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| 1 | AXL is a candidate receptor for SARS-CoV-2 that promotes infection of pulmonary and bronchial epithelial cells显示文摘The current coronavirus disease 2019(COVID-19)pandemic presents a global public health challenge.The viral pathogen responsible,severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),binds to the host receptor ACE2 through its spike(S)glycoprotein,which mediates membrane fusion and viral entry.Although the role of ACE2 as a receptor for SARS-CoV-2 is clear,studies have shown that ACE2 expression is extremely low in various human tissues,especially in the respiratory tract.Thus,other host receptors and/or co-receptors that promote the entry of SARS-CoV-2 into cells of the respiratory system may exist.In this study,we found that the tyrosine-protein kinase receptor UFO(AXL)specifically interacts with the N-terminal domain of SARS-CoV-2 S.Using both a SARS-CoV-2 virus pseudotype and authentic SARS-CoV-2,we found that overexpression of AXL in HEK293T cells promotes SARS-CoV-2 entry as efficiently as overexpression of ACE2,while knocking out AXL significantly reduces SARS-CoV-2 infection in HI 299 pulmonary cells and in human primary lung epithelial cells.Soluble human recombinant AXL blocks SARS-CoV-2 infection in cells expressing high levels of AXL.The AXL expression level is well correlated with SARS-CoV-2 S level in bronchoalveolar lavage fluid cells from COVID-19 patients.Taken together,our findings suggest that AXL is a novel candidate receptor for SARS-CoV-2 which may play an important role in promoting viral infection of the human respiratory system and indicate that it is a potential target for future clinical intervention strategies. | Shuai Wang Zongyang Qiu Yingnan Hou Xiya Deng Wei Xu Tingting Zheng Peihan Wu Shaofang Xie Weixiang Bian Chong Zhang Zewei Sun Kunpeng Liu Chao Shan Aifu Lin Shibo Jiang Youhua Xie Qiang Zhou Lu Lu Jing Huang Xu Li | 2021 | Cell Research2021，31，2： | 39 |
| 2 | Probing the fractional topological charge of a vortex light beam by using dynamic angular double slits显示文摘Vortex beams with fractional topological charge(FTC) have many special characteristics and novel applications.However, one of the obstacles for their application is the difficulty of precisely determining the FTC of fractional vortex beams. We find that when a vortex beam with an FTC illuminates a dynamic angular double slit(ADS), the far-field interference patterns that include the information of the FTC of the beam at the angular bisector direction of the ADS vary periodically. Based on this property, a simple dynamic ADS device and data fitting method can be used to precisely measure the FTC of a vortex light beam with an error of less than 5%. | Jing Zhu Pei Zhang Dongzhi Fu Dongxu Chen Ruifeng Liu Yingnan Zhou Hong Gao Fuli Li | 2016 | Photonics Research2016，4，5： | 4 |
| 3 | Analysis of Expression Profiles of Long Noncoding RNAs and mRNAs in A549 Cells Infected with H3N2 Swine Influenza Virus by RNA Sequencing显示文摘Long noncoding RNAs(lncRNAs)participate in regulating many biological processes.However,their roles in influenza A virus(IAV)pathogenicity are largely unknown.Here,we analyzed the expression profiles of lncRNAs and mRNAs in H3N2-infected cells and mock-infected cells by high-throughput sequencing.The results showed that 6129 lncRNAs and 50,031 mRNA transcripts in A549 cells displayed differential expression after H3N2 infection compared with mock infection.Among the differentially expressed lncRNAs,4963 were upregulated,and 1166 were downregulated.Functional annotation and enrichment analysis using gene ontology and Kyoto Encyclopedia of Genes and Genomes databases(KEGG)suggested that target genes of the differentially expressed lncRNAs were enriched in some biological processes,such as cellular metabolism and autophagy.The up-or downregulated lncRNAs were selected and further verified by quantitative real-time polymerase chain reaction(RT-qPCR)and reverse transcription PCR(RT-PCR).To the best of our knowledge,this is the first report of a comparative expression analysis of lncRNAs in A549 cells infected with H3N2.Our results support the need for further analyses of the functions of differentially expressed lncRNAs during H3N2 infection. | Yina Zhang Tianqi Yu Yingnan Ding Yahui Li Jing Lei Boli Hu Jiyong Zhou | 2020 | Virologica Sinica2020，35，2： | 2 |
