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| 1 | Geologic characteristics, controlling factors and hydrocarbon accumulation mechanisms of China’s Large Gas Provinces of low porosity and permeability显示文摘Based on the analysis of the geological characteristics and controlling factors, we analyzed the formation mechanism of different types of gas reservoirs. The main characteristics of gas provinces with low porosity and permeability are mainly as follows: large area, low abundance, small gas pools and large gas provinces; widely distributed excellent hydrocarbon source rocks with closely contacted source-reservoir-cap association; development mainly in large continental depressions or in paralic shallow-river delta systems; many kinds of traps coexisting in large areas, dominantly para-layered lithologic, digenetic and capillary pressure traps; double fluid flow mechanisms of Darcy flow and non-Darcy flow; complicated gas and water relations; and having the resource distribution of highly productive 'sweet spots', banding concentration, and macroscopically large areas integrated. The main controlling factors of large sandstone gas provinces with low porosity and permeability are stable dynamic backgrounds and gentle structural frameworks which control the extensive distribution of alternate (interbedded) sandstones and mudstones; weak hydropower of large gentle lake basins controlling the formation of discontinuous, low porosity and permeability reservoirs in shallow-water deltas; regionally differential diagenesis and no homogeneous digenetic facies controlling the development of favorable reservoirs and digenetic traps; and weak and dispersive reservoir-forming dynamic forces leading to the widely distributed small traps with low abundance. Low porosity and permeability gas provinces with different trap types have different formation mechanisms which include fluid diversion pressure difference interactive mechanism of lithologic-trap gas accumulations, separated differential collection mechanism of digenetic-trap gas accumulations, and the Non-Darcy flow mechanism of capillary-pressure gas accumulations. | ZOU CaiNeng TAO ShiZhen ZHANG XiangXiang HE DongBo ZHOU ChuanMin GAO XiaoHui | 2009 | Science China Earth Sciences2009，52，8： | 22 |
| 2 | Effects of Al_2O_3 Nano-additive on Performance of Micro-arc Oxidation Coatings Formed on AZ91D Mg Alloy显示文摘Ceramic coatings were prepared on AZ91 D Mg alloy by micro-arc oxidation(MAO) in aluminate electrolytes,with Al_2O_3 nano-additive suspending at different concentrations.Effects of nano-additive concentration on the structure,phase composition,hardness and anti-corrosion property of the MAO coatings were analyzed by scanning electron microscopy,X-ray diffraction,micro-hardness test and electrochemical method,respectively.The results revealed that Al_2O_3 nano-particles were mostly incorporated into ceramic coating chemically,transferred into MgAl_2O_4,rather than being trapped mechanically during MAO process.With the increase of Al_2O_3 concentration,the voltage-time response,content of MgAl_2O_4,hardness and anti-corrosion property increased.However,when the concentration varied from 10 g/L to 15 g/L,these behaviors and properties changed only a little.This result indicated that,after the concentration of Al_2O_3 nano-additive reaching 10 g/L,the incorporation of Al_2O_3 nano-particles turned into a saturation state,due to the complex process during MAO treatment.Therefore,10 g/L might be a proper concentration for MAO coating to incorporate Al_2O_3 nano-particles. | Yan Wang Dongbo Wei Jie Yu Shichun Di | 2014 | Journal of Materials Science & Technology2014，30，10： | 18 |
| 3 | Foreign Value-added in China's Manufactured Exports:Implications for China's Trade Imbalance显示文摘经济学家们最近对国内增值实际上在中国的出口被包括的 weighting 变得感兴趣。正式,外国、国内的内容的比例能被用非竞争的输入产量桌子计算垂直专门化份额识别。因为中国有处理出口的大份额,然而,把如此的一个方法用于中国盒子将导致大测量偏爱,它利用进口中介的一个不相称地高的百分比。这份报纸,能被直接采用为哪个在处理和非处理的贸易进口了中介的 2008 贸易数据借助于各种各样的贸易模式识别,提供一个简化方法估计在工业级的生产出口包括的外国 / 国内的增值的份额。这份报纸为平常的出口发现处理出口的中国的垂直专门化份额在 2008 是大约 56% ,与大约 10% 相比。它也发现处理的富有经验的快扩大出口的部门有外国内容的高得多的份额。因为处理,出口说明关于中国出口,占优势的贸易统计,它集中于粗野价值而非出口和进口的增值的一半,显然说大话双边的贸易不平衡,特别在中国和美国之间。 | Jun Zhang Dongbo Tang Yubo Zhan | 2012 | China & World Economy2012，20，1： | 16 |
