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| 1 | Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells显示文摘RNase III DROSHA is upregulated in multiple cancers and contributes to tumor progression by hitherto unclear mechanisms.Here,we demonstrate that DROSHA interacts withβ-Catenin to transactivate STC1 in an RNA cleavage-independent manner,contributing to breast cancer stem-like cell(BCSC)properties.DROSHA mRNA stability is enhanced by N6-methyladenosine(m^(6)A)modification which is activated by AURKA in BCSCs.AURKA stabilizes METTL14 by inhibiting its ubiquitylation and degradation to promote DROSHA mRNA methylation.Moreover,binding of AURKA to DROSHA transcript further strengthens the binding of the m^(6)A reader IGF2BP2 to stabilize m^(6)A-modified DROSHA.In addition,wild-type DROSHA,but not an m^(6)A methylation-deficient mutant,enhances BCSC stemness maintenance,while inhibition of DROSHA m^(6)A modification attenuates BCSC traits.Our study unveils the AURKA-induced oncogenic m^(6)A modification as a key regulator of DROSHA in breast cancer and identifies a novel DROSHA transcriptional function in promoting the BCSC phenotype. | Fei Peng Jie Xu Bai Cui Qilan Liang Sai Zeng Bin He Hong Zou Manman Li Huan Zhao Yuting Meng Jin Chen Bing Liu Shasha Lv Peng Chu Fan An Zifeng Wang Junxiu Huang Yajing Zhan Yuwei Liao Jinxin Lu Lingzhi Xu Jin Zhang Zhaolin Su Zhiguang Li Fangjun Wang Eric W-FLam Quentin Liu | 2021 | Cell Research2021，31，3： | 9 |
| 2 | Guiding T lymphopoiesis from pluripotent stem cells by defined transcription factors显示文摘Achievement of immunocompetent and therapeutic T lymphopoiesis from pluripotent stem cells(PSCs)is a central aim in T cell regenerative medicine.To date,preferentially reconstituting T lymphopoiesis in vivo from PSCs remains a practical challenge.Here we documented that synergistic and transient expression of Runx1 and Hoxa9 restricted in the time window of endothelial-to-hematopoietic transition and hematopoietic maturation stages in a PSC differentiation scheme(iR9-PSC)in vitro induced preferential generation of engraftable hematopoietic progenitors capable of homing to thymus and developing into mature T cells in primary and secondary immunodeficient recipients.Single-cell transcriptome and functional analyses illustrated the cellular trajectory of T lineage induction from PSCs,unveiling the T-lineage specification determined at as early as hemogenic endothelial cell stage and identifying the bona fide pre-thymic progenitors.The induced T cells distributed normally in central and peripheral lymphoid organs and exhibited abundant TCRαβrepertoire.The regenerative T lymphopoiesis restored immune surveillance in immunodeficient mice.Furthermore,gene-edited iR9-PSCs produced tumor-specific T cells in vivo that effectively eradicated tumor cells.This study provides insight into universal generation of functional and therapeutic T cells from the unlimited and editable PSC source. | Rongqun Guo Fangxiao Hu Qitong Weng Cui Lv Hongling Wu Lijuan Liu Zongcheng Li Yang Zeng Zhijie Bai Mengyun Zhang Yuting Liu Xiaofei Liu Chengxiang Xia Tongjie Wang Peiqing Zhou Kaitao Wang Yong Dong Yuxuan Luo Xiangzhong Zhang Yuxian Guan Yang Geng Juan Du Yangqiu Li Yu Lan Jiekai Chen Bing Liu Jinyong Wang | 2020 | Cell Research2020，30，1： | 7 |
| 3 | The integration of transcriptomic and transgenic analyses reveals the involvement of the SA response pathway in the defense of chrysanthemum against the necrotrophic fungus Alternaria sp.显示文摘Chrysanthemum morifolium cv.‘Huaihuang’has ornamental,edible,medicinal,and tea product uses.However,its field growth,yield,and quality are negatively affected by black spot disease caused by Alternaria sp.