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| 1 | A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants显示文摘CRISPR/Cas9 染色体指向系统被用于许多种类。然而,为植物报导的 mostCRISPR/Cas9 系统能仅仅修改一个或一些指向地点。这里,我们报导一个柔韧的 CRISPR/Cas9 向量系统,利用植物 codon 优化了 Cas9 基因,为方便、高效率的复合染色体在 monocot 和 dicot 植物编辑。我们设计了基于 PCR 的过程很快产生多重 sgRNA 表达式盒子,它能在一个轮由金门结扎或吉布森汇编克隆被装配进二进制 CRISPR/Cas9 向量。与这个系统,我们与 85.4% 变化评估的一般水准在米饭编辑了 46 个目标地点,主要在 biallelic 和同型结合的地位。我们推想在米饭的大约 16% 同型结合的变化通过相应基于再结合的修理跟随的非相应的加入结束的机制被产生。我们也获得了一致 biallelic,在 Arabidopsis T 1 植物的异质接合、同型结合、妄想的变化。在米饭和 Arabidopsis 的指向的变化是可继承的。我们在 T 0 米饭和 T 1 由同时的指向的 Arabidopsis 植物多重(多达八) 一个基因家庭,在一条 biosynthetic 小径的多重基因,或在单个基因的多重地点的成员。这个系统为为基本研究和基因改进在植物学习多重基因和基因家庭的功能提供了一个万用的工具箱。 | Xingliang Ma Qunyu Zhang Qinlong Zhu Wei Liu Yan Chen Rong Qiu Bin Wang Zhongfang Yang Heying Li Yuru Lin Yongyao Xie Rongxin Shen Shuifu Chen Zhi Wang Yuanling Chen Jingxin Guo Letian Chen Xiucai Zhao Zhicheng Dong Yao-Guang Liu | 2015 | Molecular Plant2015，8，8： | 272 |
| 2 | DSDecode: A Web-Based Tool for Decoding of Sequencing Chromatograms for Genotyping of Targeted Mutations显示文摘Dear Editor, The transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 genome editing systems have greatly improved the efficiency for generating targeted mutations in various organisms including plants (Li et al.,2012;Cong et al.,2013;Li et al.,2013;Feng et al.,2014;Ma et al.,2015b;Zhang et al.,2014,2015). | Weizhi Liu Xianrong Xie Xingliang Ma Jun Li Jiehu Chen Yao-Guang Liu | 2015 | Molecular Plant2015，8，9： | 62 |
| 3 | Structural insights into a novel histone demethylase PHF8显示文摘histone methylation/demethylation 的动态规定在开发期间起一个重要作用。在人的植物 homeodomain (哲学博士) 的变化和截断摸蛋白质 8 (PHF8 ) 与 X 连接智力迟钝和面部异例被联系,例如一张长脸,宽广鼻音尖端,劈开 lip/cleft 腭和大手,然而,它的分子的功能和结构的基础仍然保持不清楚。这里,我们在高分辨率报导 PHF8 的催化核心的水晶结构与或没有伪 - ketoglutarate (伪 - KG ) 。生物化学、结构的研究表明 PHF8 是为甲醇化物 di 、单音甲醇化物的 histone H3 离氨酸 9,然而并非为 H3K9me3 特定的新奇 histone demethylase (H3K9me2/1 ) 。我们的分析也揭示人的 PHF8 怎么在 methylation 状态之间区别并且为 methylated H3K9 完成顺序特性。在里面 vitro demethylation 试金也证明在临床的病人观察的 F279S 异种不拥有 demethylation 活动,建议酶的活动的那损失为 PHF8 病人的致病是关键的。一起拿,这些结果将使位于联系 PHF8 的发展、神经病学的疾病下面的分子的机制清楚些。 | Lin Yu Yang wang Shuo Huang Jianjun Wang Zengqin Deng Qi Zhang Wei Wu Xingliang Zhang Zhao Liu Weimin Gong Zhongzhou Chen | 2010 | Cell Research2010，20，2： | 11 |
| 4 | Improvement of the Semi-Lagrangian Advection Scheme in the GRAPES Model:Theoretical Analysis and Idealized Tests显示文摘The Global/Regional Assimilation and PrEdiction System(GRAPES) is the new-generation numerical weather prediction(NWP) system developed by the China Meteorological Administration. It is a fully compressible non-hydrostatical global/regional unified model that uses a traditional semi-Lagrangian advection scheme with cubic Lagrangian interpolation(referred to as the SL CL scheme). The SL CL scheme has been used in many operational NWP models, but there are still some deficiencies, such as the damping effects due to the interpolation and the relatively low accuracy. Based on Reich's semi-Lagrangian advection scheme(referred to as the R2007 scheme), the Re R2007 scheme that uses the low- and high-order B-spline function for interpolation at the departure point, is developed in this paper. One- and two-dimensional idealized tests in the rectangular coordinate system with uniform grid cells were conducted to compare the Re R2007 scheme and the SL CL scheme. The numerical results showed that:(1) the damping effects were remarkably reduced with the Re R2007 scheme; and(2) the normalized errors of the Re R2007 scheme were about 7.5 and 3 times smaller than those of the SL CL scheme in one- and