|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Expression, purification, and functional characterization of recombinant PTD-SARA显示文摘为蛋白质是的受体激活(SARA ) 的 Smad 锚为在支持发信号的小径的 fibrotic 起一个重要作用的 Smad2/3 的一个有约束力的搭挡由转变生长 factor-1 (TGF-1 ) 开始了。C 终端 SARA 的 665750 aa 包括 Smad 有约束力的领域(SBD ) 。由通过 SBD 的直接相互作用, SARA 禁止 Smad2/3 phosphorylation 并且堵住在 Smad2/3 和 Smad4 之间的相互作用,从而制止纤维变性的过程。在这研究,我们基于 SBD 顺序构造了 SARA 肽 aptamer。recombinant SARA aptamer,与一个蛋白质 transduction 领域(PTD-SARA ) 熔化了,被克隆,从 E 净化了。coli,并且第一次描绘了。编码顺序的全身的 PTD-SARA,与 E 创造了。coli 赞成了 codons,被克隆进 pQE-30 向量,并且 recombinant plasmid 被转变成 M15 紧张。在 Isopropyl -D-1-Thiogalactopyranoside (IPTG ) 以后正式就职和 Ni2+ 亲密关系纯化, recombinant PTD-SARA 被定序的 immunoblotting 和蛋白质 N 终端进一步识别。Epifluorescence 显微镜学表明 recombinant PTD-SARA 比 SARA 更高效地被变成细胞质和原子核。而且, recombinant PTD-SARA 被发现 E-cadherin 和下面调整的水平到起来调整 -SMA 和 phospho-Smad3 的层次更高效地比 SARA (P < 0.05 ) 。我们的工作探索了一个方法获得 recombinant PTD-SARA 蛋白质。recombinant PTD-SARA 熔化蛋白质能进入 HK2 房间(使不朽的近似小管上皮的房间线) 更高效地比 SARA 蛋白质和颠倒被 TGF-1 比 SARA 蛋白质更有效地导致的肾的 epithelial-to-mesenchymal transdifferentiation 过程。Recombinant PDT-SARA 是可能的是肾的纤维变性的临床的预防和处理的一个潜在的候选人。 | Chen Huang Rui Du Peng Zhang Hua Meng Huiwei Jia Yang Song Man Li Yingqi Zhang Shiren Sun | 2011 | Acta Biochimica et Biophysica Sinica2011，43，2： | 4 |
| 2 | Monascus vinegar alleviates high-fat-diet-induced inflammation in rats by regulating the NF-κB and PI3K/AKT/mTOR pathways显示文摘Monascus vinegar(MV) is a typical fermented food with various health-promoting effects. This study aimed to evaluate the role of MV in alleviating high-fat-diet-induced inflammation in rats with hyperlipidemia and elucidate the possible regulatory mechanisms. In the study, serum lipid profiles, liver pathology and liver inflammatory cytokines were analyzed in hyperlipidemia rats with MV(0.5 mL/kg mb, 2 mL/kg mb). Results showed that the administration of MV alleviated dyslipidemia by decreasing the serum and liver levels of triglyceride and total cholesterol. Increase in hepatic lipase and carnitine palmitoyl transferase 1(CPT-1)levels and decrease in hepatocyte steatosis, nephritis, and intestinal tissue injury in the HD group showed that high-dose MV can significantly suppress hepatic lipid accumulation and steatosis. In addition, compared with the model(MOD) group, the HD group showed significantly down-regulated the level of serum or hepatic alanine aminotransferase(ALT), aspartate aminotransferase(AST), CPT-1, interleukin(IL)-2, IL-6, IL-12,and tumor necrosis factor α(TNF-α). Moreover, the HD group showed repressed hepatic nuclear factor κB(NF-κB) pathway and inactivated phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)/mammalian target of rapamycin(mTOR) pathway mitigated liver inflammation. Similar results were obtained from cell experiments. Collectively, these findings revealed that MV might attenuate high-fat-diet-induced inflammation by inhibiting the NF-κB and PI3K/Akt/mTOR pathways. | Huanmei Meng Jia Song Bingqian Fan Yingqi Li Jiaojiao Zhang Jinping Yu Yu Zheng Min Wang | 2022 | Food Science and Human Wellness2022，11，4： | 2 |
| 3 | Efficient and high-fidelity base editor with expanded PAM compatibility for cytidine dinucleotide显示文摘Cytidine base editor(CBE),which is composed of a cytidine deaminase fused to Cas9 nickase,has been widely used to induce C-to-T conversions in a wide range of organisms.However,the targeting scope of current CBEs is largely restricted to protospacer adjacent motif(PAM)sequences containing G,T,or A bases.In this study,we developed a new base editor termed“nNme2-CBE”with excellent PAM compatibility for cytidine dinucleotide,significantly expanding the genome-targeting scope of CBEs.Using nNme2-CBE,targeted editing efficiencies of 29.0%-55.0%and 17.3%—52.5%were generated in human cells and rabbit embryos,respectively.In contrast to conventional nSp-CBE,the nNme2-CBE is a natural high-fidelity base editing platform with minimal DNA off-targeting detected in vivo.Significantly increased efficiency in GC context and precision were determined by combining nNme2Cas9 with rationally engineered cytidine deaminases.In addition,the Founder rabbits with accurate single-base substitutions at Fgf5 gene loci were successfully generated by using the nNme2-CBE system.These novel nNme2-CBEs with expanded PAM compatibility and high fidelity will expand the base editing toolset for efficient gene modification and therapeutic applications. | Zhiquan Liu Siyu Chen Yingqi Jia Huanhuan Shan Mao Chen Yuning Song Liangxue Lai Zhanjun Li | 2021 | Science China(Life Sciences)2021，64，8： | 2 |
