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| 1 | Molecular architecture of the luminal ring of the Xenopus laevis nuclear pore complex显示文摘The nuclear pore complex(NPC)mediates the flow of substances between the nucleus and cytoplasm in eukaryotic cells.Here we report the cryo-electron tomography(cryo-ET)structure of the luminal ring(LR)of the NPC from Xenopus laevis oocyte.The observed key structural features of the LR are independently confirmed by single-particle cryo-electron microscopy(cryo-EM)analysis.The LR comprises eight butterfly-shaped subunits,each containing two symmetric wings.Each wing consists of four elongated,tubular protomers.Within the LR subunit,the eight protomers form a Finger domain,which directly contacts the fusion between the inner and outer nuclear membranes and a Grid domain,which serves as a rigid base for the Finger domain.Two neighboring LR subunits interact with each other through the lateral edges of their wings to constitute a Bumper domain,which displays two major conformations and appears to cushion neighboring NPCs.Our study reveals previously unknown features of the LR and potentially explains the elastic property of the NPC. | Yanqing Zhang Sai Li Chao Zeng Gaoxingyu Huang Xuechen Zhu Qifan Wang Kunpeng Wang Qiang Zhou Chuangye Yan Wusheng Zhang Guangwen Yang Minhao Liu Qinghua Tao Jianlin Lei Yigong Shi | 2020 | Cell Research2020，30，6： | 4 |
| 2 | Wet Desulfurization of High-Sulfur Petroleum Coke Improved via Pre-calcination,H_(2)O_(2) Treatment,and Ultrasound显示文摘This work reports an improved method for the wet desulfurization of high-sulfur petroleum coke(petcoke)powder based on the combination of pre-calcination,H_(2)O_(2),and ultrasound.The results demonstrated that over 45%of the sulfur atoms were efficiently removed from Tianjin coke and Qilu coke(particle size<0.1 mm)by pre-calcination at 800℃ for 6 h followed by desulfurization with HNO3(8 mol/L)and H_(2)O_(2)(2 mol/L)solution at a reaction temperature of 60℃,a reaction time of 6 h,a liquid-to-solid ratio of 10 mL/g,and a 40 kHz ultrasonic power of 400 W.In addition,the specific surface area of the petcoke particles increased from 0.7 to 301.49 m^(2)/g.After desulfurization,the pore size distribution of the petcoke particles was more concentrated on micropores compared with the samples prior to petcoke treatment.Reactive force field molecular dynamics simulation results indicated that HNO_(3) continuously oxidized the carbon atoms adjacent to sulfur atoms in the petcoke macromolecules and promoted sulfur removal from petcoke via the cleavage of C-S bonds.The sulfur transformation mechanism can be summarized as follows:thiophene sulfur→branched chain carbon sulfur→CO_(2)S→C_(2)O_(2)S→C_(2)O_(3)NS→C_(2)O_(4)S→CO_(2)S. | Wang Gang Mao Qiuyun Liu Wei Yu Zhou Zhong Qifan | 2022 | China Petroleum Processing & Petrochemical Technology2022，24，2： | 3 |
| 3 | TGF-β1-supplemented decellularized annulus fibrosus matrix hydrogels promote annulus fibrosus repair显示文摘Annulus fibrosus(AF)repair remains a challenge because of its limited self-healing ability.Endogenous repair strategies combining scaffolds and growth factors show great promise in AF repair.Although the unique and beneficial characteristics of decellularized extracellular matrix(ECM)in tissue repair have been demonstrated,the poor mechanical property of ECM hydrogels largely hinders their applications in tissue regeneration.In the present study,we combined polyethylene glycol diacrylate(PEGDA)and decellularized annulus fibrosus matrix(DAFM)to develop an injectable,photocurable hydrogel for AF repair.We found that the addition of PEGDA markedly improved the mechanical strength of DAFM hydrogels while maintaining their porous structure.Transforming growth factor-β1(TGF-β1)was further incorporated into PEGDA/DAFM hydrogels,and it could be continuously released from the hydrogel.The in vitro experiments showed that TGF-β1 facilitated the migration of AF cells.Furthermore,PEGDA/DAFM/TGF-β1 hydrogels supported the adhesion,proliferation,and increased ECM production of AF cells.In vivo repair performance of the hydrogels was assessed using a rat AF defect model.The results showed that the implantation of PEGDA/DAFM/TGF-β1 hydrogels effectively sealed the AF defect,prevented nucleus pulposus atrophy,retained disc height,and partially restored the biomechanical properties of disc.In addition,the implanted hydrogel was infiltrated by cells resembling AF cells and well integrated with adjacent AF tissue.In summary,findings from this study indicate that TGF-β1-supplemented DAFM hydrogels hold promise for AF repair. | Qiang Wei Dachuan Liu Genglei Chu Qifan Yu Zhao Liu Jiaying Li Qingchen Meng Weishan Wang Fengxuan Han Bin Li | 2023 | Bioactive Materials2023，，1： | 3 |
