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| 1 | Erianin,a novel dibenzyl compound in Dendrobium extract,inhibits lung cancer cell growth and migration via calcium/calmodulin-dependent ferroptosis显示文摘Ferroptosis,a novel form of programmed cell death,is characterized by iron-dependent lipid peroxidation and has been shown to be involved in multiple diseases,including cancer.Stimulating ferroptosis in cancer cells may be a potential strategy for cancer therapy.Therefore,ferroptosis-inducing drugs are attracting more attention for cancer treatment.Here,we showed that erianin,a natural product isolated from Dendrobium chrysotoxum Lindl,exerted its anticancer activity by inducing cell death and inhibiting cell migration in lung cancer cells.Subsequently,we demonstrated for the first time that erianin induced ferroptotic cell death in lung cancer cells,which was accompanied by ROS accumulation,lipid peroxidation,and GSH depletion.The ferroptosis inhibitors Fer-1 and Lip-1 but not Z-VAD-FMK,CQ,or necrostatin-1 rescued erianin-induced cell death,indicating that ferroptosis contributed to erianin-induced cell death.Furthermore,we demonstrated that Ca^(2+)/CaM signaling was a critical mediator of erianin-induced ferroptosis and that blockade of this signaling significantly rescued cell death induced by erianin treatment by suppressing ferroptosis.Taken together,our data suggest that the natural product erianin exerts its anticancer effects by inducing Ca^(2+)/CaMdependent ferroptosis and inhibiting cell migration,and erianin will hopefully serve as a prospective compound for lung cancer treatment. | Peng Chen Qibiao Wu Jiao Feng Lili Yan Yitian Sun Shuiping Liu Yu Xiang Mingming Zhang Ting Pan Xiaying Chen Ting Duan Lijuan Zhai Bingtao Zhai Wengang Wang Ruonan Zhang Bi Chen Xuemeng Han Yicong Li Liuxi Chen Ying Liu Xingxing Huang Ting Jin Wenzheng Zhang Hong Luo Xiaohui Chen Yongqiang Li Qiujie Li Guohua Li Qin Zhang Lvjia Zhuo Zuyi Yang Huifen Tang Tian Xie Xiaoping Ouyang Xinbing Sui | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 42 |
| 2 | Preparation, characterization, pharmacokinetics and anticancer effects of PEGylated β-elemene liposomes显示文摘Objective:This study aimed to develop a new polyethylene glycol(PEG)ylatedβ-elemene liposome(PEG-Lipo-β-E)and evaluate its characterization,pharmacokinetics,antitumor effects and safety in vitro and in vivo.Methods:The liposomes were prepared by ethanol injection and high-pressure micro-jet homogenization.Characterization of the liposomes was conducted,and drug content,entrapment efficiency(EE),in vitro release and stability were studied by ultra-fast liquid chromatography(UFLC)and a liquid surface method.Blood was drawn from rats to establish the pharmacokinetic parameters.The anticancer effect was evaluated in a KU-19-19 bladder cancer xenograft model.Histological analyses were performed to evaluate safety.Results:The PEG-Lipo-β-E showed good stability and was characterized as 83.31±0.181 nm in size,0.279±0.004 in polydispersity index(PDI),-21.4±1.06 mV in zeta potential,6.65±0.02 in pH,5.024±0.107 mg/mL inβ-elemene(β-E)content,and 95.53±1.712%in average EE.The Fourier transform infrared spectroscopy(FTIR)and differential scanning calorimetry(DSC)indicated the formation of PEG-Lipo-β-E.Compared to elemene injection,PEG-Lipo-β-E demonstrated a 1.75-fold decrease in clearance,a 1.62-fold increase in half-life,and a 1.76-fold increase in area under the concentration-time curves(AUCs)from 0 hour to 1.5 hours(P<0.05).PEG-Lipo-β-E also showed an enhanced anticancer effect in vivo.Histological analyses showed that there was no evidence of toxicity to the heart,kidney,liver,lung or spleen.Conclusions:The present study demonstrates PEG-Lipo-β-E as a new formulation with ease of preparation,high EE,good stability,improved bioavailability and antitumor effects. | Bingtao Zhai Qibiao Wu Wengang Wang Mingming Zhang Xuemeng Han Qiujie Li Peng Chen Xiaying Chen Xingxing Huang Guohua Li Qin Zhang Ruonan Zhang Yu Xiang Shuiping Liu Ting Duan Jianshu Lou Tian Xie Xinbing Sui | 2020 | Cancer Biology & Medicine2020，17，1： | 6 |
