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| 1 | Integration of lipidomics and metabolomics for in-depth understanding of cellular mechanism and disease progression显示文摘Mass spectrometry(MS)-based omics technologies are now widely used to profile small molecules in multiple matrices to confer comprehensive snapshots of cellular metabolic phenotypes.The metabolomes of cells,tissues,and organisms comprise a variety of molecules including lipids,amino acids,sugars,organic acids,and so on.Metabolomics mainly focus on the hydrophilic classes,while lipidomics has emerged as an independent omics owing to the complexities of the organismal lipidomes.The potential roles of lipids and small metabolites in disease pathogenesis have been widely investigated in various human diseases,but system-level understanding is largely lacking,which could be partly attributed to the insufficiency in terms of metabolite coverage and quantitation accuracy in current analytical technologies.While scientists are continuously striving to develop high-coverage omics approaches,integration of metabolomics and lipidomics is becoming an emerging approach to mechanistic investigation.Integration of metabolome and lipidome offers a complete atlas of the metabolic landscape,enabling comprehensive network analysis to identify critical metabolic drivers in disease pathology,facilitating the study of interconnection between lipids and other metabolites in disease progression.In this review,we summarize omics-based findings on the roles of lipids and metabolites in the pathogenesis of selected major diseases threatening public health.We also discuss the advantages of integrating lipidomics and metabolomics for in-depth understanding of molecular mechanism in disease pathogenesis. | Raoxu Wang Bowen Li Sin Man Lam Guanghou Shui | 2020 | Journal of Genetics and Genomics2020，47，2： | 15 |
| 2 | Lipidomics as a Principal Tool for Advancing Biomedical Research显示文摘Lipidomics,在 lipidome 的一张全面地图的构造指向在一个房间或纸巾以内包括全部类脂化合物水池,当前在类脂化合物生物学,技术和药的接口作为一个独立学科正在出现。生物 lipidomes 的差异和复杂性打电话让技术革新和改进满足各种各样的生物医学的研究的需要。特别地, lipidomic 研究的领域里的扩大的最近的波浪主要在分析技术被归因于进展为在细胞的 lipidome 的多样的类脂化合物种类的宽数组的描述和 quantification 的新集体度谱、色析法的工具的开发。这里,我们在 lipidome 分析考察一些关键技术进展并且向前把 lipidomics 的应用放在包括新陈代谢的症候群, neurodegenerative 疾病和传染疾病在众多的疾病模型探讨类脂化合物的生物角色,为为生物医学的研究有用的各种各样的 mammalian/organism 模型象增加的紧急一样盘点构造 lipidome 。 | Sin Man Lam Guanghou Shui | 2013 | Journal of Genetics and Genomics2013，40，8： | 10 |
| 3 | Mea6 controls VLDL transport through the coordinated regulation of COPil assembly显示文摘 | Yaqing Wang Liang Liu Hongsheng Zhang Junwan Fan FengZhang Mei Yu Lei Shi Lin Yang Sin Man Lam Huimin Wang Xiaowei Chen Yingchun Wang Fei Gao Guanghou Shui Zhiheng Xu | 2016 | Cell Research2016，26，7： | 4 |
| 4 | Metabolomics through the lens of precision cardiovascular medicine显示文摘Metabolomics, which targets at the extensive characterization and quantitation of global metabolites from both endogenous and exogenous sources, has emerged as a novel technological avenue to advance the field of precision medicine principally driven by genomics-oriented approaches. In particular,metabolomics has revealed the cardinal roles that the environment exerts in driving the progression of major diseases threatening public health. Herein, the existent and potential applications of metabolomics in two key areas of precision cardiovascular medicine will be critically discussed: 1) the use of metabolomics in unveiling novel disease biomarkers and pathological pathways; 2) the contribution of metabolomics in cardiovascular drug development. Major issues concerning the statistical handling of big data generated by metabolomics, as well as its interpretation, will be briefly addressed. Finally, the need for integration of various omics branches and adopting a multi-omics approach to precision medicine will be discussed. | Sin Man Lam Yuan Wang Bowen Li Jie Du Guanghou Shui | 2017 | Journal of Genetics and Genomics2017，44，3： | 3 |
