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| 1 | Current Trends of the Prevalence of Childhood Asthma in Three Chinese Cities: A Multicenter Epidemiological Survey显示文摘Objective To analyze the prevalence of asthma and asthma related symptoms among children aged 0-14 years in three Chinese cities and to obtain a crude estimation of the trend of childhood asthma prevalence in China. Methods A cross-sectional, population-based survey of prevalence of asthma was conducted in children aged from 0 to 14 years in 3 major cities of China (Beijing, Chongqing, and Guangzhou) with different geographic locations. All the subjects were randomly selected by a multi-stage sampling method. Three to five schools and kindergartens in 2 urban districts in each city were randomly selected for the survey, and a validated questionnaire that included the core questions of the International Study of Asthma and Allergies in Childhood, Phase Ⅲ questionnaire and several additional questions were used. All questionnaires were completed by parents or guardians of the selected children. Children whose parents responded affirmatively to the question' Has your child ever been diagnosed as asthma by a doctor' were recognized as victims of asthma. Results The prevalence of asthma in Beijing, Chongqing, and Guangzhou was 3.15%, 7.45%, and 2.09%, respectively. These values were significantly higher than those obtained 10 years ago in the national epidemiological survey in 2000 which used the same method of investigation and the same diagnotic criteria (χ2 =3.938, P=0.047; χ2 =73.506, P≤0.001; χ2 =11.956, P=0.001, in each city). Of the asthmatic children 57.21%, 69.91%, and 60.00% had their first attack before the age of 3 in Beijing, Chongqing, and Guangzhou, respectively. Wheezing was the primary clinical manifestation for all asthmatic children , followed by persistent cough and repeated respiratory infections. Both the prevalence of asthma and asthma-related symptoms were statistically higher in males than in females. Conclusion The prevalence of childhood asthma is statistically higher than that 10 years ago in the three Chinese cities. | JUAN BAI JiNG ZHAO KUN-LING SHEN LI XIANG AI-HUAN CHEN SUI HUANG YING HUANG JIAN-SHENG WANG RONG-WEI YE | 2010 | Biomedical and Environmental Sciences2010，23，6： | 37 |
| 2 | Recurrent gain-of-function USP8 mutations in Cushing's disease显示文摘库欣的疾病,引起过量皮质醇生产的也已知的同样促肾上腺皮质的荷尔蒙(ACTH )-secreting 垂体腺瘤(舞步) ,占多达 85%corticotrophin 依赖的库欣的症候群盒子。然而,在这疾病的基因改变是不清楚的。这里,我们执行了从 12 秘密 ACTH 舞步导出并且匹配血样品的 DNA 定序的 whole-exome,它在候选人基因揭示了体的变化的三种类型, USP8 (编码 ubiquitin 特定的朊酶 8 ) ,专门在 exon 14 在 12 秘密 ACTH 舞步中的 8 个。我们进一步评估了体的 USP8 变化在另外由定序的 Sanger 的 258 舞步。进一步定序指向在 108 秘密 ACTH 舞步(62.04%) 中的 67 个识别了 USP8 变体的 17 种类型的一个总数。然而,任何一个都没在舞步的另外的类型这些变化被检测(n = 150 ) 。这些变化在 USP8 的 14-3-3 绑定主题以内聚集并且破坏在 USP8 和 14-3-3 蛋白质之间的相互作用,导致保护 EGFR 免受 lysosomal 降级的伤害的一个提高的能力。因此,有 EGFR 表示,提高的 EGFR 蛋白质丰富和 mRNA 表示的更高的发生 POMC 铺平的变异的 USP8 显示的舞步,它编码 ACTH 的先锋。有变异的 USP8 的舞步在尺寸是显著地更小的并且比野类型的舞步有更高的 ACTH 生产。在里面通过手术 resected 主要变异 USP8 的肿瘤房间, USP8 击倒或堵住的 EGFR 有效地稀释 ACTH 分泌物。一起拿,体的 gain-of-function USP8 变化是普通的并且在库欣的疾病贡献 ACTH 生产过剩。USP8 或 EGFR 的抑制为对待变异 USP8 的 corticotrophin 腺瘤是有希望的。我们的学习在库欣的疾病加亮潜在地功能的变异的基因并且提供卓见进这疾病的治疗学。 | Zeng-Yi Ma Zhi-Jian Song Jian-Hua Chen Yong-Fei Wang Shi-Qi Li Liang-Fu Zhou Ying Mao Yi-Ming Li Rong-Gui Hu Zhao-Yun Zhang Hong-Ying Ye Ming Shen Xue-Fei Shou Zhi-Qiang Li Hong Peng Qing-Zhong Wang Dai-Zhan Zhou Xiao-Lan Qin Jue Ji Jie Zheng Hong Chen Yin Wang Dao-Ying Geng Wei-Jun Tang Chao-Wei Fu Zhi-Feng Shi Yi-Chao Zhang Zhao Ye Wen-Qiang He Qi-Lin Zhang Qi-Sheng Tang Rong Xie Jia-Wei Shen Zu-Jia Wen Juan Zhou Tao Wang Shan Huang Hui-Jia Qiu Ni-Dan Qiao Yi Zhang Li Pan Wei-Min Bao Ying-Chao Liu Chuan-Xin Huang Yong-Yong Shi Yao Zhao | 2015 | Cell Research2015，25，3： | 16 |
