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| 1 | Risk factors for cervical lymph node metastasis in papillary thyroid microcarcinoma: a study of 1,587 patients显示文摘Objective: The purposes of this study were to identify risk factors for cervical lymph node metastasis and to examine the association between BRAF^(V600E) status and clinical features in papillary thyroid microcarcinoma(PTMC).Methods: A total of 1,587 patients with PTMC, treated in Tianjin Medical University Cancer Institute and Hospital from January2011 to March 2013, underwent retrospective analysis. We reviewed and analyzed factors including clinical results, pathology records, ultrasound results, and BRAF^(V600E) status.Results: Multivariate logistic regression analyses demonstrated that gender(male) [odds ratio(OR) = 1.845, P = 0.000], age(< 45 years)(OR = 1.606, P = 0.000), tumor size(> 6 mm)(OR = 2.137, P = 0.000), bilateralism(OR = 2.011, P = 0.000) and extrathyroidal extension(OR = 1.555, P = 0.001) served as independent predictors of central lymph node metastasis(CLNM).Moreover, CLNM(OR = 29.354, P = 0.000) served as an independent predictor of lateral lymph node metastasis(LLNM). Among patients with a solitary primary tumor, those with tumor location in the lower third of the thyroid lobe or the isthmus were more likely to experience CLNM(P < 0.05). Univariate analyses indicated that CLNM, LLNM, extrathyroidal extension, and multifocality were not significantly associated with BRAF^(V600E) mutation.Conclusions: The present study suggested that prophylactic neck dissection of the central compartment should be considered in patients with PTMC, particularly in men with tumor size greater than 6 mm, age less than 45 years, extrathyroidal extension, and tumor bilaterality. Among patients with PTMC, BRAF^(V600E) mutation is not significantly associated with prognostic factors. For a better understanding of surgical management of PTMC and the risk factors, we recommend multicenter research and long-term follow-up. | Xiangqian Zheng Chen Peng Ming Gao Jingtai Zhi Xiukun Hou Jingzhu Zhao Xi Wei Jiadong Chi Dapeng Li Biyun Qian | 2019 | Cancer Biology & Medicine2019，16，1： | 54 |
| 2 | Ginkgo biloba extract improved cognitive and neurological functions of acute ischaemic stroke:a randomised controlled trial显示文摘Purpose To evaluate the efficacy and safety of Ginkgo biloba extract(GBE)in acute ischaemic stroke and its impact on the recurrence of vascular events.Methods We conducted a multicentre,prospective,randomised,open label,blinded,controlled clinical trial enrollingpatients with an onset of acute stroke within 7 days from five hospitals in China Jiangsu Province.Participants were assigned to the GBE group(450 mg GBE with 100 mg aspirin daily)or the control group(100 mg aspirin daily)for 6 months.The primary outcome was the decline in the Montreal Cognitive Assessment score at 6 months.Secondary outcomes were other neuropsychological tests of cognitive and neurological function,the the incidence of adverse events and vascular events.results 348 patients were enrolled:179 in the GBE group and 169 in the control group.With 18 patients lost to follow-up,the dropout rate was 5.17%.Admission data between two groups were similar,but in the GBE group there was a marked slow down in the decline in the Montreal Cognitive Assessment scores(−2.77±0.21 vs−1.99±0.23,P=0.0116(30 days);−3.34±0.24 vs−2.48±0.26,P=0.0165(90 days);−4.00±0.26 vs−2.71±0.26,P=0.0004(180 days))compared with controls.The National Institutes of Health Stroke Scale scores at 12 and 30 days,the modified Rankin Scale scores for independent rate at 30,90 and 180 days,and the Barthel Index scores at 30,90 and 180 days in the GBE group were significantly improved compared with controls.Improvements were also observedin GBE groups for Mini-Metal State Examination scores of 30,90 and 180 days,Webster's digit symbol test scores at 30 days and Executive Dysfunction Index scores at 30 and 180 days.No significant differences were seen in the incidence of adverse events or vascular events.conclusions We conclude that GBE in combination with aspirin treatment alleviated cognitive and neurological deficits after acute ischaemic stroke without increasing the incidence of vascular events. | Shanshan Li Xinjiang Zhang Qi Fang Junshan Zhou Meijuan Zhang Hui Wang Yan Chen Biyun Xu Yanfeng Wu Lai Qian Yun Xu | 2017 | Stroke & Vascular Neurology2017，2，4： | 25 |
| 3 | Diagnosis of pancreatic carcinoma based on combined measurement of multiple serum tumor markers using artificial neural network analysis显示文摘 | Yang Yingchi Chen Hui Wang Dong Luo Wei Zhu Biyun Zhang Zhongtao | 2014 | Chinese Medical Journal2014，，10： | 15 |
