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| 1 | Gene expression signatures for phlegm-dampness constitution of Chinese Medicine显示文摘Dear Editor,The term'individualized medicine'was first used in Biochemistry Individuality in 1956 by Williams(Williams et al.,1956).With the emergence of the post-genome era in the21st century,individualized precision medicine has attracted increasing attention.In traditional Chinese medicine(TCM),there is also concept of individualized medicine,which is known as the Chinese constitutional theory,proposed in the1970s(Wang,2012).According to this theory,the | Lingru Li Juan Feng Haiqiang Yao Lan Xie Yu Chen Lingling Yang Shujuan Hou Shipeng Zhao Ranran Sun Yanling Wu Tongtong Bai Yingshuai Li Ruoxi Yu Ji Wang Qi Wang | 2017 | Science China(Life Sciences)2017，60，1： | 13 |
| 2 | Plasma-induced nanowelding of a copper nanowire network and its application in transparent electrodes and stretchable conductors显示文摘铜 nanowires (Cu NW ) 由于他们的突出的电的性质和低费用为电子学作为积木吸引了增加的注意。然而,无所不在地在 Cu NW 的表面上存在的器官的残余和氧化物层阻碍好内部电线的接触。象热退火和酸处理那样的通常使用的方法经常导致 nanowire 损坏。此处,在房间温度的氢血浆处理被表明了为同时的表面在连接清洗并且选择焊接 Cu NW 有效。有优秀光电的性能的透明电极(19 O 汯獹獩琠浥数慲畴敲漠 ??? ‰ ???????璪?? | Ranran Wang Haitao Zhai Tao Wang Xiao Wang Yin Cheng Liangjing Shi Jing Sun | 2016 | Nano Research2016，9，7： | 11 |
| 3 | Efficacy and safety of Yinchenwuling powder for hyperlipidemia: a systematic review and Meta-analysis显示文摘OBJECTIVE:To assess the clinical effectiveness and adverse effects of Yinchenwuling powder(YCWLP)in the treatment of hyperlipidemia using Meta-analysis.METHODS:Seven electronic databases were searched for randomized controlled trials designed to evaluate the clinical effectiveness of YCWLP for hyperlipidemia published in any language prior to February 2015.Two reviewers independently identified articles,extracted data,assessed quality,and cross-checked the results.Revman 5.3 was used to analyze the data.RESULTS:Only five randomized controlled trials with poor methodology were included in the analy-sis.The five trials compared YCWLP with conventional lipid-lowering drugs.Meta-analysis indicated that YCWLP was more effective at the levels of total cholesterol and triglycerides,while increasing the level of high-density lipoprotein cholesterol without serious adverse effects.However,it was not more effective than lipid-lowering drugs in reducing low-density lipoprotein cholesterol and improving hemorheology.CONCLUSION:YCWLP appeared to improve lipid levels.However,given the high risk of bias among the trials,we could not conclude that YCWLP was beneficial to patients with hyperlipidemia.More rigorous trials are required to provide stronger evidence for the conclusion. | Yao Haiqiang Zhang Zengliang Wang Ji Zuo Jiacheng Chen Yu Zhu Libing Li Xiaoke Yang Zheng Wang Zisong Sun Ranran Xu Xuanxuan Li Changming Wu Yanling Li Lingru Wang Qi | 2016 | Journal of Traditional Chinese Medicine2016，36，2： | 11 |
| 4 | Novel fabrication of copper nanowire/cuprous oxidebased semiconductor-liquid junction solar cells显示文摘Cu nanowire (NW )/cuprous 氧化物(Cu 2 O ) 有极大地提高的效率和减少的费用的基于的半导体液体连接太阳能电池被装配。Cu NW 象 Cu NWs/ Cu 2 O 同轴的结构,它显著地有益于费用分离。最好的太阳能电池在模仿的 AM1.5G 照明下面象 1.92% 一样高到达了变换效率,它基于做氟的符号的听氧化物和 Cu 2 O。 | Haitao Zhai Ranran Wang Weiqi Wang Xiao Wang Yin Cheng Liangjing Shi Yangqiao Liu Jing Sun | 2015 | Nano Research2015，8，10： | 6 |
