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| 1 | Wx^lv、the Ancestral Allele of Rice Waxy Gene显示文摘In rice grains,the Waxy (Wx) gene is responsible for the synthesis of amylose,the most important determinant for eating and cooking quality.The effects of several Wx alleles on amylose content and the taste of cooked rice have been elucidated.However,the relationship between artificial selection and the evolution of various Wx alleles as well as their distribution remain unclear.Here we report the identification of an ancestral allele,Wx^lv,which dramatically affects the mouthfeel of rice grains by modulating the size of amylose molecules.We demonstrated that WF originated directly from wild rice,and the three major Wx alleles in cultivated rice (Wx^b,Wx^a,and Wx^in) differentiated after the substitution of one base pair at the functional sites.These data indicate that the Wx^lv allele played an important role in artificial selection and domestication.The findings also shed light on the evolution of various Wx alleles,which have greatly contributed to improving the eating and cooking quality of rice. | Changquan Zhang Jihui Zhu Shengjie Chen Xiaolei Fan Qianfeng Li Yan Lu Min Wang Hengxiu Yu Chuandeng Yi Shuzhu Tang Minghong Gu Qiaoquan Liu | 2019 | Molecular Plant2019，12，8： | 22 |
| 2 | A rare Waxy allele coordinately improves rice eating and cooking quality and grain transparency显示文摘In rice(Oryza sativa), amylose content(AC) is the major factor that determines eating and cooking quality(ECQ). The diversity in AC is largely attributed to natural allelic variation at the Waxy(Wx)locus. Here we identified a rare Wx allele, Wx^(mw) ,which combines a favorable AC, improved ECQ and grain transparency. Based on a phylogenetic analysis of Wx genomic sequences from 370 rice accessions, we speculated that Wx^(mw) may have derived from recombination between two important natural Wx alleles, Wx^(in) and Wx^(b). We validated the effects of Wx^(mw) on rice grain quality using both transgenic lines and near-isogenic lines(NILs). When introgressed into the japonica Nipponbare(NIP) background, Wx^(mw) resulted in a moderate AC that was intermediate between that of NILs carrying the Wx^(b)allele and NILs with the Wx^(mp) allele. Notably, mature grains of NILs fixed for Wx^(mw) had an improved transparent endosperm relative to soft rice. Further, we introduced Wx^(mw) into a high-yielding japonica cultivar via molecular marker-assisted selection: the introgressed lines exhibited clear improvements in ECQ and endosperm transparency. Our results suggest that Wx^(mw) is a promising allele to improve grain quality, especially ECQ and grain transparency of high-yielding japonica cultivars, in rice breeding programs. | Changquan Zhang Yong Yang Shengjie Chen Xueju Liu Jihui Zhu Lihui Zhou Yan Lu Qianfeng Li Xiaolei Fan Shuzhu Tang Minghong Gu Qiaoquan Liu | 2021 | Journal of Integrative Plant Biology2021，63，5： | 18 |
| 3 | Modeling effects of temperature and precipitation on carbon characteristics and GHGs emissions in Abies fabric forest of subalpine显示文摘Abies fabric forest in the eastern slope of Gongga mountain is one type of subalpine dark coniferous forests of southwestern China. It is located on the southeastern edge of the Qinghai-Tibet plateau and is sensitive to climatic changes. A process-oriented biogeochemical model, Forest-DNDC, was applied to simulate the effects of climatic factors, temperature and precipitation changes on carbon characteristics, and greenhouse gases (GHGs) emissions in A. fabric forest. Validation indicated that the Forest-DNDC could be used to predict carbon characteristics and GHGs emissions with reasonable accuracy. The model simulated carbon fluxes, soil carbon dynamics, soil CO2, N2O, and NO emissions with the changes of temperature and precipitation conditions. The results showed that with variation in the baseline temperature from -2℃to +2℃, the gross primary production (GPP) and soil organic carbon (SOC) increased, and the net primary production (NPP) and net ecosystem production (NEP) decreased because of higher respiration rate. With increasing baseline precipitation the GPP and NPP increased slightly, and the NEP and SOC showed decreasing trend. Soil CO2 emissions increased with the increase of temperature, and CO2 emissions changed little with increased baseline precipitation. With increased temperature and decreased baseline temperature, the total annual soil N2O emissions increased. With the variation of baseline temperature from -2℃to +2℃, the total annual soil NO emissions increased. The total annual N2O and NO emissions showed increasing trends with the increase of precipitation. The biogeochemical simulation of the typical forest indicated that temperature changes strongly affected carbon fluxes, soil carbon dynamics, and soil GHGs emissions. The precipitation was not a principal factor affecting carbon fluxes, soil carbon dynamics, and soil CO2 emissions, but changes in precipitation could exert strong effect on soil N2O and NO emissions. | LU Xuyang CHENG Genwei XIAO Feipeng FAN Jihui | 2008 | Journal of Environmental Sciences2008，20，3： | 7 |