| 3 | An ATF24 peptide-functionalized β-elemene-nanostructured lipid carrier combined with cisplatin for bladder cancer treatment显示文摘Objective:In this study,we aimed to develop an amino-terminal fragment(ATF)peptide-targeted liposome carryingβ-elemene(ATF24-PEG-Lipo-β-E)for targeted delivery into urokinase plasminogen activator receptor-overexpressing bladder cancer cells combined with cisplatin(DDP)for bladder cancer treatment.Methods:The liposomes were prepared by ethanol injection and high-pressure microjet homogenization.The liposomes were characterized,and the drug content,entrapment efficiency,andin vitro release were studied.The targeting efficiency was investigated using confocal microscopy,ultra-fast liquid chromatography,and an orthotopic bladder cancer model.The effects of ATF24-PEG-Lipo-β-E combined with DDP on cell viability and proliferation were evaluated by a Cell Counting Kit-8(CCK-8)assay,a colony formation assay,and cell apoptosis and cell cycle analyses.The anticancer effects were evaluated in a KU-19-19 bladder cancer xenograft model.Results:ATF24-PEG-Lipo-β-E had small and uniform sizes(~79 nm),high drug loading capacity(~5.24 mg/mL),high entrapment efficiency(98.37±0.95%),and exhibited sustained drug release behavior.ATF24-PEG-Lipo-β-E had better targeting efficiency and higher cytotoxicity than polyethylene glycol(PEG)ylatedβ-elemene liposomes(PEG-Lipo-β-E).DDP,combined with ATF24-PEG-Lipo-β-E,exerted a synergistic effect on cellular apoptosis and cell arrest at the G2/M phase,and these effects were dependent on the caspase-dependent pathway and Cdc25C/Cdc2/cyclin B1 pathways.Furthermore,thein vivo antitumor activity showed that the targeted liposomes effectively inhibited the growth of tumors,using the combined strategy.Conclusions:The present study provided an effective strategy for the targeted delivery ofβ-elemene(β-E)to bladder cancer,and a combined strategy for bladder cancer treatment. | Bingtao Zhai Peng Chen Wengang Wang Shuiping Liu Jiao Feng Ting Duan Yu Xiang Ruonan Zhang Mingming Zhang Xuemeng Han Xiaying Chen Qiujie Li Guohua Li Ying Liu Xingxing Huang Wenzheng Zhang Ting Pan Lili Yan Ting Jin Tian Xie Xinbing Sui | 2020 | Cancer Biology & Medicine2020，17，3： | 5 |
| 4 | A method to model the effect of pre-existing cracks on P-wave velocity in rocks显示文摘Crack closure is one of the reasons inducing changes of P-wave velocity of rocks under compression.In this context,a method is proposed to investigate the relationships among P-wave velocity,pre-existing cracks,and confining pressure based on the discrete element method(DEM).Pre-existing open cracks inside the rocks are generated by the initial gap of the flat-joint model.The validity of the method is evaluated by comparing the P-wave velocity tested on a sandstone specimen with numerical result.As the crack size is determined by the diameter of particles,the effects of three factors,i.e.number,aspect ratio,and orientation of cracks on the P-wave velocity are discussed.The results show that P-wave velocity is controlled by the(i.e.number) of open micro-cracks,while the closure pressure is determined by the aspect ratio of crack.The reason accounting for the anisotropy of P-wave velocity is the difference in crack number in measurement paths.Both of the number and aspect ratio of cracks can affect the responses of P-wave velocity to the applied confining pressure.Under confining pressure,the number of open cracks inside rocks will dominate the lowest P-wave velocity,and the P-wave velocity of the rock containing narrower cracks is more sensitive to the confining pressure.In this sense,crack density is difficult to be back-calculated merely by P-wave velocity.The proposed method offers a means to analyze the effect of pre-existing cracks on P-wave velocity. | Haimeng Shen Xiaying Li Qi Li Haibin Wang | 2020 | Journal of Rock Mechanics and Geotechnical Engineering2020，12，3： | 5 |
| 5 | 氮沉降模拟实验亟待研究大气氨浓度增加的影响显示文摘大气活性氮污染及其过量沉降是全球变化重要议题。为研究氮沉降对自然生态系统结构和功能的影响,基于喷淋加氮的控制实验在草地或林下广泛开展。该实验体系假定氮沉降是通过增加土壤氮的有效性从而影响整个生态系统,没有考虑植被与大气活性氮的相互作用,也未能反映氮沉降以干沉降为主的自然过程。考虑到大气氨是氮沉降的主要前体物,且全球氨气浓度呈现上升趋势,未来的氮沉降模拟实验亟待研究氨气干沉降的重要影响,以期更加完整的刻画大气氮沉降过程及其生态环境效应。 | PAN Yuepeng TIAN Shili WU Dianming XU Wen ZHU Xiaying LIU Chunyan LI Dejun FANG Yunting DUAN Lei LIU Xuejun WANG Yuesi | 2020 | Atmospheric and Oceanic Science Letters2020，13，3： | 5 |
