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| 1 | Natural Variation in CCD4 Promoter Underpins Species-Specific Evolution of Red Coloration in Citrus Peel显示文摘Carotenoids and apocarotenoids act as phytohormones and volatile precursors that influence plant development and confer aesthetic and nutritional value critical to consumer preference.Citrus fruits display considerable natural variation in carotenoid and apocarotenoid pigments.In this study,using an integrated genetic approach we revealed that a 5;c/s-regulatory change at CCD4b encoding CAROTENOID CLEAVAGE DIOXYGENASE 4b is a major genetic determinant of natural variation in C3 0 apocarotenoids responsible for red coloration of citrus peel.Functional analyses demonstrated that in addition the known role in synthesizing 3-citraurin,CCD4b is also responsible for the production of another important C3 0 apocarotenoid pigment,p-citraurinene.Furthermore,analyses of the CCD4b promoter and transcripts from various citrus germplasm accessions established a tight correlation between the presence of a putative 5'c/s-regulatory enhancer within an MITE transposon and the enhanced allelic expression of CCD4b in C3 0 apocarotenoid-rich red-peeled accessions.Phylogenetic analysis provided further evidence that functional diversification of CCD4b and naturally occurring variation of the CCD4b promoter resulted in the stepwise evolution of red peels in mandarins and their hybrids.Taken together,our findings provide new insights into the genetic and evolutionary basis of apocarotenoid diversity in plants,and would facilitate breeding efforts that aim to improve the nutritional and aesthetic value of citrus and perhaps other fruit crops. | Xiongjie Zheng Kaijie Zhu Quan Sun Weiyi Zhang Xia Wang Hongbo Cao Meilian Tan Zongzhou Xie Yunliu Zeng Junli Ye Lijun Chai Qiang Xu Zhiyong Pan Shunyuan Xiao Paul D.Fraser Xiuxin Deng | 2019 | Molecular Plant2019，12，9： | 18 |
| 2 | Integration of Metabolomics and Subcellular Organelle Expression Microarray to Increase Understanding the Organic Acid Changes in Post-harvest Citrus Fruit显示文摘Citric acid plays an important role in fresh fruit favor and its adaptability to post-harvest storage conditions.In order to explore organic acid regulatory mechanisms in post-harvest citrus fruit,systematic biological analyses were conducted on stored Hirado Buntan Pummelo(HBP;Citrus grandis)fruits.High-performance capillary electrophoresis,subcellular organelle expression microarray,real-time quantitative reverse transcription polymerase chain reaction,gas chromatography mass spectrometry(GC-MS),and conventional physiological and biochemical analyses were undertaken.The results showed that the concentration of organic acids in HBP underwent a regular fuctuation.GC-MS-based metabolic profling indicated that succinic acid,g-aminobutyric acid(GABA),and glutamine contents increased,but 2-oxoglutaric acid content declined,which further confrmed that the GABA shunt may have some regulatory roles in organic acid catabolism processes.In addition,the concentration of organic acids was signifcantly correlated with senescence-related physiological processes,such as hydrogen peroxide content as well as superoxide dismutase and peroxidase activities,which showed that organic acids could be regarded as important parameters for measuring citrus fruit post-harvest senescence processes. | Xiaohua Sun Andan Zhu Shuzhen Liu Ling Sheng Qiaoli Ma Li Zhang Elsayed Mohamed Elsayed Nishawy Yunliu Zeng Juan Xu Zhaocheng Ma Yunjiang Cheng Xiuxin Deng | 2013 | Journal of Integrative Plant Biology2013，55，11： | 14 |
| 3 | Genome of Wild Mandarin and Domestication History of Mandarin显示文摘官员(柠檬 reticulata ) 是世界范围的最重要的柠檬庄稼之一。它的驯服被相信发生在华南,它是四几千年的官员耕作的中心之一。我们在 Nanling 区域附近收集了官员的自然野人口并且在附近栽培了 landraces。我们发现柠檬性的酸水平戏剧性地在栽培官员被减少。理解 ? 官员驯服的基因基础,我们 de novo 装配了野官员的一个草稿染色体并且分析了一套 104 个柠檬染色体。我们发现 Mangshan 官员是一种原始类型并且二个独立驯服事件发生了,分别地导致在北方和南方 Nanling 山的二组栽培官员(MD1 和 MD2 ) 。二个瓶颈和有效人口尺寸的二扩大为栽培官员的 MD1 组被识别。然而,在 MD2 组织 ? 在人口尺寸有长、连续的减少。MD1 和 MD2 官员从栽培 pummelo 种类显示出种间的基因渗入的不同模式。我们在官员的驯服期间在选择下面可能在基因在柠檬酸盐内容的规定包含了的 aconitate hydratase (ACO ) 识别了高分叉的一个区域,它是。这研究为现存野官员人口的地理起源提供具体基因证据并且使驯服和官员的进化历史清楚些。 | Lun Wang Fa He Yue Huang Jiaxian He Shuizhi Yang Jiwu Zeng Chongling Deng Xiaolin Jiang Yiwen Fang Shaohua Wen Rangwei Xu Huiwen Yu Xiaoming Yang Guangyan Zhong Chuanwu Chen Xiang Yan Changfu Zhou Hongyan Zhang Zongzhou Xie Robert M. Larkin Xiuxin Deng Qiang Xu | 2018 | Molecular Plant2018，11，8： | 14 |