| 4 | Detection of DNA methylation changes during seed germination in rapeseed (Brassica napus)显示文摘DNA methylation is known to play a crucial role in regulating plant development and or- gan or tissue differentiation. In this study, we as- sessed the extent and pattern of cytosine methylation during rapeseed (Brassica napus L.) seed germina- tion, and compared the methylation level of various tissues in seedling, using the techniques of methyla- tion-sensitive amplified polymorphism (MSAP) and HPLC separation and quantification of nucleosides. In all, 484 bands, each representing a recognition site cleaved by either or both of the isoschizomers, were amplified by 12 pairs of selective primers in DNA ob- tained from dry seeds. A total of 76 sites were found to be differentially digested by the isoschizomers, indicating that approximately 15.7% of 5′-CCGG-3′ sites in the genome were cytosine methylated. Four classes of patterns were observed in a comparative assay of cytosine methylation in the dry and germi- nating seeds; a small number of hypermethylation events occurred at 5′-CCGG-3′ sites in germinating seeds compared with dry seeds, while many more hypomethylation events were detected after seed germination. Differences in DNA methylation level in various tissues were also detected; radicel was less methylated than hypocotyl and cotyledon. These observations were further confirmed by HPLC analy- sis. In addition, sequencing of eleven differentially methylated fragments and the subsequent blast search revealed that cytosine methylated 5′-CCGG- 3′ sequences were equally distributed between cod- ing and non-coding regions. These results clearly demonstrate the power of MSAP technique for large-scale DNA methylation detection in rapeseed genome, and the complexity of DNA methylationchange during seed germination. DNA Hypomethyla- tion going with seed germination appears to be a necessary step toward transcriptional activation in gene expression, and may well contribute to the de- velopmental gene regulation. | LU Guangyuan WU Xiaoming CHEN Biyun GAO Guizhen XU Kun LI Xiangzhi | 2006 | Chinese Science Bulletin2006，51，2： | 14 |
| 5 | Comparisons in subcellular and biochemical behaviors of cadmium between Iow-Cd and high-Cd accumulation cultivars of pakchoi (Brassica chinensis L.)显示文摘 | Meng XUE Yihui ZHOU Zhongyi YANG Biyun LIN Jiangang YUAN Shanshan WU | 2014 | Frontiers of Environmental Science & Engineering2014，8，2： | 14 |
| 6 | Evaluation of Genetic and Epigenetic Modification in Rapeseed(Brassica napus)Induced by Salt Stress显示文摘咸度是为世界范围的油菜籽生产的一个重要限制环境因素。在这研究,我们估计了盐应力在油菜籽植物引起的脱氧核糖核酸损坏的程度和模式。放大碎片长度多型性(AFLP ) 分析表明在小植物的顺序改变的剂量相关的增加暴露了 000 mmol/L 氯化钠到 10-1。另外,暴露于一样的盐集中的单个小植物证明不同 AFLP 和选择区域放大了多型性 banding 模式。这些观察建议响应盐应力的那个脱氧核糖核酸变化在染色体是随机的,效果是剂量家属。响应应力也是的盐的脱氧核糖核酸甲基化变化由甲基化评估了敏感放大多型性(MSAP ) 。MSAP 乐队的三种类型被恢复。类型我乐队们与两 isoschizomers Hpa II 和 Msp 被观察我当类型 II 和类型 III 乐队仅仅与 Hpa II 和 Msp 被观察时我分别地。在在 NaCl 处理以后的 MSAP 乐队的类型的广泛的变化被观察,包括在也之间我, II 和 III 乐队,以及交换打的类型的外观和消失我和类型 II 或类型我和类型 III 乐队。0.2-17.6% cytosine methylated CCGG 地点的增加在与控制相比暴露于 10-200 mmol/L 盐的小植物被检测,并且这些变化包括了重新甲基化和 demethylation 事件。相关碎片也是的九甲基化恢复了并且定序,并且与乙烯分享高顺序相同的应答的元素绑定因素被识别。这些结果在对盐的回答强调,并且这些变化可以在盐应力下面为植物改编建议机制的小植物表明了基因的清楚的脱氧核糖核酸和 epigenetic 改变。 | Guangyuan Lu Xiaoming Wu Biyun Chen Guizhen Gao Kun Xu | 2007 | Journal of Integrative Plant Biology2007，49，11： | 14 |
| 7 | Increasing targeting scope of adenosine base editors in mouse and rat embryos through fusion of TadA deaminase with Cas9 variants显示文摘 | Lei Yang Xiaohui Zhang Liren Wang Shuming Yin Biyun Zhu Ling Xie Qiuhui Duan Huiqiong Hu Rui Zheng Yu Wei Liangyue Peng Honghui Han Jiqin Zhang Wenjuan Qiu Hongquan Geng Stefan Siwko Xueli Zhang Mingyao Liu Dali Li | 2018 | Protein & Cell2018，9，9： | 13 |
| 8 | Promoter methylation of Wnt/β-Catenin signal inhibitor TMEM88 is associated with unfavorable prognosis of nonsmall cell lung cancer显示文摘Objective:Recent research has indicated that altered promoter methylation of oncogenes and tumor suppressor genes is an important mechanism in lung cancer development and progression.In this study,we investigated the association between promoter methylation of TMEM88,a possible inhibitor of the Wnt/β-Catenin signaling,and the survival of patients with nonsmall cell lung cancer(NSCLC).Methods:Twelve pairs of tumor and adjacent non-tumor samples were used for microarray analyses of DNA methylation and gene expression.For validation,more than two hundred additional samples were analyzed