| 6 | PCDH17 increases the sensitivity of colorectal cancer to 5-fluorouracil treatment by inducing apoptosis and autophagic cell death显示文摘5-Fluorouracil(5-FU)is known as a first-line chemotherapeutic agent against colorectal cancer(CRC),but drug resistance occurs frequently and significantly limits its clinical success.Our previous study showed that the protocadherin 17(PCDH17)gene was frequently methylated and functioned as a tumor suppressor in CRC.However,the relationship between PCDH17 and 5-FU resistance in CRC remains unclear.Here,we revealed that PCDH17 was more highly expressed in 5-FU-sensitive CRC tissues than in 5-FU-resistant CRC tissues,and high expression of PCDH17 was correlated with high BECN1 expression.Moreover,this expression profile contributed to superior prognosis and increased survival in CRC patients.Restoring PCDH17 expression augmented the 5-FU sensitivity of CRC in vitro and in vivo by promoting apoptosis and autophagic cell death.Furthermore,autophagy played a dominant role in PCDH17-induced cell death,as an autophagy inhibitor blocked cell death to a greater extent than the pancaspase inhibitor Z-VAD-FMK.PCDH17 inhibition by siRNA decreased the autophagy response and 5-FU sensitivity.Mechanistically,we showed that c-Jun NH2-terminal kinase(JNK)activation was a key determinant in PCDH17-induced autophagy.The compound SP600125,an inhibitor of JNK,suppressed autophagy and 5-FU-induced cell death in PCDH17-reexpressing CRC cells.Taken together,our findings suggest for the first time that PCDH17 increases the sensitivity of CRC to 5-FU treatment by inducing apoptosis and JNK-dependent autophagic cell death.PCDH17 may be a potential prognostic marker for predicting 5-FU sensitivity in CRC patients. | Shuiping Liu Haoming Lin Da Wang Qiang Li Hong Luo Guoxiong Li Xiaohui Chen Yongqiang Li Peng Chen Bingtao Zhai Wengang Wang Ruonan Zhang Bi Chen Mingming Zhang Xuemeng Han Qiujie Li Liuxi Chen Ying Liu Xiaying Chen Guohua Li Yu Xiang Ting Duan Jiao Feng Jianshu Lou Xingxing Huang Qin Zhang Ting Pan Lili Yan Ting Jin Wenzheng Zhang Lvjia Zhuo Yitian Sun Tian Xie Xinbing Sui | 2019 | Signal Transduction and Targeted Therapy2019，4，1： | 4 |
| 7 | Copy number and zygosity determination of transgenic rapeseed by droplet digital PCR显示文摘Establishing an accurate and rapid method for copy number and zygosity determination can accelerate genetic engineering research process. In this study, droplet digital PCR (ddPCR), an emerging DNA absolute quantification technology, was used to identify single-copy homozygous transgenic lines from a batch of T0 transgenic rapeseed harboring 11 exogenous elements. Copy number of exogenous gene was evaluated in T0 generation based on calculated ratio between transgene and reference CruA gene, single-copy transformants were selected for selfing followed by subsequent zygosity analysis. Single-copy homozygous transgenic plants were successfully screened out in T1 generation by ddPCR.Segregation analysis with T2 seedlings verified that identification results of ddPCR were accurate and reliable. This study provides a novel rapid and accurate method for copy number and zygosity determination in transgenic rapeseed which overcomes disadvantages of traditional Southern analysis and recently developed real-time quantitative method. | Yuhua Wu Jun Li Xiaying Li Jingang Liang Yunjing Li Xinhua Zeng Gang Wu | 2017 | Oil Crop Science2017，2，2： | 4 |
| 8 | 塔里木盆地碳酸盐岩储层酸性气体注入的数值模拟及可行性评价显示文摘塔里木盆地油气藏开采过程中产生了大量的酸性气体,其中包括CO2和H2S。安全处置酸性气体需要适合的管理计划,普遍策略是将其注入深层地层——这样可减少温室气体排放,避免脱硫产生的成本。注酸气的可行性评价需要对其潜在的物理和地球化学影响进行详细的调查。通过塔里木盆地碳酸盐岩地层的矿物组成和水化学特征,对酸性气体与矿物基质和地层水的反应运移进行了模拟研究,确定了酸性气体与矿物基质和地层水的物理和地球化学相互作用。以19200 m^3/d(20℃,1.013×10^5 Pa)的恒定速率注入酸性气体(含59%的CO2和41%的H2S)25年,使用TOUGHREACT代码的TMVR_EOSG模块进行模拟,模拟时间为1万年。结果表明,最小含水饱和度远大于残余含水饱和度,压力累积值低于允许压力增加值。 | Xiaoyan Zhang Qi Li Liange Zheng Xiaying Li and Liang Xu 陆倩 | 2020 | 油气田地面工程2020，39，6： | 2 |
