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| 1 | China Stroke Statistics 2019:A Report From the National Center for Healthcare Quality Management in Neurological Diseases,China National Clinical Research Center for Neurological Diseases,the Chinese Stroke Association,National Center for Chronic and Non-communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention and Institute for Global Neuroscience and Stroke Collaborations显示文摘China faces the greatest challenge from stroke in the world.The death rate for cerebrovascular diseases in China was 149.49 per 100000,accounting for 1.57 million deaths in 2018.It ranked third among the leading causes of death behind malignant tumours and heart disease.The age-standardised prevalence and incidence of stroke in 2013 were 1114.8 per 100000 population and 246.8 per 100000 person-years,respectively.According to the Global Burden of Disease Study 2017,the years of life lost(YLLs)per 100000 population for stroke increased by 14.6%;YLLs due to stroke rose from third highest among all causes in 1990 to the highest in 2017.The absolute numbers and rates per 100000 population for all-age disability-adjusted life years(DALYs)for stroke increased substantially between 1990 and 2017,and stroke was the leading cause of all-age DALYs in 2017.The main contributors to cerebrovascular diseases include behavioural risk factors(smoking and alcohol use)and pre-existing conditions(hypertension,diabetes mellitus,dyslipidaemia and atrial fibrillation(AF)).The most prevalent risk factors among stroke survivors were hypertension(63.0%-84.2%)and smoking(31.7%-47.6%).The least prevalent was AF(2.7%-7.4%).The prevalences for major risk factors for stroke are high and most have increased over time.Based on the latest national epidemiological data,26.6%of adults aged≥15 years(307.6 million adults)smoked tobacco products.For those aged≥18 years,age-adjusted prevalence of hypertension was 25.2%;adjusted prevalence of hypercholesterolaemia was 5.8%;and the standardised prevalence of diabetes was 10.9%.For those aged≥40 years,the standardised prevalence of AF was 2.31%.Data from the Hospital Quality Monitoring System showed that 3010204 inpatients with stroke were admitted to 1853 tertiary care hospitals during 2018.Of those,2466785(81.9%)were ischaemic strokes(ISs);447609(14.9%)were intracerebral haemorrhages(ICHs);and 95810(3.2%)were subarachnoid haemorrhages(SAHs).The average age of patients admitted was 66 years old,and nearly 60%were male.A total of 1555(0.1%),2774(0.6%)and 1347(1.4%)paediatric strokes(age<18 years)were identified among IS,ICH and SAH,respectively.Over one-third(1063892(35.3%))of the patients were covered by urban resident basic medical insurance,followed by urban employee basic medical insurance(699513(23.2%))and new rural cooperative medical schema(489361(16.3%)).The leading risk factor was hypertension(67.4%for IS,77.2%for ICH and 49.1%for SAH),and the leading comorbidity was pneumonia or pulmonary infection(10.1%for IS,31.4%for ICH and 25.2%for SAH).In-hospital death/discharge against medical advice rate was 8.3%for stroke inpatients,ranging from 5.8%for IS to 19.5%for ICH.The median and IQR of length of stay was 10.0(7.0-14.0)days,ranging from 10.0(7.0-13.0)in IS to 14.0(8.0-22.0)in SAH.Data from the Chinese Stroke Center Alliance demonstrated that the composite scores of guideline-recommended key performance indicators for patients with IS,ICH and SAH were 0.77±0.21,0.72±0.28 and 0.59±0.32,respectively. | Yong-Jun Wang Zi-Xiao Li Hong-Qiu Gu Yi Zhai Yong Jiang Xing-Quan Zhao Yi-Long Wang Xin Yang Chun-Juan Wang Xia Meng Hao Li Li-Ping Liu Jing Jing Jing Wu An-Ding Xu Qiang Dong David Wang Ji-Zong Zhao On behalf of China Stroke Statistics 2019 Writing Committee | 2020 | Stroke & Vascular Neurology2020，5，3： | 181 |