for methylation using bisulfite pyrosequencing and for gene expression using q RT-PCR.Then the cell function were tested by wound healing,transwell,CCK8 and cell cycle assay.Results:Our analysis of patient specimens showed that TMEM88 methylation was higher in NSCLC tumors(82.2%±10.3,P<0.01)compared with the adjacent normal tissues(65.9%±7.2).The survival analysis revealed that patients with high TMEM88methylation had a shorter overall survival(46 months)compared with patients with low TMEM88 methylation(>56 months;P=0.021).In addition,we found that demethylation treatment could inhibit tumor cell proliferation,migration,and invasion,which was supportive of an association between methylation and survival.Conclusions:Based on these consistent observations,we concluded that TMEM88 may play an important role in NSCLC progression and that promoter methylation of TMEM88 may serve as a biomarker for NSCLC prognosis and treatment. | Rongna Ma Nannan Feng Xiao Yu Hongyan Lin Xiaohong Zhang Oumin Shi Huan Zhang Shuo Zhang Lei Li Min Zheng Ming Gao Herbert Yu Biyun Qian | 2017 | Cancer Biology & Medicine2017，14，4： | 6 |
| 9 | Evaluating ischemic stroke with diffusion tensor imaging显示文摘Objective:In recent years,diffusion tensor imaging (DTI) has emerged as a promising tool to study ischemic stroke. The aim of this study is to evaluate the anisotropic changes of cerebral white matter tracks in patients with ischemic stroke using DTI,and investigate the correlation between corticospinal tract damage and muscle strength in such patients during acute ischemia.Method:9 patients with acute ischemic stroke and 9 healthy subjects were examined with T1 weighted magnetic resonance imaging (MRI),T2 weighted MRI and DTI. Fractional anisotropy (FA) was measured and the three-dimensional fibrous band images of bilateral corticospinal tracts were reconstructed. The muscle strength of the affected hand was assessed by Brunnstorm standard[1],which is an internationally recognized measure of movement in hemiplegic patients.Results:①In the control group,there was no significant difference in FA between the bilateral corticospinal tracts,but FA in different white matter structure of the same side was significantly different (t=3.12,P < 0.05). FA of posterior limb of internal capsule was higher than that of knee and anterior limb of internal capsule,FA of anterior limb of internal capsule was the lowest. while in the patient group,FA of the infarcted sites was significantly lower than the contralateral ones (t=5.570,P < 0.01). ②The ipsilateral corticospinal tract demonstrated continuous interruption and the loss of consistent anatomic structure. The involved severity of corticospinal tract had significant correlation with that of muscle strength of the ipsilateral hand (r=0.83,P < 0.01).Conclusion:This study shows that DTI can be used to investigate ischemic stroke and assess ischemic stroke-induced damage. The damaged severity of corticospinal tracts is correlated with that of muscle strength. | Ziqian Chen Ping Ni Youqiang Ye Hui Xiao Gennian Qian Shangwen Xu Jingliang Wang Xizhang Yang Jinhua Chen Zhenshan Shi Biyun Zhang | 2008 | 福州总医院学报2008，15，3： | 6 |
| 10 | Knockdown of long non-coding RNA LCPAT1 inhibits autophagy in lung cancer显示文摘Objective: Long non-coding RNAs(lnc RNAs) are involved in numerous biological processes in lung cancer cells. In our previous studies, we identified a lnc RNA, ENST00000439577, which is highly expressed in lung carcinomas, and termed it lung cancer progression-associated transcript 1(LCPAT1). To characterize the role of LCPAT1 in lung cancer, we conducted the current study.Methods: Expression of LCPAT1 and autophagy-associated markers in tumor tissues and lung cancer cell lines was determined by real-time quantitative polymerase chain reaction(q PCR). Hematoxylin and eosin(HE) staining, q PCR, Western blot, and immunohistochemistry were performed to evaluate xenografted tumor tissues. Autophagy induced by rapamycin was detected by Western blot and immunofluorescence in lung cancer cell lines.Results: Expression of LCPAT1 and microtubule-associated protein 1 light chain 3 beta(LC3B) was positively correlated in lung cancer. Knockdown of LCPAT1 inhibited tumor growth and suppressed cell autophagy in vivo. Moreover, LCPAT1 knockdown in lung cancer cell lines resulted in decreased autophagy-associated gene expression and alleviated the cell autophagy induced by rapamycin.Conclusions: We speculate that LCPAT1 plays a crucial role in regulating autophagy in lung cancer. | Xiao Yu Xiaofei Ye Hongyan Lin Nannan Feng Sumeng Gao Xiaohong Zhang Yu Wang Herbert Yu Xiaobei Deng Biyun Qian | 2018 | Cancer Biology & Medicine2018，15，3： | 5 |