(Strain:HQJH10092301;GenBank accession number:KF688111).In this study,we transcriptionally and transgenically characterized a new cultivar,‘Huaiju 2#’(Henan Traditional Chinese Medicine Plant Cultivar identification number:2016002),which was bred from‘Huaihuang’and shows resistance to Alternaria sp.Numerous‘Huaiju 2#’plants were inoculated with Alternaria sp.for three or five days.Metabolic analysis showed increases in both salicylic acid(SA)and jasmonic acid(JA)in infected plants compared to the control.Protein activity analysis also revealed a significant increase in defense enzyme activities in infected plants.RNA-Seq of plants infected for 3 or 5 days produced a total of 58.6 GB of clean reads.Among these reads,16,550 and 13,559 differentially expressed genes(DEGs)were identified in Cm_3 dpi(sample from 3 days post-inoculation labeled as Cm_3 dpi)and Cm_5 dpi(sample from 5 days post-inoculation labeled as Cm_5 dpi),respectively,compared with their controls(Cm_0 d:a mixture samples from 0 d(before inoculation)and those treated with sterile distilled water at 3 dpi and 5 dpi).Gene annotation and cluster analysis of the DEGs revealed a variety of defense responses to Alternaria sp.infection,which were characterized by increases in resistance(R)proteins and the reactive oxygen species(ROS),Ca2+,mitogen-activated protein kinase(MAPK),and JA signaling pathways.In particular,SA signaling was highly responsive to Alternaria sp.infection.The qPCR analysis of 12 DEG candidates supported their differential expression characterized by using the RNA-Seq data.One candidate was CmNPR1(nonexpressor of pathogenesis-related gene 1),an important positive regulator of SA in systemic acquired resistance(SAR).Overexpression of CmNPR1 in‘Huaiju 2#’increased the resistance of transgenic plants to black spot.These findings indicate that the SA response pathway is likely involved in the defense of‘Huaiju 2#’against Alternaria sp.pathogens. | Xiting Zhao Lingyu Song Liwei Jiang Yuting Zhu Qinghui Gao Dandan Wang Jing Xie Meng Lv Ping Liu Mingjun Li | 2020 | Horticulture Research2020，7，1： | 3 |
| 4 | DDIT4 mediates the proliferation-promotive effect of IL-34 in human monocytic leukemia cells显示文摘Interleukin 34(IL-34)is a cytokine that shares the receptor with colony-stimulating factor 1(CSF-1).IL-34 is involved in a broad range of pathologic processes including cancer.We previously demonstrated that IL-34 promoted the proliferation and colony formation of human acute monocytic leukemia(AMoL)cells.However,the mechanism has not been elucidated.Here,by analyzing the gene profiles of Molm13 and THP1 cells overexpressing IL-34(Molm13-IL-34 and THP1-IL-34),upregulation of the DNA damageinducible transcript 4(DDIT4)was detected in both series.Knockdown of DDIT4 effectively inhibited the proliferation,promoted apoptosis and colony formation in Molm13-IL-34 and THP1-IL-34 cells.Our results suggest that DDIT4 mediates the proliferationpromotive effect of IL-34 whereas does not mediate the promotive effect of IL-34 on colony formation in AMoL cells. | Xiaoqian Lv Yuting Hu Lina Wang Dongyue Zhang Hao Wang Yibo Dai Xiaoxi Cui Guoguang Zheng | 2021 | Blood Science2021，3，2： | 2 |
| 5 | Earthquake detection in the Jiangsu region, China using graphics-processing-unit-based Match & Locate and rapid earthquake association and location显示文摘Earthquake detection and location are essential in earthquake studies,which generally consists of two main classes:waveform-based and pick-based methods.To evaluate the ability of two different methods,a graphicsprocessing-unit-based Match&Locate(GPU-M&L)method and a rapid earthquake association and location(REAL)method are applied to continuous seismic data recorded by 24 digital seismic stations from Jiangsu Seismic Network during 2013 for comparison.GPU-M&L is one of waveform-based methods by waveform cross-correlations while REAL is one of pick-based method to associate arrivals of different seismic phases and locate events through counting the number of P and S picks and travel time residuals.Twenty-six templates are selected from the Jiangsu Seismic Network local catalog by using the GPU-M&L.The number of newly detected and located events is about 2.8 times more than those listed in the local catalog.We both utilize a deep-neural-network-based arrival-time picking method called PhaseNet and a shortterm/long-term average(STA/LTA)trigger