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| 1 | Effectiveness of Jinying capsule on pelvic inflammatory disease in patients with symptom pattern of damp and heat accumulation: a double-blinded, multicenter, randomized, placebo-controlled clinical trial显示文摘OBJECTIVE: To evaluate the therapeutic effectiveness and safety of Jinying capsule on pelvic inflammatory disease(PID) in patients with symptoms identified as the pattern of damp and heat accumulation in terms of Traditional Chinese Medicine(TCM).METHODS: We conducted a double-blinded, multicenter, randomized, placebo-controlled clinical trial which included 155 patients diagnosed with PID and identified as symptom pattern of damp and heat accumulation. They were randomly divided into experimental group(n = 78) and control group(n = 77) according to a random number table. The treatment lasted for a period of 28 d. The experimental group was given Jinying capsules and oral levofloxacin plus oral metronidazole for first 7 d.They continued with Jinying capsules and levofloxacin placebo and metronidazole placebo for another 7 d. For the remaining 14 d, they continued with Jinying capsules only. Whereas, the control group was treated with oral levofloxacin and metronidazole and Jinying capsule placebo for the first 14 d in the same way as the experimental group and then continued with Jinying capsule placebo only for the remaining 14 d. The clinical efficacy was assessed using McCormack scale, TCM symptom pattern scores, physicochemical indexes including white blood cell and erythrocyte sedimentation rate, C-reaction protein, smear of vaginal discharge,and pelvic ultrasound.RESULTS: Comparing McCormack scale between both groups after treatment, the difference in curative effect between both groups was significant (P = 0.0269). The cure rate of the experimental group and control group is 76.32% and 59.46% respectively at week 4. Comparing TCM symptom pattern scores between both groups before and after treatment, the differences in total effective rate were both significant(P < 0.05). The curative effect rate of experimental group is 2.63% and 13.70% of the control group at week 1(P = 0.0131), and73.33% of the experimental group and 56.94% of the control group at week 4(P = 0.0369). No significant differences were found between the two groups on the Physicochemical indexes(all P >0.05). No adverse events or reactions occurred in the experimental groups.CONCLUSION: Jinying capsule can reduce the dosage of antibiotics needed for PID treatment, and improve the symptoms in PID patients. | Yang Yan Lu Yao Zhou Deping Pei Fenglan Li Qin Chen Shuqiong Wang Qiufeng Wang Yanting Jin Zhe Liu Yanxia | 2020 | Journal of Traditional Chinese Medicine2020，40，3： | 10 |
| 2 | Comparative Cyto-molecular Analysis of Repetitive DNA Provides Insights into the Differential Genome Structure and Evolution of Five Cucumis Species显示文摘The genus Cucumis, includes the cucumber(2n = 14), melon(2n = 24), and other wild species, which is a good model for studying genome organization and evolution due to their variation in genome size and basic chromosome number. In this study, five Cucumis species with different geographical origins and basic chromosome numbers(i.e., C. sativus, C. hystrix, C. melo, C. anguria, and C. metuliferus) were used to identify and characterize the repetitive DNA in detail using a phylogenetic method. Comparative cyto-molecular genetic analysis of repetitive DNA was carried out using a graph-based clustering method, construction of Neighbor-Joining tree and fluorescence in situ hybridization(FISH).The results revealed that the five Cucumis species had differences in the repeat content of their genome, as well as in the composition of repetitive DNA and their genomic proportions. Three species from subgenus Melo showed a