| 4 | GACNet: A Generative Adversarial Capsule Network for Regional Epitaxial Traffic Flow Prediction显示文摘With continuous urbanization,cities are undergoing a sharp expansion within the regional space.Due to the high cost,the prediction of regional traffic flow is more difficult to extend to entire urban areas.To address this challenging problem,we present a new deep learning architecture for regional epitaxial traffic flow prediction called GACNet,which predicts traffic flow of surrounding areas based on inflow and outflow information in central area.The method is data-driven,and the spatial relationship of traffic flow is characterized by dynamically transforming traffic information into images through a two-dimensional matrix.We introduce adversarial training to improve performance of prediction and enhance the robustness.The generator mainly consists of two parts:abstract traffic feature extraction in the central region and traffic prediction in the extended region.In particular,the feature extraction part captures nonlinear spatial dependence using gated convolution,and replaces the maximum pooling operation with dynamic routing,finally aggregates multidimensional information in capsule form.The effectiveness of the method is evaluated using traffic flow datasets for two real traffic networks:Beijing and New York.Experiments on highly challenging datasets show that our method performs well for this task. | Jinyuan Li Hao Li Guorong Cui Yan Kang Yang Hu Yingnan Zhou | 2020 | Computers, Materials & Continua2020，，8： | 1 |
| 5 | Alignment measurement of two-dimensional zero-reference marks显示文摘 | Wang Yingnan Chen Yuhang | 2006 | Precision Engineering2006，30，： | 1 |
| 6 | GaN-based ultraviolet microdisk laser diode grown on Si显示文摘This work reports a demonstration of electrically injected GaN-based near-ultraviolet microdisk laser diodes with a lasing wavelength of 386.3 nm at room temperature. The crack-free laser structure was epitaxially grown on Si substrates using an Al-composed down-graded Al N/AlGaN multilayer buffer to mitigate the mismatches in the lattice constant and coefficient of thermal expansion, and processed into “sandwich-like” microdisk structures with a radius of 12 μm. Air-bridge electrodes were successfully fabricated to enable the device electrical characterization. The electrically pumped lasing of the as-fabricated microdisk laser diodes was evidenced by the rapid narrowing down of electroluminescence spectra and dramatic increase in the light output power, as the current exceeded the threshold of 248 mA. | JIN WANG MEIXIN FENG RUI ZHOU QIAN SUN JIANXUN LIU YINGNAN HUANG YU ZHOU HONGWEI GAO XINHE ZHENG MASAO IKEDA HUI YANG | 2019 | Photonics Research2019，7，6： | 0 |
| 7 | Surveillance system-based physician reporting of pneumonia of unknown etiology in China:A cross-sectional study显示文摘Objective:To describe the current reporting of pneumonia of unknown etiology(PUE)and factors that affect reporting by clinicians in China using the PUE surveillance system in order to provide a reference for improving PUE reporting rates in the future.Methods:Clinicians were recruited via the Sojump platform and requested to complete an anonymous self-administered questionnaire.Multivariate logistic regression analysis was used to assess factors influencing clinicians’reporting activities.Results:This study showed a low PUE case reporting rate and a poor understanding of PUE reporting among the investigated clinicians.Of the 136 clinicians who had diagnosed PUE cases,multivariate logistic regression analysis results showed that clinicians who had attended in-hospital training were more likely to report PUE than those who had not(OR 4.48,95%CI 1.49-13.46).Clinicians with an expert panel on PUE in their hospital were more likely to report PUE cases than those without(OR 5.46,95%CI 1.85-16.11).Conclusions:There is a need to promote and reinforce PUE surveillance system training among medical staff.In addition,PUE testing technologies in hospital laboratories should be upgraded,especially in primary and unclassified hospitals,to increase surveillance efficiency and improve PUE reporting rates. | Hongchun Du Yajuan Zhu Yan Chen Shidan Zhou Jiani Tong Yingnan Deng Dingmei Zhang | 2022 | Asian Pacific Journal of Tropical Medicine2022，15，4： | 0 |
