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| 1 | Key residues of the receptor binding motif in the spike protein of SARS-CoV-2 that interact with ACE2 and neutralizing antibodies显示文摘Coronavirus disease 2019(COVID-19),caused by the novel human coronavirus SARS-CoV-2,is currently a major threat to public health worldwide.The viral spike protein binds the host receptor angiotensin-converting enzyme 2(ACE2)via the receptor-binding domain(RBD),and thus is believed to be a major target to block viral entry.Both SARS-CoV-2 and SARS-CoV share this mechanism.Here we functionally analyzed the key amino acid residues located within receptor binding motif of RBD that may interact with human ACE2 and available neutralizing antibodies.The in vivo experiments showed that immunization with either the SARS-CoV RBD or SARS-CoV-2 RBD was able to induce strong clade-specific neutralizing antibodies in mice;however,the cross-neutralizing activity was much weaker,indicating that there are distinct antigenic features in the RBDs of the two viruses.This finding was confirmed with the available neutralizing monoclonal antibodies against SARS-CoV or SARS-CoV-2.It is worth noting that a newly developed SARS-CoV-2 human antibody,HA001,was able to neutralize SARS-CoV-2,but failed to recognize SARS-CoV.Moreover,the potential epitope residues of HA001 were identified as A475 and F486 in the SARS-CoV-2 RBD,representing new binding sites for neutralizing antibodies.Overall,our study has revealed the presence of different key epitopes between SARS-CoV and SARSCoV-2,which indicates the necessity to develop new prophylactic vaccine and antibody drugs for specific control of the COVID-19 pandemic although the available agents obtained from the SARS-CoV study are unneglectable. | Chunyan Yi Xiaoyu Sun Jing Ye Longfei Ding Meiqin Liu Zhuo Yang Xiao Lu Yaguang Zhang Liyang Ma Wangpeng Gu Aidong Qu Jianqing Xu Zhengli Shi Zhiyang Ling Bing Sun | 2020 | Cellular & Molecular Immunology2020，17，6： | 13 |
| 2 | Involvement of C2H2 zinc finger proteins in the regulation of epidermal cell fate determination in Arabidopsis显示文摘Cell fate determination is a basic developmental process during the growth of multicellular organisms.Trichomes and root hairs of Arabidopsis are both readily accessible structures originating from the epidermal cells of the aerial tissues and roots respectively, and they serve as excellent models for understanding the molecular mechanisms controlling cell fate determination and cell morphogenesis. The regulation of trichome and root hair formationis a complex program that consists of the integration of hormonal signals with a large number of transcriptional factors, including MYB and b HLH transcriptional factors.Studies during recent years have uncovered an important role of C2H2 type zinc finger proteins in the regulation of epidermal cell fate determination. Here in this minireview we briefly summarize the involvement of C2H2 zinc finger proteins in the control of trichome and root hair formation in Arabidopsis. | An Yan Minjie Wu Yongqin Zhao Aidong Zhang Bohan Liu John Schiefelbein Yinbo Gan | 2014 | Journal of Integrative Plant Biology2014，56，12： | 7 |
| 3 | Construction and Expression of a Single Chain Antibody Mimicing Human Ovarian Cancer Antigen CA125显示文摘为癌症的有免疫力的治疗的一个概念包含抗原的正式就职模仿抗体对肿瘤房间触发有免疫力的反应。对为肿瘤抗原特定的抗体的抗原绑定地点指导的 Anti-idiotypic 抗体可以机能上地并且甚至在结构上模仿抗原并且导致 anti-anti-idiotypic 免疫者反应。Monoclonal 抗体 WJ02 是如此的 anti-idiotypic 抗体之一,它包含 CA125 的内部图象。以便改进 mAb WJ02 的 immunospecificity,我们在 Vl-linker-Vh 取向构造了 mAb WJ02 的单个链。scFv-WJ02 能在 recombinant Pichia pastoris 系统被表示并且藏匿。有 30 kD 的分子的重量的分泌 scFv 蛋白质保留了 mAb WJ02 的生物活动,它被直接有约束力的试金和抑制实验证明。我们的结果显示 scFv-WJ02 能对卵巢的癌症为 idiotypic 治疗被用作一个可能的工具,它可能提高消除 mAb WJ02 导致的 nonspecific 回答的可能性。 | Aidong Li Zheng Li Yinghong Wang Yongming Zhang Jie Ma | 2006 | Cellular & Molecular Immunology2006，3，1： | 5 |