decreased tendency in both repeat types and genomic proportions, while two species from subgenus Cucumis also showed a decreased tendency in repeat types, but an increased tendency in genomic proportions. Phylogenic analysis of Cucumis Ty1/Copia, Ty3/Gypsy, and 45 S rDNA ITS regions revealed that C. sativus, C. hystrix, and C. melo were closely related species, which C. sativus and C. hystrix were closer, while C. anguria and C. metuliferus were closer to each other and further from the other three species. Differential accumulation and elimination of different repeat types divergently shaped the genomic architecture of these five Cucumis species, contributing to the genome’s evolution and diversification. Overall, these results enhance our understanding of the genomes of these five Cucumis species, and contribute to a more holistic view of genome evolution and phylogenetics of this genus. | Shuqiong Yang Chunyan Cheng Xiaodong Qin Xiaqing Yu Qunfeng Lou Ji Li Chuntao Qian Jinfeng Chen | 2019 | Horticultural Plant Journal2019，5，5： | 2 |
| 3 | 显示文摘 | Chen Zhen Ma Lijian Li Shuqiong | 2011 | Applied Surface Science2011，257，86： | 1 |
| 4 | Expression of β-1,4-Galactosyltransferase-I in Rat during Inflammation显示文摘 | Ji Qian Chun Cheng Haiou Liu Jianping Chen Meijuan Yan Shuqiong Niu Jing Qin Linlin Sun Lei Liu Jianxin Gu Aiguo Shen | 2007 | Inflammation (-)2007，，1： | 1 |
| 5 | Magnetite-containing spherical silica nanoparticles for biocatalysis and bioseparations显示文摘 | Yang Huanghao Zhang Shuqiong Chen Xiaolan | 2004 | Analytical Chemistry2004，76，5： | 1 |
| 6 | Magnetite-containing spherical silica nanopartieles for bioeatalysis and bioseparations显示文摘 | YANG Huanghao ZHANG Shuqiong CHEN Xiaolan | 2004 | Anal Chem2004，76，： | 1 |
| 7 | Simple approach to carboxyl-rich materials through low-temperature heat treatment of hydrothermal carbon in air 显示文摘 | Zhen Chen Lijian Ma Shuqiong Li | 2011 | Applied Surface Science2011，257，20： | 1 |
| 8 | A comprehensive profile of TCF1^(+)progenitor and TCF1−terminally exhausted PD-1^(+)CD8^(+)T cells in head and neck squamous cell carcinoma:implications for prognosis and immunotherapy显示文摘The heterogeneity of exhausted T cells(Tex)is a critical determinant of immune checkpoint blockade therapy efficacy.However,few studies have explored exhausted T cell subpopulations in human cancers.In the present study,we examined samples from two cohorts of 175 patients with head and neck squamous cell cancer(HNSCC)by multiplex immunohistochemistry(mIHC)to investigate two subsets of Tex,CD8+PD1+TCF1+progenitor exhausted T cells(TCF1+Texprog)and CD8+PD1+TCF1−terminally exhausted T cells(TCF1−Texterm).Moreover,fresh tumor samples from 34 patients with HNSCC were examined by flow cytometry and immunohistochemistry to further investigate their properties and cytotoxic capabilities and their correlation with regulatory T cells(Tregs)in the tumor immune microenvironment(TIME).mIHC and flow cytometry analysis showed that TCF1−Texterm represented a greater proportion of CD8+PD1+Tex than TCF1+Texprog in most patients.TCF1+Texprog produced abundant TNFα,while TCF1−Texterm expressed higher levels of CD103,TIM-3,CTLA-4,and TIGIT.TCF1−Texterm exhibited a polyfunctional TNFα+GZMB+IFNγ+phenotype;and were associated with better overall survival and recurrence-free survival.The results also indicated that larger proportions of TCF1−Texterm were accompanied by an increase in the proportion of Tregs.Therefore,it was concluded that TCF1−Texterm was the major CD8+PD1+Tex subset in the HNSCC TIME and that these cells favor patient survival.A high proportion of TCF1−Texterm was associated with greater Treg abundance. | Dikan Wang Juan Fang Shuqiong Wen Qunxing Li Jinming Wang Lisa Yang Wenxiao Dai Huanzi Lu Junyi Guo Zhongyan Shan Wenqiang Xie Xiangqi Liu Liling Wen Jie Shen Anxun Wang Qianming Chen Zhi Wang | 2022 | International Journal of Oral Science2022，14，1： | 0 |