| 4 | Genome-wide Characterization of cis-acting Elements in the Promoters of Key Carotenoid Pathway Genes from the Main Species of Genus Citrus显示文摘Carotenoids are indispensable for both human health and plant survival.Citrus,is one of the fruit crops richest in carotenoid compounds,with approximately 115 kinds of carotenoids;tremendous diversity in carotenoids composition and concentration exists among various species,showing different colors from nearly white to crimson.The carotenoid biosynthetic pathway and the key carotenogenic genes have been identified in citrus;however,the underlying regulatory mechanisms remain unclear.In this study,among the main species of genus Citrus(primitive,wild,and cultivated),we detected carotenoids in flavedo using High-Performance Liquid Chromatography,and analyzed variations in cis-acting elements in the promoters of key carotenoid pathway genes.Intriguingly,both carotenoid composition and content were generally increased during the evolution of citrus,and the corresponding variations in the promoters were identified,including the gain or loss of critical environmental stress-responsive elements and hormone-responsive elements,which are closely associated with carotenoid enhancement.In addition,pummelo has the most heat-responsive elements,but the Mangshan mandarin does not have this element in the promoters of PSY,which is highly related to their geographical origin and indicate that temperature is a critical environmental signal influencing carotenoid accumulation.Moreover,the abscisic acid-responsive motif was rich in almost all the seven species,but the ethylene-responsive motif was deficient,which demystified the unique phytohormone regulation mechanism of carotenoid accumulation in citrus.Overall,our study provides new insights into the molecular regulatory mechanism of carotenoid enhancement in the evolution of citrus,which can facilitate breeding and cultivation efforts to improve the nutritional quality and esthetic value in citrus and hopefully other fruit crops. | Kaijie Zhu Qingjiang Wu Yue Huang Junli Ye Qiang Xu Xiuxin Deng | 2020 | Horticultural Plant Journal2020，6，6： | 8 |
| 5 | Integrated transcriptomic and metabolomic analyses of a wax deficient citrus mutant exhibiting jasmonic acid-mediated defense against fungal pathogens显示文摘Naturally,resistant crop germplasms are important resources for managing the issues of agricultural product safety and environment deterioration.We found a spontaneous mutant of‘Newhall’navel orange(Citrus sinensis Osbeck)(MT)with broad-spectrum protections against fungal pathogens in the orchard,postharvest-storage,and artificial inoculation conditions.To understand the defense mechanism of MT fruit,we constructed a genome-scale metabolic network that integrated metabolome and transcriptome datasets.The coordinated transcriptomic and metabolic data were enriched in two sub-networks,showing the decrease in very long chain fatty acid(by 41.53%)and cuticular wax synthesis(by 81.34%),and increase in the synthesis of jasmonic acid(JA)(by 95.23%)and JA-induced metabolites such as 5-dimethylnobietin(by 28.37%)in MT.Furthermore,cytological and biochemical analyses confirmed that the response to fungal infection in MT was independent of wax deficiency and was correlated with the levels of jasmonates,and the expression of plant defensin gene PDF1.2.Results of exogenous application of MeJA and JA inhibitors such as propyl gallate proved that JA-mediated defense contributes to the strong tolerance against pathogens in MT.Our results indicated that jasmonate biosynthesis and signaling are stimulated by the fatty acid redirection of MT,and participate in the tolerance of pathogenic fungi. | Yizhong He Jingwen Han Runsheng Liu Yuduan Ding Jinqiu Wang Li Sun Xiaoming Yang Yunliu Zeng Weiwei Wen Juan Xu Hongming Zhang Xiang Yan Zhaoxing Chen Zuliang Gu Hong Chen Huanqing Tang Xiuxin Deng Yunjiang Cheng | 2018 | Horticulture Research2018，5，1： | 8 |
