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| 1 | Synthesis and Application of Novel Triazine-Based Charring-Foaming Agents in Intumescent Flame Retardant Polypropylene显示文摘Three novel triazine-based charring-foaming agents (M2-CFA,M4-CFA,and M6-CFA) were synthesized successfully with only water as the solvent and were characterized by FT-IR and thermal gravimetric analysis (TGA).These three charring-foaming agents and the byproduct 944-by were employed to study the effectiveness of the novel intumescent flame retardant dopant on the fire retardancy of polypropylene (PP) investigated through UL-94 and limiting oxygen index (LOI) tests.TGA results showed that the M4-CFA presented good char formation ability (char residue: 26.8% at 700 ℃).It was found that the sample with a 2/1 mass ratio of APP to M4-CFA exhibited the best flame retardancy among all the PP composites: 35.5% LOI and a V-0 rating of UL-94.Additionally,the microstructure and morphology of char residues were further studied by XRD,Raman spectroscopy and SEM. | Ligong Chen Yanyan Yuan Bowei Wang Na Liu Yuzhi Xing Yang Li | 2017 | Transactions of Tianjin University2017，23，3： | 9 |
| 2 | Zacopride selectively activates the Kir2.1 channel via a PKA signaling pathway in rat cardiomyocytes显示文摘We recently reported that zacopride is a selective inward rectifier potassium current (IK1 ) channel agonist, suppressing ventricular arrhythmias without affecting atrial arrhythmias. The present study aimed to investigate the unique pharmacological properties of zacopride. The whole-cell patch-clamp technique was used to study IK1 currents in rat atrial myocytes and Kir2.x currents in human embryonic kidney (HEK)-293 cells transfected with inward rectifier potassium channel (Kir)2.1, Kir2.2, Kir2.3, or mutated Kir2.1 (at phosphorylation site S425L). Western immunoblots were performed to estimate the relative protein expression levels of Kir2.x in rat atria and ventricles. Results showed that zacopride did not affect the IK1 and transmembrane potential of atrial myocytes. In HEK293 cells, zacopride increased Kir2.1 homomeric channels by 40.7%±9.7% at 50 mV, but did not affect Kir2.2 and Kir2.3 homomeric channels, and Kir2.1-Kir2.2, Kir2.1-Kir2.3 and Kir2.2-Kir2.3 heteromeric channels. Western immunoblots showed that similar levels of Kir2.3 protein were expressed in rat atria and ventricles, but atrial Kir2.1 protein level was only 25% of that measured in the ventricle. In addition, 5-hydroxytryptamine (5-HT) 3 receptor was undetectable, whereas 5-HT 4 receptor was weakly expressed in HEK293 cells. The Kir2.1-activating effect of zacopride in these cells was abolished by inhibition of protein kinase A (PKA), but not PKC or PKG. Furthermore, zacopride did not activate the mutant Kir2.1 channel in HEK293 cells but selectively activated the Kir2.1 homomeric channel via a PKA-dependent pathway, independent to that of the 5-HT receptor. | ZHANG Li LIU QingHua LIU ChengFang ZHAI XuWen FENG QiLong XU RuiLing CUI XiangLi ZHAO ZhiQing CAO JiMin WU BoWei | 2013 | Science China(Life Sciences)2013，56，9： | 7 |
| 3 | Insight into the topology effect on the diffusion of ethene and propene in zeolites: A molecular dynamics simulation study显示文摘Selectivity control is a difficult scientific and industrial challenge in methanol-to-olefins(MTO)conversion.It has been experimentally established that the topology of zeolite catalysts influenced the distribution of products.Besides the topology effect on reaction kinetics,the topology influences the diffusion of reactants and products in catalysts as well.In this work,by using COMPASS force-field molecular dynamics method,we investigated the intracrystalline diffusion of ethene and propene in four different zeolites,CHA,MFI,BEA and FAU,at different temperatures.The self-diffusion coefficients and diffusion activation barriers were calculated.A strong restriction on the diffusion of propene in CHA was observed because the self-diffusion coefficient ratio of ethene to propene is larger than 18 and the diffusion activation barrier of propene is more than 20 kJ/mol in CHA.This ratio decreases with the increase of temperature in the four investigated zeolites.The shape selectivity on products from diffusion perspective can provide some implications on the understanding of the selectivity difference between HSAPO-34 and HZSM-5 catalysts for the MTO conversion. | Chuanming Wang Bowei Li Yangdong Wang Zaiku Xie | 2013 | Journal of Energy Chemistry2013，22，6： | 6 |