| 2 | Synthesis of graphitic carbon nitride through pyrolysis of melamine and its electrocatalysis for oxygen reduction reaction显示文摘Graphitic carbon nitride(g-C3N4) was synthesized via direct pyrolysis of melamine and its electrocatalysis toward oxygen reduction reaction was studied.The morphology and structures of the products were characterized by scanning electron microscope and X-ray powder diffractometer.It was found that higher pyrolysis temperature resulted in more perfect crystalline structure of the graphitic carbon nitride product.Electrochemical characterizations show that the g-C3N4 has electrocatalytic activity toward ORR through a two-step and two-electron process. | He-Sheng Zhai Lei Cao Xing-Hua Xia | 2013 | Chinese Chemical Letters2013，24，2： | 18 |
| 3 | Induced Resistance by the Toxin Filtrate of Bipolaris maydis Race T Cultivation显示文摘Resistance to maize southern leaf spot disease was induced by the low-concentration filtrate of Bipolaris maydis race T cultivation in an experiment. The nuclear neterogeny corn C103 was used as the test material. The lesion area on the leaves was significant difference by connalysis. The lesion areas on pretreated leaves were (0.3 ± 0.05) - (0.9 ± 0.5) mm2, but those on the control were (23.1 ± 8.7) mm2. At the same time, the changes in peroxidase, phenylalanine, ammonialyase, and malondialdehvde activities were determined. During 0-96 h of inspection, phenylalanine and ammonialyase (PAL) activities increased by 64.2%, peroxidase (POD) activities increased by 41.2%, but the malondialdenvde (MDA) content decreased by 29.7% compared with the control. It seems that the low-concentration filtrate of Bipolaris maydis race T cultivation itself can be used as an elicitor to enhance the induced resistance. | MA Chun-hong ZHAI Cai-xia WANG Li-an CHEN Xia LI Yun-chao GUO Xiu-lin CUI Si-ping LI Guang-min | 2006 | Agricultural Sciences in China2006，5，9： | 15 |
| 4 | Oral fibrinogen-depleting agent lumbrokinase for secondary ischemic stroke prevention: results from a multicenter, randomized, parallel- group and controlled clinical trial显示文摘 | Cao Yong-jun Zhang Xia Wang Wan-hua Zhai Wan-qing Qian Ju-fen Wang Jian-sheng Chen Jun You Nian-xing Zhao Zhong Wu Qiu-yi Xu Yuan Yuan Lei Li Rui-xia Liu Chun-feng | 2013 | Chinese Medical Journal2013，，21： | 13 |
| 5 | Trajectory tracking control of a VTOL unmanned aerial vehicle using offset-free tracking MPC显示文摘Designing a stable and robust flight control system for an Unmanned Aerial Vehicle(UAV)is an arduous task.This paper addresses the trajectory tracking control problem of a Ducted Fan UAV(DFUAV)using offset-free Model Predictive Control(MPC)technique in the presence of various uncertainties and external disturbances.The designed strategy aims to ensure adequate flight robustness and stability while overcoming the effects of time delays,parametric uncertainties,and disturbances.The six degrees of freedom DFUAV model is divided into three flight modes based on its airspeed,namely the hover,transition,and cruise mode.The Dryden wind turbulence is applied to the DFUAV in the linear and angular velocity component.Moreover,different uncertainties such as parametric,time delays in state and input,are introduced in translational and rotational components.From the previous work,the Linear Quadratic Tracker with Integrator(LQTI)is used for comparison to corroborate the performance of the designed controller.Simulations are computed to investigate the control performance for the aforementioned modes and different flight phases including the autonomous flight to validate the performance of the designed strategy.Finally,discussions are provided to demonstrate the effectiveness of the given methodology. | Tayyab MANZOOR Yuanqing XIA Di-Hua ZHAI Dailiang MA | 2020 | Chinese Journal of Aeronautics2020，33，7： | 10 |
