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| 1 | Population Genomic Analysis and De Novo Assembly Reveal the Origin of Weedy Rice as an Evolutionary Game显示文摘Crop weediness,especially that of weedy rice(Oryza sativa f.spontanea),remains mysterious.Weedy rice possesses robust ecological adaptability;however,how this strain originated and gradually formed proprietary genetic features remains unclear?Here,we demonstrate that weedy rice at Asian high latitudes(WRAH)is phylogenetically well defined and possesses unselected genomic characteristics in many divergence regions between weedy and cultivated rice.We also identified novel quantitative trait loci underlying weedy-specific traits,and revealed that a genome block on the end of chromosome 1 is associated with rice weediness.To identify the genomic modifications underlying weedy rice evolution,we generated the first de novo assembly of a high-quality weedy rice genome(WR04-6),and conducted a comparative genomics study between WR04-6 with other rice reference genomes.Multiple lines of evidence,including the results of demographic scenario comparisons,suggest that differentiation between weedy rice and cultivated rice was initiated by genetic improvement of cultivated rice and that the essence of weediness arose through semi-domestication.A plant height model further implied that the origin of WRAH can be modeled as an evolutionary game and indicated that strategy-based selection driven by fitness shaped its genomic diversity. | Jian Sun Dianrong Ma Liang Tang Minghui Zhao Guangchen Zhangh Wenjia Wang Jiayu Song Xiang Li Zimeng Liu Wenxing Zhang Quan Xu Yuncheng Zhou Jianzhong Wu Toshio Yamamoto Fei Dai Yan Lei Song Li Gang Zhou Hongkun Zheng Zhengjin Xu Wenfu Chen | 2019 | Molecular Plant2019，12，5： | 9 |
| 2 | Effects of different doses in continuous veno-venous hemofiltration on plasma lactate in critically ill patients显示文摘 | Liu Yongjun Ouyang Bin Chen Juan Chen Minying Ma Jie Wu Jianfeng Huang Shunwei Li Lifen Liu Zimeng Guan Xiangdong | 2014 | Chinese Medical Journal2014，，10： | 4 |
| 3 | Development of a method to quantify total and free irinotecan and 7-ethyl-10-hydroxycamptothecin(SN-38) for pharmacokinetic and bio-distribution studies after administration of irinotecan liposomal formulation显示文摘In 2015,liposomal formulation of irinotecan(ONIVYDE)has been approved by FDA and widely applied in the treatment of pancreatic cancer.ONIVYDE is a novel liposome formulation,entrapping CPT-11 in the aqueous core of vesicles using a modified gradient loading method.Due to toxicity concerns,it is essential to explore a rapid and reliable method to effectively isolate and quantify the non-liposomal,namely,free CPT-11and total CPT-11 in plasma.This study focuses on separation of non-liposomal CPT-11,evaluation of the pharmacokinetics of free CPT-11 and total CPT-11 and bio-distribution after intravenous administration of CPT-11 liposome.Free CPT-11 in plasma was separated by solid-phase extraction(SPE).The amount of total CPT-11 and main metabolite 7-ethyl-10-hydroxycamptothecin(SN-38)in plasma was quantified by ultra-performance liquid chromatography–MS/MS.The calibration curves fitted well and lower limit of quantitation for SN-38,free CPT-11,total CPT-11 and CPT-11 in tissue and were 5 ng/ml,10 ng/ml,4.44 ng/ml and 25 ng/ml respectively.The recoveries,precision and accuracy of the method appear satisfactory.Using this method,the pharmacokinetics and bio-distribution of CPT-11 liposome formulation after an intravenous dose of 2.5 mg/kg were then investigated. | Wenqian Yang Zimeng Yang Jieru Liu Dan Liu Yongjun Wang | 2019 | Asian Journal of Pharmaceutical Sciences2019，14，6： | 2 |
| 4 | iTRAQ-Based Proteomics Investigation of Critical Response Proteins in Embryo and Coleoptile During Rice Anaerobic Germination显示文摘Direct-seeding of rice has become popular in recent years due to its low cost and convenience,however,hypoxic condition limits seedling establishment.In this study,weedy rice WR04-6 with high germination ability under anaerobic conditions was used as a gene donor,and we successfully improved the seedling establishment rate of rice cultivar Qishanzhan(QSZ)based on selection of a new rice line R42 from the recombinant inbred line population.R42 inherited high anaerobic germination(AG)ability,and was used for isobaric tags for relative and absolute quantitation(iTRAQ)-based comparative proteomic studies with QSZ to further explore the molecular mechanism