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| 1 | Research advances in the detection of miRNA显示文摘MicroRNAs (miRNAs) are a family of endogenous, small (approximately 22 nucleotides in length), noncoding, functional RNAs. With the development of molecular biology, the research of miRNA biological function has attracted significant interest, as abnormal miRNA expression is identified to contribute to serious human diseases such as cancers. Traditional methods for miRNA detection do not meet current demands. In particular, nanomaterial-based methods, nucleic acid amplification-based methods such as rolling circle amplification (RCA), loop-mediated isothermal amplification (LAMP), strand-displacement amplification (SDA) and some enzyme-free amplifications have been employed widely for the highly sensitive detection of miRNA. MiRNA functional research and clinical diagnostics have been accelerated by these new techniques. Herein, we summarize and discuss the recent progress in the development of miRNA detection methods and new applications. This review will provide guidelines for the development of follow-up miRNA detection methods with high sensitivity and specificity, and applicability to disease diagnosis and therapy. | Jiawei Ye Mingcheng Xu Xueke Tian Sheng Cai Su Zeng | 2019 | Journal of Pharmaceutical Analysis2019，9，4： | 15 |
| 2 | Regional distribution and diurnal variation of deep convective systems over the Asian monsoon region显示文摘Using 12 years of data from the Tropical Rainfall Measuring Mission(TRMM)-based Precipitation Radar(PR),spatial and diurnal variations of deep convective systems(DCSs)over the Asian monsoon region are analyzed.The DCSs are defined by a 20 dBZ echo top extending 14 km.The spatial distribution of DCSs genesis is also discussed,with reference to the National Centers for Environmental Prediction(NCEP)reanalysis data.The results show that DCSs occur mainly over land.They concentrate in south of 20°N during the pre-monsoon season,and then move distinctly to mid-latitude regions,with the most active region on the south slope of the Himalayas during monsoon season.DCSs over the Tibetan Plateau are more frequent than those in central-eastern China,but smaller in horizontal scale and weaker in convective intensity.DCSs in central-eastern China have more robust updrafts and generate more lightning flashes than in other Asian monsoon regions.The horizontal scale of DCSs over the ocean is larger than that over the other regions,and the corresponding minimum infrared(IR)brightness temperature is lower,whereas the convective intensity is weaker.Continental DCSs are more common from noon through midnight,and DCSs over the Tibetan Plateau are more frequently from noon through evening.Oceanic DCSs frequency has a weaker diurnal cycle with dawn maximum,and diurnal variation of DCSs over the tropical maritime continent is consistent with that over the continent. | WU XueKe QIE XiuShu YUAN Tie | 2013 | Science China Earth Sciences2013，56，5： | 9 |
| 3 | Glycolysis reprogramming in cancer-associated fibroblasts promotes the growth of oral cancer through the lncRNA H19/miR-675-5p/PFKFB3 signaling pathway显示文摘As an important component of the tumor microenvironment,cancer-associated fibroblasts(CAFs)secrete energy metabolites to supply energy for tumor progression.Abnormal regulation of long noncoding RNAs(lncRNAs)is thought to contribute to glucose metabolism,but the role of lncRNAs in glycolysis in oral CAFs has not been systematically examined.In the present study,by using RNA sequencing and bioinformatics analysis,we analyzed the lncRNA/mRNA profiles of normal fibroblasts(NFs)derived from normal tissues and CAFs derived from patients with oral squamous cell carcinoma(OSCC).LncRNA H19 was identified as a key lncRNA in oral CAFs and was synchronously upregulated in both oral cancer cell lines and CAFs.Using small interfering RNA(siRNA)strategies,we determined that lncRNA H19 knockdown affected proliferation,migration,and glycolysis in oral CAFs.We found that knockdown of lncRNA H19 