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| 1 | Reactivation of γ-globin expression through Cas9 or base editor to treat β-hemoglobinopathies显示文摘Dear Editor,Mutations in the β-globin gene,the essential component of adult hemoglobin(HbA;a2p2),results in either a production of aberrant sickle hemoglobin(HbS)leading to sickle cell disease(SCD)or an insufficient β-globin synthesis leading to β-thalassemia.These two major forms of β-hemoglobinopathies cause impaired erythropoiesis and life-threatening anemia.Clinical evidence has suggested that reaaivation of fetal γ-globin(HBG)gene expression which is normally silenced after birth by certain genetic mutations can ameliorate the clinical course of β-hemoglobinopathies. | Liren Wang Linxi Li Yanlin Ma Handan Hu Qi Li Yang Yang Wenbang Liu Shuming Yin Wei Li Bin Fu Ryo Kurita Yukio Nakamura Mingyao Liu Yongrong Lai Dali Li | 2020 | Cell Research2020，30，3： | 14 |
| 2 | Genetic Analysis and Fine Mapping of Two Genes for Grain Shape and Weight in Rice显示文摘To identify genetic loci controlling grain weight,an elite indica rice variety,Baodali,with large grains was identified and used in this study.Its derived F2,F3 and BC2F2 with another japonica rice variety Zhonghua 11 were used as mapping populations.Linkage analyses demonstrated that two genes controlling grain weight,designated as GW3 and GW6,were mapped to chromosome 3 and chromosome 6,respectively.Fine mapping delimited GW3 to a 122 kb physical distance between two sequence tagged site markers(WGW16 and WGW19) containing 16 open reading frames annotated by The Institute for Genomic Research(http://www.tigr.org).GW6 was further mapped between two simple sequence repeat markers(RM7179 and RM3187).These results are useful for both marker assisted selection of grain weight,and for further cloning of GW genes,which will contribute to the dissection of the molecular mechanism underlying grain weight in rice. | Longbiao Guo Lilian Ma Hua Jiang Dali Zeng Jiang Hu Liwen Wu Zhenyu Gao Guangheng Zhang Qian Qian | 2009 | Journal of Integrative Plant Biology2009，51，1： | 13 |
| 3 | Characterization and cloning of a brittle culm mutant (bc88) in rice (Oryza sativa L.)显示文摘This study characterizes a brittle culm (bc88) mutant of rice (Oryza sativa L.) obtained by ethylene methylsulfonate (EMS)-induced mutagenesis of Wuyunjing 7. The bc88 mutant exhibits a diversity of pleiotropic phenotypes, including brittle culm at the whole-plant growth stages, withered leaf tips at the seedling stage, and 18-d delay in heading date at the mature stage. Genetic analysis indicates that the bc88 mutant is controlled by a single recessive nuclear gene. The mutated bc88 gene isolated by map-based cloning contains only one point mutation in the 5th exon relative to its wild-type BC88 (LOC_Os09g25490 and Os09g0422500), leading to an amino acid change from P to L in bc88 plants. Alignment of the putative protein sequence with its homologs indicates that the mutation is located in the conserved region of the sequence. Detection of the transcription level of BC88 in rice plants shows that the expression level of BC88 is higher in spikes and culms than in leaves, roots, and leaf sheaths. These contribute to understanding of the molecular mechanism of cellulose synthesis. The target gene BC88 can be a useful tool in molecular marker-assisted selection for rice culm trait breeding. | RAO YuChun YANG YaoLong XIN DeDong LI XiaoJing ZHAI KaiEn MA BoJun PAN JianWei QIAN Qian ZENG DaLi | 2013 | Chinese Science Bulletin2013，58，24： | 11 |