| 4 | A Multisource Contour Matching Method Considering the Similarity of Geometric Features显示文摘The existing multi-source contour matching studies have focused on the matching methods with consideration of topological relations and similarity measurement based on spatial Euclidean distance,while it is lack of taking the contour geometric features into account,which may lead to mismatching in map boundaries and areas with intensive contours or extreme terrain changes.In light of this,it is put forward that a matching strategy from coarse to precious based on the contour geometric features.The proposed matching strategy can be described as follows.Firstly,the point sequence is converted to feature sequence according to a feature descriptive function based on curvature and angle of normal vector.Then the level of similarity among multi-source contours is calculated by using the longest common subsequence solution.Accordingly,the identical contours could be matched based on the above calculated results.In the experiment for the proposed method,the reliability and efficiency of the matching method are verified using simulative datasets and real datasets respectively.It has been proved that the proposed contour matching strategy has a high matching precision and good applicability. | Wenyue GUO Anzhu YU Qun SUN Shaomei LI Qing XU Bowei WEN Yuanfu LI | 2020 | Journal of Geodesy and Geoinformation Science2020，3，3： | 5 |
| 5 | Anti-rutting performance evaluation of modified asphalt binders: A review显示文摘Asphalt pavement inevitably suffers rutting distress induced by repeated loads under high temperatures, which can be effectively alleviated by the modification of asphalt binders.This paper summarized the asphalt modifiers for enhancing the performance of asphalt pavement at high temperatures in terms of the modifier type, additive amount and improving effect. Then, the anti-rutting performance evaluation methods of asphalt binder were introduced with details, including the ring and ball(R&B) test, the dynamic viscosity test, the zero/low shear viscosity(ZSV/LSV) test, the dynamic shear rheometer(DSR) test,the repeated creep and recovery(RCR) test, and the multiple stress creep and recovery(MSCR) test. Furthermore, the sensitivity of the evaluation method to asphalt modifier was analyzed using the normalization method. It was found that the indicators from the MSCR test could clearly reveal the influence of asphalt modifiers on high temperature performance of asphalt binder. But it still had some limitations affecting the accuracy of evaluation results, such as the number of creep-recovery cycles, the stress level and the recovery time. In addition, according to the rutting resistance improvement ratio(RRIR),the styrene-butadiene-styrene(SBS) modifier performed better in improving the rutting resistance of asphalt binder, compared with other modifiers when added within the usual dosage range. | Yan Li Peiwen Hao Chaozhi Zhao Jwnqiang Ling Tao Wu Dewen Li Jingwen Liu Bowei Sun | 2021 | Journal of Traffic and Transportation Engineering(English Edition)2021，8，3： | 4 |
| 6 | Enhancing the mechanical and anticorrosion properties of 316L stainless steel via a cathodic plasma electrolytic nitriding treatment with added PEG显示文摘A cathodic plasma electrolytic nitriding(CPEN)treatment with a urea aqueous solution was performed on 316L stainless steel to rapidly improve its surface properties in this work.Test results show that the PEG2000 macromolecules increased the nitriding energy via enhancing the ability to bond the produced gas film to the metal/electrolyte interface.The cross-sectional morphologies indicate that a thick nitrided layer was obtained when the urea concentration was 543 g I^-1,corresponding to a Vickers hardness 450 HV(0.1),which was 3.5 times larger than that of the substrate.The nitrided layer mainly contained expanded austenite(γN),oxides and iron nitrides(e.g.,Fe3O4 and FeN(0.076)).In terms of its performance,coefficient of friction(COF)of the nitride layer decreased to nearly two-thirds that of the untreated layer,and the passivation current densities of the nitrided sample in a 3.5%NaCl solution decreased by an order of magnitude compared to that of the substrate.Therefore,the approach presented herein provides an attractive way to modify the effect of CPEN in a urea aqueous solution. | Tianyi Zhang Junsheng Wu Lei Jin Zhan Zhang Wan Rong Bowei Zhang Yi Wang Yedong He Wei Liu Xiaogang Li | 2019 | Journal of Materials Science & Technology2019，35，11： | 3 |