| 4 | Fluvastatin prevents renal injury and expression of lactin-like exidized low-density lipoprotein receptor-1 in rabbits with hypercholesterolemia显示文摘Background Lipid abnormalities are often complicated by renal dysfunction. 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors (statins) are the first-line choice for lowering cholesterol levels. The present study was designed to investigate whether statins could prevent and invert the development of renal injury in cholesterol-fed rabbits and to find the possible mechanism of their effects by detecting gene and protein expression of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) in the renal artery.Methods Twenty-four male New Zealand white rabbits were divided into three groups: (1) control group, regular granules chow; (2) HC-diet group, granules chow with 1% cholesterol and 5% lard oil; and (3) fluvastatin group, 1% cholesterol and 5% lard oil diet plus fluvastatin (10 mg·kg -1 ·d -1 ) . After 16 weeks, serum total cholesterol (TC), low-density lipoprotein(LDL) and creatinine (Cr) levels were measured. Renal hemodynamics and function, mainly including glomerular filtration rate (GFR) in vivo were quantified using 99m Tc-DTPA single photon emission computed tomograph ( 99m Tc-DTPA SPECT). The thickness of the renal artery intima was quantitated in HE-stained segments by histomorphometry. Gene expression of LOX-1 in the renal artery was examined by semi-quantitative RT-PCR and its protein expression was evaluated by immunohistochemistry.Results High cholesterol diet induced hypercholesterolemia (HC) complicated by renal dysfunction with increased levels of serum lipid and Cr, decreased GFR and delayed excretion and extensively thickened renal arterial intima in the HC-diet group. Rabbits in the control group showed a minimal LOX-1 expression (mRNA and protein) in the endothelium and neointima of the renal artery. Intimal proliferation of the renal artery in the HC-diet group was associated with a marked increase of LOX-1 expression (protein and mRNA). Treatment with fluvastatin improved renal function, attenuated intimal proliferation of the renal artery and markedly decreased the enhanced LOX-1 expression in the endothelium and neointima of the renal artery in rabbits. Conclusions Fuvastatin treatment could prevent the development of renal injury in patients with HC and early atherosclerosis (AS). This beneficial effect might be mediated by its pleiotropic effects including a decrease in total cholesterol exposure level and prevention of LOX-1 expression in atherosclerotic arteries. | YUYong-hui WANGYi DONGBo SUNShu-zhen CHENYao MENGXiao-hui LIUZhong-zhi | 2005 | Chinese Medical Journal2005，，8： | 11 |
| 5 | Specific targeting of nasopharyngeal carcinoma cell line CNE1 by C225-conjugated ultrasmall superparamagnetic iron oxide particles with magnetic resonance imaging显示文摘临床的目标体积(CTV ) 的一个精确定义为在鼻咽的癌(NPC ) 的放射疗法的应用程序是必要的处理。指向对比代理人(C225-USPIO ) 的新奇表皮的生长因素受体(EGFR ) 被结合 ultrasmall superparamagnetic 设计有 cetuximab (C225 ) 的氧化铁(USPIO ) nanoparticles, non-invasively 定义肿瘤的 CTV。到 NPC 房间线 CNE1 的 C225-USPIO 的 immunobinding 活动被流动 cytometry 和 immunofluorescence 证实。在 CNE1 房间的 C225-USPIO 的时间依赖者累积用普鲁士的蓝染色被评估。指向的成为主观和 C225-USPIO 的 subcellular 本地化被传播电子显微镜证实。结果显示了明确地在 CNE1 房间的表面上绑在 EGFR 的那 C225-USPIO 并且被收起进房间。由 CNE1 房间的 C225-USPIO 的举起与时间显著地增加了,当时与人的 IgG-USPIO 相比。另外, 4.7 T 磁性的回声成像(MRI ) 随着时间的过去表明 C225-USPIO 有一个能力在 CNE1 房间积累,与在 MRI T2 加权的信号紧张的结果的显著减少。这些调查结果当一个 MRI 对比代理人和罐头被采用 non-invasively 与 EGFR overexpression 检测早阶段的 NPC,暗示 C225-USPIO 有潜力。这为与混合物在 vivo 实验开始提供足够的理论基础。 | Dongbo Liu Chunli Chen Guangyuan Hu Qi Mei Hong Qiu Guoxian Long Guoqing Hu | 2011 | Acta Biochimica et Biophysica Sinica2011，43，4： | 10 |
