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| 1 | Assessment of the immune responses to Treponema pallidum Gpd DNA vaccine adjuvanted with IL-2 and chitosan nanoparticles before and after Treponema pallidum challenge in rabbits显示文摘Syphilis is a multistage,sexually transmitted disease caused by the spirochete,Treponema pallidum(Tp).A significantly high incidence of syphilis has been reported in several countries,including China,and there is an urgent need for the development of efficacious vaccines against syphilis.DNA vaccines are a major breakthrough in the field of vaccination with several advantages over traditional vaccines.Animal model studies of Tp DNA vaccines have not been reported elsewhere but our previous reports describe the development of a single-gene Tp DNA vaccine and preclinical immunization study.In this study,chitosan(CS) nanoparticles were used as a vector and an interleukin-2 expression plasmid(pIL-2) as an adjuvant to enhance a TpGpd DNA vaccine candidate(pTpGpd) in a rabbit Tp skin challenge model.At week 8 after the first immunization,three rabbits from each group were used to determine cytokine measurements and spleen lymphocyte proliferation assay.pTpGpd in combination with pIL-2 wrapped with CS led to the greatest enhancement of anti-TpGpd antibodies and T-cell proliferation.During infection,levels of anti-TpGpd antibodies and T-cell proliferation were measured.Both the serum special IgG and IL-2,interferon-γ were significantly increased by the co-injection of the IL-2 plasmid compared with the injection of TpGpd DNA alone(P<0.05).Furthermore,IL-2 plasmid coinjection efficiently enhanced the antigen-specific lymphocyte proliferation response.Additionally,the ratios of positive skin lesions and ulcer lesions in groups immunized with pTpGpd were significantly lower than those of the pIL-2,CS or pIL-2 mixed with CS control groups(P<0.001).CS vectored and pIL-2 adjuvanted pTpGpd immunized animals exhibited the lowest rates of positive skin tests(8.33%) and ulcer lesions(4.17%) and the fastest recovery(42 d).These experiments indicate that co-injection of a pIL-2 plasmid with pTpGpd DNA vaccine wrapped with CS can significantly strengthen the long-term stability of immune response during infection,efficiently improve the protective effect against T.pallidum spirochetes infection and attenuate syphilitic lesion development. | ZHAO FeiJun ZHANG XiaoHong LIU ShuangQuan ZENG TieBing YU Jian GU WeiMing ZHANG YueJun CHEN Xi WU YiMou | 2013 | Science China(Life Sciences)2013，56，2： | 8 |
| 2 | Association between dietary habits and recurrent respiratory infection in children: A case-control study显示文摘Objective:To explore the association between dietary habits and recurrent respiratory infection(RRI)in children aged 0e14 years.Methods:This caseecontrol study compared dietary data of children with(cases)and without RRI(controls)collected via structured questionnaire.Participants were recruited from Chinese medicine clinics,hospitals,and children’s learning institutions in Beijing.A logistic regression analysis and odds ratio(OR)calculations were conducted using SPSS 17.0 software.Results:A total of 241 questionnaires were collected(case:control ratio:approximately 2:1).Frequent consumption of processed foods(OR Z 2.988,95%confidence intervals 1.375e6.491)and high-sugar foods(OR Z 2.268,95%confidence intervals 1.163e4.424),frequent picky eating(OR Z 2.614,95%confidence intervals 1.363e5.014),and a meat-heavy diet with fewer vegetables(OR Z 1.830,95%confidence intervals 1.358e2.467)correlated positively correlated with RRI.Additionally,57.80%of the children with RRI were addicted to high-sugar foods,compared with 41.57%of the children without RRI(P Z.015).Furthermore,63.16%of the children with RRI were picky eaters,compared with 48.31%of the children without RRI(P Z.024).Finally,30.92%of the children with RRI frequently consumed processed foods,compared with only 17.98%of the children without RRI(P Z.027Conclusion:Although RRI correlates positively with several dietary habits,in the future,prospective cohort studies with larger samples are needed to generalize these findings. | Wang Zhang He Yu Jinbang Shang Tiegang Liu Jiaju Ma Xiaohong Gu | 2015 | Journal of Traditional Chinese Medical Sciences2015，2，2： | 5 |
