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| 1 | Hantavirus infection:a global zoonotic challenge显示文摘Hantaviruses are comprised of tri-segmented negative sense single-stranded RNA, and are members of the Bunyaviridae family. Hantaviruses are distributed worldwide and are important zoonotic pathogens that can have severe adverse effects in humans. They are naturally maintained in specific reservoir hosts without inducing symptomatic infection. In humans, however,hantaviruses often cause two acute febrile diseases, hemorrhagic fever with renal syndrome(HFRS) and hantavirus cardiopulmonary syndrome(HCPS). In this paper, we review the epidemiology and epizootiology of hantavirus infections worldwide. | Hong Jiang Xuyang Zheng Limei Wang Hong Du Pingzhong Wang Xuefan Bai | 2017 | Virologica Sinica2017，32，1： | 47 |
| 2 | Structural insight into substrate specificity of human intestinal maltase-glucoamylase显示文摘Human maltase-glucoamylase(MGAM)hydrolyzes linear alpha-1,4-linked oligosaccharide substrates,playing a crucial role in the production of glucose in the human lumen and acting as an efficient drug target for type 2 diabetes and obesity.The amino-and carboxyl-terminal portions of MGAM(MGAM-N and MGAM-C)carry out the same catalytic reaction but have different substrate specificities.In this study,we report crystal structures of MGAM-C alone at a resolution of 3.1Å,and in complex with its inhibitor acarbose at a resolution of 2.9Å.Structural studies,combined with biochemical analysis,revealed that a segment of 21 amino acids in the active site of MGAM-C forms additional sugar subsites(+2 and+3 subsites),accounting for the preference for longer substrates of MAGM-C compared with that of MGAM-N.Moreover,we discovered that a single mutation of Trp1251 to tyrosine in MGAM-C imparts a novel catalytic ability to digest branched alpha-1,6-linked oligosaccharides.These results provide important information for understanding the substrate specificity of alphaglucosidases during the process of terminal starch digestion,and for designing more efficient drugs to control type 2 diabetes or obesity. | Limei Ren Xiaohong Qin Xiaofang Cao LeleWang Fang Bai Gang Bai Yuequan Shen | 2011 | Protein & Cell2011，2，10： | 9 |
| 3 | Numerical simulation and processoptimization for producing large-sized castings显示文摘3-Dvelocity and temperature fields of mold filling and solidification processes of large-sized castingswere calculated,and the efficiency and accuracy of numerical calculation were studied.The mold filling andsolidification processes of large-sized stainless steel,iron and aluminum alloy castings were simulated by using ofnew scheme;their casting processes were optimized,and then applied to produce castings. | Wang Junqing Sun Xun Guan Yang Wang Penghua Li Hailan Bai Limei Sun Xinzhi | 2008 | China Foundry2008，5，3： | 6 |
| 4 | Natural variations of ZmSRO1d modulate the trade-off between drought resistance and yield by affecting ZmRBOHC-mediated stomatal ROS production in maize显示文摘While crop yields have historically increased,drought resistance has become a major concern in the context of global climate change.The trade-off between crop yield and drought resistance is a common phenomenon;however,the underlying molecular modulators remain undetermined.Through genome-wide association study,we revealed that three non-synonymous variants in a drought-resistant allele of ZmSRO1d-R resulted in plasma membrane localization and enhanced mono-ADP-ribosyltransferase activity of ZmSRO1d toward ZmRBOHC,which increased reactive oxygen species(ROS)levels in guard cells and promoted stomatal closure.ZmSRO1d-R enhanced plant drought resilience and protected grain yields under drought conditions,but it led to yield drag under favorable conditions.In contrast,loss-of-function mutants of ZmRBOHC showed remarkably increased yields under well-watered conditions,whereas they showed compromised drought resistance.Interestingly,by analyzing 189 teosinte accessions,we found that the ZmSRO1d-R allele was present in teosinte but was selected against during maize domestication and modern breeding.Collectively,our work suggests that the allele frequency reduction of ZmSRO1d-R in breeding programs may have compromised maize drought resistance while increased yields.Therefore,introduction of the ZmSRO1d-R allele into modern maize cultivars would contribute to food security under drought stress caused by global climate change. | Huajian Gao Junjun Cui Shengxue Liu Shuhui Wang Yongyan Lian Yunting Bai Tengfei Zhu Haohao Wu Yijie Wang Shiping Yang Xuefeng Li Junhong Zhuang Limei Chen Zhizhong Gong Feng Qin | 2022 | Molecular Plant2022，15，10： | 2 |
| 5 | Expression,purification and characterization of Mycobacterium tuberculosis RpfE protein显示文摘Resuscitation promoting factor E(RpfE)is one of the five Rpf-like proteins in Mycobacterium tuberculosis(M.tuberculosis).These Rpf-like proteins are secretory,which make them candidates for recognition by the host immune system.In this study,the RpfE gene was amplified from M.tuberculosis,cloned into the expression vectors pDE22 and pPRO EXHT,and were expressed in Mycobacterium vaccae(M.vaccae)and Escherichia coli DH5α,respectively.Both recombinant RpfE proteins were purified by Ni-Sepharose affinity chromatography,and were given to C57BL/6 mice.The RpfE proteins elicited T cell proliferation,and stimulated the production of gamma interferon(IFN-γ),interleukin-10(IL-10)and IL-12.Our results indicated that the RpfE protein expressed in M.vaccae could more efficiently stimulate cellular immune response,making it a promising candidate as a subunit vaccine. | Ying Xue Yinlan Bai Xue Gao Hong Jiang Limei Wang Hui Gao Zhikai Xu | 2012 | The Journal of Biomedical Research2012，26，1： | 2 |
