wenling, Wenling City 317500, Zhejiang Province, China | 1997 | World Journal of Gastroenterology1997，3，3： | 2 |
| 3 | Neuropeptide Y gene transfection inhibits post-epileptic hippocampal synaptic reconstruction显示文摘Exogenous neuropeptide Y has antiepileptic effects; however, the underlying mechanism and optimal administration method for neuropeptide Y are still unresolved. Previous studies have used intracerebroventricular injection of neuropeptide Y into animal models of epilepsy. In this study, a recombinant adeno-associated virus expression vector carrying the neuropeptide Y gene was injected into the lateral ventricle of rats, while the ipsilateral hippocampus was injected with kainic acid to establish the epileptic model. After transfection of neuropeptide Y gene, mossy fiber sprouting in the hippocampal CA3 region of epileptic rats was significantly suppressed, hippocampal synaptophysin (p38) mRNA and protein expression were inhibited, and epileptic seizures were reduced. These experimental findings indicate that a recombinant adeno-associated virus expression vector carrying the neuropeptide Y gene reduces mossy fiber sprouting and inhibits abnormal synaptophysin expression, thereby suppressing post-epileptic synaptic reconstruction. | Fan Zhang Wenqing Zhao Wenling Li Changzheng Dong Xinying Zhang Jiang Wu Na Li Chuandong Liang | 2013 | Neural Regeneration Research2013，8，17： | 2 |
| 4 | Neuropeptide Y protects cerebral cortical neurons by regulating microglial immune function显示文摘Neuropeptide Y has been shown to inhibit the immunological activity of reactive microglia in the rat cerebral cortex, to reduce N-methyl-D-aspartate current(INMDA) in cortical neurons, and protect neurons. In this study, after primary cultured microglia from the cerebral cortex of rats were treated with lipopolysaccharide, interleukin-1β and tumor necrosis factor-α levels in the cell culture medium increased, and mRNA expression of these cytokines also increased. After primary cultured cortical neurons were incubated with the lipopolysaccharide-treated microglial conditioned medium, peak INMDA in neurons increased. These effects of lipopolysaccharide were suppressed by neuropeptide Y. After addition of the neuropeptide Y Y1 receptor antagonist BIBP3226, the effects of neuropeptide Y completely disappeared. These results suggest that neuropeptide Y prevents excessive production of interleukin-1β and tumor necrosis factor-α by inhibiting microglial reactivity. This reduces INMDA in rat cortical neurons, preventing excitotoxicity, thereby protecting neurons. | Qijun Li Changzheng Dong Wenling Li Wei Bu Jiang Wu Wenqing Zhao | 2014 | Neural Regeneration Research2014，9，9： | 1 |
| 5 | The first strain of monkeypox isolated in the Chinese Mainland and preserved at the National Pathogen Resource Center of China显示文摘On September 16,2022,the first imported monkeypox case was reported in the Chinese Mainland.Laboratory tests including nucleic acid detection were carried out in Chongqing Nan’an Centre for Disease Control and Prevention and Chongqing Center for Disease Control and Prevention.After that,monkeypox virus was isolated by the Institute for Viral Disease Control and Prevention and preserved at the National Pathogen Resource Center on September 18,2022.The National Pathogen Resource Center shared the basic information of monkeypox virus strain,samples,biosafety,strain imaging,RT-PCR primers,and probes sequence timely to support the prevention and control of monkeypox epidemic and facilitate the scientific research on vaccine development,drug screening and evaluation of monkeypox virus in the future. | Qiang Wei Baoying Huang Wei Huang Jingdong Song Li Mei Li Zhao Jing Yin Juan Zhang Wenling Wang Fei Ye Caiyun Li Liling Bai Yanhai Wang Yao Deng Bo Liu Mengnan Jiang Guizhen Wu Jun Han Hua Zhao Hua Ling Wenjie Tan Jianjun Liu | 2022 | Infectious Medicine2022，1，4： | 1 |