| 6 | Hepatitis C virus core protein triggers expansion and activation of CD4+CD25+ regulatory T cells in chronic hepatitis C patients显示文摘 | Naicui Zhai Xiumei Chi Tianyang Li Hongxiao Song Haijun Li Xia Jin Ian Nicholas Crispe Lishan Su Junqi Niu Zhengkun Tu | 2015 | Cellular & Molecular Immunology2015，12，6： | 10 |
| 7 | Design and experimental study of the control system for precision seed-metering device显示文摘The precision seeding technique has been developed at full speed along with the continuous development of new agricultural technologies,especially those concerning cultivated patterns.The seed-metering device is the key component of a precision seeder.A ground wheel is used to drive the seed-metering device of the conventional direct seeder.However,the wheel bears high resistance and easily slips.Moreover,the adjustment of the precision seeder’s seeding rate is more difficult.In order to solve these problems,a control system which could keep the rotational speed of the seed-metering device consistent with the seeder’s working speed for the precision seed-metering device was designed.The control system includes a Hall sensor,a single chip microcomputer system,a motor control module,a stepper motor and a display.The control system used a Hall sensor to measure the seeder’s working speed and employed a single chip microcomputer system to predict the rotational speed of seed-metering device.It would then determine the relationship between the seeder’s working speed and the rotational speed of the seed metering-device according to the seeder’s working state,distance between seeds and the requirement of sowing rate.The system could effectively reduce the influence of inhomogeneous sowing caused by the ground wheel’s slipping.The system was found to be reliable by the experiment.The seeding control system could also make the speed of the seed-metering device and seeder’s uniform,improving the uniformity of the amount of seeding,and achieving the goal of design.This new design provides a platform to solve problems of the seed-metering device and the seeder. | Zhai Jianbo Xia Junfang Zhou Yong Zhang Shun | 2014 | International Journal of Agricultural and Biological Engineering2014，7，3： | 9 |
| 8 | Design of Embryo-electronic Systems Capable of Self-diagnosing and Self-healing and Configuration Control显示文摘空航天器工作于恶劣空间环境,要求其测控系统硬件具有高可靠性和自修复能力。胚胎电子系统是一种具有自检测、自修复能力的仿生硬件.根据多细胞生物体结构中自检测自修复机制,设计了包含DNA存储器、功能模块、状态控制和自诊断模块以及物理连接模块等的胚胎电子系统体系结构及其容错机制。又针对目前配置存储器无法满足大规模胚胎电子系统要求,提出了基于移位寄存器的胚胎电子系统配置存储器新结构及其配置控制技术。设计了基于移位寄存器的胚胎电子系统配置存储器的整体结构,整个胚胎电子系统的配置存储器连接在一起形成一个链式结构。并设计了配置存储器单元的内部电路以及基于移位寄存器的胚胎电子系统配置控制和动态重构方法。实验表明,该系统既能够实现胚胎电子系统的故障自修复和胚胎电子系统功能的在线动态重构,该存储器结构相比基于SRAM的配置存储器结构,在128×128以上的大规模胚胎电子系统时,可以节约大约90%以上的存储器面积。 | Xu Guili Xia Zhenghao Wang Haibin Zhang Zhai Wang Youren | 2009 | Chinese Journal of Aeronautics2009，22，6： | 9 |
| 9 | Mechanism of pulse magneto-oscillation grain refinement on pure Al显示文摘Pulse magneto-oscillation (PMO) was developed as a novel technique to refine the solidification structure of pure aluminium.Its grain refining mechanism was proposed.The PMO refinement mechanism is that the nucleus falls off from the mould wall and drifts into the melt under the action of PMO.The solidification structure of Al melt depends on the linear electric current density,and also the discharge and oscillation frequencies.The radial pressure of PMO sound wave is the major factor that contributes to the migration of nucleus into the melt. | Pei Ning Gong Yongyong Li Renxing Xia Zanqi Zhai Qijie | 2011 | China Foundry2011，8，1： | 8 |