| 11 | Active surveillance as a management strategy for papillary thyroid microcarcinoma显示文摘Active surveillance(AS)can be considered as a treatment strategy for low risk papillary thyroid microcarcinoma(PTMC),with the absence of clinically apparent lymph nodes,extrathyroidal extensions,and distant metastasis.After reviewing the reports on AS of low risk PTMCs worldwide,we introduced AS,and discussed the selection criteria for active surveillance candidates based on different guidelines and the follow-up schedules.Moreover,the requirement of cytological diagnosis,progression evaluation methods,necessity of thyrotropin suppression,and medical costs were issues that both clinicians and patients considered.The usefulness of AS for low risk PTMC patients depended on accurate and confidential evaluation of patient risk.Clinicians may adopt measures like dynamic monitoring,risk stratification,and making personal follow-up schedules to minimize these potential risks.By appropriately selecting PTMC patients,AS can be an effective alternative treatment to immediate surgery. | Huan Zhang Xiangqian Zheng Juntian Liu Ming Gao Biyun Qian | 2020 | Cancer Biology & Medicine2020，17，3： | 5 |
| 12 | BIX-01294-enhanced chemosensitivity in nasopharyngeal carcinoma depends on autophagy-induced pyroptosis显示文摘Nasopharyngeal carcinoma(NPC)is a common cancer in southern China and Southeast Asia.Nowadays,radiotherapy is the therapy of choice for NPC patients,and chemotherapy has been found as an alternative treatment for advanced NPC patients.However,finding novel drugs and pharmacologically therapeutic targets for NPC patients is still urgent and beneficial.Our study showed that BIX-01294(BIX)can induce autophagic vacuoles formation and conversion of LC3B-I to LC3B-II in NPC cells in both dose-and time-dependent manners.Notably,the combination of BIX and chemotherapeutic drugs significantly decreased the cell viability and increased the lactate dehydrogenase release.Meanwhile,BIX plus cis-platinum(Cis)treatment induced pyroptosis in NPC cells as featured by cell swelling and bubble blowing from the plasma membrane,the increased frequency of annexin V and propidium iodide(PI)double-positive cells,as well as the cleavage of gasdermin E(GSDME)and caspase-3.Moreover,the deficiency of GSDME completely shifted pyroptosis to apoptosis.Furthermore,the inhibition of autophagy by chloroquine and the knockout of ATG5 gene significantly blocked the BIX-induced autophagy as well as pyroptosis in both in vitro and in vivo studies.Our data demonstrated that BIX-combined chemotherapeutic drugs could induce the Bax/caspase-3/GSDME-mediated pyroptosis through the activation of autophagy to enhance the chemosensitivity in NPC. | Qian Li Min Wang Yan Zhang Liuqian Wang Wei Yu Xiaomin Bao Biyun Zhang Yanghong Xiang Anchun Deng | 2020 | Acta Biochimica et Biophysica Sinica2020，52，10： | 5 |
| 13 | Characteristic analysis of phospholipid fatty acids (PLFAs) in typical nutrient polluted lake sediment in Wuhan显示文摘The process of urbanization aggravates the endogenous pollution of urban lake sediment,and polluted sediment may seriously affect the quality of the water in lakes.At present,it is difficult to distinguish the difference between sediment that is heavily polluted by nitrogen(N)and phosphorus(P)when using an analysis based on a physicochemical index classification.The current study applied phospholipid fatty acid(PLFA)technology to further analyze the sediment characteristics from the perspective of microbiology.Surface sediment from five urban lakes that are heavily polluted with N and P in Wuhan were sampled.Statistical approaches were used to analyze the microbial community structure in the sampled sediment,and to determine the correlations between the microorganisms and physicochemical indices.The most severely polluted lake sediment had similar PLFA structures,the highest saturated fatty-acid content,and bacteria as the dominant microorganism.However,there were differences between the microbial biomass of the various sediment samples,which may have been related to the degree of N and P pollution.Analysis of the microbial diversity in the sediment samples indicated that the bacteria were experiencing starvation and nutrient pressure,which may have been due to the dissolved oxygen concentration of the heavily polluted lake sediment.A correlation analysis showed that the endogenous N and P had different effects on the microbes of the polluted sediment.A redundancy analysis(RDA)demonstrated that the N/P ratio had the greatest influence on the PLFA species,accounting for 83%of the cumulative interpretation.To effectively promote the role of sediment microorganisms on circulating elements,it is necessary to regulate the N/P ratio of the sediment to some extent.When the N/P ratio in sediment exceeds 6,N pollution should be prioritized. | Xia Zhang Qianru Chen Chuan Wang Hongpei Zhang Yuqing Zhao Liping Zhang Biyun Liu Zhenbin Wu Qiaohong Zhou | 2021 | International Journal of Sediment Research2021，36，2： | 5 |