| 6 | Effects of Seaweed Extracts on Promoting Growth and Improving Stress Resistance in Sugarcane显示文摘A field investigation was executed in sugarcane producing area of Zhanjiang,Guangdong Province with YT03-373 as the test variety to understand the possible impacts of seaweed extracts on phenology,yield and quality attributes of sugarcane. Foliar application of seaweed extracts were carried out on sugarcane at seedling and tillering stages. Agronomic traits,yield,sugar content,leaf drought resistance and pest resistance of sugarcane were analyzed for optimization of application time at proper phenological stages. The results that compared to the control,the application of seaweed extracts at different stages of sugarcane enhanced production of sugarcane with increase rate of 10. 83%-12. 87%,and improved theoretical sugar yield of sugarcane up to 1. 9 t/ha while enhancing water retention capacity and water content of sugarcane leaves that consequently led to wilting risk reduction by escaping drought and improved resilience to drought impact on sugarcane. Seaweed extracts also performed excellent in pest management of sugarcane,and they exerted different control effects on sugarcane borers,aphids and thrips. An increase in output of sugarcane was observed to be 15. 09% compared to control,while the net income was improved by up to 32. 64% in the seaweed extracts application treatment. Foliar application of seaweed extracts not only enhanced growth,cold resistance,pest resistance,output value but also improved economic returns,giving a positive affirmation for the recommendation of seaweed extract application. | Diwen CHEN Ying HUANG Dachun SHEN Wenling ZHOU Junhua AO Yong JIANG Zhenrui HUANG Hassan Munir Fahd Rasul | 2019 | Asian Agricultural Research2019，11，4： | 1 |
| 7 | Identification of Low-level Faults in Dense Well Pattern by Joint Well-seismic Interpretation显示文摘 | Zhang Xin Gan Lideng Liu Wenling Jiang Yan | 2012 | 石油地球物理勘探2012，47，A02： | 0 |
| 8 | Profiles of SARS-CoV-2 RNA and Antibodies in Inpatients with COVID-19 not Related with Clinical Manifestation: A Single Centre Study显示文摘Dear Editor,A novel coronavirus disease(COVID-19)caused by acute respiratory syndrome coronavirus 2(SARS-Co V-2)first broke out in Wuhan,Hubei Province,China,in 2019.More than 50 million cases of COVID-19 have been confirmed globally with 1,280,868 deaths reported as of November12,2020(https://covid19.who.int/). | Li Zhao Ruqin Gao Roujian Lu Huijuan Wang Yao Deng Peihua Niu Fachun Jiang Baoying Huang Jiwei Liang Jing Jia Feng Zhang Wenling Wang Guizhen Wu Wenjie Tan | 2021 | Virologica Sinica2021，36，5： | 0 |
| 9 | RXRα agonist bexarotene attenuates radiation-induced skin injury by relieving oxidative stress显示文摘Objective: To investigate the protective effect of bexarotene in radiation-induced skin injury and elucidate underlying mechanism.Methods: Irradiated cellular and animal models were established using an X-ray linear accelerator. Cell viabilityand apoptosis were evaluated by CCK8 and flow cytometry. In vivo protective effect of bexarotene was measuredin irradiated SD rats. The antioxidant capacity of bexarotene was validated by DCF-DA method. The signalingpathways involved in bexarotene-mediated skin repair were enriched by RNA sequencing.Results: Bexarotene could significantly restore the proliferation and inhibit the apoptosis of WS1 cells with radiation damage (P < 0.05). Moreover, bexarotene could effectively shorten the process of skin damage andpromote skin repair in a rat model of radiation-induced skin injury (P < 0.05). Mechanistically, bexaroteneeffectively reduced the expression of RXRα (P < 0.05), thus leading to an early decrease in reactive oxygen species(ROS) after radiation exposure. Furthermore, a transcriptome analysis indicated that bexarotene-mediated recovery of radiation damage involves redox signaling, immune regulation, lipid metabolism and autophagy.Conclusion: Bexarotene is a promising therapeutic agent in the treatment of radiation-induced skin injury. | Sheng Jiang Weichao Cai Jianhui Chen Wenling Tu Yulan Liu Lixin Gong Yahui Feng Wei Mo Tao Yan Shuyu Zhang Daojiang Yu | 2022 | Radiation Medicine and Protection2022，3，2： | 0 |