two-dimensional tests, respectively, indicating the higher accuracy of the Re R2007 scheme.Furthermore, two solid-body rotation tests were conducted in the latitude–longitude spherical coordinate system with nonuniform grid cells, which also verified the Re R2007 scheme's advantages. Finally, in comparison with other global advection schemes, the Re R2007 scheme was competitive in terms of accuracy and flow independence. An encouraging possibility for the application of the Re R2007 scheme to the GRAPES model is provided. | HUANG Bo CHEN Dehui LI Xingliang LI Chao | 2014 | Advances in Atmospheric Sciences2014，31，3： | 8 |
| 5 | Carbon isotopes, sulfur isotopes, and trace elements of the dolomites from the Dengying Formation in Zhenba area, southern Shaanxi: Implications for shallow water redox conditions during the terminal Ediacaran显示文摘Carbon isotope, sulfur isotope, and trace element(including Rare Earth Elements, REE) analyses were conducted on the carbonates of the Dengying Formation at Lianghekou section in southern Shaanxi to reconstruct the terminal Ediacaran shallow-water environment on the northwestern margin of the Yangtze Platform. At Lianghekou section, samples in the middle 50-m of the Beiwan Member show characteristics of low ΣREE concentrations, no MREE-enriched REE distribution patterns, high Ce/Ce* values close to 1, and enriched redox-sensitive elements, whereas samples in the lower 30-m and upper 10-m show opposite characteristics of high ∑REE concentrations, MREE-enriched REE distribution patterns, low Ce/Ce* values around 0.6, and no redox-sensitive elements enriched, indicating that oxygenation did occur in the shallow water on the northwestern margin of the Yangtze Platform and redox conditions of the shallow water fluctuated from relatively oxygenated to anoxic and then back to oxygenated again. We propose that the anoxia appeared in middle of the Beiwan time may associate with the anoxic upwelled water. On one hand, abundant nutrients were brought in by this upwelling event, which stimulated the photosynthetic carbon fixation and increased the organic carbon burial under this anoxic condition, causing a peak of 3.6‰ in δ 13 C. On the other hand, because the anoxic upwelled water replaced the oxic shallow water, together with the increasing organic matter in the water column, bacterial sulfate reduction was enhanced and therefore quickly reduced the sulfate concentration, which eventually caused δ 34 S increasing to 50‰. However, as the upwelling gradually disappeared, δ 13 C and δ 34 SCAS values decreased as well in the late Beiwan time, indicating the shallow water went back to suboxic or oxic again. | CHEN YaLi CHU XueLei ZHANG XingLiang ZHAI MingGuo | 2015 | Science China Earth Sciences2015，58，7： | 6 |
| 6 | FT5a interferes with the Dt1-AP1 feedback loop to control flowering time and shoot determinacy in soybean显示文摘Flowering time and stem growth habit determine inflorescence architecture in soybean, which in turn influences seed yield. Dt1, a homolog of Arabidopsis TERMINAL FLOWER 1(TFL1), is a major controller of stem growth habit, but its underlying molecular mechanisms remain unclear.Here, we demonstrate that Dt1 affects node number and plant height, as well as flowering time,in soybean under long-day conditions. The b ZIP transcription factor FDc1 physically interacts with Dt1, and the FDc1-Dt1 complex directly represses the expression of APETALA1(AP1). We propose that FT5 a inhibits Dt1 activity via a competitive interaction with FDc1 and directly upregulates AP1. Moreover, AP1 represses Dt1 expression by directly