| 10 | Determination of Electrical Conductivity of Cadaver Skeletal Muscle: A Promising Method for the Estimation of Late Postmortem Interval显示文摘The electrical conductivity(EC)of extracted muscle fluid has been extensively used to evaluate meat freshness and shelf life in the field of food sanitation for decades.The opposite of freshness is the corruption that increases with time.Based on the freshness/corruption principle,we investigated the relationship between long postmortem intervals(PMIs)and EC in cadaver skeletal muscle.EC values of extracted fluid from rat muscles were measured at different PMIs for 10 days.The results indicate that there was a significant correlation between PMI and EC,and the data fit well to the cubic polynomial regression equation y=‑0.01x3+0.264x2‑13.657x+1769.148(R2=0.925).In addition,the EC of different dilutions of these muscle extracts showed strict quadratic correlation(R2=1)with the dilution ratios,suggesting that EC can be measured with very small quantities of muscle sample.Our study suggests that determination of the EC of cadaver skeletal muscle extracts may be a useful method for estimating long PMIs. | Zhiyuan Xia Xiandun Zhai Beibei Liu Yaonan Mo | 2015 | Journal of Forensic Science and Medicine2015，1，1： | 8 |
| 11 | Characteristics of entropy generation and heat transfer in a microchannel with fan-shaped reentrant cavities and internal ribs显示文摘Three-dimensional laminar fluid flow and heat transfer in a micro heat sink with cavities and internal ribs are investigated us-ing numerical methods. Moreover, according to the second law of thermodynamics, the model of entropy generation is also established for variable cross section of the microchannel. The simulation encompasses Reynolds number of 198-600, relative cavity height e1/Dhvalues of 0-0.65, relative rib height e2/Dhrange of 0-0.2167. The results show that the effect of relative rib height on entropy generation is significant, while the relative cavity height has little effect on it. The combined effect of cavities and ribs in the microchannel has better performance of heat transfer than the smooth microchannel under similar conditions. Extensive simulations are conducted to collect data on the characteristics of heat transfer and fluid flow in a micro heat sink with cavities and internal ribs. Using these data, correlations for Nusselt number and friction factor in terms of Reynolds number and the geometry of cavity and rib have been developed. | XIA GuoDong ZHAI YuLing CUI ZhenZhen | 2013 | Science China(Technological Sciences)2013，56，7： | 8 |
| 12 | Successful Production of an All-Female Common Carp (Cyprinus carpio L.) Population Using cyp17a1-Deficient Neomale Carp显示文摘Due to sexual dimorphism in the growth of certain cultured fish species,the production of monosex fishes is desirable for the aquaculture industry.Nowadays,the most widely practiced technique available for the mass production of monosex fish populations is sex steroid-induced sex reversal.Here,a novel strategy for the successful production of all-female(AF)common carp(Cyprinus carpio L.),to take advantage of the sexual dimorphism in growth documented in this species,has been developed using genetic engineering via single gene-targeting manipulation without any exogenous hormone treatments.Male and female heterozygous cyp17a1-deficient common carp were first obtained using the clustered regularly interspaced short palindromic repeats/CRISPR-associated endonuclease 9(CRISPR/Cas9)technique.An all-male phenotype for homozygous cyp17a1-deficient carp,regardless of the individuals’sexdetermination genotypes(XY or XX),has been observed.A male-specific DNA marker newly identified in our laboratory was used to screen the neomale carp population with the XX genotype from the cyp17a1-deficient carp.These neomale carp develop a normal testis structure with normal spermatogenesis and sperm capacity.The neomale common carp were then mated with wild-type(WT)females(cyp17a1^(+/+)XX genotype)using artificial fertilization.All the AF offspring sample fish from the neomale-WT female mating were confirmed as having the cyp17a^(1+/-)XX genotype,and normal development of gonads to ovaries was observed in 100.00%of this group at eight months post-fertilization(mpf).A total of 1000 carp