| 10 | Two antibodies show broad,synergistic neutralization against SARS-CoV-2 variants by inducing conformational change within the RBD显示文摘Continual evolution of the severe acute respiratory syndrome coronavirus(SARS-CoV-2)virus has allowed for its gradual evasion of neutralizing antibodies(nAbs)produced in response to natural infection or vaccination.The rapid nature of these changes has incited a need for the development of superior broad nAbs(bnAbs)and/or the rational design of an antibody cocktail that can protect against the mutated virus strain.Here,we report two angiotensin-converting enzyme 2 competing nAbs—8H12 and 3E2—with synergistic neutralization but evaded by some Omicron subvariants.Cryo-electron microscopy reveals the two nAbs synergistic neutralizing virus through a rigorous pairing permitted by rearrangement of the 472-489 loop in the receptor-binding domain to avoid steric clashing.Bispecific antibodies based on these two nAbs tremendously extend the neutralizing breadth and restore neutralization against recent variants including currently dominant XBB.1.5.Together,these findings expand our understanding of the potential strategies for the neutralization of SARS-CoV-2 variants toward the design of broad-acting antibody therapeutics and vaccines. | Hui Sun Tingting Deng Yali Zhang Yanling Lin Yanan Jiang Yichao Jiang Yang Huang Shuo Song Lingyan Cui Tingting Li Hualong Xiong Miaolin Lan Liqin Liu Yu Li Qianjiao Fang Kunyu Yu Wenling Jiang Lizhi Zhou Yuqiong Que Tianying Zhang Quan Yuan Tong Cheng Zheng Zhang Hai Yu Jun Zhang Wenxin Luo Shaowei Li Qingbing Zheng Ying Gu Ningshao Xia | 2024 | Protein & Cell2024，15，2： | 0 |
| 11 | Research on China’s technology lists for addressing climate change显示文摘The compilation of technology lists addressing climate change has a guiding effect on promoting technological research and development,demonstration,and popularization.It is also crucial for China to strengthen ecological civilization construction,achieve the carbon emission peak and carbon neutrality target,and enhance global climate governance capabilities.This study first proposes the existing classification outline of the technology promotion lists,technology demand lists,and future technology lists.Then,different methodologies are integrated on the basis of the existing outline of four technology lists:China’s existing technological promotion list for addressing climate change,China’s demand list for climate change mitigation technology,China’s key technology list for addressing climate change,and China’s future technology list for addressing climate change.What’s more,core technologies are analyzed in the aspects of technology maturity,carbon reduction cost,carbon reduction potential,economic benefits,social influence,uncertainty,etc.The results show that:key industries and sectors in China already have relatively mature mitigation/adaptation technologies to support the achievement of climate change targets.The multi-sectoral system of promoting climate friendly technologies has been established,which has played an active role in tackling climate change.Currently,climate technology needs are concentrated in the traditional technology and equipment upgrading,renewable energy technology,and management decision-making support technology.The key technologies are concentrated in 3 major areas and 12 technological directions that urgently need a breakthrough.For carbon emmission peak and nentrality,carbon depth reduction and zero carbon emissions and geoengineering technology(CDR and SRM)have played an important role in forming the structure of global emissions and achieving carbon neutrality in the future.Thus,the uncertainty assessment for the comprehensive technology cost effectiveness,technology integration direction,technical maturity,ethics and ecological impacts is supportive to the national technology strategy.Finally,the presented study proposes several policy implications for medium-and long-term technology deployment,improving technology conversion rate,promoting the research and development of core technologies,and forming a technology list collaborative update and release mechanism. | Can Wang Jianhui Cong Ke Wang Yue Qi Wenjian Cai Yulong Li Sha Fu Wentao Wang Yuanyuan Wei Xinzhu Zheng Jiani Jiang Mingpeng Chen Wenling Liu Yongxiang Zhang Zhibing Tian Ji Chen Rui Li Haiqing Zuo | 2021 | Chinese Journal of Population,Resources and Environment2021，19，2： | 0 |
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