| 5 | Classification of traditional Chinese medicine constitution based on facial features in color images显示文摘Objective:To explore the possible correlation between traditional Chinese medicine(TCM)constitution and facial features in color images and to improve the accuracy of automated constitution classification.Methods:Color images were taken of 5150 individuals of different professions.Automated face detection and key point positioning were performed on the collected images,which were then transformed into a standard size.The relationship between facial features and TCM constitution based on the red,green,blue(RGB)pixel and the local binary pattern(LBP)texture features was explored.Results:The overall accuracy rate and robustness of TCM constitution classification based on RGB features were low.Classification results of the phlegm-dampness,damp-heat,blood stasis,and balance constitutions achieved high accuracy rates.Classification accuracy rate using the LBP texture feature was higher than that of the RGB feature,with the best accuracy observed for the balance constitution.Conclusion:Application of computer image acquisition and processing of facial features may serve as an adjunct to the TCM diagnostic method of inspection. | Jian Zhang Shujuan Hou Ji Wang Lingru Li Pin Li Junwen Han Haiqiang Yao Ranran Sun Ziqing Li Zhen Lei Qi Wang | 2016 | Journal of Traditional Chinese Medical Sciences2016，3，3： | 5 |
| 6 | Transparent heaters based on highly stable Cu nanowire films显示文摘尽管有灵活、透明的电影加热器的最近的成功的示范,有高光的发射度和低应用直接电流(DC ) 的大多数加热器电压是基于的银 nanowire (Ag NW ) 或银基于格子。在这研究,灵活、可拉长的铜 nanowire (Cu NW ) 基于透明电影加热器通过一个基于答案的过程,恐水病的聚合物的薄层在 Cu NW 电影上在被包含被制作。薄聚合物层在严厉严峻的条件下面保护了这些电影免受氧化的伤害,即,高温度,高湿度,和酸、碱的环境。这些电影展出了显著性能,一个宽操作温度范围(多达 150 | Haitao Zhai Ranran Wang Xiao Wang Yin Cheng Liangjing Shi Jing Sun | 2016 | Nano Research2016，9，12： | 5 |
| 7 | Semi-transparent polymer solar cells with all-copper nanowire electrodes显示文摘透明电极包括高光的发射度,好传导性,和优秀机械灵活性由于他们的优点基于铜 nanowires (Cu NW ) 吸引了重要注意。然而,有 all-Cu NW 电极的便宜、高效、环境的友好太阳能电池没被认识到直到现在。此处,顶和底部透明电极基于有低表面粗糙和同类的电导率的 Cu NW 被制作。然后,有 polyacrylate/Cu NWs/poly (3,4-ethylenedioxythiophene ) 的转换结构的半透明的聚合物太阳能电池(PSC ): poly (styrenesulfonate )(PEDOT : PSS )(PH1000 )/Y-TiO 2/poly(3-hexylthiophene):[6,6]-phenyl-C61-butyric 酸 3,4,5-tris (octyloxy ) benzyl/PEDOT : PSS (4083 )/Cu NWs/polyimide/polydimethylsiloxane 被构造;这些能与到达 1.97% 和 1.85% 的力量变换效率从双方吸收光。而且, PSC 在可见区域显示出 42% 的平均发射度,它为象产生力量的窗口和大楼综合的 photovoltaics 那样的一些专业化应用程序使他们变为合适。铟听氧化物(ITO )- 并且高贵没有金属的 PSC 能为制作划算的半透明的 PSC 铺新小径。 | Haitao Zhai Yang Li Liwei Chen Xiao Wang Liangjing Shi Ranran Wang Jing Sun | 2018 | Nano Research2018，11，4： | 4 |
| 8 | Copper nanowire-TiO2-polyacrylate composite electrodes with high conductivity and smoothness for flexible polymer solar cells显示文摘 | Haitao Zhai Yang Li Liwei Chen Xiao Wang Liangjing Shi Ranran Wang Jing Sun | 2018 | Nano Research2018，11，4： | 2 |
| 9 | EGFR/BRAF/MEK co-inhibition for EGFR-mutated lung adenocarcinoma patients with an acquired BRAF^(V600E) mutation: a case report and review of literature显示文摘Despite the promising initial anti-tumor efficacy of epidermal growth factor receptor-tyrosine kinase inhibitors(EGFR-TKIs),most advanced non-small-cell lung cancers(NSCLCs)progress eventually due to therapeutic resistance.V-Raf murine sarcoma viral oncogene homolog B1(BRAF)^(V600E) mutation has been considered as an uncommon mutation that contributes to acquired resistance for EGFR-TKIs.In the presented case,BRAF^(V600E) mutation was detected as an acquired resistance-mediated mutation in a patient treated with osimertinib(a third-generation