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| 1 | Expression of ipt gene driven by tomato fruit specific promoter and its effects on fruit development of tomato显示文摘Fruit specific promoter (2A12) from Lycopersi-com esculentum and cDNA of isopentenyl-transferase (ipt) from Ti plasmid of Agrobacterium tumerfaciens C58 were cloned by PCR procedure respectively. Two plant expression vectors with 2A12/gus or 2A12/ipt were respectively constructed. These two chimeric genes were transferred into tomato by Agrobacterium mediated procedure. The results of Southern hybridization showed that the fusion genes had been integrated into tomatoes. The result of gus histochemi-cal staining showed that 2A12 had high fruit specific expressive capability in transgenic tomato. The ipt expression resulted in accumulation of high level of cytokinins (CTKs) in fruit lead to developmental changes in fruits and seeds. The fruit of ipt transformed tomato had the hyperplastic placenta with very few seeds or even seedless. The shelf life of transgenic fruits elongated for 1-2 weeks. The ratio of fruit set, the dry weight of fruit and the crude protein content in fruit were increased, while | Zichao Mao Qiuju Yu Wei Zhen Junyi Guo Yuanlei Hu Yin Gao Zhongping Lin | 2002 | Chinese Science Bulletin2002，47，11： | 6 |
| 2 | Boosting the photodynamic therapy efficiency with a mitochondria-targeted nanophotosensitizer显示文摘Photodynamic therapy (PDT) is an attractive clinical technique for cancer treatment. However, the poor solubility and weak cellular internalization of the molecule-photosensitizers, as well as the exceedingly short lifetime and limited diffusion distance of the generated reactive oxygen species (ROS) in cytoplasm directly restricted the therapeutic efficiency of conventional PDT. In this study, we proposed a facile strategy for improving PDT of cancer based on a mitochondria-targeted nanophotosensitizer. The molecule-photosensitizer chlorin e6 was covalently attached on the internal and external surfaces of the mesoporous silica nanoparticles. Then, the triphenylphosphonium was anchored on the nanoparticles for selectively targeting mitochondria. When irradiated with laser, the nanophotosensitizer can generate a large amount of ROS in mitochondria, thus causing the mitochondrial dysfunction and irreversible cell apoptosis. In vitro and in vivo studies demonstrated that the nanophotosensitizer could boost the treatment efficiency against cancer cells and xenograft tumor models. | Limin Yang Peng Gao Yuanlei Huang Xiao Lu Qian Chang Wei Pan Na Li Bo Tang | 2019 | Chinese Chemical Letters2019，30，6： | 4 |
| 3 | Cloning and expression pattern of a dehydrin-like BDNI gene from drought-tolerant Boea crassifolia Hemsl.显示文摘A 500-bp cDNA fragment was amplified via RT-PCR from drought-induced total RNA of the drought-tolerant B. crassifolia Hemsl. using primers based on the sequence of published dehydrin conserved region. By using 5’ RACE, full-length coding region (1 148 bp) of BDN1 gene was produced. It is a new member of the dehydrin gene family. Southern analysis indicated that BDN1 is present in the 6. crassifolia genome as a single-copy gene. Northern analysis revealed that its expression is inducible by drought and cold stresses as well as ABA application. | ZHAO Huiwu, LIU Han, YU Haiyuan, HU Yuanlei, GAO Yin, LI Zhenyu & LIN ZhongpingNational Laboratory of Protein Engineering and Plant Genetic Engineering , Peking University, Beijing 100871, China Botanical Institute, Chinese Academy of Sciences, Beijing 100093, China Correspondence should be addressed to Lin Zhongping | 2000 | Chinese Science Bulletin2000，45，22： | 3 |