| 9 | Hydromechanical behaviors of andesite under different stress states during fluid injection显示文摘Water reinjection into the formation is an indispensable operation in many energy engineering practices.This operation involves a complex hydromechanical(HM)coupling process and sometimes even causes unpredictable disasters,such as induced seismicity.It is acknowledged that the relative magnitude and direction of the principal stresses significantly influence the HM behaviors of rocks during injection.However,due to the limitations of current testing techniques,it is still difficult to comprehensively conduct laboratory injection tests under various stress conditions,such as in triaxial extension stress states.To this end,a numerical study of HM changes in rocks during injection under different stress states is conducted.In this model,the saturated rock is first loaded to the target stress state under drainage conditions,and then the stress state is maintained and water is injected from the top to simulate the formation injection operation.Particular attention is given to the difference in HM changes under triaxial compression and extension stresses.This includes the differences in the pore pressure propagation,mean effective stress,volumetric strain,and stress-induced permeability.The numerical results demonstrate that the differential stress will significantly affect the HM behaviors of rocks,but the degree of influence is different under the two triaxial stress states.The HM changes caused by the triaxial compression stress states are generally greater than those of extension,but the differences decrease with increasing differential stress,indicating that the increase in the differential stress will weaken the impact of the stress state on the HM response.In addition,the shear failure potential of fracture planes with various inclination angles is analyzed and summarized under different stress states.It is recommended that engineers could design suitable injection schemes according to different tectonic stress fields versus fault occurrence to reduce the risk of injection-induced seismicity. | Miao He Qi Li Xiaying Li Liang Xu Michael Kühn | 2021 | Journal of Rock Mechanics and Geotechnical Engineering2021，13，4： | 1 |
| 10 | Black titanium dioxide nanomaterials for photocatalytic removal of pollutants:A review显示文摘Semiconductor photocatalysis is one of the most widely used environment-friendly technologies for removing various contaminants.As a well-developed photocatalyst,titanium dioxide(TiO_(2))still has limits in its wide bandgap and rapid recombination rate of photogenerated charge carriers.Recently,black TiO_(2)appears as a strong candidate in the improvement of sunlight harvesting,because of its excellent absorption capacity and utilization of solar radiation.Despite extensive applications in both environmental and energy fields,the use of black TiO_(2)as a photocatalyst in pollutant removal is ambiguous.The primary objective of the review is to comprehensively evaluate the applications of black TiO_(2)in photocatalytic removal of contaminants,including conventional organic contaminants,emerging contaminants,microbes,and heavy metals.The basic properties,photocatalytic mechanism,and synthesis of black TiO_(2)have been summarized and analyzed.Moreover,the stability and recoverability of black TiO_(2)have also been discussed.Finally,the perspectives of the application of black TiO_(2)in pollutant removal have been further discussed. | Ying Liang Guohe Huang Xiaying Xin Yao Yao Yongping Li Jianan Yin Xiang Li Yuwei Wu Sichen Gao | 2022 | Journal of Materials Science & Technology2022，，17： | 1 |
| 11 | An effective method for laboratory acoustic emission detection and location using template matching显示文摘In this paper, a template matching and location method, which has been rapidly adopted in microseismic research in recent years, is applied to laboratory acoustic emission(AE) monitoring. First, we used traditional methods to detect P-wave first motions and locate AE hypocenters in three dimensions. In addition, we selected events located with sufficient accuracy(normally corresponding AE events of relatively larger energy, showing clear P-wave first motion and a higher signal-to-noise ratio in most channels) as template events. Then, the template events were used to scan and match other poorly located events in triggered event records or weak events in continuous records. Through crosscorrelation