| 4 | Corridor use by Asian elephants显示文摘There are 18 km of Kunming–Bangkok Highway passing through the Mengyang Nature Reserve of Xishuangbanna National Nature Reserve in Yunnan Province,China.From September 2005 to September 2006 the impact of this highway on movement of wild Asian elephants between the eastern and western part of the nature reserve was studied using track transecting,rural surveys and direct monitoring.Our results showed that the number of cross-road corridors used by Asian elephants diminished from 28 to 23 following the construction of the highway.In some areas,the elephant activity diminished or even disappeared,which indicated a change in their home ranges.The utilization rate of artificial corridors was 44%.We also found that elephants prefered artificial corridors that were placed along their original corridors.During the research,wild elephants revealed their adaptation to the highway.They were found walking across the highway road surface many times and for different reasons.We suggest that the highway management bureau should revise their management strategies to mitigate the potential risks caused by elephants on the road for the safety of the public and to protect this endangered species from harm.It is also very important to protect and maintain current Asian elephants corridors in this region. | Wenjing PAN Liu LIN Aidong LUO Li ZHANG | 2009 | Integrative Zoology2009，4，2： | 4 |
| 5 | 基于多视图融合的蛋白质功能模块检测方法显示文摘结合多种生物数据分析蛋白质相互作用网络(Protein-Protein Interaction Network,PPIN)中的功能模块结构,是目前蛋白质功能计算分析领域亟待解决的难题之一.本文提出了一种基于聚合非负矩阵分解(Collective Non-negative Matrix Factorization,CoNMF)的多视图一致性功能模块检测方法,该方法同时逼近多视图数据,寻找统一的最优解达到对原多数据的最优近似.根据该统一解得到功能模块关系,同时该方法能够找到可重叠性的功能模块.实验结果显示本文所提出算法通过融合基因本体、基因表达谱与PPIN数据,在模块检测准确度上有一定提高,检测出的蛋白质功能模块具有真实生物意义. | 张媛 贾克斌 ZHANG Aidong | 2014 | 电子学报2014，42，12： | 3 |
| 6 | Clustering PPI Data Based on Ant Colony Optimization Algorithm显示文摘 | LEI Xiujuan WU Shuang GE Liang ZHANG Aidong | 2013 | Chinese Journal of Electronics2013，22，1： | 3 |
| 7 | Safety and efficacy of meplazumab in healthy volunteers and COVID-19 patients: a randomized phase 1 and an exploratory phase 2 trial显示文摘Recent evidence suggests that CD147 serves as a novel receptor for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection.Blocking CD147 via anti-CD147 antibody could suppress the in vitro SARS-CoV-2 replication.Meplazumab is a humanized anti-CD147 IgG_(2) monoclonal antibody,which may effectively prevent SARS-CoV-2 infection in coronavirus disease 2019(COVID-19)patients.Here,we conducted a randomized,double-blinded,placebo-controlled phase 1 trial to evaluate the safety,tolerability,and pharmacokinetics of meplazumab in healthy subjects,and an open-labeled,concurrent controlled add-on exploratory phase 2 study to determine the efficacy in COVID-19 patients.In phase 1 study,59 subjects were enrolled and assigned to eight cohorts,and no serious treatment-emergent adverse event(TEAE)or TEAE grade≥3 was observed.The serum and peripheral blood Cmax and area under the curve showed non-linear pharmacokinetic characteristics.No obvious relation between the incidence or titer of positive anti-drug antibody and dosage was observed in each cohort.The biodistribution study indicated that meplazumab reached lung tissue and maintained>14 days stable with the lung tissue/cardiac blood-pool ratio ranging from 0.41 to 0.32.In the exploratory phase 2 study,17 COVID-19 patients were enrolled,and 11 hospitalized patients were involved as concurrent control.The meplazumab treatment significantly improved the discharged(P=0.005)and case severity(P=0.021),and reduced the time to virus negative(P=0.045)in comparison to the control