|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Localization of phosphorylated TrkA in carrier vesicles involved in its nuclear translocation in U251 cell line显示文摘A number of transmembrane receptors are targeted to the nucleus and convincingly localized therein. However, what remains a conundrum is how these cell-surface receptors end up in the nucleus. In this study, we reported that the transmembrane receptor phosphorylated TrkA was located in a series of carrier vesicles, including ring-like vesicles near the plasma membrane, large core vesicles and small dense core vesicles around the nuclei, as well as in the nucleus in human glioma cell line U251 using immunocytochemistry and immunofluorescence staining. Meanwhile, we also showed that small dense core vesicles budded from large core vesicles, and interacted with the nuclear envelope. Accordingly, our results suggested that such a series of membrane compartments might be involved in the pathway of nuclear translocation of the transmembrane receptor TrkA. | GONG AiHua, ZHANG ZhiJian, XIAO DeSheng, YANG Yong, WANG YongZhong & CHEN YongChang School of Medicine, Jiangsu University, Zhenjiang 212013, China | 2007 | Science China(Life Sciences)2007，50，2： | 8 |
| 2 | Vesicular Li3V2(PO4)3/C hollow mesoporous microspheres as an efficient cathode material for lithium-ion batteries显示文摘Vesicular lithium vanadium phosphate/carbon hollow mesoporous microspheres were fabricated using a facile polyvinylpyrrolidone-assisted aerosol-spray-assisted method and subsequent heat-treatment. While changing the content of polyvinylpyrrolidone, we found that carbon content was adjustable on the surface of lithium vanadium phosphate. By optimizing the carbon content among the composites, the electrochemical performance can be enhanced significantly. The results of electrochemical performance tests suggested that the samples exhibited good cycle performance and high discharge capability in the voltages between 3.0-4.8 V. The observed excellent electrochemical performances could be attributed to the proper content of carbon coating and the vesicular hollow mesoporous microsphere structure, increasing the transmission rate of lithium ions and reducing the structural change during charging and discharging effectively. | Hongxia Sun Haoran Du Mengkang Yu Kuangfu Huang Nan Yu Baoyou Geng | 2019 | Nano Research2019，12，8： | 4 |
| 3 | Increased VGLUT3 involved in visceral hyperalgesia in a rat model of irritable bowel syndrome显示文摘AIM:To investigate the activity of vesicular glutamate transporter-3(VGLUT3) in a visceral hyperalgesia rat model of irritable bowel syndrome,and the role of mast cells(MCs).METHODS:Transient intestinal infection was inducedby oral administration of Trichinella spiralis larvae in rats.On the 100th day post-infection(PI),the rats were divided into an acute cold restraint stress(ACRS)group and a non-stressed group.Age-matched untreated rats served as controls.The abdominal withdrawal reflex was used to measure the visceromotor response to colorectal distension(CRD).The expression levels of VGLUT3 in peripheral and central neurons were analyzed by immunofluorescence and western blotting.RESULTS:VGLUT3 expression in the L6S1 dorsal root ganglion cells was significantly higher in the PI group than in the control group(0.32±0.009 vs0.22±0.008,P<0.01),and there was no significant difference in the expression of VGLUT3 between MCdeficient rats and their normal wild-type littermates.Immunofluorescence showed that the expression levels of VGLUT3 in PI+ACRS rats were enhanced in the prefrontal cortex of the brain compared with the control group.CONCLUSION:VGLUT3 is involved in the pathogenesis of visceral hyperalgesia.Coexpression of c-fos,5-hydroxytryptamine and VGLUT3 after CRD was observed in associated neuronal pathways.Increased VGLUT3 induced by transient intestinal infection was found in peripheral nerves,and was independent of MCs.Moreover,the expression of VGLUT3 was enhanced in the prefrontal cortex in rats with induced infection and stress. | Chang-Qing Yang Li-Ping Duan Pei-Tang Qiao Li Zhao Li-Li Guo | 2015 | World Journal of Gastroenterology2015，21，10： | 4 |