| 4 | Enhanced late blight resistance of transgenic potato expressing glucose oxidase under the control of pathogen-inducible promoter显示文摘To engineer crop disease resistance by utilizing natural defense mechanism that was expressed in the incompatible host-pathogen interactions is expected to result in a durable and broad-spectrum resistance. In order to prove this viewpoint, we amplified the coding region of the glucose oxidase (GO) gene from Aspergillus nigervia PCR and fused it to the pathogen-inducible promoter, Prp1-1. The chimeric gene was cloned into a plant expression vector and conjugated into Agrobacterium. Twenty-three transgenic potato plants were obtained by Agrobacterium-mediated transformation. The integration of GO gene was confirmed by Southern hybridization and the GO gene expression was identified with Kl-starch color reaction. Phytophthora infestans inoculation revealed that the expression of the chimeric transgene was induced by pathogen infection. Most of the transgenic plants exhibited various degrees of enhanced disease resistance. Four of them had lesion sizes reduced to less than half of the non-transgenic | Wei Zhen Xi Chen Haobo Liang Yuanlei Hu Yin Gao Zhongping Lin | 2000 | Chinese Science Bulletin2000，45，21： | 2 |
| 5 | High Content of Resveratrol in Lettuce Transformed with a Stilbene Synthase Gene of Parthenocissus henryana显示文摘 | Liu Shujun Hu Yuanlei Wang Xiaoli | 2006 | Journal of Agricultural and Food Chemistry2006，54，21： | 1 |
| 6 | Expression profile of a PAL gene from Astragalus membranaceus var. Mongholicus and its crucial role in flux into flavonoid biosynthesis显示文摘 | Rongrong Liu Shaohua Xu Jialin Li Yuanlei Hu Zhongping Lin | 2006 | Plant Cell Reports2006，，7： | 1 |
| 7 | Inducible Excision of Selectable Marker Gene from Transgenic Plants by the Cre/lox Site-specific Recombination System显示文摘 | Yong Wang Bojun Chen Yuanlei Hu Jingfu Li Zhongping Lin | 2005 | Transgenic Research2005，，5： | 1 |
| 8 | Bone mar- row stromal cells enhance the angiogenesis in ischaemic cortex after stroke : involvement of notch signalling 显示文摘 | Guo Fci Lv Shigang Lou Yuanlei | 2012 | Cell Biology International2012，36，11： | 1 |
| 9 | Efficient transformation of the medicinal mushroomGanoderma lucidum显示文摘 | Li Sun Huaqing Cai Weihong Xu Yuanlei Hu Yin Gao Zhongping Lin | 2001 | Plant Molecular Biology Reporter2001，，： | 1 |
| 10 | Transgenic tall rescue containing the Agrobacterium tumefaciens lpt gene shows enhanced cold tolerance显示文摘 | HU Yuanlei JIA Weilong WANG Jundan | 2005 | Plant Cell Reports2005，23，： | 1 |
| 11 | A phytocyanin-related early nodulin-like gene, BcBCP1 , cloned from Boea crassifolia enhances osmotic tolerance in transgenic tobacco显示文摘 | Hanying Wu Ye Shen Yuanlei Hu Sijun Tan Zhongping Lin | 2011 | Journal of Plant Physiology2011，，9： | 1 |
| 12 | Sense and antisense expression of plasma membrane aquaporin BnPIP1 from Brassica napus into tobacco and its effects on plant drought resistance 显示文摘 | YU Qiuju HU Yuanlei LI Jingfu et O1 | 2005 | Plant Sci2005，169，4： | 1 |
| 13 | High cintent of resveratrol in lettuce tranceformed with a stilbene syntyase gene of parthenocissus henryana显示文摘 | Liu Shujun Hu Yuanlei Wang xiaoli | 2006 | Journal of Agricultural and Food Chemistry2006，54，21： | 1 |
| 14 | Sense and anti sense expression of plasma membrane aquaporin BnPIP1 from Brassica napus in tobacco and its effects on plant drought resistance显示文摘 | Yu Qiuju Hu Yuanlei Li Jingfu | 2005 | Plant Science2005，，169： | 1 |