| 7 | EGFR signaling augments TLR4 cell surface expression and function in macrophages via regulation of Rab5a activation显示文摘Dear Editor,oll-like receptor 4(TLR4)is a key receptor sensing bacterial lipopolysaccharide(LPS),and is the most investigated member of the Tol-like receptor family(Kawai and Akira,2007;Kayagaki et al,2013;Klein et al.,2015).Cell surface TLR4 expression is determined by the balance between receptor trafficking from the Golgi apparatus to the cell membrane,and internalization of the cell surface receptor into endosomal compartments(Saltoh,2009).In bone mar-row-derived macrophages(BMDM),we observed LPS-in-duced EGFR phosphorylation on the surface of BMDM,and this was inhibited by pretreatment with PD168393 or TAPI-1(Fig.S1).Next,we measured dynamic changes in cell sur-face TLR4 expression after LPS treatment.At6,12,and 24 h after LPS treatment,TLR4 expression on the surface of BMDM was increased^2-,~6-,and^9-fold,respectively,as compared with controls. | Jing Tang Bowei Zhou Melanie JScott Linsong Chen Dengming Lai Erica KFan Yuehua Li Qiang Wu Timothy RBilliar Mark AWilson Ping Wang Jie Fan | 2020 | Protein & Cell2020，11，2： | 2 |
| 8 | Novel Modular Multilevel Converter Against DC Faults for HVDC Applications显示文摘In view of the DC fault current isolation deficiency for the conventional half-bridge sub-module(HBSM)based modular multilevel converter(MMC),this paper presents an improved MMC topology.Both quasi reverse blocking submodules(QRBSMs)and current limit modules(CLMs)are employed to improve the DC fault handling capability for HVDC applications.This paper analyzes such a new converter configuration and operation principles.Then the DC pole-to-pole short circuit fault is taken into consideration for further study,as well as the fault current blocking mechanism and quantitative relationship between system electrical stress and key parameters.To validate the feasibility of the proposed topology and fault protection theory,extensive simulation results are demonstrated.It is concluded that the QRB-MMC can effectively block the fault current under DC fault condition.In addition,CLMs play an important role in further accelerating fault current attenuation.Moreover,QRB-MMC employs the original control and modulation strategies under normal operation conditions;thus,it further reduces the complexity of industry design. | Xiaofeng Yang Yao Xue Bowei Chen Zhiqin Lin Trillion Q.Zheng Yan Li | 2017 | CSEE Journal of Power and Energy Systems2017，3，2： | 2 |
| 9 | Preparation of Large Area Double Sided YBCO Thin Films by DC Magneton Sputtering显示文摘Tc, Jc and Rs properties of large area double sided YBCO thin films deposited on LaAlO3 substrates by direct current sputtering were reported.Film thickness of the obtained thin films is over 300 nm, Tc > 90 K; Rs can be as low as less than 1.0 mΩ(77 K, 10 GHz).Homogenousity of the properties around the plane was studied.Under the measnous distributions.Influences of substrate temperature and Ar and O2 pressures on properties of the double-sided thin films were discussed. | Li Tao Gu Hongwei Wang Peiwen Wang Xiaoping Xie Bowei | 2004 | Journal of Rare Earths2004，22，z1： | 2 |
| 10 | Cobalt tungsten phosphide with tunable W-doping as highly efficient electrocatalysts for hydrogen evolution reaction显示文摘It has been of interest in seeking electrocatalysts that could exercise equally high-efficient and durable hydrogen evolution upon nonselective electrolytes in both acidic and alkaline environments. Herein, we report a facile strategy to fabricate cobalt tungsten phosphides (CoxW2−xP2/C) hollow polyhedrons with tunable composition based on metal-organic frameworks (MOFs) template method. By the deliberate control of W doping, the synthesized catalyst with the composition of Co0.9W1.1P2/C is found to be able to achieve a current density of 10 mA·cm^(−2) at overpotentials of 35 and 54 mV in acidic and alkaline media, respectively. This combined electrochemical property stands atop the state-of-the-art electrocatalyst counterparts. To unveil the peculiar behavior of the structure, density functional theory (DFT) calculation was implemented and reveals that the surface W-doping facilitates the optimization of hydrogen absorption free energy (ΔGH*) as well as the thermodynamic and kinetics barriers for water dissociation, which is coupled with the hollow structure of Co-W phosphides, leading to the prominent HER catalytic performance. | Bowei Zhang Chaojiang Li Jun Hu Dongdong Peng Kang Huang Junsheng Wu Zhong Chen Yizhong Huang | 2021 | Nano Research2021，14，11： | 1 |