fingerlings,500 from the WT male and female and 500 from the neomale and WT female mating,were mixed and reared in the same pond.The average body weight of cyp17a1^(+/-)XX females was higher by 6.60%(8 mpf)and 32.66%(12 mpf)than that of the control common carp.Our study demonstrates the first successful production of a monosex teleost population with the advantages of sexual dimorphism in growth using genetic manipulation targeting a single locus. | Gang Zhai Tingting Shu Kuangxin Chen Qiyong Lou Jingyi Jia Jianfei Huang Chuang Shi Xia Jin Jiangyan He Donghuo Jiang Xueqiao Qian Wei Hu Zhan Yin | 2022 | Engineering2022，8，1： | 7 |
| 13 | Effect of Bushenhuoxue formula on interleukin-1 beta and discoidin domain receptor 2 levels in a rat model of osteoarthritis显示文摘OBJECTIVE:To determine the effects of Bushenhuoxue formula(BHF) on interleukin-1 beta(IL-1β),transforming growth factor beta 1(TGF-β1),discoidin domain receptor 2(DDR2) and matrix metalloproteinase-1(MMP-1) levels in a rat model of osteoarthritis(OA).METHODS:Sprague-Dawley rats were used to establish an OA model and subjected to various treatments over 6 weeks.Rats were treated with BHF,glucosamine sulfate(G5),or starch as a control.Serum levels of IL-1β and MMP-1 and joint fluid levels of IL-1 β were determined by means of ELISAs.We used immunohistochemistry to determine DDR2 levels in knee cartilage.Gene expression levels of MMP-1 in joint synovial tissue were assessed using reverse transcription polymerase chain reaction assays.RESULTS:Serum IL-1β levels were unchanged throughout the study.Levels of IL-1β in joint fluid and MMP-1 in sera from the BHF- and GS-treated groups were significantly reduced.DDR2 levels in knee cartilage were also significantly reduced in the BHF group.Expression of the MMP-1 gene was significantly reduced by BHF treatment.CONCLUSION:BHF might be beneficial in the inhibition and alleviation of local inflammatory responses and cartilage degeneration in OA. | Li Nianhu Xia Lei Yi Sheng Yu Jianhui Zhai Yi Xu Zhanwang | 2015 | Journal of Traditional Chinese Medicine2015，35，2： | 7 |
| 14 | Androgen signaling regulates the transcription of anti-Müllerian hormone via synergy with SRY-related protein SOX9A显示文摘Anti-Müllerian hormone(amh) is one of the earliest functional genes expressed during testicular differentiation. It has been suggested that androgen signaling regulates critical genes for the differentiation and development of the testis. To elucidate the exact regulatory mechanisms involved in amh transcription mediated by androgen signaling, androgen signaling was manipulated in zebrafish by cytochrome P45017a1(cyp17a1) knockout and Flutamide treatment. In cyp17a1-deficient and Flutamide-treated testes,up-regulated sry-box9a(sox9a) and down-regulated amh were observed. Moreover, a physical association of the zebrafish androgen receptor(AR) and SOX9 A was found. The interaction between AR and SOX9 A was mediated via the DNA binding domain(DBD) of AR and the transactivation domain(TA) of SOX9 A,and was further enhanced by 5-alpha dihydrotestosterone(DHT), one of the most potent androgens.Intriguingly, together with SOX9 A, androgen signaling synergistically promoted amh transcription,mainly through the proximal 1 kb of the amh promoter region. Taken together, our data demonstrate a critical mechanism underlying the direct synergy of androgen signaling and SOX9 A in the regulation of amh transcription. | Gang Zhai Tingting Shu Yuguo Xia Xia Jin Jiangyan He Zhan Yin | 2017 | Science Bulletin2017，62，3： | 7 |