by siRNA suppressed the MAPK signaling pathway,6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3(PFKFB3)and miR-675-5p.Furthermore,the lncRNA H19/miR-675-5p/PFKFB3 axis was involved in promoting the glycolysis pathway in oral CAFs,as demonstrated by a luciferase reporter system assay and treatment with a miRNA-specific inhibitor.Our study presents a new way to understand glucose metabolism in oral CAFs,theoretically providing a novel biomarker for OSCC molecular diagnosis and a new target for antitumor therapy. | Jin Yang Xueke Shi Miao Yang Jingjing Luo Qinghong Gao Xiangjian Wang Yang Wu Yuan Tian Fanglong Wu Hongmei Zhou | 2021 | International Journal of Oral Science2021，13，1： | 8 |
| 4 | Degradation of carbon tetrachloride in thermally activated persulfate system in the presence of formic acid显示文摘碳四氯化物(CT ) 的热地激活的 persulfate (PS ) 降级面对蚁的酸(FA ) 被调查。结果显示那 CT 降级跟随了零份订单运动模型,和公司 2- | Minhui XU Xiaogang GU Shuguang LU Zhouwei MIAO Xueke ZANG Xiaoliang WU Zhaofu QIU Qian SUI | 2016 | Frontiers of Environmental Science & Engineering2016，10，3： | 6 |
| 5 | Characterization of 500 Chinese patients with cervical esophageal cancer by clinicopathological and treatment outcomes显示文摘Objective: There are no comprehensive studies on survival outcomes and optimal treatment protocols for cervical esophageal cancer(CEC), due to its rare clinical prevalence. Our objective was to determine the relationship between pathological characteristics, treatment protocols, and survival outcomes in Chinese CEC patients.Methods: A total of 500 Chinese CEC patients were selected from our 500,000 esophageal and gastric cardia carcinoma database(1973–2018). There were two main groups: patients treated with surgery, and patients receiving non-surgical treatments(radiotherapy, radiochemotherapy, and chemotherapy). The Chi-square test and Kaplan–Meier method were used to compare the continuous variables and survival.Results: Among the 500 CEC patients, 278(55.6%) were male, and the median age was 60.9 ± 9.4 years. A total of 496 patients(99.2%) were diagnosed with squamous cell carcinoma. In 171(34.2%) patients who received surgery, 22(12.9%) had undergone laryngectomy. In 322(64.4%) patients who received non-surgical treatments, 245(76.1%) received radiotherapy. Stratified survival analysis showed that only T stage was related with survival outcomes for CEC patients in the surgical group, and the outcomes between laryngectomy and non-laryngectomy patients were similar. It was noteworthy that the 5-year survival rate was similar in CEC patients among the different groups treated with surgery, radiotherapy, chemotherapy, or radiochemotherapy(P = 0.244). Conclusions: The CEC patients had similar survival outcomes after curative esophagectomy and radiotherapy, including those with or without total laryngectomy. These findings suggest that radiotherapy could be the initial choice for treatment of Chinese CEC patients. | Peinan Chen Xueke Zhao Fuyou Zhou Xin Song Shoujia Hu Yan Jin Xianzeng Wang Xuena Han ZongminFan Ran Wang Bei Li Wenli Han Panpan Wang Jilin Li Lixin Wan Liguo Zhang Qide Bao Fubao Chang Yanru Qin Zhiwei Chang Jianwei Ku Haijun Yang Ling Yuan Jingli Ren Xuemin Li Lidong Wang | 2020 | Cancer Biology & Medicine2020，17，1： | 6 |
| 6 | Mitochondrial malic enzyme 2 promotes breast cancer metastasis via stabilizing HIF-1α under hypoxia显示文摘Objective: α-ketoglutarate(α-KG) is the substrate to hydroxylate collagen and hypoxia-inducible factor-1α(HIF-1α), which are important for cancer metastasis. Previous studies have shown that the upregulation of collagen prolyl 4-hydroxylase in breast cancer cells stabilizes the expression of HIF-1α by depleting α-KG levels. We hypothesized that mitochondrial malic enzyme 2(ME2) might also affect HIF-1α expression via modulating α-KG levels in breast cancer cells.Methods: We evaluated ME2 protein expression in 100 breast cancer patients using immunohistochemistry and correlated with clinicopathological indicators. The effect of ME2 knockout on cancer metastasis was evaluated using an orthotopic