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| 1 | Chimeric antigen receptor-modified T cells for the immunotherapy of patients with EGFR-expressing advanced relapsed/refractory non-small cell lung cancer显示文摘The successes achieved by chimeric antigen receptor-modified T(CAR-T) cells in hematological malignancies raised the possibility of their use in non-small lung cancer(NSCLC). In this phase I clinical study(NCT01869166), patients with epidermal growth factor receptor(EGFR)-positive(>50% expression), relapsed/refractory NSCLC received escalating doses of EGFR-targeted CAR-T cell infusions. The EGFR-targeted CAR-T cells were generated from peripheral blood after a 10 to 13-day in vitro expansion. Serum cytokines in peripheral blood and copy numbers of CAR-EGFR transgene in peripheral blood and in tissue biopsy were monitored periodically. Clinical responses were evaluated with RECIST1.1 and immune-related response criteria, and adverse events were graded with CTCAE 4.0. The EGFR-targeted CAR-T cell infusions were well-tolerated without severe toxicity. Of 11 evaluable patients, two patients obtained partial response and five had stable disease for two to eight months. The median dose of transfused CAR+ T cells was 0.97×10~7 cells kg^(-1)(interquartile range(IQR), 0.45 to 1.09×10~7 cells kg^(-1)). Pathological eradication of EGFR positive tumor cells after EGFR-targeted CAR-T cell treatment can be observed in tumor biopsies, along with the CAR-EGFR gene detected in tumor-infiltrating T cells in all four biopsied patients. The EGFR-targeted CAR-T cell therapy is safe and feasible for EGFR-positive advanced relapsed/refractory NSCLC. | Kaichao Feng Yelei Guo Hanren Dai Yao Wang Xiang Li Hejin Jia Weidong Han | 2016 | Science China(Life Sciences)2016，59，5： | 62 |
| 2 | Comparison of endotracheal intubation with the Shikani Optical Stylet using the left molar approach and direct laryngoscopy显示文摘 | YAO Yun-tai JIA Nai-guang LI Cheng-hui ZHANG Ya-jun YIN Yi-qing | 2008 | Chinese Medical Journal2008，，14： | 41 |
| 3 | Natural variation in Ghd7.1 plays an important role in grain yield and adaptation in rice显示文摘 | Wenhao Yan Haiyang Liu Xiangchun Zhou Qiuping Li Jia Zhang Li Lu Touming Liu Haijun Liu Chengjun Zhang Zhanyi Zhang Guojing Shen Wen Yao Huaxia Chen Sibin Yu Weibo Xie Yongzhong Xing | 2013 | Cell Research2013，23，7： | 39 |
| 4 | Control of Grain Size and Weight by the OsMKKK10-OsM KK4-OsMAPK6 Signaling Pathway in Rice显示文摘谷物尺寸是在庄稼决定谷物产量的关键农学的特点之一。然而,在庄稼位于谷物尺寸控制下面的机制留下逃犯。这里,我们证明断然表明小径的 OsMKKK10-OsMKK4-OsMAPK6 在米饭调整谷物尺寸和重量。在米饭,当组成地活跃的 OsMKKK10 (CA-OsMKKK10 ) 的 overexpression 导致大、重的谷物,长圆锥花序,和高植物时, OsMKKK10 功能的损失导致小、轻的谷物,短圆锥花序,和半矮子植物。OsMKKK10 交往与并且 phosphorylates OsMKK4。我们识别了 OsMKK4 gain-of-function 异种(large11-1D )? 那生产大、重的谷物。OsMKK4 large11-1D 等位基因编码的 A227T 比 OsMKK4 举办更强壮的 kinase 活动。植物 overexpressing OsMKK4 (OsMKK4-DD ) 的一种组成地活跃的形式也生产大谷物。进一步生物化学、基因的分析表明 OsMKKK10, OsMKK4,和 OsMAPK6 在一条普通小径工作控制谷物尺寸。一起拿,我们的学习在米饭为谷物尺寸和重量的 OsMKKK10-OsMKK4-OsMAPK6 调停串联的控制建立一个重要基因、分子的框架。 | Ran Xu Penggen Duan Haiyue Yu Zhengkui Zhou Baolan Zhang Ruci Wang Jing Li Guozheng Zhang Shangshang Zhuang Jia Lyu Na Li Tuanyao Chai Zhixi Tian Shanguo Yao Yunhai Li | 2018 | Molecular Plant2018，11，6： | 33 |