| 4 | Distribution Characteristics and Influencing Factors of Geological Hazards in Tibet显示文摘Tibet is one of the areas with most serious geological hazards in China, and the distribution of disasters has obvious local characteristics. Tibet can be classified as three parts through zoning the danger degree, the mountain canyon high danger zone of east and southeast Tibet, the plateau mountain lake basin and valley middle danger zone of south Tibet , and the Plateau Mountain lake basin low danger zone of south Tibet. This paper takes the debris flow, collapse, landslide as the key points to analyze the distribution characteristics of geological hazards, and analyze the factors which influence the distribution of geological hazards, such as terrain landform, formation lithology, geologic structure pattern, precipitation, earthquake, human activity and so on. finally, as a conclusion: in whole Tibet, the geological hazards are more in southeast than in northwest, more in mountainous area which in the edge of plateau and river valley than in the interior of plateau and lake basin. And most hazards distribute in the regions where human activity is stronger than in other regions, for example towns or strips along the highway. | FAN Jihui WU Caiyan Cheng Genwei | 2006 | Wuhan University Journal of Natural Sciences2006，11，4： | 5 |
| 5 | Dynamics of Above-and Below-ground Biomass and C,N,P Accumulation in the Alpine Steppe of Northern Tibet显示文摘The temporal dynamics of the biomass,as well as the carbon (C),nitrogen (N),phosphorus (P) concentrations and accumulation contents,in above-and below-ground vegetation components were determined in the alpine steppe vegetation of Northern Tibet during the growing season of 2010.The highest levels of total biomass (311.68 g m-2),total C (115.95 g m-2),total N (2.60 g m-2),and total P (0.90 g m-2) accumulation contents were obtained in August in 2010.Further,biomass and nutrient stocks in the below-ground components were higher than those of the above-ground components.The dominant species viz.,Stipa purpurea and Carex moorcrofti had lower biomass and C,N,P accumulations than the companion species which including Oxytropis.spp.,Artemisia capillaris Thunb.,Aster tataricus L.,and so on. | LU Xuyang YAN Yan FAN Jihui CAO Yingzi WANG Xiaodan | 2011 | Journal of Mountain Science2011，8，6： | 5 |
| 6 | Outburst Risk of Barrier Lakes in Sichuan,China显示文摘34 barrier lakes induced by earthquake have been formed by wedged debris on the river channels after a massive earthquake happening on May 12 in Sichuan, China. Among them, the Tangjiashan Barrier Lake is the largest one. It faces very urgent risk of dam breaking when water level reaches the top and begins overflow in case of storm rainfalls and continually aftershocks, threatening already devastated cities and villages with about 1.5 million people downstream. The outburst of a similar barrier lake occurred in the Minjiang River in 1933, causing a catastrophic flood. Risk analysis indicates that not all barrier lakes are highly dangerous. Only those lakes with very high dams and water to be filled up in short period need to be dealt with immediately. | CHENG Genwei WANG Xiaodan HE Xiubin FAN Jihui FAN Jianrong | 2008 | Journal of Mountain Science2008，5，3： | 4 |
| 7 | Low-temperature growth and optical properties of Ce-doped ZnO nanorods显示文摘 | Jinghai Yang Ming Gao Yongjun Zhang Lili Yang Jihui Lang Dandan Wang Huilian Liu Yanqing Liu Yaxin Wang Hougang Fan | 2008 | Applied Surface Science2008，255，： | 1 |
| 8 | ENO1 expression and Erk phosphorylation in PDAC and their effects on tumor cell apoptosis in a hypoxic microenvironment显示文摘Objective: Hypoxia is an important feature of pancreatic ductal adenocarcinoma(PDAC). Previously, we found that hypoxia promotes ENO1 expression and PDAC invasion. However, the underlying molecular mechanism was remains unclear.Methods: The relationship between ENO1 expression and clinicopathological characteristics was analyzed in 84 patients with PADC. The effects of CoCl2-induced hypoxia and ENO1 downregulation on the apoptosis, invasion, and proliferation of PDAC cells were evaluated in vitro and in vivo. Hypoxia-and ENO1-induced gene expression was analyzed by transcriptomic sequencing.Results: The prognosis of PDAC with high ENO1 expression was poor(P < 0.05). High ENO1 expression was closely associated with histological differentiation and tumor invasion in 84 PDAC cases(P < 0.05). Hypoxia increased ENO1 expression in PDAC and promoted its migration and invasion. Apoptotic cells and the apoptosis marker caspase-3 in the CoCl_(2)-treated ENO1-sh group were significantly elevated(P < 0.05). Transcriptomic sequencing indicated that CoCl_(2)-induced PDAC cells initiated MAPK signaling. Under hypoxic conditions, PDAC cells upregulated ENO1 expression, thereby accelerating ERK phosphorylation and inhibiting apoptosis(P < 0.05). Consistent results were also observed in a PDAC-bearing mouse hindlimb ischemia model.Conclusions: Hypoxia-induced ENO1 expression promotes ERK phosphorylation and inhibits apoptosis, thus leading to PDAC survival and invasion. These results suggest that ENO1 is a potential therapeutic target for PDAC. | Huizhi Sun Jing Mo Runfen Cheng Fan Li Yue Li Yuhong Guo Yanlei Li Yanhui Zhang Xiaoyu Bai Yalei Wang Xueyi Dong Danfang Zhang Jihui Hao | 2022 | Cancer Biology & Medicine2022，19，11： | 0 |