| 4 | Xa7, a new executor R gene that confers durable and broad-spectrum resistance to bacterial blight disease in rice显示文摘Bacterial blight(BB)is a globally devastating rice disease caused by Xanthomonas oryzae pv.oryzae(Xoo).The use of disease resistance(R)genes in rice breeding is an effective and economical strategy for the control of this disease.Nevertheless,a majority of R genes lack durable resistance for long-term use under global warming conditions.Here,we report the isolation of a novel executor R gene,Xa7,that confers extremely durable,broad-spectrum,and heat-tolerant resistance to Xoo.The expression of Xa7 was induced by incompatible Xoo strains that secreted the transcription activator-like effector(TALE)AvrXa7 or PthXo3,which recognized effector binding elements(EBEs)in the Xa7 promoter.Furthermore,Xa7 induction was faster and stronger under high temperatures.Overexpression of Xa7 or co-transformation of Xa7 with avrXa7 triggered a hypersensitive response in plants.Constitutive expression of Xa7 activated a defense response in the absence of Xoo but inhibited the growth of transgenic rice plants.In addition,analysis of over 3000 rice varieties showed that the Xa7 locuswas found primarily in the indica and aus subgroups.A variation consisting of an 11-bp insertion and a base substitution(G to T)was found in EBEAvrXa7 in the tested varieties,resulting in a loss of Xa7 BB resistance.Through a decade of effort,we have identified an important BB resistance gene and characterized its distinctive interaction with Xoo strains;these findings will greatly facilitate research on the molecular mechanism of Xa7-mediated resistance and promote the use of this valuable gene in breeding. | Xifeng Chen Pengcheng Liu Le Mei Xiaoling He Long Chen Hui Liu Shurong Shen Zhandong Ji Xixi Zheng Yuchen Zhang Zhenyu Gao Dali Zeng Qian Qian Bojun Ma | 2021 | Plant Communications2021，2，3： | 9 |
| 5 | Quantitative Trait Loci for Panicle Layer Uniformity Identified in Doubled Haploid Lines of Rice in Two Environments显示文摘Uniformity of stem height in rice directly affects crop yield potential and appearance,and has become a vital index for rice improvement.In the present study,a doubled haploid(DH) population,derived from a cross between japonica rice Chunjiang 06 and indica rice TN1 was used to analyze the quantitative trait locus(QTL) for three related traits of paniclelayer-uniformity;that is,the tallest panicle height,the lowest panicle height and panicle layer disuniformity in two locations:Hangzhou(HZ) and Hainan(HN).A total of 16 QTLs for three traits distributed on eight chromosomes were detected in two different environments.Two QTLs,qTPH-4 and qTPH-8 were co-located with the QTLs for qLPH-4 and qLPH-8,which were only significant in the HZ environment,whereas the qTPH-6 and qLPH-6 located at the same interval were only significant in the HN environment.Two QTLs,qPLD-10-1 and qPLD-10-2,were closely linked to qTPH-10,and they might have been at the same locus.One QTL,qPLD-3,was detected in both environments,explaining more than 23% of the phenotypic variations.The CJ06 allele of qPLD-3 could increase the panicle layer disuniformity by 9.23 and 4.74 cm in the HZ and HN environments.Except for qPLD-3,almost all other QTLs for the same trait were detected only in one environment,indicating that these three traits were dramatically affected by environmental factors.The results may be useful for elucidation of the molecular mechanism of panicle-layer-uniformity and marker assisted breeding for super-rice. | Liangyong Ma Jinsong Bao Longbiao Guo Dali Zeng Ximing Li Zhijuan Ji Yingwu Xia Changdeng Yang Qian Qian | 2009 | Journal of Integrative Plant Biology2009，51，9： | 7 |
| 6 | Methane dehydroaromatization with periodic CH_4-H_2 switch:A promising process for aromatics and hydrogen显示文摘Long-term stability test of Mo/HZSM-5-N catalysts(HZSM-5-N stands for nano-sized HZSM-5) in methane dehydroaromatization(MDA)reaction has been performed with periodic CH4-H2 switch at 1033-1073 K for more than 1000 h.During this test,methane conversion ranges from 13% to 16%,and mean yield to aromatics(i.e.benzene and naphthalene) exceeds 10%.N2-physisorption,XRD,NMR and TPO measurements were performed for the used Mo/HZSM-5 catalysts and coke deposition,and the results revealed that the periodic hydrogenation can effectively suppress coke deposition by removing the inert aromatic-type coke,thus ensuring Mo/HZSM-5 partly maintained its activity even in the presence of large amount of coke deposition.The effect of zeolite particle size on the catalytic activity was also explored,and the results showed that the nano-sized zeolite with low diffusion resistance performed better.It is recognized that the size effect was enhanced by reaction time,and it became more remarkable in a long-term MDA reaction even at a low space velocity. | Changyong Sun Guangzong Fang Xiaoguang Guo Yuanli Hu Shuqi Ma Tianhua Yang Jie Han Hao Ma Dali Tan Xinhe Bao | 2015 | Journal of Energy Chemistry2015，24，3： | 6 |
