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| 1 | De novo mutation in ATP6V1B2 impairs lysosome acidification and causes dominant deafness-onychodystrophy syndrome显示文摘 | Yongyi Yuan Jianguo Zhang Qing Chang Jin Zeng Feng Xin Jianjun Wang Qingyan Zhu Jing Wu Jingqiao Lu Weiwei Guo Xukun Yan Hui Jiang Binfei Zhou Qi Li Xue Gao Huijun Yuan Shiming Yang Dongyi Han Zixu Mao Ping Chen Xi Lin Pu Dai | 2014 | Cell Research2014，24，11： | 13 |
| 2 | Rambutan-like hollow carbon spheres decorated with vacancy-rich nickel oxide for energy conversion and storage显示文摘Transition metal oxides hold great promise for lithium-ion batteries(LIBs)and electrocatalytic water splitting because of their high abundance and high energy density.However,designing and fabrication of efficient,stable,high power density electrode materials are challenging.Herein,we report rambutan-like hollow carbon spheres formed by carbon nanosheet decorated with nickel oxide(NiO)rich inmetal vacancies(denoted as h-NiO/C)as a bifunctional electrode material for LIBs and electrocatalytic oxygen evolution reaction(OER).When being used as the anode of LIBs,the h-NiO/C electrode shows a large initial capacity of 885mAh g−1,a robust stability with a high capacity of 817mAh g−1 after 400 cycles,and great rate capability with a high reversible capacity of 523mAh g−1 at 10Ag−1 after 600 cycles.Moreover,working as an OER electrocatalyst,the h-NiO/C electrode shows a small overpotential of 260mV at 10mAcm−2,a Tafel slope of 37.6mVdec−1 along with good stability.Our work offers a cost-effective method for the fabrication of efficient electrode for LIBs and OER. | Zixu Sun Xinghui Wang Hu Zhao See W.Koh Junyu Ge Yunxing Zhao Pingqi Gao Guangjin Wang Hong Li | 2020 | Carbon Energy2020，2，1： | 4 |
| 3 | Genome editing in plants with MAD7 nuclease显示文摘MAD7 is an engineered nuclease of the Class 2 type V-A CRISPR-Cas(Cas12 a/Cpf1)family with a low level of homology to canonical Cas12 a nucleases.It has been publicly released as a royalty-free nuclease for both academic and commercial use.Here,we demonstrate that the CRISPR-MAD7 system can be used for genome editing and recognizes T-rich PAM sequences(YTTN)in plants.Its editing efficiency in rice and wheat is comparable to that of the widely used CRISPR-Lb Cas12 a system.We develop two variants,MAD7-RR and MAD7-RVR that increase the target range of MAD7,as well as an M-AFID(a MAD7-APOBEC fusion-induced deletion)system that creates predictable deletions from 50-deaminated Cs to the MAD7-cleavage site.Moreover,we show that MAD7 can be used for multiplex gene editing and that it is effective in generating indels when combined with other CRISPR RNA orthologs.Using the CRISPR-MAD7 system,we have obtained regenerated mutant rice and wheat plants with up to 65.6%efficiency. | Qiupeng Lin Zixu Zhu Guanwen Liu Chao Sun Dexing Lin Chenxiao Xue Shengnan Li Dandan Zhang Caixia Gao Yanpeng Wang Jin-Long Qiu | 2021 | Journal of Genetics and Genomics2021，48，6： | 4 |
| 4 | Taurine-upregulated gene 1 contributes to cancers through sponging microRNA显示文摘Long non-coding RNAs(lncRNAs)are a class of RNAs whose transcripts are more than 200 nucleotides in length and lack protein-coding ability.Taurine-upregulated gene 1(TUG1),a novel cancer-related lncRNA,has been documented to be abnormally expressed in various types of cancers and act as an oncogene or anti-oncogene.It has been considered previously that TUG1 is closely related to the cell proliferation,invasion,metastasis,and apoptosis of cancer.In recent years,it has been found that TUG1 acts as a microRNA(miRNA)sponge to indirectly regulate the expression of the miRNA target gene and dominates cancer progression in several types of cancers.However,TUG1 also binds to different miRNAs to produce diverse regulatory mechanisms in the same cancer.TUG1 is expected to be a biomarker and a new therapeutic target for the diagnosis and prognosis of certain cancers.In this review,we highlight the up-to-date original studies that focus on the role of TUG1 sponging miRNA in cancers and summarize the function of TUG1 in cancer progression.The novel TUG1–miRNA regulatory network is comprehensively and minutely included in this review.We hope that this review will help readers obtain a more detailed knowledge of the molecular mechanism by which TUG1 sponging miRNA plays its role in cancers,and provide some insights and directions for future cancer research. | Hui Zhou Zixu Gao Fusheng Wan | 2019 | Acta Biochimica et Biophysica Sinica2019，51，2： | 2 |