| 6 | Systematic review and meta analysis of randomized controlled trials on Tianmagouteng decoction in treatment of primary hypertension with liverYang hyperactivity syndrome显示文摘OBJECTIVE: To assess the efficacy of Tianma-gouteng decoction in the treatment of primary hypertension with liver yang hyperactivity syndrome. METHODS: The databases of MEDLINE (1950-2012), EMbase (1980-2012), China biology medicine (CBM) (1994-2012), China national knowledge infrastructure (CNKI) (1989-2012), Weipu (VIP) (1995-2012) and Wanfang (1989-2012) were searched and we performed manual searches for conference proceedings to select randomized controlled trials (RCTs) on Tianmagouteng decoction for the treatment of primary hypertension with liver Yang hyperactivity syndrome. Studies were enrolled according to the study-selecting criteria. The methodological quality was evaluated, data were extracted and meta-analyses were conducted with RevMan 4.2 software. RESULTS: Nine RCTs were selected involving 784 patients with no significant difference in decrease in systolic blood pressure (SBP) [OR=1.02, 95% CI (-0.24, 2.29), P=0.10] and diastolic blood pressure(DBP) [OR=0.56, 95% CI (-0.10,1.23), P=0.11] between the Tianmagouteng group and the control group. While there was a significantly larger increase in serum superoxide dismutase (SOD)[OR= 6.26, 95% CI (1.27,11.66), P=0. 01] in the Tianma-gouteng group than that in the control group CONCLUSION: Tianmagouteng decoction can decrease both systolic and diastolic blood pressure and greatly increase serum SOD. Due to poor quality of included studies, the efficacy needs to be further assessed. | Xinfang Wu Xiuxin Jiang | 2013 | Journal of Traditional Chinese Medicine2013，33，1： | 8 |
| 7 | Altered expression of mitochondrial related genes in the native Tibetan placents by mitochondrial cDNA array analysis显示文摘Objective: To explore the mechanism of native Tibetan fetuses adaptation to hypoxia, we tried to find the different expression genes about mitochondrial function in the native Tibetan placents. Methods: In this study, the placents of native Tibetan and the high-altitude Han (ha-Han) were collected. After the total RNA extraction, the finally synthesized cDNAs were hybridized to mitochondrial array to find the altered expression genes between them. Then, the cytochrome c oxidase 17 (Cox17), dynactin 2 (DCTN2, also known as p50), and vascular endothelial growth factor receptor (VEGFR, also known as KDR) were chosen from the altered expression genes to further verify the array results using the SYBR Green real-time PCR. Because the altered expression genes (such as Cybb and Cox17) in the array results related to the activities of COXI and COXIV, the placental mitochondria activities of COXI and COXIV were measured to find their changes in the hypoxia. Results: By a standard of ≥1.5 or ≤0.67, there were 24 different expressed genes between the native Tibetan and the ha-Han placents, including 3 up-regulated genes and 21 down-regulated genes. These genes were related to energy metabolism, signal transduction, cell proliferation, electron transport, cell adhesion, nucleotide-excision repair. The array results of Cox17, DCTN2 and KDR were further verified by the real-time RT-PCR. Through the mitochondria respiration measurements, the activity of COXI in the native Tibetan placents were higher than that of ha-Han, there was no difference in COXIV activity between them. Conclusion: The altered mitochondrial related genes in the native Tibetan placents may have a role in the high altitude adaptation for fetuses through changing the activity of mitochondrial COX. | Luo Yongjun Gao Wenxiang Zhao Xiuxin SUO Lang Chen Li Liu Fuyu Song Tonglin Chen Jian Gao Yuqi | 2009 | Journal of Medical Colleges of PLA(China)2009，24，1： | 7 |