of AG.A total of 719 differentially abundant proteins(DAPs)were shared by R42 and QSZ responded to AG,and thus defined as common response DAPs.A total of 300 DAPs that responded to AG were only identified from R42,which were defined as tolerance-specific DAPs.The common response and tolerance-specific DAPs had similar biochemical reaction processes and metabolic pathways in response to anoxic stress,however,they involved different proteins.The tolerance-specific DAPs were involved in amino acid metabolism,starch and sucrose metabolism,tricarboxylic acid cycle pathway,ethylene synthesis pathway,cell wall-associated proteins and activity of active oxygen scavenging enzyme.The in silico protein-protein interactions for the top 60 DAPs indicated that tolerance-specific DAPs had relatively independent protein interaction networks in response to an anoxic environment compared with common response DAPs.The results of physiological indicators showed thatα-amylase and superoxide dismutase activities of R42 were significantly increased under anoxic conditions compared with aerobic conditions.Multiple lines of evidence from western blot,physiological analysis and quantitatDirect seeding of rice has become popular in recent years due to its low cost and convenience,however,hypoxic conditions can limit seedling establishment.In the present study,weedy rice WR04-6 with high germination ability in anaerobic conditions was used as the gene donor and successfully improved the seedling establishment rate of rice cultivar Qishanzhan(QSZ)based on selection of a new rice line R42 from the recombinant inbred line(RIL)population.R42 inherited the had high anaerobic germination(AG)ability,which was used for the isobaric tags for relative and absolute quantitation(iTRAQ)based comparative proteomic studies with QSZ to further explore the molecular mechanism of AG.A total of 719 differentially abundant proteins(DAPs)shared by R42 and QSZ responded to AG and were thus defined as common response DAPs.A total of 300 DAPs that responded to AG were only identified from R42,which were defined as tolerance-specific DAPs.The common response and tolerance-specific DAPs had similar biochemical reaction processes and metabolic pathways in response to anoxic stress,however they involved different proteins.The 300 tolerance-specific DAPs were involved in amino acid metabolism,starch and sucrose metabolism,TCA cycle pathways,ethylene synthesis pathway,cell wall-associated proteins and activity of active oxygen scavenging enzyme.The in silico protein-protein interactions for the top 60 DAPs indicated that tolerance-specific DAPs had relatively independent protein interaction networks in response to an anoxic environment compared with common response DAPs.The results of physiological indicators showed thatα-amylase and SOD activities of R42 were significantly increased under anoxic conditions compared with aerobic conditions.Multiple lines of evidence from western blot,physiological analyses and real-time PCR jointly supported the reliability of proteomics data.In summary,our findings deepened the understanding of the molecular mechanism for the rice response to AG.ive real-time PCR jointly supported the reliability of proteomics data.In summary,our findings deepened the understanding of the molecular mechanism for the rice response to AG. | Zhang Guangchen Liu Zimeng Liu Yoxihong Kuya Noriyuki HuAYuchen Shi Hongru Zhao Weilin Han Yuqing Yamamoto Toshio Chen Wenfu Sun Jian | 2021 | Rice science2021，28，4： | 2 |
| 5 | Lanthanum-dopedα-Ni(OH)_(2)1D-2D-3D hierarchical nanostructures for robust bifunctional electro-oxidation显示文摘The development of advanced electrocatalysts for electro-oxidation reactions has attracted much attention because of the critical role of such electrocatalysts in improving the overall efficiency of coupled hydrogen production.We have developed an efficient lanthanum-dopedα-Ni(OH)_(2) bifunctional catalyst with a 1D-2D-3D hierarchical nanostructure.It shows superior activity and stability in the anodic oxygen evolution reaction(OER)and urea oxidation reaction(UOR).Enrichment of the edge sites and conducting La doping inα-Ni(OH)_(2) phase enable formation and stabilization of abundant local Ni^(3+)ions.This guarantees ultralow onset potentials in electro-oxidation reactions.The 1D-2D-3D hierarchical nanostructure significantly boosts the in situ generation of high-valence active species,which results in efficient and stable OER and UOR performances,and the synergistic merit of doping-induced facile reaction kinetics.Because of the structural benefits of a large surface area,charge-transfer promotion,good structural stability,and bifunctionality,a 1%La-dopedα-Ni(OH)_(2) hierarchical nanostructure gives superior OER and UOR performances with low overpotentials,large catalytic current densities,and excellent operational stability.It is therefore a promising catalyst for use in simultaneous alkaline wastewater treatment and hydrogen production. | Zimeng Wei Wenbin Sun Shanshan Liu Jindi Qi Luyao Kang jiechen Li Shanshan Lou Junfeng Xie Bo Tanga Yi Xie | 2021 | Particuology2021，19，4： | 1 |