| 5 | Anti-SARS-CoV-2 activities in vitro of Shuanghuanglian preparations and bioactive ingredients显示文摘Human infection with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)causes coronavirus disease 2019(COVID-19)and there is no cure currently.The 3CL protease(3CLpro)is a highly conserved protease which is indispensable for CoVs replication,and is a promising target for development of broad-spectrum antiviral drugs.In this study we investigated the anti-SARS-CoV-2 potential of Shuanghuanglian preparation,a Chinese traditional patent medicine with a long history for treating respiratory tract infection in China.We showed that either the oral liquid of Shuanghuanglian,the lyophilized powder of Shuanghuanglian for injection or their bioactive components dose-dependently inhibited SARS-CoV-23CLpro as well as the replication of SARS-CoV-2 in Vero E6 cells.Baicalin and baicalein,two ingredients of Shuanghuanglian,were characterized as the first noncovalent,nonpeptidomimetic inhibitors of SARS-CoV-23CLpro and exhibited potent antiviral activities in a cell-based system.Remarkably,the binding mode of baicalein with SARS-CoV-23CLpro determined by X-ray protein crystallography was distinctly different from those of known 3CLpro inhibitors.Baicalein was productively ensconced in the core of the substrate-binding pocket by interacting with two catalytic residues,the crucial S1/S2 subsites and the oxyanion loop,acting as a“shield”in front of the catalytic dyad to effectively prevent substrate access to the catalytic dyad within the active site.Overall,this study provides an example for exploring the in vitro potency of Chinese traditional patent medicines and effectively identifying bioactive ingredients toward a specific target,and gains evidence supporting the in vivo studies of Shuanghuanglian oral liquid as well as two natural products for COVID-19 treatment. | Hai-xia Su Sheng Yao Wen-feng Zhao Min-jun Li Jia Liu Wei-juan Shang Hang Xie Chang-qiang Ke Hang-chen Hu Mei-na Gao Kun-qian Yu Hong Liu Jing-shan Shen Wei Tang Lei-ke Zhang Geng-fu Xiao Li Ni Dao-wen Wang Jian-ping Zuo Hua-liang Jiang Fang Bai Yan Wu Yang Ye Ye-chun Xu | 2020 | Acta Pharmacologica Sinica2020，41，9： | 32 |
| 6 | Future changes in thermal comfort conditions over China based on multi-RegCM4 simulations显示文摘使用4个不同全球模式模拟结果,驱动RegCM4区域气候模式,进行了RCP4.5中等温室气体排放情景下东亚地区21世纪气候变化的高分辨率(25 km)模拟。本文基于此套模拟集合,使用综合考虑了气温、相对湿度和风速因素的人体热感受指数有效温度(ET),给出了未来中国区域热舒适条件的变化。分析中同时考虑了未来人口数量和分布情景所引起的暴露度变化。研究发现,未来中国区域的ET的普遍升高引起炎热天气的人口暴露度大幅度增加(到21世纪末相对于当代全国平均将增加6倍‘人-天’(person–days),凉、冷和寒冷天气的暴露度则减少。同时尽管舒适日数有所增加,但相应的人-天数目则有22%的减少。不同因素(气候、人口和两者的相互作用)对于上述变化的贡献分析表明,在舒适度的热端,气候因素的作用更重要,而人口因素则在冷端起着主导作用。总体而言,即使是在中等水平温室气体排放情景下,中国区域未来的热强度也将增加,尽管结果表现出很强的地理依赖特征。相对使用单纯气候因素的结果,人口暴露度的引入对变化信号有着强烈的调制作用,从而更加强调了在与气候变化相关的风险评估分析中,考虑社会-经济因素的重要性。 | GAO Xue-Jie WU Jie SHI Ying WU Jia HAN Zhen-Yu ZHANG Dong-Feng TONG Yao LI Rou-Ke XU Ying GIORGI Filippo | 2018 | Atmospheric and Oceanic Science Letters2018，11，4： | 30 |
| 7 | A preventive control strategy for static voltage stability based on an efficient power plant model of electric vehicles显示文摘With the increasing integration of wind farms and electric vehicles(EVs)in power systems,voltage stability is becoming more and more serious.Based on vehicle-to-grid(V2G),an efficient power plant model of EVs(E-EPP)was developed to estimate EV charging load with available corresponding response capacity under different charging strategies.A preventive control strategy based on E-EPP was proposed to maintain the static voltage stability margin(VSM)of power system above a predefined security level.Two control modes were used including the disconnection of EV charging load(‘V1G’mode)and the discharge of stored battery energy back to power grid(‘V2G’mode).A modified IEEE 14-bus system with high penetration of wind power and EVs was used to verify the effectiveness of preventive control strategy.Simulation results showed that the proposed strategy can not only improve the static voltage stability of power system with considerable wind generation,but also guarantee the travelling comfort for EV owners. | Mingshen WANG Yunfei MU Hongjie JIA Jianzhong WU Xiuping YAO Xiaodan YU Janaka EKANAYAKE | 2015 | Journal of Modern Power Systems and Clean Energy2015，3，1： | 25 |
