|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | QTG-Seq Accelerates QTL Fine Mapping through QTL Partitioning and Whole-Genome Sequencing of Bulked Segregant Samples显示文摘Deciphering the genetic mechanisms underlying agronomic traits is of great importance for crop improvement. Most of these traits are controlled by multiple quantitative trait loci (QTLs), and identifying the underlying genes by conventional QTL fine-mapping is time-consuming and labor-intensive. Here, we devised a new method, named quantitative trait gene sequencing (QTG-seq), to accelerate QTL fine-mapping. QTGseq combines QTL partitioning to convert a quantitative trait into a near-qualitative trait, sequencing of bulked segregant pools from a large segregating population, and the use of a robust new algorithm for identifying candidate genes. Using QTG-seq, we fine-mapped a plant-height QTL in maize (Zea mays L.), qPH7, to a 300-kb genomic interval and verified that a gene encoding an NF-YC transcription factor was the functional gene. Functional analysis suggested that qPH7-encoding protein might influence plant height by interacting with a CO-like protein and an AP2 domain-containing protein. Selection footprint ana卜 ysis indicated that qPH7 was subject to strong selection during maize improvement. In summary, QTG-seq provides an efficient method for QTL fine-mapping in the era of “big data'. | Hongwei Zhang Xi Wang Qingchun Pan Pei Li Yunjun Liu Xiaoduo Lu Wanshun Zhong Minqi Li Linqian Han Juan Li Pingxi Wang Dongdong Li Yan Liu Qing Li Fang Yang Yuan-Ming Zhang Guoying Wang Lin Li | 2019 | Molecular Plant2019，12，3： | 13 |
| 2 | The Arabidopsis MutS homolog AtMSH5 is required for normal meiosis显示文摘MSH5,傻瓜相当或相同的事物脱氧核糖核酸失配修理蛋白质家庭的一个成员,被显示了在象老鼠,开始发育的酵母和 Caenorhabditis elegans 那样的多样的有机体为合适的同源染色体再结合被要求。在这篇论文,我们证明带通常认为的破坏 AtMSH5 基因的变异的 Arabidopsis 植物在 meiotic 期间展出缺点,生产不能生存的花粉粒和反常胚囊的一个比例,并且从而导致在富饶的减少。AtMSH5 表示被限制到 meiotic 花发芽,它在成熟分裂期间与一个可能的角色一致。男成熟分裂的 Cytological 分析从 diplotene 揭示了众多的 univalents 的存在到中期我,它在交叉频率与大减小被联系。在异种的剩余 chiasmata 的平均数字每性母细胞被归结为 2.54,它在野生型占约 25% 数量。这里,量的细胞遗传学的分析表明在 Atmsh5 异种的剩余 chiasmata 在 meiocytes 之中是随机分布式的,建议 AtMSH5 有在干扰敏感的交叉形成期间的一个必要角色。总起来说,证据显示 AtMSH5 通过在前期期间便于交叉形成支持相应再结合我在 Arabidopsis。 | Xiaoduo Lu Xiaolin Liu Lizhe An Wei Zhang Jian Sun Huijuan Pei Hongyan Meng Yunliu Fan Chunyi Zhang | 2008 | Cell Research2008，18，5： | 6 |
| 3 | Regulation of Leaf Angle by Auricle Development n Maize显示文摘 | Fanying Kong Tingting Zhang Jisheng Liu Siqi Heng Qingbiao Shi Haisen Zhang Zeli Wang Lei Ge Pinghua Li Xiaoduo Lu Gang Li | 2017 | Molecular Plant2017，10，3： | 5 |
| 4 | Gene-lndexed Mutations in Maize显示文摘 | Xiaoduo Lu Jisheng Liu Wen Ren Qun Yang Zhenguang Chai Rumei Chen Lei Wang Jun Zhao Zhihong Lang Haiyang Wang Yunliu Fan Jiuran Zhao Chunyi Zhang | 2018 | Molecular Plant2018，11，3： | 4 |
| 5 | Integration of high-throughput phenotyping,GWAS,and predictive models reveals the genetic architecture of plant height in maize显示文摘Plant height(PH)is an essential trait in maize(Zea mays)that is tightly associated with planting density,biomass,lodging resistance,and grain yield in the field.Dissecting the dynamics of maize plant architecture will be beneficial for ideotype-based maize breeding and prediction,as the genetic basis controlling PH in maize remains largely unknown.In this study,we developed an automated high-throughput phenotyping platform(HTP)to systematically and noninvasively quantify 77 image-based traits(i-traits)and 20 field traits(f-traits)for 228 maize inbred lines across all developmental stages.Time-resolved i-traits with novel digital phenotypes and complex correlations with agronomic traits were characterized to reveal the dynamics of maize growth.An i-trait-based genome-wide association study identified 4945 traitassociated SNPs,2603 genetic loci,and 1974 corresponding candidate genes.We found that rapid growth of maize plants occurs mainly at two developmental stages,stage 2(S2)to S3 and S5 to S6,accounting for the final PH indicators.By integrating the PH-association network with the transcriptome profiles of specific internodes,we revealed 13 hub genes