| 4 | Fabrication and characterization of 3D printed biocomposite scaffolds based on PCL and zirconia nanoparticles显示文摘The application of three-dimensional printed polymer scaffolds in repairing bone defects is a promising strategy.Among them,polycaprolactone(PCL)scaffolds are widely studied due to their good processability and controlled degradation rate.However,as an alternative graft for repairing bone defects,PCL materials have poor hydrophilicity,which is not conducive to cell adhesion and growth.In addition,the poor mechanical properties of PCL materials cannot meet the strength required to repair bone defects.In this paper,nano-zirconium dioxide(ZrO2)powder is embedded in PCL material through a meltmixing process,and a regular grid scaffold is constructed by 3D printing.The embedding of nanometer zirconium dioxide powder improves the hydrophilicity and water absorption of the composite scaffold,which is conducive to cell adhesion,proliferation and growth and is beneficial to the exchange of nutrients.Therefore,the PCL/ZrO2 composite scaffold showed better biological activity in vitro.At the same time,the PCL/ZrO2 composite material system significantly improves the mechanical properties of the scaffold.Among them,compared with the pure PCL scaffold,the Young’s modulus is increased by about 0.4 times,and the compressive strength is increased by about 0.5 times.In addition,the osteogenic differentiation results also showed that the PCL/ZrO2 composite scaffold group showed better ALP activity and more effective bone mineralization than the pure PCL group.We believe that the 3D printed PCL/ZrO2 composite scaffold has certain application prospects in repairing bone defects. | Qifan Wang Zhiyong Ma Ying Wang Linna Zhong Wenjia Xie | 2021 | Bio-Design and Manufacturing2021，4，1： | 2 |
| 5 | Structure of the cytoplasmic ring of the Xenopus laevis nuclear pore complex by cryo-electron microscopy single particle analysis显示文摘The nuclear pore complex(NPC)exhibits structural plasticity and has only been characterized at local resolutions of up to 15 A for the cytoplasmic ring(CR).Here we present a single-particle cryo-electron microscopy(cryo-EM)structure of the CR from Xenopus laevis NPC at average resolutions of 5.5-7.9A,with local resolutions reaching 4.5 A.Improved resolutions allow identification and placement of secondary structural elements in the majority of the CR components.The two Y complexes in each CR subunit interact with each other and associate with those from flanking subunits,forming a circular scaffold.Within each CR subunit,the Nup358-containing region wraps around the stems of both Y complexes,likely stabilizing the scaffold.Nup205 connects the short arms of the two Y complexes and associates with the stem of a neighboring Y complex.The Nup214-containing region uses an extended coiled-coil to link Nup85 of the two Y complexes and protrudes into the axial pore of the NPC.These previously uncharacterized structural features reveal insights into NPC assembly. | Gaoxingyu Huang Yanqing Zhang Xuechen Zhu Chao Zeng Qifan Wang Qiang Zhou Qinghua Tao Minhao Liu Jianlin Lei Chuangye Yan Yigong Shi | 2020 | Cell Research2020，30，6： | 2 |