| 14 | Characterization of Humic Fractions in a ^(15)N-labelled Soil by Solid-State ^(13)C and ^(15)N NMR显示文摘Five humic fractions were obtained from a uniformly 15N-labelled soil by extraction with 0.1 mol L-1 Na4P207, 0.1 mol L-1 NaOH, and HF/HCI-0.1 mol L-1 NaOH, consecutively, and analyzed by 13C and 15N CPMAS NMR (cross polarization and magic angle spinning nuclear magnetic resonance). Compared with those of native soils humic fractions studied as a whole contained more alkyls, methoxyls and O-alkyls, being 27%-36%, 17%-21% and 36%-40%, respectively, but fewer aromatics and carboxyls (being 14%-20% and 13%-90%, respectively). Among those humic fractions, the humic acid (HA) and fulvic acid (FA) extracted by 0.1 mol L-1 Na4P207 contained slightly more carboxyls than corresponding humic fractions extracted by 0.1 mol L-1 NaOH, and the HA extracted by 0.1 mol L-1 NaOH after treatment with HF/HCI contained the least aromatics and carboxyls. The distribution of nitrogen functional groups of soil humic fractions studied was quite similar to each other and also quite similar to that of humic fraction from native soils. More than 75% of total N in each fraction was in amide form, with 9%-13% present as aromatic and/or aliphatic amines and the remainder as hoterocyclic N. | CHENG LILI, WEN QIXIAO and CHEN BIYUN Institute of Soil Science, the Chinese Academy of Sciences, Nanjing 210008 (China) | 2001 | Pedosphere2001，11，2： | 4 |
| 15 | Research on the degradation mechanism of pyridine in drinking water by dielectric barrier discharge显示文摘Pyridine, an important chemical raw material, is widely used in industry, for example in textiles,leather, printing, dyeing, etc. In this research, a dielectric barrier discharge(DBD) system was developed to remove pyridine, as a representative type of nitrogen heterocyclic compound in drinking water. First, the influence of the active species inhibitors tertiary butanol alcohol(TBA),HCO_3^-, and CO_3^(2-)on the degradation rate of pyridine was investigated to verify the existence of active species produced by the strong ionization discharge in the system. The intermediate and final products generated in the degradation process of pyridine were confirmed and analyzed through a series of analytical techniques, including liquid chromatography–mass spectrometry(LC–MS), high performance liquid chromatography(HPLC), ion chromatography(IC), total organic carbon(TOC) analysis, ultraviolet(UV) spectroscopy, etc. The results showed that the degradation of pyridine was mainly due to the strong oxidizing power of ozone and hydroxyl radical produced by the DBD system. Several intermediate products including 3-hydroxyl pyridine, fumaric acid, 2, 3-dihydroxypyridine, and oxalic acid were detected. Nitrogen was removed from the pyridine molecule to form nitrate. Through analysis of the degradation mechanism of pyridine, the oxidation pathway was deduced. The study provided a theoretical and experimental basis for the application of DBD strong ionization discharge in treatment of nitrogen heterocyclic compounds in drinking water. | Yang Li Rongjie Yi Chengwu Yi Biyun Zhou Huijuan Wang | 2017 | Journal of Environmental Sciences2017，29，3： | 4 |
| 16 | MRI and diffusion tensor imaging in assessing correlation of activation of cortical motor function and manifestations of corticospinal tract with muscle strength in patients with ischemic stroke显示文摘BACKGROUND: Ischemic stroke is often followed by the abnormalities of neurons and corticospinal tract, which can lead to corresponding clinical symptoms and signs. Recently, with the continuous perfection of high-field MRI instrument, it becomes possible to assess and investigate the cortical function and structural reconstruction following stroke by using MRI and diffusion tensor imaging (DTI). OBJECTIVE: To observe the cortical motor function and changes of corticospinal tracts by using MRI and DTI in the patients with ischemic stroke at acute period, compare with the normal subjects, and assess the damage of