| 4 | Thymosin Alpha-1 Inhibits Complete Freund’s Adjuvant-Induced Pain and Production of Microglia-Mediated Pro-inflammatory Cytokines in Spinal Cord显示文摘Activation of inflammatory responses regulates the transmission of pain pathways through an integrated network in the peripheral and central nervous systems.The immunopotentiator thymosin alpha-1(Tal)has recently been reported to have anti-inflammatory and neuroprotective functions in rodents.However,how Tα1 affects inflammatory pain remains unclear.In the present study,intraperitoneal injection of Tal attenuated complete Freund's adjuvant(CFA)-induced pain hypersensitivity,and decreased the up-regulation of pro-inflammatory cytokines(TNF-α,IL-1β,and IL-6)in inflamed skin and the spinal cord.We found that CFA-induced peripheral inflammation evoked strong microglial activation,but the effect was reversed by Tα1.Notably,Tα1 reversed the CFA-induced up-regulation of vesicular glutamate transporter(VGLUT)and down-regulated the vesicular γ-aminobutyric acid transporter(VGAT)in the spinal cord.Taken together,these results suggest that Tα1 plays a therapeutic role in inflammatory pain and in the modulation of microgliainduced pro-inflammatory cytokine production in addition to mediation of VGLUT and VGAT expression in the spinal cord. | Yunlong Xu Yanjun Jiang Lin Wang Jiahua Huang Junmao Wen Hang Lv Xiaoli Wu Chaofan Wan Chuanxin Yu Wenjie Zhang Jiaying Zhao Yinqi Zhou Yongjun Chen | 2019 | Neuroscience Bulletin2019，35，4： | 4 |
| 5 | A Vesicular Stomatitis Virus-Based Vaccine Carrying Zika Virus Capsid Protein Protects Mice from Viral Infection显示文摘Dear Editor,Zika virus (ZIKV) is a mosquito-borne virus that belongs to the Flavivirus family along with dengue virus (DENV),yellow fever virus, West Nile virus, and Japanese encephalitis virus (Ming et al. 2016). ZIKV is a singlestranded positive-sense RNA virus encoding three structural proteins, including nucleocapsid protein C, prM/M,envelope glycoprotein E, and seven non-structural proteins.Since 2015. | Xiaodan Shi Jingping Hu Jing Guo Chuanjian Wu Sidong Xiong Chunsheng Dong | 2019 | Virologica Sinica2019，34，1： | 2 |
| 6 | Novel Vesicular Aqueous Two-Phase Systems显示文摘The interesting phenopmena of two aqueous phases coexisting in dilute aqueous solutions of sodium undecenoate-dodecyltrimethylammo brodride ndxture and sodium laurate-dodecyltrimethylammonium brondde mixture were investigated. Vesicles existing in both phases were shown by TEM images. The vesicles are dispersed in lower phase and flocculated in upper phase. Multilamellar structure of vesicles was found in the upper phase of sodium laurate-dodecyltrimethylammonium bromide system. | Guo Xi ZHAO Wang Lin YU Yu Jun GONG Bu Yao ZHU (Institute of Physical Chemistry, Peking University, Beijing 100871) | 1998 | Chinese Chemical Letters1998，9，11： | 2 |
| 7 | Vesicular nanocarrier based treatment of skin fungal infections: Potential and emerging trends in nanoscale pharmacotherapy显示文摘Occurrence of skin fungal infections is increasing nowadays and their presence is more prominent in patients suffering from immunocompromised diseases like AIDS. Skin fungal infections are a major cause of visits by patients to dermatology clinics. Although, a large number of antifungal agents are available for treatment of skin fungal infections, but, their toxic profile and physicochemical characteristics reduce therapeutic outcome. When these antifungal agents are delivered