| 4 | Regulating off-centering distortion maximizes photoluminescence in halide perovskites显示文摘Metal halide perovskites possess unique atomic and electronic configurations that endow them with high defect tolerance and enable high-performance photovoltaics and optoelectronics.Perovskite light-emitting diodes have achieved an external quantum efficiency of over 20%.Despite tremendous progress,fundamental questions remain,such as how structural distortion affects the optical properties.Addressing their relationships is considerably challenging due to the scarcity of effective diagnostic tools during structural and property tuning as well as the limited tunability achievable by conventional methods.Here,using pressure and chemical methods to regulate the metal off-centering distortion,we demonstrate the giant tunability of photoluminescence(PL)in both the intensity(>20 times)and wavelength(>180 nm/GPa)in the highly distorted halide perovskites[CH_(3)NH_(3)GeI_(3),HC(NH_(2))2GeI_(3),and Cs Ge I_(3)].Using advanced in situ high-pressure probes and first-principles calculations,we quantitatively reveal a universal relationship whereby regulating the level of off-centering distortion towards 0.2 leads to the best PL performance in the halide perovskites.By applying this principle,intense PL can still be induced by substituting CH_(3)NH_(3)^(+)with Cs^(+)to control the distortion in(CH_(3)NH_(3))_(1-x)Cs_(x)GeI_(3),where the chemical substitution plays a similar role as external pressure.The compression of a fully substituted sample of CsGe I_(3)further tunes the distortion to the optimal value at 0.7 GPa,which maximizes the emission with a10-fold enhancement.This work not only demonstrates a quantitative relationship between structural distortion and PL property of the halide perovskites but also illustrates the use of knowledge gained from high-pressure research to achieve the desired properties by ambient methods. | Xujie Lv Constantinos Stoumpos Qingyang Hu Xuedan Ma Dongzhou Zhang Songhao Guo Justin Hoffman Kejun Bu Xiaofeng Guo Yingqi Wang Cheng Ji Haijie Chen Hongwu Xu Quanxi Jia Wenge Yang Mercouri G.Kanatzidis Ho-Kwang Mao | 2021 | National Science Review2021，8，9： | 1 |
| 5 | Mettl13 protects against cardiac contractile dysfunction by negatively regulating C-Cbl-mediated ubiquitination of SERCA2a in ischemic heart failure显示文摘Ischemic heart failure(HF)remains a leading cause of morbidity and mortality.Maintaining homeostasis of cardiac function and preventing cardiac remodeling deterioration are critical to halting HF progression.Methyltransferase-like protein 13(Mettl13)has been shown to regulate protein translation efficiency by acting as a protein lysine methyltransferase,but its role in cardiac pathology remains unexplored.This study aims to characterize the roles and mechanisms of Mettl13 in cardiac contractile function and HF.We found that Mettl13 was downregulated in the failing hearts of mice post-myocardial infarction(MI)and in a cellular model of oxidative stress.Cardiomyocyte-specific overexpression of Mettl13 mediated by AAV9-Mettl13 attenuated cardiac contractile dysfunction and fibrosis in response to MI,while silencing of Mettl13 impaired cardiac function in normal mice.Moreover,Mettl13 overexpression abrogated the reduction in cell shortening,Ca^(2+)transient amplitude and SERCA2a protein levels in the cardiomyocytes of adult mice with MI.Conversely,knockdown of Mettl13 impaired the