breast cancer model. The effect of ME2 knockout or knockdown on the levels of α-KG and HIF-1α proteins in breast cancer cell lines was determined both in vitro and in vivo.Results: ME2 was found to be upregulated in the human breast cancerous tissues compared with the matched precancerous tissues(P<0.001). The elevated expression of ME2 was associated with a poor prognosis(P=0.019).ME2 upregulation was also related to lymph node metastasis(P=0.016), pathological staging(P=0.033), and vascular cancer embolus(P=0.014). Also, ME2 knockout significantly inhibited lung metastasis in vivo. In the tumors formed by ME2 knockout cells, the levels of α-KG were significantly increased and collagen hydroxylation level did not change significantly but HIF-1α protein expression was significantly decreased, compared to the control samples. In cell culture, cells with ME2 knockout or knockdown demonstrated significantly higher α-KG levels but significantly lower HIF-1α protein expression than control cells under hypoxia. Exogenous malate and α-KG exerted similar effect on HIF-1α in breast cancer cells to ME2 knockout or knockdown. Additionally,treatment with malate significantly decreased 4 T1 breast cancer lung metastasis. ME2 expression was associated with HIF-1α levels in human breast cancer samples(P=0.008).Conclusions: Our results provide evidence that upregulation of ME2 is associated with a poor prognosis of breast cancer patients and propose a mechanistic understanding of a link between ME2 and breast cancer metastasis. | Duo You Danfeng Du Xueke Zhao Xinmin Li Minfeng Ying Xun Hu | 2021 | Chinese Journal of Cancer Research2021，33，3： | 3 |
| 7 | Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering显示文摘A close relationship has been reported to exist between cadherin-mediated cell-cell adhesion and integrin-mediated cell mobility,and protein tyrosine phosphatase 1B(PTP1B)may be involved in maintaining this homeostasis.The stable residence of mesenchymal stem cells(MSCs)and endothelial cells(ECs)in their niches is closely related to the regulation of PTP1B.However,the exact role of the departure of MSCs and ECs from their niches during bone regeneration is largely unknown.Here,we show that the phosphorylation state of PTP1B tyrosine-152(Y152)plays a central role in initiating the departure of these cells from their niches and their subsequent recruitment to bone defects.Based on our previous design of a PTP1B Y152 region-mimicking peptide(152RM)that significantly inhibits the phosphorylation of PTP1B Y152,further investigations revealed that 152RM enhanced cell migration partly via integrinαvβ3 and promoted MSCs osteogenic differentiation partly by inhibiting ATF3.Moreover,152RM induced type H vessels formation by activating Notch signaling.Demineralized bone matrix(DBM)scaffolds were fabricated with mesoporous silica nanoparticles(MSNs),and 152RM was then loaded onto them by electrostatic adsorption.The DBM-MSN/152RM scaffolds were demonstrated to induce bone formation and type H vessels expansion in vivo.In conclusion,our data reveal that 152RM contributes to bone formation by coupling osteogenesis with angiogenesis,which may offer a potential therapeutic strategy for bone defects. | Yong Tang Keyu Luo Yin Chen Yueqi Chen Rui Zhou Can Chen Jiulin Tan Moyuan Deng Qijie Dai Xueke Yu Jian Liu Chengmin Zhang Wenjie Wu Jianzhong Xu Shiwu Dong Fei Luo | 2021 | Bioactive Materials2021，6，7： | 3 |
| 8 | Three-dimensional reconstruction of Picea wilsonii Mast. pollen grains using automated electron microscopy显示文摘Three-dimensional electron microscopy(3 D-EM) has attracted considerable attention because of its ability to provide detailed information with respect to developmental analysis. However, large-scale high-resolution 3 D reconstruction of biological samples remains challenging. Herein, we present a 3 D view of a Picea wilsonii Mast. pollen grain with 100 nm axial and38.57 nm lateral resolution using AutoCUTS-SEM(automatic collector of ultrathin sections-scanning electron microscopy). We established a