| 6 | Effects of Grazing Exclusion on Soil Carbon and Nitrogen Storage in Semi-arid Grassland in Inner Mongolia, China显示文摘The semi-arid grasslands in Inner Mongolia, China have been degraded by long-term grazing. A series of ecological restoration strategies have been implemented to improve grassland service. However, little is known about the effect of these ecological restoration practices on soil carbon and nitrogen storage. In this study, characteristics of vegetation and soil properties under continued grazing and exclusion of livestock for six years due to a nationwide conservation program—′Returning Grazing Lands to Grasslands(RGLG)′ were examined in semi-arid Hulun Buir grassland in Inner Mongolia, China. The results show that removal of grazing for six years resulted in a significant recovery in vegetation with higher above and below-ground biomass, but a lower soil bulk density and pH value. After six years of grazing exclusion, soil organic C and total N storage increased by 13.9% and 17.1%, respectively, which could be partly explained by decreased loss and increased input of C and N to soil. The effects of grazing exclusion on soil C and N concentration and storage primarily occurred in the upper soil depths. The results indicate that removal of grazing pressure within the RGLG program was an effective restoration approach to control grassland degradation in this region. However, more comprehensive studies are needed to evaluate the effectiveness of the RGLG program and to improve the management strategies for grassland restoration in this area. | WU Xing LI Zongshan FU Bojie LU Fei WANG Dongbo LIU Huifeng LIU Guohua | 2014 | Chinese Geographical Science2014，24，4： | 10 |
| 7 | Observations of the first meteorological rocket of the Meridian Space Weather Monitoring Project显示文摘On June 3,2010,the first meteorological rocket of the Meridian Space Weather Monitoring Project was successfully launched at the Hainan rocket launch site(19.5°N),China.This paper analyzes the vertical profiles of atmospheric temperature and wind recorded by the rocket and its supportive balloons,and investigates wind shear,gravity waves and atmospheric stability.In addition,we compare the vertical profiles of atmospheric temperature and wind with SABER/TIMED temperature measurements and the modeling results of MSIS00 and HWM07. | JIANG GuoYing XU JiYao SHI DongBo WEI Feng WANG LianZhong | 2011 | Chinese Science Bulletin2011，56，20： | 9 |
| 8 | 吴康衡教授治疗肾性血尿经验采撷显示文摘肾性血尿属中医'溺血'、'溲血'之范畴,是多种肾脏疾病常见临床表现之一。然由于病机复杂,涉及病种繁多,且经常反复,临床医生常感束手。本文记载并整理了全国名老中医吴康衡教授治疗该症的诊疗思路,治疗模式,以及用药特点。其中西结合,病证结合,常变结合的诊疗思路对临床医师颇有启发之处,故而录之以兹同仁参考。 | 张丰华 孙香娟 Weng Dongbo | 2018 | 四川中医2018，36，1： | 9 |
| 9 | Monovalent cation perm-selective membranes(MCPMs)'New developments and perspectives显示文摘As one of the most typical and promising membrane processes, electrodialysis(ED) technique plays a more and more significant role in industrial separation. Especially, the separation of monovalent cations and multivalent cations is currently a hot topic, which is not only desirable for many industries but also challenging for academic explorations. The main aim of the present contribution is to view the advances of a wide variety of monovalent cation perm-selective membranes(MCPMs) and their preparation technologies including(1) covalent crosslinking,(2) surface modification,(3) polymer blending,(4) electrospinning,(5) nanofiltration alike membrane,and(6) organic–inorganic hybrid. The relevant advantages and disadvantages with respect to some specific cases have been discussed and compared in detail. Furthermore, we elaborately discuss the opportunities and challenges of MCPMs, the fabricating strategies to take and the future perspectives. | Liang Ge Bin Wu Dongbo Yu Abhishek N.Mondal Linxiao Hou Noor U1 Afsar Qiuhua Li Tingting Xu Jibin Miao Tongwen Xu | 2017 | Chinese Journal of Chemical Engineering2017，25，11： | 8 |