contractility of cardiomyocytes,and decreased Ca^(2+)transient amplitude and SERCA2a protein expression in vivo and in vitro.Mechanistically,Mettl13 impaired the stability of c-Cbl by inducing lysine methylation of c-Cbl,which in turn inhibited ubiquitination-dependent degradation of SERCA2a.Furthermore,the inhibitory effects of knocking down Mettl13 on SERCA2a protein expression and Ca^(2+)transients were partially rescued by silencing c-Cbl in H_(2)O_(2)-treated cardiomyocytes.In conclusion,our study uncovers a novel mechanism that involves the Mettl13/c-Cbl/SERCA2a axis in regulating cardiac contractile function and remodeling,and identifies Mettl13 as a novel therapeutic target for ischemic HF. | Shuting Yu ZhiYong Sun Xiuzhu Wang Tiantian Ju Changhao Wang Yingqi Liu Zhezhe Qu KuiWu Liu Zhongting Mei Na Li Meixi Lu Fan Wu Min Huang Xiaochen Pang Yingqiong Jia Ying Li Yaozhi Zhang Shunkang Dou Jianhao Jiang Xin Li Baofeng Yang Weijie Du | 2023 | Science China(Life Sciences)2023，66，12： | 0 |
| 6 | Dynamic Mechanical Characteristics and Damage Evolution Model of Granite显示文摘By using the technique of the split Hopkinson pressure bar( SHPB),impact tests at different stress wavelengths( 0. 8-2. 0 m) and strain rates( 20-120 s^(-1)) were conducted to study the dynamic mechanical properties and damage accumulation evolution lawof granite. Test results showthat the dynamic compressive strength and strain rate of granite have a significantly exponential correlation;the relationship between peak strain and strain rate is approximately linear,and the increase of wavelengths generally makes the level of peak strain uplift. The multiple-impacts test at a lowstrain rate indicates that at the same wavelength,the cumulative damage of granite shows an exponential increasing form with the increase of strain rate; when keeping the increase of strain rate constant and increasing the stress wavelength,the damage accumulation effect of granite is intensified and still shows an exponential increasing form; under the effect of multiple impacts,the damage development trend of granite is similar overall,but the increase rate is accelerating. Therefore the damage evolution model was established on the basis of the exponential function while the physical meaning of parameters in the model was determined. The model can reflect the effect of the wave parameters and multiple impacts. The validity of the model and the physical meaning of the parameters were verified by the test,which further offer a reference for correlational research and engineering application for the granite. | Shuaifeng Wu Yingqi Wei Hong Cai Bei Jia Dianshu Liu | 2018 | Journal of Beijing Institute of Technology2018，27，2： | 0 |
| 7 | Targeted mutagenesis in rabbit using an engineered BhCas12b variant显示文摘Dear Editor,The clustered regularly interspaced short palindromic repeat(CRISPR)and CRISPR-associated protein(CRISPR–Cas)system has exhibited powerful abilities to manipulate genomes of animals and plants(Knott and Doudna,2018).Up to now,numerous Cas nucleases have been harnessed for genome editing in human cells,such as Cas9,Cas12a(also known as Cpf1),and Cas12b(also termed C2c1).Cas12b,a Class 2 type V-B CRISPR system,generates staggered double-strand breaks(DSBs)in the target DNA(Stella et al.,2017)and recognizes a distal 5′-T-rich protospacer adjacent motif(PAM)sequence(Shmakov et al.,2015),making it a complement to Cas9(recognizing 5′-NGG-3′PAM)in genome editing.Three Cas12b nucleases have been engineered for targeted genome editing in mammals or plants:BhCas12b v4(Strecker et al.,2019),BvCas12b(Strecker et al.,2019),and AaCas12b(Teng et al.,2018).However,they have not been compared parallelly with each other.In this study,we compared the three Cas12b proteins for genome editing in mammalian cells. | Yingqi Jia Tian Wang Ding Zhao Zhiquan Liu Tingting Sui Siyu Chen Jinze Li Liangxue Lai Zhanjun Li | 2022 | Journal of Molecular Cell Biology2022，14，12： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员