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| 1 | Nucleocytoplasmic Trafficking of the Arabidopsis WD40 Repeat Protein XIW1 Regulates ABI5 Stability and Abscisic Acid Responses显示文摘WD40 repeat-containing proteins(WD40 proteins)serve as versatile scaffolds for protein-protein interac-tions,modulating a variety of cellular processes such as plant stress and hormone responses.Here we report the identification of a WD40 protein,XIW1(for XPO1-interacting WD40 protein 1),which positively regulates the abscisic acid(ABA)response in Arabidopsis.XIW1 is located in the cytoplasm and nucleus.We found that it interacts with the nuclear transport receptor XPO1 and is exported by XPO1 from the nucleus.Mutation of XIW1 reduces the induction of ABA-responsive genes and the accumulation of ABA Insensitive 5(ABI5),causing mutant plants with ABA-insensitive phenotypes during seed germination and seedling growth,and decreased drought stress resistance.ABA treatment upregulates the expression of XIW1,and both ABA and abiotic stresses promote XIW1 accumulation in the nucleus,where it interacts with ABI5.Loss of XIW1 function results in rapid proteasomal degradation of ABI5.Taken together,these findings suggest that XIW1 is a nucleocytoplasmic shuttling protein and plays a positive role in ABA responses by interacting with and maintaining the stability of ABI5 in the nucleus. | Xuezhong XU Wang Wan Guobin Jiang Yue Xi Haijian Huang Jiajia Cai Yanan Chang Cheng-Guo Duan Satendra KMangrauthia Xinxiang Peng Jian-Kang Zhu Guohui Zhu | 2019 | Molecular Plant2019，12，12： | 8 |
| 2 | Golgi protein 73, hepatocellular carcinoma and other types of cancers显示文摘Hepatocellular carcinoma(HCC)is one of the most common malignant tumors with a low survival rate.The identification of mechanisms underlying the development of HCC helps uncover cellular and mo-lecular targets for the diagnosis,prevention,and treatment of HCC.Golgi protein 73(GP73)level is up-regulated in HCC patients and potentially can be a therapeutic target.Despite many studies devoted to GP73 as a marker for HCC early diagnosis,there is little discussion about the function of GP73 in HCC tumorigenesis.Given the poor response to currently available HCC therapies,a better understanding of the role of GP73 in HCC may provide a new therapeutic target for HCC.The current paper summarizes the role of GP73 as a diagnostic marker as well as its roles in liver carcinogenesis.Its roles in other types of cancer are also discussed. | Yanan Wang Yu-Jui Yvonne Wan | 2020 | Liver Research2020，4，4： | 5 |
| 3 | Catalyst-Free In Situ Carbon Nanotube Growth in Confined Space via High Temperature Gradient显示文摘Carbonaceous materials,such as graphite,carbon nanotubes(CNTs),and graphene,are in high demand for a broad range of applications,including batteries,capacitors,and composite materials.Studies on the transformation between diferent types of carbon,especially from abundant and low-cost carbon to high-end carbon allotropes,have received surging interest.Here,we report that,without a catalyst or an external carbon source,biomass-derived amorphous carbon and defective reduced graphene oxide(RGO)can be quickly transformed into CNTs in highly confned spaces by high temperature Joule heating.Combined with experimental measurements and molecular dynamics simulations,we propose that Joule heating induces a high local temperature at defect sites due to the corresponding high local resistance.Te resultant temperature gradient in amorphous carbon or RGO drives the migration of carbon atoms and promotes the growth of CNTs without using a catalyst or external carbon source.Our fndings on the growth of CNTs in confned spaces by fast high temperature Joule heating shed light on the controlled transition between diferent carbon allotropes,which can be extended to the growth of other high aspect ratio nanomaterials. | Chaoji Chen Yanan Chen Shuze Zhu Jiaqi Dai Glenn Pastel Yonggang Yao Dapeng Liu Yanbin Wang Jiayu Wan Teng Li Wei Luo Liangbing Hu | 2018 | Research2018，，1： | 2 |