| 15 | Delivery room resuscitation and short-term outcomes of extremely preterm and extremely low birth weight infants: a multicenter survey in North China显示文摘Background:Delivery room resuscitation assists preterm infants,especially extremely preterm infants(EPI)and extremely low birth weight infants(ELBWI),in breathing support,while it potentially exerts a negative impact on the lungs and outcomes of preterm infants.This study aimed to assess delivery room resuscitation and discharge outcomes of EPI and ELBWI in China.Methods:The clinical data of EPI(gestational age[GA]<28 weeks)and ELBWI(birth weight[BW]<1000 g),admitted within 72 h of birth in 33 neonatal intensive care units from five provinces and cities in North China between 2017 and 2018,were analyzed.The primary outcomes were delivery room resuscitation and risk factors for delivery room intubation(DRI).The secondary outcomes were survival rates,incidence of bronchopulmonary dysplasia(BPD),and risk factors for BPD.Results:A cohort of 952 preterm infants were enrolled.The incidence of DRI,chest compressions,and administration of epinephrine was 55.9%(532/952),12.5%(119/952),and 7.0%(67/952),respectively.Multivariate analysis revealed that the risk factors for DRI were GA<28 weeks(odds ratio[OR],3.147;95%confidence interval[CI],2.082–4.755),BW<1000 g(OR,2.240;95%CI,1.606–3.125),and antepartum infection(OR,1.429;95%CI,1.044–1.956).The survival rate was 65.9%(627/952)and was dependent on GA.The rate of BPD was 29.3%(181/627).Multivariate analysis showed that the risk factors for BPD were male(OR,1.603;95% CI,1.061–2.424),DRI(OR,2.094;95% CI,1.328–3.303),respiratory distress syndrome exposed to≥2 doses of pulmonary surfactants(PS;OR,2.700;95%CI,1.679–4.343),and mechanical ventilation≥7 days(OR,4.358;95% CI,2.777–6.837).However,a larger BW(OR,0.998;95% CI,0.996–0.999),antenatal steroid(OR,0.577;95%CI,0.379–0.880),and PS use in the delivery room(OR,0.273;95%CI,0.160–0.467)were preventive factors for BPD(all P<0.05).Conclusion:Improving delivery room resuscitation and management of respiratory complications are imperative during early management of the health of EPI and ELBWI. | Shuai-Jun Li Qi Feng Xiu-Ying Tian Ying Zhou Yong Ji Yue-Mei Li Shu-Fen Zhai Wei Guo Fang Zhang Rong-Xiu Zheng Hai-Ying He Xia Liu Jun-Yi Wang Hua Mei Hong-Yun Wang Hua Xie Chao-Mei Zeng Li Ma Ping-Ping Zhang Jin-Yu Li Xiao-Ying Wang Li-Hua Li Hong Cui Shu-Lan Yang Lu Chen Xiao-Hong Gu Yan-Ju Hu Sheng-Shun Que Li-Xia Sun Ming Yang Wen-Li Zhao Qiu-Yan Ma Hai-Juan Wang Jiu-Ye Guo | 2021 | Chinese Medical Journal2021，，13： | 7 |
| 16 | Further results on cloud control systems显示文摘This paper is devoted to further investigating the cloud control systems(CCSs). The benefits and challenges of CCSs are provided. Both new research results of ours and some typical work made by other researchers are presented. It is believed that the CCSs can have huge and promising effects due to their potential advantages. | Yuanqing XIA Yongming QIN Di-Hua ZHAI Senchun CHAI | 2016 | Science China(Information Sciences)2016，59，7： | 6 |
| 17 | A draft sequence of the rice (Oryza sativa ssp. indica) genome显示文摘The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development, and evolution, as well as information on many beneficial phenotypes of economic significance. Using a 'whole genome shotgun' approach, we have pro-duced a draft rice genome sequence of Oryza sativa ssp. in-dica, the major crop rice subspecies in China and many other regions of Asia. The draft genome sequence is constructed from over 4.3 million successful sequencing traces with an accumulative total length of 2214.9 Mb. The initial assembly of the non-redundant sequences reached 409.76 Mb in length, based on 3.30 million successful sequencing traces with a total length of 1797.4 Mb from an indica variant cultivar 93-11, giving an estimated coverage of 95.29% of the rice genome with an average base accuracy of higher than 99%. The coverage of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEM-BLER, a custom-designed software package | YU Jun, HU Songnian, WANG Jun,LI Songgang WONG Ka-Shu Gane, LIU Bin,DENG Yajun, DAI Li, ZHOU Yan,ZHANG Xiuqing, CAO Mengliang, LIU Jing,SUN Jiandong , TANG