| 6 | Synthesis, crystal structure and photocatalytic performance of polyoxometalate K_(13)[Gd(GeW_(11)O_(39))_2]·34H_2O显示文摘A polyoxometalate K_(13)[Gd(GeW_(11)O_(39))_2]·34 H_2 O was synthesized and characterized by elemental analysis,IR spectroscopy, UV spectra, XRD and thermal gravimetric analysis. X-ray single-crystal structural analysis indicates that the title compound crystallizes in the triclinic crystal system. The heteropolyanion of [Gd(GeW_(11)O_(39))_2]^(13) consists of two [GeW_(11)O_(39)]^(8-) vacant Keggin moieties linked via Gd^(3+). Photocatalytic performance of K_(13)[Gd(GeW_(11)O_(39))_2]·34 H_2 O in photodegradation of X-3 B was also studied.When the reaction time is 180 min, the decoloring rate reaches 71.19%. With the decrease of pH, the decolorization rate increases gradually. | Haibin Liu Lin Bai Limei Ai Wenshuang Dai Danfeng Zhang Qingyin Wu Renchun Zhang | 2019 | Journal of Rare Earths2019，37，6： | 1 |
| 7 | Effect of esculetin on bone metabolism in ovariectomized rats显示文摘OBJECTIVE: To determine the effect of an esculetin formulation(at 97.4% purity) on osteoporosis, and to investigate the potential underlying molecular mechanism(s).METHODS: Sixty specific pathogen free-grade female Wistar rats were randomly assigned to three groups: blank control(n = 12), sham(n = 12), and model(n = 36). The model group were bilaterally ovariectomized. The sham group had the tissue surrounding the ovaries removed, while the ovaries were retained. After 3 months, the model group was randomly divided into three subgroups: OVX(n = 12), positive control(n = 12), and esculetin(n =12). The positive control group and the esculetin group were intragastrically administered diethylstilbestrol(0.046 mgkd^(-1)), respec·kg^(-1)tively,·d^(-1)) or esculetin(384 mgg^(-1) once per day for 6 consecu··-tive days; medication administration was then stopped for 1 d, before being administered for another 6 consecutive days. All rats were treated for 3months. Samples were collected at the end of the treatment period. An Osteocore3 Digital 2D bone densitometer was used to test the bone mineral density, and histomorphometric analysis was performed to measure bone mass, bone formation,and bone resorption. Enzyme-linked immunosorbent assay analysis was used to measure the serum concentrations of interleukin-6(IL-6), osteoprotegerin(OPG), and receptor activator of nuclear factor-kappa B ligand(RANKL). Immunohistochemistry and in situ hybridization were performed to detect the protein and mRNA expressions of OPG and RANKL in osteoblasts and bone marrow stromal cells.RESULTS: Compared with the OVX group, the esculetin group had significantly greater femoral bone mineral density and tibial trabecular bone volume,and significantly smaller trabecular resorption surface. The percentage of trabecular formation surface, average osteoid width, trabecular bone mineralization rate, and cortical bone mineralization rate did not significantly differ between groups. Compared with the sham group, the esculetin group had significantly decreased serum levels of IL-6and RANKL, and significant downregulation of RANKL protein and mRNA expression levels in osteoblasts and bone marrow stromal cells; however, there was no significant difference between groups in OPG.CONCLUSION: Esculetin can increase bone mass by upregulating RANKL expression in osteoblasts and bone marrow stromal cells, and decreasing serum IL-6 concentration. This indicates that the therapeutic effect of esculetin on osteoporosis occurs via decreased bone resorption. | Liu Meijie Wang Ruihai Li Yan Bai Dong Pan Jinghua Liu Hong Wang Shaojun Wu Jiaying Sun Gang Miao Qing Ju Dahong Liu Limei | 2018 | Journal of Traditional Chinese Medicine2018，38，6： | 1 |
| 8 | Immunogenicity and protective efficacy of a DNA vaccine encoding the fusion protein of mycobacterium heat shock protein 65 with interleukin-2 against Mycobacterium tuberculosis in BALB/c mice 显示文摘 | Limei Wang Yinlan Bai Changhong Shi | 2008 | APMIS2008，116，： | 1 |
| 9 | The Suggestive Effects of Symbolism ——Writing Skills In Sister Carrie显示文摘This article gives a brief analysis of an illustration of symbolism-one of Theodore Dreiser’s writing skills applied in his Sister Carrie. The application of symbolism provides some suggestive effects to this novel, that is, it helps to establish the elements, reveal the reality and strengthen the themes, portray characters and buttress the concept, heighten the impact, etc. | HT5SS]Yang Lili 1 Bai Limei 2 (1 Zhangye Normal College, Zhangye,734000 2.Northwest Normal University, Lanzhou, 730070, China) | 2000 | 兰州大学学报(社会科学版)2000，28，S1： | 0 |
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