| 3 | Polysaccharides from Tricholoma matsutake and Lentinus edodes enhance 5-fluorouracil-mediated H22 cell growth inhibition显示文摘OBJECTIVE: Few studies have investigated the effects produced by combinations of polysaccharides and chemotherapeutic drugs in cancer treatment.We hypothesized that a combinationo fpolysaccha rides(COP) from Lentinus edodes and Tricholoma matsutake would improve the efficacy of 5-fluorouracil(5-FU)-mediated inhibition of H22 cell growth.METHODS: Mice were injected H22 cells and then treated with either 5-FU, polysaccharides from Tricholoma matsutake(PTM), polysaccharides from Lentinus edodes(PL), PTM+PL, 5-FU+PTM, 5-FU+PL, or 5-FU+COP. The tumor weight and volume,and splenic CD4 + and CD8 + T cell frequencies,were determined. Additionally, splenic natural killer(NK) cell and cytotoxic Tlymphocyte (CTL) activities were assessed and the serum levels of tumor necrosis factor-α(TNF-α),Interleukin-2(IL-2),and Interferon-γ (IFN-γ) were measured. 5-FU, PL, PTM, PTM+PL, 5-FU+PL, and 5-FU+PTM groups, mice treated with 5-FU+COP showed:(a)significantly reduced tumor weight and volume(P<0.05);(b) significantly higher serum levels ofTNF-α,IL-2, and IFN-γ(P<0.05);(c) significantly increased CD4+andCD8+Tcell frequencies in the spleen(P<0.05); and(d) significantly increased splenic NK cell and CTL activities(P<0.05). The tumor weight and volume in mice treated with 5-FU+PL or 5-FU+PTM were significantly reduced compared with mice treated with 5-FU alone(P<0.05). Serum levels of TNF-α, IL-2, and IFN-γ, frequencies of CD4 + and CD8+T cells in the spleen, and splenic NK and CTL activities were also significantly increased in mice treated with 5-FU+PL or 5-FU+PTM compared with mice treated with 5-FU alone (P<0.05).CONCLUSION: Polysaccharides from Lentinus edodes and Tricholoma matsutake could enhance the efficacy of 5-FU-mediated H22 cell growth inhibition. | Ming Ren Lingyan Ye Xiaoshi Hao Zhixing Ren Shuping Ren Kun Xu Juan Li | 2014 | Journal of Traditional Chinese Medicine2014，34，3： | 15 |
| 4 | Safety,tolerability,and pharmacokinetics of VV116,an oral nucleoside analog against SARS-CoV-2,in Chinese healthy subjects显示文摘VV116(JT001)is an oral drug candidate of nucleoside analog against SARS-CoV-2.The purpose of the three phase I studies was to evaluate the safety,tolerability,and pharmacokinetics of single and multiple ascending oral doses of VV116 in healthy subjects,as well as the effect of food on the pharmacokinetics and safety of VV116.Three studies were launched sequentially:Study 1(single ascending-dose study,SAD),Study 2(multiple ascending-dose study,MAD),and Study 3(food-effect study,FE).A total of 86 healthy subjects were enrolled in the studies.VV116 tablets or placebo were administered per protocol requirements.Blood samples were collected at the scheduled time points for pharmacokinetic analysis.116-N1,the metabolite of VV116,was detected in plasma and calculated for the PK parameters.In SAD,AUC and Cmax increased in an approximately dose-proportional manner in the dose range of 25–800 mg.T_(1/2)was within 4.80–6.95 h.In MAD,the accumulation ratio for Cmax and AUC indicated a slight accumulation upon repeated dosing of VV116.In FE,the standard meal had no effect on Cmax and AUC of VV116.No serious adverse event occurred in the studies,and no subject withdrew from the studies due to adverse events.Thus,VV116 exhibited satisfactory safety and tolerability in healthy subjects,which supports the continued investigation of VV116 in patients with COVID-19. | Hong-jie Qian Yu Wang Meng-qi Zhang Yuan-chao Xie Qing-qing Wu Li-yu Liang Ye Cao Hua-qing Duan Guang-hui Tian Juan Ma Zhuo-bing Zhang Ning Li Jing-ying Jia Jing Zhang Haji Akber Aisa Jing-shan Shen Chen Yu Hua-liang Jiang Wen-hong Zhang Zhen Wang Gang-yi Liu | 2022 | Acta Pharmacologica Sinica2022，43，12： | 13 |
| 5 | A human circulating immune cell landscape in aging and COVID-19显示文摘Age-associated changes in immune cells have been linked to an increased risk for infection.However,a global and detailed characterization of the changes that human circulating immune cells undergo with age is lacking.Here,we combined scRNA-seq,mass cytometry and sCATAC-seq to compare immune cell types in peripheral blood collected from young and old subjects and patients with COVID-19.We found that the immune cell landscape was reprogrammed with age and was characterized by T cell polarization from naive and memory cells to effector,cytotoxic,exhausted and reg-ulatory cells,along with increased late natural