algorithm for seismic phase detection and picking by applying the REAL.We then refine seismic locations using a least-squares location method(VELEST)and a high-precision relative location method(hypoDD).By applying STA/LTA and PhaseNet,1006 and 1893 events are associated and located,respectively.The newly detected events are mainly clustered and show steeply dipping fault planes.By analyzing the performance of these methods based on long-term continuous seismic data,the detected catalogs by the GPU-M&L and REAL show that the magnitudes of completeness are 1.4 and 0.8,respectively,which are smaller than 2.6 given by the local catalog.Although REAL provides improvement compared with GPU-M&L,REAL is highly dependent on phase detection and picking which is strongly affected by signal-noise ratio(SNR).Stations at southeast of the study region with low SNR may lead to few detections in the same area. | Yafen Huang Shengzhong Zhang Yuejun Lv Yanzhen Li Yuting Zhang Min Liu | 2020 | Earthquake Science2020，33，1： | 2 |
| 6 | Flexible Hf_(0.5)Zr_(0.5)O_(2)ferroelectric thin films on polyimide with improved ferroelectricity and high flexibility显示文摘Flexible memory devices are promising for information storage and data processing applications in portable,wearable,and smart electronics operating under curved conditions.In this work,we realized high-performance flexible ferroelectric capacitors based on Hf_(0.5)Zr_(0.5)O_(2)(HZO)thin film by depositing a buffer layer of Al_(2)O_(3)on polyimide(PI)substrates using atomic layer deposition(ALD).The flexible ferroelectric HZO films exhibit high remnant polarization(Pr)of 21μC/cm^(2).Furthermore,deterioration of polarization,retention,and endurance performance was not observed even at a bending radius of 2 mm after 5,000 bending cycles.This work marks a critical step in the development of high-performance flexible HfO_(2)-based ferroelectric memories for next-generation wearable electronic devices. | Yuting Chen Yang Yang Peng Yuan Pengfei Jiang Yuan Wang Yannan Xu Shuxian Lv Yaxin Ding Zhiwei Dang Zhaomeng Gao Tiancheng Gong Yan Wang Qing Luo | 2022 | Nano Research2022，15，4： | 1 |
| 7 | 一个中央杏仁核—无名质神经环路编码厌恶增强信号(英文)显示文摘文章简介Aversive stimuli can impact motivation and support associative learning as reinforcers.However,the neural circuitry underlying the processing of aversive reinforcers has not been elucidated.Here,we report | Yuting Cui Guanghui Lv Sen Jin Jie Peng Jing Yuan Xiaobin He Hui Gong Fuqiang Xu 许彤辉 李浩洪 | 2018 | 科学新闻2018，0，4： | 0 |
| 8 | VOC degradation by microwave-induced metal discharge and thermal destruction:a comparative study显示文摘The effective treatment of volatile organic compounds(VOCs)is essential because of their direct effects on air pollution and human health.This paper introduces microwave-induced metal discharge as a highly effective and byproduct value-added approach to degrade high-concentration toluene as a model VOC.The effect of the factors that influence the discharge intensity,including the metal type(Fe,Cu,Ni,Zn)and amount,was investigated.Degradation efficiency of toluene can reach 79.76% under optimal discharge condition.In addition,the discharge method was compared with traditional thermal destruction at 700℃,900℃ and 1100℃.The gaseous and liquid cracking products of toluene produced by the microwave-induced metal discharge method were almost similar to those obtained under thermal destruction at 900℃;however,the solid-phase discharge products were nanoparticles that demonstrated good graphitization,while the thermal destruction products were amorphous microparticles.This work offers an effective and flexible way to degrade high-concentration VOCs and also provides an application reference for biomass tar cracking and removal of other organic pollutants. | Yuting Lv Yuli Zhou Wenlong Wang Jing Sun Zhanlong Song Ke Wang | 2019 | Waste Disposal and Sustainable Energy2019，1，4： | 0 |
