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| 1 | ACBD3 is required for FAPP2 transferring glucosylceramide through maintaining the Golgi integrity显示文摘Glycosphingolipid (GSL) metabolism is involved in various physiological processes, including all major cell signaling pathways, and its dysregulation is linked to some diseases. The four-phosphate adaptor protein FAPP2-mediated glucosylceramide (GlcCer) transport for complex GSL synthesis has been studied extensively. However, the molecular machinery of FAPP2 as a GlcCer-transferring protein remains poorly defined. Here, we identify a Golgi-resident protein, acyl-coenzyme A binding domain containing 3 (ACBD3), as an interacting partner of FAPP2. We find that ACBD3 knockdown leads to dramatic Golgi fragmentation, which subsequently causes FAPP2 dispersal throughout the cytoplasm and a decreased localization at trans-Golgi network. The further quantitative Upidomic analysis indicates that ACBD3 knockdown triggers abnormal sphingolipid metabolism. Interestingly, the expression of siRNA-resistant full-length ACBD3 can rescue these defects caused by ACBD3 knockdown. These data reveal critical roles for ACBD3 in maintaining the integrity of Golgi morphology and cellular sphingolipid homeostasis and establish the importance of the integrated Golgi complex for the transfer of GlcCer and complex GSL synthesis. | Jing Liao Yuxiang Guan Wei Chen Can Shi Dongdong Yao Fengsong Wang Sin Man Lam Guanghou Shui Xinwang Cao | 2019 | Journal of Molecular Cell Biology2019，11，2： | 3 |
| 2 | Molecular cloning and expression analysis of Crustin-like gene from Chinese shrimp Fenneropenaeus chinensis显示文摘A new member of antimicrobial protein genes of the Crustin family was cloned from haemocytes of the Chinese shrimp Fenneropenaeus chinensis by 3′and 5′ RACE. The full-length cDNA of Crustin-like gene contains a 390 bp open reading frame, encoding 130 amino acids. The deduced peptide contains a putative signal peptide of 17 amino acids and mature peptide of 113 amino acids. The molecular mass of the deduced mature peptide is 12.3 ku. It is highly cationic with a theoretical isoelectric point of 8.5. The deduced amino acids sequence of this Crustin showed high homology with those of Penaeus (Litopenaeus) setferus. Northern blotting showed that the cloned Crustin gene was mainly expressed in haemocytes, gill, intestine, and RNA in situ hybridization indicated that the Crustin gene was constitutively expressed exclusively in haemocytes of these tissues. Capillary electrophoresis RT-PCR analysis showed that Crustin was up-regulated dramatically from 12 to 48 h after a brief decrease of mRNA during first 6 h in response to microbe infection. The level of Crustin mRNA began to restore at 72 h post-challenge. This indicated that Crustin gene might play an important role when shrimps are infected by bacterial pathogen. | LIU Fengsong LI Fuhua XIANG Jianhai DONG Bo LIU Yichen ZHANG Xiaojun ZHANG Liusuo | 2008 | Acta Oceanologica Sinica2008，27，2： | 2 |
| 3 | Use of surface coatings with natamycin to improve the storability of Hami melon at ambient temperature显示文摘 | Fengsong Cong Yungui Zhang Wenyan Dong | 2007 | Postharvest Biology and Technology2007，46，： | 1 |
| 4 | Simultaneous Determination of Dopamine and Uric Acid at 2-Amino-5-mercapto-[1, 3, 4]triazole Self-assembled Monolayers Gold Electrode显示文摘A newly synthesized reagent 2-amino-5-mercapto-[1, 3, 4]triazole (MATZ) has been usedto fabricate self-assembled monolayers (SAMs) on gold electrode for the first time. The SAMselectrode was characterized by electrochemical methods and scanning electronic microscopy (SEM),the SAMs electrode can be used to determinate dopamine (DA) and uric acid (UA) simultaneouslywith a detection limit of 8×10-7 mol/L for DA and 1×10-6 mol/L for UA respectively. The SAMscan also be used to detect the contents of DA and UA in synthetic urine sample with satisfactoryresults. | ChuanYinLIU LiZhuYANG FengSONG LingYanJIANG GuangHanLU | 2005 | Chinese Chemical Letters2005，16，2： | 1 |
| 5 | Dysbindin promotes progression of pancreatic Juctal adenocarcinoma via direct activation of PI3显示文摘 | Cheng Fang Xin Guo Xing Lv Ruozhe Yin Xiaohui Lv Fengsong Wang Jun Zhao Quan Bai Xuebiao Yao Yong Chen | 2017 | Journal of Molecular Cell Biology2017，9，6： | 1 |