| 8 | Difference of a citrus late-ripening mutant (Citrus sinensis) from its parental line in sugar and acid metabolism at the fruit ripening stage显示文摘‘Fengjiewancheng’(FW) (Citrus sinensis), a bud sport of‘Fengjie 72-1’navel orange (FJ), ripens one month later than its parental line. Differences in sugar and acid content and the transcript level of su-crose- and citric-metabolic enzymes for the two cultivars were investigated during fruit ripening. Re-sults showed that both sugar and acid metabolisms of the mutant were affected by the mutation. In the pulp of FW, sugar content was significantly lower than that in FJ before 227 DAF (days after flowering) and higher at 263 DAF; the mutant’s gene expression of one isoform of citrus sucrose synthase (CitSS1) was delayed, and its gene expression of citrus acid invertase (CitAI) was stronger than that in its pa-rental cultivars at 207 and 263 DAF. In the peel, only the sucrose content in FW was significantly lower than those in FJ at the early periods of fruit ripening (165 and 187 DAF); however the transcripts of the sucrose-cleaving enzymes in the mutant were higher than those in FJ at different ripening points. As regards acid accumulation in the two cultivars, it was observed that in the pulp of the mutant, the malic acid content was significantly lower than that in its parental cultivars from 187 to 263 DAF, and in the peel, remarkably higher during the whole fruit ripening period. The citric acid content in both the pulp and the peel of FW was higher than that in those of FJ during the early ripening period and lower during the late ripening period, which were correspondingly associated in part with the higher transcript level of citrus mitochondrial citrate synthase (CitCS) and with lower or undetectable transcript level of citrus cytosolic aconitase (CitAC). Hence, it could be concluded that the mutation in FW affected sugar and acid metabolism, which might be related with other late-ripening phenotypes. | LIU YongZhong, LIU Qing, XIONG JingJing & DENG XiuXin National Key Laboratory of Crop Genetic Improvement College of Horticulture and Forestry Science, Huazhong Agricultural Uni-versity, Wuhan 430070, China | 2007 | Science China(Life Sciences)2007，50，4： | 5 |
| 9 | Self-Compensating Displacement Sensor Based on Hydramatic Structured Transducer and Fiber Bragg Grating显示文摘有为同时的排水量和温度测量的一个大测量范围的一个光纤维排水量传感器在这份报纸被介绍。我们基于活塞和 hydraumatic 结构开发了一个特定的变换器认识到大排水量大小,它把大测量范围和高精确合为一个单个传感器系统。光谱显示出过去常补偿的二座思考山峰在排水量察觉跨敏感。这个排水量传感器能线性地与 0.036 nm/mm 的高敏感在比 45 公里大的一个大测量排水量范围工作。我们报导了的传感器能为即时排水量在象监视的机械形状或液体水平那样的许多工业环境监视被开发。 | Shimeng CHEN Yun LIU Xiuxin LIU Yang ZHANG Wei PENG | 2015 | Photonic Sensors2015，5，4： | 5 |
| 10 | Genome of a citrus rootstock and global DNA demethylation caused by heterografting显示文摘Grafting is an ancient technique used for plant propagation and improvement in horticultural crops for at least 1,500 years.Citrus plants,with a seed-to-seed cycle of 5–15 years,are among the fruit crops that were probably domesticated by grafting.Poncirus trifoliata,a widely used citrus rootstock,can promote early flowering,strengthen stress tolerance,and improve fruit quality via scion–rootstock interactions.Here,we report its genome assembly using PacBio sequencing.We obtained a final genome of 303 Mb with a contig N50 size of 1.17Mb and annotated 25,680 protein-coding genes.DNA methylome and transcriptome analyses indicated that the strong adaptability of P.trifoliata is likely attributable to its special epigenetic modification and expression pattern of resistance-related genes.Heterografting by using sweet orange as scion and P.trifoliata as rootstock and autografting using sweet orange as both scion and rootstock were performed to investigate the genetic effects of the rootstock.Single-base methylome analysis indicated that P.trifoliata as a rootstock caused DNA demethylation and a reduction in 24-nt small RNAs(sRNAs)in scions compared to the level observed with autografting,implying the involvement of sRNA-mediated graft-transmissible epigenetic modifications in citrus grafting.Taken together,the assembled genome for the citrus rootstock and the analysis of graft-induced epigenetic modifications provide global insights into the genetic effects of rootstock–scion interactions and grafting biology. | Yue Huang Yuantao Xu Xiaolin Jiang Huiwen Yu Huihui Jia Chunming Tan Gang Hu Yibo Hu Muhammad Junaid Rao Xiuxin Deng Qiang Xu | 2021 | Horticulture Research2021，8，1： | 4 |