| 6 | CFD modeling of a UV - LED photocatalytic odor abatement process in a continuous reactor 显示文摘 | Zimeng Wang Jing Liu Yuancan Dai | 2012 | Journal of Hazardous Materials2012，，： | 1 |
| 7 | CFDmodeling of a UV-LED photocatalytic odor abatement process in a continuous reactor显示文摘 | Wang Zimeng Liu Jing Dai Yuancan | 2012 | Journal of Hazardous Materials2012，，： | 1 |
| 8 | Dexmedetomidine administration before ,but not after ,ischemia attenuates intestinal injury induced by intestinal ischemia-reperfusion in rats 显示文摘 | Zhang Xuyu Liu Zimeng Wen Shihong | 2012 | Anesthesiology2012，116，5： | 1 |
| 9 | An overview of bioelectrokinetic and bioelectrochemical remediation of petroleum-contaminated soils显示文摘The global problem of petroleum contamination in soils seriously threatens environmental safety and human health.Current studies have successfully demonstrated the feasibility of bioelectrokinetic and bioelectrochemical remediation of petroleum-contaminated soils due to their easy implementation,environmental benignity,and enhanced removal efficiency compared to bioremediation.This paper reviewed recent progress and development associated with bioelectrokinetic and bioelectrochemical remediation of petroleum-contaminated soils.The working principles,removal efficiencies,affecting factors,and constraints of the two technologies were thoroughly summarized and discussed.The potentials,challenges,and future perspectives were also deliberated to shed light on how to overcome the barriers and realize widespread implementation on large scales of these two technologies. | Jun Lan Fang Wen Yongxiang Ren Guangli Liu Yi Jiang Zimeng Wang Xiuping Zhu | 2023 | Environmental Science and Ecotechnology2023，，4： | 0 |
| 10 | Research on Practical Teaching of Ideological and Political Courses in Colleges and Universities and Collaborative Education in the Second Classroom显示文摘In today’s society,the need for talent training is of utmost importance due to rapid development.To achieve high-quality talent training,we need to focus on building a collaborative education mechanism.Practical teaching and the second classroom,which serve as important carriers of educational talents,must align their educational goals and complement each other.This article explores the collaborative education mechanism of the second classroom and practical teaching for the ideological and political courses in colleges and universities.It proposes reasonable construction measures to provide some guidelines for teaching work. | Luhua Xiao Pingping Liu Zimeng Li | 2024 | Journal of Contemporary Educational Research2024，8，1： | 0 |
| 11 | Rheological behavior of mixed system of ionic liquid [C8mim]Br and sodium oleate in water显示文摘我们在离子的液体表面活化剂构造的 wormlike 微粒的 rheological 行为上报导[C 8 mim ] Br (1-octyl-3-methylimidazolium 溴化物) 和在水的答案的 anionic 表面活化剂钠油酸盐(NaOA ) 。表面活化剂作文,全部的表面活化剂集中,增加的盐,和温度的效果被调查。在更低的集中的占优势的表面活化剂效果和在更高的集中的领先的 cosolvent 效果[C 8 mim ] Br 可以是为在有表面活化剂作文和全部的表面活化剂集中的变化的关键 rheological 参数的生长得很好的最大值的外观的主要原因。海甘蓝海甘蓝阴谋证明系统(在 0.170.35 摩尔以内的全部的表面活化剂集中下降吗? | Zimeng HE Ling YUE Meng LI Yazhuo SHANG Honglai LIU | 2015 | Frontiers of Chemical Science and Engineering2015，9，2： | 0 |