| 10 | A critical review on the application of biochar in environmental pollution remediation:Role of persistent free radicals(PFRs)显示文摘Biochar as an emerging carbonaceous material has exhibited a great potential in environmental application for its perfect adsorption ability.However,there are abundant persistent free radicals(PFRs)in biochar,so the direct and indirect PFRs-mediated removal of organic and inorganic contaminants by biochar was widely reported.In order to comprehend deeply the formation of PFRs in biochar and their interactions with contaminants,this paper reviews the formation mechanisms of PFRs in biochar and the PFRs-mediated environmental applications of biochar in recent years.Finally,future challenges in this field are also proposed.This review provides a more comprehensive understanding on the emerging applications of biochar from the viewpoint of the catalytic role of PFRs. | Kun Luo Ya Pang Dongbo Wang Xue Li Liping Wang Min Lei Qi Huang Qi Yang | 2021 | Journal of Environmental Sciences2021，33，10： | 8 |
| 11 | Quantitative traits correlative analysis and growth comparison among different populations of bay scallop,Argopecten irradians显示文摘The shell traits and weight traits are measured in cultured populations of bay scallop, Argopecten irradians. The results of regression analysis show that the regression relationships for all the traits are significant (P<0.01). The correlative coefficients between body weight, as well as tissue weight with shell length, shell height and shell width are significant (P < 0.05). But the correlative coefficients between the anterior and posterior auricle length with body weight as well as tissue weight are not significant (P>0.05). The multiple regression equation is obtained to estimate live body weight and tissue weight. The above traits except anterior and posterior auricle length are used for the growth and production comparison among three cultured populations, Duncan's new multiple range procedure analysis shows that all the traits in the Lingshuiqiao (LSQ) population are much more significant than those of the other two populations (P<0.01), and there is no significant diffei'ence between the Qipanmo (QPM) and Dalijia (DLJ) populations in all traits (P>0.05). The results indicate that the LSQ population has a higher growth rate and is expected to be more productive than the other two populations. | LIUBaozhong LIANGYubo LIUXiaolin DONGBo XIANGJianhai | 2004 | Acta Oceanologica Sinica2004，23，3： | 7 |
| 12 | G protein-coupled receptor 183 facilitates endothelial-to- hematopoietic transition via Notch1 inhibition显示文摘 | Panpan Zhang Qiuping He Dongbo Chen Weixiao Liu Lu Wang Chunxia Zhang Dongyuan Ma Wei Li Bing Liu Feng Liu | 2015 | Cell Research2015，25，10： | 7 |
| 13 | Characteristics of the phytoplankton community and bioaccumulation of heavy metals during algal blooms in Xiangjiang River (Hunan,China)显示文摘The frequency of algal blooms has increased in the mid and downstream reaches of the Xiangjiang River (Hunan,China),one of the most heavily polluted rivers in China.We identified the bloom-forming species in a bloom that occurred mid-late September 2010.In addition,we determined the extent of metal bioaccumulation in the algae and measured the toxicity of the algae using a mouse bioassay.Water samples were collected at upstream (Yongzhou),midstream (Hengyang),and downstream (Zhuzhou,Xiangtan,and Changsha) sites.The dominant species was Aulacoseira granulata,formerly known as Melosira granulata.The heaviest bloom occurred at Xiangtan and Changsha,where the number of A.granulata peaked at 1.3×10 5 filaments L 1 and chlorophyll a at 0.04 mg L 1.Concentrations of Al,Fe,and Mn were 4.4 10 3,768.4,and 138.7 mg kg 1 dry weight in the phytoplankton.The bioaccumulation factor was 4.0×10 5,7.7×10 5,and 3.2×10 3,respectively.The heavy metal Pb had the greatest tendency to bioaccumulate among the highly toxic heavy metals,with a concentration of 19.2 mg kg 1 dry weight and bioaccumulation factor of 9.6×10 3.The mouse bioassay suggested the bloom was toxic.The LD 50 was 384 mg kg 1 and all surviving mice lost weight during the first 72 h after exposure.Our results demonstrate that blooms of A.granulata in rivers contaminated with heavy metals pose a threat to freshwater ecosystems and human health.Thus,measures should be taken to control eutrophication and heavy metal pollution in such rivers. | LI Jie PENG FuLi DING DongBo ZHANG ShuBing LI DeLiang ZHANG Ting | 2011 | Science China(Life Sciences)2011，54，10： | 6 |