| 8 | Carbon peak and carbon neutrality in China:Goals,implementation path and prospects显示文摘Climate change is a common problem in human society.The Chinese government promises to peak carbon dioxide emissions by 2030 and strives to achieve carbon neutralization by 2060.The proposal of the goal of carbon peak and carbon neutralization has led China into the era of climate economy and set off a green change with both opportunities and challenges.On the basis of expounding the objectives and specific connotation of China’s carbon peak and carbon neutralization,this paper systematically discusses the main implementation path and the prospect of China’s carbon peak and carbon neutralization.China’s path to realizing carbon neutralization includes four directions:(1)in terms of carbon dioxide emission control:energy transformation path,energy conservation,and emission reduction path;(2)for increasing carbon sink:carbon capture,utilization,and storage path,ecological governance,and land greening path;(3)in key technology development:zero-carbon utilization,coal new energy coupling,carbon capture utilization and storage(CCUS),energy storage technology and other key technology paths required to achieve carbon peak and carbon neutralization;(4)from the angle of policy development:Formulate legal guarantees for the government to promote the carbon trading market;Formulate carbon emission standards for enterprises and increase publicity and education for individuals and society.Based on practicing the goal and path of carbon peak and carbon neutralization,China will vigorously develop low carbon and circular economy and promote green and high-quality economic development;speed up to enter the era of fossil resources and promoting energy transformation;accelerate the integrated innovation of green and low-carbon technologies and promote carbon neutrality. | Yao Wang Chi-hui Guo Xi-jie Chen Li-qiong Jia Xiao-na Guo Rui-shan Chen Mao-sheng Zhang Ze-yu Chen Hao-dong Wang | 2021 | China Geology2021，4，4： | 24 |
| 9 | Conversion to sirolimus immunosuppression in liver transplantation recipients with hepatocellular carcinoma:Report of an initial experience显示文摘瞄准:报导收到了 sirolimus 的 36 个病人的初步的结果的回顾的分析(SRL, Rapamune, rapamycin ) 在 248 肝的一个连续的队紧密相联的接枝接受者。方法:被换到的有肝细胞癌(HCC ) 的 36 个肝 transplant 病人从 tacrolimus 的基于 SRL 的免疫力的抑制治疗在这研究被注册。是在常位的肝移植( OLT )前推进了 HCC 被诊断的病人被划分成组 A ( n = 11 ),在 OLT 被分配组织 B 以后,被发现到的那些有 HCC 复发或转移( n = 18 ),并且 calcineurin 禁止者( CNI )引起的发达肾机能不全被分到谁的那些组织 C ( n = 7 )在 OLT 以后。结果:病人被跟随在上面为一 10.4 瞬间中部(范围, 3.8-19.1 瞬间) 在到 SRL 治疗和 12.3 瞬间的变换以后(范围, 5.1-34.4 瞬间) 在 OLT 以后。开发的三个病人在开始 SRL 治疗以后的 2 wk,它是充分在氢化尼松以后颠倒了的温和尖锐细胞的拒绝搏动治疗。在组 A,仅仅 1 个病人被发现有 HCC 复发和转移在 OLT 以后的 12 瞬间。在组 B, 66.7%(12/18 ) 病人们(2 与进步肿瘤, 7 与稳定的肿瘤并且 3 没有肿瘤) 由于在 6.8 瞬间柱子变换和 10.7 瞬间柱子 transplant 的中部的后续的结束为 HCC 复发交谈到 SRL 或切除术仍然是活着的。在组 C,没有 HCC 复发在 7 个病人被表明,并且肾的功能在 SRL 治疗以后变得正常。血小板减少(n = 2 ) ,贫血症(n = 8 ) ,并且口头的 aphthous (n = 7 ) 在我们的队发现是容易可管理的。结论:到基于 SRL 的免疫力的抑制的变换可以禁止 HCC 的复发和转移并且与 HCC 在 OLT 病人改进导致 CNI 的肾机能不全。 | Jian Zhou Jia Fan Zheng Wang Zhi-Quan Wu Shuang-Jian Qiu Xiao-Wu Huang Yao Yu Jian Sun Yong-Sheng Xiao Yi-Feng He Yu-Qi Wang Zhao-You Tang | 2006 | World Journal of Gastroenterology2006，12，19： | 20 |