killer cells,age-associated B cells,inflammatory monocytes and age-associated dendritic cells.In addition,the expression of genes,which were implicated in coron-avirus susceptibility,was upregulated in a cell subtype-specific manner with age.Notably,COVID-19 promoted age-induced immune cell polarization and gene expression related to inflammation and cellular senes-cence.Therefore,these findings suggest that a dysreg-ulated immune system and increased gene expression associated with SARS-CoV-2 susceptibility may at least partially account for COVID-19 vulnerability in the elderly. | Yingfeng Zheng Xiuxing Liu Wenqing Le Lihui Xie He Li Wen Wen Si Wang Shuai Ma Zhaohao Huang Jinguo Ye Wen Shi Yanxia Ye Zunpeng Liu Moshi Song Weiqi Zhang Jing-Dong J.Han Juan Carlos lzpisua Belmonte Chuanle Xiao Jing Qu Hongyang Wang Guang-Hui Liu Wenru Su | 2020 | Protein & Cell2020，11，10： | 10 |
| 6 | COMPUTATIONAL RESEARCH ON WAVE MAKING OF MOVING WIGLEY HULL IN TIME DOMAIN显示文摘Based on Green’s theorem, a time domain numerical model was constructed to simulate wave making phenomenon caused by a moving ship. In this article, the Rankine sources and dipoles were placed on boundary surfaces (i.e., the ship surface and free surface), and a time-stepping scheme was employed. Its unique characteristic is that steady state can be realized from initial value by employing the time-stepping scheme and unsteady free surface conditions. In time domain, if the results of unsteady flow problem tend to data stabilization after many time steps of computation, they could be regarded as the data of steady ones. This model could be employed to steady or unsteady problems. Theoretical reasoning and computational process of this method was described in detail. The linear and nonlinear boundary conditions on body surface were studied, and the relative means to realize these boundary conditions in iterative computation were also discussed. Some proper parameters about the model of the Wigley hull were determined by many numerical tests, and their influences on wave making resistance and wave pattern were discussed. According to the comparison between numerical results and data available in relative references, the method used in this work is proven to be a reliable method in time domain. And the lattice reorganization in every time step computation is a feasible numerical approach. | YANG Xiang-hui YE Heng-kui FENG Da-kui LIU Juan | 2008 | Journal of Hydrodynamics2008，20，4： | 10 |
| 7 | Blood Pressure Associated with Arsenic Methylation and Arsenic Metabolism Caused by Chronic Exposure to Arsenic in Tube Well Water显示文摘Objective The effects of arsenic exposure from drinking water, arsenic metabolism, and arsenic methylation on blood pressure(BP) were observed in this study. Methods The BP and arsenic species of 560 participants were determined. Logistic regression analysis was applied to estimate the odds ratios of BP associated with arsenic metabolites and arsenic methylation capability. Results BP was positively associated with cumulative arsenic exposure(CAE). Subjects with abnormal diastolic blood pressure(DBP), systolic blood pressure(SBP), and pulse pressure(PP) usually had higher urinary i As(inorganic arsenic), MMA(monomethylated arsenic), DMA(dimethylated arsenic), and TAs(total arsenic) than subjects with normal DBP, SBP, and PP. The i As%, MMA%, and DMA% differed slightly between subjects with abnormal BP and those with normal BP. The PMI and SMI were slightly higher in subjects with abnormal PP than in those with normal PP. Conclusion Our findings suggest that higher CAE may elevate BP. Males may have a higher risk of abnormal DBP, whereas females have a higher risk of abnormal SBP and PP. Higher urinary i As may increase the risk of abnormal BP. Lower PMI may elevate the BP. However, higher SMI may increase the DBP and SBP, and lower SMI may elevate the PP. | WEI Bing Gan YE Bi Xiong YU Jiang Ping YANG Lin Sheng LI Hai Rong XIA Ya Juan WU Ke Gong | 2017 | Biomedical and Environmental Sciences2017，30，5： | 9 |