| 6 | Use of surface coatings with natamycin to improve the storability of Hami melon at ambient temperature显示文摘 | CONG Fengsong ZHANG Yungui DONG Wenyan | | 0，，01： | 1 |
| 7 | Separating algorithm for cell image based on boundary2stripped 显示文摘 | Liu Xiangbin Zou Beiji Hu Fengsong | 2002 | Journal of Image and Graphics ( A)2002，7，3： | 1 |
| 8 | Use of sur- face coating with natamycin to improve the storability of Hami melon at ambient temperature显示文摘 | Cong Fengsong Zhang Yuigui Dong Wenyan | 2007 | Postharvest Biolo- gy and Technology2007，46，： | 1 |
| 9 | The study of human face recognition based curvelet transform and 2DPCA显示文摘 | Ma Hui Hu Fengsong | 2010 | ICISE2010，12，46： | 1 |
| 10 | CCL18 from Tumor-Associated Macrophages Promotes Breast Cancer Metastasis via PITPNM3显示文摘 | Jingqi Chen Yandan Yao Chang Gong Fengyan Yu Shicheng Su Jianing Chen Bodu Liu Hui Deng Fengsong Wang Ling Lin Herui Yao Fengxi Su Karen S. Anderson Qiang Liu Mark E. Ewen Xuebiao Yao Erwei Song | 2011 | Cancer Cell2011，，4： | 1 |
| 11 | Use of surfacecoatings with natamycin to improve the storability of Hami melon atambient temperature显示文摘 | CONG Fengsong ZHANG Yungui DONG Wenyan | 2007 | Postharvest Biology and Technology2007，46，1： | 1 |
| 12 | Clinical Study on the Anti-Aging Effect of a Cream Containing Ginseng Root Extract and L-Carnosine显示文摘The anti-aging effects of a cream containing L-carnosine and ginseng root extract to provide a reference for the evaluation and development of anti-aging cosmetics were tested. Sixty healthy females, aged 35-60,were grouped into test sample and placebo groups. Skin elasticity, gloss, and wrinkles of the participants were analyzed before use and after use for 8 weeks. Instrument measurement results showed that after 8 weeks,skin elasticity, gloss, and wrinkles in the members of the test sample group obviously improved relative to those of the placebo group. Clinical effect assessment results showed that subjects did not experience adverse skin reactions, and their skin gloss showed obvious improvement. Self-assessment results showed that 87%subjects are satisfied with the test sample. Cream containing L-carnosine and ginseng root extract has a certain anti-aging effect. | Sun Hongmei Dai Jieling Yang Qiongli Li Yingyi Liu Fengsong | 2018 | China Detergent & Cosmetics2018，3，3： | 1 |
| 13 | Photoluminescence properties of alkaline metallic ions doped sol-gel silica glasses 显示文摘 | Ping yang Meng Kailu Chun Fengsong | 2002 | Materials Science and Engineering2002，90，12： | 1 |
| 14 | Acetylation of ezrin regulates membrane–cytoskeleton interaction underlying CCL18-elicited cell migration显示文摘Ezrin,a membrane–cytoskeleton linker protein,plays an essential role in cell polarity establishment,cell migration,and division.Recent studies show that ezrin phosphorylation regulates breast cancer metastasis by promoting cancer cell survivor and promotes intrahepatic metastasis via cell migration.However,it was less characterized whether there are additional post-translational modifications and/or post-translational crosstalks on ezrin underlying context-dependent breast cancer cell migration and invasion.Here we show that ezrin is acetylated by p300/CBP-associated factor(PCAF)in breast cancer cells in response to CCL18 stimulation.Ezrin physically interacts with PCAF and is a cognate substrate of PCAF.The acetylation site of ezrin was mapped by mass spectrometric analyses,and dynamic acetylation of ezrin is essential for CCL18-induced breast cancer cell migration and invasion.Mechanistically,the acetylation reduced the lipid-binding activity of ezrin to ensure a robust and dynamic cycling between the plasma membrane and cytosol in response to CCL18 stimulation.Biochemical analyses show that ezrin acetylation prevents the phosphorylation of Thr567.Using atomic force microscopic measurements,our study revealed that acetylation of ezrin induced its unfolding into a dominant structure,which prevents ezrin phosphorylation at Thr567.Thus,these results present a previously undefined mechanism by which CCL18-elicited crosstalks between the acetylation and phosphorylation on ezrin control breast cancer cell migration and invasion.This suggests that targeting PCAF signaling could be a potential therapeutic strategy for combating hyperactive ezrin-driven cancer progression. | Xiaoyu Song Wanjuan Wang Haowei Wang Xiao Yuan Fengrui Yang Lingli Zhao McKay Mullen Shihao Du Najdat Zohbi Saravanakumar Muthusamy Yalei Cao Jiying Jiang Peng Xia Ping He Mingrui Ding Nerimah Emmett Mingming Ma Quan Wu Hadiyah-Nicole Green Xia Ding Dongmei Wang Fengsong Wang Xing Liu | 2020 | Journal of Molecular Cell Biology2020，12，6： | 1 |