topically using conventional formulations like creams and gels, they may cause various side effects like redness, burning, and swelling at the site of application. Therefore, various vesicular formulations(phospholipid based or non phospholipid based) have been explored by pharmaceutical scientists to treat skin fungal infections topically. Vesicular formulation explored for the purpose are liposomes, ethosomes, transfersomes, transethosomes, niosomes, spanlastics, oleic acid vesicles, and nanoparticles.These formulations show various advantages like bioavailability enhancement of bioactives,high skin permeation power, no side effects at application site, dosing frequency reduction,and sustained drug release. Therefore, in the present article, we have discussed about the utility of various vesicular nanocarrier systems to treat skin fungal infections. | Shivani Verma Puneet Utreja | 2019 | Asian Journal of Pharmaceutical Sciences2019，14，2： | 1 |
| 8 | 一起牛水泡性口炎的治疗效果观察显示文摘水泡性口炎(vesicular stomatitis,VS)又名鼻疮(sore nose)、口疮(sore mouth)、伪口疮(pseudoaphthosis),'烂舌症'、'牛及马的口溃疡'等。本病是牛、马和猪的一种病毒性疾病。其特征为口腔黏膜、乳头皮肤及蹄冠部皮肤出现水泡及糜烂,但很少发生死亡。在临床上注意与口蹄疫的鉴别诊断。笔者用痘疱金刚配合高效救命针注射的方法进行治疗。 | 李世双 | 2014 | 青海畜牧兽医杂志2014，44，5： | 1 |
| 9 | 密切关注A型塞内卡病毒的流行与传播显示文摘引起新生仔猪死亡的原因有很多,既有传染病因素也有非传染病因素。常见的传染病因素主要是病毒和细菌感染,如猪繁殖与呼吸综合征病毒、猪流行性腹泻病毒、猪传染性胃肠炎病毒、轮状病毒和大肠杆菌等。 | 翁善钢 | 2016 | 养猪2016，，1： | 1 |
| 10 | Bidirectional regulation of Ca^(2+) in exo–endocytosis coupling显示文摘Vesicular exocytosis is a key process involved in neurotransmitter release,whereas vesicle recycling is critical for the homeostasis of plasma membrane structures and the maintenance of neurotransmission.Ca^2+ regulates several fundamental cellular processes(Zhu et al.,2016,Xiong and Zhu,2016).Especially,Ca^2+ influx plays a dual role of triggering vesicular exocytosis and modulating compensatory endocytosis in neurons and endocrine cells. | Qian Song Mingzhu Huang Bianbian Wang Xinjiang Kang Changhe Wang | 2018 | Science China(Life Sciences)2018，61，12： | 1 |
| 11 | Adolescent stress increases depression-like behaviors and alters the excitatory-inhibitory balance in aged mice显示文摘Background:Depression affects approximately 5% of elderly people and its etiology might be related to chronic stress exposure during neurodevelopmental periods.In this study,we examined the effects of adolescent chronic social stress in aged mice on depressive behaviors and the excitatory-inhibitory (E/I) balance in stress-sensitive regions of the brain.Methods:Sixty-four adolescent,male C57BL/6 mice were randomly assigned to either the 7-week (from post-natal days 29 to 77) social instability stress (stress group,n =32) or normal housing conditions (control group,n =32).At 15 months of age,16 mice were randomly selected from each group for a series of behavioral tests,including two depression-related tasks (the sucrose preference test and the tail suspension test).Three days following the last behavioral test,eight mice were randomly selected from each group for immunohistochemical analyses to measure the cell density of parvalbumin (PV+)-and calretinin (CR+)-positive gamma-aminobutyric-acid (GABA)ergic inhibitory inter-neurons,and the expression levels of vesicular transporters of glutamate-1 (VGIuT1) and vesicular GABA transporter (VGAT) in three stress-sensitive regions of the brain (the medial pre-frontal cortex [mPFC],hippocampus,and amygdala).Results:Behaviorally,compared with the control group,adolescent chronic stress increased depression-like behaviors as shown in decreased sucrose preference (54.96 ± 1.97% vs.43.11 ± 2.85%,t(22)=3.417,P =0.003) and reduced latency to immobility in the tail suspension test (92.77 ± 25.08 s vs.33.14 ± 5.95 s,t(25)=2.394,P =0.025),but did not affect anxiety-like behaviors and pre-pulse inhibition.At the neurobiologic