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| 1 | Berberine mediates root remodeling in an immature tooth with apical periodontitis by regulating stem cells from apical papilla differentiation显示文摘Once pulp necrosis or apical periodontitis occurs on immature teeth,the weak root and open root apex are challenging to clinicians.Berberine(BBR)is a potential medicine for bone disorders,therefore,we proposed to apply BBR in root canals to enhance root repair in immature teeth.An in vivo model of immature teeth with apical periodontitis was established in rats,and root canals were filled with BBR,calcium hydroxide or sterilized saline for 3 weeks.The shape of the roots was analyzed by micro-computed tomography and histological staining.In vitro,BBR was introduced into stem cells from apical papilla(SCAPs).Osteogenic differentiation of stem cells from apical papilla was investigated by alkaline phosphatase activity,mineralization ability,and gene expression of osteogenic makers.The signaling pathway,which regulated the osteogenesis of SCAPs was evaluated by quantitative real time PCR,Western blot analysis,and immunofluorescence.In rats treated with BBR,more tissue was formed,with longer roots,thicker root walls,and smaller apex diameters.In addition,we found that BBR promoted SCAPs osteogenesis in a time-dependent and concentration-dependent manner.BBR induced the expression ofβ-catenin and enhancedβ-catenin entering into the nucleus,to up-regulate more runt-related nuclear factor 2 downstream.BBR enhanced root repair in immature teeth with apical periodontitis by activating the canonical Wnt/β-catenin pathway in SCAPs. | Yujia Cui Jing Xie Yujie Fu Chuwen Li Liwei Zheng Dingming Huang Changchun Zhou Jianxun Sun Xuedong Zhou | 2020 | International Journal of Oral Science2020，12，2： | 5 |
| 2 | NUPR1 promotes the proliferation and metastasis of oral squamous cell carcinoma cells by activating TFE3-dependent autophagy显示文摘Oral squamous cell carcinoma(OSCC)is the most common type of oral malignancy,and metastasis accounts for the poor prognosis of OSCC.Autophagy is considered to facilitate OSCC development by mitigating various cellular stresses;nevertheless,the mechanisms of autophagy in OSCC cell proliferation and metastasis remain unknown.In our study,high-sensitivity label-free quantitative proteomics analysis revealed nuclear protein 1(NUPR1)as the most significantly upregulated protein in formalin-fixed paraffin-embedded tumour samples derived from OSCC patients with or without lymphatic metastasis. | Tengfei Fan Xiaoning Wang Sheng Zhang Ping Deng Yi Jiang Yidan Liang Sheng Jie Qing Wang Chuwen Li Guocai Tian Zhen Zhang Zhenhu Ren Bo Li Yanrong Chen Zhijing He Yan Luo Mingliang Chen Hanjiang Wu Zhengping Yu Huifeng Pi Zhou Zhou Zhiyuan Zhang | 2022 | Signal Transduction and Targeted Therapy2022，7，5： | 2 |
| 3 | Isolation and Identification of Serotype O101 Bovine Pathogenic Escherichia coli with Multidrug Resistance显示文摘[Objective]The paper was to isolate and identify a multidrug-resistance bovine pathogenic Escherichia coli. [Method]The dead cases of calf diarrhea were collected from a large-scale beef cattle farm,and the isolated pathogen was conducted molecular identification,serological identification,drug sensitivity test,and mice pathogenicity test,respectively. Targeted therapy was undertaken thereafter to herds. [Result] One strain of bovine pathogenic E. coli,serotype O101 with strong multidrug resistance and high pathogenicity to mice,was successfully isolated. It was used to develop sensitive drug for timely treating follow-up diarrhea calves,and successfully controlled calf diarrhea in the farm. [Conclusion]The results provide a basis for effective prevention and control of bovine colibacillosis. | Li Shuguang Liu Xiaoyun Wang Yumao Li Feng Lin Chuwen Shen Zhiqiang Li Jinlin | 2017 | Animal Husbandry and Feed Science2017，9，5： | 1 |
| 4 | Spontaneous rupture of hepatocellular carcinoma trans-arterial embolization or hepatectomy?显示文摘Spontaneous rupture and hemorrhage of hepatocellular carcinoma(HCC)is one of the serious complications leading to death in patients with primary liver cancer.Approximately 9-10%of those patients with liver cancer die of from spontaneous rupture and hemorrhage,which is often accompanied by abdominal metastasis and seriously affects their prognosis(1). | Qingbo Feng Xiaoyin Liu Xuping Feng Chuwen Chen Jiaxin Li Yong Zeng | 2022 | Hepatobiliary Surgery and Nutrition2022，11，6： | 1 |
| 5 | Candidate therapeutic agents in a newly established triple wild-typemucosalmelanoma cell line显示文摘Background:Mucosalmelanoma has characteristically distinct genetic features and typically poor prognosis.The lack of representativemucosal melanoma models,especially cell lines,has hindered translational research on this melanoma subtype.In this study,we aimed to establish and provide the biological properties,genomic features and the pharmacological profiles of a mucosal melanoma cell line that would contribute to the understanding and treatment optimization of molecularly-defined mucosal melanoma subtype.Methods:The sample was collected from a 67-year-old mucosal melanoma patient and processed into pieces for the establishment of cell line and patientderived xenograft(PDX)model.The proliferation and tumorigenic property of cancer cells from different passageswere evaluated,andwhole-genome sequencing(WGS)was performed on the original tumor,PDX,established cell line,and the matched blood to confirm the establishment and define the genomic features of this cell line.AmpliconArchitect was