| 4 | Source-specific risk assessment for cadmium in wheat and maize:Towards an enrichment model for China显示文摘Cadmium (Cd) pollution of agricultural soil is of public concern due to its high potential toxicity and mobility.This study aimed to reveal the risk of Cd accumulation in soil and wheat/maize systems,with a specific focus on the source-specific ecological risk,human health risk and Cd enrichment model.For this we investigated more than 6100 paired soil and grain samples with 216 datasets including soil Cd contents,soil pH and grain Cd contents of 85 sites from China.The results showed that mining activities,sewage irrigation,industrial activities and agricultural practices were the critical factors causing Cd accumulation in wheat and maize cultivated sites.Thereinto,mining activities contributed to a higher Cd accumulation risk in the southwest China and Middle Yellow River regions;sewage irrigation influenced the Cd accumulation in the North China Plain.In addition,the investigated sites were classified into different categories by comparing their soil and grain Cd contents with the Chinese soil screening values and food safety values,respectively.Cd enrichment models were developed to predict the Cd levels in wheat and maize grains.The results showed that the models exhibited a good performance for predicting the grain Cd contents among safe and warning sites of wheat (R2=0.61 and 0.72,respectively);while the well-fitted model for maize was prone to the overestimated sites (R2=0.77).This study will provide national viewpoints for the risk assessments and prediction of Cd accumulation in soil and wheat/maize systems. | Zhong Zhuang Qiqi Wang Siyu Huang Andrea Giovanna NinoSavala Yanan Wan Huafen Li Andreas H.Schweiger Andreas Fangmeier Jürgen Franzaring | 2023 | Journal of Environmental Sciences2023，，3： | 1 |
| 5 | Egg-spot matching in common cuckoo parasitism of the oriental reed warbler: effects of host nest availability and egg rejection显示文摘Background: The success of cuckoo parasitism is thought to depend largely on the extent of egg matching between cuckoo and host eggs, since poor-matching cuckoo egg would lead to more frequent egg rejection by the host. In this study, we investigated how egg-spot matching between the Common Cuckoo(Cuculus canorus) and its host, the Oriental Reed Warbler(Acrocephalus orientalis) is affected by the local parasitism rate, nest availability in breeding synchronization and egg rejection.Methods: We used the paired design of parasitized and their nearest non-parasitized nests where breeding occurred simultaneously to compare egg-spot matching. The image analysis was used to compare four eggshell pattern variables, namely spot size, density, coverage on the different areas of egg surface, and the distribution on the whole egg surface. Egg recognition experiments were conducted to test the effect of egg spots on egg rejection by the host.Results: Our results show that much better matching in almost all spot parameters tested on the side of the egg and the spot distribution on the whole egg occurred in parasitized nests than in non-parasitized nests. Matching of spot density between cuckoo and host eggs in parasitized nests increased with the synchronization between temporal availability of nests and the egg-laying period of female cuckoos. Egg recognition experiments in which the warbler eggs were deliberately painted with extra spots led to a significantly higher egg rejection rate(78.3%) than of unpainted eggs.Conclusion: Our data suggest that both the high temporal encounter rate between cuckoo and warbler nests as well as the high egg rejection ability of the host are important factors for egg-spot matching of the cuckoos. | Donglai Li Yanan Ruan Ying Wang Alan K.Chang Dongmei Wan Zhengwang Zhang | 2016 | Chinese Birds2016，，4： | 1 |
