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| 1 | Effect of Zishenshengxue capsule on myelosuppression in mice induced by cyclophosphamide显示文摘OBJECTIVE:To explore the inhibitory effect of Zishenshengxue capsule(ZSC) on myelosuppression in mice induced by cyclophosphamide.METHODS:Kunming mice were randomly assigned into a control group,myelosuppression group,or groups for mice with myelosuppression receiving high dose ZSC,middle dose ZSC,low dose ZSC or Yixuesheng.Myelosuppression was induced by peritoneal injection of cyclophosphamide.ZSC and cyclophosphamide were administered simultaneously.The numbers of peripheral blood cells and bone marrow karyocytes were counted.Cell proliferation activity and colony formation of granulocyte-monocyte series hemopoietic progenitor cells(C-GMs) and cell cycle were detected.RESULTS:The numbers of white blood cells in the middle and high dose ZSC groups were significantly increased at the 12th and 13th day(P<0.01) and bone marrow karyocytes and cell proliferation activity increased in the high dose ZSC group(P<0.01) compared with the myelosuppression group.C-GMs of middle and high dose ZSC groups significantly increased(P<0.01).The percentage of G 1 phase in the high dose ZSC group decreased(P< 0.01) and the percentage of S and G 2 phase increased(P<0.01).CONCLUSION:ZSC increased the numbers of peripheral white blood cells,bone marrow karyocytes and C-GMs.ZSC also increased cell proliferation activity and removed the G 1 phase block.Thus,ZSC could reduce myelosuppression in mice induced by cyclophosphamide. | Wei Li Ying Zhao Xinna Li | 2013 | Journal of Traditional Chinese Medicine2013，33，2： | 3 |
| 2 | Identification of Nonstructural Protein 8 as the N-Terminus of the RNA-Dependent RNA Polymerase of Porcine Reproductive and Respiratory Syndrome Virus显示文摘Porcine reproductive and respiratory syndrome virus (PRRSV) is a member within the family Arteriviridae of the order Nidovirales. Replication of this positive-stranded RNA virus within the host cell involves expression of viral replicase proteins encoded by two ORFs, namely ORF1 a and ORF1 b. In particular, translation of ORF1 b depends on a-1-ribosomal frameshift strategy. Thus, nonstructural protein 9 (nsp9), the first protein within ORF1 b that specifies the function of the viral RNA-dependent RNA polymerase, is expressed as the C-terminal extension of nsp8, a small nsp that is encoded by ORF1 a. However, it has remained unclear whether the mature form of nsp9 in virus-infected cells still retains nsp8,addressing which is clearly critical to understand the biological function of nsp9. By taking advantage of specific antibodies to both nsp8 and nsp9, we report the following findings. (1) In infected cells, PRRSV nsp9 was identified as a major product with a size between 72 and 95 k Da (72–95 KDa form), which exhibited the similar mobility on the gel to the in vitro expressed nsp8–9 ORF1 b, but not the ORF1 b-coded portion (nsp9 ORF1 b). (2) The antibodies to nsp8, but not to nsp7 or nsp10, could detect a major product that had the similar mobility to the 72–95 KDa form of nsp9. Moreover, nsp9 could be co-immunoprecipitated by antibodies to nsp8, and vice versa. (3) Neither nsp4 nor nsp2 PLP2 was able to cleave nsp8–nsp9 in vitro. Together, our studies provide experimental evidence to suggest that nsp8 is an N-terminal extension of nsp9.Our findings here paves way for further charactering the biological function of PRRSV nsp9. | Yuanyuan Liu Yunhao Hu Yue Chai Liping Liu Jiangwei Song Shaochuan Zhou Jia Su Lei Zhou Xinna Ge Xin Guo Jun Han Hanchun Yang | 2018 | Virologica Sinica2018，33，5： | 3 |