| 9 | Determining Critical Support Discharge of a Riverhead and River Network Analysis: Case Studies of Lhasa River and Nyangqu River显示文摘A riverhead is the demarcation point of continuous water channel and seasonal channel, which is characterized by a critical flow that can support a continuous water body. In this study, the critical support discharge(CSD) is defined as the critical steady flows required to form the origin of a stream. The CSD is used as the criterion to determine the beginning of the riverhead, which can be controlled by hydro-climate factors(e.g., annual precipitation, annual evaporation, or minimum stream flow in arid season). The CSD has a close correlation with the critical support/source area(CSA) that largely affects the density of the river network and the division of sub-watersheds. In general, river density may vary with regional meteorological and hydrological conditions that have to be considered in the analysis. In this paper, a new model referring to the relationship of CSA and CSD is proposed, which is based on the physical mechanism for the origin of riverheads. The feasibility of the model was verified using two watersheds(Duilongqu Basin of the Lhasa River and Beishuiqu Basin of the Nyangqu River) in Tibet Autonomous Region to calculate the CSA and extract river networks. A series of CSAs based on different CSDs in derived equation were tested by comparing the extracted river networks with the reference network obtained from a digitized map of river network at large scales. Comparison results of river networks derived from digital elevation model with real ones indicate that the CSD(equal to criterion of flow quantity(Q_c)) are 0.0028 m^3/s in Duilongqu and 0.0085 m^3/s in Beishuiqu. Results show that the Q_c can vary with hydro-climate conditions. The Q_c is high in humid region and low in arid region, and the optimal Q_c of 0.0085 m^3/s in Beishuiqu Basin(humid region) is higher than 0.0028 m^3/s in Duilongqu Basin(semi-arid region). The suggested method provides a new application approach that can be used to determine the Q_c of a riverhead in complex geographical regions, which can also reflect the effect of hydro-climate change on rivers supply in different regions. | SHA Yukun LI Weipeng FAN Jihui CHENG Genwei | 2016 | Chinese Geographical Science2016，26，4： | 0 |
| 10 | Driving force of biomolecular liquid–liquid phase separation probed by nuclear magnetic resonance spectroscopy显示文摘The assembly of biomolecular condensates is driven by liquid–liquid phase separation. To understand the structure and functions of these condensates, it is essential to characterize the underlying driving forces, e.g., protein–protein and protein–RNA interactions. As both structured and low-complexity domains are involved in the phase separation process, NMR is probably the only technique that can be used to depict the binding topology and interaction modes for the structured and nonstructured domains simultaneously. Atomic-resolution analysis for the intramolecular and intermolecular interactions between any pair of components sheds light on the mechanism for phase separation and biomolecular condensate assembly and disassembly. Herein, we describe the procedures used for the most extensively employed NMR techniques to characterize key interactions for biomolecular phase separation. | Hanyu Zhang Weiwei Fan Gilbert Nshogoza Yaqian Liu Jia Gao Jihui Wu Yunyu Shi Xiaoming Tu Jiahai Zhang Ke Ruan | 2022 | Biophysics Reports2022，8，2： | 0 |
| 11 | Tuning porosity of coal-derived activated carbons for CO_(2) adsorption显示文摘A simple method was developed to tune the porosity of coal-derived activated carbons,which provided a model adsorbent system to investigate the volumetric CO_(2)adsorption performance.Specifically,the method involved the variation of the activation temperature in a K2CO3 induced chemical activation process which could yield activated carbons with defined microporous(<2 nm,including ultra-microporous<1 nm)and meso-microporous structures.CO_(2)adsorption isotherms revealed that the microporous activated carbon has the highest measured CO_(2)adsorption capacity(6.0 mmol∙g^(–1)at 0℃and 4.1 mmol∙g^(-1)at 25℃),whilst ultra-microporous activated carbon with a high packing density exhibited the highest normalized capacity with respect to packing volume(1.8 mmol∙cm−3 at 0℃and 1.3 mmol∙cm^(–3)at 25℃),which is significant.Both experimental correlation analysis and molecular dynamics simulation demonstrated that(i)volumetric CO_(2)adsorption capacity is directly proportional to the ultra-micropore volume,and(ii)an increase in micropore sizes is beneficial to improve the volumetric capacity,but may lead a low CO_(2)adsorption density and thus low pore space utilization efficiency.The adsorption experiments on the activated carbons established the criterion for designing CO_(2)adsorbents with high volumetric adsorption capacity. | Zhipeng Qie Lijie Wang Fei Sun Huan Xiang Hua Wang Jihui Gao Guangbo Zhao Xiaolei Fan | 2022 | Frontiers of Chemical Science and Engineering2022，16，9： | 0 |
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