| 8 | Kinesin-4 Functions in Vesicular Transport on Cortical Microtubules and Regulates Cell Wall Mechanics during Cell Elongation in Plants显示文摘在植物,各向异性的房间扩大取决于作为轨道服务哪个大分子和泡被未知身份的马达 kinesins 搬运的外皮的微导管。我们使用了经历了柔韧的延伸发现涉及房间延伸并且发现 Gh KINESIN-4A 的 kinesins 的纤维富有地表示了的棉花(Gossypium hirsutum ) 。马达被 immunofluorescence 在与外皮的微导管被联系的像泡的结构上检测。在 Arabidopsis thaliana,在 KINESIN-4A/FRA1 的 orthologous 马达,以前在纤维细胞在第二等的生长期间在纤维素免职含有,被实时房间的成像在表示荧光灯地标注的功能的蛋白质的细胞检验。马达装饰了与 0.89m/min 的平均速度沿着外皮的微导管展出一个线性运动的像泡的粒子,它与连接到纤维素生合成的那些显著地不同。我们也在 KINESIN-4A/FRA1 发现了那并且在细胞墙力学,细胞延伸,和各种各样的植物、繁殖的器官的轴的生长在 KINESIN-4C 玩联系了冗余的角色,,损失在极大地提高的 KINESIN-4C,缺点在 KINESIN-4A/FRA1 地点由一个空变化引起了。双异种显示了房间墙在快速的房间延伸的正常阶段弄软的缺乏。而且,提高了包含 arabinose 的免职糖类在 kinesin-4 异种被检测。我们的调查结果建立了在在 flowering 植物沿着外皮的微导管和房间墙力学和房间延伸包含非有纤维质的部件的货物的 Kinesin-4-based 运输之间的一个连接。 | Zhaosheng Kong Motohide Ioki Siobhan Braybrook Shundai Li Zheng-Hua Ye Yuh-Ru Julie Lee Takashi Hotta Anny Chang Juan Tian Guangda Wang Bo Liu | 2015 | Molecular Plant2015，8，7： | 8 |
| 9 | Two Lycopene β-Cyclases Genes from Sweet Orange (Citrus sinensis L. Osbeck) Encode Enzymes With Different Functional Efficiency During the Conversion of Lycopene-to-Provitamin A显示文摘Citrus fruits are rich in carotenoids.In the carotenoid biosynthetic pathway,lycopene β-cyclase(LCYb,EC:1.14.-.-) is a key regulatory enzyme in the catalysis of lycopene to β-carotene,an important dietary precursor of vitamin A for human nutrition.Two closely related lycopene β-cyclase cDNAs,designated CsLCYb1 and CsLCYb2,were isolated from the pulp of orange fruits(Citrus sinensis).The expression level of CsLCYb genes is lower in the flavedo and juice sacs of a lycopeneaccumulating genotype Cara Cara than that in common genotype Washington,and this might be correlated with lycopene accumulation in Cara Cara fruit.The CsLCYb1 efficiently converted lycopene into the bicyclic β-carotene in an Escherichia coli expression system,but the CsLCYb2 exhibited a lower enzyme activity and converted lycopene into the β-carotene and the monocyclic γ-carotene.In tomato transformation studies,expression of CsLCYb1 under the control of the cauliflower mosaic virus(CaMV) 35S constitutive promoter resulted in a virtually complete conversion of lycopene into β-carotene,and the ripe fruits displayed a bright orange colour.However,the CsLCYb2 transgenic tomato plants did not show an altered fruit colour during development and maturation.In fruits of the CsLCYb1 transgenic plants,most of the lycopene was converted into β-carotene with provitamin A levels reaching about 700 μg g-1DW.Unexpectedly,most transgenic tomatoes showed a reduction in total carotenoid accumulation,and this is consistent with the decrease in expression of endogenous carotenogenic genes in transgenic fruits.Collectively,these results suggested that the cloned CsLCYb1 and CsLCYb2 genes encoded two functional lycopene β-cyclases with different catalytic efficiency,and they may have potential for metabolite engineering toward altering pigmentation and enhancing nutritional value of food crops. | ZHANG Jian-cheng ZHOU Wen-jing XU Qiang TAO Neng-guo YE Jun-li GUO Fei XU Juan DENG Xiu-xin | 2013 | Journal of Integrative Agriculture2013，12，10： | 8 |
| 10 | Primary evidence for involvement of IP_(3) in heat-shock signal transduction in Arabidopsis显示文摘热吃惊(HS ) 表明的在 transducing 的肌醇 1,4,5-trisphosphate (IP (3 )) 的角色在 Arabidopsis 被检验。整个植物的 IP (3 ) 水平在 37 度 C 在 HS 的 1 min 以内增加了。在 HS 的 3 min 以后, IP (3 ) 水平到达了最大值 2.5 褶层增加。用有 AtHsp18.2 promoter-beta-glucuronidase ( GUS )的转基因的 Arabidopsis 植物熔化基因, GUS 活动的水平在 non-HS 和 HS 温度由将关入笼中的 IP ( 3 )的增加是起来调整的并且由 phospholipase C ( PLC )禁止者是下面调整的,这被发现{1-[6-(( 17beta-3-Methoxyestra-1,3,5 ( 10 ) -trien-17-yl)amino)hexyl]-2,5-pyrro lidinedione }( U-73122 )。细胞内部免费的钙离子集中([Ca (2+)](i)) 在表示 apoaequorin 的暂停有教养的 Arabidopsis 房间在 37 度 C 在 HS 期间增加了。有 U-73122 的处理阻止了增加[Ca (2+)](i) 到某程度。上面的结果在更高的植物在 HS 信号 transduction 为 IP (3 ) 的可能的参与提供了主要证据。 | Hong Tao Liu Fei Gao Shu Juan Cui Jin Long Han Da Ye Sun Ren Gang Zhou | 2006 | Cell Research2006，16，4： | 7 |
| 11 | 15-deoxy-△12,14-prostaglandin J2 ameliorates endotoxin-induced acute lung injury in rats显示文摘 | Geng Zhilong Zhu Wankun Wang Huiwen Chen Ye Liang Juan Liu Dong | 2014 | Chinese Medical Journal2014，，5： | 7 |