| 6 | Cryo-EM structure of the inner ring from the Xenopus laevis nuclear pore complex显示文摘Nuclear pore complex(NPC)mediates nucleocytoplasmic shuttling.Here we present single-particle cryo-electron microscopy structure of the inner ring(IR)subunit from the Xenopus laevis NPC at an average resolution of 4.2Å.A homo-dimer of Nup205 resides at the center of the IR subunit,flanked by two molecules of Nup188.Four molecules of Nup93 each places an extended helix into the axial groove of Nup205 or Nup188,together constituting the central scaffold.The channel nucleoporin hetero-trimer of Nup62/58/54 is anchored on the central scaffold.Six Nup155 molecules interact with the central scaffold and together with the NDC1–ALADIN hetero-dimers anchor the IR subunit to the nuclear envelope and to outer rings.The scarce inter-subunit contacts may allow sufficient latitude in conformation and diameter of the IR.Our structure reveals the molecular basis for the IR subunit assembly of a vertebrate NPC. | Gaoxingyu Huang Xiechao Zhan Chao Zeng Ke Liang Xuechen Zhu Yanyu Zhao Pan Wang Qifan Wang Qiang Zhou Qinghua Tao Minhao Liu Jianlin Lei Chuangye Yan Yigong Shi | 2022 | Cell Research2022，32，5： | 1 |
| 7 | High-resolution linkage and quantitative trait locus mapping using an interspecific cross between Argopecten irradians irradians(♀) and A. purpuratus (♂)显示文摘The bay scallop and Peruvian scallop are economically important species.Interspecific hybrids of these two scallops outperformed both of their parent species in multiple growth traits but exhibited decreased fertility,which provides good models for the study of heterosis and species divergence.Genetic mapping serves as a chromosomal-level framework to investigate the molecular mechanisms of hybridization and introgression.In this study,high-resolution linkage maps were constructed for the bay and Peruvian scallops with an interspecific hybrid family.The linkage map of the bay scallop covered over 98.9% of the whole genome with 2994 mapped markers and the average marker interval of 0.32 cM.For the Peruvian scallop,1585 markers were mapped with the average maker interval of 0.51 cM,covering 97.7% of the genome.Both the two linkage maps have 16 linkage groups,corresponding to the haploid chromosome number of the two species.Approximately,54.5% of markers exhibited significant deviation from the expected Mendelian ratio of segregation,lending in sights into the intrinsic incompatibility between the two species.QTLs related to growth and shell coloration were detected,which could explain 13.1%and 74.9% of the phenotypic variance,respectively.This represents important information for further evaluation.These findings are an important addition to the genomic resources for scallop genetic studies,and are especially useful for investigations on genomic incompatibility for hybridization,genome evolution of closely related species,and genetic enhancement programs in aquaculture. | Junxia Mao Qifan Zeng Zujing Yang Haoyuan Pan Lijie Yao Zhenmin Bao Chunde Wang Shi Wang | 2020 | Marine Life Science & Technology2020，2，2： | 1 |
| 8 | Enhanced Microwave Absorption for High Filler Content Composite Molded from Polymer Coated Flaky Carbonyl Irons Modified by Silane Coupling Agents显示文摘A microwave absorbing sheet with a high complex permeability and a relatively low complex permittivity is obtained by molding of the densely coated flaky carbonyl iron particles(FCIPs)by styrenebutadiene-styrene block copolymer(SBS)in the assistance of coupling agent modification.Direct molding of the core-shell FCIPs without adding extra binder results in a large permeability due to the high filling ratio(55vol%)of absorbents.Importantly,the permittivity is well suppressed by the dense insulate polymer shell on the FCIPs,avoiding the severe impedance mismatch problem of the high filler content microwave absorbing materials.Investigations show that modifying the surface of FCIPs by proper amount of silane coupling agent is critical for the coating quality of the SBS shell,which is verified by resistivity and corrosion current density measurements,and can be interpreted by improved interfacial compatibility between the modified FCIPs and SBS.The obtained microwave absorbing sheet shows a minimum reflection loss of-38.74 dB at 1.57 GHz and has an effective absorption bandwidth from 1.1 to 2.3 GHz at a relatively small thickness of 2 mm. | 崔正明 MA Guoqing WANG Mengqi LUO Chuangyu CHEN Zhihong MA Huiru LI Qifan 李维 | 2023 | Journal of Wuhan University of Technology(Materials Science)2023，38，1： | 1 |
| 9 | A novel dynamic approach of accelerometer calibration in GPS/DR integrated navigation system 显示文摘 | Wang Yanran Zhang Hai Zhou Qifan | 2013 | Applied Mechanics and Materials: Industrial Instrumentation and Control Systems2013，，： | 1 |