| 7 | Regulation of phenylpropanoid biosynthesis by MdMYB88 and MdMYB124 contributes to pathogen and drought resistance in apple显示文摘MdMYB88 and MdMYB124 have been demonstrated to be responsible for lignin accumulation in apple under drought stress.In this study,using a metabolomic approach,we identified differentially accumulated phenylpropanoid and flavonoid metabolites in MdMYB88/124 transgenic RNAi plants under control and long-term drought stress conditions in apple roots.We confirmed the regulation of phenylalanine by MdMYB88 and MdMYB124 via UPLC-MS in apple roots under both control and drought conditions.Using Electrophoretic Mobility Shift Assay(EMSA)and ChIPquantitative PCR(qPCR)analyses,we found that MdMYB88 positively regulates the MdCM2 gene,which is responsible for phenylalanine biosynthesis,through binding to its promoter region.Under long-term drought conditions,MdMYB88/124 RNAi plants consistently accumulated increased amounts of H2O2 and MDA,while MdMYB88 and MdMYB124 overexpression plants accumulated decreased amounts of H2O2 and MDA.We also examined the accumulation of metabolites in the phenylpropanoid biosynthesis pathway in the leaves of MdMYB88 and MdMYB124 transgenic apple plants after long-term drought stress.We found that metabolites responsible for plant defense,including phenylpropanoids and flavonoids,accumulated less in the RNAi plants but more in the overexpression plants under both control and drought conditions.We further demonstrated that MdMYB88/124 RNAi plants were more sensitive to Alternaria alternata f.sp.mali and Valsa mali,two pathogens that currently severely threaten apple production.In contrast,MdMYB88 and MdMYB124 overexpression plants were more tolerant to these pathogens.The cumulative results of this study provided evidence for secondary metabolite regulation by MdMYB88 and MdMYB124,further explained the molecular roles of MdMYB88 and MdMYB124 in drought resistance,and provided information concerning molecular aspects of their roles in disease resistance. | Dali Geng Xiaoxia Shen Yinpeng Xie Yusen Yang Ruiling Bian Yuqi Gao Pengmin Li Liying Sun Hao Feng Fengwang Ma Qingmei Guan | 2020 | Horticulture Research2020，7，1： | 5 |
| 8 | miR-155 aggravates impaired autophagy of pancreatic acinar cells through targeting Rictor显示文摘The aim of this study was to investigate the role and mechanism of miR-155 in regulating autophagy in a caerulein-induced acute pancreatitis(AP)cellular model.GFP-LC3 immunofluo-rescence assay was performed to detect autophagy vesicle formation in pancreatic acinar cell line AR42J.AR42J cells were transfected with miR-155 mimic,inhibitor,and corresponding controls to explore the effect of miR-155 on autophagy.The protein levels of LC3-I,LC3-11,Beclin-1,and p62 were analyzed by western blot analysis.Dual-luciferase reporter assay was performed to verify the interaction between miR-155 and Rictor(RPTOR independent companion of MTOR complex 2).The results showed that caerulein treatment induced impaired autophagy as evi-denced by an increase in the accumulation of p62 together with LC3-11 in AR42J cells,accompanied by miR-155 upregulation.Furthermore,miR-155 overexpression aggravated,whereas miR-155 silencing reduced the caerulein-induced impairment of autophagy.Mechanistically,Rictor was confirmed to be a direct target of miR-155,which could rescue the miR-155 overexpression-mediated aggravation of im paired autophagy.Collectively,these findings indicate that miR-155 aggravates impaired autophagy in caerulein-treated pancreatic acinar cells by targeting Rictor. | Xueming Zhang Jiangtao Chu Haijun Sun Dali Zhao Biao Ma Dongbo Xue Weihui Zhang Zhituo Li | 2020 | Acta Biochimica et Biophysica Sinica2020，52，2： | 4 |