| 9 | Multi-temporal urban semantic understanding based on GF-2 remote sensing imagery:from tri-temporal datasets to multi-task mapping显示文摘High resolution satellite images are becoming increasingly available for urban multi-temporal semantic understanding.However,few datasets can be used for land-use/land-cover(LULC)classification,binary change detection(BCD)and semantic change detection(SCD)simultaneously because classification datasets always have one time phase and BCD datasets focus only on the changed location,ignoring the changed classes.Public SCD datasets are rare but much needed.To solve the above problems,a tri-temporal SCD dataset made up of Gaofen-2(GF-2)remote sensing imagery(with 11 LULC classes and 60 change directions)was built in this study,namely,the Wuhan Urban Semantic Understanding(WUSU)dataset.Popular deep learning based methods for LULC classification,BCD and SCD are tested to verify the reliability of WUSU.A Siamese-based multi-task joint framework with a multi-task joint loss(MJ loss)named ChangeMJ is proposed to restore the object boundaries and obtains the best results in LULC classification,BCD and SCD,compared to the state-of-the-art(SOTA)methods.Finally,a large spatial-scale mapping for Wuhan central urban area is carried out to verify that the WUsU dataset and the ChangeMJ framework have good application values. | Sunan Shi Yanfei Zhong Yinhe Liu Jue Wang Yuting Wan Ji Zhao Pengyuan Lv Liangpei Zhang Deren Li | 2023 | International Journal of Digital Earth2023，16，1： | 0 |
| 10 | Microstructures and Mechanical Properties of the TiC/CM247LC Nickel-Based Composite Fabricated by Selective Laser Melting:Effect of Heat Treatment显示文摘In this study,TiC/CM247LC nickel-based composite was successfully prepared by selective laser melting,then was heat treated at a solid solution temperature of 1260℃and different aging temperature of 840℃,870℃,900℃and 930℃respectively.Effects of aging temperatures on the microstructures and mechanical properties were systematically studied.The results show that the microstructures of all the heat treated samples are composed ofγmatrix,carbides andγ′phase.Theγgrains remain a columnar shape after treatments,but the size ofγ′phase grows up gradually with the increasing aging temperature.The composite treated at an aging temperature of 870℃exhibits the best mechanical properties with the tensile strength of 1073 MPa,yield strength of 1004 MPa and elongation of 7.57%.The plastic deformation and strengthening mechanisms of heat treated composite were systematically investigated. | Yuting Lv Yaojie Liu Zhe Zhang Qiang Zhang Hongyao Yu Rui Wang Guangbao Sun Guijiang Wei | 2023 | Acta Metallurgica Sinica(English Letters)2023，36，12： | 0 |
| 11 | Freely switching between ferroelectric and resistive switching in Hf_(0.5)Zr_(0.5)O_(2) films and its application on high accuracy on-chip deep neural networks显示文摘The Hf_(0.5)Zr_(0.5)O_(2)(HZO)-based ferroelectric field-effect transistor(Fe FET) synapse is a promising candidate for at-scale deep neural network(DNN) applications, because of its high symmetry, great accuracy and fast operation speed. However, the degradation of the remanent polarization(Pr) over time caused by the depolarization field has not been effectively resolved, greatly affecting the accuracy of the trained DNN. In this study, we demonstrate a ferroelectric(FE)-resistive switching(RS) switchable synapse using the FE mode for high-speed weight training and the RS mode for stable weight storage to overcome accuracy degradation. The FE-RS hybrid characteristic is accomplished by an HZO-based metal-ferroelectricmetal(MFM) capacitor with asymmetric electrodes, and the best FE endurance, as well as the most reliable RS behavior, is demonstrated by testing several electrodes materials. High memory windows are achieved in both FE and RS modes. Through this design, excellent accuracy is maintained over time, as verified by network simulation. | Pengfei JIANG Kunran XU Jie YU Yannan XU Peng YUAN Yuan WANG Yuting CHEN Yaxin DING Shuxian LV Zhiwei DANG Tiancheng GONG Yang YANG Yan WANG Qing LUO | 2023 | Science China(Information Sciences)2023，66，2： | 0 |
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