| 15 | A big-data approach for investigating destination image gap in Sanya City: When will the online and the offline goes parted?显示文摘Tourism destination images in terms of the gaps between the projected and perceived images are of great significance in the development of destinations.Additionally,the use of big-data in tourism studies remains under-utilized despite the boom in big-data applications and the increasing number of electronic User Generated Contents(UGC).Aiming to take advantage of tourism UGC to fully understand the destination image gap between official promotion materials and tourist perception of Sanya City in China,this study innovatively employed a big-data analysis technique,Tourism Sentiment Evaluation(TSE)model and proposed a new analysis framework integrating the“cognitive-affective”model with the gpp analysis of projected and perceived destination image to explore the destination image gap of Sanya It is found that Sanya's perceptive destination image is overall consistent with its official positioning;however,there also exist image gaps between the two groups in terms of the impact of festival events and tourists'attitude towards core scenic spots amongst others.This study's findings are discussed in light of their methodological,theoretical,and practical implications for destination positioning,marketing,and management. | Lingkun Meng Yi Liu Yuanlei Wang Xiaojuan Li | 2021 | Regional Sustainability2021，2，1： | 0 |
| 16 | Oral immunogenicity of potato-derived antigens to Mycobacterium tuberculosis in mice显示文摘为病毒、细菌的抗原的表示的向量逐渐地为试验性的口头的疫苗的生产和交货作为其他的方法论被测试了的转基因的植物的新奇使用。这里,我们检验了包括从 Mycobacterium 肺结核编码免疫者主导的抗原的四基因的联合做植物的疫苗的 immunogenicity。与野类型和另外的控制组相比,与联合做植物的疫苗对待的老鼠显著地响应抗原特定的 CD4+ 和 CD8+T 房间回答和免疫球蛋白(Ig ) 的所有四蛋白质,和高水平显示出 interferon-2 和 interleukin-2 生产的高水平 G 和 IgA titers。这些结果建议联合做植物的疫苗能对 M 导致 immunogenicity。通过更强壮的联系 Th1 的有免疫力的回答的正式就职的肺结核。这是对肺结核 priming 的一支口头上地交付的联合做植物的疫苗的第一份报告抗原特定的 Th1 回答,包括的全面效果 mucosal 和全身的有免疫力的回答。 | Yi Zhang Suting Chen Jiayun Li Yuan Liu Yuanlei Hu Hong Cai | 2012 | Acta Biochimica et Biophysica Sinica2012，44，10： | 0 |
| 17 | Co-transformation to tobacco of Cre/lox site-specific recombination system and its precise recombination显示文摘For the temporally and spatially regulated expression of the barnase gene in plant, two kinds of plasmids with ere gene and its directly repeat recognition sites lox from bacteriophage P1 were constructed and co-transformed into tobacco by agrobacterium mediated procedure. The transgenic plants were conformed by PCR analysis. The blocking fragment between the two lox directly repeat sites was excised by Cre protein in the transgenic plant genome. Cloning and seguencing the DNA fragment from the co-transformed plant DNA showed that the precise DNA excision occurred in transgenic tobacco genome directed by Cre//ox site-specif ic recombination . | Lei Li Songmei Liu Yuanlei Hu Zhongping Lin | 2000 | Chinese Science Bulletin2000，45，6： | 0 |
| 18 | Increase of sulphur-containing amino acids in transgenic potato with 10 ku zein gene from maize显示文摘The 10 ku zein gene of maize was under control of patatin class I promoter of potato and transferred into potato genome by the leaf-disc method. The expression of 10 ku zein was determined in the tuber of transgenic plants by RT-PCR. Furthermore, the sulphur-containing amino acids in transgenic tuber increase remarkably. | LI Lei LIU Songmei HU Yuanlei ZHAO Weiping LIN Zhongping | 2001 | Chinese Science Bulletin2001，46，6： | 0 |
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