| 5 | Integrated microdevice with a windmill-like hole array for the clog-free,efficient,and self-mixing enrichment of circulating tumor cells显示文摘Circulating tumor cells(CTCs)have tremendous potential to indicate disease progression and monitor therapeutic response using minimally invasive approaches.Considering the limitations of affinity strategies based on their cost,effectiveness,and simplicity,size-based enrichment methods that involve low-cost,label-free,and relatively simple protocols have been further promoted.Nevertheless,the key challenges of these methods are clogging issues and cell aggregation,which reduce the recovery rates and purity.Inspired by the natural phenomenon that the airflow around a windmill is disturbed,in this study,a windmill-like hole array on the SU-8 membrane was designed to perturb the fluid such that cells in a fluid would be able to self-mix and that the pressure acting on cells or the membrane would be dispersed to allow a greater velocity.In addition,based on the advantages of fluid coatings,a lipid coating was used to modify the membrane surface to prevent cell aggregation and clogging of the holes.Under the optimal conditions,recovery rates of 93%and 90%were found for A549 and HeLa cells in a clinical simulation test of our platform with a CTC concentration of 20-100 cells per milliliter of blood.The white blood cell(WBC)depletion rate was 98.7%(n=15),and the CTC detection limit was less than 10 cells per milliliter of blood(n=6).Moreover,compared with conventional membrane filtration,the advantages of the proposed device for the rapid(2 mL/min)and efficient enrichment of CTCs without clogging were shown both experimentally and theoretically.Due to its advantages in the efficient,rapid,uniform,and clog-free enrichment of CTCs,our platform offers great potential for metastatic detection and therapy analyses. | Hao Li Jinze Li Zhiqi Zhang Zhen Guo Changsong Zhang Zixu Wang Qiuquan Guo Chao Li Chuanyu Li Jia Yao Anran Zheng Jingyi Xu Qingxue Gao Wei Zhang Lianqun Zhou | 2022 | Microsystems & Nanoengineering2022，8，1： | 1 |
| 6 | External rectal prolapse:abdominal or perineal repair for men?A retrospective cohort study显示文摘Background:External rectal prolapse is a relatively rare disease,in which male patients account for a minority.The selection of abdominal repair or perineal repair for male patients has rarely been investigated.Methods:Fifty-one male patients receiving abdominal repair(laparoscopic ventral rectopexy)or perineal repair(Delorme or Altemeier procedures)at the Sixth Affiliated Hospital of Sun Yat-sen University(Guangzhou,China)between March 2013 and September 2019 were retrospectively analysed.We compared the recurrence,complication rate,post-operative defecation disorder,length of stay,and quality of life between the abdominal and perineal groups.Results:Of the 51 patients,45 had a complete follow-up,with a median of 48.5 months(range,22.8–101.8 months).A total of 35 patients were under age 40 years.The complication rate associated with abdominal repair was less than that associated with perineal repair(0%vs 20.7%,P=0.031)and the recurrence rate was also lower(9.5%vs 41.7%,P=0.018).Multivariate analysis showed that perineal repair(odds ratio,9.827;95%confidence interval,1.296–74.50;P=0.027)might be a risk factor for recurrence.Moreover,only perineal repair significantly improved post-operative constipation status(preoperative vs post-operative,72.4%vs 25.0%,P=0.001).There was no reported mortality in either of the groups.No patient’s sexual function was affected by the surgery.Conclusions:Both surgical approaches were safe in men.Compared with perineal repair,the complication rate and recurrence rate for abdominal repair were lower.However,perineal repair was better able to correct constipation. | Bang Hu Qi Zou Zhenyu Xian Dan Su Chao Liu Li Lu Minyi Luo Zixu Chen Keyu Cai Han Gao Hui Peng Wuteng Cao Donglin Ren | 2022 | Gastroenterology Report2022，10，1： | 0 |