| 6 | Study on the Production of Pentachloronitrobenzene Monoclonal Antibody and Its ELISA Kit for Rapid Detection显示文摘[Objectives]This study was conducted to develop an enzyme-linked immunoassay kit that can detect the residual amount of pentachloronitrobenzene in Penaeus vannamei.[Methods]This study was conducted to develop an enzyme-linked immunoassay kit that can detect the residual amount of pentachloronitrobenzene in P.vannamei.[Results]The standard curve range of the kit was 0-8.1μg/L;the detection limit for P.vannamei was 0.912μg/kg;the recovery was 80.6%-103.5%;and the relative standard deviation range within batches was 5.3%-10.1%,and the relative standard deviation range between batches was 6.7%-8.1%.The specificity of the pentachloronitrobenzene monoclonal antibody was relatively good,and the cross-reaction rates with pentachlorophenol,hexachlorobenzene,tetrachlorophthalide,and chlorothalonil were low,all of which did not exceed 30%.The ELISA kit could be stored at 4℃for 12 months,showing good stability.[Conclusions]The detection kit has low cost,short time and small deviation,and is an ideal preliminary screening method. | Yuhua MA Kuo ZHANG Jianxiong ZHANG Fangfang JIA Fangyang HE Yanan CUI Mingyang LI Yuping WAN | 2021 | Agricultural Biotechnology2021，10，5： | 0 |
| 7 | Inhibition of the AKT/mTOR pathway negatively regulates PTEN expression via miRNAs显示文摘PI3K/AKT/mTOR pathway plays important roles in cancer development,and the negative role of PTEN in the PI3K/AKT/mTOR pathway is well known,but whether PTEN can be inversely regulated by PI3K/AKT/mTOR has rarely been reported.Here we aim to investigate the potential regulatory relationship between PTEN and Akt/mTOR inhibition in MEFs.AKT1^(E17K) and TSC2^(–/–)MEFs were treated with the AKT inhibitor MK2206 and the mTOR inhibitors rapamycin and Torin2.Our results reveal that inhibition of AKT or mTOR suppresses PTEN expression in AKT1E17K and TSC2^(–/–)MEFs,but the transcription,subcellular localization,eIF4E-dependent translational initiation or lysosome-and proteasome-mediated degradation of PTEN change little,as shown by the real time PCR,nucleus cytoplasm separation assay and immunofluorescence analysis.Moreover,mTOR suppression leads to augmentation of mouse PTEN-3′UTR-binding miRNAs,including miR-23a-3p,miR-23b-3p,miR-25-3p and miR-26a-5p,as shown by the dual luciferase reporter assay and miRNA array analysis,and miRNA inhibitors collaborately rescue the decline of PTEN level.Collectively,our findings confirm that inhibition of mTOR suppresses PTEN expression by upregulating miRNAs,provide a novel explanation for the limited efficacy of mTOR inhibitors in the treatment of mTOR activation-related tumors,and indicate that dual inhibition of mTOR and miRNA is a promising therapeutic strategy to overcome the resistance of mTOR-related cancer treatment. | Linyan Wan Yanan Wang Jie Li Yani Wang Hongbing Zhang | 2022 | Acta Biochimica et Biophysica Sinica2022，54，11： | 0 |
| 8 | Key considerations on the development of biodegradable biomaterials for clinical translation of medical devices:With cartilage repair products as an example显示文摘With the interdisciplinary convergence of biology,medicine and materials science,both research and clinical translation of biomaterials are progressing at a rapid pace.However,there is still a huge gap between applied basic research on biomaterials and their translational products-medical devices,where two significantly different perspectives and mindsets often work independently and non-synergistically,which in turn significantly increases financial costs and research effort.Although this gap is well-known and often criticized in the biopharmaceutical industry,it is gradually widening.In this article,we critically examine the developmental pipeline of biodegradable biomaterials and biomaterial-based medical device products.Then based on clinical needs,market analysis,and relevant regulations,some ideas are