| 10 | Heterogeneous chemistry of trace atmospheric gases on atmospheric aerosols:An overview显示文摘Due to altering the budget of important trace gases and changing the composition of atmospheric aerosols,heterogeneous reactions between trace gases and liquid/solid aerosols have become an important subject of atmospheric science in recent years.However,the kinetics and mechanism of these reactions have not been well-understood yet.In recent years,we have established an integrated method to study a series of atmospheric heterogeneous reactions under control atmospheric conditions.Using on-line techniques,such as Diffuse Reflectance Infrared Fourier Transform spectroscopy,Raman microspectrometry and Knudsen cell,the heterogeneous reactions of a series of trace gases(NO2,SO2,H2O2,DMS,C2H5 I and Monocarboxylic acids)on mineral oxides and black carbon were investigated.The uptake coefficients and mechanisms at different atmospheric conditions(temperature,relative humidity and reactant concentration)were obtained.In addition,a home-made equipment named rotated wetted-wall reactor was designed for the in situinvestigations of the uptake of volatile organic compounds into the H2SO4 solution and H2SO4/H2O2 mixed solution.In this review,we present the main results obtained in our laboratory studies and propose some challenges that still exist in the heterogeneous reaction studies. | GE MaoFa WU LingYan TONG ShengRui LIU QiFan WANG WeiGang | 2015 | Science Foundation in China2015，23，3： | 1 |
| 11 | Direct and indirect immunolabelling of HeLa ceils with quantum dots 显示文摘 | Dongzhi Yang Qifan Chen Wenxing Wang | 2008 | Luminescence2008，23，： | 1 |
| 12 | The influence of schedule targeton project performance 显示文摘 | WANG QIFAN NING XIAOQIAN | 2003 | Journal of Fudan University: Natural Sci-ence2003，42，5： | 1 |
| 13 | Human ether-à-go-go-related gene expression is essential for cisplatinto induce apoptosis in human gastric cancer显示文摘 | Riping Zhang Pei Tian Qiang Chi Jizhou Wang Yongwei Wang Lingyu Sun Yang Liu Suli Tian Qifan Zhang | 2012 | Oncology Reports2012，，2： | 1 |
| 14 | Ribosome Profiling Reveals Genome-wide Cellular Translational Regulation upon Heat Stress in Escherichia coli显示文摘Heat shock response is a classical stress-induced regulatory system in bacteria, characterized by extensive transcriptional reprogramming. To compare the impact of heat stress on the transcriptome and translatome in Escherichia coli, we conducted ribosome profiling in parallel with RNA-Seq to investigate the alterations in transcription and translation efficiency when E. coli cells were exposed to a mild heat stress(from 30 °C to 45 °C). While general changes in ribosome footprints correlate with the changes of mRNA transcripts upon heat stress, a number of genes show differential changes at the transcription and translation levels. Translation efficiency of a few genes that are related to environment stimulus response is up-regulated, and in contrast, some genes functioning in mRNA translation and amino acid biosynthesis are down-regulated at the translation level in response to heat stress. Moreover, our ribosome occupancy data suggest that in general ribosomes accumulate remarkably in the starting regions of ORFs upon heat stress. This study provides additional insights into bacterial gene expression in response to heat stress, and suggests the presence of stress-induced but yet-to-be characterized cellular regulatory mechanisms of gene expression at translation level. | yanqing zhang zhengtao xiao qin zou jianhuo fang qifan wang xuerui yang ning gao | 2017 | Genomics, Proteomics & Bioinformatics2017，15，5： | 1 |