| 5 | ACBD3 is required for FAPP2 transferring glucosylceramide through maintaining the Golgi integrity显示文摘Glycosphingolipid (GSL) metabolism is involved in various physiological processes, including all major cell signaling pathways, and its dysregulation is linked to some diseases. The four-phosphate adaptor protein FAPP2-mediated glucosylceramide (GlcCer) transport for complex GSL synthesis has been studied extensively. However, the molecular machinery of FAPP2 as a GlcCer-transferring protein remains poorly defined. Here, we identify a Golgi-resident protein, acyl-coenzyme A binding domain containing 3 (ACBD3), as an interacting partner of FAPP2. We find that ACBD3 knockdown leads to dramatic Golgi fragmentation, which subsequently causes FAPP2 dispersal throughout the cytoplasm and a decreased localization at trans-Golgi network. The further quantitative Upidomic analysis indicates that ACBD3 knockdown triggers abnormal sphingolipid metabolism. Interestingly, the expression of siRNA-resistant full-length ACBD3 can rescue these defects caused by ACBD3 knockdown. These data reveal critical roles for ACBD3 in maintaining the integrity of Golgi morphology and cellular sphingolipid homeostasis and establish the importance of the integrated Golgi complex for the transfer of GlcCer and complex GSL synthesis. | Jing Liao Yuxiang Guan Wei Chen Can Shi Dongdong Yao Fengsong Wang Sin Man Lam Guanghou Shui Xinwang Cao | 2019 | Journal of Molecular Cell Biology2019，11，2： | 3 |
| 6 | GM130 regulates pulmonary surfactant protein secretion in alveolar typeⅡcells显示文摘Pulmonary surfactant is a lipid-protein complex secreted by alveolar typeⅡepithelial cells and is essential for the maintenance of the delicate structure of mammalian alveoli to promote efficient gas exchange across the air-liquid barrier.The Golgi apparatus plays an important role in pulmonary surfactant modification and secretory trafficking.However,the physiological function of the Golgi apparatus in the transport of pulmonary surfactants is unclear.In the present study,deletion of GM130,which encodes for a matrix protein of the cis-Golgi cisternae,was shown to induce the disruption of the Golgi structure leading to impaired secretion of lung surfactant proteins and lipids.Specifically,the results of in vitro and in vivo analysis indicated that the loss of GM130 resulted in trapping of Sftpa in the endoplasmic reticulum,Sftpb and Sftpc accumulation in the Golgi apparatus,and an increase in the compensatory secretion of Sftpd.Moreover,global and epithelial-specific GM130 knockout in mice resulted in an enlargement of alveolar airspace and an increase in alveolar epithelial autophagy;however,surfactant repletion partially rescued the enlarged airspace defects in GM130-deficient mice.Therefore,our results demonstrate that GM130 and the mammalian Golgi apparatus play a critical role in the control of surfactant protein secretion in pulmonary epithelial cells. | Qianqian Pang Chunyi Liu Yulong Qiao Jian Zhao Sin Man Lam Mei Mei Guanghou Shui Shilai Bao Qiuling Li | 2022 | Science China(Life Sciences)2022，65，1： | 2 |
| 7 | Comprehensive lipidomics in apoM^(-/-) mice reveals an overall state of metabolic distress and attenuated hepatic lipid secretion into the circulation显示文摘Apolipoprotein M(apoM) participates in both high-density lipoprotein and cholesterol metabolism. Little is known about how apo M affects lipid composition of the liver and serum. In this study, we systemically investigated the effects of apo M on liver and plasma lipidomes and how apo M participates in lipid cycling, via apo M knockout in mice and the human SMMC-7721 cell line. We used integrated mass spectrometry-based lipidomics approaches to semiquantify more than 600 lipid species from various lipid classes, which include free fatty acids, glycerolipids, phospholipids, sphingolipids, glycosphingolipids, cholesterol, and cholesteryl esters(CEs), in apo M^(-/-)mouse. Hepatic accumulation of neutral lipids, including CEs, triacylglycerols, and