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| 1 | Newborn screening with targeted sequencing:a multicenter investigation and a pilot clinical study in China显示文摘Different newborn screening(NBS) programs have been practiced in many countries since the 1960 s. It is of considerable interest whether next-generation sequencing is applicable in NBS. We have developed a panel of 465 causative genes for 596 early-onset, relatively high incidence, and potentially actionable severe inherited diseases in our Newborn Screening with Targeted Sequencing(NESTS) program to screen 11,484 babies in 8 Women and Children’s hospitals nationwide in China retrospectively. The positive rate from preliminary screening of NESTS was 7.85%(902/11,484). With 45.89%(414/902) follow-up of preliminary positive cases, the overall clinically confirmative diagnosis rate of monogenic disorders was 12.07%(50/414), estimating an average of 0.95%(7.85% × 12.07%) clinical diagnosis rate, suggesting that monogenic disorders account for a considerable proportion of birth defects. The disease/gene spectrum varied in different regions of China. NESTS was implemented in a hospital by screening 3923 newborns to evaluate its clinical application. The turn-around time of a primary report, including the sequencing period of < 7 days, was within 11 days by our automatic interpretation pipeline. Our results suggest that NESTS is feasible and cost-effective as a first-tier NBS program, which will change the status of current clinical practice of NBS in China. | Chanjuan Hao Ruolan Guo Xuyun Hu Zhan Qi Qi Guo Xuanshi Liu Yuanhu Liu Yanhua Sun Xiaofen Zhang Feng Jin Xiujie Wu Ren Cai Dingyuan Zeng Xijiang Hu Xiaohua Wang Xiaoping Ji Wenjie Li Quansheng Xing Lanfang Mu Xiulian Jiang Xue Yang Weimin Yang Yan Zhang Qianli Yin Xin Ni Wei Li | 2022 | Journal of Genetics and Genomics2022，49，1： | 7 |
| 2 | Distribution of airborne SARS-CoV-2 and possible aerosol transmission in Wuhan hospitals, China显示文摘The novel severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has caused an outbreak of CO V ID-19(2019 coronavirus infectious disease)that has led to a global public health crisis.The spread of SARS-CoV-2 can be rapid;but there is still considerable controversy about its main route of transmission[l,2];that is,whether transmission is by contact or through the air[2,3].Recently the W H O(World Health Organization)released a document that recognized the possibility of aerosol transmission of SARS-CoV-2 for indoor environments and enclosed spaces[4].The purpose of the present study was to evaluate the airborne transmission of SARS-CoV-2 through a field investigation of its occurrence in the air and other samples collected from health facilities in W uhan;China. | Jia Hu Chengfeng Lei Zhen Chen Weihua Liu Xujuan Hu Rongjuan Pei Zhengyuan Su Fei Deng Yu Huang Xiulian Sun Junji Cao Wuxiang Guan | 2020 | National Science Review2020，7，12： | 4 |
| 3 | Cloning of biologically active genomes from a Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus isolate by using a bacterial artificial chromosome显示文摘 | Hanzhong Wang Fei Deng Gorben P Pijlman Xinwen Chen Xiulian Sun Just M Vlak Zhihong Hu | 2003 | Virus Research2003，，2： | 1 |
| 4 | Preparation ofgold-labeled antibody probe and its use in immunochromatographyassay for detection of aflatoxin Bj显示文摘 | SUN Xiulian ZHAO Xiaolian TANG Jian | 2005 | International Journal of FoodMicrobiology2005，99，2： | 1 |
| 5 | Generation of low-cadmium rice germplasms via knockout of OsLCD using CRISPR/Cas9显示文摘The OsLCD gene,which has been implicated in cadmium (Cd) accumulation in rice,might be a useful target for CRISPR/Cas9 editing.However,the effects of Os LCD gene editing on Cd accumulation,plant growth,and yield traits remain unknown.Here,we used CRISPR/Cas9to generate oslcd single mutants from indica and japonica rice cultivars.We also generated osnramp5 single mutants and oslcd osnramp5 double mutants in the indica background.When grown in Cd-contaminated paddy soils,all oslcd single mutants accumulated less Cd than the wild types (WTs).Consistent with this,oslcd single mutants grown in Cd-contaminated hydroponic culture accumulated significantly less Cd in the shoots as compared to WTs.This decrease in accumulation probably resulted from the reduction of Cd translocation under Cd stress.Oxidative damage also decreased,and plant growth increased in all oslcd single mutant seedlings as compared to WTs in the presence of Cd.Plant growth and most yield traits,as well