| 8 | The Cost Analysis on the Law Regulation for Recycling Economy显示文摘The paper analyzes the external economy of pollution. Two different cost models of enterprises are established. From the point of enterprises and the government, the measures of eliminating the externality are discussed, and the limits to the measures are analyzed. | Zhou Yingnan Yang Lin Wang Xuedong | 2005 | Ecological Economy2005，1，4： | 0 |
| 9 | Yiqi Qingre Xiaozheng formula protects against diabetic nephropathy by restoring autophagy in mice显示文摘Objective:To evaluate the protective effect of Yiqi Qingre Xiaozheng formula(YQQRXZF)via the regulation of autophagy in diabetic nephropathy(DN)mice induced by injection of streptozotocin(STZ)after high fat diet(HFD).Methods:The composition of YQQRXZF was analyzed by high performance liquid chromatographyelectrospray ionization/mass spectrometry(HPLC-ESI/MSn).The DN model was induced by intraperitoneally injection of 50 mg/kg STZ within 5 days,followed by HFD feeding for 8 weeks in C57BL/6J mice.Mice were randomly separated into DN group,YQQRXZF group,irbesartan group,and control group.Blood glucose was calculated,and body weight was measured every 2 weeks.An enzyme-linked immunosorbent assay was used to measure the urinary albumin-to-creatinine ratio(uACR)before and after treatment,and the serum concentrations of total cholesterol(TC),low-density lipoprotein(LDL),triglycerides(TG),high-density lipoprotein(HDL),blood urea nitrogen(BUN),and serum creatinine(Scr)were measured.In addition,hematoxylin-eosin(H&E)staining,periodic acid-Schiff(PAS)staining,Masson's trichrome staining,and transmission electron microscopy(TEM)were used to observe pathological changes in renal tissue.Autophagy levels were determined by immunofluorescence staining.Results:In this study,21 dominant chemical constituents were identified in YQQRXZF.The treatment group reduced u ACR in a more significant way than the DN group(P=.018).The treatment group reduced TC,TG,and LDL concentrations after YQQRXFF treatment(P=.021,P=.014 and P=.026,respectively).H&E,PAS,and Masson staining showed that pathological damage to mice kidneys improved,the volume of renal glomeruli was reduced,and glomerular sclerosis and fibrosis were reduced.Immunofluorescence analysis revealed that expressions of LC3-Ⅱprotein increased in the treatment group(P=.013).In contrast,the protein expressions of P62 were reduced after treatment(P=.025). | Na Sun Nan Yang Jingwei Zhou Ying He Jian Wang Yingnan Liang Run Dai Jiajia Bai Zhenjie Chen | 2023 | Journal of Traditional Chinese Medical Sciences2023，10，3： | 0 |
| 10 | Validation studies of the Para DNA^(■) Intelligence System with artificial evidence items显示文摘Short tandem repeat(STR)profiling is one of the mostly used systems for forensic applications.In certain circumstances,STR profiling is time-consuming and costly,which potentially leads to delays in criminal investigations.LGC(Laboratory of the Government Chemist,UK)Forensics has developed a robust STR profiling platform called the ParaDNAVR Intelligence Test System which can provide early tactical intelligence and aid investigators in making informed decisions on sample prioritization for detection.Here,we validated the ParaDNA^(■) intelligence test for its application in forensic cases using a range of mock evidence items following guidelines set by the Scientific Working Group on DNA Analysis Methods(SWGDAM).Specifically,we tested the sensitivity and accuracy of the ParaDNA intelligence test,as well as the success rates for detecting mock samples and for use in case scenarios.Our findings demonstrate that the ParaDNA intelligence test generates useful DNA profiles,especially for samples such as blood,saliva,and semen that contain ample DNA,indicating the benefits of including ParaDNA as a prior step in forensic STR profiling pipelines. | Min Li Ruiyang Tao Wei Zhou Yanan Li Meng Meng Yilun Zhang Linsheng Yu Liqin Chen Yingnan Bian Chengtao Li | 2021 | Forensic Sciences Research2021，6，1： | 0 |