group.These results show a sound safety and tolerance of meplazumab in healthy volunteers and suggest that meplazumab could accelerate the recovery of patients from COVID-19 pneumonia with a favorable safety profile. | Huijie Bian Zhao-Hui Zheng Ding Wei Aidong Wen Zheng Zhang Jian-Qi Lian Wen-Zhen Kang Chun-Qiu Hao Jing Wang Rong-Hua Xie Ke Dong Jie-Lai Xia Jin-Lin Miao Wen Kang Guoquan Li Di Zhang Mingru Zhang Xiu-Xuan Sun Likun Ding Kui Zhang Junfeng Jia Jin Ding Zhiqin Li Yanyan Jia Lin-Na Liu Zhe Zhang Zhao-Wei Gao Hong Du Na Yao Qing Wang Ke Wang Jie-Jie Geng Bin Wang Ting Guo Ruo Chen Yu-Meng Zhu Li-Juan Wang Qian He Rui-Rui Yao Ying Shi Xiang-Min Yang Jian-Sheng Zhou Yi-Nan Ma Ya-Tao Wang Xue Liang Fei Huo Zhe Wang Yang Zhang Xu Yang Ye Zhang Lu-Hua Gao Ling Wang Xiao-Chun Chen Hao Tang Shuang-Shuang Liu Qing-Yi Wang Zhi-Nan Chen Ping Zhu | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 2 |
| 8 | A generative model of identifying informative proteins from dynamic PPI networks显示文摘Informative proteins are the proteins that play critical functional roles inside cells.They are the fundamental knowledge of translating bioinformatics into clinical practices.Many methods of identifying informative biomarkers have been developed which are heuristic and arbitrary,without considering the dynamics characteristics of biological processes.In this paper,we present a generative model of identifying the informative proteins by systematically analyzing the topological variety of dynamic protein-protein interaction networks(PPINs).In this model,the common representation of multiple PPINs is learned using a deep feature generation model,based on which the original PPINs are rebuilt and the reconstruction errors are analyzed to locate the informative proteins.Experiments were implemented on data of yeast cell cycles and different prostate cancer stages.We analyze the effectiveness of reconstruction by comparing different methods,and the ranking results of informative proteins were also compared with the results from the baseline methods.Our method is able to reveal the critical members in the dynamic progresses which can be further studied to testify the possibilities for biomarker research. | ZHANG Yuan CHENG Yue JIA KeBin ZHANG AiDong | 2014 | Science China(Life Sciences)2014，57，11： | 2 |
| 9 | Co-regulated Protein Functional Modules with Varying Activities in Dynamic PPI Networks显示文摘Current methods for the detection of differential gene expression focus on finding individual genes that may be responsible for certain diseases or external irritants. However, for common genetic diseases, multiple genes and their interactions should be understood and treated together during the exploration of disease causes and possible drug design. The present study focuses on analyzing the dynamic patterns of co-regulated modules during biological progression and determining those having remarkably varying activities, using the yeast cell cycle as a case study. We first constructed dynamic active protein-protein interaction networks by modeling the activity of proteins and assembling the dynamic co-regulation protein network at each time point. The dynamic active modules were detected using a method based on the Bayesian graphical model and then the modules with the most varied dispersion of clustering coefficients, which could be responsible for the dynamic mechanism of the cell cycle, were identified. Comparison of results from our functional module detection with the state-of-art functional module detection methods and validation of the ranking of activities of functional modules using GO annotations demonstrate the efficacy of our method for narrowing the scope of possible essential responding modules that could provide multiple targets for biologists to further experimentally validate. | Yuan Zhang Nan Du Kang L Kebin Jia Aidong Zhang | 2013 | Tsinghua Science and Technology2013，18，5： | 2 |