conducted to depict the architecture of amplified regions detected by WGS.High-throughput drug screening(HTDS)assay including a total of 103 therapeutic agents was implemented on the established cell line,and selected candidate agents were validated in the corresponding PDX model.Results:A mucosal melanoma cell line,MM9H-1,was established which exhibited robust proliferation and tumorigenicity after more than 100 serial passages.Genomic analysis of MM9H-1,corresponding PDX,and the original tumor showed genetic fidelity across genomes,and MM9H-1 was defined as a triple wild-type(TWT)melanoma subtype lacking well-characterized“driver mutations”.Instead,the amplification of several oncogenes,telomerase reverse transcriptase(TERT),v-Rafmurine sarcoma viral oncogene homolog B1(BRAF),melanocyte Inducing transcription factor(MITF)and INO80 complex ATPase subunit(INO80),via large-scale genomic rearrangement potentially contributed to oncogenesis of MM9H-1.Moreover,HTDS identified proteasome inhibitors,especially bortezomib,as promising therapeutic candidates for MM9H-1,which was verified in the corresponding PDX model in vivo.Conclusions:We established and characterized a new mucosal melanoma cell line,MM9H-1,and defined this cell line as a TWT melanoma subtype lacking well-characterized“driver mutations”.The MM9H-1 cell line could be adopted as a unique model for the preclinical investigation of mucosal melanoma. | Chaoji Shi Ziyue Gu Shengming Xu Houyu Ju Yunteng Wu Yong Han Jiayi Li Chuwen Li Jing Wu Lizhen Wang Jiang Li Guoyu Zhou Weimin Ye Guoxin Ren Zhiyuan Zhang Rong Zhou | 2022 | Cancer Communications2022，42，7： | 1 |
| 6 | Integrated'omics analysis for the gut microbiota response to moxibustion in a rat model of chronic fatigue syndrome显示文摘OBJECTIVE:To observe the efficacy of moxibustion in the treatment of chronic fatigue syndrome(CFS)and explore the effects on gut microbiota and metabolic profiles.METHODS:Forty-eight male Sprague-Dawley rats were randomly assigned to control group(Con),CFS model group(Mod,established by multiple chronic stress for 35 d),MoxA group(CFS model with moxibustion Shenque(CV8)and Guanyuan(CV4),10 min/d,28 d)and MoxB group(CFS model with moxibustion Zusanli(ST36),10 min/d,28 d).Open-field test(OFT)and Morris-water-maze test(MWMT)were determined for assessment the CFS model and the therapeutic effects of moxibustion.16S rRNA gene sequencing analysis based gut microbiota integrated untargeted liquid chromatograph-mass spectrometer(LC-MS)based fecal metabolomics were executed,as well as Spearman correlation analysis,was utilized to uncover the functional relevance between the potential metabolites and gut microbiota.RESULTS:The results of our behavioral tests showed that moxibustion improved the performance of CFS rats in the OFT and the MWMT.Microbiome profiling analysis revealed that the gut microbiomes of CFS rats were less diverse with altered composition,including increases in pro-inflammatory species(such as Proteobacteria)and decreases in anti-inflammatory species(such as Bacteroides,Lactobacillus,Ruminococcus,and Prevotella).Moxibustion partially normalized these changes in the gut microbiota.Furthermore,CFS was associated with metabolic disorders,which were effectively ameliorated by moxibustion.This was demonstrated by the normalization of 33 microbiota-related metabolites,including mannose(P=0.001),aspartic acid(P=0.009),alanine(P=0.007),serine(P=0.000),threonine(P=0.027),methionine(P=0.023),5-hydroxytryptamine(P=0.008),alpha-linolenic acid(P=0.003),eicosapentaenoic acid(P=0.006),hypoxanthine(P=0.000),vitamin B6(P=0.000),cholic acid(P=0.013),and taurocholate(P=0.002).Correlation analysis showed a significant association between the perturbed fecal microbiota and metabolite levels,with a notable negative relationship between LCA and Bacteroides.CONCLUSIONS:In this study,we demonstrated that moxibustion has an antifatigue-like effect.The results from the 16S rRNA gene sequencing and metabolomics analysis suggest that the therapeutic effects of moxibustion on CFS are related to the regulation of gut microorganisms and their metabolites.The increase in Bacteroides and decrease in LCA may be key targets for the moxibustion treatment of CFS. | LI Chaoran YANG Yan FENG Chuwen LI Heng QU Yuanyuan WANG Yulin WANG Delong WANG Qingyong GUO Jing SHI Tianyu SUN Xiaowei WANG Xue HOU Yunlong SUN Zhongren YANG Tiansong | 2023 | Journal of Traditional Chinese Medicine2023，43，6： | 0 |
| 7 | Correction to:NUPR1 promotes the proliferation and metastasis of oral squamous cell carcinoma cells by activating TFE3-dependent autophagy显示文摘In the process of checking the raw data1,the authors noticed several inadvertent mistakes occurring in Fig.5a,b,c,d,f that need to be corrected after online publication of the article.During the preparation of Fig.5,the representative image showing TFE3 overexpression antagonized the NUPR1 KD-induced inhibition of OSCC cell proliferation and metastasis,were pasted and placed by mistake.The correct results should be as shown below.The authors apologize for these oversights and declare that these corrections do not affect the description,interpretation,or conclusions detailed in the original manuscript. | Tengfei Fan Xiaoning Wang Sheng Zhang Ping Deng Yi Jiang Yidan Liang Sheng Jie Qing Wang Chuwen Li Guocai Tian Zhen Zhang Zhenhu Ren Bo Li Yanrong Chen Zhijing He Yan Luo Mingliang Chen Hanjiang Wu Zhengping Yu Huifeng Pi Zhou Zhou Zhiyuan Zhang | 2022 | Signal Transduction and Targeted Therapy2022，7，10： | 0 |
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