| 15 | Acid-catalyzed heterogeneous reaction of 3-methyl-2-buten-1-ol with hydrogen peroxide显示文摘Acid-catalyzed heterogeneous oxidation with hydrogen peroxide(H2O2) has been suggested to be a potential pathway for secondary organic aerosol(SOA) formation from isoprene and its oxidation products. However, knowledge of the chemical mechanism and kinetics for this process is still incomplete. 3-Methyl-2-buten-1-ol(MBO321), an aliphatic alcohol structurally similar to isoprene, is emitted by pine forests and widely used in the manufacturing industries. Herein the uptake of MBO321 into H2SO4–H2O2mixed solution was investigated using a flow-tube reactor coupled to a mass spectrometer. The reactive uptake coefficients(γ) were acquired for the first time and were found to increase rapidly with increasing acid concentration. Corresponding aqueous-phase reactions were performed to further study the mechanism of this acid-catalyzed reaction. MBO321 could convert to 2-methyl-3-buten-2-ol(MBO232) and yield isoprene in acidic media. Organic hydroperoxides(ROOHs) were found to be generated through the acid-catalyzed route,which could undergo a rearrangement reaction and result in the formation of acetone and acetaldehyde. Organosulfates, which have been proposed to be SOA tracer compounds in the atmosphere, were also produced during the oxidation process. These results suggest that the heterogeneous acid-catalyzed reaction of MBO321 with H2O2 may contribute to SOA mass under certain atmospheric conditions. | Qifan Liu Weigang Wang Maofa Ge | 2015 | Journal of Environmental Sciences2015，27，5： | 1 |
| 16 | Discrete Optimization on Unsteady Pressure Fluctuation of a Centrifugal Pump Using ANN and Modified GA显示文摘Pressure fluctuation due to rotor-stator interaction in turbomachinery is unavoidable,inducing strong vibration in the equipment and shortening its lifecycle.The investigation of optimization methods for an industrial centrifugal pump was carried out to reduce the intensity of pressure fluctuation to extend the lifecycle of these devices.Considering the time-consuming transient simulation of unsteady pressure,a novel optimization strategy was proposed by discretizing design variables and genetic algorithm.Four highly related design parameters were chosen,and 40 transient sample cases were generated and simulated using an automatic program.70%of them were used for training the surrogate model,and the others were for verifying the accuracy of the surrogate model.Furthermore,a modified discrete genetic algorithm(MDGA)was proposed to reduce the optimization cost owing to transient numerical simulation.For the benchmark test,the proposed MDGA showed a great advantage over the original genetic algorithm regarding searching speed and effectively dealt with the discrete variables by dramatically increasing the convergence rate.After optimization,the performance and stability of the inline pump were improved.The efficiency increased by more than 2.2%,and the pressure fluctuation intensity decreased by more than 20%under design condition.This research proposed an optimization method for reducing discrete transient characteristics in centrifugal pumps. | Wenjie Wang Qifan Deng Ji Pei Jinwei Chen Xingcheng Gan | 2023 | Chinese Journal of Mechanical Engineering2023，36，4： | 0 |
| 17 | Effects of medium fluid cavitation on fluctuation characteristics of magnetic fluid seal interface in agricultural centrifugal pump显示文摘The fluctuation law of magnetic fluid seal interface of an agricultural centrifugal pump is theoretically unknown;the pressure and velocity fluctuations are crucial factors that cause interface fluctuation.In this study,the pressure and velocity fluctuations of the sealing interface on an agricultural centrifugal pump during cavitation were investigated based on the methods of Ansys CFX numerical calculation and experimental verification.The results demonstrated that at the same flow rate,the pressure fluctuation amplitude of the sealing interface decreased gradually from the shaft surface to the bottom of the polar tooth.At different flow rates,the amplitude of the pressure fluctuation decreased with an increase in the flow rate.The cavitation of the medium aggravated the impact and water hammer of the liquid,leading to the occurrence of the jitter phenomenon in the sealing interface to accelerate the fluctuation frequency of the axial velocity of the sealing liquid,which accelerated the emulsification of the magnetic fluid.This law can provide a reference for the design of magnetic fluid sealing devices for agricultural centrifugal pumps. | Zhenggui Li Wangxu Li Qifan Wang Ru Xiang Jie Cheng Wei Han Zhaoqiang Yan | 2021 | International Journal of Agricultural and Biological Engineering2021，14，6： | 0 |