| 9 | Developmental expression of CAPON and Dexras1 in spinal cord of rats显示文摘To study the expression of the carboxy-ter-minal PSD-95/DLG/ZO-1 ligand of nNOS(CAPON)and Dexras1 mRNA during development in the spinal cord of rats,real-time polymerase chain reaction(Real-time PCR),as a quantitative method,was used to study the developmental expression of CAPON and Dexras1 mRNA level in the spinal cord.The spatial expression of CAPON and Dexras1 mRNA was examined by a com-bination of in situ hybridization(ISH)and immunofluor-escence.During the development of the spinal cord,CAPON mRNA was expressed in low levels from embryo day 14 to day 18.At postnatal day 1,it reached the peak and was expressed in the part which will become the dor-sal horn when mature.It then decreased gradually until postnatal week 12,when it presented in the ventral horn.At embryo day 14,Dexras1 mRNA was expressed at low levels,increased during embryo day 16 to day 18 and peaked at postnatal day 1.Spatiotemporal expression of Dexras1 mRNA was similar to CAPON as confirmed by correlation analysis and colocalization.CAPON and neuronal nitric oxide synthase(nNOS)was expressed within the same cells of the dorsal horn at postnatal day 1 but had different subcellular localizations.Co-express-ion of CAPON and Dexras1 mRNA in myeloid tissue during development process of rat indicates that the adaptor protein,CAPON may play a probable role in differentiation of neurons,synaptic plasticity and synap-togenesis by regulating nNOS to activate Dexras1. | Xin LI Chun CHENG Jian ZHAO Mengling CHEN Shuqiong NIU Shangfeng GAO Aiguo SHEN | 2008 | Frontiers of Medicine2008，2，1： | 0 |
| 10 | Chromosome-scale genome assembly of Cucumis hystrix-a wild species interspecifically cross-compatible with cultivated cucumber显示文摘Cucumis hystrix Chakr.(2n=2x=24)is a wild species that can hybridize with cultivated cucumber(C.sativus L.,2n=2x=14),a globally important vegetable crop.However,cucumber breeding is hindered by its narrow genetic base.Therefore,introgression from C.hystrix has been anticipated to bring a breakthrough in cucumber improvement.Here,we report the chromosome-scale assembly of C.hystrix genome(289 Mb).Scaffold N50 reached 14.1 Mb.Over 90%of the sequences were anchored onto 12 chromosomes.A total of 23,864 genes were annotated using a hybrid method.Further,we conducted a comprehensive comparative genomic analysis of cucumber,C.hystrix,and melon(C.melo L.,2n=2x=24).Whole-genome comparisons revealed that C.hystrix is phylogenetically closer to cucumber than to melon,providing a molecular basis for the success of its hybridization with cucumber.Moreover,expanded gene families of C.hystrix were significantly enriched in“defense response,”and C.hystrix harbored 104 nucleotide-binding site-encoding disease resistance gene analogs.Furthermore,121 genes were positively selected,and 12(9.9%)of these were involved in responses to biotic stimuli,which might explain the high disease resistance of C.hystrix.The alignment of whole C.hystrix genome with cucumber genome and self-alignment revealed 45,417 chromosome-specific sequences evenly distributed on C.hystrix chromosomes.Finally,we developed four cucumber-C.hystrix alien addition lines and identified the exact introgressed chromosome using molecular and cytological methods.The assembled C.hystrix genome can serve as a valuable resource for studies on Cucumis evolution and interspecific introgression breeding of cucumber. | Xiaodong Qin Zhonghua Zhang Qunfeng Lou Lei Xia Ji Li Mengxue Li Junguo Zhou Xiaokun Zhao Yuanchao Xu Qing Li Shuqiong Yang Xiaqing Yu Chunyan Cheng Sanwen Huang Jinfeng Chen | 2021 | Horticulture Research2021，8，1： | 0 |
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