| 12 | Elaborately engineering of lipid nanoparticle for targeting delivery of siRNA and suppressing acute liver injury显示文摘Small interfering RNA(siRNA)-based gene silencing has been considered as a potential therapy modality against inflammatory diseases.Nevertheless,the effective delivery of siRNA to desired destination still remains challenging due to poor stability,high molecular weight and negative charge.Currently,ionizable lipid nanoparticle(LNP)has been extensively used as vector for effective delivery of siRNA.Herein,we report a mannose-modified LNP(M-MC_(3) LNP@TNFα)loading tumor necrosis factorα(TNFα)siRNA for targeting liver macrophages,achieving effectively inhibit acute liver injury.The M-MC_(3) LNP@TNFαnot only increases the internalization of LNP by macrophages,but also enhances the gene silencing efficiency of TNFαin vitro.Additionally,the M-MC_(3) LNP@TNFαexhibits higher accumulation in liver of healthy mice than that of MC_(3) LNP@TNFα(un-modified LNP)owing to the targeting effect of mannose.As expected,the M-MC_(3) LNP@TNFαsignificantly suppresses the expression of TNFαand ameliorates liver damage in acute liver injury model.Such a LNP targeting siRNA delivery holds great potential for the treatment of diseases associated with liver in the future. | Qiu Wang Qikun Jiang Dan Li Zimeng Yang Lin Gao Fan Liu Chang Li Yao Feng Zhonggui He Cong Luo Jin Sun | 2024 | Chinese Chemical Letters2024，35，2： | 0 |
| 13 | Automatic Sleep Staging Algorithm Based on Random Forest and Hidden Markov Model显示文摘In the field of medical informatics,sleep staging is a challenging and timeconsuming task undertaken by sleep experts.According to the new standard of the American Academy of Sleep Medicine(AASM),the stages of sleep are divided into wakefulness(W),rapid eye movement(REM)and non-rapid eye movement(NREM)which includes three sleep stages(N1,N2 and N3)that describe the depth of sleep.This study aims to establish an automatic sleep staging algorithm based on the improved weighted random forest(WRF)and Hidden Markov Model(HMM)using only the features extracted from double-channel EEG signals.The WRF classification model focuses on reducing the bias of the imbalance data,while the HMM model focuses on improving the detection rate of sleep staging through the relationship between adjacent sleep stages.In particular,the improved weighted RF classification model can increase the recognition rate of the N1 stage.In addition,the method of removing features with low variance is used to select meaningful and contributing feature parameters for model training.This is an innovative content of this paper.The sleep EEG data are first segmented into 30 s epochs,and the feature parameters of the epoch data are extracted from the double-channel by applying continuous wavelet packet transform(WPT).Each epoch is then segmented into 29 subepochs of 2 s long with 1 s overlap,and the frequency domain features and statistical features of each subepoch are extracted.The performance of the proposed method is tested by evaluating the accuracy(AC),Kappa coefficient,Recall(R),Precision(P)and F1-score(F1).In the Sleep-EDF database,the overall AC and Kappa coefficient obtained by WRF are 93.20%and 0.890,respectively using the subject-non-independent test.In the 10 sc*and 10 st*Sleep-EDF Expanded database,the overall AC and Kappa coefficient obtained by proposed method are 91.97%and 0.874,respectively using the subject-independent test.The best AC and Kappa coefficient of single subject can reach 96.3%and 0.912,respectively.Experimental results show that the performance of the proposed method is competitive with the most current methods and results,and the recognition rate of N1 stage is significantly improved. | Junbiao Liu Duanpo Wu Zimeng Wang Xinyu Jin Fang Dong Lurong Jiang Chenyi Cai | 2020 | Computer Modeling in Engineering & Sciences2020，，4： | 0 |
| 14 | Water:The lifeblood of the Earth-human system revealed in elemental correlations显示文摘1.Elements and human history The rich chemistry of Earth's elements played an inherent and multifaceted role in shaping the course of human history.Generally,the more inert an element is,the simpler it becomes to extract it from minerals,facilitating its use in the crafting of tools,devices,and weapons. | Zimeng WANG Yongsheng LIU Anhuai LU Yanxin WANG | 2023 | Science China Earth Sciences2023，66，10： | 0 |
| 15 | 3D hypothesis clustering for cross-view matching in multiperson motion capture显示文摘We present a multiview method for markerless motion capture of multiple people. The main challenge in this problem is to determine crossview correspondences for the 2 D joints in the presence of noise. We propose a 3 D hypothesis clustering technique to solve this problem. The core idea is to transform joint matching in 2 D space into a clustering problem in a 3 D hypothesis space. In this way, evidence from photometric appearance, multiview geometry, and bone length can be integrated to solve the clustering problem efficiently and robustly. Each cluster encodes a set of matched 2 D joints for the same person across different views, from which the 3 D joints can be effectively inferred. We then assemble the inferred 3 D joints to form full-body skeletons for all persons in a bottom–up way. Our experiments demonstrate the robustness of our approach even in challenging cases with heavy occlusion,closely interacting people, and few cameras. We have evaluated our method on many datasets, and our results show that it has significantly lower estimation errors than many state-of-the-art methods. | Miaopeng Li Zimeng Zhou Xinguo Liu | 2020 | Computational Visual Media2020，6，2： | 0 |