| 12 | Increased circulating circular RNA103516 is a novel biomarker for inflammatory bowel disease in adult patients显示文摘BACKGROUND Increasing evidence demonstrates that by acting as microRNA sponges modulating gene expression at the transcriptional or post-transcriptional level,circular RNAs(circRNAs)participate in the pathogenesis of a variety of diseases and are considered ideal biomarkers of human disease.AIM To examine the expression of circRNA_103516 in inflammatory bowel disease(IBD)and its associations with clinical phenotypes and inflammatory cytokines.METHODS Peripheral blood mononuclear cells(PBMCs)were obtained from patients with IBD,healthy controls(HCs),and patient controls(PCs).Expression of circRNA_103516 and hsa-miR-19b-1-5p was assessed by quantitative reverse transcription-polymerase chain reaction.Crohn's disease activity index(CDAI),Mayo score,C-reactive protein(CRP)level,and erythrocyte sedimentation rate(ESR)were measured.To assess the inflammatory cytokines tumour necrosis factorα(TNF-α),interferon-γ(IFN-γ),and interleukin-10(IL-10),blood samples were analysed by flow cytometry.RESULTS Ninety Crohn’s disease(CD)and 90 ulcerative colitis(UC)patients,80 HCs,and 35 PCs were included in the study.CircRNA_103516 was upregulated in CD and UC patients compared with HCs and PCs(P<0.05).The area under the curve of circRNA_103516 for diagnosing CD and UC was 0.790 and 0.687,respectively.In addition,circRNA_103516 levels were increased in active CD and UC compared with remittent groups(P=0.027,P=0.045).Furthermore,in CD,circRNA_103516 correlated positively with CDAI(P<0.001),CRP(P<0.001),ESR(P<0.001),TNFα(P<0.001),and IFN-γ(P<0.001)and negatively correlated with IL-10(P=0.006).In UC patients,circRNA_103516 correlated with Mayo score(P<0.001),CRP(P<0.001),ESR(P<0.001),TNFα(P<0.001),IFN-γ(P=0.011),and IL-10(P=0.002).Additionally,circRNA_103516 correlated positively with stricturing(P=0.018)and penetrating(P=0.031)behaviour.Moreover,hsamiR-19b-1-5p correlated negatively with circRNA_103516 in CD.CONCLUSION CircRNA_103516 levels in PBMCs can be considered an ideal candidate biomarker for diagnosing IBD.Dysregulation of circRNA_103516 may participate in the molecular mechanism of IBD through hsa-miR-19b-1-5p sponging. | Yu-Lan Ye Juan Yin Tong Hu Li-Ping Zhang Long-Yun Wu Zhi Pang | 2019 | World Journal of Gastroenterology2019，25，41： | 6 |
| 13 | Clinical applications of exosome membrane proteins显示文摘Extracellular vesicles(EVs)are small membranous particles that can mediate cell-to-cell communication and which are divided into at least three categories according to their subcellular origin and size:exosomes,microvesicles,and apoptotic bodies.Exosomes are the smallest(30–150 nm)of these EVs,and play an important role in EV-mediated cell-to-cell interactions,by transferring proteins,nucleic acids and,lipids from their parental cells to adjacent or distant cells to alter their phenotypes.Most exosome studies in the past two decades have focused on their nucleic acid composition and their transfer ofmRNAs and microRNAs to neighboring cells.However,exosomes also carry specific membrane proteins that can identify the physiological and pathological states of their parental cells or indicate their preferential target cells or tissues.Exosome membrane protein expression can also be directly employed or modified to allow exosomes to serve as drug delivery systems and therapeutic platforms,including in targeted therapy approaches.This review will briefly summarize information on exosome membrane proteins components and their role in exosome–cell interactions,including proteins associated with specific cell-interactions and diseases,and the potential for using exosome membrane proteins in therapeutic targeting approaches. | Qian Hu Hang Su Juan Li Christopher Lyon Wenfu Tang Meihua Wan Tony Ye Hu | 2020 | Precision Clinical Medicine2020，3，1： | 6 |