EGFR-TKI).The presented patient achieved partial regression and ongoing PFS of four months after the co-inhibition of osimertinib plus dabrafenib(BRAF inhibitor)and trametinib(MEK inhibitor).Our case further enriches the clinical evidence of the efficacy of EGFR/BRAF/MEK co-inhibition in patients with an acquired BRAF^(V600E) mutation,consistent with the review of the literature(eight cases).Additionally,our case highlights the important role of sample type,method,and platform of gene detection in patient management,life quality,and prognosis,as well as the understanding of acquired resistance mechanism. | Ran Zeng Lifeng Luo Xianwen Sun Zhiyao Bao Wei Du Ranran Dai Wei Tang Beili Gao Yi Xiang | 2021 | Cancer Drug Resistance2021，4，4： | 2 |
| 10 | General synthesis of high-performing magneto-conjugated polymer core-shell nanoparticles for multifunctional theranostics显示文摘最近,增加注意作为 theranostic 平台被付了到结合磁电机的聚合物核心壳 nanoparticles (NP ) 。然而,表面活化剂的利用和有在合成的潜在的毒性的额外的氧化剂,为磁性的 NP ( MNP )@的各种各样的类型的控制合成的一般方法的缺乏结合了聚合物 NP ,并且获得平衡磁电机光的性质的困难极大地在 theranostics 限制了结合磁电机的聚合物的应用。我们发展了一在里面没有额外的表面活化剂和氧化剂的 situ 表面聚合方法综合有平衡、突出的磁电机光的性质的 MNP@polypyrrole (PPy ) NP。有一种可调节的尺寸,不同形状,和控制的壳厚度的 MNP@PPy NP 用这个方法被获得。方法被扩大综合另外的结合 MNP 的聚合物核心壳 NP,例如 MNP@polyaniline 并且 MNP@poly (3,4-ethylenedioxythiophene ): poly (4-styrenesulfonate )(PEDOT : PSS ) 。我们根据我们的试验性的结果讨论建议方法的形成机制。最后,使用光并且获得的 MNP@PEDOT 的磁性: PSS NP,在 vivo,多模式的指导成像的过高热在老鼠被导致,完成优秀肿瘤脱离治疗学的效果。我们的工作为在生物医学的域里扩大结合 MNP 的聚合物核心壳 NP 的应用程序是有益的。 | Hao Yan Lingyun Zhao Wenting Shang Zhongqun Liu Wensheng Xie Cai Qiang Zhiyuan Xiong Ranran Zhang Baohua Li Xiaodan Sun Feiyu Kang | 2017 | Nano Research2017，10，2： | 2 |
| 11 | A rule to distin- guish diastereomerie bianthrones bylH NMR显示文摘 | JI Naiyun LIANG Xiaorui SUN Ranran | 2014 | RSC Ad- vances2014，4，： | 1 |
| 12 | Ferromagnetic and ferroelectric properties of Aurivillius phase Bi_9Fe_(4.7)Me_(0.3)Ti_3O_(27)(Me 5 Fe, Co, Ni, Mn)显示文摘New Aurivillius phase Bi9Fe4.7Me0.3Ti3O27(Me = Fe, Co, Ni, Mn) oxides have been prepared using a citrate combustion method. X-ray diffraction on powders and high-resolution transmission electron microscopy investigation confirmed that the Bi9Fe4.7Me0.3Ti3O27 samples are with an eight-layer structure. Both ferromagnetic and ferroelectric investigations suggested that Co or Ni substitution could enhance their multiferroic properties,while Mn substitution depressed them. Among all the samples, Bi9Fe4.7Co0.3Ti3O27 sample exhibits the largest remnant polarization of Pr*3.8 l C/cm2, and the largest remnant magnetization of Mr*0.06 lB/f.u. with a Curie temperature about 764 K, while the Bi9Fe4.7Ni0.3Ti3O27 sample has the largest spontaneous magnetization(0.26 lB/f.u.). The improved ferromagnetic properties ofboth Bi9Fe4.7Co0.3Ti3O27 and Bi9Fe4.7Ni0.3Ti3O27 can be ascribed to the spin canting of magnetic ion-based sublattices via the Dzyaloshinskii–Moriya interaction and also the magnetic ions exchanging interactions(Fe3–O–Co3or Fe3–O–Ni3). | Guopeng Wang Shujie Sun Yan Huang Jianlin Wang Ranran Peng Zhengping Fu Yalin Lu | 2014 | Chinese Science Bulletin2014，59，36： | 1 |