that may play vital roles during rapid growth.The candidate genes and novel i-traits identified at multiple growth stages may be used as potential indicators for final PH in maize.One candidate gene,ZmVATE,was functionally validated and shown to regulate PH-related traits in maize using genetic mutation.Furthermore,machine learning was used to build predictive models for final PH based on i-traits,and their performancewas assessed across developmental stages.Moderate,strong,and very strong correlations between predictions and experimental datasets were achieved from the early S4(tenth-leaf)stage.Colletively,our study provides a valuable tool for dissecting the spatiotemporal formation of specific internodes and the genetic architecture of PH,as well as resources and predictive models that are useful for molecular design breeding and predicting maize varieties with ideal plant architectures. | Weixuan Wang Weijun Guo Liang Le Jia Yu Yue Wu Dongwei Li Yifan Wang Huan Wang Xiaoduo Lu Hong Qiao Xiaofeng Gu Jian Tian Chunyi Zhang Li Pu | 2023 | Molecular Plant2023，16，2： | 1 |
| 6 | An in vitro study to explore the role of prolylcarboxypeptidase in non-small cell lung cancer显示文摘Prolylcarboxypeptidase(PRCP)belongs to the S28 family of proteases,which is also a dipeptidyl peptidase.In this study,we demonstrate the expression pattern of PRCP in Non-small cell lung cancer(NSCLC).We found that the repression of PRCP expression by small interfering RNA successfully inhibited cell proliferation,migration,and invasion.Further,we explored the involvement of PRCP in the regulation of epithelial-mesenchymal transition(EMT).The epithelial marker E-cadherin was significantly increased,meanwhile mesenchymal markers MUC1,vimentin,and SNAIL were markedly decreased in PRCP knockdown cells.Moreover,the downregulation of PRCP in the NSCLC cells induced the expression of apoptosis-related proteins in vitro.We performed RT-PCR in 30 pairs of clinical NSCLC tissues and adjacent non-cancerous tissues,which revealed significantly higher PRCP expression levels in cancer tissues than in adjacent non-cancerous tissues.Collectively the results from our study suggest a possible cancer promotion role of PRCP in NSCLC. | Binbin QIAN Xiaoduo LIU Xiaolin GU Lu YANG Dake CHEN | 2020 | BIOCELL2020，44，1： | 0 |
| 7 | VAMP726 from maize and Arabidopsis confers pollen resistance to heat and UV radiation by influencing lignin content of sporopollenin显示文摘Sporopollenin in the pollen cell wall protects male gametophytes from stresses.Phenylpropanoid derivatives,including guaiacyl(G)lignin units,are known to be structural components of sporopollenin,but the exact composition of sporopollenin remains to be fully resolved.We analyzed the phenylpropanoid derivatives in sporopollenin from maize and Arabidopsis by thioacidolysis coupled with nuclear magnetic resonance(NMR)and gas chromatography–mass spectrometry(GC–MS).The NMR and GC–MS results confirmed the presence of p-hydroxyphenyl(H),G,and syringyl(S)lignin units in sporopollenin from maize and Arabidopsis.Strikingly,H units account for the majority of lignin monomers in sporopollenin from these species.We next performed a genome-wide association study to explore the genetic basis of maize sporopollenin composition and identified a vesicle-associated membrane protein(ZmVAMP726)that is strongly associated with lignin monomer composition of maize sporopollenin.Genetic manipulation of VAMP726 affected not only lignin monomer composition in sporopollenin but also pollen resistance to heat and UV radiation in maize and Arabidopsis,indicating that VAMP726 is functionally conserved in monocot and dicot plants.Our work provides new insight into the lignin monomers that serve as structural components of sporopollenin and characterizes VAMP726,which affects sporopollenin composition and stress resistance in pollen. | Wenqi Yang Dongdong Yao Haiyang Duan Junli Zhang Yaling Cai Chen Lan Bing Zhao Yong Mei Yan Zheng Erbing Yang Xiaoduo Lu Xuehai Zhang Jihua Tang Ke Yu Xuebin Zhang | 2023 | Plant Communications2023，4，6： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员