binding to the Dt1 promoter, suggesting that AP1 and Dt1 form a suppressive regulatory feedback loop to determine the fate of the shoot apical meristem. These findings provide novel insights into the roles of Dt1 and FT5 a in controlling the stem growth habit and flowering time in soybean, which determine the adaptability and grain yield of this important crop. | Lin Yue Xiaoming Li Chao Fang Liyu Chen Hui Yang Jie Yang Zhonghui Chen Haiyang Nan Linnan Chen Yuhang Zhang Haiyang Li Xingliang Hou Zhicheng Dong James LWeller Jun Abe Baohui Liu Fanjiang Kong | 2021 | Journal of Integrative Plant Biology2021，63，6： | 3 |
| 7 | Black carbon decomposition and incorporation into soil microbial biomass estimated by 14 C labeling显示文摘 | Yakov Kuzyakov Irina Subbotina Haiqing Chen Irina Bogomolova Xingliang Xu | 2008 | Soil Biology and Biochemistry2008，，2： | 2 |
| 8 | Efficacy,safety and immunogenicity of hexavalent rotavirus vaccine in Chinese infants显示文摘A randomized,double-blind,placebo-controlled multicenter trial was conducted in healthy Chinese infants to assess the efficacy and safety of a hexavalent live human-bovine reassortant rotavirus vaccine(HRV)against rotavirus gastroenteritis(RVGE).A total of 6400 participants aged 6-12 weeks were enrolled and randomly assigned to either HRV(n?3200)or placebo(n?3200)group.All the subjects received three oral doses of vaccine four weeks apart.The vaccine efficacy(VE)against RVGE caused by rotavirus serotypes contained in HRV was evaluated from 14 days after three doses of administration up until the end of the second rotavirus season.VE against severe RVGE,VE against RVGE hospitalization caused by serotypes contained in HRV,and VE against RVGE,severe RVGE,and RVGE hospitalization caused by natural infection of any serotype of rotavirus were also investigated.All adverse events(AEs)were collected for 30 days after each dose.Serious AEs(SAEs)and intussusception cases were collected during the entire study.Our data showed that VE against RVGE caused by serotypes contained in HRV was 69.21%(95%CI:53.31-79.69).VE against severe RVGE and RVGE hospitalization caused by serotypes contained in HRV were 91.36%(95%CI:78.45-96.53)and 89.21%(95%CI:64.51-96.72)respectively.VE against RVGE,severe RVGE,and RVGE hospitalization caused by natural infection of any serotype of rotavirus were 62.88%(95%CI:49.11-72.92),85.51%(95%CI:72.74-92.30)and 83.68%(95%CI:61.34-93.11).Incidences of AEs from the first dose to one month post the third dose in HRV and placebo groups were comparable.There was no significant difference in incidences of SAEs in HRV and placebo groups.This study shows that this hexavalent reassortant rotavirus vaccine is an effective,well-tolerated,and safe vaccine for Chinese infants. | Zhiwei Wu Qingliang Li Yan Liu Huakun Lv Zhaojun Mo Fangjun Li Qingchuan Yu Fei Jin Wei Chen Yong Zhang Teng Huang Xiaosong Hu Wei Xia Jiamei Gao Haisong Zhou Xuan Bai Yueyue Liu Zhenzhen Liang Zhijun Jiang Yingping Chen Jiuwei Zhang Jialiang Du Biao Yang Bo Xing Yantao Xing Ben Dong Qinghai Yang Chen Shi Tingdong Yan Bo Ruan Haiyun Shi Xingliang Fan Dongyang Feng Weigang Lv Dong Zhang Xiangchu Kong Liuyifan Zhou Dinghong Que Hong Chen Zhongbing Chen Xiang Guo Weiwei Zhou Cong Wu Qingrong Zhou Yuqing Liu Jian Qiao Ying Wang Xinguo Li Kai Duan Yuliang Zhao Gelin Xu Xiaoming Yang | 2022 | Virologica Sinica2022，37，5： | 2 |
