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| 1 | Honokiol induces apoptosis through p53-independent pathway in human colorectal cell line RKO显示文摘AIM: To investigate the signal pathway of honokiol-induced apoptosis on human colorectal carcinoma RKO cells and to evaluate whether p53 and p53-related genes were involved in honokiol-treated RKO cells.METHODS: Cell cycle distribution and subdiploid peak were analyzed with a flow cytometer and DNA fragment with electrophoresis on agarose gels. Transcriptional level of Bax, Bcl-2, Bid and Bcl-xl was accessed by RT-PCR.Western blotting was used to measure p53 protein expression and other factors related to apoptosis.Proliferation inhibition of two cell lines (RKO, SW480) with high expression of p53 and one cell line with p53 negative expression (LS180) was monitored by MTr assay.RESULTS: Honokiol induced RKO cell apoptosis in a dosedependent manner. The mRNA expression level and proteinlevel of Bid were up-regulated while that of Bcl-xl wasdown-regulated, but no changes in Bax and Bcl-2 were observed. Western blotting showed p53 expression had no remarkable changes in honokiol-induced RKO cell apoptosis. LS180 cells treated with honokiol exhibited apparent growth inhibition like RKO ceils and Sw480 ceils.CONCLUSION: Honokiol can induce RKO cells apoptosis through activating caspase cascade by p53-indepenent pathway. | TaoWang FeiChen ZheChen Yi-FengWut Xiao-LiXu ShuZheng XunHu | 2004 | World Journal of Gastroenterology2004，10，15： | 27 |
| 2 | Honokiol:A potent chemotherapy candidate for human colorectal carcinoma显示文摘AIM: To investigate the anticancer activity of Honokiol on RKO,a human colorectal carcinoma cell line in vitro and in vivo,and to evaluate its possible use in clinic.METHODS: In vitro anticancer activity of honokiol was demonstrated by its induction of apoptosis in tumor cells.We analyzed cell proliferation with MTT assay, cell cycle with flow cytosmeter, DNA fragment with electrophoresis on agarose gels. To test the mechanism of honokiol-induced apoptosis, Westem blotting was used to investigate the factors involved in this process. The pharmacokinetics study of honokiol was tested by high phase liquid chromatography.In in vivo study, Balb/c nude mice were incubated with RKO cells. Honokiol was injected intraperitoneally every other day into tumor bearing Balb/c nude mice.RESULTS: Our results showed that honokiol induced apoptosis of RKO cells in a time- and dose-dependent manner. At 5-10 ug/mL for 48 h, honokiol induced apoptosis through activating Caspase cascades. Pharmacokinetics study demonstrated that, honokiol could be absorbed quickly by intraperitoneal injection, and maintained in plasma for more than 10 h. In nude mice bearing RKO-incubated tumor, honokiol displayed anticancer activity by inhibiting tumor growth and prolonging the lifespan of tumor bearing mice.CONCLUSION: With its few toxicity to normal cells and potent anticancer activity in vitroand in vivo, honokiol might be a potential chemotherapy candidate in treating human colorectal carcinoma. | FeiChen TaoWang Yi-FengWu YingGu Xiao-LiXu ShuZheng XunHu | 2004 | World Journal of Gastroenterology2004，10，23： | 23 |
| 3 | Properties and biocompatibility of chitosan films modified by blending with PEG显示文摘 | Zhang Miao Li Xunhu Gong Yandao Zhao Nanming Zhang Xiufang | | 0，，： | 1 |
| 4 | Low temperature co-fired ceramic substrate显示文摘 | Dai Steve Xunhu Huang Rongfong David L | 2002 | Jam Ce- ram Soc2002，85，4： | 1 |
| 5 | Service-based Reliability Analysis of Integrated Electricity-heat Systems Considering Thermal Dynamics显示文摘The essential task of integrated electricity-heat systems(IEHSs)is to provide customers with reliable electric and heating services.From the perspective of customers,it is reasonable to analyze the reliabilities of IEHSs based on the ability to provide energy services with a reasonable assurance of continuity and quality,which are termed as service-based reliabilities.Due to the thermal inertia existing in IEHSs,the heating service performances can present slow dynamic characteristics,which has a great impact on the service satisfaction of customers.The neglect of such thermal dynamics will bring about inaccurate service-based reliability measurement,which can lead to the inefficient dispatch decisions of system operators.Therefore,it is necessary to provide a tool which can analyze the servicebased reliabilities of IEHSs considering the impacts of thermal dynamics.This paper firstly models the energy service