| 14 | Epidemiology of urticaria in China:a population-based study显示文摘Background:Urticaria is a common skin disease characterized by episodes of wheals,and it has a negative effect on patients’quality of life.Large-scale population-based epidemiological studies of urticaria are scarce in China.The aim of this survey was to determine the prevalence,clinical forms,and risk factors of urticaria in the Chinese population.Methods:This survey was conducted in 35 cities from 31 provinces,autonomous regions,and municipalities of China.Two to three communities in each city were selected in this investigation.Participants completed questionnaires and received dermatological examinations.We analyzed the prevalence,clinical forms,and risk factors of urticaria.Results:In total,44,875 questionnaires were distributed and 41,041 valid questionnaires were collected(17,563 male and 23,478 female participants).The lifetime prevalence of urticaria was 7.30%,with 8.26%in female and 6.34%in male individuals(P<0.05).The point prevalence of urticaria was 0.75%,with 0.79%in female and 0.71%in male individuals(P<0.05).Concomitant angioedema was found in 6.16%of patients.Adults had a higher prevalence of urticaria than adolescents and children.Living in urban areas,exposure to pollutants,an anxious or depressed psychological status,a personal and family history of allergy,thyroid diseases,and Helicobacter pylori infection were associated with a higher prevalence of urticaria.Smoking was correlated with a reduced risk of urticaria.Conclusion:This study demonstrated that the lifetime prevalence of urticaria was 7.30%and the point prevalence was 0.75%in the Chinese population;women had a higher prevalence of urticaria than men.Various factors were correlated with urticaria. | Jiaqing Li Dandan Mao Shuoshuo Liu Ping Liu Jing Tian Chenhong Xue Xiaojing Liu Ruiqun Qi Bingxue Bai Jianjun Nie Siqi Ye Yu Wang Yuye Li Qing Sun Juan Tao Shuping Guo Hong Fang Jianqin Wang Qiri Mu Quanzhong Liu Yan Ding Jianzhong Zhang | 2022 | Chinese Medical Journal2022，，11： | 6 |
| 15 | Evaluation of the analgesic effects of ammoxetine, a novel potent serotonin and norepinephrine reuptake inhibitor显示文摘瞄准:选择血清素(5-HT ) 和新肾上素(NE ) 举起禁止者(SNRI ) 通常为神经病的疼痛和 fibromyalgia 的治疗被使用。Ammoxetine ((±)-3-(benzo[d][1,3 ] dioxol-4-yloxy )-N-methyl-3-(thiophen-2-yl)propan-1-amine) 作为新奇有势力 SNRI 被识别了。在这研究,我们在疼痛的不同动物模型评估了 ammoxetine 的尖锐止痛性质,并且在它的止痛 actions.Methods 检验了 monoamines 的参与:ammoxetine 的止痛效果是用在老鼠,臀部的神经损害(SNI ) 导致的神经病的疼痛,长期的收缩损害(CCI ) 和在老鼠的导致利血平的 fibromyalgia 疼痛的醋性的导致酸、导致福尔马林的疼痛的模型的 assayed。在 fibromyalgia 老鼠的大脑区域的 5-HT 和 NE 的内容用 HPLC-ECD 被测量。在所有实验, duloxetine 被用作积极控制 drug.Results:ammoxetine (0.625-10 mg/kg ) 或 duloxetine (2.5-40 mg/kg ) 的口头的管理 dose-dependently 减少了在老鼠的醋性的导致酸的挣扎和导致福尔马林的第一阶段和秒阶段爪舔时间的数字。ammoxetine (2.5-10 mg/kg ) 或 duloxetine (10 mg/kg ) 的口头的管理在 CCI 老鼠在 SNI 和 CCI 老鼠和热痛觉过敏减轻了机械 allodynia。在 CCI 老鼠的 ammoxetine 的 antiallodynic 效果被预告的处理与 para-chlorophenylalanine 甲基酉旨 hydrochloride 废除(PCPA,一个 5-HT 合成禁止者) 或 α -methyl-para-tyrosine methylester (AMPT,一个 catecholamine 合成禁止者) 。ammoxetine (30 mg/kg ) 或 duloxetine (50 mg/kg ) 的口头的管理显著地与导致利血平的 fibromyalgia 在老鼠稀释了触觉的 allodynia。在 fibromyalgia 老鼠, ammoxetine 的管理(10, 30 mg/kg ) 或 duloxetine (30, 50 mg/kg ) dose-dependently 增加了 5-HT 和 NE 的层次,并且减少在针的绳索,视下丘,丘脑和前额的 cortex.Conclusion 的 5-HT (5-HIAA/5-HT ) 的代谢物比率:Ammoxetine 有效地在动物模型减轻煽动性、连续、神经病、 fibromyalgia 相关的疼痛,它能在下降被归因于 5-HT 和 NE 的提高的 neurotransmission 禁止的系统。 | Ting-ting ZHANG Rui XUE Lei ZHU Juan LI Qiong-yin FAN Bo-hua ZHONG Yun-feng LI Cai-ying YE You-zhi ZHANG | 2016 | Acta Pharmacologica Sinica2016，37，9： | 6 |
| 16 | Identification and determination of the major constituents in traditional Chinese medicine Longdan Xiegan Pill by HPLC-DAD-ESI-MS显示文摘A novel and sensitive HPLC-UV method has been developed for the simultaneous determination of twelve major compounds in Longdan Xiegan Pill.The chemical profile of the twelve compounds,including geniposidic acid(1),geniposide(2),gentiopicroside(3),liquiritin(4),crocin(5),baicalin(6),wogonoside(7),baicalein(8),glycyrrhizic acid(9),wogonin(10),oroxylin A(11)and aristolochic acid A(12),was acquired using high-performance liquid chromatography-diode array detector coupled with an electrospray tandem mass spectrometer(HPLC-DAD-ESI-MS).The analysis was performed on a Dikma Platisil ODS C18 column(250 mm×4.6 mm,5 μm)with a gradient solvent system of acetonitrile-0.1% aqueous formic acid.The validation was carried out and the