essential element concentrations in rice seedling shoots,brown rice,and rice straw,were similar between oslcd single mutants and WTs.In the presence of Cd,Cd concentrations in the brown rice and shoots of oslcd osnramp5 double mutants were significantly decreased compared with WTs as well as osnramp single mutants.Our results suggested that OsL CD knockout may reduce Cd accumulation alone or in combination with other knockout mutations in a variety of rice genotypes;unlike Os Nramp5 mutations,Os LCD knockout did not reduce essential element contents.Therefore,Os LCD knockout might be used to generate low-Cd rice germplasms. | Huamei Chen Rong Ye Ying Liang Shuchang Zhang Xiulian Liu Chongjun Sun Fangbai Li Jicai Yi | 2023 | Journal of Environmental Sciences2023，，4： | 1 |
| 6 | Interactome profiling reveals interaction of SARS-CoV-2 NSP13 with host factor STAT1 to suppress interferon signaling显示文摘Dear Editor,The on-going coronavirus disease 2019(COVID-19)pandemic caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has resulted in u叩rece-dented medical and socioeconomic disruption globally.As of late September 2021,over 231 million confirmed cases have been reported worldwide.Although the viral pathogenesis remains largely unclear,impairment of interferon(IFN)responses likely contributes to disease progression and severity(Blanco-Melo et al.,2020;Meffre and Iwasaki,2020;Min et al.,2021).Indeed,several viral proteins are potential regulators of the IFN system(Min et al.,2021).However,the underlying mechanisms employed by SARS-CoV-2 IFN antagonist candidates,such as nonstructural protein 13(NSP13),still need to be determined. | Kuan Feng Yuan-Qin Min Xiulian Sun Fei Deng Peiqing Li Hualin Wang Yun-jia Ning | 2021 | Journal of Molecular Cell Biology2021，13，10： | 0 |
| 7 | Phosphatidic acid-enabled MKL1 contributes to liver regeneration:Translational implication in liver failure显示文摘Liver regeneration following injury aids the restoration of liver mass and the recovery of liver function.In the present study we investigated the contribution of megakaryocytic leukemia 1(MKL1),a transcriptional modulator,to liver regeneration.We report that both MKL1 expression and its nuclear translocation correlated with hepatocyte proliferation in cell and animal models of liver regeneration and in liver failure patients.Mice with MKL1 deletion exhibited defective regenerative response in the liver.Transcriptomic analysis revealed that MKL1 interacted with E2F1 to program pro-regenerative transcription.MAPKAPK2 mediated phosphorylation primed MKL1 for its interaction with E2F1.Of interest,phospholipase d2 promoted MKL1 nuclear accumulation and liver regeneration by catalyzing production of phosphatidic acid(PA).PA administration stimulated hepatocyte proliferation and enhanced survival in a MKL1-dependent manner in a pre-clinical model of liver failure.Finally,PA levels was detected to be positively correlated with expression of pro-regenerative genes and inversely correlated with liver injury in liver failure patients.In conclusion,our data reveal a novel mechanism whereby MKL1 contributes to liver regeneration.Screening for small-molecule compounds boosting MKL1 activity may be considered as a reasonable approach to treat acute liver failure. | Jiawen Zhou Xinyue Sun Xuelian Chen Huimin Liu Xiulian Miao Yan Guo Zhiwen Fan Jie Li Yong Xu Zilong Li | 2024 | Acta Pharmaceutica Sinica B2024，14，1： | 0 |
| 8 | Comparative transcriptomics provide new insights into the mechanisms by which foliar silicon alleviates the effects of cadmium exposure in rice显示文摘Silicon(Si)has been shown to alleviate Cd stress in rice.Here,we investigated the beneficial effects of foliar Si in an indica rice Huanghuazhan(HHZ).Our results showed that foliar Si in-creases the dry weight and decreases Cd translocation in Cd-exposed rice at the grain-filling stage only,implying that the filling stage is critical for foliar Si to reduce Cd accumulation.We also investigated the transcriptomics in flag leaves(FLs),spikelets(SPs),and node Is(NIs)of Cd-exposed HHZ after foliar Si application at the filling stage.Importantly,the gene expression profiles associated with the Si-mediated alleviation of Cd stress were tissue spe-cific,while shared pathways were mediated by Si in Cd-exposed rice tissues.Furthermore,after the Si treatment of Cd-exposed rice,the ATP-binding cassette(ABC)-transporters were mostly upregulated in FL and SP,while the bivalent cation transporters were mostly down-regulated in FL and NI,possibly