| 9 | The Global Landscape of SARS-CoV-2 Genomes, Variants, and Haplotypes in 2019nCoVR显示文摘On January 22,2020,China National Center for Bioinformation(CNCB)released the 2019 Novel Coronavirus Resource(2019nCoVR),an open-access information resource for the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).2019nCoVR features a comprehensive integration of sequence and clinical information for all publicly available SARS-CoV-2 isolates,which are manually curated with value-added annotations and quality evaluated by an automated in-house pipeline.Of particular note,2019nCoVR offers systematic analyses to generate a dynamic landscape of SARS-CoV-2 genomic variations at a global scale.It provides all identified variants and their detailed statistics for each virus isolate,and congregates the quality score,functional annotation,and population frequency for each variant.Spatiotemporal change for each variant can be visualized and historical viral haplotype network maps for the course of the outbreak are also generated based on all complete and high-quality genomes available.Moreover,2019nCoVR provides a full collection of SARS-CoV-2 relevant literature on the coronavirus disease 2019(COVID-19),including published papers from PubMed as well as preprints from services such as bioRxiv and medRxiv through Europe PMC.Furthermore,by linking with relevant databases in CNCB,2019nCoVR offers data submission services for raw sequence reads and assembled genomes,and data sharing with NCBI.Collectively,SARS-CoV-2 is updated daily to collect the latest information on genome sequences,variants,haplotypes,and literature for a timely reflection,making 2019nCoVR a valuable resource for the global research community.2019nCoVR is accessible at https://bigd.big.ac.cn/ncov/. | Shuhui Song Lina Ma Dong Zou Dongmei Tian Cuiping Li Junwei Zhu Meili Chen Anke Wang Yingke Ma Mengwei Li Xufei Teng Ying Cui Guangya Duan Mochen Zhang Tong Jin Chengmin Shi Zhenglin Du Yadong Zhang Chuandong Liu Rujiao Li Jingyao Zeng Lili Hao Shuai Jiang Hua Chen Dali Han Jingfa Xiao Zhang Zhang Wenming Zhao Yongbiao Xue Yiming Bao | 2020 | Genomics, Proteomics & Bioinformatics2020，18，6： | 4 |
| 10 | Generation of obese rat model by transcription activator-like effector nucleases targeting the leptin receptor gene显示文摘The laboratory rat is a valuable mammalian model organism for basic research and drug discovery. Here we demonstrate an efficient methodology by applying transcription activator-like effector nucleases(TALENs) technology to generate Leptin receptor(Lepr) knockout rats on the Sprague Dawley(SD) genetic background. Through direct injection of in vitro transcribed m RNA of TALEN pairs into SD rat zygotes, somatic mutations were induced in two of three resulting pups. One of the founders carrying bi-allelic mutation exhibited early onset of obesity and infertility. The other founder carried a chimeric mutation which was efficiently transmitted to the progenies. Through phenotyping of the resulting three lines of rats bearing distinct mutations in the Lepr locus, we found that the strains with a frame-shifted or premature stop codon mutation led to obesity and metabolic disorders. However, no obvious defect was observed in a strain with an in-frame 57 bp deletion in the extracellular domain of Lepr. This suggests the deleted amino acids do not significantly affect Lepr structure and function. This is the first report of generating the Lepr mutant obese rat model in SD strain through a reverse genetic approach. This suggests that TALEN is an efficient and powerful gene editing technology for the generation of disease models. | Yuting Chen Wenqing Lu Na Gao Yi Long Yanjiao Shao Meizhen Liu Huaqing Chen Shixin Ye Xueyun Ma Mingyao Liu Dali Li | 2017 | Science China(Life Sciences)2017，60，2： | 4 |