| 11 | Crude-oil hydrocarbon composition characteristics and oil viscosity prediction in the northern Songliao Basin显示文摘Crude oil hydrocarbon composition characteristics and oil viscosity prediction are important bases in petroleum exploration.A total of 54 oil/heavy-oil samples and 17 oil sands were analyzed and quantified using both comprehensive 2D gas chromatography(GC×GC)and comprehensive 2D gas chromatography/time-of-flight mass spectrometry(GC×GC/TOFMS).The results show that crude oil in the West slope is mainly heavy oil and its hydrocarbon composition is characterized overall by paraffins>mono-aromatics>naphthenes>non-hydrocarbons>di-aromatics>tri-aromatics>tetra-aromatics.Aromatics are most abundant and non-hydrocarbons are least abundant,whilst content differences among paraffins,naphthenes,aromatics,and non-hydrocarbons are less than 15%.There are two types of heavy oil,secondary type and mixing type.Biodegradation is the main formation mechanism of heavy oil.Biodegradation levels cover light biodegradation,moderate biodegradation,and severe biodegradation.With increasing biodegradation,paraffin content decreases while contents of aromatics and nonhydrocarbons increase.In contrast,naphthene content increases first and then decreases with increasing biodegradation.In severe biodegradation stage,naphthenes decrease more quickly than aromatics and non-hydrocarbons.This provides a new method for studying oil/heavy-oil biodegradation mechanism and biodegradation resistance of different hydrocarbons at different biodegradation stages.In the Longhupao-Daan terrace and Qijia-Gulong depression,most crude oil is conventional oil.Its composition is dominated by paraffins with the lowest content of aromatics.In some casual oil wells from the Longhupao-Daan terrace,crude oil from Saertu oil reservoirs is moderately biodegraded whereas crude oil from Putaohua oil reservoir is lightly biodegraded.Chemical parameters using saturate hydrocarbons and aromatics are usually not suitable for determining organic type and thermal maturity of biodegraded oil,especially of moderately or severely biodegraded oil,whilst Ts/(Ts+Tm)ratio can be used to determine thermal maturity of both conventional crude oil and heavy oil. | ZHANG JuHe FENG ZiHui FANG Wei HUO QiuLi ZHANG Kun LI JingKun ZENG HuaSen ZHANG BoWei | 2014 | Science China Earth Sciences2014，57，2： | 1 |
| 12 | AgPdCo hollow nanospheres electrocatalyst with high activity and stability toward the formate electrooxidation显示文摘The widespread commercial application of direct formate fuel cell(DFFC)is limited by the lack of efficient electrocatalysts for the formate oxidation reaction(FOR).AgPdCo hollow nanospheres(H-NSs)with jagged surfaces are successfully synthesized via a facile method involving the wet-chemical synthesis of AgPdCo nanospheres(NSs)and galvanic replacement reaction between Pd salt and AgPdCo NSs.Surpassing Ag_(30)Pd_(69)Co_(1) NSs and most of previously reported electrocatalysts,Ag_(9)Pd_(90)Co_(1) H-NSs exhibit extremely high FOR activity with a peak current density of 3.08 A·mg_(Pd)^(−1).Apart from the competitive activity,Ag_(9)Pd_(90)Co_(1) H-NSs show greatly improved chronoamperometric and cycling stability,whereby the current density retains about 0.24 A·mg_(Pd)^(−1) after 3,600 s electrocatalysis and the mass activity maintains 54.06%of the initial value after 500 cycles.The unique hollow nanosphere and synergistic effect are responsible for the enhanced activity and stability.This study will provide new clues for the development of outstanding electrocatalysts. | Qiao Wang Fuyi Chen Quan Tang Longfei Guo Tao Jin Bowei Pan Junpeng Wang Zhen Li Bo Kou Weiqi Bian | 2021 | Nano Research2021，14，7： | 1 |
| 13 | A failure criterion for shale considering the anisotropy and hydration based on the shear slide failure model显示文摘A failure criterion fully considering the anisotropy and hydration of shale is essential for shale formation stability evaluation.Thus,a novel failure criterion for hydration shale is developed by using Jaeger’s shear failure criterion to describe the anisotropy and using the shear strength reduction caused by clay minerals hydration to evaluate the hydration.This failure criterion is defined with four parameters in Jaeger’s shear failure criterion(S_(1),S_(2),a andφ),three hydration parameters(k,ω_(sh)andσ_(s))and two material size parameters(d and l0).The physical meanings and determining procedures of these parameters are described.The accuracy and applicability of this failure criterion are examined using the published experimental data,showing a cohesive agreement between the predicted values and the testing results,R^(2)=0.916 