level,adolescent stress down-regulated PV+,not CR+,inter-neuron density in the mPFC (F(1,39)=19.30,P < 0.001),and hippocampus (F(1,42)=5.823,P =0.020) and altered the CR+,not PV+,inter-neuron density in the amygdala (F(1,28)=23.16,P < 0.001).The VGluT1/VGAT ratio was decreased in all three regions (all F > 10.09,all P < 0.004),which suggests stress-induced hypoexcitability in these regions.Conclusions:Chronic stress during adolescence increased depression-like behaviors in aged mice,which may be associated with the F/I imbalance in stress-sensitive brain regions. | Hong-Li Wang Ya-Xin Sun Xiao Liu Han Wang Yu-Nu Ma Yun-Ai Su Ji-Tao Li Tian-Mei Si | 2019 | Chinese Medical Journal2019，，14： | 0 |
| 12 | PHOSPHOLIPID STUDY IN THE EARLY DEVELOPMENT OF GOAT EMBRYOS显示文摘The experiment is to study the distribution and content of phospholipid and its relationship with cellular differentiation in the goat preimplantation development by electron cytochemistry. The zygotes and embryos at 2-cell, 4-cell, 8-cell, 16-cell morula and early blastocyst stage collected from the superovulated Heilongjiang goats with FSH were used to do ultrastructural localization of phospholipid with the method reported by Dermer (J. | 杨增明 谭景和 秦鹏春 | 1990 | Chinese Science Bulletin1990，35，18： | 0 |
| 13 | Semireplication-competent vesicular stomatitis virus as a novel platform for oncolytic virotherapy显示文摘Among oncolytic viruses, the vesicular stomatitis virus(VSV) is especially potent and a highly promising agent for the treatment of cancer. But, even though effective against multiple tumor entities in preclinical animal models, replicationcompetent VSV exhibits inherent neurovirulence, which has so far hindered clinical development. To overcome this limitation,replication-defective VSV vectors for cancer gene therapy have been tested and proven to be safe. However, gene delivery was inefficient and only minor antitumor efficacy was observed. Here, we present semireplicationcompetent vector systems for VSV(sr VSV), composed of two trans-complementing, propagation-deficient VSV vectors. The de novo generated deletion mutants of the two VSV polymerase proteins P(phosphoprotein) and L(large catalytic subunit), VSVΔP and VSVΔL respectively, were used mutually or in combination with VSVΔG vectors. These sr VSV systems copropagated in vitro and in vivo without recombinatory reversion to replication-competent virus. The sr VSV systems were highly lytic for human glioblastoma cell lines,spheroids, and subcutaneous xenografts. Especially the combination of VSVΔG/VSVΔL vectors was as potent as wild-type VSV(VSV-WT) in vitro and induced long-term tumor regression in vivo without any associated adverse effects. In contrast, 90% of VSV-WT-treated animals succumbed to neurological disease shortly after tumor clearance. Most importantly, even when injected into the brain, VSVΔG/VSVΔL did not show any neurotoxicity. In conclusion, sr VSVis a promising platform for virotherapeutic approaches and also for VSV-based vector vaccines, combining improved safety with an increased coding capacity for therapeutic transgenes, potentially allowing for multipronged approaches. | Alexander Muik Catherine Dold Yvonne Gei Andreas Volk Marina Werbizki Ursula Dietrich Dorothee von Laer | 2015 | 世界最新医学信息文摘2015，15，5： | 0 |
| 14 | Effect of up-regulated expression of tumor suppressor gene p14^(ARF) on apoptosis of chronic myeloid leukemia cells显示文摘Objective To investigate the effect of up-regulated expression of tumor suppressor gene p14ARFon apoptosis of chronic myeloid leukemia (CML) cells and its interaction with imatinib.Methods Tumor suppressor gene p14ARFwas transduced into K562 (K562-p14ARF) and 4blast crisis primary CML cells (CML-BC 1-4) using vesicular stomatitis virus glycoprotein (VSV-G) | 白元松 | 2013 | China Medical Abstracts(Internal Medicine)2013，30，3： | 0 |