library of 3,127 100 nm thick serial sections of pollen grains for preservation and observation, demonstrating that the protocol can be used to analyze large-volume samples. After obtaining the SEM images, we reconstructed an entire pollen grain comprising 734 serial sections. The images produced by 3D reconstruction clearly revealed the main components of the P.wilsonii pollen grain, i.e., two sacci and pollen corpus, tube cell, generative cell, and two degenerated prothallial cells, and their internal organization. In addition, we performed a quantitative analysis of the different pollen grain cells, including sacci, and found that there were 202 connections within a saccus SEM image. Thus, for the first time, this study provided a global 3D view of the entire pollen grain, which will be useful for analyzing pollen development and growth. | Weiwei Shen Lingyu Ma Xi Zhang Xixia Li Yuanyuan Zhao Yanping Jing Yun Feng Xueke Tan Fei Sun Jinxing Lin | 2020 | Science China(Life Sciences)2020，63，2： | 3 |
| 9 | Blue-detuned optical atom trapping in a compact plasmonic structure显示文摘We theoretically propose blue-detuned optical trapping for neutral atoms via strong near-field interfacing in a plasmonic nanohole array. The optical field at resonance forms a nanoscale-trap potential with an FWHM of 200 nm and about ~370 nm away from the nanohole; thus, a stable 3 D atom trapping independent of the surface potential is demonstrated. The effective trap depth is more than 1 m K when the optical power of trapping light is only about 0.5 m W, while the atom scattering rate is merely about 3.31 s^(-1), and the trap lifetime is about 800 s.This compact plasmonic structure provides high uniformity of trap depths and a two-layer array of atom nanotraps, which should have important applications in the manipulation of cold atoms and collective resonance fluorescence. | ZHAO CHEN FAN ZHANG QI ZHANG JUANJUAN REN HE HAO XUEKE DUAN PENGFEI ZHANG TIANCAI ZHANG YING GU QIHUANG GONG | 2017 | Photonics Research2017，5，5： | 2 |
| 10 | Predicting the Potential Distribution of Rubia cordifolia L.in China显示文摘[Objectives]We intend to conduct a study to predict habitat suitability and geographic distribution of Rubia cordifolia L.,to find the most suitable ecological environment for its growth,and to provide a theoretical basis for the development and cultivation of R.cordifolia L.industry.[Methods]Based on the environmental factors and the occurrence data,we predicted the potential geographic distribution of R.cordifolia L.in China by combining the MaxEnt model and ArcGIS.[Results]The training set and test set AUC were respectively 0.963 and 0.946,indicating that the MaxEnt model predicted well.The wettest monthly precipitation,precipitation in July,precipitation of driest season,and average monthly temperature in September and April were the dominant environmental factors affecting the distribution of R.cordifolia L.The highly habitable areas were mainly concentrated in central,eastern and northern China.[Conclusions]The results of this study can be used as a reference for the development of R.cordifolia L.industry in related areas. | Wei QIN Suping JIANG Xueke ZHANG Dingkang CHEN Shoujin LIU Daiyin PENG | 2021 | Medicinal Plant2021，12，3： | 2 |
| 11 | Experimental and numerical study on improvement performance by wave parallel flow field in a proton exchange membrane fuel cell显示文摘The performance and operation stability of proton exchange membrane fuel cells(PEMFCs)are closely related to the transportation of reactants and water management in the membrane electrode assembly(MEA)and flow field.In this paper,a new three-dimensional wave parallel flow field(WPFF)in cathode was designed and analyzed throughout simulation studies and an experimental method.The experimental results show that the performance of PEMFC with WPFF outperforms that of PEMFC with straight parallel flow field(SPFF).Specifically,the peak power density increased by 13.45%for the PEMFC with WPFF as opposed to PEMFC with SPFF.In addition,the flow field with area of 