corticospinal tract and muscle strength. DESIGN: A case-control observation. SETTING: Department of Medical Imaging, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA. PARTICIPANTS: Nine inpatients (5 males and 4 females) with injury of motor function induced by acute ischemic stroke were selected from Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA between August and December in 2005, they aged 16-87 years with an average of 51 years old, and those with obvious conscious disturbances and severe cognitive disorders were excluded. At the same time, nine healthy right-handed physical examinees matched by age and gender with the patients were also selected, and they all had no nervous disease, epilepsy, mental diseases, cerebrovascular abnormalities and injury history, etc. All the subjects were informed with the detected items and agreed to participate in. METHODS: All the 9 patients with ischemic stroke at acute period and 9 healthy subjects were examined with MRI and DTI. ① A block-based design was used in the MRI, the passive finger-to-finger exercise was used as the stimulative task, and the static condition was taken as the baseline task. The GE 1.5T MRI system was used, all the data were processed after off-line, and analyzed with the SPM2 software, the association between the activated area and local anatomy of brain was observed. ② The DTI image was processed with the dTV.II.R1 software part of the anisotropic image and color anisotropic image were shown, and the three-dimensional white matter image of bilateral corticospinal tracts of the patients were reconstructed.③ The muscle strength of the affected hand of the stroke patients was judged with the Brunnstorm standard. MAIN OUTCOME MEASURES: The manifestations of MRI, DTI and corticospinal tracts of the 9 stroke patients were mainly observed. RESULTS: All the 9 stroke patients and 9 healthy subjects were involved in the analysis of results. ① Results of MRI examination: The handed exercise of the healthy subjects mainly activated contralateral sensorimotor cortex, posterior parietal cortex and bilateral supplementary motor areas. For the exercise of ipsilateral hand of 7 stroke patients, contralateral sensorimotor cortex was weakly activated, but ipsilateral posterial parietal cortex was obviously activated, and the activation of cerebrum was observed in 3 of them. For the other two patients, bilateral sensorimotor cortexes were activated during the exercise of ipsilateral hand, and the ipsilateral activated area was greater than the controlateral one. The exercise of contralateral hand activated contralateral sensorimotor cortex, posterior parietal cortex and contralateral supplementary motor area, and no obvious ipsilateral activated area was observed. ② Results of DTI examination: The anisotropic values of the infarcted area were obviously lower than those of the contralateral part (t=4.570, P < 0.01). the anisotropic value of the infarcted area were obviously was obviously correlated with bilateral muscle strength (t=0.888, P < 0.05). ③ Results of the examination for corticospinal tracts: The reconstruction of bilateral corticospinal tracts, contralateral anatomic form were generally identical with those of normal subjects, the consistency of each continuous form from anterior central gyrus downward to internal capsule to bridge of varolius and bulbus medullae. Because of the involvement of infarcted area to different extent, ipsilateral corticospinal tract was manifested by the interruption of succession and loss of consistency of anatomic structural form. The severity for the injury of corticospinal tract was markedly correlated with the muscle strength of the ipsilateral hand (r=0.888, P < 0.05). CONCLUSION: ① The MRI manifestations of stroke patients during the exercise of affected hand are different from those of normal subjects, suggesting that there is compensation and reconstruction of brain function. ② DTI examination is good for the judgement of the correlation between the damaged severity of infarction and muscle strength. ③ The damaged severity of corticospinal tracts is correlated with muscle strength, and it can be used to judge the prognosis of rehabilitative treatment. | Ziqian Chen Hui Xiao Biyun Zhang Gennian Qian Ping Ni Xizhang Yang | 2006 | Neural Regeneration Research2006，1，1： | 4 |