| 16 | Time-restricted feeding downregulates cholesterol biosynthesis program via RORγ-mediated chromatin modification in porcine liver organoids显示文摘Background: Time-restricted feeding(TRF) is a dieting strategy based on nutrients availability and diurnal rhythm,shown to improve lipid metabolism efficiency. We have demonstrated previously that retinoic acid-related(RAR)orphan receptor(ROR) γ is the primary transcription factor controlling cholesterol(CHO) biosynthesis program of animals. However, the functional role of RORγ in liver physiology of pigs in response to TRF has not been determined, largely due to the lack of functional models and molecular tools. In the present study, we established porcine liver organoids and subjected them to restricted nutrients supply for 10-h during the light portion of the day.Results: Our results showed that TRF regimen did not alter hepatocyte physiology, including unchanged cell viability, caspase 3/7 enzyme activity and the gene signature of cell proliferation in porcine liver organoids,compared to the control group(P > 0.05). Furthermore, we found that TRF downregulated the hepatic CHO biosynthesis program at both mRNA and protein levels, along with the reduced cellular CHO content in porcine liver organoids(P < 0.05). Using unbiased bioinformatic analysis of a previous ChIP-seq data and ChIP-qPCR validation, we revealed RORγ as the predominant transcription factor that responded to TRF, amongst the 12 targeted nuclear receptors(NRs)(P < 0.05). This was likely through RORγ direct binding to the MVK gene(encoding mevalonate kinase). Finally, we showed that RORγ agonists and overexpression enhanced the enrichment of cofactor p300, histone marks H3 K27 ac and H3K4me1/2, as well as RNA polymerase II(Pol-II) at the locus of MVK, in TRF-porcine liver organoids, compared to TRF-vector control(P < 0.05).Conclusions: Our findings demonstrate that TRF triggers the RORγ-mediated chromatin remodeling at the locus of CHO biosynthesis genes in porcine liver organoids and further improves lipid metabolism. | Kexin Zhang Hao Li Zimeng Xin Yanwei Li Xiaolong Wang Yun Hu Haoyu Liu Demin Cai | 2021 | Journal of Animal Science and Biotechnology2021，12，1： | 0 |
| 17 | Antidepressant-like Effects of Flavonoids from Pteridium aquilinum var.latiusculum显示文摘[Objectives]To explore the antidepressant-like effects of PAFL and its potential mechanisms.[Methods]This study used PAFL in mice under various conditions and analyzed the results.The antidepressant-like effects of flavonoids from Pteridium aquilinum(PAFL)and its possible action mechanism were discussed.[Results]PAFL(5 mg/kg,i.p.)exhibited markedly antidepressant-like activities,which could be reversed by treatment with p-chlorophenylalanine(an inhibitor of 5-HT synthesis),haloperidol(a non-selective D 2 receptor antagonist)and N-methyl-D-aspartic acid(an agonist at the glutamate site).Meanwhile,PAFL also effectively increased the hippocampus 5-HT,DA,and Glu levels of mice exposed to TST and FST.The LPS-induced antidepressant behavioral experiment showed that PAFL(1 and 5 mg/kg,i.p.)and fluoxetine(20 mg/kg,i.p.)effectively reduced the immobility time,and increase activity time in OFT.PAFL pretreatment significantly reduced IL-6 and TNF-αserum levels and improve the changes of hippocampal SOD and MDA oxidative stress indicators.Furthermore,PAFL preconditioning could up-regulate BDNF and TrκB contents in hippocampus and down-regulate IκB-αand NF-κB phosphorylation.[Conclusions]The antidepressant-like effects of PAFL might be mediated by the 5-HT,DAergic and Gluergic systems,and the mechanisms of anti-depressant effects of PAFL might be via the alterations of animal behaviors,hippocampus inflammation and neurotrophy,which might be attributed by the BDNF/TrκB/NF-κB signaling pathway. | Zimeng FENG Xiang HAN Shuangli LIU Xiaohui WANG | 2023 | Medicinal Plant2023，14，1： | 0 |
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