of the multi-channel waveforms between the template and the event to be detected, the weak signal was detected and located using a grid-searching algorithm(with the grid centered at the template hypocenter). In order to examine the performance of the approach, we calibrated the proposed method using experimental data of different rocks and different types of experiments. The results show that the proposed method can significantly improve the detection capability and location accuracy, and can be applied to various laboratory and in situ experiments, which use multi-channel AE monitoring with waveforms recorded in either triggering or continuous mode. | Xinglin Lei Tomohiro Ohuchi Manami Kitamura Xiaying Li Qi Li | 2022 | Journal of Rock Mechanics and Geotechnical Engineering2022，14，5： | 1 |
| 12 | The effect of p21 antisense oligodeoxynucleotides on the radiosensitivity of nasopharyngeal carcinoma cells with normal p53 function 显示文摘 | Liu X F XiaY F Li M Z | 2006 | Cell Biol Int2006，30，3： | 1 |
| 13 | Antisense vimentin cDNA combined with chon- droitinase ABC reduces glial scar and cystic cavity formation following spinal cord injury in rats显示文摘 | XiaY Zhao T Li J | 2008 | Biochem Biophys Res Commun2008，377，2： | 1 |
| 14 | A Precursory Signal of June-July Precipitation over the Yangtze River Basin: December-January Tropospheric Temperature over the Tibetan Plateau显示文摘The prediction of summer precipitation over the Yangtze River basin(YRB)has long been challenging,especially during June-July(JJ),when the mei-yu generally occurs.This study explores the potential signal for the YRB precipitation in JJ and reveals that the Tibetan Plateau tropospheric temperature(TPTT)in the middle and upper levels during the preceding December-January(DJ)is significantly correlated with JJ YRB precipitation.The close connection between the DJ TPTT anomaly with JJ YRB precipitation may be due to the joint modulation of the DJ ENSO and spring TP soil temperatures.The lagged response to an anomalously cold TPTT during the preceding DJ is a TPTT that is still anomalously cold during the following JJ.The lower TPTT can lead to an anomalous anticyclone to the east of Lake Baikal,an anomalous cyclone at the middle latitudes of East Asia,and an anomalous anticyclone over the western North Pacific.Meanwhile,the East Asian westerly jet shifts southward in response to the meridional thermal gradient caused by the colder troposphere extending from the TP to the east of Lake Baikal.The above-mentioned circulation anomalies constitute the positive anomaly of the East Asia-Pacific pattern,known to be conducive to more precipitation over the YRB.Since the DJ TPTT contains both the land(TP soil temperature)and ocean(ENSO)signals,it has a closer relationship with the JJ precipitation over the YRB than the DJ ENSO alone.Therefore,the preceding DJ TPTT can be considered an alternative predictor of the JJ YRB precipitation. | Xiaying ZHU Mingzhu YANG Ge LIU Yanju LIU Weijing LI Sulan NAN Linhai SUN | 2023 | Advances in Atmospheric Sciences2023，40，11： | 1 |
| 15 | On Water: Free Catalysis Approach for the Synthesis of Nitrones from Hydroxamic Acids and Dimethy/ Diethyl Acetylenedicarboxylate显示文摘Benzohydroxamic 酸面对 10 mol% NaOAc 与 dimethyl acetylenedicarboxylate 反应为 2 h 在房间温度在水里在 good-to-excellent 产量产生(E)-N-(1,4-dimethoxy-1,4-dioxobutan-2-ylidene)-1-phenylcarboxamide 氧化物,它供应一个简单、有效、环境地友好的方法综合大量 nitrones。这策略的好处不仅遵循绿 chemisty 的 requirment,而且拥有原子经济。 | Jian Li Yongqin He Xiaoyu Ren Xiaokang Shi Sizhuo Yang Xiai Gao Guosheng Huang | 2013 | Chinese Journal of Chemistry2013，31，8： | 1 |
