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| 1 | Identification and Characterization of microRNA during Bemisia tabaci Infestations in Solanum lycopersicum and Solanum habrochaites显示文摘MicroRNAs(miRNAs) are a class of small non-coding RNAs that can regulate target gene expression during many plant growth and development processes. In recent years, several studies identified the miRNAs involved in fruit development, leaf development, and abiotic responses in tomato. However, little is known about the miRNAs that respond to insect attack. Here, miRNAs were identified by high-throughput sequencing at different stages after infections by the whitefly Bemisia tabaci in Solanum lycopersicum and Solanum habrochaites, which are susceptible and resistant to whitefly, respectively. A total of 44 known miRNA families were identified, and 33 were shared between the two species. Among these, 13 miRNA families were newly reported in tomato. After strict filtering, some novel miRNAs were also discovered. The global expression patterns of the miRNAs exhibited were different between the two species, reflecting their different responses and resistance levels to whitefly attack. Some of the predicted target genes of differentially expressed miRNAs may be involved in responding to, and defending against, diseases and insects. Thus, plant miRNAs are important in the responses to, and resistance against, insects and provide a useful resource for further investigations into the mechanism of miRNA-mediated plant–insect interactions. | WANG Ketao SU Xiaomei CUI Xia DU Yongchen ZHANG Shuaibin GAO Jianchang | 2018 | Horticultural Plant Journal2018，4，2： | 6 |
| 2 | Identification of Regulatory DNA Elements Using Genome-wide Mapping of DNase I Hypersensitive Sites during Tomato Fruit Development显示文摘开发并且西红柿水果成熟被 transcriptional 规定精确控制,它取决于规章的蛋白质的安排可接近性到倡导者和另外的 cis 规章的 DNA 元素。基因表示上的这可接近性和它的效果在定义发展进程起一个主要作用。为了理解规章的机制和词法、解剖的功能的元素 modulating,在水果开发期间变化,我们产生了 DNase 的染色体宽的高分辨率的地图我从西红柿栽培变种 “Moneymaker” 的水果纸巾的过分敏感的地点(DHS )在 20 点,天张贴开花期以及裂缝舞台。由越过水果发展阶段探索 DHS 的变化,我们指出了与发展特定的基因有关的最可能的过分敏感的地点。由在维生素酸 biosynthetic 小径在这些发展特定的基因或基因的 DHS 上检测有约束力的主题,我们揭示了贡献协调植物成熟的基因抄写的普通规章的元素并且专业化新陈代谢的小径。我们的结果贡献一更好涉及西红柿水果开发的基因的规章的动力学理解并且成熟。 | Zhengkun Qiu Ren Li Shuaibin Zhang Ketao Wang Meng Xu Jiayang Li Yongchen Du Hong YU Xia Cui | 2016 | Molecular Plant2016，9，8： | 6 |
| 3 | A putative bHLH transcription factor is a candidate gene for male sterile 32,a locus affecting pollen and tapetum development in tomato显示文摘The tomato(Solanum lycopersicum)male sterile 32(ms32)mutant has been used in hybrid seed breeding programs largely because it produces no pollen and has exserted stigmas.In this study,histological examination of anthers revealed dysfunctional pollen and tapetum development in the ms32 mutant.The ms32 locus was fine mapped to a 28.5 kb interval that encoded four putative genes.Solyc01g081100,a homolog of Arabidopsis bHLH10/89/90 and rice EAT1,was proposed to be the candidate gene of MS32 because it contained a single nucleotide polymorphism(SNP)that led to the formation of a premature stop codon.A codominant derived cleaved amplified polymorphic sequence(dCAPS)marker,MS32D,was developed based on the SNP.Real-time quantitative reverse-transcription PCR showed that most of the genes,which were proposed to be involved in pollen and tapetum development in tomato,were downregulated in the ms32 mutant.These findings may aid in marker-assisted selection of ms32 in hybrid breeding programs and facilitate studies on the regulatory mechanisms of pollen and tapetum development in tomato. | Xiaoyan Liu Mengxia Yang Xiaolin Liu Kai Wei Xue Cao Xiaotian Wang Xiaoxuan Wang Yanmei Guo Yongchen Du Junming Li Lei Liu Jinshuai Shu Yong Qin Zejun Huang | 2019 | Horticulture Research2019，6，1： | 3 |
| 4 | Age-related Resistance and the Defense Signaling Pathway of Ph-3 Gene Against Phytophthora infestans in Tomatoes显示文摘Resistance(R) genes against plant pathogens often have age-related resistance(ARR) effects. However, the mechanism involved in this phenomenon remains unknown. In this paper, Solanum lycopersicum ‘CLN2037B' and S. pimpinellifolium ‘L3708' harboring the Ph-3 gene, as well as S. habrochaites ‘LA2099', ‘LA1777' and ‘LA1033' harboring quantitative trait loci(QTLs), were tested to investigate age-related resistance against late blight(LB; caused by Phytophthora infestans) in the three-leaf stage of the plants. The results demonstrated that the QTL-related LB resistance showed the same age-related resistance as the Ph-3-mediated resistance at the six- and nine-leaf stages compared with the three-leaf stage. This indicated that there is a common defense mechanism in tomatoes against P. infestans via ARR. In addition, we combined ethylene(ET), salicylic acid(SA) and jasmonic acid(JA) mutants with virus-induced gene silencing(VIGS) to study the Ph-3-dependent resistance signaling pathway. The results showed that ethylene and salicylic acid, but not jasmonic acid, are involved in the LB resistance mediated by the Ph-3 gene. | Sayed Rashad Ali Shah LI Tao CHI Haijuan LIU Lei ZHENG Zheng LI Junming DU Yongchen | 2015 | Horticultural Plant Journal2015，1，2： | 1 |