| 3 | Nsp2 and GP5-M of Porcine Reproductive and Respiratory Syndrome Virus Contribute to Targets for Neutralizing Antibodies显示文摘Porcine reproductive and respiratory syndrome virus(PRRSV)is characterized by its genetic variation and limited cross protection among heterologous strains.Even though several viral structural proteins have been regarded as inducers of neutralizing antibodies(NAs)against PRRSV,the mechanism underlying limited cross-neutralization among heterologous strains is still controversial.In the present study,examinations of NA cross reaction between a highly pathogenic PRRSV(HP-PRRSV)strain,JXwn06,and a low pathogenic PRRSV(LP-PRRSV)strain,HB-1/3.9,were conducted with viral neutralization assays in MARC-145 cells.None of the JXwn06-hyperimmuned pigs’sera could neutralize HB-1/3.9 in vitro and vice versa.To address the genetic variation between these two viruses that are associated with limited crossneutralization,chimeric viruses with coding regions swapped between these two strains were constructed.Viral neutralization assays indicated that variations in nonstructural protein 2(nsp2)and structural proteins together contribute to weak cross-neutralization activity between JXwn06 and HB-1/3.9.Furthermore,we substituted the nsp2-,glycoprotein2(GP2)-,GP3-,and GP4-coding regions together,or nsp2-,GP5-,and membrane(M)protein-coding regions simultaneously between these two viruses to construct chimeric viruses to test cross-neutralization reactivity with hyperimmunized sera induced by their parental viruses.The results indicated that the swapped nsp2 and GP5-M viruses increased the neutralization reactivity with the donor strain antisera in MARC-145 cells.Taken together,these results show that variations in nsp2 and GP5-M correlate with the limited neutralization reactivity between the heterologous strains HP-PRRSV JXwn06 and LP-PRRSV HB-1/3.9. | Jia Su Lei Zhou Bicheng He Xinhui Zhang Xinna Ge Jun Han Xin Guo Hanchun Yang | 2019 | Virologica Sinica2019，34，6： | 3 |
| 4 | Changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2显示文摘 | Kaichuang Shi Huanrong Li Xin Guo Xinna Ge Hong Jia Shijun Zheng Hanchun Yang | 2007 | Veterinary Microbiology2007，，3： | 2 |
| 5 | Psoralen inhibits hepatitis B viral replication by down-regulating the host transcriptional machinery of viral promoters显示文摘The hepatitis B virus(HBV) is a global public health challenge due to its highly contagious nature. It is estimated that almost 300 million people live with chronic HBV infection annually. Although nucleoside analogs markedly reduce the risk of liver disease progression, the analogs do not fully eradicate the virus. As such, new treatment options and drugs are urgently needed. Psoralen is a nourishing monomer of Chinese herb and is known to inhibit virus replication and inactivate viruses. In this study, we evaluated the potential of psoralen as an anti-HBV agent.Quantitative PCR and Southern blot analysis revealed that psoralen inhibited HBV replication in Hep G2.2.15 cells in a concentration-dependent manner. Moreover, psoralen was also active against the 3TC/ETV-dual-resistant HBV mutant. Further investigations revealed that psoralen suppressed both HBV RNA transcription and core protein expression. The transcription factor FOXO1, a known target for PGC1α co-activation, binds to HBV precore/core promoter enhancer II region and activates HBV RNA transcription. Co-immunoprecipitation showed that psoralen suppressed the expression of FOXO1, thereby decreasing the binding of FOXO1 co-activator PGC1αto the HBV promoter. Overall, our results demonstrate that psoralen suppresses HBV RNA transcription by downregulating the expression of FOXO1 resulting in a reduction of HBV replication. | Xinna Ma Heng Li Ying Gong Feifei Liu Xiankun Tong Fenghua Zhu Xiaoqian Yang Li Yang Jianping Zuo | 2022 | Virologica Sinica2022，37，2： | 2 |