| 13 | Mechanical and biocompatible properties of polymer-infiltrated-ceramic-network materials for dental restoration显示文摘Dental restorative materials with high mechanical properties and biocompatible performances are promising.In this work,polymer-infiltrated-ceramic-network materials(PICNs)were fabricated via infiltrating polymerizable monomers into porous ceramic networks and incorporated with hydroxyapatite nano-powders.Our results revealed that the flexural strength can be enhanced up to 157.32 MPa,and elastic modulus and Vickers hardness can be achieved up to 19.4 and 1.31 GPa,respectively,which are comparable with the commercial computer-aided design and computer-aided manufacturing(CAD/CAM)blocks.Additionally,the adhesion and spreading of rat bone marrow mesenchymal stem cells(r BMSCs)on the surface of such materials can be improved by adding hydroxyapatite,which results in good biocompatibility.Such PICNs are potential applicants for their application in the dental restoration. | Bencang CUI Ranran ZHANG Fengbo SUN Qian DING Yuanhua LIN Lei ZHANG Cewen NAN | 2020 | Journal of Advanced Ceramics2020，9，1： | 1 |
| 14 | Dominant changes in the breast muscle lipid profiles of broiler chickens with wooden breast syndrome revealed by lipidomics analyses显示文摘Background: Chicken is the most consumed meat worldwide and the industry has been facing challenging myopathies. Wooden breast(WB), which is often accompanied by white striping(WS), is a serious myopathy adversely affecting meat quality of breast muscles. The underlying lipid metabolic mechanism of WB affected broilers is not fully understood.Results: A total of 150 chickens of a white-feathered, fast-growing pure line were raised and used for the selection of WB, WB + WS and control chickens. The lipids of the breast muscle, liver, and serum from different chickens were extracted and measured using ultra performance liquid chromatography(UPLC) plus Q-Exactive Orbitrap tandem mass spectrometry. In the breast, 560 lipid molecules were identified. Compared to controls, 225/225 of 560 lipid molecules(40.2%) were identified with differential abundance(DA), including 92/100 significantly increased neutral lipids and 107/98 decreased phospholipids in the WB/WB + WS groups, respectively. The content of monounsaturated fatty acids(MUFA) was significantly higher, and the polyunsaturated fatty acids(PUFA) and saturated fatty acids(SFA) were significantly lower in the affected breasts. In the liver, 434 lipid molecules were identified, and 39/61 DA lipid molecules(6.7%/14.1%) were detected in the WB and WB + WS groups, respectively. In the serum, a total of 529 lipid molecules were identified and 4/44 DA lipid molecules(0.8%/8.3%) were detected in WB and WB + WS group, respectively. Compared to controls, the content of MUFAs in the serum and breast of the WB + WS group were both significantly increased, and the content of SFAs in two tissues were both significantly decreased. Only five lipid molecules were consistently increased in both liver and serum in WB + WS group.Conclusions: We have found for the first time that the dominant lipid profile alterations occurred in the affected breast muscle. The relative abundance of 40.2% of lipid molecules were changed and is characteristic of increased neutral lipids and decreased phospholipids in the affected breasts. Minor changes of lipid profiles in the liver and serum of the affected groups were founded. Comprehensive analysis of body lipid metabolism indicated that the abnormal lipid profile of WB breast may be independent of the liver metabolism. | Ranran Liu Fuli Kong Siyuan Xing Zhengxiao He Lu Bai Jiahong Sun Xiaodong Tan Di Zhao Guiping Zhao Jie Wen | 2022 | Journal of Animal Science and Biotechnology2022，13，6： | 1 |