diacylglycerols, was observed in apo M^(-/-)mice;while serum lipidomic analyses showed that, in contrast to the liver, the overall levels of CEs and saturated/monounsaturated fatty acids were markedly diminished. Furthermore, the level of Apo B-100 was dramatically increased in the liver, whereas significant reductions in both Apo B-100 and low-density lipoprotein(LDL) cholesterol were observed in the serum of apo M^(-/-)mice, which indicated attenuated hepatic LDL secretion into the circulation. Lipid profiles and proinflammatory cytokine levels indicated that apo M^(-/-)leads to hepatic steatosis and an overall state of metabolic distress. Taken together, these results revealed that apo M knockout leads to hepatic steatosis, impaired lipid secretion, and an overall state of metabolic distress. | Yuanping Shi Sin Man Lam Hong Liu Guanghua Luo Jun Zhang Shuang Yao Jie Li Lu Zheng Ning Xu Xiaoying Zhang Guanghou Shui | 2020 | Journal of Genetics and Genomics2020，47，9： | 2 |
| 8 | Separation and determination of organic acids and phenolic compounds in fruit juices and drinks by high-performance liquid chromatography显示文摘 | Guanghou Shui Laipeng Leong | 2002 | Journal of Chromatography A2002，977，： | 1 |
| 9 | Rnf20 deficiency in adipocyte impairs adipose tissue development and thermogenesis显示文摘RNF20,an E3 ligase critical for monoubiquitination of histone H2B at lysine 120(H2Bub),has been implicated in the regulation of various cellar processes;however,its physiological roles in adipocytes remain poorly characterized.Here,we report that the adipocyte-specific knockout of Rnf20(ASKO)in mice led to progressive fat toss,organomegaly and hyperinsulinemia.Despite signs of hyperinsulinemia,normal insulin sensitivity and improved glucose tolerance were observed in the young and aged CD-fed ASKO mice.In addition,high-fat diet-fed ASKO mice developed severe liver steatosis.Moreover,we observed that the ASKO mice were extremely sensitive to a cold environment due to decreased expression levels of brown adipose tissue(BAT)selective genes,including uncoupling protein 1(l/cpf),and impaired mitochondrial functions.Significantly decreased levels of peroxisome proliterator-activated receptor gamma(Ppary)were observed in the gonadal white adipose tissues(gWAT)from the ASKO mice,suggesting that Rnf20 regulates adipogenesis,at least in part,through Ppary.Rosiglitazone-treated ASKO mice exhibited increased fat mass compared to that of the non-treated ASKO mice.Collectively,our results illustrate the critical role of RNF20 in control of white and brown adipose tissue development and physiological function. | Xiaojuan Liang Cong Tao Jianfei Pan Lilan Zhang Lulu Liu Ying Zhao Yiping Fan Chunwei Cao Jiali Liu Jin Zhang Sin Man Lam Guanghou Shui Wanzhu Jin Wei Li Jianguo Zhao Kui Li Yanfang Wang | 2021 | Protein & Cell2021，12，6： | 1 |
| 10 | Separation and determination of organic acids and phenolic compounds in fruit juices and drinks by high-performance liquid chromatography显示文摘 | SHUI Guanghou LAI Pengleong | 2002 | Journal of Chromatography A2002，977，1： | 1 |
| 11 | Separation and determination of organic acids and phenolic compounds in fruit juices and drinks by high-performance liquid chromatography显示文摘 | GUANGHOU SHUI LAIPENG LEONG | 2002 | J Chromatography A2002，997，： | 1 |
| 12 | Separation and determination of organic acids and phenolic compounds in fruit juices and drinks by high performance liquid chromatography显示文摘 | Guanghou Shui Lai Peng Leong | 2002 | Journal of Chromatography A2002，977，： | 1 |