| 3 | Phosphine production in anaerobic wastewater treatment under tetracycline antibiotic pressure显示文摘The influence of tetracycline(TC) antibiotics on phosphine(PH_3) production in the anaerobic wastewater treatment was studied. A lab-scale anaerobic baffled reactor with three compartments was employed to simulate this process. The reactor was operated in a TC-absence wastewater and 250 μg/L TC-presence wastewater for three months after a start-up period,respectively. The responses of p H, oxidation–reduction potential(ORP), chemical oxygen demand(COD), total phosphorus(TP), enzymes activity(dehydrogenase and acid phosphatase),and microbial community were investigated to reveal the effect of TC on PH_3 production.Results suggested that the dehydrogenase(DH) activity, acid phosphatase(ACP) activity and COD have positive relationship with PH_3 production, while p H, ORP level and the TP in liquid phase have negative relationship with PH_3 production. With prolonged TC exposure, decrease in p H and increase in DH activity are beneficial to PH_3 production, while decrease in COD and ACP activity are not the limiting factors for PH_3 production. | Meiqing Lu Xiaojun Niu Weiyi Chen Zhu Liang Sheng You Xiaohong Gu | 2018 | Journal of Environmental Sciences2018，30，7： | 5 |
| 4 | Dual effects of α-synuclein on neurotoxicity induced by low dosage of rotenone are dependent on exposure time in dopaminergic neuroblastoma cells显示文摘This study was designed to investigate the effects of α-synuclein on toxicity induced by long-term exposure to relatively low concentrations of rotenone.Compared with the control groups,the inhibition of cell viability which overexpressed α-synuclein(SH-SY5Y-Syn) improved after 1 and 2 weeks of rotenone treatment.The complex I activity was greater and the mitochondrial membrane swelling intensity was reduced after 1 and 2 weeks of treatment,which indicated that α-synuclein,at least in part,resists the rotenone-induced oxidative stress.The results indicate that α-synuclein has a dual effect on toxicity of rotenone according to exposure time in human SH-SY5Y cells. | LU LingLing,GU Li,LIANG Yuan,SUN XiaoHong,DUAN ChunLi & YANG Hui Beijing Institute for Neuroscience,Capital Medical University,The Beijing Center of Neural Regeneration and Repairingr,Key Laboratory for Neurodegenerative Disease of the Ministry of Education,Beijing 100069,China | 2010 | Science China(Life Sciences)2010，53，5： | 3 |
| 5 | The minimization of non-equilibrium plasma source at high pressure显示文摘The density of plasma produced by atmospheric non-equilibrium plasma source is the function of en-ergy dissipation rate in ionization electric field and gas particles momentum.The experiment shows that the plasma density highly rises with the increasing of energy dissipation rate and gas particles momentum.When the energy dissipation rate of activation field is 2.18 Wh/m3 and the average gas particles momentum is 109×10-22 g·m/s,the air throughput of plasma source whose volume is only 2.5 cm3 can be up to 12 m3/h and the density of plasma can be up to 1010 cm-3.The research can develop a method of producing minitype plasma source which is low energy consumption but high ion concen-tration used for chemical industry,environmental engineering and military. | BAI MinDi QIU XiuMei LIU Dong YANG Bo ZHOU JianGang XUE XiaoHong GU JianLong | 2008 | Chinese Science Bulletin2008，53，21： | 3 |