linearities(r>0.9996),repeatability(RSD<1.8%),intra-and inter-day precision(RSD<1.3%),and recoveries(ranging from 96.6% to 103.4%)were acceptable.The limits of detection(LOD)of these compounds ranged from 0.29 to 4.17 ng.Aristolochic acid A,which is the toxic ingredient,was not detected in all the batches of Longdan Xiegan Pill.Furthermore,hierarchical cluster analysis was used to evaluate the variation of the herbal prescription.The proposed method is simple,effective and suitable for the quality control of this traditional Chinese medicine(TCM). | Hui Liu Juan Su Xu Liang Xi Zhang Ya-Jun He Hai-Qiang Huan Ji Ye Wei-Dong Zhang | 2011 | Journal of Pharmaceutical Analysis2011，1，1： | 5 |
| 17 | Interpretation of the development of neoadjuvant therapy for gastric cancer based on the vicissitudes of the NCCN guidelines显示文摘Gastric cancer is one of the most common digestive system tumors in China,and locally advanced gastric cancer(LAGC)accounts for a high proportion of newly diagnosed cases.Although surgery is the main treatment for gastric cancer,surgical excision alone cannot achieve satisfactory outcomes in LAGC patients.Neoadjuvant therapy(NAT)has gradually become the standard treatment for patients with LAGC,and this treatment can not only achieve tumor downstaging and improve surgical rate and the R0 resection rate,but it also significantly improves the long-term prognosis of patients.Peri/preoperative neoadjuvant chemotherapy and preoperative chemoradiotherapy are both recommended according to a large number of studies,and the regimens have also been evolved in the past decades.Since the NCCN guidelines for gastric cancer are one of the most authoritative evidence-based guidelines worldwide,here,we demonstrate the development course and major breakthroughs of NAT for gastric cancer based on the vicissitudes of the NCCN guidelines from 2007 to 2019,and also discuss the future of NAT. | Xian-Ze Wang Zi-Yang Zeng Xin Ye Juan Sun Zi-Mu Zhang Wei-Ming Kang | 2020 | World Journal of Gastrointestinal Oncology2020，12，1： | 5 |
| 18 | Congenital Cystic Adenomatoid Malformation Volume Ratio in Prenatal Assessment of Prognosis of Fetal Pulmonary Sequestrations显示文摘This study aimed to evaluate the prognosis of pulmonary sequestration (PS) by measuring congenital cystic adenomatoid malformation volume ratio (CVR) value in fetal congenital PS.The fetal CVR in 49 cases of fetal PS diagnosed by prenatal ultrasound in Xiangyang No.1 People's Hospital from March 2010 to June 2017 were measured,and the clinical outcomes were observed.According to the prenatal ultrasound CVR value,49 fetuses diagnosed with PS were divided into 2 groups:group 1 with CVR>1.26,and group 2 with CVR<1.26.The incidence rate of fetal edema,respiratory distress symptoms and survival rate were compared between the two groups.The risk factors of the fetal PS were evaluated by single and multiple Logistic regression analysis.The correlation between CVR and fetal prognosis was analyzed.Of the 49 fetuses,there were 34 cases of PS (ILS) type (69.39%,34/49),10 cases of PS (ELS) type I (20.41%,10/49) and 5 cases of PS (ELS) type II (10.20%,5/49).Forty-six cases (93.88%,46/49) were bom alive,there was 1 case (CVR >1.26)(2.04%,1/4刃 of induced abortion,and 2 cases (CVR >1.26)(4.08%,2/49) of stillbirths.In group 1 (w=24),21 cases were bom alive,and the incidence rate of newborn respiratory distress and fetal edema was 100%(21/21) and 79.17%(19/24) respectively.In group 2 (n=25),there were 3 cases (12%,3/25) of newborn respiratory distress,3 cases (12%,3/25) of fetal edema,and the rate of live birth was 100%.There were statistically significant differences between the two groups in the incidence of fetal edema,postpartum respiratory symptoms and survival rate.CVR was a risk factor for PS and was associated with fetal prognosis.CVR in the midtrimester of pregnancy is an effective index to evaluate the prognosis of fetal PS.CVR≥1.26 is associated with an increased risk of fetal edema,infant respiratory distress and intrauterine or postnatal death. | Peng AN Yu WANG Wei FENG Jia-qi ZHANG Yu-xin NING Jia-bao YIN Heng-bo YE Juan SONG Xiao-ni CHEN Jin-zhi XU Qiao-yue HE He ZENG Yang LI Wei YUAN Zi-zhou HE | 2019 | Current Medical Science2019，39，4： | 5 |