| 14 | Enhancement of precursor amino acid supplies for improving bacitracin production by activation of branched chain amino acid transporter BrnQ and deletion of its regulator gene lrp in Bacillus licheniformis显示文摘Bacitracin,a new type of cyclic peptide antibiotic,is widely used as the feed additive in feed industry.Branched chain amino acids(BCAAs)are the key precursors for bacitracin synthesis.In this research,soybean meal was served as the raw material to supply precursor amino acids for bacitracin synthesis,and enhanced production of bacitracin was attempted by engineering BCAA transporter BrnQ and its regulator Lrp in the bacitracin industrial production strain Bacillus licheniformis DW2.Firstly,our results confirmed that Lrp negatively affected bacitracin synthesis in DW2,and deletion of lrp improved intracellular BCAA accumulations,as well as the expression level of BCAA transporter BrnQ,which further led to a 14.71%increase of bacitracin yield,compared with that of DW2.On the contrary,overexpression of Lrp decreased bacitracin yield by 12.28%.Secondly,it was suggested that BrnQ acted as a BCAA importer in DW2,and overexpression of BrnQ enhanced the intracellular BCAA accumulations and 10.43%of bacitracin yield.While,the bacitracin yield decreased by 18.27%in the brnQ deletion strain DW2△brnQ.Finally,BrnQ was further overexpressed in lrp deletion strain DW2△lrp,and bacitracin yield produced by the final strain DW2△lrp::BrnQ was 965.34 U/mL,increased by 22.42%compared with that of DW2(788.48 U/mL).Collectively,this research confirmed that Lrp affected bacitracin synthesis via regulating the expression of BCAA transporter BrnQ and BCAA distributions,and provided a promising strain for industrial production of bacitracin. | Jiang Zhu Dongbo Cai Haixia Xu Ziwei Liu Bowen Zhang Fei Wu Junhui Li Shouwen Chen | 2018 | Synthetic and Systems Biotechnology2018，3，4： | 6 |
| 15 | Machining fixture for adaptive CNC machining process of near-net-shaped jet engine blade显示文摘Low stiffness and positioning problems are difficulties and challenges in the precise machining of near-net-shaped blades.This paper aims to achieve high accuracy in manufacturing by fixture-and deformation-control in the adaptive CNC machining process.Adaptive CNC machining technology is first analyzed,and new fixture-evaluation criteria and methods to evaluate the adaptive CNC machining process fixture design are built.Second,a machining fixture is designed and manufactured after analyzing its positioning scheme,clamping scheme,materials(PEEK-GF30),and structure characteristics.Finally,the designed fixture is analyzed by FEA and experimentally verified by a cutting experiment.The results show that the deformation of the blade is an overall rigid-body displacement,the main deformation of the blade-fixture system occurs on the four clamping heads,and this fixture can effectively protect the blade from local deformation.The proposed clamping-sequence method reliably and effectively controls the local maximum deformation of the blade.The system stiffness is increased by 20 Hz,with each clamping force increased by 200 N.Both high-and low-frequency displacement in roughing milling or finishing milling are acceptable relative to the accuracy demand of blade machining.This fixture and an adaptive CNC machining process can achieve high accuracy in blade manufacturing. | Dongbo WU Hui WANG Jinsong PENG Kaiyao ZHANG Jie YU Xuesong ZHENG Yunhai CHEN | 2020 | Chinese Journal of Aeronautics2020，33，4： | 6 |
| 16 | Smart Substation Integration Technology and Its Application in Distribution Power Grid显示文摘At present,smart substations use the IEC61850 standard based on the architectural framework of three layers and two networks to realize information digitization and advanced applications.Although the smart substation offers many improvements in design,equipment manufacturing,and construction,the intelligent devices used in smart substations are costly,and are also difficult to maintain since they are dispersed within a single unit.Functionality optimization and device integration,thus,have become important issues in smart substation development.This paper presents an integrated