| 18 | USP14 regulates cell cycle progression through deubiquitinating CDK1 in breast cancer显示文摘Abnormal proliferation and cell cycle perturbation are the main hallmarks of breast cancer.Cyclin-dependent kinase 1(CDK1)is one of the key kinases for cell transition from the G2 phase to M phase during the cell cycle progression.However,little is known about the degradation mechanisms of CDK1.USP14(ubiquitin-specific processing protease 14)is an important proteasome-associated deubiquitinase that is critical for proteome homeostasis and plays a crucial role in the initiation and development of cancer.In this study,we find that USP14 shows high expression in breast cancer cells and results in the abnormal proliferation of cancer cells.Furthermore,we examine cell cycle distribution by flow cytometry and find that inhibition of USP14 causes cell cycle arrest in G2/M phase.As CDK1 is the key kinase in G2/M phase,we detect the interaction between USP14 and CDK1 and the effect of USP14 on the deubiquitination of CDK1.The results reveal that USP14 interacts with CDK1 and stabilizes CDK1 by deubiquitinating K48-linked ubiquitination.In conclusion,our findings reveal an indispensable role of USP14 in regulating cell cycle progression by stabilizing CDK1 in breast cancer,suggesting that USP14 may be used as a potential therapeutic target in breast cancer therapy. | Yuhan Liu Jing Xu Yanan Wang Mingxi Gan Qifan Hu Jianbin Wang Tianyu Han | 2022 | Acta Biochimica et Biophysica Sinica2022，54，11： | 0 |
| 19 | Crystal structure and functional implication of the RUN domain of human NESCA显示文摘NESCA,a newly discovered signaling adapter protein in the NGF-pathway,contains a RUN domain at its N-terminus.Here we report the crystal structure of the NESCA RUN domain determined at 2.0-Åresolution.The overall fold of the NESCA RUN domain comprises nine helices,resembling the RUN domain of RPIPx and the RUN1 domain of Rab6IP1.However,compared to the other RUN domains,the RUN domain of NESCA has significantly different surface electrostatic distributions at the putative GTPase-interacting interface.We demonstrate that the RUN domain of NESCA can bind H-Ras,a downstream signaling molecule of TrkA,with high affinity.Moreover,NESCA RUN can directly interact with TrkA.These results provide new insights into how NESCA participates in the NGF-TrkA signaling pathway. | Qifan Sun Chuanhui Han Lan Liu Yizhi Wang Hongyu Deng Lin Bai Tao Jiang | 2012 | Protein & Cell2012，3，8： | 0 |
| 20 | Carbon nanocages bridged with graphene enable fast kinetics for dual-carbon lithium-ion capacitors显示文摘Lithium-ion capacitors(LICs) combining the advantages of lithium-ion batteries and supercapacitors are considered a promising nextgeneration energy storage device. However, the sluggish kinetics of battery-type anode cannot match the capacitor-type cathode, restricting the development of LICs. Herein, hierarchical carbon framework(HCF) anode material composed of 0D carbon nanocage bridged with 2D graphene network are developed via a template-confined synthesis process. The HCF with nanocage structure reduces the Li^(+) transport path and benefits the rapid Li^(+) migration, while 2D graphene network can promote the electron interconnecting of carbon nanocages. In addition, the doped N atoms in HCF facilitate to the adsorption of ions and enhance the pseudo contribution, thus accelerate the kinetics of the anode. The HCF anode delivers high specific capacity, remarkable rate capability. The LIC pouch-cell based on HCF anode and active HCF(a-HCF) cathode can provide a high energy density of 162 Wh kg^(-1) and a superior power density of 15.8 kW kg^(-1), as well as a long cycling life exceeding 15,000cycles. This study demonstrates that the well-defined design of hierarchical carbon framework by incorporating 0D carbon nanocages and 2D graphene network is an effective strategy to promote LIC anode kinetics and hence boost the LIC electrochemical performance. | Shani Li Yanan Xu Wenhao Liu Xudong Zhang Yibo Ma Qifan Peng Xiong Zhang Xianzhong Sun Kai Wang Yanwei Ma | 2024 | Green Energy & Environment2024，9，3： | 0 |
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