| 15 | Transverse myelitis after infection with varicella zoster virus in patient with normal immunity: A case report显示文摘BACKGROUND Varicella zoster virus(VZV)is a human neurotropic and double-stranded DNA alpha-herpes virus.Primary infection with VZV usually occurs during childhood,manifesting as chickenpox.Reactivation of latent VZV can lead to various neurological complications,including transverse myelitis(TM);although cases of the latter are very rare,particularly in newly active VZV infection.CASE SUMMARY We report here an unusual case of TM in a middle-aged adult immunocompetent patient that developed concomitant to an active VZV infection.The 46-year-old male presented with painful vesicular eruption on his left chest that had steadily progressed to involvement of his back over a 3-d period.Cerebrospinal fluid testing was denied,but findings from magnetic resonance imaging and collective symptomology indicated TM.He was administered antiviral drugs and corticosteroids immediately but his symptom improvement waxed and waned,necessitating multiple hospital admissions.After about a month of repeated treatments,he was deemed sufficiently improved for hospital discharge to home.CONCLUSION VZV myelitis should be suspected when a patient visits the outpatient pain clinic with herpes zoster showing neurological symptoms. | Daehun Yun Soo Young Cho Wan Ju Eun Hyoung Seo | 2021 | World Journal of Clinical Cases2021，9，33： | 0 |
| 16 | A model of necrotic degeneration in Drosophila显示文摘Cell death during development or pathological conditions can be classified into three types based on morphology and genetic pathways: apoptosis, autophagic necrosis and | Lei Liu School of Life Sciences, Peking University | 2009 | 生物物理学报2009，25，S1： | 0 |
| 17 | Endocytosis and Vesicular Transport of Plasmid DNA in Cells During Electric Field-Mediated Gene Delivery显示文摘Pulsed electric field has been used widely as a nonviral approach to improving gene delivery in basic and translational research[1-2].The technique has been called electrotransfection(ET),electroporation,electrogene transfer,and gene electroinjection in the literature [1,3].It has a great potential to improve clinical treatment of diseases through delivery of vaccines and therapeutic genes,genome and epigenome editing,and generation of human induced pluripotent stem cells for tissue engineering[1-3].During ET,extracellular transport of plasmid DNA(pDNA)relies on electrophoresis,which is critical for applications in vivo.However,mechanisms of intracellular transport remain to be understood.The lack of understanding has hindered the translation of ET technology to the clinic.It is well known that pulsed electric field can generate transient hydrophilic pores in the plasma membrane(i.e.,electroporation)that permit membrane-impermeant molecules to enter cells.Although the pores have yet to be visualized directly under a microscope,the electric field-induced membrane permeabilization has been demonstrated through experimental measurements of electrical conductance of synthetic lipid membranes and plasma membranes,direct observation of fluorescent markers crossing the membranes facing both cathode and anode,and numerical simulations of the membrane permeabilization[1,3].Results from the simulations have predicted that the cutoff size of the pores is on the order of a few hundred nanometers,and the lifetime of the pores that are larger than 100 nm is on the order of 10 msec.Although these data provide a solid evidence of the membrane permeabilization,recent studies have demonstrated that the generation of the pores is insufficient for ET[1,4].The reasons are as follows.First,the lifetime of the pores is several orders of magnitude shorter than the time scale for pDNA uptake,which is on the