11.56 cm^(2) was formed by the assembly of transparent end plate used for cathode and the traditional graphite plate used for anode.To understand the mechanism of the novel flow field improving the performance of PEMFC,a model of PEMFC was proposed based on the geometry,operating conditions and MEA parameters.The thickness of gas diffusion layers(GDL),catalytic layers(CL)and proton exchange membrane were measured by scanning electron microscope.The simulation result shows that compared with SPFF,the WPFF based PEMFC promote the oxygen transfer from flow channel to the surface of CL through GDL,and it was beneficial to remove the liquid water in the flow channel and the MEA. | Zijun Li Shubo Wang Sai Yao Xueke Wang Weiwei Li Tong Zhu Xiaofeng Xie | 2022 | Chinese Journal of Chemical Engineering2022，35，5： | 2 |
| 12 | Insights into wing dimorphism in worldwide agricultural pest and hostalternating aphid Aphis gossypii显示文摘Background:The worldwide pest Aphis gossypii has three-winged morphs in its life cycle,namely,winged parthenogenetic female(WPF),winged gynopara(GP),and winged male,which are all produced by a wingless parthenogenetic female(WLPF).Most studies on A.gossypii have focused on WPF,while few have investigated GP and male.The shared molecular mechanism underlying the wing differentiation in the three wing morphs of A.gossypii remains unknown.The wing differentiation of WPF was explored in a previous study.Herein,GP and male were induced indoors.The characters of the body,internal genitals,wing veins,and fecundity of GP and male were compared with those of WPF or WLPF.Compared with WLPF,the shared and separate differentially expressed genes(DEGs)were identified in these three-wing morphs.Results:Newly-born nymphs reared in short photoperiod condition(8 L:16D,18°C)exclusively produced gynoparae(GPe)and males in adulthood successively,in which the sex ratio was GP biased.A total of 14 GPe and 9 males were produced by one mother aphid.Compared with WLPF,the three-wing morphs exhibited similar morphology and wing vein patterns but were obviously discriminated in the length of fore-and underwings,reproductive system,and fecundity.A total of 37090 annotated unigenes were obtained from libraries constructed using the four morphs via RNA sequencing(RNA-Seq).In addition,10867 and 19334 DEGs were identified in the pairwise comparison of GP versus WLPF and male versus WLPF,respectively.Compared with WLPF,the winged morphs demonstrated 2335 shared DEGs(1658 upregulated and 677 downregulated).The 1658 shared upregulated DEGs were enriched in multiple signaling pathways,including insulin,FoxO,MAPK,starch and sucrose metabolism,fatty acid biosynthesis,and degradation,suggesting their key roles in the regulation of wing plasticity in the cotton aphid.Forty-four genes that spanned the range of differential expression were chosen to validate statistical analysis based on RNA-Seq through the reverse transcription quantitative real time polymerase chain reaction(RT-qPCR).The comparison concurred with the expression pattern(either up-or downregulated)and supported the accuracy and reliability of RNA-Seq.Finally,the potential roles of DEGs related to the insulin signaling pathway in wing dimorphism were discussed in the cotton aphid.Conclusions:The present study established an efficiently standardized protocol for GP and male induction in cotton aphid by transferring newly-born nymphs to short photoperiod conditions(8 L:16D,18°C).The external morphological characters,especially wing vein patterns,were similar among WPFs,GPe,and males.However,their reproductive organs were strikingly different.Compared with WLPF,shared(2335)and exclusively(1470 in WLPF,2419 in GP,10774 male)expressed genes were identified in the three-wing morphs through RNA-Seq,and several signaling pathways that are potentially involved in their wing differentiation were obtained,including insulin signaling,starch and sucrose metabolism. | JI Jichao HUANGFU Ningbo LUO Junyu GAO Xueke NIU Lin ZHANG Shuai CUI Jinjie | 2021 | Journal of Cotton Research2021，4，1： | 2 |