| 9 | Effect Evaluation of Collective Forestry Tenure Reform and Forest Farmers' Willingness to Participate in the Reform: A Case Study of Shaowu, Fujian Province显示文摘Since 2003, China has carried out the pilot of collective forest tenure reform (CFTR). Inrecent years, there have been lots of researches about evaluation of the CFTR, which are, however,mostly qualitative research with little focus on the quantitative research. This paper used the AnalyticHierarchy Process (AHP) and expert assignment method to define the comprehensive evaluation indexes and monitoring indexes of the CFTR. In this study, the authors did a quantitative evaluation of the CFTR in Shaowu, Fujian Province, which was scored as 0.844, indicating more significant effect. The authors investigated 100 farmer households there, and the data showed that the average household income has grown by 49.2% in the 6 years after the CFTR implementation, while the forestry incomehas grown by 108.3%, indicating that farmers’ income increased significantly after the CFTR. Factor analysis shows that CFTR has been the key factor to increase farmers’ income. | CHEN Xingliang WU Hailong YUAN Songsong | 2011 | Chinese Forestry Science and Technology2011，10，Z1： | 1 |
| 10 | Recent advances in molecularly imprinted polymers in food analysis显示文摘 | Xingliang Song Shoufang Xu Lingxin Chen Yingqin Wei Hua Xiong | 2014 | J. Appl. Polym. Sci2014，，16： | 1 |
| 11 | Y_(2)O_(3) nanosheets as slurry abrasives for chemical-mechanical planarization of copper显示文摘Continued reduction in feature dimension in integrated circuits demands high degree of flatness after chemical mechanical polishing.Here we report using new yttrium oxide(Y_(2)O_(3))nanosheets as slurry abrasives for chemical-mechanical planarization(CMP)of copper.Results showed that the global planarization was improved by 30%using a slurry containing Y_(2)O_(3) nanosheets in comparison with a standard industrial slurry.During CMP,the two-dimensional square shaped Y_(2)O_(3) nanosheet is believed to induce the low friction,the better rheological performance,and the laminar flow leading to the decrease in the within-wafer-non-uniformity,surface roughness,as well as dishing.The application of the two-dimensional nanosheets as abrasive in CMP would increase the manufacturing yield of integrated circuits. | Xingliang HE Yunyun CHEN Huijia ZHAO Haoming SUN Xinchun LU Hong LIANG | 2013 | Friction2013，1，4： | 1 |
| 12 | The Variation of Microbial(Methanotroph)Communities in Marine Sediments Due to Aerobic Oxidation of Hydrocarbons显示文摘Methanotrophs in marine sediments and overlying water attenuate the emissions of methane into the atmosphere and thus play an important role for the global cycle of this greenhouse gas.However,gas released from natural hydrocarbon seeps are not pure methane but commonly mixed hydrocarbons.Currently,how methanotrophic bacteria behave in the co-presence of methane and heavier hydrocarbons remains unknown.In this paper,the bacteria were cultured aerobically in fresh sediment samples(collected from Bohai Bay in eastern China)at 28℃under the atmospheres of pure methane and methane+ethane+propane mixed gas,respec-tively.The prevailing terrigenous n-alkanes and fatty acids in the original sediment samples varied consistently after incubations,confirming the proceeding of aerobic bacterial activities.The real-time quantitative PCR assay and sequencing of the 16S rRNA and particulate methane monooxygenase(pmoA)genes revealed the changes of microbe communities to a methanotroph-dominating structure after incubations.Particularly,after incubations the family Methylococcaceae(typeⅠmethanotrophs)became dominant with proportions higher than 40%,whereas Methylocystaceae(typeⅡmethanotrophs)nearly disappeared in all incubated samples.More-over,the species