Jiabin, CHEN Yanjiong,HUANG Xiaobing, LIN Wei, YE Chen, TONG Wei,CONG Lijuan, GENG Jianing, HAN Yujun, LI Lin,LI Wei, HU Guangqiang, HUANG Xiangang,LI Wenjie, LI Jian, LIU Zhanwei, LI Long,LIU Jianping, Ql Qiuhui, LIU Jinsong, LI Li,WANG Xuegang, LU Hong, WU Tingling,ZHU Miao, Nl Peixiang, HAN Hua, DONG Wei,REN Xiaoyu, FENG Xiaoli, GUI Peng,LI Xianran, WANG Hao, XU Xin, ZHAI Wenxue,XU Zhao, ZHANG Jinsong, HE Sijie,ZHANG Jianguo, XU Jichen, ZHANG Kunlin,ZHENG Xianwu, DONG Jianhai, ZENG Wanyong,TAO Lin, CHEN Xuewei, HE Jun, LIU Daofeng,TIAN Wei, TIAN Chaoguang, XIA Hongai,LI Gang, GAO Hui, LI Ping, CHEN Wei ,WANG Xudong, ZHANG Yong, HU Jianfei,WANG Jing, LIU Song, YANG Jian,ZHANG Guangyu, XIONG Yuqing, LI Zhijie,MAO Long, ZHOU Chengshu, ZHU Zhen,CHEN Runsheng, HAO Bailin,ZHENG Weimou, CHEN Shouyi, QUO Wei,LI Guojie, LIU Siqi, HUANG Guyang,TAO Ming, WANG Jian, ZHU Lihuang,YUAN Longping& YANG HuanmingBeijing Genomics Institute/Center of Genomics & Bioinformatics, Chinese Academy of Sciences, Beijing 101300, China Hangzhou Genomics Institute/Institute of Bioinformatics of Zhejiang University/Key Laboratory of Bioinformatics of Zhejiang Province, Hangzhou 310007, China Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China National Hybrid Rice R & D Center, Changsha 410125, China Laboratory of Bioinformatics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China College of Life Sciences, Peking University, Beijing 100871, China Institute of Theoretical Physics, Chinese Academy of Sciences, Beijing 1Q0080, China Digital China Ltd., Beijing 100080, China Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100080, China Medical College, Xi’an Jiaotong University, Xi’an 710061, ChinaThese authors contributed equally to this work.Corresponding author.Corresponden | 2001 | Chinese Science Bulletin2001，46，23： | 6 |
| 18 | Plasmonically induced transparency in double-layered graphene nanoribbons显示文摘Near-field coupled plasmonic systems generally achieve plasmonically induced transparency(PIT) using only one-way bright–dark mode coupling. However, it is challenging to realize such well-designed devices,mainly because they depend significantly on the polarization direction. We exploit surface plasmons supported by two crossed layers of graphene nanoribbons(GNRs) to achieve dynamically tunable PIT, where each GNR operates as both the bright and dark modes simultaneously. The proposed PIT can result from either one-way bright–dark mode interactions or bidirectional bright–bright and bright–dark mode hybridized coupling when the polarization is perpendicular/parallel or at an angle to the GNRs, respectively.Additionally, identical ribbon widths yield polarization-insensitive single-window PIT, whereas different ribbon widths produce polarization-dependent double-window PIT. We examine the proposed technique using plasmon wave functions and the transfer matrix method; analytical and numerical results show excellent agreement. This study can provide physical insight into the PIT coupling mechanisms and advance the applicability and versatility of PIT-based sensing platforms and other active devices. | SHENG-XUAN XIA XIANG ZHAI LING-LING WANG SHUANG-CHUN WEN | 2018 | Photonics Research2018，6，7： | 6 |
| 19 | Adapting rice anther culture to gene transformation and RNA interference显示文摘Anther culture offers a rapid method of generating homozygous lines for breeding pro- gram and genetic analysis.To produce homozygous transgenic lines of rice(Oryza sativa L.)in one step,we developed an efficient protocol of anther-callus-based transformation mediated by Agro- bacterium after optimizing several factors influencing efficient transformation,including callus induc- tion and Agrobacterium density for co-cultivation.Using this protocol,we obtained 145 independent green transformants from five cultivars of japonica rice by transformation with a binary