| 10 | Effect of emodin and sandostatin on metabolism of eicosanoids in acute necrotizing pancreatitis显示文摘INTRODUCTIONIn order to study the therapeutic mechanisms ofemodin,an extract of Rhubarb (Rhizoma et RadixRhei,a traditional Chinese herbal medicine),andsandostatin in the treatment of acute necrotizingpancreatitis (ANP),we used the two drugs in ratmodels of the disease and observed the changes ofplasma thromboxane-2(TXB2),6-keto-prostaglandin F1α(6-keto-PGF1α)and prostaglandinE2(PEG2). | Jian Xin Wu Jia Yu Xu Yao Zong Yuan | 2000 | World Journal of Gastroenterology2000，6，2： | 19 |
| 11 | Proton magnetic resonance spectroscopy in the evaluation of infiltration zone of cerebral alveolar echinococcosis显示文摘 | WANG Jian YAO Wei-hong YI Ba-nu LIU Shen ZHANG De-qing JIANG Chun-hui ZHANG Li-min NIU Jun-qiao JIA Wen-xiao WEN Hao | 2012 | Chinese Medical Journal2012，，13： | 19 |
| 12 | Efficacy and safety of metformin and sitagliptin based triple antihyperglycemic therapy(STRATEGY):a multicenter,randomized,controlled,non-inferiority clinical trial显示文摘Despite the current guideline's recommendation of a timely stepwise intensification therapy,the 'clinical inertia',termed as the delayed treatment intensification,commonly exists in the real world,which may be partly due to the relatively little substantial evidence and no clear consensus regarding the efficacy and safety of triple oral agents in patients inadequately controlled with dual therapy.In this clinical trial performed in 237 centers in China,5,535 type 2 diabetic patients inadequately controlled by previous therapies were treated with a stable metformin/sitagliptin dual therapy for 20 weeks.The patients who did not reach the glycated hemoglobin A1c(HbA1c) goal were then further randomized into glimepiride,gliclazide,repaglinide,or acarbose group for an additional 24-week triple therapy.A mean HbAlc reduction of 0.85%was observed when sitagliptin was added to the patients inadequately controlled with metformin in 16 weeks.Further HbAlc reductions in the 24-week triple therapy stage were 0.65%in glimepiride group,0.70%in gliclazide group,0.61%in repaglinide group,and 0.45%in acarbose group.The non-inferiority criterion for primary hypotheses was met for gliclazide and repaglinide,but not for acarbose,compared with glimepiride,when added to metformin/sitagliptin dual therapy.The incidences of adverse events(AEs) were 29.2%in the dual therapy stage and30.3%in the triple therapy stage.Metformin/sitagliptin as baseline therapy,with the addition of a third oral antihyperglycemic agent,including glimepiride,gliclazide,repaglinide,or acarbose,was effective,safe and well-tolerated for achieving an HbAlc<7.0%goal in type 2 diabetic patients inadequately controlled with previous therapies.The timely augmentation of up to three oral antihyperglycemic agents is valid and of important clinical benefit to prevent patients from exposure to unnecessarily prolonged hyperglycemia. | Wen Xu Yiming Mu Jiajun Zhao Dalong Zhu Qiuhe Ji Zhiguang Zhou Bin Yao Anhua Mao Samuel S.Engel Bin Zhao Yan Bi Longyi Zeng Xingwu Ran Juming Lu Linong Ji Wenying Yang Weiping Jia Jianping Weng | 2017 | Science China(Life Sciences)2017，60，3： | 19 |