| 13 | Mesenteric lymph system constitutes the second route in gut-liver axis and transports metabolism-modulating gut microbial metabolites显示文摘The gut-liver axis denotes the intricate connection and interaction between gut microbiome and liver, in which compositional and functional shifts in gut microbiome affect host metabolism. Hepatic portal vein of the blood circulation system has been thought to be the major route for metabolite transportation in the gut-liver axis, but the existence and importance of other routes remain elusive. Here, we perform metabolome comparison in blood circulation and mesenteric lymph systems and identify significantly shifted metabolites in serum and mesentery. Using cellular assays, we find that the majority of decreased metabolites in lymph system under high-fat diet are effective in alleviating metabolic disorders, indicating a high potential of lymph system in regulating liver metabolism. Among those, a representative metabolite, L-carnitine, reduces diet-induced obesity in mice. Metabolic tracing analysis identifies that L-carnitine is independently transported by the mesenteric lymph system, serving as an example that lymph circulation comprises a second route in the gut-liver axis to modulate liver metabolism. Our study provides new insights into metabolite transportation via mesenteric lymph system in the gut-liver axis, offers an extended scope for the investigations in host-gut microbiota metabolic interactions and potentially new targets in the treatment of metabolic disorders. | Ying Yu Bin Liu Xiaolin Liu Xuan Zhang Wenhui Zhang He Tian Guanghou Shui Wenzhao Wang Moshi Song Jun Wang | 2022 | Journal of Genetics and Genomics2022，49，7： | 1 |
| 14 | Separation and determination of organic acids and phenolic compounds in fruit juices and drinks by HPLC显示文摘 | Shui Guanghou Leong L P | 2002 | Journal of Chromatography A2002，，： | 1 |
| 15 | Separation and determination of organic acids and phenolic compounds in fi-uit juices and drinks by HPLC显示文摘 | Shui Guanghou Leong L P | 2002 | Journal of Chromatography A2002，977，： | 1 |
| 16 | An improved method for the analysis of major antioxidants of Hibiscus esculentus Linn 显示文摘 | Shui Guanghou Peng Leonglai | 2004 | Journal of Chromatography A12004，1048，1： | 1 |
| 17 | Emodin Inhibits tumor cell adhesion through disruption of the membrane lipid raft-associated integrin signaling pathway 显示文摘 | Qing Huang Han-Ming Shen Guanghou Shui etal | 2006 | Cancer Research2006，66，： | 1 |
| 18 | Characterization of antioxidants and change of antioxidant levels during storage of Manilkara zapotal显示文摘 | Shui Guanghou Wong Shih Peng Leong Lai Peng | 2004 | Journal of Agricultural and Food Chemistry2004，，1： | 1 |
| 19 | Separation and determination of organic aeids and phenolic compounds in fruit juices and drinks by high-performance liquid chromatography显示文摘 | Shui Guanghou Long Laipeng | 2002 | Journal of Chromatography A2002，977，： | 1 |
| 20 | A harlequin ichthyosis pig model with a novel ABCA12 mutation can be rescued by acitretin treatment显示文摘Harlequin ichthyosis (HI) is a severe genetic skin disorder and caused by mutation in the ATP-binding cassette A12 (ABCA12) gene. The retinoid administration has dramatically improved long-term survival of HI, but improvements are still needed. However, the ABCA12 null mice failed to respond to retinoid treatment, which impedes the development of novel cure strategies for HI. Here we generated an ethylnitrosourea mutagenic HI pig model (named Z9), which carries a novel deep intronic mutation IVS49-727 A>G in the ABCA12 gene, resulting in abnormal mRNA splicing and truncated protein production. Z9 pigs exhibit significant clinical symptom as human patients with HI. Most importantly, systemic retinoid treatment significantly prolonged the life span of the mutant pigs via improving epidermal maturation, decreasing epidermal apoptosis, and triggering the expression of ABCA6. Taken together, this pig model perfectly resembles the clinical symptom and molecular pathology of patients with HI and will be useful for understanding mechanistic insight and developing therapeutic strategies. | Xiao Wang Chunwei Cao Yongshun Li Tang Hai Qitao Jia Ying Zhang Qiantao Zheng Jing Yao Guosong Qin Hongyong Zhang Ruigao Song Yanfang Wang Guanghou Shui Sin Man Lam Zhonghua Liu Hong Wei Anming Meng Qi Zhou Jianguo Zhao | 2019 | Journal of Molecular Cell Biology2019，11，12： | 1 |
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