| 13 | Numerical Simulation on a Nanosecond-Pulse Surface Dielectric Bar- rier Discharge Actuator in Near Space 显示文摘 | Xueke Che Tao Shao Wansheng Nie | 2012 | Journal ofPhysics D : Applied Physics2012，45，： | 1 |
| 14 | Effect ofchromium intermediate layer on properties of silver coatings显示文摘 | Xu Xueke Tang Zhaosheng Fan Zhengxiu | 2004 | Optical Engineering2004，43，4： | 1 |
| 15 | Interactions of miR - 34b / c and TP - 53 polymorphisms on the risk of nasopharyngeal carcinoma显示文摘 | Lijuan Li Jian Wu Xiutian Sima Peng Bai Wei Deng Xueke Deng Lin Zhang Linbo Gao | 2013 | Tumor Biology2013，，3： | 1 |
| 16 | DNA repair gene XRCC1 and XPD polymorphisms and risk of lung cancer in a Chinese population显示文摘 | Chen S TangD XueK | 2002 | Carcinogenesis2002，23，8： | 1 |
| 17 | Effect of Maotai liquor on P53 gene in primary hepatocellular carcinoma induced by compound factors in rats显示文摘In China,the morbidity of alcoholic liver diseases (ALD)is increasing,which is the second cause of liver diseases after viral hepatitis.An epidemiological survey of 99 subjects in Kweichow Maotai Distillery who have drunk a lot of Maotai liquor for a long time showed that fatty livers were predominate and the incidence of hepatic fibrosis and cirrhosis was relatively low.1 Now,we carried out an animal experiment to investigate the mutation of P53 gene in liver tissues of primary hepatocellular carcinoma (HCC) induced by compound factors. | Zhao Xueke Cheng Mingliang Mu Mao Liu Junling He Chang Zhang Quan | 2014 | Chinese Medical Journal2014，，2： | 1 |
| 18 | Distribution of Bacterial Communities in Petroleum-Contaminated Soils from the Dagang Oilfield, China显示文摘Diversity in bacterial communities was investigated along a petroleum hydrocarbon content gradient(0-0.4043 g/g)in surface(5-10 cm)and subsurface(35-40 cm)petroleum-contaminated soil samples from the Dagang Oilfield,China.Using 16S rRNA Illumina high-throughput sequencing technology and several statistical methods,the bacterial diversity of the soil was studied.Subsequently,the environmental parameters were measured to analyze its relationship with the community variation.Nonmetric multidimensional scaling and analysis of similarities indicated a significant difference in the structure of the bacterial community between the nonpetroleum-contaminated surface and subsurface soils,but no differences were observed in different depths of petroleum-contaminated soil.Meanwhile,many significant correlations were obtained between diversity in soil bacterial community and physicochemical properties.Total petroleum hydrocarbon,total organic carbon,and total nitrogen were the three important factors that had the greatest impacts on the bacterial community distribution in the long-term petroleum-contaminated soils.Our research has provided references for the bacterial community distribution along a petroleum gradient in both surface and subsurface petroleum-contaminated soils of oilfield areas. | Xueke Feng Zhen Liu Xiaoqiang Jia Wenyu Lu | 2020 | Transactions of Tianjin University2020，26，1： | 1 |
| 19 | Ac_(3)6deoGlcNAz could selectively label O-GlcNAc modified proteins with minimal S-glyco-modification显示文摘A novel metabolic chemical reporter of Ac_(3)6deo Glc NAz was developed and confirmed as an effective probe for O-Glc NAc modification. Ac_(3)6deo Glc NAz labeling predominantly occurs in intracellular OGlc NAcylated proteins rather than cell-surface glycoproteins. Of note, it could reduce the artificial S-glycomodification compared to Ac_(4)Gal NAz and Ac_(4)Glc NAz. This new reporter allows to be widely used in the field of proteomic identification of O-Glc NAcylation. | Lu Zheng Wei Cao Biao Dou Xueke Zeng Mingya Cao Jiajia Wang Xia Li | 2023 | Chinese Chemical Letters2023，34，2： | 1 |
| 20 | Studies on the relative reactivity of three hydroxyl groups in aconitine 显示文摘 | XueKe She Xi-Xian Jian Dong-Lin Chen Qiao-Hong Chen and Feng- Pe ng Wang | 2012 | Journal of Asian Natural Products Research2012，14，： | 1 |
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