| 6 | The efficacy of NP11-4-derived immunotoxin scFv-artesunate in reducing hepatic fibrosis inducedby Schistosoma japonicum in mice显示文摘Schistosomiasis is one of the most prevalent parasitic diseases in China,and hepatic fibrosis caused by schistosome infection is the principal cause of death.The aim of this study was to evaluate the efficacy of NP11-4derived immunotoxin scFv-artesunate on Schistosoma japonicum-induced hepatic fibrosis.A single-chain variable fragment (scFv) was generated from the murine anti-Schistosoma japonicum (S.japanicum) monoclonal antibody NP11-4.The scFv was expressed as a soluble protein and purified by Ni-affinity chromatography.After conjugation with artesunate,the binding ability with soluble egg antigens (SEA) was determined by an enzyme-linked immunosorbent assay (ELISA).The biological activity of purified scFv,scFv-artesunate (immunotoxin),and artesunate was detected in vivo.Image-Pro Plus software was used to analyze the size of egg granuloma and the extent of liver fibrosis.The recombinant scFv expession vector was constructed and expressed successfully.After purification by a His-trap Ni-affinity column,the scFv yield was approximately 0.8 mg/L of culture medium.ELISA results showed that chemical conjugation did not affect the binding activity of the immunotoxin.Our animal experiments indicated that the immunotoxin could significantly reduce the size of egg granuloma in the liver and inhibit hepatic fibrosis.The immunotoxin could be used as a promising candidate in the targeted therapy of S.japonicum-induced hepatic fibrosis. | Hong Li Chunyan Gu Yongya Ren Yang Dai Xiaojuan Zhu Jing Xu Yuhua Li Zhenning Qiu Jin Zhu Yinchang Zhu Xiaohong Guan Zhenqing Feng | 2011 | The Journal of Biomedical Research2011，25，2： | 3 |
| 7 | Drug resistance mutation profiles of the drug-nave and first-line regimen-treated HIV-1-infected population of Suzhou, China显示文摘Little is known about the prevalence of drug-resistant mutations in HIV-1-positive individuals in Suzhou, China. To elucidate the transmitted drug resistance(TDR) and acquired drug resistance mutation(ADR) profiles, we collected blood specimens from 127 drug-naive and 117 first-line drugtreated HIV-1-infected individuals sampled from 2014 to 2016 in Suzhou. We successfully amplified po/fragments from 100 drug-naive and 20 drug-treated samples. We then determined the drugresistant mutations to protease(PR) and reverse-transcriptase(RT) inhibitors according to the Stanford drug resistance database. Overall, 11 and 13 individuals had transmitted(drug-naive group) and acquired(treated group) resistance mutations, respectively. Six transmitted drugresistant mutations were found, including two mutations(L33F and L76V) in the protease region and four(K70N/E and V179D/E) in the RT region. Only L76 V was a major mutation, and K70N/E and V179D/E are known to cause low-level resistance to RT inhibitors. All 13 treated participants who had major drug resistance mutations demonstrated intermediate to high resistance to efavirenz and nevirapine, and six had a treatment duration of less than three months. No major mutations to RT inhibitors were found, implying that the epidemic of transmitted resistance mutations was not significant in this area. Our results suggest that more frequent virus load and drug resistance mutation tests should be conducted for individuals receiving antiretroviral treatment, especially for newly treated patients. Our research provides insights into the occurrence of HIV-1 drug resistance in Suzhou and will help to optimize the treatment strategy for this population. | Tingting Li Feng Qian Ting Yuan Weilu Xu Li Zhu Jinlong Huang Haiyan Wang Yueping Zhu Yinling Wang Xiaohong Li Saihong Gu Zhuqing Tan Hui Chen Xiangrong Luo Wei Zhu Weniuan Lu Ping Xu Ming Li Yuying Chen Yong Gao Rongge Yang Chuanwu Zhu Binlian Sun | 2017 | Virologica Sinica2017，32，4： | 2 |
