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| 1 | PRMT5 regulates Golgi apparatus structure through methylation of the golgin GM130显示文摘Golgi 仪器(GA ) 的维护结构和功能取决于 Golgi 矩阵蛋白质。象 golgin 和掌握家庭的成员的 phosphorylation 那样的 Golgi 蛋白质的 posttranslational 修正为决定 Golgi 建筑学是重要的。包括 golgin-84 的一些 Golgi 蛋白质也被知道是 methylated,但是 golgin methylation 的功能仍然保持不清楚。这里,我们证明蛋白质精氨酸 methyltransferase (PRMT5 ) 5 本地化到 GA 和形式建筑群,几个部件在拴住的 GA 带子形成和泡包含了。PRMT5 在 Golgi 带子形成与 PRMT5 原因缺点的 golgin GM130,和弄空交往。而且,在 GM130 的 PRMT5 甲醇化物 N 终端精氨酸,和如此的精氨酸 methylation 为 GA 显得批评带子形成。我们的调查结果揭示 GM130 的 PRMT5 依赖的精氨酸 methylation 由控制 GA 建筑学的维护的分子的机制。 | Zhongwei Zhou Xiaotian Sun Zhenhua Zou Litao Sun Tao Zhang Shaoshi Guo Ya Wen Lin Liu Yi Wang Jun Qin Lei Li Weimin Gong Shilai Bao | 2010 | Cell Research2010，20，9： | 16 |
| 2 | The tea plant CsLHT1 and CsLHT6 transporters take up amino acids, as a nitrogen source, from the soil of organic tea plantations显示文摘Organic tea is more popular than conventional tea that originates from fertilized plants.Amino acids inorganic soils constitute a substantial pool nitrogen(N)available for plants.However,the amino-acid contents in soils of tea plantations and how tea plants take up these amino acids remain largely unknown.In this study,we show that the amino-acid content in the soil of an organic tea plantation is significantly higher than that of a conventional tea plantation.Glutamate,alanine,valine,and leucine were the most abundant amino acids in the soil of this tea plantation.When 15 N-glutamate was fed to tea plants,it was efficiently absorbed and significantly increased the contents of other amino acids in the roots.We cloned seven CsLHT genes encoding amino-acid transporters and found that the expression of CsLHT1,CsLHT2,and CsLHT6 in the roots significantly increased upon glutamate feeding.Moreover,the expression of CsLHT1 or CsLHT6 in a yeast amino-acid uptake-defective mutant,22Δ10α,enabled growth on media with amino acids constituting the sole N source.Amino-acid uptake assays indicated that CsLHT1 and CsLHT6 are H^(+)-dependent high-and low-affinity amino-acid transporters,respectively.We further demonstrated that CsLHT1 and CsLHT6 are highly expressed in the roots and are localized to the plasma membrane.Moreover,overexpression of CsLHT1 and CsLHT6 in Arabidopsis significantly improved the uptake of exogenously supplied 15 N-glutamate and 15 N-glutamine.Taken together,our findings are consistent with the involvement of CsLHT1 and CsLHT6 in amino-acid uptake from the soil,which is particularly important for tea plants grown inorganic tea plantations. | Fang Li Chunxia Dong Tianyuan Yang Shilai Bao Wanping Fang William J.Lucas Zhaoliang Zhang | 2021 | Horticulture Research2021，8，1： | 3 |
| 3 | GM130 regulates pulmonary surfactant protein secretion in alveolar typeⅡcells显示文摘Pulmonary surfactant is a lipid-protein complex secreted by alveolar typeⅡepithelial cells and is essential for the maintenance of the delicate structure of mammalian alveoli to promote efficient gas exchange across the air-liquid barrier.The Golgi apparatus plays an important role in pulmonary surfactant modification and secretory trafficking.However,the physiological function of the Golgi apparatus in the transport of pulmonary surfactants is unclear.In the present study,deletion of GM130,which encodes for a matrix protein of the cis-Golgi cisternae,was shown to induce the disruption of the Golgi structure leading to impaired secretion of lung surfactant proteins and lipids.Specifically,the results of in vitro and in vivo analysis indicated that the loss of GM130 resulted in trapping of Sftpa in the endoplasmic reticulum,Sftpb and Sftpc accumulation in the Golgi apparatus,and an