| 15 | IRE1-RACK1 axis orchestrates ER stress preconditioning-elicited cytoprotection from ischemia/reperfusion injury in liver显示文摘Endoplasmic reticulum(ER)stress is involved in ischemic preconditioning that protects various organs from ischemia/reperfusion(I/R)injury.We established an in vivo ER stress preconditioning model in which tunicamycin was injected into rats before hepatic I/R.The hepatic I/R injury,demonstrated by serum aminotransferase level and the ultra-structure of the liver,was alleviated by administration of tunicamycin,which induced ER stress in rat liver by activating inositol-requiring enzyme1(IRE1)andupregulating78 kDaglucose-regulated protein(GRP78).The proteomic identification for IRE1 binders revealed interaction and cooperation among receptor for activated C kinase 1(RACK1),phosphorylated AMPK,and IRE1 under ER stress conditions in a spatiotemporal manner.Furthermore,in vitro ER stress preconditioningwas induced by thapsigargin and tunicamycin in L02 and Hep G2 cells.Surprisingly,BCL2 was found to bephosphorylated by IRE1 under ER stress conditions to prevent apoptotic process by activation of autophagy.In conclusion,ER stress preconditioning protects against hepatic I/R injury,which is orchestrated by IRE1-RACK1 axis through the activation of BCL2.Our findings provide novel insights into the molecular pathways underlying ER stress preconditioning-elicited cytoprotective effect against hepatic I/R injury. | Dong Liu Xing Liu Ti Zhou William Yao Jun Zhao Zhigang Zheng Wei Jiang Fengsong Wang Felix O.Aikhionbare Donald L.Hill Nerimah Emmett Zhen Guo Dongmei Wang Xuebiao Yao Yong Chen | 2016 | Journal of Molecular Cell Biology2016，8，2： | 1 |
| 16 | Molecular cloning and expression analysis of crustin-like gene from chinese shrimp, Fenneropenaeus chinensis, 显示文摘 | LIU Fengsong LI Fuhua XIANG Jianhai | 2007 | Journal of Biotechnology2007，127，4： | 1 |
| 17 | Effects of long-term fast charging on a layered cathode for lithium-ion batteries显示文摘Fast charging, which aims to shorten recharge times to 10–15 min, is crucial for electric vehicles(EVs),but battery capacity usually decays rapidly if batteries are charged under such severe conditions.Revealing the failure mechanism is a prerequisite to improving the charging performance of lithium(Li)-ion batteries. Previous studies have focused less on cathode materials while also mostly focusing on their early changes. Thus, the cumulative effect of long-term fast charging on cathode materials has not been fully studied. Here, we study the changes in a layered cathode material during 1000 cycles of 6 C charging based on 1.6 Ah LiCoO_(2)/graphite pouch cells. Postmortem analysis reveals that the surface structure, charge transfer resistance and Li-ion diffusion coefficient of the cathode degenerate during repeated fast charging, causing a large increase in polarization. This polarization-induced poor utilization of the Li inventory is an important reason for the rapid capacity fading of batteries. These findings deepen the understanding of the aging mechanism for cells undergoing fast charging and can be used as benchmarks for the future development of high-capacity, fast-charging layered cathode materials. | Jingwei Hu Fengsong Fan Qian Zhang Shengwen Zhong Quanxin Ma | 2022 | Journal of Energy Chemistry2022，31，4： | 1 |
| 18 | Molecular cloning and expression profile of putative anti-lipopolysaceharide factor (ALF) in Chinese shrimp(Fenneropenaeuschinensis) 显示文摘 | LIU Fengsong LIU Yichen LI Fuhua | 2005 | Marine Biotechnology2005，7，6： | 1 |
| 19 | Molecular cloning and expression profile of putative anti-lipopolysaccharide factor (ALF) in Chinese shrimp (Fenneropenaeus chinensis) 显示文摘 | LIU Fengsong LIU Yichen LI Fuhua | 2005 | Marine Biotechnology2005，7，6： | 1 |
| 20 | Photoluminescence properties of alkaline metallic ions doped sol-gel silica glasses 显示文摘 | Ping yang Meng Kailu Chun Fengsong | 2002 | Materials Seience and Engineering2002，90，12： | 1 |
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