order of 10 min.Second,complex formation between pDNA and plasma membrane is a necessary condition for successful gene transfer.Third,inhibition of clathrin mediated endocytosis or Rac-1 dependent micropinocytosis can reduce the amount of pDNA internalized by cells [1].Finally,we demonstrate that few pDNA molecules can be observed in the cytosol that are not associated with the intracellular vesicles[5],suggesting that pDNA uptake is mediated by endocytosis.In addition to the internalization,ET requires the pDNA in the cytoplasm to reach the nucleus.To understand mechanisms of intracellular trafficking of pDNA,we have examined time-dependent pDNA distributions in cells,quantitatively determined percentages of pDNA molecules associated with different endocytic compartments using transmission electron microscopy(TEM),and investigated different approaches to facilitate cytoplasmic transport and nuclear entry of pDNA.Our data have shown that electrotransfected pDNA is located in different vesicular ultrastructures at or near the plasma membrane at10 min post application of electric pulses[5].In the hard-to-transfect cells(e.g.,4T1),pDNA penetration from the cell surface is less active,and the total number of vesicular structures associated with pDNA is low,compared to those in the easyto-transfect cells(e.g.,COS7).Our data have also shown that macropinocytosis is the most common pathway shared by all types of cells.To investigate how improve pDNA transport in cells,we have photochemically treated cells to non-specifically induce pDNA escape from intracellular vesicles,or blocked endosome and autophagic vacuole maturation through treatment of cells with Bafilomycin Al,an inhibitor of vacuolar H+ATPase.Our data demonstrate that both treatments can lead to reduction of ET efficiency although the treatment for inducing endosomal escape can enhance poly-L-lysine mediated gene delivery.These data suggest that the vesicles play an important role in protecting the naked pDNA during intracellular trafficking.The nuclear envelope is another major barrier to ET.To facilitate the nuclear entry,we have examined three different approaches.One is to synchronize the nuclear envelope breakdown(NEBD)prior to ET;the second approach is to pre-treat cells with a nuclear pore dilating agent(i.e.,trans-1,2-cyclohexanediol);and the third one is to incorporate a nuclear targeting sequence(NTS)(i.e.,SV40)into the pDNA.Our data have shown that the synchronization of the NEBD can significantly improve the ET efficiency without compromising the cell viability.The nuclear pore dilation can improve the ET as well but the dilating agent is cytotoxic.The incorporation of NTS into pDNA can improve the gene delivery efficiency but the improvement is cell-type dependent,suggesting that the NTS has to be screened and optimized for the cells of interest.In summary,the transient pores in the plasma membrane induced by the electric pulses will enable cellular uptake of membrane-impermeant molecules up to the size of small proteins.Larger molecules(e.g.,pDNA)have to be internalized via endocytic processes triggered by the pulsed electric field.Within the cells,pDNA transport is mediated by vesicles and can be blocked by non-specific escape from vesicles or inhibition of vesicle maturation.The nuclear entry of pDNA can be enhanced,without compromising cell viability,through the use of the NTS or the synchronization of the NEBD. | Fan Yuan | 2019 | 医用生物力学2019，34，A01： | 0 |
| 18 | 如何鉴定谷氨酸能神经元显示文摘答:谷氨酸能神经元是中枢神经系统内最为重要的兴奋性神经元。谷氨酸作为神经递质,与其它类型的神经递质不同。谷氨酸在所有细胞中都存在,也就是说含有谷氨酸的神经元不一定都是谷氨酸能的。只有当神经元中谷氨酸被囊泡谷氨酸转运体(vesicular glutamate transporters,VGLUTs)转运进入突触囊泡(synaptic vesicle),突触囊泡再与突触前膜融合后,谷氨酸才能被释放到突触间隙中并作为神经递质发挥作用,才可以确定该神经元是谷氨酸能的。 | 周瑞 | 2017 | 中国比较医学杂志2017，27，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员