| 8 | New insights for infection mechanism and potential targets of COVID-19:Three Chinese patent medicines and three Chinese medicine formulas as promising therapeutic approaches显示文摘The coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)with high pathogenicity and infectiousness has become a sudden and lethal pandemic worldwide.Currently,there is no accepted specific drug for COVID-19 treatment.Therefore,it is extremely urgent to clarify the pathogenic mechanism and develop effective therapies for patients with COVID-19.According to several reliable reports from China,traditional Chinese medicine(TCM),especially for three Chinese patent medicines and three Chinese medicine formulas,has been demonstrated to effectively alleviate the symptoms of COVID-19 either used alone or in combination with Western medicines.In this review,we systematically summarized and analyzed the pathogenesis of COVID-19,the detailed clinical practice,active ingredients investigation,network pharmacology prediction and underlying mechanism verification of three Chinese patent medicines and three Chinese medicine formulas in the COVID-19 combat.Additionally,we summarized some promising and high-frequency drugs of these prescriptions and discussed their regulatory mechanism,which provides guidance for the development of new drugs against COVID-19.Collectively,by addressing critical challenges,for example,unclear targets and complicated active ingredients of these medicines and formulas,we believe that TCM will represent promising and efficient strategies for curing COVID-19 and related pandemics. | Kexin Jia Yijie Li Tiegang Liu Xiaohong Gu Xiaojiaoyang Li | 2023 | Chinese Herbal Medicines2023，15，2： | 2 |
| 9 | Growth and feeding habits of invasive Pseudorasbora parva in the Chabalang Wetland(Lhasa,China) and its trophic impacts on native fish显示文摘In recent years, fish invasion has become one of the main reasons for the decline of native fish stocks. Pseudorasbora parva is considered one of the major invasive species worldwide. The present study investigated the fish resources of the Chabalang Wetland (Lhasa, Tibet) during diff erent seasons in 2009 and 2013. Four hundred and twelve individuals were subsampled to estimate age, growth, and feeding habit of P . parva . Furthermore, food relationships between P . parva and the native Schizothoracinae fish were also examined. The results revealed a significant shift in species composition and community structure characterized by the disappearance of native fish and outbreak of non-native fish. The percentage of nonnative P . parva in the fish collections significantly increased from 33.64% in 2009 to 64.08% in 2013. The standard length (SL) ranged from 22.00 to 78.71 mm, and their age was 1–5 a. The von Bertalanff y function was used to model the observed length-at-age data as L t =112.19(1–e^-0.1495 ( t +0.8012)) for females and as L t =123.12 (1–e^-0.1500 ( t +0.7132)) for males. The results indicated that P . parva in Tibet has lower growth and mortality rates compared with that from the native ranges. Ninety-seven prey taxa belonging to 9 prey categories were identified in the gut of 38 P . parva . P . parva can be considered a generalized and opportunistic predator, competing with the native fish, especially Schizothorax o’connori , Schizopygopsis younghusbandi younghusbandi , and Ptychobarbus dipogon , for Bacillariophyta and Chironomid larvae. This is an important reason for the decline in native fish population. | DING Huiping GU Xiaohong ZHANG Zhiming HUO Bin LI Dapeng XIE Congxin | 2019 | Journal of Oceanology and Limnology2019，37，2： | 2 |
| 10 | Optimization of ultrasonic/microwave assisted extraction (UMAE) of polysaccharides from Inonotus obliquus and evaluation of its anti-tumor activities显示文摘 | Yiyong Chen Xiaohong Gu Sheng-quan Huang Jinwei Li Xin Wang Jian Tang | 2010 | International Journal of Biological Macromolecules2010，，4： | 2 |
| 11 | Effects of components in culture medium on glutamate decarboxylase activity and γ-aminobutyric acid accumulation in foxtail millet ( Setaria italica L.) during germination显示文摘 | Qingyun Bai Meiqing Chai Zhenxin Gu Xiaohong Cao Yan Li Kunlun Liu | 2009 | Food Chemistry2009，，1： | 2 |