| 11 | Tonoplast-associated calcium signaling regulates manganese homeostasis in Arabidopsis显示文摘Manganese(Mn)is an essential micronutrient in plants.However,excessive Mn absorption in acidic and waterlogged soils can lead to Mn toxicity.Despite their essential roles in Mn homeostasis,transcriptional and post-transcriptional modifications of Mn transporters remain poorly understood.Here,we demonstrated that high-Mn stress induces an obvious Ca^(2+) signature in Arabidopsis.We identified four calcium-dependent protein kinases,CPK4/5/6/11,that interact with the tonoplast-localized Mn and iron(Fe)transporter MTP8 in vitro and in vivo.The cpk4/5/6/11 quadruple mutant displayed a dramatic high-Mn-sensitive phenotype similar to that of the mtp8 mutant.CPKs phosphorylated the N-terminal domain of MTP8 primarily at the Ser31 and Ser32 residues.Transport assays combined with multiple physiological experiments on phospho-dead variant MTP8^(S31/32A)and phospho-mimetic variant MTP8^(S31/32D)plants under different Mn and Fe conditions suggested that Ser31 and Ser32 are crucial for MTP8 function.In addition,genetic analysis showed that CPKs functioned upstream of MTP8.In summary,we identified a tonoplast-associated calcium signaling cascade that orchestrates Mn homeostasis and links Mn toxicity,Ca^(2+) signaling,and Mn transporters.These findings provide new insight into Mn homeostasis mechanisms and Ca^(2+) signaling pathways in plants,providing potential targets for engineering heavy metal toxicity-tolerant plants. | Zhenqian Zhang Dali Fu Zhihui Sun Chuanfeng Ju Cuicui Miao Zhangqing Wang Dixiang Xie Liang Ma Zhizhong Gong Cun Wang | 2021 | Molecular Plant2021，14，5： | 2 |
| 12 | Mapping QTLs for heading synchrony in a doubled haploid population of rice in two environments显示文摘Simultaneous heading of plants within the same rice variety, also refer to heading synchrony, is an important factor that affects simultaneous ripening of the variety. Understanding of the genetic basis of heading synchrony may contribute to molecular breeding of rice with simultaneous heading and ripening. In the present study, a doubled haploid (DH) population, derived from a cross between Chunjiang 06 and TN1 was used to analyze quantitative trait locus (QTL) for heading synchrony related traits, i.e., early heading date (EHD), late heading date (LHD), heading asynchrony (HAS), and tiller number (PN). A total of 19 QTLs for four traits distributed on nine chromosomes were detected in two environments. One QTL, qHAS-8 for HAS, explained 27.7% of the phenotypic variation, co-located with the QTLs for EHD and LHD, but it was only significant under long-day conditions in Hangzhou, China. The other three QTLs, qHAS-6, qHAS-9, and qHAS-10, were identified under short-day conditions in Hainan, China, each of which explained about 11% of the phenotypic variation. Two of them, qHAS-6 and qHAS-9, were co-located with the QTLs for EHD and LHD. Two QTLs, qPN-4 and qPN-5 for PN, were detected in Hangzhou, and qPN-5 was also detected in Hainan. However, none of them was co-located with QTLs for EHD, LHD, and HAS, suggesting that PN and HAS were controlled by different genetic factors. The results of this study can be useful in marker assisted breeding for improvement of heading synchrony. | Liangyong Ma Changdeng Yang Dali Zeng Jing Cai Ximing Li Zhijuan Ji Yingwu Xia Qian Qian Jinsong Bao | 2009 | Journal of Genetics and Genomics2009，36，5： | 2 |