| 13 | Infection with novel coronavirus(SARS-CoV-2)causes pneumonia in Rhesus macaques显示文摘The 2019 novel coronavirus(SARS-CoV-2)outbreak is a major challenge for public health.SARS-CoV-2 infection in human has a broad clinical spectrum ranging from mild to severe cases,with a mortality rate of^6.4%worldwide(based on World Health Organization daily situation report).However,the dynamics of viral infection,replication and shedding are poorly understood.Here,we show that Rhesus macaques are susceptible to the infection by SARS-CoV-2.After intratracheal inoculation,the first peak of viral RNA was observed in oropharyngeal swabs one day post infection(1 d.p.i.),mainly from the input of the inoculation,while the second peak occurred at 5 d.p.i.,which reflected on-site replication in the respiratory tract.Histopathological observation shows that SARS-CoV-2 infection can cause interstitial pneumonia in animals,characterized by hyperemia and edema,and infiltration of monocytes and lymphocytes in alveoli.We also identified SARS-CoV-2 RNA in respiratory tract tissues,including trachea,bronchus and lung;and viruses were also re-isolated from oropharyngeal swabs,bronchus and lung,respectively.Furthermore,we demonstrated that neutralizing antibodies generated from the primary infection could protect the Rhesus macaques from a second-round challenge by SARS-CoV-2.The non-human primate model that we established here provides a valuable platform to study SARS-CoV-2 pathogenesis and to evaluate candidate vaccines and therapeutics. | Chao Shan Yan-Feng Yao Xing-Lou Yang Yi-Wu Zhou Ge Gao Yun Peng Lian Yang Xue Hu Jin Xiong Ren-Di Jiang Hua-Jun Zhang Xiao-Xiao Gao Cheng Peng Juan Min Ying Chen Hao-Rui Si Jia Wu Peng Zhou Yan-Yi Wang Hong-Ping Wei Wei Pang Zheng-Fei Hu Long-Bao Lv Yong-Tang Zheng Zheng-Li Shi Zhi-Ming Yuan | 2020 | Cell Research2020，30，8： | 19 |
| 14 | Combined acoustic radiation force impulse, aminotransferase to platelet ratio index and Forns index assessment for hepatic fibrosis grading in hepatitis B显示文摘AIM: To investigate the combined diagnostic accuracy of acoustic radiation force impulse(ARFI), aspartate aminotransferase to platelet ratio index(APRI) and Forns index for a non-invasive assessment of liver fibrosis in patients with chronic hepatitis B(CHB). METHODS: In this prospective study, 206 patients had CHB with liver fibrosis stages F0-F4 classified by METAVIR and 40 were healthy volunteers were measured by ARFI, APRI and Forns index separately or combined as indicated. RESULTS: ARFI, APRI or Forns index demonstrated a significant correlation with the histological stage(all P < 0.001). According to the AUROC of ARFI and APRI for evaluating fibrotic stages more than F2, ARFI showed an enhanced diagnostic accuracy than APRI(P < 0.05). The combined measurement of ARFI and APRI exhibited better accuracy than ARFI alone when evaluating ≥ F2 fibrotic stage(Z = 2.77, P = 0.006). Combination of ARFI, APRI and Forns index did not obviously improve the diagnostic accuracy compared to the combination of ARFI and APRI(Z = 0.958, P = 0.338). CONCLUSION: ARFI + APRI showed enhanced diagnostic accuracy than ARFI or APRI alone for significant liver fibrosis and ARFI + APRI + Forns index shows the same effect with ARFI + APRI. | Chang-Feng Dong Jia Xiao Ling-Bo Shan Han-Ying Li Yong-Jia Xiong Gui-Lin Yang Jing Liu Si-Min Yao Sha-Xi Li Xiao-Hua Le Jing Yuan Bo-Ping Zhou George L Tipoe Ying-Xia Liu | 2016 | World Journal of Hepatology2016，8，14： | 18 |