| 15 | The First High-quality Reference Genome of Sika Deer Provides Insights into High-tannin Adaptation显示文摘Sika deer are known to prefer oak leaves,which are rich in tannins and toxic to most mammals;however,the genetic mechanisms underlying their unique ability to adapt to living in the jungle are still unclear.In identifying the mechanism responsible for the tolerance of a highly toxic diet,we have made a major advancement by explaining the genome of sika deer.We generated the first high-quality,chromosome-level genome assembly of sika deer and measured the correlation between tannin intake and RNA expression in 15 tissues through 180 experiments.Comparative genome analyses showed that the UGT and CYP gene families are functionally involved in the adaptation of sika deer to high-tannin food,especially the expansion of the UGT family 2 subfamily B of UGT genes.The first chromosome-level assembly and genetic characterization of the tolerance to a highly toxic diet suggest that the sika deer genome may serve as an essential resource for understanding evolutionary events and tannin adaptation.Our study provides a paradigm of comparative expressive genomics that can be applied to the study of unique biological features in non-model animals. | Xiumei Xing Cheng Ai Tianjiao Wang Yang Li Huitao Liu Pengfei Hu Guiwu Wang Huamiao Liu Hongliang Wang Ranran Zhang Junjun Zheng Xiaobo Wang Lei Wang Yuxiao Chang Qian Qian Jinghua Yu Lixin Tang Shigang Wu Xiujuan Shao Alun Li Peng Cui Wei Zhan Sheng Zhao Zhichao Wu Xiqun Shao Yimeng Dong Min Rong Yihong Tan Xuezhe Cui Shuzhuo Chang Xingchao Song Tongao Yang Limin Sun Yan Ju Pei Zhao Huanhuan Fan Ying Liu Xinhui Wang Wanyun Yang Min Yang Tao Wei Shanshan Song Jiaping Xu Zhigang Yue Qiqi Liang Chunyi Li Jue Ruan Fuhe Yang | 2023 | Genomics, Proteomics & Bioinformatics2023，21，1： | 1 |
| 16 | Chicken gga-miR-1306-5p targets Tollip and plays an important role in host response against Salmonella enteritidis infection显示文摘Background: Increasing evidence indicates that micro RNAs(mi RNAs) are involved in inflammatory response and immune regulation following pathogen invasion. The purpose of this study was to elucidate the roles played by Gallus gallus micro RNA-1306-5 p(gga-mi R-1306-5 p) in host responses against potential invasion by Salmonella enteritidis(SE) in chickens and the underlying mechanisms.Results: In present study, the expression levels of gga-mi R-1306-5 p were determined in both tissues and HD11 cells. The results showed that gga-mi R-1306-5 p was significantly increased following SE infection or lipopolysaccharide(LPS) stimulation. The dual luciferase reporter assay further validated that gga-mi R-1306-5 p targeted the Toll-interacting protein(Tollip), and thereby participated in the regulation of immune response against SE or LPS stimulation through binding with the 3′-untranslated region(3’UTR) of Tollip. Additionally, the expression of Tollip was significantly blocked by over-expressed gga-mi R-1306-5 p. The underlying mechanisms by which ggami R-1306-5 p modulated the production of pro-inflammatory cytokines were also investigated. Molecular biological assays demonstrated that overexpression of gga-mi R-1306-5 p promoted the production of pro-inflammatory mediators, including NF-κB, TNF-α, IL-6, and IL-1β, which produced effects similar to those of Tollip knockdown.Conclusions: Taken together, gga-mi R-1306-5 p induced by SE or LPS, regulates the immune response by inhibiting Tollip, which activates the production of inflammatory cytokines. This study has provided the first direct evidence that gga-mi R-1306-5 p targets Tollip, and is involved in the host response against SE. | Weiwei Sun Ranran Liu Peng Li Qinghe Li Huanxian Cui Maiqing Zheng Jie Wen Guiping Zhao | 2019 | Journal of Animal Science and Biotechnology2019，10，4： | 1 |