| 13 | Mn,N co-doped Co nanoparticles/porous carbon as air cathode for highly efficient rechargeable Zn-air batteries Hide Author's Information显示文摘Oxygen reduction reaction(ORR)and oxygen evolution reaction(OER)are generally catalyzed by precious metals(Pt)and metal oxides(IrO_(2))which still have many shortages including expensive price,poor selectivity and undesirable stability.In this work,we report a Mn0-doped CoN_(x) on N-doped porous carbon(Mn-CoN_(x)/N-PC)composite from carbonizing metal-organic framework(MOF)derivative as the dual-functional catalyst to boost both the ORR and OER performances.Owing to the strong coordination effect between nitrogen and metal elements,the introduction of N can obviously improve the content of Co-N-C active sites for ORR.Meanwhile,the Mn-doping significantly regulates the electronic structure of the Co element and increases the content of Co^(0) which provide efficient OER active sites.Mn-CoN_(x)/N-PC catalyst delivers super dual-functional activity with a half-wave potential of 0.85 V,better than the 20%Pt/C catalyst(0.82 V).When used in Zn-air batteries for testing,Mn-CoN_(x)/N-PC electrocatalyst shows a high power density(145 mW·cm^(−2))and good cycle performance. | Hanzhen Zheng Fei Ma Hongcen Yang Xiaogang Wu Rui Wang Dali Jia Zhixia Wang Niandi Lu Fen Ran Shanglong Peng | 2022 | Nano Research2022，15，3： | 2 |
| 14 | Genome-wide identification of droughtresponsive microRNAs in two sets of Malus from interspecific hybrid progenies显示文摘Drought stress can negatively impact apple fruit quality and yield.Apple microRNAs(miRNAs)participate in apple tree and fruit development,as well as in biotic stress tolerance;however,it is largely unknown whether these molecules are involved in the drought response.To identify drought-responsive miRNAs in Malus,we first examined the drought stress tolerance of ten F1 progenies of R3(M.×domestica)×M.sieversii.We performed Illumina sequencing on pooled total RNA from both drought-tolerant and drought-sensitive plants.The sequencing results identified a total of 206 known miRNAs and 253 candidate novel miRNAs from drought-tolerant plants and drought-sensitive plants under control or drought conditions.We identified 67 miRNAs that were differentially expressed in drought-tolerant plants compared with drought-sensitive plants under drought conditions.Under drought stress,61 and 35 miRNAs were differentially expressed in drought-tolerant and drought-sensitive plants,respectively.We determined the expression levels of seven out of eight miRNAs by stem-loop qPCR analysis.We also predicted the target genes of all differentially expressed miRNAs and identified the expression of some genes.Gene Ontology analyses indicated that the target genes were mainly involved in stimulus response and cellular and metabolic processes.Finally,we confirmed roles of two miRNAs in apple response to mannitol.Our results reveal candidate miRNAs and their associated mRNAs that could be targeted for improving drought tolerance in Malus species,thus providing a foundation for understanding the molecular networks involved in the response of apple trees to drought stress. | Chundong Niu Haiyan Li Lijuan Jiang Mingjia Yan Cuiying Li Dali Geng Yinpeng Xie Yan Yan Xiaoxia Shen Pengxiang Chen Jun Dong Fengwang Ma Qingmei Guan | 2019 | Horticulture Research2019，6，1： | 2 |
| 15 | The epidemiology and genetics of pituitary adenomas显示文摘 | Daly AF Tichomirowa MA Beckers A | 2009 | Best Pract Res Clin Endocrinol Metab2009，23，5： | 1 |
| 16 | The epidemiology and genetics of pituitary adenomas 显示文摘 | Daly AF Tichomirowa MA Beckers A | 2009 | Best Pract Res Clin Endocrinol Metab2009，23，5： | 1 |
| 17 | Toll- like receptor-4 is expressed in meningiomas and mediates the an- tiproliferative action of paclitaxel显示文摘 | Tichomirowa MA Theodoropoulou M Daly AF | 2008 | Int J Cancer2008，123，8： | 1 |
| 18 | High prevalence of AIP gene mutations following focused screening in young patients with sporadic pituitary macroadenomas显示文摘 | Tichomirowa MA Barlier A Daly AF | 2011 | Eur J Endocrinol2011，165，4： | 1 |
| 19 | The epidemiology and genetics of pituitary adenomas 显示文摘 | Daly AF Tichomirowa MA Beckers A | 2009 | Best Pract Res Clin Endocrinol Metab2009，23，5： | 1 |
| 20 | Cardiac surgery in patients with body mass index of 50 or greater 显示文摘 | Villavicencio MA Sundt TM3RD Daly RC | 2007 | Ann Thorac Surg2007，83，4： | 1 |
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