| 5 | OBV(obscure vein),a C_(2)H_(2)zinc finger transcription factor,positively regulates chloroplast development and bundle sheath extension formation in tomato(Solanum lycopersicum)leaf veins显示文摘Leaf veins play an important role in plant growth and development,and the bundle sheath(BS)is believed to greatly improve the photosynthetic efficiency of C_(4) plants.The OBV mutation in tomato(Solanum lycopersicum)results in dark veins and has been used widely in processing tomato varieties.However,physiological performance has difficulty explaining fitness in production.In this study,we confirmed that this mutation was caused by both the increased chlorophyll content and the absence of bundle sheath extension(BSE)in the veins.Using genome-wide association analysis and map-based cloning,we revealed that OBV encoded a C_(2)H_(2) L domain class transcription factor.It was localized in the nucleus and presented cell type-specific gene expression in the leaf veins.Furthermore,we verified the gene function by generating CRISPR/Cas9 knockout and overexpression mutants of the tomato gene.RNA sequencing analysis revealed that OBV was involved in regulating chloroplast development and photosynthesis,which greatly supported the change in chlorophyll content by mutation.Taken together,these findings demonstrated that OBV affected the growth and development of tomato by regulating chloroplast development in leaf veins.This study also provides a solid foundation to further decipher the mechanism of BSEs and to understand the evolution of photosynthesis in land plants. | Jinghua Lu Chunyang Pan Xin Li Zejun Huang Jinshuai Shu Xiaoxuan Wang Xiaoxiao Lu Feng Pan Junling Hu Hui Zhang Wenyue Su Min Zhang Yongchen Du Lei Liu Yanmei Guo Junming Li | 2021 | Horticulture Research2021，8，1： | 1 |
| 6 | Bioresponsive cisplatin crosslinked albumin hydrogel served for efficient cancer combination therapy显示文摘Combination therapy is one of the potential strategies for tackling complicated tumor treatments like drug resistance.In this work,we have generated a therapeutic cisplatin-crosslinked albumin hydrogel(BC-Gel)that allows the local release of L-Buthionine-sulfoximine(BSO),cisplatin,and glucose oxidase(GOx)with distinct release kinetics.The BC-Gel with favorable biostimuli degradability and injectability could release therapeutic agents in a programmed manner within the tumor microenvironment(TME).The preferentially released BSO significantly suppressed the glutathione(GSH)-related cisplatin resistance and sensitized the tumor cells to cisplatin by inhibiting theγ-glutamylcysteine synthetase.Meanwhile,cisplatin achieved a sequential release and long-term treatment following the bioresponsive gel degradation under the combined action of chloride ions(Cl−)and proteinase in the body.In addition,the overproduced H_(2)O_(2)of GOx-catalyzed glucose oxidation accelerated the depletion of existed GSH within cells and further weakened the cisplatin resistance,achieving enhanced tumor treatment together with a strong cell-killing effect.The above sequential drug release strategy based on the dual GSH depletion effect breaks the balance of the GSH-mediated redox TME and enhances the sensitivity of A549 cells to cisplatin forcefully,and provides a promising way for temporal control of drug release as well as efficient cancer combination therapy. | An Yan Zherui Zhang Jiamei Gu Xiaoran Ding Yongchen Chen Jingjing Du Shu Wei Hongcheng Sun Jiayun Xu Shuangjiang Yu Junqiu Liu | 2023 | Nano Research2023，16，2： | 1 |
| 7 | Welcome to the Second Asian Horticultural Congress 2016显示文摘The Second Asian Horticultural Congress will be opened in Chengdu,Sichuan of China during September 26 to 28,2016.This time,it will be co-hosted by International Society for Horticultural Science,Chinese Society for Horticultural Science,Korean Society for Horticultural Science and Japanese Society for Horticultural Science,and co-organized by | Yongchen Du | 2015 | Horticultural Plant Journal2015，1，3： | 0 |
| 8 | Identificatio of Quantitative Trait Loci for Fruit Weight, Soluble Solids Content, and Plant Morphology Using an Introgression Line Population of Solanum pennellii in a Fresh Market Tomato Inbred Line显示文摘Introgression lines are convenient populations for the identification fine-mapping and functional analysis of genes that are responsible for variations in traits, particularly quantitative trait genes. An introgression line population of Solanum pennellii LA0716 in a fresh market tomato inbred line 1052 was developed by our group. This population was composed of 214 lines. In the present study, fi e quantitative trait loci(QTLs)for fruit weight, two QTLs for soluble solids content(SSC), three QTLs for plant height, and one QTL for leaf size were identifie using this introgression line population. Among these, fw3 a and fw4 a for fruit weight, ssc7 a for SSC, h4t2 a, h4t3 a, and h4t7 a for plant height, and lz12 a for leaf size were determined to be novel loci. These results serve as a foundation for fine-mappin and functional analysis of genes underlying these QTLs. | ZHOU Hui WANG Xiaoxuan HUANG Zejun GAO Jianchang GUO Yanmei DU Yongchen HU Hong | 2016 | Horticultural Plant Journal2016，2，1： | 0 |
| 9 | Welcome to the Second Asian Horticultural Congress 2016显示文摘The Second Asian Horticultural Congress will be opened in Chengdu,Sichuan of China during September 26 to 28,2016.This time,it will be co-hosted by International Society for Horticultural Science,Chinese Society for Horticultural Science,Korean Society for Horticultural Science and Japanese | Yongchen Du | 2015 | Horticultural Plant Journal2015，1，2： | 0 |
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