| 16 | Influence of bedding structure on stress-induced elastic wave anisotropy in tight sandstones显示文摘To understand the evolution of stress-induced elastic wave anisotropy,three triaxial experiments were performed on sandstone specimens with bedding orientations parallel,perpendicular,and oblique to the maximum principal stress.P-wave velocities along 64 different directions on each specimen were monitored frequently to understand the anisotropy change at various stress levels by fitting Thomsen’s anisotropy equation.The results show that the elastic wave anisotropy is very sensitive to mechanical loading.Under hydrostatic loading,the magnitude of anisotropy is reduced in all three specimens.However,under deviatoric stress loading,the evolution of anisotropic characteristics(magnitude and orientation of the symmetry axis)is bedding orientation dependent.Anisotropy reversal occurs in specimens with bedding normal/oblique to the maximum principal stress.P-wave anisotropyε0 is linearly related to volumetric strain Sv and dilatancy,indicating that stress-induced redistribution of microcracks has a significant effect on P-wave velocity anisotropy.The closure of initial cracks and pores aligned in the bedding direction contributes to the decrease of the anisotropy.However,opening of new cracks,aligned in the maximum principal direction,accounts for the increase of the anisotropy.The experimental results provide some insights into the microstructural behavior under loading and provide an experimental basis for seismic data interpretation and parameter selection in engineering applications. | Xiaying Li Xinglin Lei Qi Li Dianguo Chen | 2021 | Journal of Rock Mechanics and Geotechnical Engineering2021，13，1： | 1 |
| 17 | Palladium-catalyzed cascade synthesis of spirocyclic oxindoles via regioselective C2-H arylation and C8-H alkylation of naphthalene ring显示文摘A simultaneous C2-H arylation and C8-H alkylation of naphthalene ring has been achieved by palladiumcatalyzed cascade reaction of N-(2-halophenyl)-2-(naphthalen-1-yl)acrylamides with aryl iodides,which provides an efficient method for synthesizing various aryl-substituted spirocyclic oxindoles.The protocol enables three C-C bonds formation via an intramolecular Heck reaction and sequentially regioselective C-H bond activation. | Xiai Luo Wenguang Li Haiyan Lu Guobo Deng Yuan Yang Chunming Yang Yun Liang | 2021 | Chinese Chemical Letters2021，32，2： | 0 |
| 18 | Comparison of Five Endogenous Reference Genes for Specific PCR Detection and Quantification of Rice显示文摘Endogenous reference genes (ERGs) provide vital information regarding genetically modified organisms (GMOs). The successful detection of ERGs can identity GMOs and the source of genes, verify stability and reliability of the detection system, and calculate the level of genetically modified (GM) ingredients in mixtures. The reported ERGs in rice include sucrose-phosphate synthase (SPS), phospholipase D (PLD), RBE4 and rice root-specific GOS9 genes. Based on the characteristics of ERGs, a new ERG gene, phosphoenolpyruvate carboxylase (PEPC), was selected, and further compared with the four existing genes. A total of 18 rice varieties and 29 non-rice crops were used to verify the interspecies specificity, intraspecies consistency, sensitivity, stability and reliability of these five ERGs using qualitative and quantitative PCR. Qualitative detection indicated that SPS and PEPC displayed sufficient specificity, and the detection sensitivity was 0.05% and 0.005%, respectively. Although the specificity of both RBE4 and GOS9 were adequate, the amplicons were small and easily confused with primer dimers. Non-specific amplification of the PLD gene was present in maize and potato. Real-time quantitative PCR detection indicated that PLD, SPS and PEPC displayed good specificity, with R2 of the standard curve greater than 0.98, while the amplification efficiency ranged between 90% and 110%. Both the detection sensitivities of PLD and PEPC were five copies and that of SPS was ten copies. RBE4 showed typical amplification in maize, beet and Arabidopsis, while GOS9 was found in maize, tobacco and oats. PEPC exhibited excellent detection sensitivity and species specificity, which made it a potentially useful application in GM-rice supervision and administration. Additionally, SPS and PLD are also suitable for GM-rice detection. This study effectively established a foundation for GMO detection, which not only provides vital technical support for GMO identification, but also is of great significance for enhancing the comparability of detection results, and the standardization of ERG testing in GM-rice. | ZHANG Xiujie JIN Wujun XU Wentao LI Xiaying SHANG Ying LI Sha OUYANG Hongsheng | 2019 | Rice science2019，26，4： | 0 |
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