| 11 | Thanatomicrobiome composition profiling as a tool for forensic investigation显示文摘Thanatomicrobiome,or the postmortem microbiome,has been recognized as a useful microbial marker of the time and location of host death.In this mini-review,we compare the experimental methods commonly applied to thanatomicrobiome studies to the state-of-the-art methodologies in the microbiome field.Then,we review present findings in thanatomicrobiome studies,focusing on the diversity of the thanatomicrobiome composition and prediction models that have been proposed.Finally,we discuss potential improvements and future directions of the field. | Wei Zhou Yingnan Bian | 2018 | Forensic Sciences Research2018，3，2： | 0 |
| 12 | Light output improvement of GaN-based light-emitting diodes grown on Si (111) by a via-thin-film structure显示文摘This work reports the fabrication of via-thin-film light-emitting diode(via-TF-LED)to improve the light output power(LOP)of blue/white Ga N-based LEDs grown on Si(111)substrates.The as-fabricated via-TF-LEDs were featured with a roughened n-GaN surface and the p-GaN surface bonded to a wafer carrier with a silver-based reflective electrode,together with an array of embedded n-type via pillar metal contact from the p-Ga N surface etched through the multiple-quantum-wells(MQWs)into the n-Ga N layer.When operated at 350 m A,the via-TF-LED gave an enhanced blue LOP by 7.8%and over 3.5 times as compared to the vertical thin-film LED(TF-LED)and the conventional lateral structure LED(LS-LED).After covering with yellow phosphor that converts some blue photons into yellow light,the via-TF-LED emitted an enhanced white luminous flux by 13.5%and over 5times,as compared with the white TF-LED and the white LS-LED,respectively.The significant LOP improvement of the via-TF-LED was attributed to the elimination of light absorption by the Si(111)epitaxial substrate and the finger-like n-electrodes on the roughened emitting surface. | Zengcheng LI Bo Feng Biao Deng Legong Liu Yingnan Huang Meixin Feng Yu Zhou Hanmin Zhao Qian Sun Huaibing Wang Xiaoli Yang Hui Yang | 2018 | Journal of Semiconductors2018，39，4： | 0 |
| 13 | Se-sensitized NIR hot band absorption photosensitizer for anti-Stokes excitation deep photodynamic therapy显示文摘Conventional anti-Stokes materials-involved deep photodynamic therapy(dPDT)requires much high-intensity irradiance due to low photosensitization efficiency.Herein,we proposed a'booster effector'approach to construct highly efficient hot band absorption phototherapeutics for low/biosafety power anti-Stokes light-triggered d PDT.Se,as'booster effector',was introduced into hot band absorption luminophores(HBAs),which not only significantly facilitated intersystem crossing,but also simultaneously enhanced hot band excitation efficiency atν808,as a result successfully enabling excellent photogenerated singlet oxygen capability of HBAs under ultra-low power anti-Stokes excitation(10 mW cm^(-2)in vitro).As far as we know,such low laser power-initiated photosensitization activity has never been reported in the existing anti-Stokes material systems.Importantly,FUC-Se ME can self-assemble into uniform nanospheres in water,greatly boosting cellular uptake(>25-fold larger than FUC-Se),and achieve superior cancer-killing effect(808 nm,10 mW cm^(-2),5 min,the half-maximal inhibitory concentration IC50=1.36μM).After further PEGylation with folate-attached polymer,the resultant FUC-Se ME@FA can effectively enrich at the tumor(signal-to-background ratio,10).Under safety irradiation(330 mW cm^(-2)),FUC-Se ME@FA effectively inhibits deep-seated tumor progression(the tumor growth inhibition rate,84%).This work provides a successful paradigm,possibly being more clinically beneficial than conventional anti-Stokes materials. | Dandan Ma Hui Bian Saran Long Panwang Zhou Ruisong Tian Yingnan Wu Haoying Ge Mingle Li Jianjun Du Jiangli Fan Yukui Zhang Xiaojun Peng | 2022 | Science China Chemistry2022，65，3： | 0 |
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