of methanotrophs from the samples treated with pure methane were dominated by Methylobacter luteus,whereas Methylobacter whittenburyi took the predominant proportion in the samples treated with mixed gas.The phenomenon suggests that some methanotrophs may also utilize ethane and propane.Collectively,this study may help to gain a better understanding of the ef-fects and contributions of microbial activities in marine hydrocarbon seep ecosystems. | LI Jing LIU Changling WU Nengyou HE Xingliang HAO Xiluo LI Feng CHEN Ye MENG Qingguo | 2021 | Journal of Ocean University of China2021，20，3： | 1 |
| 13 | Equivalence of influence of pollution simulating methods on DC flash-over stress of ice-covered insulators显示文摘 | JIANG Xingliang CHEN ling ZHANG Zhijin | 2010 | IEEE Transactions on Power Delivery2010，25，4： | 1 |
| 14 | A high-resolution cell atlas of the domestic pig lung and an online platform for exploring lung single-cell data显示文摘The genetically engineered pig is regarded as an optimal source of organ transplantation for humans and an excellent model for human disease research,given its comparable physiology to human beings.A myriad of single-cell RNA sequencing(sc RNA-seq)data on humans has been reported,but such data on pigs are scarce.Here,we apply sc RNA-seq technology to study the cellular heterogeneity of 3-month-old pig lungs,generating the single-cell atlas of 13,580 cells covering 16 major cell types.Based on these data,we systematically characterize the similarities and differences in the cellular cross-talk and expression patterns of respiratory virus receptors in each cell type of pig lungs compared with human lungs.Furthermore,we analyze pig lung xenotransplantation barriers and reported the cell-type expression patterns of 10 genes associated with pig-to-human immunobiological incompatibility and coagulation dysregulation.We also investigate the conserved transcription factors(TFs)and their candidate target genes and constructed five conserved TF regulatory networks in the main cell types shared by pig and human lungs.Finally,we present a comprehensive and openly accessible online platform,Scdb Lung.Our sc RNA-seq atlas of the domestic pig lung and Scdb Lung database can guide pig lung research and clinical applicability. | Lijing Zhang Jiacheng Zhu Haoyu Wang Jun Xia Ping Liu Fang Chen Hui Jiang Qiuling Miao Weiying Wu Lingli Zhang Lihua Luo Xiaosen Jiang Yong Bai Chengcheng Sun Dongsheng Chen Xingliang Zhang | 2021 | Journal of Genetics and Genomics2021，48，5： | 1 |
| 15 | Equivalence of influence of pollution simulating methods on DC flashover stress of ice-covered insulators 显示文摘 | Xingliang Jiang Ling Chen Zhijin Zhang | 2010 | IEEE Transactions on Power Delivery2010，25，4： | 1 |
| 16 | Secondary Phosphatization of the Earliest Cambrian Small Shelly Fossil Anabarites from Southern Shaanxi显示文摘Biomineralization may have an extremely long evolutionary history since the Paleoarchean, while the widespread biomineralization among metazoan lineages started at the earliest Cambrian. However, the primary mineralogy of Anabarites shell remains controversial. Optical microscopic observations combined with the Back-Scattered Electron(BSE) and Energy-Dispersive X-ray Spectroscopy(EDS) analyses are used to study the shell of the fossil Anabarites from the Kuanchuanpu fauna in southern Shaanxi Province in China, which is correlated to the Cambrian Fortunian Stage. The EDS analysis shows that the phosphorus-rich layer closely adjacent to the calcified layer exhibits a Ca: P: C ratio compositionally similar to the mineral fluorapatite(Ca_5(PO_4,CO_3)_3(F,CO_3). The