proposed to integrate the two different mindsets to guide applied basic research and translation of biomaterial-based products,from the material and technical perspectives.Cartilage repair substitutes are discussed here as an example.Hopefully,this will lay a strong foundation for biomaterial research and clinical translation,while reducing the amount of extra research effort and funding required due to the dissonance between innovative basic research and commercialization pipeline. | Li Wang Xiaolei Guo Jiaqing Chen Zhen Zhen Bin Cao Wenqian Wan Yuandong Dou Haobo Pan Feng Xu Zepu Zhang Jianmei Wang Daisong Li Quanyi Guo Qing Jiang Yanan Du Jiakuo Yu Boon Chin Heng Qianqian Han Zigang Ge | 2022 | Bioactive Materials2022，7，3： | 0 |
| 9 | 细胞自噬通过清除SQSTM1/p62调控DNA损伤引起的染色质泛素化显示文摘细胞基因组时刻遭受着来自细胞内和细胞外因素的损伤威胁,目前有研究表明细胞自噬的缺失也会导致基因组损伤积累,并进一步导致相关疾病发生。但对于细胞自噬和DNA损伤应答两者之间的关系仍缺乏足够了解。 | Yanan Wang Nan Zhang Luyao Zhang Ran Li Wan Fu Ke Ma Xue Li Lina Wang Jiadong Wang Hongquan Zhang Wei Gu 朱卫国 赵颖 | 2017 | 科学新闻2017，19，4： | 0 |
| 10 | 腐植酸改良剂对水稻幼苗交换性镉及其积累的影响显示文摘食物链中的镉(Cd)对人体有毒,主要是由于植物的积累。通过络合作用形成不溶性复合物来降低生物体内的重金属,是个可行的有机修复方法。先进行42天的土壤培养,随后进行40天的水稻盆栽试验。培养结果显示,在第12天,7种修复材料(腐植酸铵、腐植酸钙、硝基腐植酸钙、腐植酸尿素、腐植酸钾、硝基腐植酸钾和木本泥炭)均显著提高土壤pH值,并使MgCl2中的可交换态Cd含量降低32%以上。 | Yao Yu Sili Yuan Yanan Wan 王振武(译) | 2019 | 腐植酸2019，0，2： | 0 |
| 11 | Interrogating the impact of aggregation-induced emission nanoparticles on in vitro protein stability,ex vivo protein homeostasis,and in vivo biocompatibility显示文摘Aggregation-induced emission(AIE)materials offer promising perspectives in disease diagnosis and therapeutics given their unique optical and photochemical properties.A key step toward translational applications for AIE materials is to systematically and vigorously evaluate their biosafety and biocompatibility.While previous studies focus on cellular viability and toxicity,the impact of AIE materials on detailed stress responses manifesting cellular fitness has been less explored.Herein,this work provides the first piece of evidence to support amphiphilic functionalization of AIE nanoparticles minimizes the deterioration on proteome stability and cellular protein homeostasis(proteostasis).To this end,four scaffolds of AIE molecules were prepared,further functionalized into eight nanoparticles with two amphiphilic shells respectively,and characterized for their physicochemical properties.Thermal shift assay quantitatively demonstrates that AIE materials after amphiphilic functionalization into nanoparticles enhance proteome thermodynamic stability and ameliorate proteome aggregation propensity in cellular lysate,echoed by cell viability and fractionation experiments.Intriguingly,poor polydispersity index(PDI)of functionalized nanoparticles exaggerates their retention and aggregation in the cell.Comparative proteomic analysis uncovers that amphiphilic functionalization of AIE materials can minimize the deterioration of cellular protein homeostasis network.Finally,vigorous interrogation of functionalized AIE nanoparticles in mice model reveals the complexity of factors affecting the biocompatibility profiles in vivo,including materials’size,PDI,and treatment frequencies.Overall,amphiphilic functionalization of AIE materials into nanoparticles is necessary to maintain proteome stability and balance cellular protein homeostasis. | Wang Wan Qun Zhao Biao Jing Congcong Peng Mengdie Wang Yanan Huang Wenhan Jin Bowen Zhong Zhenduo Zhang Xuepeng Dong Zhenming Gao Lihua Zhang Yu Liu | 2022 | Aggregate2022，3，6： | 0 |