| 15 | Diurnal variation of number concentration and size distribution of ultrafine particles in the urban atmosphere of Beijing in winter显示文摘在尺寸的在空中的粒子的数字集中和分发变化 5.6 ~ 560 nm 直径在冬季 2005 为一个 15-d 时期在北京被测量。成核模式(5.6 20 nm ) 的每天平均的数字集中,艾特肯模式(20 100 nm ) ,和累积模式(100 560 nm ) 粒子,和全部的粒子是 17500, 32000, 4000,和 53500 厘米(? 3 ) 分别地。平均颗粒大小分发是单音的与在夜里和与大约 10 和大约 40 nm 的模式直径形式的双性人的大约 40 nm 的一条模式直径形式在白天期间。新粒子形成事件,它被连接到日报成核模式粒子的变化,被观察在多于观察天的一半。事件经常在 10:00 11:00 汉语标准时间(CST ) 附近开始了并且在 3 4 h 以后结束了。艾特肯和累积模式粒子的集中从午夜增加了并且在大约 10:00 CST 到达了他们的最大值,然后减少并且在下午变得最低。分析日报在交通体积和气象学的参数的周期表明在艾特肯的粒子和在早上的累积模式的累积被在车辆排放的倒置和增加的形成影响,并且在下午的如此的粒子的分散与更有效的垂直混合和更高的风速度被联系。 | SHI Zong-bo HE Ke-bin YU Xue-chun YAO Zhi-liang YANG Fu-mo MA Yong-liang MA Rui JIA Ying-tao ZHANG Jie | 2007 | Journal of Environmental Sciences2007，19，8： | 16 |
| 16 | Downstaging followed by resection plays a role in improving prognosis of unresectable hepatocellular carcinoma显示文摘BACKGROUND: Curable outcome of unresectable hepato- cellular carcinoma ( HCC) was seldom encountered in the past. This study was designed to assess the role of down- staging followed by resection ( downstaging-resection) in the improvement of prognosis of unresectable HCC. METHODS: During the period of 1958-2003 , a total of 1085 patients were verified surgically to be unresectable. Of these patients, 139 received downstaging-resection, with a rate of 84.2% for coexisting cirrhosis and a median tumor diame- ter of 11.1 cm. Resection of the right lobe, hepatic hilum and bilateral cancer accounted for 97. 8% of the patients. Downstaging including hepatic artery ligation ( HAL) + he- patic artery chemo-infusion ( HAI ) was performed in 65.5% of the patients, HAL + HAI + radiotherapy/radioim- munotherapy in 29. 5%, and HAL or HAI alone in 5.0%. Retrospective analysis was made of the survival of patients with unresectable HCC, downstaging-resection rate and treatment pattern. RESULTS: In the 139 patients with downstaging-resection, the median interval between the first and second operation was 7.2 months and the 5-year survival rate calculated from the first operation was 48. 7%. In the 1085 patients with un- resectable HCC, their 5-year survival was 0% in the period of 1958-1973, 11.5% in the period of 1974-1988 and 19.3% in the period of 1989-2003. These figures were correlated with the increasing downstaging-resection rate from 0%, 9.0% to 15.6%, and the increasing percentage of triple or double combination treatment from 32.2%, 60.4% to 69.7%. The 5-year survival in triple treatment group was 24. 9%, double treatment 15.2%, and single treatment only 10.9%, which was also correlated with the downstaging-re- section rate of 34.6%, 16.2% and 1.8% respectively. CONCLUSIONS: Downstaging-resection plays a role in improving prognosis of unresectable HCC. Triple and double treatments provide a higher downstaging-resection rate and may result in better prognosis. | Zhao-You Tang,Xin-Da Zhou, Zeng-Chen Ma, Zhi-Quan Wu, Jia Fan, Lun-Xiu Qin and Yao Yu Shanghai, China Liver Cancer Institute & Zhongshan Hospital, Fudan University, Shanghai 200032 , China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，4： | 16 |