| 11 | Microarray Expression Profiling of Postharvest Ponkan Mandarin (Citrus reticulata) Fruit under Cold Storage Reveals Regulatory Gene Candidates and Implications on Soluble Sugars Metabolism显示文摘Low temperature storage is widely applied to maintain citrus postharvest fruit quality.In this study,the transcriptional and metabolic changes in the pulp tissue of Citrus reticulata Blanco cv.'Ponkan' were studied for three successive months under cold storage by Affymetrix Citrus GeneChip and gas chromatography,respectively.As many as 2 161 differentially expressed transcripts were identified based on the bayesian hierarchical model.The statistical analysis of gene ontology revealed that defense/stress-related genes were induced quickly,while autophagy-related genes were overrepresented in the late sampling stages,suggesting that the functional shift may coincide with the subsequent steps of chilling development.We further classified the potential regulatory components and concluded that ethylene may play the crucial role in chilling development in this non-climacteric fruit.To cope with complex events,53 upregulated transcription factors represented regulatory candidates.Within these,the AP2-EREBP,C2H2 and AS2 gene family were overrepresented.Cold storage also causes alterations in various metabolic pathways;a keen interest is paid in deciphering expression changes of soluble sugar genes as increased evidence that soluble sugars act as both osmolytes and metabolite signal molecules.Our results will likely facilitate further studies in this field with promising genetic candidates during chilling. | Andan Zhu Wenyun Li Junli Ye Xiaohua Sun Yuduan Ding Yunjiang Cheng Xiuxin Deng | 2011 | Journal of Integrative Plant Biology2011，53，5： | 3 |
| 12 | An efficient protocol for genomic DNA extraction fromCitrus species显示文摘 | Yun-Jiang Cheng Wen-Wu Guo Hua-Lin Yi Xiao-Min Pang Xiuxin Deng | 2003 | Plant Molecular Biology Reporter2003，，2： | 2 |
| 13 | Lipidomic and transcriptomic analysis reveals reallocation of carbon flux from cuticular wax into plastid membrane lipids in a glossy“Newhall”navel orange mutant显示文摘Both cuticle and membrane lipids play essential roles in quality maintenance and disease resistance in fresh fruits.Many reports have indicated the modification of alternative branch pathways in epicuticular wax mutants;however,the specific alterations concerning lipids have not been clarified thus far.Here,we conducted a comprehensive,timeresolved lipidomic,and transcriptomic analysis on the“Newhall”navel orange(WT)and its glossy mutant(MT)“Gannan No.1”.The results revealed severely suppressed wax formation accompanied by significantly elevated production of 36-carbon plastid lipids with increasing fruit maturation in MT.Transcriptomics analysis further identified a series of key functional enzymes and transcription factors putatively involved in the biosynthesis pathways of wax and membrane lipids.Moreover,the high accumulation of jasmonic acid(JA)in MT was possibly due to the need to maintain plastid lipid homeostasis,as the expression levels of two significantly upregulated lipases(CsDAD1 and CsDALL2)were positively correlated with plastid lipids and characterized to hydrolyze plastid lipids to increase the JA content.Our results will provide new insights into the molecular mechanisms underlying the natural variation of plant lipids to lay a foundation for the quality improvement of citrus fruit. | Haoliang Wan Hongbo Liu Jingyu Zhang Yi Lyu Zhuoran Li Yizhong He Xiaoliang Zhang Xiuxin Deng Yariv Brotman Alisdair RFernie Yunjiang Cheng Weiwei Wen | 2020 | Horticulture Research2020，7，1： | 2 |