| 10 | Intercalated 2D nanoclay for emerging drug delivery in cancer therapy显示文摘自然二维(2D ) kaolinite nanoclay 被合并了到一个新兴的药交货系统。kaolinite nanoclay 的基础间距通过不同的链长度的各种各样的器官的客人种类的置闰从 0.72 ~ 4.16 nm 被扩展,它能在药交货增加效率并且减少 doxorubicin (纪录影片) 的毒性。原来的 kaolinite (白陶土) 和白陶土置闰混合物向胰腺的癌症,胃的癌症,前列腺癌症,乳癌, colorectal 癌症,食道的癌症,和区分的甲状腺的房间展出了 biocompatibility 和很低的毒性的高水平癌症。然而,肺癌症和 hepatocellular 癌症房间为药交货搬运人需要更严格的组合、结构、词法的调整。纪录影片白陶土和纪录影片白陶土置闰混合物比处于中立状况在中等酸的答案显示出戏剧性地更快的药版本,并且以一种剂量依赖者方式对十种模型癌症房间文化展出了提高的治疗学的效果。为一个新奇的药交货系统的 2D nanoclay 材料的使用能 feasibly 向高效的 nanotherapeutics 铺平一条道路,与优异 antitumor 功效并且显著地减少副作用。 | Yi Zhang Mei Long Peng Huang Huaming Yang Shi Chang Yuehua Hu Aidong Tang Linfeng Mao | 2017 | Nano Research2017，10，8： | 2 |
| 11 | FindOut: Finding Outliers in Very Large Datasets 显示文摘 | Dantong Yu Gholamhosein Sheikholeslami Aidong Zhang | 2002 | Knowledge and Infor- mation Systems2002，，4： | 1 |
| 12 | Ensuring relaxed atomicity for flexible transactions in multidatabase systems显示文摘 | Zhang Aidong Nodine M Bhargava B | 1994 | ACM SIGMOD Record1994，23，2： | 1 |
| 13 | Analyzing scenery images by monotonic tree显示文摘 | Yunqi Song Aidong Zhang | 2002 | ACM Multimedia Systems Journal2002，8，6： | 1 |
| 14 | ClusterTree: integration of cluster representation and nearest-neighbor search for large data sets with high dimensions显示文摘 | Yu Dantong Zhang Aidong | 2003 | IEEE Transactions on Knowledge and Data Engineering2003，15，5： | 1 |
| 15 | Semantic integration to identify overlappingfunctional modules in protein interaction networks 显示文摘 | Young-Rae Cho Woochang Hwang Murali Ramanathan Aidong Zhang | 2007 | BMC Bioinfonnafics2007，265，8： | 1 |
| 16 | A shrinking-based clustering approach for multidimensional data显示文摘 | Shi Yong Song Yuqing Zhang Aidong | 2005 | IEEE Transactions on Knowledge and Data Engineering2005，，10： | 1 |
| 17 | Extracting semantic concepts from images: a decisive feature pattern mining approach显示文摘 | WANG WEI ZHANG AIDONG | 2006 | Multimedea Systems2006，11，4： | 1 |
| 18 | A multi-resolution content-based retrieval approach for geographic images 显示文摘 | Sheikholeslan Cholamhosein Zhang Aidong | 1999 | GeoInformation1999，3，2： | 1 |
| 19 | NetMedia :streaming multimedia presentations in distributed environments 显示文摘 | Zhang Aidong Song Y Mielke M | 2002 | IEEE Multimedia2002，9，1： | 1 |
| 20 | A nuclear localized protein ZCCHC9 is expressed in cerebral cortex and suppresses the MAPK signal pathway显示文摘The CCHC-type zinc finger motif has numerous biological activities (such as DNA binding and RNA binding) and can also mediate protein-protein interaction. This article gives a primary report about the human ZCCHC9 gene. Protein ZCCHC9 contains four CCHC motifs and is highly conserved in humans, mice, and rats. The whole cDNA sequence of the ZCCHC9 gene has been amplified by PCR and a number of plasmids have been constructed for further study. The results show that ZCCHC9 is localized in the nucleus, and especially concentrated in the nucleolus. It is highly expressed in the brain and testicles of the mouse. This has been confirmed by real-time reverse transcription polymerase chain reaction (RT-PCR). In situ hybridization of the mouse brain indicates that ZCCHC9 is mainly expressed in the cerebral cortex. Reporter gene assay shows that ZCCHC9 suppresses the transcription activities of NF-kappa B and SRE, and may play roles in the Mitogen-Activated Protein Kinase (MAPK) signaling transduction pathway. | Aidong Zhou Jianlin Zhou Liping Yang Mingjun Liu Hong Li Su Xu Mei Han Jian Zhang | 2008 | Journal of Genetics and Genomics2008，35，8： | 1 |
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