| 17 | Exosomes derived from bone marrow mesenchymal stem cells protect the injured spinal cord by inhibiting pericyte pyroptosis显示文摘Mesenchymal stem cell(MSC)transplantation is a promising treatment strategy for spinal cord injury,but immunological rejection and possible tumor formation limit its application.The therapeutic effects of MSCs mainly depend on their release of soluble paracrine factors.Exosomes are essential for the secretion of these paracrine effectors.Bone marrow mesenchymal stem cell-derived exosomes(BMSC-EXOs)can be substituted for BMSCs in cell transplantation.However,the underlying mechanisms remain unclear.In this study,a rat model of T10 spinal cord injury was established using the impact method.Then,30 minutes and 1 day after spinal cord injury,the rats were administered 200μL exosomes via the tail vein(200μg/mL;approximately 1×106 BMSCs).Treatment with BMSC-EXOs greatly reduced neuronal cell death,improved myelin arrangement and reduced myelin loss,increased pericyte/endothelial cell coverage on the vascular wall,decreased bloodspinal cord barrier leakage,reduced caspase 1 expression,inhibited interleukin-1βrelease,and accelerated locomotor functional recovery in rats with spinal cord injury.In the cell culture experiment,pericytes were treated with interferon-γand tumor necrosis factor-α.Then,Lipofectamine 3000 was used to deliver lipopolysaccharide into the cells,and the cells were co-incubated with adenosine triphosphate to simulate injury in vitro.Pre-treatment with BMSC-EXOs for 8 hours greatly reduced pericyte pyroptosis and increased pericyte survival rate.These findings suggest that BMSC-EXOs may protect pericytes by inhibiting pyroptosis and by improving blood-spinal cord barrier integrity,thereby promoting the survival of neurons and the extension of nerve fibers,and ultimately improving motor function in rats with spinal cord injury.All protocols were conducted with the approval of the Animal Ethics Committee of Zhengzhou University on March 16,2019. | Yan Zhou Lu-Lu Wen Yan-Fei Li Kai-Min Wu Ran-Ran Duan Yao-Bing Yao Li-Jun Jing Zhe Gong Jun-Fang Teng Yan-Jie Jia | 2022 | Neural Regeneration Research2022，17，1： | 15 |
| 18 | Liver Enzymes Concentrations Are Closely Related to Pre-diabetes:Findings of the Shanghai Diabetes Study Ⅱ (SHDS Ⅱ)显示文摘Objective To investigate the relationship of liver enzymes with hyperglycemia in a large population in Shanghai and identify the association between liver enzymes and insulin resistance.Methods A total of 3 756 participants were enrolled.Each participant underwent an oral glucose tolerance test and completed a questionnaire.Anthropometric indices were recorded and serum samples were collected for measurement.Results Liver enzymes concentrations were independently associated with i-IGT,IFG+IGT,and diabetes.With the increase of ALT and GGT concentrations,ORs for i-IGT,IFG+IGT,and diabetes increased gradually.By comparing patients in the highest quartile of GGT concentrations or ALT concentrations with those in the lowest quartile (Q1),ORs for i-IGT,IFG+IGT,or diabetes was significant after adjustment.Both ALT and GGT concentrations were linearly correlated with HOMA-IR and independently associated with HOMA-IR [ALT OR (95% CI):2.56 (1.51-4.34) P=0.00;GGT OR (95% CI):2.66 (1.53-4.65) P=0.00].Conclusion Serum ALT and GGT concentrations were closely related to pre-diabetes and diabetes in the Shanghai population and positively associated with insulin resistance. | GAO Fei PAN Jie Min HOU Xu Hong FANG Qi Chen LU Hui Juan TANG Jun Ling GU Hui Lin PAN Zhi Jian YAO You Hua SHEN Wei Zhen JIA Wei Ping | 2012 | Biomedical and Environmental Sciences2012，25，1： | 15 |