| 12 | Assessment of pollutant loads discharged from aquaculture ponds around Taihu Lake, China显示文摘 | Chunfang Cai Xiaohong Gu Yuantu Ye Caigeng Yang Xiuying Dai Dongxing Chen Chao Yang | 2012 | Aquac Res2012，，5： | 1 |
| 13 | Influence of the extent ofenzymatic hydrolysis on the functional properties of protein hydrolysatefrom grass carp(Ctenopharyngodon idella)skin显示文摘 | WASSWA J TANG Jian GU Xiaohong | 2007 | Food Chemistry2007，104，4： | 1 |
| 14 | Optimization of ultrasonic/microwave assisted extraction (UMAE) of polysaccharides from Inonotus obliquus and evaluation of its anti-tumor activities显示文摘 | CHEN Yiyong GU Xiaohong HUANG Shengquan | 2010 | International Journal of Biological Macromoleeules2010，46，4： | 1 |
| 15 | Optimization assisted extraction (UMAE) of polysaccharides from Inonotus obliquus and evaluation of its anti-tumor activities显示文摘 | CHEN Yiyong GU Xiaohong HUANG Shengquan ct al | 2010 | International Journal of Biological Macromolecules2010，46，4： | 1 |
| 16 | CTCs Detection and Whole-exome Sequencing Might Be Used to Differentiate Benign and Malignant Pulmonary Nodules显示文摘Background and objective Low-density computed tomography(LDCT)improved early lung cancer diagnosis but introduces an excess of false-positive pulmonary nodules data.Hence,accurate diagnosis of early-stage lung cancer remains challenging.The purpose of the study was to assess the feasibility of using circulating tumour cells(CTCs)to differentiate malignant from benign pulmonary nodules.Materials and methods 122 patients with suspected malignant pulmonary nodules detected on chest CT in preparation for surgery were prospectively recruited.Peripheral blood samples were collected before surgery,and CTCs were identified upon isolation by size of epithelial tumour cells and morphological analysis.Laser capture microdissection,MALBAC amplification,and whole-exome sequencing were performed on 8 samples.The diagnostic efficacy of CTCs counting,and the genomic variation profile of benign and malignant CTCs samples were analysed.Results Using 2.5 cells/5 m L as the cut-off value,the area under the receiver operating characteristic curve was of 0.651(95%confidence interval:0.538-0.764),with a sensitivity and specificity of 0.526 and 0.800,respectively,and positive and negative predictive values of 91.1%and 30.3%,respectively.Distinct sequence variations differences in DNA damage repair-related and driver genes were observed in benign and malignant samples.TP53 mutations were identified in CTCs of four malignant cases;in particular,g.7578115T>C,g.7578645C>T,and g.7579472G>C were exclusively detected in all four malignant samples.Conclusion CTCs play an ancillary role in the diagnosis of pulmonary nodules.TP53 mutations in CTCs might be used to identify benign and malignant pulmonary nodules. | Changdan XU Xiaohong XU Weipeng SHAO Hongliang SUN Xiaohong LIU Hongxiang FENG Xianbo ZUO Jingyang GAO Guohui WANG Xiongtao YANG Runchuan GU Shutong GE Shijie WANG Liwei GAO Guangying ZHU | 2023 | 中国肺癌杂志2023，26，6： | 1 |
| 17 | Absorption characteristics of As( Ⅴ ) , Se ( Ⅳ ) , and V ( Ⅴ ) onto activated alumina: Effects of pH, surface loading, and ionic strength 显示文摘 | Su Tingzhi Guan Xiaohong Gu Guowei | 2008 | Journal of Colloid and Interface Science2008，326，： | 1 |
| 18 | Extraction, purification, a- nd characterization of the polysaeeharides from Opuntia milpaaLta 显示文摘 | Cai Weirong Gu Xiaohong Tang Jian | 2008 | Carbohydrate Polymers2008，71，3： | 1 |
| 19 | Effects of components in culture medium on glutamate decarboxylase activity and γ-aminobutyric acid accumulation in foxtail millet ( Setaria italica L.) during germination显示文摘 | Qingyun Bai Meiqing Chai Zhenxin Gu Xiaohong Cao Yan Li Kunlun Liu | 2009 | Food Chemistry2009，，1： | 1 |
| 20 | Isolation and characterization of a novel fucose-binding lectin from the gill of bighead carp ( Aristichthys nobilis )显示文摘 | Saikun Pan Jian Tang Xiaohong Gu | 2009 | Veterinary Immunology and Immunopathology2009，，2： | 1 |
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