| 6 | Alteration of encephalomyocarditis virus pathogenicity due to a mutation at position 100 of VP1显示文摘Encephalomyocarditis virus (EMCV) infection leads to many diseases including encephalitis,myocarditis and diabetes in its natural host,the mouse.In this study,we generated four cDNA clones with a point mutation at position 100 of VP1.The amino acids isoleucine,alanine,serine and proline were substituted with threonine in the four different clones of EMCV strain BJC3 by site-specific mutagenesis,and viable viruses were rescued.Although all mutants and wild-type viruses display different plaque morphologies,they replicate comparably in BHK-21 cells.The pathogenicity of the mutated viruses was systematically analyzed to investigate the importance of this amino acid in the viral pathogenicity and disease phenotype of EMCV infection in mice.The results showed that the isoleucine(T1100I) and proline-mutated viruses (T1100P) exhibited a reduced mortality,lower cerebral virus loads and alleviated brain damage while the viruses with serine (T1100S) and alanine (T1100A) substitutions displayed similar properties as the wild-type virus.These findings indicate that the amino acid at position 100 of VP1 is important for EMCV in vivo infection,and its mutation alters the pathogenicity of viral infection in mice. | ZHU Shu GE XinNa GONG XiaoWen GUO Xin CHEN YanHong YANG HanChun | 2011 | Science China(Life Sciences)2011，54，6： | 2 |
| 7 | ABCC8 and ABCC9: ABC transporters that regulate K+ channels显示文摘 | Joseph Bryan Alvaro Mu?oz Xinna Zhang Martina Düfer Gisela Drews Peter Krippeit-Drews Lydia Aguilar-Bryan | 2007 | Pflügers Archiv - European Journal of Physiology2007，，5： | 1 |
| 8 | Molecular variation analysis of porcine reproductive and respiratory syndrome virus in China显示文摘 | Lei Zhou Shuxian Chen Jialong Zhang Jingwen Zeng Xin Guo Xinna Ge Dabing Zhang Hanchun Yang | 2009 | Virus Research2009，，1： | 1 |
| 9 | Phylogenetic analysis of porcine epidemic diarrhea virus field strains prevailing recently in China显示文摘 | Yueyi Gao Qiuwen Kou Xinna Ge Lei Zhou Xin Guo Hanchun Yang | 2013 | Archives of Virology2013，，3： | 1 |
| 10 | A putative ABC transporter of Listeria monocytogenes: role in biofilm formation and differential expression profiles by proteomic and microarray显示文摘 | Xinna Zhu Weibing Liu Ren6 Lametsch | 2009 | Abstracts of Food Summit in China 2009 - 6th Annual Meeting of CIFST2009，，： | 1 |
| 11 | A putative ABC transporter is involved in negative regulation of biofilm forma- tion by Listeria monocytogenes 显示文摘 | Xinna Zhu Fei Long Yonghui Chen | 2008 | Appl Environ Microbiol2008，74，24： | 1 |
| 12 | Impaired immunomodulatory function of chronic myeloid leukemia cancer stem cells and the possi- ble mechanism involved in it 显示文摘 | Xishan Z Xinna Z Baoxin H | 2013 | Cancer hnmunol Immunothcr2013，62，4： | 1 |
| 13 | Autophagy sustains the replication of porcine reproductive and respiratory virus in host cells显示文摘 | Qinghao Liu Yixian Qin Lei Zhou Qiuwen Kou Xin Guo Xinna Ge Hanchun Yang Hongbo Hu | 2012 | Virology2012，，2： | 1 |
| 14 | Biofilm formationand food safety in food industries显示文摘 | Xianming Shi Xinna Zhu | 2009 | Trends in Food Science&Technology2009，20，6： | 1 |
| 15 | Biofilm formation and food safety in food industries显示文摘 | Xianming Shi Xinna Zhu | 2009 | Trends in Food Science and Tech- nology2009，20，： | 1 |
| 16 | Autophagy promotes the replication of encephalomyocarditis virus in host cells 显示文摘 | Zhang Y Li Z Xinna G | 2011 | Autophagy2011，7，6： | 1 |
| 17 | Dynamic Analysis and Three-dimensional Finite Element Simulation of Cracked Gear 显示文摘 | Shao Renping Huang Xinna Kaoru Hirota | 2007 | Key Engineering Materials2007，，： | 1 |
| 18 | Comparison of the effects of hu- man adipose and bone marrow mesenchymal stem cells on T lym- phocytes显示文摘 | Xishan Z Baoxin H Xinna Z | 2013 | Cell Biol Int2013，37，1： | 1 |
| 19 | Comparison of the effects of human adipose and bone marrow mesenchymal stem cells on T lymphocytes显示文摘 | Xishan Z Baoxin H Xinna Z | 2013 | Cell Biol Int2013，37，1： | 1 |
| 20 | DEA Analysis of FDI Attractiveness for Sustainable Development : Evi- dence from Chinese Provinces显示文摘 | Ming Lei Xinna Zhao Honghui Deng Keah - Choon Tan | 2013 | Decision Support Systems2013，，56： | 1 |
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