| 17 | A unified thermal model of thermophysical effects with pulse width from nanosecond to femtosecond 显示文摘 | Fang Ranran Zhang Duanming Wei Hua Li Zhihua Yang Fengxia Gao Yihua Hu Dezhi Li Zhihua Tan Xinyu Sun Min Yang Fengxia | 2008 | The European Physical Journal Applied Physics2008，42，3： | 1 |
| 18 | Effect of dendritic cell/cytokine-induced killer cell immunobiological cancer therapy combined with adjuvant chemotherapy in patients with triple-negative breast cancer显示文摘Objective The aim of the present study was to investigate the effect of dendritic cell(DC)/cytokine-induced killer cell(CIK) immunobiological cancer therapy in patients with triple-negative breast cancer(TNBC) who underwent adjuvant chemotherapy. Methods From January 2010 to October 2013, 120 patients with postoperative TNBC were recruited and included in the study. Patients were enrolled in one of two groups according to whether they accepted DC/CIK immunobiological cancer therapy during adjuvant chemotherapy; the patients in the DC/CIK group underwent adjuvant chemotherapy combined with DC/CIK immunobiological cancer therapy, and the control group underwent adjuvant chemotherapy alone. When six cycles of adjuvant chemotherapy and six cycles of DC/CIK immunobiological cancer therapy had been completed, differences between the two groups with regard to quality of life(Qo L), immunological indicators(CD3, CD4, CD8, and NK cell levels), disease-free survival(DFS), and side effects of chemotherapy and DC/CIK treatment were evaluated.Results In the DC/CIK group, the proportion of NK cells and CD3+ and CD4+ T-cell subgroups significantly increased, and the proportion of CD8+ cells decreased when they were compared before and after DC/CIK therapy(P < 0.05). However, there were no significant changes in the control group. By the final follow-up, DFS of the treatment group and the control group was 38.4 and 34.2 months, respectively. The Qo L improved in the patients treated with chemotherapy plus DC/CIK therapy compared with the patients treated with chemotherapy alone, and the difference between groups was significant(P < 0.05). The side effects of two groups were tolerable and not significantly different between the two groups.Conclusion The DC/CIK treatment had potential benefits for patients with TNBC compared with the control group, and was not associated with any obvious side effects. Therefore, DC/CIK therapy is a safe and effective method for the treatment of TNBC. | Ranran Zhang Wanqing Xie Tao Han Yongye Liu Zhaozhe Liu Fang Guo Yaling Han Zhenyu Ding Yinghui Sun Dongchu Ma Xiaodong Xie | 2015 | The Chinese-German Journal of Clinical Oncology2015，14，1： | 1 |