| 12 | A Nitride-Reinforced NbMoTaWHfN Refractory High-Entropy Alloy with Potential Ultra-High-Temperature Engineering Applications显示文摘Refractory high-entropy alloys(RHEAs)have promising applications as the new generation of hightemperature alloys in hypersonic vehicles,aero-engines,gas turbines,and nuclear power plants.This study focuses on the microstructures and mechanical properties of the NbMoTaW(HfN)_(x)(x=0,0.3,0.7,and 1.0)RHEAs.The alloys consist of multiple phases of body-centered cubic(BCC),hafnium nitride(HfN),or multicomponent nitride(MN)phases.As the x contents increase,the grain size becomes smaller,and the strength gradually increases.The compressive yield strengths of the NbMoTaWHfN RHEA at ambient temperature,1000,1400,and 1800℃ were found to be 1682,1192,792,and 288 MPa,respectively.The high-temperature strength of this alloy is an inspiring result that exceeds the high temperature and strength of most known alloys,including high-entropy alloys,refractory metals,and superalloys.The HfN phase has a significant effect on strengthening due to its high structural stability and sluggish grain coarsening,even at ultra-high temperatures.Its superior properties endow the NbMoTaWHfN RHEA with potential for a wide range of engineering applications at ultra-high temperatures.This work offers a strategy for the design of high-temperature alloys and proposes an ultra-high-temperature alloy with potential for future engineering applications. | Yixing Wan Yanhai Cheng Yongxiong Chen Zhibin Zhang Yanan Liu Haijun Gong Baolong Shen Xiubing Liang | 2023 | Engineering2023，，11： | 0 |
| 13 | Dual cation doping enabling simultaneously boosted capacity and rate capability of MnO_(2)cathodes for Zn//MnO_(2)batteries显示文摘Aqueous rechargeable Zn//MnO_(2)batteries show promising prospects for grid-scale energy storage due to their intrinsic safety,abundant resource,and potential high performance.Unfortunately,the real capability of these devices is far from satisfactory thanks to the low capacity and sluggish kinetics of the MnO_(2)cathode.Herein,we report a dual cation doping strategy by synthesis of MnO_(2)in the presence of Ti_(3)_(2)X MXenes and Ni^(2+)ions to essentially address these drawbacks.Such a process contributes to a Ti,Ni co-dopedα-MnO_(2)anchored on MXenes.The Ti^(3+)ions incorporated in the framework allow a partial multivalent variation for a large capacity while the Ni^(2+)ions promote the H^(+)transfer within the MnO_(2)matrix via the Grotthuss proton transport manner.As a result,the optimal dual cation doped MnO_(2)exhibits a large reversible capacity of 378 mAh·g-1 at 0.1 C and a high rate capability.Moreover,capacity retention as high as 92%is observed after cycling at 4 C for 1000 times,far superior to many of the previously reported results.This facile strategy demonstrated here may shed new insight into the rational design of electrodes based on high-performance Zn//MnO_(2)batteries. | Chongze Wang Hao Yang Bin Wang Peibin Ding Yi Wan Wenjing Bao Yanan Li Suyan Ma Yang Liu Yukun Lu Han Hu | 2023 | Nano Research2023，16，7： | 0 |
| 14 | Simulation research on collisions between highway corrugated beam guardrails and vehicles based on LS-DYNA显示文摘To explore the safety of highway traffic operations,the vehicle state and guardrail deformation during highway guardrail collisions are simulated and analyzed.The vehicle-guardrail collision is simulated by finite element software such as LS-DYNA and HyperMesh.The vehicle speed settings are 60,80,100 and 120 km/h,and the collision angles are 5°,10°,15°and 20°.The guardrail deformation,vehicle acceleration and energy changes under different collision speeds and angles are studied.The research results show that at the same collision speed,an increase in the collision angle causes more serious damage to the vehicle,a greater transverse displacement of the guardrail,and a greater range of car acceleration fluctuations.When the collision angle is the same,an increase in the collision speed causes greater lateral displacement of the guardrail,a greater vehicle acceleration fluctuation range,and more serious vehicle damage.The results of the study can provide a reference for demonstrating highway guardrail safety. | Yongming He Yanan Wan Kun Wei Jia Feng Cong Quan | 2023 | Digital Transportation and Safety2023，2，1： | 0 |
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