vector pCXK1301 bearing the rice gene,Xa21 for resistance to bacterial blight,of which 140 were further confirmed by PCR and Southern hybridization analysis,including haploids(32.1%),diploids(62.1%) and mixoploids(7.5%).Fifteen diploids were found to be doubled haploids,which accounted for 10.7%of the total positive lines.Finally,by including 28 from colchicine induced or spontaneous dip- loidization of haploids later after transformation,a total of 43 doubled haploids(30.7%)of Xa21 transgenic lines were obtained.We also generated two RNAi transgenic haploids of the rice Os- MADS2 gene,a putative redundant gene of OsMADS4 based on their sequence similarity,to inves- tigate its possible roles in rice flower development by this method.Flowers from the two OsMADS2 RNAi transgenic haploids displayed obvious homeotic alternations,in which lodicules were trans- formed into palea/lemma-like tissues,whereas identities of other floral organs were maintained.The phenotypic alternations were proved to result from specific transcriptional suppression of OsMADS2 gene by the introduced RNAi transgene.The results confirmed that OsMADS2 is involved in lodicule development of rice flower and functionally redundant with OsMADS4 gene.Our results demonstrated that rice anther culture could be adapted to gene transformation and RNAi analysis in rice. | CHEN Caiyan 1,2* ,XIAO Han 1,2* ,ZHANG Wenli 1,2 ,WANG Aiju 1,2 ,XIA Zhihui 1 ,LI Xiaobing 1 , ZHAI Wenxue 1 ,CHENG Zhukuan 1 &ZHU Lihuang 1 1.State Key Laboratory of Plant Genomics and National Center for Plant Gene Research,Institute of Genetics and Develop- mental Biology,Chinese Academy of Sciences,Beijing 100101,China 2.Graduate School of the Chinese Academy of Sciences,Beijing 100039,China | 2006 | Science China(Life Sciences)2006，49，5： | 5 |
| 20 | Morphological, cytological and molecular analyses of a synthetic hexaploid derived from an interspecific hybrid between Gossypium hirsutum and Gossypium anomalum显示文摘Gossypium anomalum represents an inestimable source of genes that could potentially be transferred into the gene pool of cultivated cotton. To resolve interspecific hybrid sterility problems, we previously treated triploid hybrids derived from a cross between Gossypium hirsutum and G. anomalum with 0.15% colchicine and obtained a putative fertile hexaploid. In this study, we performed morphological, molecular and cytological analyses to assess the hybridity and doubled status of putative interspecific hybrid plants. Most of the morphological characteristics of the putative hexaploid plants were intermediate between G. hirsutum and G.anomalum. Analysis of mitotic metaphase plates revealed 78 chromosomes, confirming the doubled hybrid status of the hexaploid. Genome-wide molecular analysis with different genome-derived SSR markers revealed a high level of polymorphism(96.6%) between G. hirsutum and G. anomalum. The marker transferability rate from other species to G. anomalum was as high as 98.0%. The high percentage of polymorphic markers with additive banding profiles in the hexaploid indicates the hybridity of the hexaploid on a genome-wide level. A-genome-derived markers were more powerful for distinguishing the genomic differences between G. hirsutum and G. anomalum than D-genome-derived markers. This study demonstrates the hybridity and chromosomally doubled status of the(G. anomalum × G. hirsutum)2hexaploid using morphological, cytological and molecular marker methods. The informative SSR markers screened in the study will be useful marker resources for tracking the flow of G. anomalum genetic material among progenies that may be produced by future backcrosses to G. hirsutum. | Xia Zhang Caijiao Zhai Linchi He Qi Guo Xianggui Zhang Peng Xu Hongmei Su Yuanyong Gong Wanchao Ni Xinlian Shen | 2014 | The Crop Journal2014，2，5： | 5 |
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