| 19 | Development of an automatic measurement system using atmospheric pressure photoionization ultrahigh-resolution mass spectrometry and application for on-line analysis of particulate matter显示文摘On-line chemical characterization of atmospheric particulate matter(PM)with soft ionization technique and ultrahigh-resolution Mass Spectrometry(UHRMS)provides molecular information of organic constituents in real time.Here we describe the development and application of an automatic measurement system that incorporates PM_(2.5)sampling,thermal desorption,atmospheric pressure photoionization,and UHRMS analysis.Molecular formulas of detected organic compounds were deducted from the accurate(±10 ppm)molecular weights obtained at a mass resolution of 100,000,allowing the identification of small organic compounds in PM_(2.5).Detection efficiencies of 28 standard compounds were determined and we found a high sensitivity and selectivity towards organic amines with limits of detection below 10 pg.As a proof of principle,PM_(2.5)samples collected off-line in winter in the urban area of Beijing were analyzed using the Ionization Module and HRMS of the system.The automatic system was then applied to conduct on-line measurements during the summer time at a time resolution of 2 hr.The detected organic compounds comprised mainly CHON and CHN compounds below 350 m/z.Pronounced seasonal variations in elemental composition were observed with shorter carbon backbones and higher O/C ratios in summer than that in winter.This result is consistent with stronger photochemical reactions and thus a higher oxidation state of organics in summer.Diurnal variation in signal intensity of each formula provides crucial information to reveal its source and formation pathway.In summary,the automatic measurement system serves as an important tool for the on-line characterization and identification of organic species in PM_(2.5). | Yayuan Dong Ranran Liu Ling Xie Xiaole Pan Yele Sun Lin Wu ZifaWang | 2024 | Journal of Environmental Sciences2024，，4： | 0 |
| 20 | Molecular Cloning,Characterization and Expression Profile of Myf5 and Myf6 During Growth and Development in the Seriola lalandi显示文摘Myogenic Regulatory Factors(MRFs)is involved in the muscle growth and differentiation.In this study,the cDNA sequence of yellowtail kingfish MRFs genes were cloned by rapid amplification of cDNA ends(RACE)method;then,the character-istics of these genes and the predicted protein sequences were analyzed by bioinformatics methods,the tissue and embryonic stages differential expression pattern were detected by the quantitative real-time PCR.Our results showed that the yellowtail kingfish(YTK)Myf5 cDNA has a full length of 951 bp,encoding 266 amino acids.The yellowtail kingfish Myf6 cDNA has a full length of 1105bp,encoding 250 amino acids.The proteins containα-helix,β-strand,and loops.The Neighbour-joining tree revealed that YTK Myf5 and Myf6 are closely related to Seriola dumerili.The yellowtail kingfish Myf5 and Myf6 gene expressed significantly higher in mus-cle than in other tissues(P<0.05).In addition,Myf5 and Myf6 in muscle was significantly expressed in 400g and 500 g fish but not in 50 g,suggesting that myogenic regulatory factors expression had a great relationship with the fish size.Our results also indicated that Myf5 and Myf6 have different functions during embryonic development,because Myf5 showed highest expression level at the neuroembryo period,but Myf6 had the highest expression level at embryo coverage yolk 70%stage.Myf5 gene showed highest ex-pression at 30 d of age,suggesting it played key roles in myogenic period.However,the Myf6 gene was significantly highly ex-pressed at 60 d,revealing this gene functioned in the later muscle formation period. | SHI Bao SUN Ranran LIU Xuezhou ZHANG Zhengrong XU Yongjiang JIANG Yan WANG Bin | 2021 | Journal of Ocean University of China2021，20，6： | 0 |
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