| 14 | RAPD-based genetic analysis of offsprings from the sexual cross using allotetraploid citrus somatic hybrid as pollen parent显示文摘Thirty-one polymorphic decamer primers were selected to genotype 92 progenies from the cross be- tween Yiben No.4, a monoembryonic diploid F1 hybrid of Citrus reticulata Blanco cv Huanongbendizao tangerine and C. ichangensis Swingle, and [Hamlin sweet orange + Rough lemon], an allotetraploid somatic hybrid of Citrus sinensis Osbeck cv. Hamlin and C. jambhiri Lush cv. Rough Lemon. χ2 (Chi-square) analysis of RAPD markers in the progenies indicated they were randomly transmitted from the four donor parents, without significant difference between the diploids and triploids. However, these progenies were clustered into three major groups using dendrogram constructed by UPGMA, skewed to three parents in certain degrees, 15 (13 triploids and 2 diploids) to Hamlin, 16 (9 and 7) to Yiben No. 4, and 61 (57 and 4) to [Hamlin sweet orange + Rough Lemon] from which genomic contribu- tion was predominant in progenies, respectively. | YI HuaLin & DENG XiuXin State Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, National Center of Citrus Breeding, Huazhong Agricultural University, Wuhan 430070, China | 2007 | Science China(Life Sciences)2007，50，3： | 2 |
| 15 | Down regulated expression of S_(2)-RNase attenuates self-incompatibility in “Guiyou No.1”pummelo显示文摘Self-incompatibility(SI)substantially restricts the yield and quality of citrus.Therefore,breeding and analyzing selfcompatible germplasm is of great theoretical and practical signi ficance for citrus.Here,we focus on the mechanism of a self-compatibility mutation in‘Guiyou No.1'pummelo(Citrus maxima),which is a spontaneous mutant of‘Shatian’pummelo(Citrus maxima,self-incompatibility).The rate of fruit set and the growth of pollen tubes in the pistil con firmed that a spontaneous mutation in the pistil is responsible for the self-compatibility of‘Guiyou No.1'.Segregation ratios of the S genotype in progeny,expression analysis,and western blotting validated that the reduced levels of S_(2)-RNase mRNA contribute to the loss of SI in‘Guiyou No.1'.Furthermore,we report a phased assembly of the‘Guiyou No.1'pummelo genome and obtained two complete and well-annotated S haplotypes.Coupled with an analysis of SV variations,methylation levels,and gene expression,we identi fied a candidate gene(CgHB40),that may in fluence the regulation of the S/^RNase promoter.Our data provide evidence that a mutation that affects the pistilled to the loss of SI in‘Guiyou No.1'by in fluencing a poorly understood mechanism that affects transcriptional regulation.This work signi ficantly advances our understanding of the genetic basis of the SI system in citrus and provides information on the regulation of S-RNase genes. | ianbing Hu Qiang Xu Chenchen Liu Binghao Liu Chongling Deng Chuanwu Chen Zhuangmin Wei Muhammad Husnain Ahmad Kang Peng Hao Wen Xiangling Chen Peng Chen Robert MLarkin Junli Ye Xiuxin Deng Lijun Chai | 2021 | Horticulture Research2021，8，1： | 2 |
| 16 | Multiomics-based dissection of citrus flavonoid metabolism using a Citrus reticulata × Poncirus trifoliata population显示文摘Deciphering the genetic basis of plant secondary metabolism will provide useful insights for genetic improvement and enhance our fundamental understanding of plant biological processes.Although citrus plants are among the most important fruit crops worldwide,the genetic basis of secondary metabolism in these plants is largely unknown.Here,we use a high-density linkage map to dissect large-scale flavonoid metabolic traits measured in different tissues(young leaf,old leaf,mature pericarp,and mature pulp)of an F_(1) pseudo-testcross citrus population.We detected 80 flavonoids in this population and identified 138 quantitative trait loci(QTLs)for 57 flavonoids in these four tissues.Based on transcriptional profiling and functional annotation,twenty-one candidate genes were identified,and one gene encoding flavanone 3-hydroxylase(F3H)was functionally verified to result in naturally occurring variation in dihydrokaempferol content through genetic variations in its promoter and coding regions.The abundant data resources collected for diverse citrus germplasms here lay the foundation for complete characterization of the citrus flavonoid biosynthetic pathway and will thereby promote efficient utilization of metabolites in citrus quality improvement. | Jiaolin Mou Zhehui Zhang Haiji Qiu Yang Lu Xiang Zhu Ziquan Fan Qinghua Zhang Junli Ye Alisdair R.Fernie Yunjiang Cheng Xiuxin Deng Weiwei Wen | 2021 | Horticulture Research2021，8，1： | 2 |