increase in the compensatory secretion of Sftpd.Moreover,global and epithelial-specific GM130 knockout in mice resulted in an enlargement of alveolar airspace and an increase in alveolar epithelial autophagy;however,surfactant repletion partially rescued the enlarged airspace defects in GM130-deficient mice.Therefore,our results demonstrate that GM130 and the mammalian Golgi apparatus play a critical role in the control of surfactant protein secretion in pulmonary epithelial cells. | Qianqian Pang Chunyi Liu Yulong Qiao Jian Zhao Sin Man Lam Mei Mei Guanghou Shui Shilai Bao Qiuling Li | 2022 | Science China(Life Sciences)2022，65，1： | 2 |
| 4 | IκB Kinase Promotes Tumorigenesis through Inhibition of Forkhead FOXO3a显示文摘 | Mickey C.-T Hu Dung-Fang Lee Weiya Xia Leonard S Golfman Fu Ou-Yang Jer-Yen Yang Yiyu Zou Shilai Bao Norihisa Hanada Hitomi Saso Ryuji Kobayashi Mien-Chie Hung | 2004 | Cell2004，，2： | 2 |
| 5 | The Highly Conserved Protein Methyltransferase, Skbl, Is a Mediator of Hyperosmotic Stress Response in the Fission Yeast Schizossaccharomycespombe 显示文摘 | Shilai Bao Peirong Yang HyeWon Kim | 2001 | J Biol Chem2001，276，14： | 1 |
| 6 | IκB Kinase Promotes Tumorigenesis through Inhibition of Forkhead FOXO3a显示文摘 | Mickey C.-T Hu Dung-Fang Lee Weiya Xia Leonard S Golfman Fu Ou-Yang Jer-Yen Yang Yiyu Zou Shilai Bao Norihisa Hanada Hitomi Saso Ryuji Kobayashi Mien-Chie Hung | 2004 | Cell2004，，2： | 1 |
| 7 | PRMT5 determines the pattern of polyploidization and prevents liver from cirrhosis and carcinogenesis显示文摘Human hepatocellular carcinoma(HCC)occurs almost exclusively in cirrhotic livers.Here,we report that hepatic loss of protein arginine methyltransferase 5(PRMT5)in mice is sufficient to cause cirrhosis and HCC in a clinically relevant way.Furthermore,pathological polyploidization induced by hepatic loss of PRMT5 promotes liver cirrhosis and hepatic tumorigenesis in aged liver.The loss of PRMT5 leads to hyperaccumulation of P21 and endoreplication-dependent formation of pathological mono-nuclear polyploid hepatocytes.PRMT5 and symmetric dimethylation at histone H4 arginine 3(H4R3me2s)directly associate with chromatin of P21 to suppress its transcription.More importantly,loss of P21 rescues the pathological mono-nuclear polyploidy and prevents PRMT5-deficiency-induced liver cirrhosis and HCC.Thus,our results indicate that PRMT5-mediated symmetric dimethylation at histone H4 arginine 3(H4R3me2s)is crucial for preventing pathological polyploidization,liver cirrhosis and tumorigenesis in mouse liver. | Jincheng Wang Xiang Huang Daoshan Zheng Qiuling Li Mei Mei Shilai Bao | 2023 | Journal of Genetics and Genomics2023，50，2： | 1 |
| 8 | The Protein Arginine Methylase 5(PRMT5/SKB1) Gene Is Required for the Maintenance of Root Stem Cells in Response to DNA Damage显示文摘Plant root stem cells and their surrounding microenvironment,namely the stem cell niche,are hypersensitive to DNA damage.However,the molecular mechanisms that help maintain the genome stability of root stem cells remain elusive.Here we show that the root stem cells in the skbl(Shk1 kinase binding protein 1) mutant undergoes DNA damage-induced cell death,which is enhanced when combined with a lesion of the Ataxia-telangiectasia mutated(ATM) or the ATM/RAD3-related(ATR) genes,suggesting that the SKBI plays a synergistically effect with ATM and ATR in DNA damage pathway.We also provide evidence that SKBI is required for the maintenance of quiescent center(QC),a root stem cell niche,under DNA damage treatments.Furthermore,we report decreased and ectopic expression of SHORTROOT(SHR) in response to DNA damage in the skbl root tips,while the expression of SCARECROW(SCR) remains unaffected.Our results uncover a new mechanism of plant root stem cell maintenance under DNA damage conditions that requires SKB1. | Qiuling Li Yan Zhao Minghui Yue Yongbiao Xue Shilai Bao | 2016 | Journal of Genetics and Genomics2016，43，4： | 1 |