| 19 | miR-34a mediates oxaliplatin resistance of colorectal cancer cells by inhibiting macroautophagy via transforming growth factor-β/Smad4 pathway显示文摘AIM To investigate whether micro RNA(mi R)-34 a mediates oxaliplatin(OXA) resistance of colorectal cancer(CRC) cells by inhibiting macroautophagy via the transforming growth factor(TGF)-β/Smad4 pathway.METHODS miR-34 a expression levels were detected in CRC tissues and CRC cell lines by quantitative real-time polymerase chain reaction. Computational search, functional luciferase assay and western blotting were used to demonstrate the downstream target of miR-34 a in CRC cells. Cell viability was measured with Cell Counting Kit-8. Apoptosis and macroautophagy of CRC cells were analyzed by flow cytometry and transmission electron microscopy, and expression of beclin I and LC3-II was detected by western blotting.RESULTS Expression of miR-34 a was significantly reduced while expression of TGF-β and Smad4 was increased in CRC patients treated with OXA-based chemotherapy. OXA treatment also resulted in decreased mi R-34 a levels and increased TGF-β and Smad4 levels in both parental cells and the OXA-resistant CRC cells. Activation of macroautophagy contributed to OXA resistance in CRC cells. Expression levels of Smad4 and miR-34 a in CRC patients had a significant inverse correlation and overexpressing mi R-34 a inhibited macroautophagy activation by directly targeting Smad4 through the TGF-β/Smad4 pathway. OXA-induced downregulation of miR-34 a and increased drug resistance by activating macroautophagy in CRC cells.CONCLUSION miR-34 a mediates OXA resistance of CRC by inhibiting macroautophagy via the TGF-β/Smad4 pathway. | Chen Sun Fu-Jing Wang Hao-Gang Zhang Xun-Zheng Xu Rui-Chun Jia Lei Yao Peng-Fei Qiao | 2017 | World Journal of Gastroenterology2017，23，10： | 15 |
| 20 | Antioxidant and Anti-inflammatory Capacity of Ferulic Acid Released from Wheat Bran by Solid-state Fermentation of Aspergillus niger显示文摘Objective A strain of Aspergillus niger(A. niger), capable of releasing bound phenolic acids from wheat bran, was isolated. This strain was identified by gene sequence identification. The antioxidant and anti-inflammatory capacity of ferulic acid released from wheat bran by this A. niger strain(FA-WB) were evaluated. Methods Molecular identification techniques based on PCR analysis of specific genomic sequences were conducted; antioxidant ability was examined using oxygen radical absorbance capacity(ORAC), cellular antioxidant activity(CAA) assays, and erythrocyte hemolysis assays. RAW264.7 cells were used as a model to detect anti-inflammatory activity. Results The filamentous fungal isolate was identified to be A. niger. ORAC and CAA assay showed that FA-WB had better antioxidant activity than that of the ferulic acid standard. The erythrocyte hemolysis assay results suggested that FA-WB could attenuate AAPH-induced oxidative stress through inhibition of reactive oxy gen species(ROS) generation. FA-WB could significantly restore the AAPH-induced increase in intracellular antioxidant enzyme activities to normal levels as well as inhibit the intracellular malondialdehyde formation. TNF-?, IL-6, and NO levels indicated that FA-WB can inhibit the inflammation induced by lipopolysaccharide(LPS). Conclusion Ferulic acid released from wheat bran by a new strain of A. niger had good anti-inflammatory activity and better antioxidant ability than standard ferulic acid. | YIN Zhi Na WU Wen Jia SUN Chong Zhen LIU Hui Fan CHEN Wen Bo ZHAN Qi Ping LEI Zhuo Gui XIN Xuan MA Juan Juan YAO Kun MIN Tian ZHANG Meng Meng WU Hui | 2019 | Biomedical and Environmental Sciences2019，32，1： | 14 |
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