| 17 | Structural and metabolic responses of microbial community to sewage-borne chlorpyrifos in constructed wetlands显示文摘Long-term use of chlorpyrifos poses a potential threat to the environment that cannot be ignored, yet little is known about the succession of substrate microbial communities in constructed wetlands(CWs) under chlorpyrifos stress. Six pilot-scale CW systems receiving artificial wastewater containing 1 mg/L chlorpyrifos were established to investigate the effects of chlorpyrifos and wetland vegetation on the microbial metabolism pattern of carbon sources and community structure, using BIOLOG and denaturing gradient gel electrophoresis(DGGE) approaches. Based on our samples, BIOLOG showed that Shannon diversity(HV) and richness(S) values distinctly increased after 30 days when chlorpyrifos was added. At the same time, differences between the vegetated and the non-vegetated systems disappeared. DGGE profiles indicated that H Vand S had no significant differences among four different treatments. The effect of chlorpyrifos on the microbial community was mainly reflected at the physiological level. Principal component analysis(PCA) of both BIOLOG and DGGE showed that added chlorpyrifos made a difference on test results.Meanwhile, there was no difference between the vegetation and no-vegetation treatments after addition of chlorpyrifos at the physiological level. Moreover, the vegetation had no significant effect on the microbial community at the genetic level. Comparisons were made between bacteria in this experiment and other known chlorpyrifos-degrading bacteria. The potential chlorpyrifos-degrading ability of bacteria in situ may be considerable. | Dan Zhang Chuan Wang Liping Zhang Dong Xu Biyun Liu Qiaohong Zhou Zhenbin Wu | 2016 | Journal of Environmental Sciences2016，28，6： | 3 |
| 18 | Characters of MR diffusion tensor imaging in cerebral ischemic corticospinal tract injury显示文摘BACKGROUND: Diffusion tensor imaging (DTI) is one of the noninvasive methods to study the morphological structure of brain white matter fibrous bands in vivo, and it has been applied primarily in clinic. DTI is acknowledged as the more effective imaging method to diagnose ultra-acute and/or acute cerebral infarction. OBJECTIVE: To observe the anisotropic characters of cerebral white matter fibrous bands in patients with ischemic stroke by using DTI, and investigate the correlation between the damage of corticospinal tract and muscle strength in patients with ischemic stroke at acute period. DESIGN: A case-control observation. SETTING: Department of Medical Imaging, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA. PARTICIPANTS: Nine inpatients with injury of motor function induced by acute ischemic stroke (patient group) at 6 hours to 2 weeks after the attack were selected from the Department of Neurology, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA from September 2005 to March 2006, and they all accorded with the present diagnostic standard for cerebrovascular disease in China. There were 5 males and 4 females, aged 16-87 years. At the same time, nine healthy right-handed physical examinees matched by age and sex with the patients were taken as the control group, and they all had no nervous disease, mental diseases, cerebrovascular abnormalities and injury history, etc. All the subjects were informed with the detected items and agreed to participate in the study. METHODS: All the 9 patients with ischemic stroke at acute period and 9 healthy subjects were examined with MRI, T1 weighted imaging, T2 weighted imaging and DTI. And the data were processed offline with dTV.II software, the images of fractional anisotropy and directional encoded color (DEC) were obtained, and the three-dimensional fibrous band images of bilateral corticospinal tracts were reconstructed. In the control group, the values of fractional anisotropy of main white matter fibrous bands were measured in the region of interest (ROI) of the anterior limb, knee and posterior limb of internal capsule. In the patient group, the values of fractional anisotropy of white matter were measured in the infarcted sites and corresponding contralateral sites of the patients. The ROI was set in bilateral cerebral peduncles to reconstruct three-dimensionally the bilateral corticospinal tracts. The muscle strength of the affected hand was assessed with Brunnstorm standard in the stroke patients. MAIN OUTCOME MEASURES: The characters of DTI and images of the value of fractional anisotropy, and the manifestations of three-dimensional corticospinal tracts were observed in the two groups. RESULTS: All the data from the 9 patients and 9 healthy volunteers were involved in the analysis of results. ① In the control group, the white matter and gray matter could be distinguished clearly in the image of fractional anisotropic