| 7 | Assessment of complete lymph node dissection in patients with melanoma:A systemic review and meta-analysis显示文摘Background: Complete lymph node dissection(CLND) for patients with melanoma remains controversial. This meta-analysis aimed to compare the prognoses and complications between the CLND and control groups(patients who receive adjuvant treatment or observation only) in patients with sentinel lymph node(SLN)-positive melanoma.Methods: The Pub Med, Embase, Cochrane, and Web of Science databases were searched for cohort studies and randomized clinical trials(RCTs) conducted between 1964 and 2022, and the quality of the studies was assessed using the Cochrane risk-of-bias tool and Newcastle-Ottawa Scale. Hazard ratios(HR) or risk ratios(RR) with 95%confidence intervals(CIs) were calculated for each outcome. Heterogeneity and sensitivity tests were also conducted, and publication bias tests were performed when the pooled number of studies was >10.Results: Fifteen studies, including 11 cohort studies and 4 RCTs, were enrolled and assessed for quality. Analysis of overall survival showed no significant difference between the CLND and control groups(HR=1.02, 95% CI:0.69–1.51, P=0.922). Similarly, recurrence-free survival(HR=0.84, 95% CI: 0.6–1.16, P=0.287), disease-free survival(HR=1.06, 95% CI: 0.65–1.72, P=0.82), and disease-specific survival(HR=0.84, 95% CI: 0.59–1.21,P=0.355) showed no difference between the two groups. CLND did not reduce the risk of recurrence(RR=0.98,95% CI: 0.8–1.2, P=0.851).Conclusion: Remarkably, patients who underwent CLND were more likely to have complications such as flap necrosis and lymphedema than the controls. CLND does not improve patient prognosis and may increase the incidence of complications. | Jiangying Xuan Ming Zhu Lu Wang Zixu Gao Kangjie Shen Ming Ren Yanlin Li Chuanyuan Wei Jianying Gu | 2023 | Chinese Journal Of Plastic and Reconstructive Surgery2023，5，4： | 0 |
| 8 | Re-Exploring Biomarkers and Therapeutic Targets in Primary Melanoma Patients: Insights from Network-Based Analysis of Microarray Data显示文摘Objective To identify novel biomarkers and therapeutic targets for primary melanoma using network-based microarray data analysis.Methods Eligible microarray datasets from the Gene Expression Omnibus(GEO)database were used to identify differentially expressed genes(DEGs).The protein-protein interaction(PPI)network,Gene Ontology(GO),and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were performed to identify hub genes and pathways that might affect the survival of melanoma patients.Immunohistochemistry results obtained from the Human Protein Atlas(HPA)database confirmed the protein expression levels of hub genes.The Cancer Genome Atlas(TCGA)database was used to further verify the gene expression levels and conduct survival analysis.Results Three microarray datasets(GSE3189,GSE15605,and GSE46517)containing 122 melanoma and 30 normal skin tissue samples were included.A total of 262 common differentially expressed genes(cDEGs)were identified based on three statistical approaches(Fisher’s method,the random effects model(REM),and vote counting)with strict criteria.Of these,two upregulated genes,centromere protein F(CENPF)and pituitary tumortransforming gene 1(PTTG1),were selected as hub genes.HPA and TCGA database analyses confirmed that CENPF and PTTG1 were overexpressed in melanoma.Survival analysis showed that high expression levels of CENPF were significantly correlated with decreased overall survival(OS)(P=0.028).Conclusion The expression level of CENPF was significantly upregulated in melanoma and correlated with decreased OS.Thus,CENPF may represent a novel biomarker and therapeutic target for melanoma patients. | Kangjie SHEN Chuanyuan WEI Yi XIE Lu WANG Shuyu WANG Ming REN Xinyi DENG Daohe WANG Zixu GAO Zihao FENG Jianying GU | 2020 | Chinese Journal Of Plastic and Reconstructive Surgery2020，2，4： | 0 |
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