result that the calcified layer and the phosphorus-rich layer have different chemical compositions is consistent with the optical observation that there is an obvious gap between these two layers and the phosphorus-rich layer can extend to the phosphatic material inside of the tube, suggesting the phosphorus-rich layer doesn't belong to the original shell. We suggest that the phosphorous-rich layer is diagenetic in origin, precipitated as a result of phosphorus release during the decay of organic matter by microbes. Considering the outermost shell layer(OMS, biologically controlled carbonate shell layer) should display different isotopic information from the carbonate matrix(i.e., OMS is ^(12)C concentrated due to the biogenic organic matter template is readily rich in ^(12)C), Nano SIMS was used to map ion distributions of C and N in the shell of Anabarites and matrix. However, ion images show that the concentration differences of ^(12)C, ^(13)C and ^(26)CN among the OMS and the matrix are unclear, while ^(12)C and ^(26)CN are supposed to be enriched in the OMS. Therefore, the minor isotopic differences between the shell and the matrix is hard to be detected by Nano SIMS, at least in our sample, probably due to alteration of the ^(12)C-rich characteristic of the Anabarites OMS during the late diagenesis. | Yali Chen Xuelei Chu Xingliang Zhang Mingguo Zhai | 2016 | Journal of Earth Science2016，27，2： | 1 |
| 17 | Combinatorial synthesis of redox-responsive cationic polypeptoids for intracellular protein delivery application显示文摘Biologics play an essential role in treating various indications from cancers to the metabolic diseases,while the current development of new classes of intracellular-acting protein drugs is still hindered because of high molecular mass and overall hydrophilicity of proteins creating extremely poor permeability across cell membrane.Hence,there remains an unmet need to develop safe,potent approaches to augment intracellular protein delivery efficiency.Here,we described a facile multicomponent reaction system for generating a small library of redox-responsive cationic polypeptoids with high biocompatibility.The co-assembly of optimized polymer with protein leads to the formation of compacted nanocomplexes with smaller size and high encapsulation efficiency,thus improving cellular internalization via the macropinocytosis and/or caveolae-mediated endocytosis mainly.After endo-lysosomal escape,the nanocomplexes can be disassociated to efficiently release cargo proteins into the cytosol,owing to the intracellular glutathione(GSH)-triggered rapid cleavage of disulfide bonds in polymers backbone.As a result,we screened a promising platform reagent for efficient cytosolic protein delivery application. | Zhicheng Le Tong Xiao Zhijia Liu Xingliang Liu Hong Liu Lixin Liu Yongming Chen | 2020 | Science China Chemistry2020，63，11： | 1 |
| 18 | Determination of polychlorinated biphenyls in seawater using headspacesolid-phase microextraction coupled with gas chromatography-mass spectrometry with the aid of experimental design显示文摘 | XINGLIANG SONG JINHUA LI LINGXIN CHEN | 2012 | J Braz Chem Soc2012，，： | 1 |
| 19 | Reinterpretation of Yunnanozoon as the earliest known hemichordate显示文摘 | SHU Degan ZHANG Xingliang CHEN Ling | 1996 | Nature1996，380，： | 1 |
| 20 | Determination of mannitoland threesugars in Ligustrum lucidum Ait by capillary electropho-resis with electrochemical detection显示文摘 | CHEN Gang ZHANG Luyan WU Xingliang | 2005 | Analytica Chimica Acta2005，530，1： | 1 |
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