| 19 | Comprehensive Analysis of Expressed Sequence Tags from the Pulp of the Red Mutant ‘Cara Cara’ Navel Orange (Citrus sinensis Osbeck)显示文摘Expressed sequence tag(EST) analysis of the pulp of the red-fleshed mutant 'Cara Cara' navel orange provided a starting point for gene discovery and transcriptome survey during citrus fruit maturation.Interpretation of the EST datasets revealed that the mutant pulp transcriptome held a high section of stress responses related genes,such as the type Ⅲ metallothionein-like gene(6.0%),heat shock protein(2.8%),Cu/Zn superoxide dismutase(0.8%),late embryogenesis abundant protein 5(0.8%),etc.133 transcripts were detected to be differentially expressed between the red mutant and its orange-color wild genotype 'Washington' via digital expression analysis.Among them,genes involved in metabolism,defense/stress and signal transduction were statistical overrepresented.Fifteen transcription factors,composed of NAM,ATAF,and CUC transcription factor(NAC);myeloblastosis(MYB);myelocytomatosis(MYC);basic helix-loop-helix(bHLH);basic leucine zipper(bZIP) domain members,were also included.The data reflected the distinct expression profile and the unique regulatory module associated with these two genotypes.Eight differently expressed genes analyzed in digital were validated by quantitative realtime polymerase chain reaction.For structural polymorphism,both simple sequence repeats and single nucleotide polymorphisms(SNP) loci were surveyed;dinucleotide presentation revealed a bias toward AG/GA/TC/CT repeats(52.5%),against GC/CG repeats(0%).SNPs analysis found that transitions(73%) outnumbered transversions(27%).Seventeen potential cultivar-specific and 387 heterozygous SNP loci were detected from 'Cara Cara' and 'Washington' EST pool. | Jun-Li Ye An-Dan Zhu Neng-Guo Tao Qiang Xu Juan Xu Xiu-Xin Deng | 2010 | Journal of Integrative Plant Biology2010，52，10： | 5 |
| 20 | Pterygium epithelium abnormal differentiation related to activation of extracellular signal-regulated kinase signaling pathway in vitro显示文摘AIM: To investigate whether the abnormal differentiation of the pterygium epithelium is related to the extracellular signal-regulated kinase(ERK) signaling pathway in vitro.·METHODS: The expression levels of phosphorylated ERK(P-ERK), keratin family members including K19 and K10 and the ocular master control gene Pax-6 were measured in 16 surgically excised pterygium tissues and 12 eye bank conjunctiva. In colony-forming cell assays,the differences in clone morphology and in K10, K19, PERK and Pax-6 expression between the head and body were investigated. When cocultured with the ERK signaling pathway inhibitor PD98059, the changes in clone morphology, colony-forming efficiency,differentiated marker K10, K19 and Pax-6 expression and P-ERK protein expression level were examined by immunoreactivity and Western blot analysis.· RESULTS: The expression of K19 and Pax-6 decreased in the pterygium, especially in the head. No staining of K10 was found in the normal conjunctiva epithelium, but it was found to be expressed in the superficial cells in the head of the pterygium.Characteristic upregulation of P-ERK was observed by immunohistochemistry. The clone from the head with more differentiated cells in the center expressed more K10, and the clone from the body expressed more K19.The P-ERK protein level increased in the pterygium epithelium compared with conjunctiva and decreased when cocultured with PD98059. The same medium with the ERK inhibitor PD98059 was more effective in promoting clonal growth than conventional medium with3T3 murine feeder layers. It was observed that the epithelium clone co-cultured with the inhibitor had decreased K10 expression and increased K19 and Pax-6 expression.· CONCLUSION: We suggest ERK signaling pathway activation might play a role in the pterygium epithelium abnormal differentiation. | Juan Peng Xiang-Yin Sha Yi Liu Rui-Ming Yang Ye Wen | 2015 | International Journal of Ophthalmology(English edition)2015，8，6： | 5 |
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