solution and implementation process for a smart substation system.In the process layer,an integrated intelligence component is developed that functions both as an intelligent terminal and a merging unit.In the bay layer,an integrated station-area protection measurement and control master device is designed to achieve such functions as protection,monitoring,control,fault recorder,and power quality monitoring.Finally,in the station control layer,an integrated information platform is established to bring together various system functions and to promote interactive sharing.Integration technology improves the economy and practicality of the smart substation,especially in a distribution power grid. | Qipeng Song Wanxing Sheng Lingfeng Kou Dongbo Zhao Ziping Wu Hengfu Fang Xiaolong Zhao | 2016 | CSEE Journal of Power and Energy Systems2016，2，4： | 6 |
| 17 | A New Species of the Genus Tylototriton (Urodela: Salamandridae) from Northeastern Hunan Province, China显示文摘We describe a new species from the family Salamandridae from northeastern Hunan Province of China based on morphological and molecular genetic evidences.The new species,Tylototriton liuyangensis,is a member of the T.asperrimus group,and shares a number of similarities with T.wenxianensis and T.broadoridgus.The dorsal surface of the new Tylototriton species is completely black,with nodule-like warts distributed evenly along the lateral margin of dorsal body,and there is no transverse striae between the warts.There are several diagnostic characters of the new species that differ from those of T.wenxianensis and T.broadoridgus as follows:(1)the interorbital space(IOS)is comparatively wide;(2)there is a bony ridge present on the dorsal head surface,running along the upper eyelids;(3)the space between axilla and groin(AGS)is comparatively large,with the mean male AGS approximately equal to 50%of the snout-vent length(SVL)and the mean female AGS being 53%of SVL;and(4)there is a small genital armature on the anterior angle of labial folds present inside the anal fissure of adult males during the breeding season.The result from the molecular phylogenetic analysis of the genus Tylototriton(including the type specimen of the new species)based on the complete DNA sequence of the mitochondrial ND2 gene(1035 bp)indicated that the new species was similar to T.wenxianensis,T.dabienicus,and T.broadoridgus,but formed an independent clade.This result was consistent with the morphological analysis above,supporting that the Liuyang Population represented a distinct species. | Daode YANG Jianping JIANG Youhui SHEN Dongbo FEI | 2014 | Asian Herpetological Research2014，5，1： | 5 |
| 18 | Numerical simulation for erosion effects of three-phase flow containing sulfur particles on elbows in high sour gas fields显示文摘Sulfur particles carried by high-speed flow impact pipelines,which may cause equipment malfunctions and even failure.This paper investigates the scouring effect of mist gas containing sulfur particles on elbows in highly sour gas fields.The multiphase-flow hydrodynamic model of the 90elbow was established by using the computational fluid dynamics(CFD)method.The scouring effects of the gasliquid mist fluid with the water-liquid fraction of 20%and particles with the diameter of 0.01 e0.05mm on elbows were explored within the flow velocity range of 0e20 m/s.In addition,the influences of secondary collision,mean curvature radius to diameter(R/D)ratio,inertial force,drag force,and Stokes number on trajectories of sulfur particles were studied.Moreover,the influences of hydrodynamic parameters of multiphase flow on corrosion inhibitor film were analyzed with the wall shear stress as the reference value.Serious erosion mainly occurred in the extrados of elbow as well as the junction between downstream pipeline and the intrados of elbow,the maximum erosion area occurs at 61.9.When the incident position of the particle was far away from the top of the inlet plane,the probability of secondary collision became smaller.Furthermore,the erosion rate decreased with the rise in the R/D radio.The maximum erosion rate of elbow