| 9 | Haem Oxygenase 1 is a potential target for creating etiolated/albino tea plants (Camellia sinensis) with high theanine accumulation显示文摘Theanine content is highly correlated with sensory quality and health benefits of tea infusion.The tender shoots of etiolated and albino tea plants contain higher theanine than the normal green tea plants and are valuable materials for high quality green tea processing.However,why these etiolated or albino tea plants can highly accumulate theanine is largely unknown.In this study,we observed an Arabidopsis etiolated mutant hy1–100(mutation in Haem Oxygenase 1,HO1)that accumulated higher levels of glutamine(an analog of theanine).We therefore identified CsHO1 in tea plants and found CsHO1 is conserved in amino acid sequences and subcellular localization with its homologs in other plants.Importantly,CsHO1 expression in the new shoots was much lower in an etiolated tea plants‘Huangkui’and an albino tea plant‘Huangshan Baicha’than that in normal green tea plants.The expression levels of CsHO1 were negatively correlated with theanine contents in these green,etiolated and albino shoots.Moreover,CsHO1 expression levels in various organs and different time points were also negatively correlated with theanine accumulation.The hy1–100 was hypersensitive to high levels of theanine and accumulated more theanine under theanine feeding,and these phenotypes were rescued by the expression of CsHO1 in this mutant.Transient knockdown CsHO1 expression in the new shoots of tea plant using antisense oligonucleotides(asODN)increased theanine accumulation.Collectively,these results demonstrated CsHO1 negatively regulates theanine accumulation in tea plants,and that low expression CsHO1 likely contributes to the theanine accumulation in etiolated/albino tea plants. | Ziping Chen Shijia Lin Tingting Chen Mengxue Han Tianyuan Yang Yan Wang Shilai Bao Zhougao Shen Xiaochun Wan Zhaoliang Zhang | 2023 | Horticulture Research2023，10，2： | 0 |
| 10 | The ABA synthesis enzyme allele OsNCED2^(T)promotes dryland adaptation in upland rice显示文摘Upland rice shows dryland adaptation in the form of a deeper and denser root system and greater drought resistance than its counterpart,irrigated rice.Our previous study revealed a difference in the frequency of the OsNCED2 gene between upland and irrigated populations.A nonsynonymous mutation(C to T,from irrigated to upland rice)may have led to functional variation fixed by artificial selection,but the exact biological function in dryland adaptation is unclear.In this study,transgenic and association analysis indicated that the domesticated fixed mutation caused functional variation in OsNCED2,increasing ABA levels,root development,and drought tolerance in upland rice under dryland conditions.OsNCED2-overexpressing rice showed increased reactive oxygen species-scavenging abilities and transcription levels of many genes functioning in stress response and development that may regulate root development and drought tolerance.OsNCED2^(T)-NILs showed a denser root system and drought resistance,promoting the yield of rice under dryland conditions.OsNCED2^(T)may confer dryland adaptation in upland rice and may find use in breeding dryland-adapted,water-saving rice. | Liyu Huang Yachong Bao Shiwen Qin Min Ning Qinyan Li Qingmao Li Shilai Zhang Guangfu Huang Jing Zhang Wensheng Wang Binying Fu Fengyi Hu | 2024 | The Crop Journal2024，12，1： | 0 |
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