values, the fibers of different directions were shown by different colors in DEC picture, which clearly demonstrated the normal anatomic structure and direction of white matter fibers. In the patient group, the infarctions occurred in the gray matter or white matter could be distinguished in the images of fractional anisotropic values, DEC picture could clearly show the direct influence of the infarcted site on the white matter fibers. The fractional anisotropic values in different white matter structure of the same side were significantly different in the control group (t=3.12, P < 0.05), and the reconstructed images fractional anisotropic values and DEC picture could show most of the main white matter fibrous bands. The fractional anisotropic values of the infarcted sites were significantly lower than the contralateral ones in the patient group (t=5.570, P < 0.01). ② The reconstructed bilateral corticospinal tracts showed that the anatomic forms of the contralateral corticospinal tract of the patients were almost identical to those of normal people, it started from precentral gyrus, downward to the nternal capsule, and extended to pontine and medulla oblongata, each fibrous band was continuous, and the form had good consistency. Because of the involvement of infarction of different severity, the ipsilateral corticospinal tract manifested as continuous interruption and the loss of consistent anatomic structural form. The involved severity of corticospinal tract had significant correlation with that of muscle strength of the ipsilateral hand (r=1.30, P < 0.01). CONCLUSION: ① DTI can display the direction and distribution of cerebral white matter fibrous bands. ② DTI images of fractional anisotropic values and DEC can show the directions and anisotropic degree of white matter fibers in the infarcted sites of stroke patients. ③ The three-dimensional images of fibrous bands can show the conditions of pyramidal tracts more directly. ④ The damaged severity of corticospinal tracts is correlated with that of muscle strength. | Ziqian Chen Ping Ni Hui Xiao Youqiang Ye Gennian Qian Shangwen Xu Xizhang Yang Jinhua Chen Biyun zhang | 2006 | Neural Regeneration Research2006，1，2： | 3 |
| 19 | Threonine 32 (Thr32) of FoxO3 is critical for TGF-β-induced apoptosis via Bim in hepatocarcinoma cells显示文摘转变生长因素 --(TGF-) 在恶意的细胞的各种各样的类型上施加 apoptotic 效果,包括肝癌症细胞。然而, TGF- 由导致 apoptosis 的精确机制仍然保持糟糕已知。在现在的学习,我们显示出那 threonine 32 (Thr32 ) FoxO3 的残余是批评的让 TGF- 在 hepatocarcinoma Hep3B 房间经由 Bim 导致 apoptosis。我们的数据证明 TGF- 在 Thr32 通过特定的 de-phosphorylation 导致了 FoxO3 激活。TGF -- 激活的 FoxO3 与 Smad2/3 合作了调停 Bim 起来规定和 apoptosis。在 Thr32 的 FoxO3 (de ) phosphorylation 被酷蛋白 kinase 调整我 --(CKI-) 。由小分子 D4476 的 CKI 抑制能废除 TGF -- 导致的 FoxO/Smad 激活,反向的 Bim 起来规定,和块顺序的 apoptosis。更重要地, CKI- 和 p32FoxO3 的 deregulated 层次在人的恶意的肝纸巾被发现。一起拿,我们的调查结果建议可能有 FoxO3 的 Thr32 为 TGF 是枢轴的一台 CKI-FoxO/Smad-Bim 在引擎 -- 导致的 apoptosis,为肝癌症治疗使它成为一个潜在的治疗学的目标。 | Xiangxuan Zhao Yong Liu Lei Du Leya He Biyun Ni Junbo Hu Dahai Zhu Quan Chen | 2015 | Protein & Cell2015，6，2： | 3 |
| 20 | Influence of extreme weather and meteorological anomalies on outbreaks of influenza A (H1N1)显示文摘Biological experiments and epidemiological evidence indicate that variations in environment have important effect on the occurrence and transmission of epidemic influenza.It is therefore important to understand the characteristic patterns of transmission for prevention of disease and reduction of disease burden.Based on case records,we analyzed the environmental characteristics including climate variables in Changsha,and then constructed a meteorological anomaly susceptive-infective-removal (SIR) model on the basis of the results of influenza A (H1N1) transmission.The results showed that the outbreak of influenza A (H1N1) in Changsha showed significant correlation with meteorological conditions;the spread of influenza was sensitive to meteorological anomalies,and that the outbreak of influenza A (H1N1) in Changsha was influenced by a combination of absolute humidity anomalous weather conditions,contact rates of the influenza patients and changes in population movements.These findings will provide helpful information regarding prevention strategies under different conditions,a fresh understanding of the emergence and re-emergence of influenza outbreaks,and a new perspective on the transmission dynamics of influenza. | XIAO Hong TIAN HuaiYu LIN XiaoLing GAO LiDong DAI XiangYu ZHANG XiXing CHEN BiYun ZHAO Jian XU JingZhe | 2013 | Science China(Technological Sciences)2013，56，3： | 2 |
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