increased with the increase in the Stoke number.The maximum erosion rate reached 0.428 mm/a at 0.05mm particle diameter and 20 m/s fluid velocity.The wall shear stress increased with the increase in fluid velocity and mass flow rate of particle,the fitting function of the wall shear stress curve was the Fourier type.The results indicated that highvelocity particles had a serious erosion effect on elbows and affected the stability of the corrosion inhibitor film.The erosion effect could be retarded by controlling the velocity and diameter of particles.The results provided technical supports for the safe production in highly sour gas fields. | Enbo Zhang Dezhi Zeng Hongjun Zhu Shuanggui Li Dongbo Chen Jie Li Yanyan Ding Gang Tian | 2018 | Petroleum2018，4，2： | 5 |
| 19 | LRP6 downregulation promotes cardiomyocyte proliferation and heart regeneration显示文摘The adult mammalian heart is thought to be a terminally differentiated organ given the postmitotic nature of cardiomyocytes.Consequently,the potential for cardiac repair through cardiomyocyte proliferation is extremely limited.Low-density lipoprotein receptor-related protein 6(LRP6)is a Wnt co-receptor that is required for embryonic heart development.In this study we investigated the role of LRP6 in heart repair through regulation of cardiomyocyte proliferation.Lrp6 deficiency increased cardiomyocyte cell cycle activity in neonatal,juvenile and adult mice.Cardiomyocyte-specific deletion of Lrp6 in the mouse heart induced a robust regenerative response after myocardial infarction(MI),led to reduced MI area and improvement in left ventricular systolic function.In vivo genetic lineage tracing revealed that the newly formed cardiomyocytes in Lrp6-deficient mouse hearts after MI were mainly derived from resident cardiomyocytes.Furthermore,we found that the pro-proliferative effect of Lrp6 deficiency was mediated by the ING5/P21 signaling pathway.Gene therapy using the adeno-associated virus(AAV)9 miRNAi-Lrp6 construct promoted the repair of heart injury in mice.Lrp6 deficiency also induced the proliferation of human induced pluripotent stem cell-derived cardiomyocytes(iPSC-CMs).Our study identifies LRP6 as a critical regulator of cardiomyocyte proliferation,which may lead to the development of a novel molecular strategy to promote myocardial regeneration and repair. | Yahan Wu Liping Zhou Hongyu Liu Ran Duan Huixing Zhou Fulei Zhang Xiaoyu He Dongbo Lu Ke Xiong Maolin Xiong Jinzhu Zhuang Yi Liu Li Li Dandan Liang Yi-Han Chen | 2021 | Cell Research2021，31，4： | 5 |
| 20 | miR-155 aggravates impaired autophagy of pancreatic acinar cells through targeting Rictor显示文摘The aim of this study was to investigate the role and mechanism of miR-155 in regulating autophagy in a caerulein-induced acute pancreatitis(AP)cellular model.GFP-LC3 immunofluo-rescence assay was performed to detect autophagy vesicle formation in pancreatic acinar cell line AR42J.AR42J cells were transfected with miR-155 mimic,inhibitor,and corresponding controls to explore the effect of miR-155 on autophagy.The protein levels of LC3-I,LC3-11,Beclin-1,and p62 were analyzed by western blot analysis.Dual-luciferase reporter assay was performed to verify the interaction between miR-155 and Rictor(RPTOR independent companion of MTOR complex 2).The results showed that caerulein treatment induced impaired autophagy as evi-denced by an increase in the accumulation of p62 together with LC3-11 in AR42J cells,accompanied by miR-155 upregulation.Furthermore,miR-155 overexpression aggravated,whereas miR-155 silencing reduced the caerulein-induced impairment of autophagy.Mechanistically,Rictor was confirmed to be a direct target of miR-155,which could rescue the miR-155 overexpression-mediated aggravation of im paired autophagy.Collectively,these findings indicate that miR-155 aggravates impaired autophagy in caerulein-treated pancreatic acinar cells by targeting Rictor. | Xueming Zhang Jiangtao Chu Haijun Sun Dali Zhao Biao Ma Dongbo Xue Weihui Zhang Zhituo Li | 2020 | Acta Biochimica et Biophysica Sinica2020，52，2： | 4 |
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