and AAREP(average absolute relative error percentage)of 9.260%.The error(|D_(p)|)is then discussed considering the effects ofβ(angle between bedding plane versus axial loading),moisture content and confining pressure,presenting that|Dp|increases whenβis closer to 30°,and|D_(p)|decreases with decreasing moisture content and with increasing confining pressure.Moreover,|D_(p)|is demonstrated as being sensitive to S1and being steady with decrease in the data set whenβis 0°,30°,45°and 90°. | Qiangui Zhang Bowei Yao Xiangyu Fan Yong Li Nicholas Fantuzzi Tianshou Ma Yufei Chen Feitao Zeng Xing Li Lizhi Wang | 2023 | International Journal of Mining Science and Technology2023，33，4： | 1 |
| 14 | Unravelling the effects of complexation of transition metal ions on the hydroxylation of catechol over the whole pH region显示文摘Catechol pollutants(CATPs)serving as chelating agents could coordinate with many metal ions to form various CATPs-metal complexes.Little information is available on the effects of complexation of metal ions on CATPs degradation.This work presents a systematical study of·OH-mediated degradation of catechol and catechol-metal complexes over the whole pH range in advanced oxidation processes(AOPs).Results show that the pH-dependent complexation of metal ions(Zn^(2+),Cu^(2+),Ti^(4+)and Fe^(3+))promotes the deprotonation of catechol under neutral and even acidic conditions.The radical adduct formation(RAF)reactions are both thermodynamically and kinetically favorable for all dissociation and complexa-tion species,and-OH/-O-group-containing C positions are more vulnerable to·OH attack.The kinetic results show that the complexation of the four metal ions offers a wide pH range of effectiveness for catechol degradation.At pH 7,the apparent rate constant(k_(app))values for different systems follow the order of catechol+Ti^(4+)≈catechol+Zn^(2+)>catechol+Cu^(2+)>catechol+Fe^(3+)>catechol.The mechanistic and kinetic results would greatly improve our understanding of the degradation of CATPs-metal and other organics-metal complexes in AOPs.The toxicity assessment indicates that the·OH-based AOPs have the ability for decreasing the toxicity and increasing the biodegradability during the processes of catechol degradation. | Zexiu An Jianfei Sun Qiong Mei BoWei Mingxue Li Ju Xie Maoxia He Qiao Wang | 2022 | Journal of Environmental Sciences2022，34，5： | 1 |
| 15 | Dynamic value iteration networks for the planning of rapidly changing UAV swarms显示文摘In an unmanned aerial vehicle ad-hoc network(UANET),sparse and rapidly mobile unmanned aerial vehicles(UAVs)/nodes can dynamically change the UANET topology.This may lead to UANET service performance issues.In this study,for planning rapidly changing UAV swarms,we propose a dynamic value iteration network(DVIN)model trained using the episodic Q-learning method with the connection information of UANETs to generate a state value spread function,which enables UAVs/nodes to adapt to novel physical locations.We then evaluate the performance of the DVIN model and compare it with the non-dominated sorting genetic algorithm II and the exhaustive method.Simulation results demonstrate that the proposed model significantly reduces the decisionmaking time for UAV/node path planning with a high average success rate. | Wei LI Bowei YANG Guanghua SONG Xiaohong JIANG | 2021 | Frontiers of Information Technology & Electronic Engineering2021，22，5： | 1 |
| 16 | Heterologous expres- sion of the TsVP gene improves the drought resis- tance of maize显示文摘 | Li BoWei A Y Song C X | 2008 | Plant Biotechnology Journal2008，6，： | 1 |
| 17 | A PTEN translational isoform has PTEN-like activity显示文摘Background:To identify PTEN isoform and explore its potential role in tumor suppression.Methods:Western blotting,over-expression,shRNA mediated knocking-down,and bioinformatic analysis were used to identify PTEN isoform and test its effect on PI3K-Akt signaling pathway.Cell proliferation,apoptosis,and migration assays were used to test PTEN isoform's biological activities.Results:The PTEN isoform is about 15 kDa bigger than PTEN and its expression is dependent on PTEN status.Immunoprecipitation for PTEN isoform followed by screening with antibodies against ISG15,SUMO1/2/3,Ubiquitin,and Nedd8 showed the identified PTEN isoform is not a general proteinaceous post-translational modification.In addition,overexpression of PTEN cDNA in cells did not generate PTEN isoform whereas knocking-down of PTEN reduced the protein levels of both PTEN and PTEN isoform in a proportional manner.Analysis of PTEN DNA sequence