performance of IEHSs in contingency states.Specifically,the nodal energy supplies are obtained from the optimal power and heat flow model under both variable hydraulic and thermal conditions,in which the transmission-side thermal dynamics are formulated.On this basis,the energy service performances for customers are further determined with the formulation of demandside thermal dynamics.Moreover,a service-based reliability analysis framework for the IEHSs is proposed utilizing the timesequential Monte Carlo simulation(TSMCS)technique with the embedded decomposition algorithm.Furthermore,the indices for quantifying service-based reliabilities are defined based on the traditional reliability indices,where dynamic service performances and service satisfactions of customers are both considered.Numerical simulations are carried out with a test system to validate the effectiveness of the proposed framework. | Xunhu Yin Minglei Bao Yi Ding Chengjin Ye Peng Wang Lalit Goel | 2023 | Journal of Modern Power Systems and Clean Energy2023，11，4： | 0 |
| 6 | Oxidation mechanism of three Fe-Al alloys with and without addition of 0.1 at% yttrium at 800℃显示文摘The isothermal oxidation behavior of Fe-10 Al,Fe-15 Al,Fe-20 Al alloys with and without the addition of0.1 at% Y was studied at 800℃ under 1×105 Pa of flowing pure O2 for 24 h.The oxidation of three Fe-Al alloys can be divided into transient state and steady state oxidation stages.The oxidation of each stage is approximately in accordance with the parabolic law.The addition of 0.1 at% Y changes the oxidation behavior obviously and leads to a significant increase of the weight gain of Fe-10 Al and Fe-15 Al.The scale grown on Fe-10 Al is much thicker and more complicated than that grown on Fe-20 Al,which is composed of an exclusive thin layer of Al2 O3 protective film.Due to the formation of a large number of nodules,the scales grown on Fe-15 AI cannot provide full protection for the alloy.Scale micro structure of the three Fe-Al-0.1 Y alloys is similar to their corresponding Fe-Al alloys.However,nodules with very small size still appear on the surface of Fe-20 Al-0.1 Y alloy.The critical Al concentration to form an exclusive Al2 O3 protective layer for Fe-Al binary alloy is on the borderline between 15 at%-20 at%.For Fe-Al-0.1 Y alloy,the presence of 20 at% Al is not enough to inhibit the growth of nodules. | Xunhu Xu Hua Wei Junhuai Xiang Ling Wang Mingfeng Liu Honghua Zhang Dandan Men Jiangshan An | 2020 | Journal of Rare Earths2020，38，10： | 0 |
| 7 | Targeting TRPV1-mediated autophagy attenuates nitrogen mustard-induced dermal toxicity显示文摘Nitrogen mustard(NM)causes severe vesicating skin injury,which lacks effective targeted therapies.The major limitation is that the specific mechanism of NM-induced skin injury is not well understood.Recently,autophagy has been found to play important roles in physical and chemical exposure-caused cutaneous injuries.However,whether autophagy contributes to NM-induced dermal toxicity is unclear.Herein,we initially confrmed that NM dose-dependently caused cell death and induced autophagy in keratinocytes.Suppression of autophagy by 3-methyladenine,chloroquine,and baflomycin A1 or ATG5 siRNA attenuated NM-induced keratinocyte cell death.Furthermore,NM increased transient receptor potential vanilloid 1(TRPV1)expression,intracellular Ca2+content,and the activities of Car+/calmodulin-dependent kinase kinaseβ(CaMKKB),AMP-activated protein kinase(AMPK),unc-51-like kinase 1(ULK1),and mammalian target of rapamycin(mTOR).NM-induced autophagy in keratinocytes was abolished by treatment with inhibitors of TRPV1(capsazepine),CaMKKβ(STO-609),AMPK(compound C),and ULK1(SBI-0206965)as well as TRPV1,CaMKKB,and AMPK siRNA transfection.In addition,an mTOR inhibitor(rapamycin)had no signifcant effect on NM-stimulated autophagy or cell death of keratinocytes.Finally,the results of the in vivo experiment in NM-treated skin tissues were consistent with the findings of the in vitro experiment.In conclusion,NM-caused dermal toxicity by overactivating autophagy partially through the activation of TRPV1-Ca2+-CaMKKB-AMPK-ULK1 signaling pathway.These results suggest that blocking TRPV1-dependent autophagy could be a potential treatment strategy for NM-caused cutaneous injury. | Mingliang Chen Xunhu Dong Haoyue Deng Feng Ye Yuanpeng Zhao Jin Cheng Guorong Dan Jiqing Zhao Yan Sai Xiuwu Bian Zhongmin Zou | 2021 | Signal Transduction and Targeted Therapy2021，6，2： | 0 |
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