| 17 | Genetic diversity and relatedness analysis of nine wild species of tree peony based on simple sequence repeats markers显示文摘Tree peony has nine wild species,but the evolutionary relationship of them is still unclear.Here,a total of 274 specimens from 22 natural populations of nine wild species were collected,and their genetic diversity and similarity was analyzed based on Simple Sequence Repeats(SSR)molecular markers.A total of 106 alleles were generated based on 20 primers and with an average of 5.3 alleles per primer.Shannon’s information index(I)ranged from 0.6333 to 1.7842,and the average of Nei’s genetic diversity coefficient(H)was 0.5771.Polymorphism Information Content(PIC)value varied from 0.29 to 0.77,ten of these primers had high polymorphism(PIC≥0.50).All the above genetic parameters of primers reflect more rich genetic diversity information compared with other researches using SSR molecular markers to study the genetic diversity of tree peony wild species.At the population level,the lowest and highest degree of genetic diversity occurred in Paeonia ludlowii-P1 and P.delavayi-P3 population,respectively.Whereas at species level,the genetic diversity of 9 wild peony species was as follows:P.lutea>P.delavayi>P.rockii>P.qiui>P.ostii>P.decomposita>P.potaninii>P.spontanea>P.ludlowii.Furthermore,cluster analysis at species level divided the nine wild tree peony species into two branches.In branch I,the closest phylogenetic relationship was found between P.ostii and P.rockii,followed by P.spontanea,P.qiui,and P.decomposita.In branch II,the closest relationship occurred between P.lutea and P.delavayi,followed by P.potaninii and P.ludlowii.Clustering results supported the division of tree peonies into two subsects(Delavayanae and Vaginatae),it also supported P.potaninii and P.ludlowii as independent species.The results provided novel insight into the genetic diversity and phylogenetic relationship of nine wild tree peony species.It will help formulate comprehensive protection measures of wild germplasm resources and select proper parents for distant hybridization in the future. | Yuqian Xue Rong Liu Jingqi Xue Shunli Wang Xiuxin Zhang | 2021 | Horticultural Plant Journal2021，7，6： | 1 |
| 18 | A meta-analysis of artificial total disc replacement versus fusion for lumbar degenerative disc disease显示文摘 | Wu Yajun Zhu Yue Han Xiuxin Cui Cui | 2010 | European Spine Journal2010，，8： | 1 |
| 19 | Pro-inflammatory effects of a litchi protein extract in murine RAW264.7 macrophages显示文摘It has been observed that the consumption of litchi often causes symptoms characterized by itching or sore throat,gum swelling,oral cavity ulcers and even fever and inflammation,which significantly impair the quality of life of a large population.Using the RAW264.7 cell line,a step-by-step strategy was used to screen for the components in litchi fruits that elicited adverse reactions.The adverse reaction fractions were identified by mass spectrometry and analyzed using the SMART program,and a sequence alignment of the homologous proteins was performed.MTT tests were used to determine the cytotoxicity of a litchi protein extract in RAW264.7 macrophages,and real-time PCR was applied to analyze the expression of inflammatory genes in the RAW264.7 cells treated with lipopolysaccharide or the litchi protein extract.The results showed that the litchi water-soluble protein extract could increase the production of the pro-inflammatory mediators IL-1β,iNOS and COX-2,and the anti-inflammatory mediator HO-1 in the RAW264.7 cell line.The 14-3-3-like proteins GF14 lambda,GF14 omega and GF14 upsilon were likely the candidate proteins that caused the adverse effects. | Xiaoli Wang Xiaorong Hu Huiqing Yan Zhaocheng Ma Xiuxin Deng | 2016 | Horticulture Research2016，3，1： | 1 |
| 20 | A meta-analysis of artificial total disc replacement versus fusion for lumbar degenerative disc disease 显示文摘 | Yajun W Yue Z Xiuxin H | 2010 | Eur Spine J2010，19，8： | 1 |
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