disclosed an alternative translational starting code(CTG) upstream of canonical PTEN coding sequence.Expression of cloned PTEN isoform generated a protein with a size about 15 kDa bigger than PTEN and suppressed PI3K-Akt signaling pathway in cells.Overexpression of PTEN isoform also led to decrease in cell growth and enhanced serum starvation—and UV irradiation—induced apoptosis through activation of Caspase 3.Finally,expression of PTEN isoform inhibited cell migration in scratch assay.Conclusions:PTEN isoform has PTEN-like activity and might be a new tumor suppressor. | Xie Zhang Bowei Yin Fangfang Zhu Guochang Huang Hong Li | 2015 | Chinese Journal of Cancer Research2015，27，5： | 1 |
| 18 | Towards prediction of ordered phases in rechargeable battery chemistry via group–subgroup transformation显示文摘The electrochemical thermodynamic and kinetic characteristics of rechargeable batteries are critically influenced by the ordering of mobile ions in electrodes or solid electrolytes.However,because of the experimental difficulty of capturing the lighter migration ion coupled with the theoretical limitation of searching for ordered phases in a constrained cell,predicting stable ordered phases involving cell transformations or at extremely dilute concentrations remains challenging.Here,a group-subgroup transformation method based on lattice transformation and Wyckoff-position splitting is employed to predict the ordered ground states.We reproduce the previously reported Li_(0.75)CoO_(2),Li_(0.8333)CoO_(2),and Li_(0.8571)CoO_(2)phases and report a new Li_(0.875)CoO_(2)ground state.Taking the advantage of Wyckoff-position splitting in reducing the number of configurations,we identify the stablest Li_(0.0625)C_(6) dilute phase in Li-ion intercalated graphite.We also resolve the Li/La/vacancy ordering in Li_(3x)La_(2/3−x)TiO_(3)(0 | Yunbing Ran Zheyi Zou Bo Liu Da Wang Bowei Pu Penghui Mi Wei Shi Yajie Li Bing He Ziheng Lu Xia Lu Baihai Li Siqi Shi | 2021 | npj Computational Materials2021，，1： | 1 |
| 19 | Author Correction: Towards prediction of ordered phases in rechargeable battery chemistry via group-subgroup transformation显示文摘The original version of this Article contained error in DATA AVAILABILITY,in which the website hyperlink is not valid and should be revised to https://github.com/shuhebing/gsop.The same error also occurs in CODE AVAILABILITY,in which the website hyperlink should also be revised to https://github.com/shuhebing/gsop. | Yunbing Ran Zheyi Zou Bo Liu Da Wang Bowei Pu Penghui Mi Wei Shi Yajie Li Bing He Ziheng Lu Xia Lu Baihai Li Siqi Shi | 2022 | npj Computational Materials2022，，1： | 0 |
| 20 | A novel polymer-based nitrocellulose platform for implementing a multiplexed microfluidic paperbased enzyme-linked immunosorbent assay显示文摘Nitrocellose(NC)membranes,as porous paper-like substrates with high protein-binding capabilties,are very popular in the field of point-of-care immunoassays.However,generating robust hydrophobic structures in NC membranes to fabricate microfluidic paper-based analytical devices(μPADs)remains a great challenge.At present,the main method relies on an expensive wax printer In addition,NC membranes very easy to adhere during the printing process due to electrostatic adsorption.Herein,we developed a facile,fast and low-cost strategy to fabricateμPADs in NC membranes by screen-printing polyurethane acrylate(PUA)as a barrier material for defning flow channels and reaction zones.Moreover,hydrophobic barriers based on UV-curable PUA can resist various surfactant solutions and organic solvents that are generally used in immunoassays and biochemical reactions.To validate the feasibility of this PUA-based NC membrane for immunoassays in point-of-care testing(POCT),we further designed and assembled a rotational paperbased analytical device for implementing a multiplexed enzyme-linked immunosorbent assay(ELISA)in a simple manner.Using the proposed device under the optimal conditions,alpha fetoprotein(AFP)and carcinoembryonic antigen(CEA)could be identified,with limits of detection of 136 pg/mL and 174 pg/mL,respectively,which are below the threshold values of these two cancer biomarkers for clinical diagnosis.We believe that this reliable device provides a promising plaform for the diagnosis of disease based on ELISA or other related bioassays in limited setings or remote regions. | Dong Lin Bowei Li Longwen Fu Ji Qi Chunlei Xia Yi Zhang Jiadong Chen Jaebum Choo Lingxin Chen | 2022 | Microsystems & Nanoengineering2022，8，3： | 0 |
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