helping to reduce Cd accumulation.The genes associated with essential nutrient transporters,carbohydrate and secondary metabolite biosynthesis,and cytochrome oxidase activity were mostly upregulated in Cd-exposed FL and SP,which may help to alleviate oxidative stress and improve plant growth under Cd exposure.Inter-estingly,genes responsible for signal transduction were negatively regulated in FL,but pos-itively regulated in SP,by foliar Si.Our results provide transcriptomic evidence that foliar Si plays an active role in alleviating the effects of Cd exposure in rice.In particular,foliar Si may alter the expression pattern of genes associated with transport,biosynthesis and metabolism,and oxidation reduction. | Chongjun Sun Xiaoyu Liang Xiaomei Gong Huamei Chen Xiulian Liu Shuchang Zhang Fangbai Li Junliang Zhao Jicai Yi | 2022 | Journal of Environmental Sciences2022，34，5： | 0 |
| 9 | Autographa Californica Multiple Nucleopolyhedrovirus orf13 Is Required for Efficient Nuclear Egress of Nucleocapsids显示文摘Autographa californica multiple nucleopolyhedrovirus(Ac MNPV)orf13(ac13)is a conserved gene in all sequenced alphabaculoviruses.However,its function in the viral life cycle remains unknown.In this study,we found that ac13 was a late gene and that the encoded protein,bearing a putative nuclear localization signal motif,colocalized with the nuclear lamina.Deletion of ac13 did not affect viral genome replication,nucleocapsid assembly or occlusion body(OB)formation,but reduced virion budding from infected cells by approximately 400-fold compared with the wild-type virus.Deletion of ac13 substantially impaired the egress of nucleocapsids from the nucleus to the cytoplasm,while the OB morphogenesis was unaffected.Taken together,our results indicated that ac13 was required for efficient nuclear egress of nucleocapsids during virion budding,but was dispensable for OB formation. | Xingang Chen Xiaoqin Yang Chengfeng Lei Fujun Qin Xiulian Sun Jia Hu | 2021 | Virologica Sinica2021，36，5： | 0 |
| 10 | Cis-regulatory decoy disrupts autorepression:a potential escape-resistant anti-viral therapy显示文摘In a recent paper,Chaturvedi et al.published a study in Cell reporting that the artificially synthesized nucleic acid decoys would disrupt the autorepression,which can generate antiviral agents with a high barrier to the evolution of resistance1(Fig.1). | Jia Hu Yuanqin Min Xiulian Sun | 2022 | Signal Transduction and Targeted Therapy2022，7，10： | 0 |
| 11 | Comparative physiological and transcriptomic analyses illuminate common mechanisms by which silicon alleviates cadmium and arsenic toxicity in rice seedlings显示文摘The inessential heavy metal/loids cadmium(Cd)and arsenic(As),which often co-occur in polluted paddy soils,are toxic to rice.Silicon(Si)treatment is known to reduce Cd and As toxicity in rice plants.To better understand the shared mechanisms by which Si alleviates Cd and As stress,rice seedlings were hydroponically exposed to Cd or As,then treated with Si.The addition of Si significantly ameliorated the inhibitory effects of Cd and As on rice seedling growth.Si supplementation decreased Cd and As translocation from roots to shoots,and significantly reduced Cd-and As-induced reactive oxygen species generation in rice seedlings.Transcriptomics analyses were conducted to elucidate molecular mechanisms underlying the Si-mediated response to Cd or As stress in rice.The expression patterns of the differentially expressed genes in Cd-or As-stressed rice roots with and without Si application were compared.The transcriptomes of the Cd-and As-stressed rice roots were similarly and profoundly reshaped by Si application,suggesting that Si may play a fundamental,active role in plant defense against heavy metal/loid stresses by modulating whole genome expression.We also identified two novel genes,0s01g0524500 and 0s06g0514800,encoding a myeloblastosis(MYB)transcription factor and a thionin,respectively,which may be candidate targets for Si to alleviate Cd and As stress in rice,as well as for the generation of Cd-and/or As-resistant plants.This study provides valuable resources for further clarification of the shared molecular mechanisms underlying the Si-mediated alleviation of Cd and As toxicity in rice. | Huiqiong Chen Xiaoyu Liang Xiaomei Gong John R.Reinfelder Huamei Chen Chongjun Sun Xiulian Liu Shuchang Zhang Fangbai Li Chuanping Liu Junliang Zhao Jicai Yi | 2021 | Journal of Environmental Sciences2021，33，11： | 0 |
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